CN101779681B - Insect repellant containing extract of witloof ethyl acetate and preparation thereof - Google Patents

Abstract

The invention discloses an insect repellent containing chicory ethyl acetate extract and a preparation method thereof. The prepared insect repellent is prepared by mixing the following raw materials in parts by weight: chicory ethyl acetate extract: 0.30-0.34 parts , corn leaf powder: 2.0-2.2 parts, wheat bran powder: 0.9-1.1 parts, yeast: 0.8-0.9 parts, agar: 0.40-0.45 parts, multivitamins: 0.30-0.34 parts, water 22-27 parts. It not only protects the ecology, but also has no environmental pollution. The insect repellent has about two-thirds of the lethal effect on armyworms; while the remaining one-third is effectively inhibited from growing, prolonging the growth period by 6-7 days, so that armyworms are staggered from the farming season and reduce the number of pests. Harm to crops, while protecting the ecology without destroying the biological chain. The production cost of the insect repellent is low, green and environment-friendly, and has great market prospect in agricultural production practice.

Description

An insect repellant containing chicory ethyl acetate extract and its preparation

technical field

The invention mainly relates to an insect repellent and its preparation, in particular to an insect repellent containing chicory ethyl acetate extract and its preparation method.

Background technique

Chicory (Cichorium intybus L.) is a perennial herb of the genus Cichorium in the Asteraceae family. It is native to Europe, West Asia, Central Asia and North America, and is distributed in Northwest, North and Northeast my country. Chicory has high nutritional value, resistance to diseases and insect pests, high yield, and strong regeneration ability. It is a plant resource with great development potential. The natural products extracted from chicory are widely used as feed green additives, natural food ingredients, drug carriers and vaccine adjuvants.

Existing various insecticidal and anthelmintic pesticides used on vegetables, forest fruits, tea leaves and crops all have certain degree of toxicity and side effects. Its toxic components will cause land, water and biological pollution to varying degrees. Today, when green agriculture is advocated, it is imperative and imperative to replace traditional pesticides with pure plant natural product insect repellents to reduce environmental pollution.

Compositae is one of the most important plant categories in the development of botanical green pesticides. The natural products of chicory extract contain many types of chemical components, mainly sesquiterpene lactones, diterpenes, sesquiterpenes, monoterpenes, flavonoids, coumarins and sterols, etc. The variable structural type and spatial configuration make the plant exhibit a wide range of biological activities. Pharmacological and pharmacodynamic studies have shown that the organic solvent extract of the plant has good cytotoxicity, insecticidal and anthelmintic effects. Therefore, the development of green insect repellent with chicory natural product extract not only protects the ecology, but also has no environmental pollution.

Contents of the invention

The object of the present invention is to provide a kind of green insect repellant containing chicory natural product extract and its preparation method. The prepared insect repellent can not only protect ecology, but also has no environmental pollution.

In order to realize above-mentioned task, the present invention takes following technical solution:

An insect repellent containing chicory ethyl acetate extract is characterized in that the prepared insect repellent is mixed with the following raw materials in parts by weight:

Chicory ethyl acetate extract: 0.30-0.34 parts, corn leaf powder: 2.0-2.2 parts, wheat bran powder: 0.9-1.1 parts, yeast: 0.8-0.9 parts, agar: 0.40-0.45 parts, multivitamins: 0.30-0.34 parts parts, 22 to 27 parts of water.

The preparation method of the above-mentioned insect repellent containing chicory ethyl acetate extract is characterized in that, comprising the following steps:

1) Dig the whole plant of chicory, separate the roots from the stems and leaves, and clean them; cut the roots and stems into small pieces about 1cm long with a knife, dry them in the shade indoors, and then place them in an electric heating constant temperature blast drying oven at 35°C~ 40°C, 24-48 hours to constant weight; pulverize with a plant sample pulverizer, and pass through a 40-mesh sieve to obtain chicory plant dry powder;

2) Weigh 1 part of the above-mentioned chicory plant dry powder, add 10-11 parts by weight of ethyl acetate solvent, soak for 48 hours, use ultrasonic vibration for 0.5 hours, after standing for 24 hours, use ultrasonic vibration for 0.5 hours again, and then let stand , filtered; the filtrate was evaporated, concentrated and extracted at a temperature of 75.5°C to 77.5°C, so that the solid matter was continuously extracted by the solvent until a viscous paste was obtained, which was the chicory ethyl acetate extract;

3) Add the specified amount of chicory ethyl acetate extract, corn leaf powder, wheat bran powder, yeast, agar, and multivitamins into the water and mix.

The greatest advantage of the invention is that the insect repellent is prepared by using the chicory ethyl acetate extract, which not only protects the ecology, but also has no environmental pollution. It has been proved by experiments that the insect repellant has a lethal effect on armyworms by about two-thirds; while the remaining one-third is effectively inhibited from growing, prolonging the growth period by 6-7 days (see examples), It makes the armyworm stagger the farming time to reduce the damage to the crops, and at the same time protect the ecology without destroying the biological chain. The production cost of the insect repellent is low, green and environment-friendly, and has great market prospect in agricultural production practice.

Description of drawings

Fig. 1 is the flow chart of preparation of green insect repellant of the present invention.

The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

Detailed ways

According to the technical scheme of the present invention, the green insect repellent containing chicory ethyl acetate extract is mainly formed by mixing the following raw materials in parts by weight:

Chicory ethyl acetate extract: 0.30-0.34 parts;

Corn leaf powder: 2.0 to 2.2 parts;

Wheat bran powder: 0.9 to 1.1 parts;

Yeast: 0.8 to 0.9 parts;

Agar: 0.40～0.45 parts;

Multivitamins: 0.30-0.34 parts;

Water: 22-27 parts;

Referring to Fig. 1, the above-mentioned chicory ethyl acetate extract adopts the following steps to obtain:

① Preparation of chicory dry powder

Dig the whole plant of chicory, separate the roots from the stems and leaves, and clean them; cut the roots and stems into small pieces about 1cm long with a knife, dry them in the shade indoors, and then place them in an electric heating constant temperature blast drying oven at 35°C to 40°C , 24 to 48 hours to constant weight; pulverize with a plant sample pulverizer, and pass through a 40-mesh sieve to obtain chicory plant dry powder.

② Preparation of chicory ethyl acetate extract

Weigh 1 part of the above-mentioned chicory plant dry powder, add 10-11 parts by weight of ethyl acetate solvent, soak it for 48 hours, shake it with ultrasonic waves (power 200W, temperature 55-60°C) for 0.5 hours, let it stand for 24 hours, and use it again Ultrasound (power 200W, temperature 55-60°C) oscillates for 0.5 hours, then stands still, and filters; the filtrate is evaporated, concentrated and extracted at a temperature of 75.5°C-77.5°C, so that the solid matter is continuously extracted by the solvent until a viscous paste is obtained The product is chicory ethyl acetate extract.

Add water to chicory extract at a ratio of 1:75~100, and then add other ingredients in the formula to obtain a green insect repellent containing chicory ethyl acetate extract.

The following are the specific examples given by the inventor:

Product Preparation Example 1:

Take chicory ethyl acetate extract: 0.32 parts, corn leaf powder: 2.0 parts, wheat bran powder: 1 part, yeast: 0.8% parts, agar: 0.4 parts, multivitamins: 0.32 parts, water 25 parts;

Add water to the chicory ethyl acetate extract, then add other raw materials, and mix according to the conventional method.

Yeast, agar, and multivitamins are commercially available conventional products.

Product preparation embodiment 2:

Take chicory ethyl acetate extract: 0.30 parts, corn leaf powder: 2.2 parts, wheat bran powder: 1.1 parts, yeast: 0.9% parts, agar: 0.45 parts, multivitamins: 0.34 parts, water 27 parts;

Add water to the chicory ethyl acetate extract, then add other raw materials, and mix according to the conventional method.

Yeast, agar, and multivitamins are commercially available conventional products.

Product preparation embodiment 3:

Take chicory ethyl acetate extract: 0.34 parts, corn leaf powder: 2.1 parts, wheat bran powder: 0.9 parts, yeast: 0.8% parts, agar: 0.43 parts, multivitamins: 0.33 parts, water 22 parts;

Add water to the chicory ethyl acetate extract, then add other raw materials, and mix according to the conventional method.

Yeast, agar, and multivitamins are commercially available conventional products.

The implementation effect of the green insect repellant prepared by the above-described embodiment to armyworm (Mythimna separata Walker):

Take the above-mentioned green insect repellant and armyworm prepared respectively, armyworm is fed with artificial feed, and the armyworm with well-developed and known body weight is fed for treatment. Feed Handbook, 1984, published by Shanghai Science and Technology Press.

In the treatment group, an appropriate amount of green insect repellent was added to the petri dish, and in the control group, artificial feed was added. One 4-day-old test insect was fed to each dish and raised in an artificial climate box. The artificial climate box was set at 8 hours of light per day and a temperature of 25°C, and 16 hours of darkness at a temperature of 15°C; 30 heads per treatment were repeated 3 times, and the treatment groups were fed insect repellants from the initial day 0 to day 3, respectively. After the 5th day, the artificial feed was replaced; the control group was always fed with artificial feed. Continue until the test insects pupate, observe the feeding, growth and development, life and death reactions of the test insects in detail, and regularly weigh the test insect weights, count the pupation rate and mortality, and comprehensively evaluate the inhibition of the insect repellent on the growth and development of armyworms Effect. The evaluation indicators and their formulas used are as follows:

Mortality = number of dead insects / total number of tested insects × 100%

Adjusted mortality = (treatment mortality - control mortality) / (1 - control mortality) × 100%

Table 1 The effect of chicory ethyl acetate extract insect repellant on the growth and development of armyworm larvae (mg/head)

Initial Day 0 Day 1 3rd day Day 5 Day 7 Day 9 control 15.06±0.64a 20.13±1.88a 52.61±3.64a 81.03±2.42a 127.60±6.89a 194.82±10.54a insect repellent 15.13±0.82a 15.66±0.49b 17.86±3.78b 20.91±4.454b 38.97±5.22b 42.60±5.90b

It can be seen from Table 1 that the insect repellant effectively inhibited the growth and development of armyworm, and the body weight of the treatment group on the 9th day was only 20.5% of that of the control group, thus effectively prolonging the larval lifespan by 6 days (Table 2); finally The corrected mortality rate of Armyworm reached 71.30% (Table 2).

Table 2 The effect of chicory ethyl acetate extract insect repellant on armyworm pupae and survival

Larval duration (days) Pupation rate/% Mortality (including pupal death) % Adjusted mortality rate/% control 18.25±0.54a 89.57±2.68a 9.33±4.16a - insect repellent 24.90±1.29a 49.67±6.72b 71.80±6.41b 68.90

Claims (2)

1. a pest repellant that contains the witloof ethyl acetate extract is characterized in that, this pest repellant that makes is mixed by the raw material of following weight portion:

The witloof ethyl acetate extract: 0.30～0.34 part, the maize leaves powder: 2.0～2.2 parts, the wheat bran powder: 0.9～1.1 part, yeast: 0.8～0.9 part, agar: 0.40～0.45 part, B B-complex: 0.30～0.34 part, 22～27 parts in water;

Described witloof ethyl acetate extract prepares according to following method:

(1) will take witloof complete stool plant, root separates with cauline leaf, cleans up; With cutter root and cauline leaf are cut into long little breaking about 1cm, indoor drying in the shade places 35 ℃～40 ℃ of electric heating constant temperature air dry ovens then, and 24～48 hours to constant weight; Pulverize with plant sample cracker, cross 40 mesh sieves, obtain chicory plant dry powder;

(2) 1 part of above-mentioned chicory plant dry powder of weighing adds the ethyl acetate solvent of 10～11 weight portions, flood after 48 hours, with ultrasonic oscillation 0.5 hour, leave standstill 24 hours after, used ultrasonic oscillation once more 0.5 hour, after leave standstill filtration; Filtrating is evaporated under 75.5 ℃～77.5 ℃ temperature, is concentrated extraction, and solid matter is constantly extracted by solvent, until obtaining sticky paste, is the witloof ethyl acetate extract.

2. the described preparation method who contains the pest repellant of witloof ethyl acetate extract of claim 1 is characterized in that, comprises the following steps:

1) will take witloof complete stool plant, root separates with cauline leaf, cleans up; With cutter root and cauline leaf are cut into long little breaking about 1cm, indoor drying in the shade places 35 ℃～40 ℃ of electric heating constant temperature air dry ovens then, and 24～48 hours to constant weight; Pulverize with plant sample cracker, cross 40 mesh sieves, obtain chicory plant dry powder;

2) 1 part of above-mentioned chicory plant dry powder of weighing adds the ethyl acetate solvent of 10～11 weight portions, flood after 48 hours, with ultrasonic oscillation 0.5 hour, leave standstill 24 hours after, used ultrasonic oscillation once more 0.5 hour, after leave standstill filtration; Filtrating is evaporated under 75.5 ℃～77.5 ℃ temperature, is concentrated extraction, and solid matter is constantly extracted by solvent, until obtaining sticky paste, is the witloof ethyl acetate extract;

3) witloof ethyl acetate extract, maize leaves powder, wheat bran powder, yeast, agar, the B B-complex with formula ratio adds in the entry, mixes to get final product.

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