CN113841712A - Botanical vegetable insecticide and preparation method and application thereof - Google Patents
Botanical vegetable insecticide and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
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Abstract
The invention discloses a botanical vegetable insecticide, a preparation method and application thereof, wherein the botanical vegetable insecticide is prepared from the following raw materials in parts by weight: 30-35% of ginger extract, 20-25% of semen pharbitidis extract, 30-35% of green tomato extract, 0.3-0.5% of preservative and the balance of ultrapure water. The invention has the advantages of rich raw material resources, low cost, good insecticidal effect, continuous and stable drug effect and no residue on agricultural products. Meanwhile, the invention can obviously inhibit the activity of acetylcholinesterase of vegetable pests and improve the insecticidal effect. The active ingredients of the invention all belong to plant sources, have rich sources and no harm to people and livestock, and are a botanical pesticide suitable for producing pollution-free vegetables.
Description
Technical Field
The invention belongs to the technical field of vegetable insecticides, and particularly relates to a botanical vegetable insecticide and a preparation method and application thereof.
Background
China is a big agricultural country, vegetable production occupies an important proportion in agricultural production, and is one of essential foods in daily life of people, and the quality of vegetables is related to the quality of life of people. Currently, lepidopteran pests such as diamond back moths, beet armyworms, prodenia litura, olive armyworms and the like are the most common pests and diseases damaging the growth of vegetables. Lepidoptera pests usually take larvae as the most serious pests, and the larvae directly bite plants and tender parts thereof by chewing mouthparts, so that the growth of vegetables is seriously influenced, the vegetable appearance is poor, the quantity is reduced, and the economic value is reduced.
The main pesticide for preventing and controlling vegetable pests at present is chemical pesticide, which has good insecticidal effect but is difficult to degrade, so that pesticide residue in vegetables is easy to be long, and the health of eaters is affected. Moreover, chemical control causes pollution to the environment to a certain extent, and some pests have resistance to chemical pesticides, so that the difficulty and cost of chemical control are increased. Part of chemical pesticides can be accumulated in human bodies through circulation to generate accumulated toxicity, and great harm is brought to human health. Therefore, the plant source vegetable pesticide which is harmonious and friendly to the environment is urgently needed to be researched and developed.
The natural medicine of plant source has incomparable advantages of chemical medicine: the source is rich, the cost is low, the natural environment is easy to degrade, the pollution to the environment is small, the damage to the natural enemies of pests is small, the pests are not easy to generate antibodies, and the development and development of safe organic green ecological plants are helped. At present, scholars at home and abroad carry out series research on botanical vegetable insecticides or botanical pesticides, and the invention of application number CN201910633740.1 discloses a botanical pesticide composition and a preparation method and application thereof. The invention with the application number of CN201810374360.6 discloses a botanical vegetable insecticide, which has good killing effect on diamond back moth, orchid looper, beet armyworm and prodenia litura, the insecticidal rate in 72h reaches 100%, the field insecticidal rate in 72h reaches more than 70%, and the effect is obvious. The invention of application number CN201710520509.2 discloses a botanical pesticide and a preparation method and application thereof. Some botanical insecticides are available in the market at present, but are difficult to be widely popularized in practical application due to a plurality of reasons such as single component, poor prevention and control effect, short duration and the like.
Disclosure of Invention
One of the purposes of the invention is to provide an environment-friendly botanical vegetable pesticide aiming at the defects; the other purpose of the invention is to provide a preparation method of the botanical vegetable insecticide;
the invention also aims to provide the application of the botanical vegetable insecticide in preventing and controlling the larvae of the lepidoptera pests of the vegetables.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the invention firstly provides a botanical vegetable insecticide, which comprises the following components in parts by weight: 30-35% of ginger extract, 20-25% of semen pharbitidis extract, 30-35% of green tomato extract, 0.3-0.5% of preservative and the balance of ultrapure water;
in a preferred embodiment of the present invention, the preservative is protamine.
As a further improvement of the invention, the preparation method of the ginger extract comprises the following steps:
(1) cleaning: selecting fresh ginger without diseases, cleaning the fresh ginger with clear water and then airing the fresh ginger;
(2) crushing: pulverizing the ginger by a pulverizer until the particle size is less than 100 meshes;
(3) leaching: mixing the crushed powder according to a material-liquid ratio of 1: 10-20, leaching for 3-4 hours in a water bath kettle at 70-75 ℃, and stirring once every 30min during leaching;
(4) and (3) filtering: filtering the leaching solution with sterile gauze;
(5) concentrating the filtrate: mixing the filtrates, adding into rotary evaporator, and rotary evaporating at 70-75 deg.C to remove water to obtain extract;
(6) drying: drying in an oven at 40-45 deg.C, and storing in a drying bottle.
As a further improvement of the invention, the leaching liquor in the step (3) is ultrapure water, 95% ethanol solution or ethyl acetate.
As a further improvement of the invention, the preparation method of the pharbitis seed extract comprises the following steps:
(1) crushing: pulverizing semen Pharbitidis into powder with particle size less than 50 meshes by a pulverizer;
(2) leaching: mixing the crushed powder according to a material-liquid ratio of 1: putting the mixture into ultrapure water in a proportion of 15-30, and leaching for 24-28 hours in a water bath kettle at the temperature of 40-55 ℃;
(3) re-leaching: adding anhydrous ethanol into the mixed solution, wherein the adding amount of the anhydrous ethanol is 10-15 wt% of the added ultrapure water, and leaching by a heating reflux method;
(4) filtering the leaching solution under reduced pressure for 2-3 times, and concentrating the filtrate to obtain semen Pharbitidis extract.
As a further improvement of the present invention, the preparation method of the green tomato extract is as follows:
(1) cleaning: selecting fresh immature tomatoes without diseases, cleaning the tomatoes with clear water and then airing the tomatoes;
(2) slicing: after green tomatoes are sliced, crushing the green tomato slices by a crusher until the particle size is less than 100 meshes;
(3) leaching: mixing the crushed powder according to a material-liquid ratio of 1: putting the mixture into ultrapure water in a proportion of 10-20, and leaching for 10-12 hours at the temperature of 0 ℃;
(4) and (3) filtering: filtering the leaching solution with sterile gauze;
(5) concentrating the filtrate: mixing the filtrates, adding into a rotary evaporator, and rotary evaporating at 0-4 deg.C to remove water to obtain extract;
the second aspect of the invention provides a preparation method of the botanical vegetable pesticide, which comprises the following steps: dissolving rhizoma Zingiberis recens extract in ultrapure water, stirring, adding semen Pharbitidis extract and green fructus Lycopersici Esculenti extract, dissolving, adding protamine, and stirring.
In a third aspect, the invention provides the use of the botanical vegetable insecticide for controlling larvae of lepidopteran pests in vegetables.
As a further improvement of the invention, the use method is as follows: diluting the prepared botanical vegetable pesticide by water to 10-20 times, and spraying on vegetables. The botanical vegetable insecticide has the best effect when being applied to the early growth stage of vegetables.
The base sources and the efficacies of the various materials in the formulation are as follows:
ginger, also named as ginger root, Baicaoyun and the like, contains volatile oil, mainly including gingerol, shogaol, phellandrene, citral, linalool and the like; and contains a spicy component gingerol which can generate strong pungent smell, so that some pests dare not to invade the sprayed vegetables and have the functions of repelling and refusing to eat.
Semen Pharbitidis, also known as semen Pharbitidis, contains semen Pharbitidis glycoside, semen Pharbitidis acid A and Galla Turcica, and can stimulate intestines and stomach to cause emesis, food refusal and damage nervous system.
The solanine contained in the green tomatoes is a toxic alkalescent glucoside alkaloid, and the poisoning mechanism of the solanine is mainly to cause the death of pests by inhibiting the activity of cholinesterase.
The invention has the beneficial effects that:
(1) the material adopted by the invention is natural plant, has rich source and low cost, is easy to degrade in natural environment, is green and environment-friendly, and is nontoxic to human, bees and the like.
(2) The method has the advantages of simple process, good importance, higher product yield and high theoretical crude extract quality yield.
(3) Has high-efficiency insecticidal effect and quick response, and has the insecticidal effect equivalent to that of the commercial chemical medicament lufenuron (see table 2). Meanwhile, the invention can obviously inhibit the activity of acetylcholinesterase of vegetable pests and improve the insecticidal effect.
Drawings
FIG. 1 is a graph showing the effect of an insecticide on acetylcholinesterase (AChE) activity in 3 rd larvae of Plutella xylostella (Plutella xylostella).
Detailed Description
The invention will be further described with reference to specific examples, but the scope of the invention is not limited thereto:
example 1 a botanical vegetable insecticide composition was prepared as follows:
s1, the preparation method of the ginger extract comprises the following steps:
(1) cleaning: selecting fresh ginger without diseases, cleaning the fresh ginger with clear water and then airing the fresh ginger;
(2) crushing: pulverizing the ginger by a pulverizer until the particle size is less than 100 meshes;
(3) leaching: putting 100g of the crushed powder into 1500ml of ultrapure water, and leaching for 3 hours in a water bath kettle at 70 ℃, wherein the powder is stirred once every 30min during leaching;
(4) and (3) filtering: filtering the leaching solution with four layers of sterile gauze;
(5) concentrating the filtrate: mixing the filtrates, adding into a rotary evaporator, and rotary evaporating at 70 deg.C to remove water to obtain extract;
(6) drying: drying in an oven at 40 ℃, wherein the weight mass is (9.89 +/-0.0245) g, and the calculated mass yield is (9.89 +/-0.0245)%.
S2, the preparation method of the semen pharbitidis extract comprises the following steps:
(1) crushing: pulverizing semen Pharbitidis into powder with particle size less than 50 meshes by a pulverizer;
(2) leaching: taking the crushed powder according to the material-liquid ratio of 1: 15 into ultrapure water, leaching for 24 hours in a water bath kettle at 40 ℃;
(3) re-leaching: adding absolute ethyl alcohol into the mixed solution, wherein the adding amount of the absolute ethyl alcohol is 15 wt% of the added ultrapure water, and leaching by a heating reflux method;
(4) filtering the leaching solution under reduced pressure for 3 times, and concentrating the filtrate to obtain semen Pharbitidis leaching solution.
S3, the preparation method of the green tomato extract comprises the following steps:
(1) cleaning: selecting fresh immature tomatoes without diseases, cleaning the tomatoes with clear water and then airing the tomatoes;
(2) slicing: after green tomatoes are sliced, crushing the green tomato slices by a crusher until the particle size is less than 100 meshes;
(3) leaching: mixing the crushed powder according to a material-liquid ratio of 1: 10 is put into ultrapure water and leached for 10 hours at the temperature of 0 ℃;
(4) and (3) filtering: filtering the leaching solution with four layers of sterile gauze;
(5) concentrating the filtrate: mixing the filtrates, adding into a rotary evaporator, and rotary evaporating at 4 deg.C to remove water to obtain extract;
s4, a preparation method of the botanical vegetable insecticide comprises the following steps: firstly taking 15g of ginger extract, 10g of pharbitis seed extract, 15g of green tomato extract and 0.15g of protamine. Dissolving rhizoma Zingiberis recens extract in 9.85 g of ultrapure water, stirring, adding semen Pharbitidis extract and green tomato extract respectively, stirring, and adding protamine as antiseptic.
Example 2 a botanical vegetable insecticide composition was prepared as follows:
in this example S4, 17.5g of ginger extract, 12.5g of pharbitis seed extract, 17.5g of green tomato extract, 0.25g of protamine and 2.25 g of ultrapure water were first taken and mixed uniformly; the preparation method of ginger extract, the preparation method of pharbitis seed extract, the preparation method of green tomato extract, and other parameters and steps are the same as those in example 1.
Example 3 a botanical vegetable insecticide composition was prepared as follows:
in this embodiment S4, first, 16g of ginger extract, 11g of pharbitis seed extract, 16g of green tomato extract, 0.20g of protamine, and 6.8 g of ultrapure water are taken and mixed uniformly; the preparation method of ginger extract, the preparation method of pharbitis seed extract, the preparation method of green tomato extract, and other parameters and steps are the same as those in example 1.
Comparative example 1
This comparative example differs from example 1 only in that: the botanical vegetable insecticide of the comparative example was prepared without adding the pharbitis seed extract and the green tomato extract, the ginger extract was used in an amount of 40g, and the remaining components and preparation method were the same as in example 1.
Comparative example 2
This comparative example differs from example 1 only in that: the botanical vegetable insecticide of the comparative example was prepared without adding ginger extract and green tomato extract, the amount of the pharbitis seed extract was 40g, and the rest of the components and the preparation method were the same as those of example 1.
Comparative example 3
This comparative example differs from example 1 only in that: the botanical vegetable insecticide of the comparative example was prepared without adding ginger extract and pharbitis seed extract, the amount of the green tomato extract was 40g, and the remaining components and preparation method were the same as in example 1.
Comparative example 4
This comparative example differs from example 1 only in that: the botanical vegetable insecticide of the comparative example is not added with green tomato extract, the dosage of ginger extract is 24g, the dosage of pharbitis seed extract is 16g, and the other components and the preparation method are the same as the example 1.
Comparative example 5
This comparative example differs from example 1 only in that: the botanical vegetable insecticide of the comparative example is not added with the pharbitis seed extract, the dosage of the ginger extract is 20g, the dosage of the green tomato extract is 20g, and the other components and the preparation method are the same as the example 1.
Comparative example 6
This comparative example differs from example 1 only in that: the botanical vegetable insecticide of the comparative example is not added with ginger extract, the dosage of the pharbitis seed extract is 16g, the dosage of the green tomato extract is 24g, and the other components and the preparation method are the same as the example 1.
Examples of the experiments
1. Materials and methods
1.1 test Agents
The combination of botanical insecticides prepared in examples 1-3, the botanical vegetable insecticide composition prepared in comparative examples 1-6, a 5% aqueous lufenuron solution (g/kg).
1.2 preparation of the medicament
A1-3 agent: 200ml of the botanical vegetable insecticide composition prepared in examples 1 to 3 were diluted 10-fold and 20-fold respectively for use.
B1-6 agent: 200ml of the botanical vegetable insecticide compositions prepared in comparative examples 1 to 6 were diluted 10 times and 20 times, respectively, for use.
And C, medicament: 200ml of lufenuron water agent with the concentration of 5% (g/kg):
and (D) medicament: blank control (ultrapure water).
1.3 test insects
Diamondback moth (Plutella xylostella) 3-instar larvae.
1.4 methods of administration
The antifeedant and stomach toxicity of the botanical vegetable insecticide on plutella xylostella is measured by a small-leaflet disc addition method. Beating fresh cabbage leaves into leaf disks by using a circular punch (the diameter is 5mm), soaking the leaves in 1.2 parts of prepared medicament for 3s, naturally drying the leaves, putting the leaves into a culture dish (D is 10cm), putting 3-year-old larvae subjected to starvation treatment for 4h, and feeding the larvae in an insect feeding room. Each treatment was repeated 3 times, with 20 replicates. The blank was treated with ultrapure water. And measuring feeding areas of the larvae at 24 h, 48h and 72h after treatment, calculating the food refusal rate, and checking the death condition of the test insects.
The calculation formula is as follows:
the mortality rate is (dead insect number/total insect number) × 100%
2 results and analysis
The results are shown in Table 1. The results show that the insecticide prepared by the different modes has better control effect on 3-instar larvae of Plutella xylostella (Plutella xylostella) compared with the group C control group. Within 72h, the insecticidal rate of the A1, A2 and A3 insecticide diluted by 10 times is more than 90 percent, the insecticidal rate of the diluted solution 20 times is more than 86.67 percent, and the effect is obvious. Comparing the mortality rate of the group A with that of the group B, the insecticidal effect of any single extract or the combination of two extracts is far less than that of the three extract combination scheme, and the ginger extract, the pharbitis seed extract and the green tomato extract have obvious synergistic effect on insecticidal effect.
The three extracts are all plant sources, are rich in sources, low in cost, easy to degrade in natural environment, small in environmental pollution, less in damage to natural enemies of pests, and not easy to generate antibodies, and provide help for developing safe organic green ecological plants.
Table 1 insecticidal effect (mortality,%) of the botanical vegetable insecticide of the present invention
Meanwhile, the effect of the plant-derived vegetable insecticide of the present invention on acetylcholinesterase (AChE) activity of 3-instar larvae of Plutella xylostella (Plutella xylostella) was also determined. The results are shown in fig. 1, and the 11 bar groups in each time-corresponding result in the graph are respectively a group D, a group C, a group a1, a group a2, A3, a group B1, a group B2, a group B3, a group B4, a group B5 and a group B6 from left to right.
The results show that the agents of the invention have an effect on the activity of larval acetylcholinesterase (AChE) compared to the control group D, with the activity of group a1 being 61% of control D, the activity of group a2 being 45% of control D and the activity of group A3 being 50% of control D within 48 h; within 72h, the activity of the group A1 is 40% of that of the control group D, the activity of the group A2 is 37% of that of the control group D, and the activity of the group A3 is 45% of that of the control group D, so that the inhibition effect on the larvae AChE is remarkable, the inhibition effect is obviously stronger than that of the group C (lufenuron aqua), and the insecticidal effect of the pesticide can be remarkably improved. Meanwhile, the three extract combination scheme of the present invention exhibited a synergistic inhibitory effect on the acetylcholinesterase activity of larvae as compared to group B.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (10)
1. The botanical vegetable pesticide is characterized by being prepared from the following raw materials in parts by weight: 30-35% of ginger extract, 20-25% of semen pharbitidis extract, 30-35% of green tomato extract, 0.3-0.5% of preservative and the balance of ultrapure water.
2. The botanical vegetable insecticide as claimed in claim 1, wherein said green tomato extract is an immature tomato extract.
3. The botanical vegetable insecticide as claimed in claim 1, wherein said preservative is protamine.
4. The botanical vegetable insecticide as claimed in claim 1, wherein said ginger extract is prepared by the following steps:
(1) cleaning: selecting fresh ginger without diseases, cleaning the fresh ginger with clear water and then airing the fresh ginger;
(2) crushing: pulverizing the ginger until the particle size is less than 100 meshes;
(3) leaching: mixing the crushed powder according to a material-liquid ratio of 1: 10-20, leaching for 3-4 hours in a water bath kettle at 70-75 ℃, and stirring once every 30min during leaching;
(4) and (3) filtering: filtering the leaching solution with sterile gauze;
(5) concentrating the filtrate: mixing the filtrates, adding into rotary evaporator, and rotary evaporating at 70-75 deg.C to remove water to obtain extract;
(6) drying: drying in an oven at 40-45 deg.C, and storing in a drying bottle.
5. The botanical vegetable insecticide as claimed in claim 4, wherein said leaching solution is ultrapure water, 95% ethanol solution or ethyl acetate.
6. The botanical vegetable insecticide as claimed in claim 1, wherein said pharbitis seed extract is prepared by the following steps:
(1) crushing: beating the black pharbitis into powder with the granularity of less than 50 meshes;
(2) leaching: mixing the crushed powder according to a material-liquid ratio of 1: 15 to 30 percent of the raw materials are put into ultrapure water and leached for 24 to 28 hours in a water bath kettle at the temperature of between 40 and 55 ℃;
(3) re-leaching: adding anhydrous ethanol into the mixed solution, wherein the adding amount of the anhydrous ethanol is 10-15 wt% of the added ultrapure water, and leaching by a heating reflux method;
(4) filtering the leaching solution under reduced pressure for 2-3 times, and concentrating the filtrate to obtain semen Pharbitidis extract.
7. The botanical vegetable insecticide as claimed in claim 1, wherein said green tomato extract is prepared by the following method:
(1) cleaning: selecting fresh immature tomatoes without diseases, cleaning the tomatoes with clear water and then airing the tomatoes;
(2) slicing: after green tomatoes are sliced, crushing the green tomato slices to a particle size of less than 100 meshes;
(3) leaching: mixing the crushed powder according to a material-liquid ratio of 1: putting the mixture into ultrapure water in a proportion of 10 to 20, and leaching for 10 to 12 hours at the temperature of 0 ℃;
(4) and (3) filtering: filtering the leaching solution with sterile gauze;
(5) concentrating the filtrate: mixing the filtrates, adding into rotary evaporator, and rotary evaporating at 0-4 deg.C to remove water to obtain extract.
8. A method for preparing a plant-derived vegetable pesticide according to any one of claims 1 to 7, comprising the steps of: dissolving rhizoma Zingiberis recens extract in ultrapure water, stirring, adding semen Pharbitidis extract and green fructus Lycopersici Esculenti extract, dissolving, adding protamine, and stirring.
9. Use of a botanical vegetable insecticide as claimed in claim 1 for controlling larvae of a vegetable lepidopteran pest.
10. The use according to claim 9, characterized in that the method of use is as follows: diluting the prepared botanical vegetable pesticide by water to 10-20 times, and spraying on vegetables.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115005241A (en) * | 2022-06-30 | 2022-09-06 | 黑龙江省农业科学院齐齐哈尔分院 | Plant-derived pesticide, preparation method and application thereof |
| CN115005240A (en) * | 2022-05-31 | 2022-09-06 | 中国计量大学 | Plant source and microorganism source compound insecticide and preparation method and application thereof |
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