CN101785485B - Environment-friendly insecticide based on chicory ethanol extract and preparation method thereof - Google Patents
Environment-friendly insecticide based on chicory ethanol extract and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an environment-friendly insectifuge based on chicory ethanol extract and a preparation method thereof. The environment-friendly insectifuge based on chicory ethanol extract is prepared by mixing the following components in parts by weight: 20-25 parts of water, 0.25-0.30 parts of chicory ethanol extract, 1.8-2.1 parts of corn leaf powder, 0.8-1 part of wheat bran powder, 0.7-0.9 parts of yeast, 0.4-0.5 parts of agar, 0.28-0.32 parts of compound vitamin and 0.75-0.865 parts of antibacterial agent. The environment-friendly insectifuge can protect the ecology and can not pollute the environment in the processes of production and application, and has larger market prospects in agricultural production practice.
Description
Technical field
The invention belongs to plant resource environment friendly agricultural technical field, relate to a kind of green pest repellant based on the witloof ethanol extract and preparation method thereof.
Background technology
Existing various desinsections and the expelling parasite agricultural chemicals that is used on vegetables, woods fruit, tealeaves and the crops all has to a certain degree toxicity and side effect.Its toxic component can cause soil, water source and biological pollution to some extent.In the today of advocating green agriculture, substitute traditional agricultural chemicals to reduce environmental pollution with pure natural plant product pest repellant, be the task of top priority, imperative.
In the exploitation of plant resource environment friendly agricultural, feverfew is one of most important plant classification.Witloof (Cichorium intybus L.) is a composite family Cichorium herbaceos perennial, originates in Europe, West Asia, the Central Asia and North America, and all there is distribution in areas such as NORTHWEST CHINA, North China and northeast.Witloof is of high nutritive value, disease and insect resistance and high yield, and regeneration capacity is strong, is a kind of plant resources that potentiality to be exploited is arranged very much.
It is more to contain the chemical composition type in the natural products of chicory extract; Mainly contain sesquiterpene lactone class, Diterpenes, sesquiterpenoids, monoterpenes, flavonoids, Coumarins and sterols etc., structure type that they are changeable and steric configuration make this plant present wide biological activity.With the witloof is the natural products that raw material extracts, and is widely used as feed green additive, wholefood batching, pharmaceutical carrier and vaccine assistant agent etc.
Summary of the invention
The problem that the present invention solves is to provide a kind of green pest repellant based on the witloof ethanol extract and preparation method thereof, the production of this green pest repellant and use this pest repellant both to protect ecology, and non-environmental-pollution has than larger market prospect in agricultural production practice again.
The present invention realizes through following technical scheme:
A kind of green pest repellant based on the witloof ethanol extract is mixed by the component of following mass parts:
20～25 parts water, 0.25～0.30 part witloof ethanol extract, 1.8～2.1 parts maize leaves powder; 0.8～1 part wheat bran powder; 0.7～0.9 part dusty yeast, 0.4～0.5 part agar, the antibacterial agent of 0.28～0.32 part B B-complex and 0.75～0.865 part.
Behind the ethanol lixiviate 48h of the dry powder that described witloof ethanol extract is cichory root and cauline leaf with 70% volume fraction, extract 2 times with ultrasonic oscillation, each 0.5h, separation and Extraction liquid then is with its paste at 77～79 ℃ of concentrated gained.
Described B B-complex comprises vitamin A, vitamin B1, vitamin B2, vitamin B6, cobalamin, vitamin C, vitamin D, vitamin E, folic acid, calcium carbonate, glucose, malic acid and citric acid.
Described antibacterial agent is 0.7～0.8 part a nipalgin, the sorbic acid of 0.025～0.035 part gallic acid and 0.025～0.03 part.
A kind of preparation method of the green pest repellant based on the witloof ethanol extract may further comprise the steps:
1) segment that respectively root and the cauline leaf of witloof is cut into 1～2cm, indoor drying in the shade is dried to constant weight at 35～40 ℃ then, again with its pulverizing and cross 40 mesh sieves, obtains chicory plant dry powder;
2) ethanol of 70% volume fraction of 6.0～6.5 parts of mass parts of per 1 part of mass parts chicory plant dry powder adding is as solvent; Under the normal temperature behind the lixiviate 48h; Under ultrasonic oscillation, after extract 0.5h, extracting back 24h first, under ultrasonic oscillation, extract 0.5h once more; Leave standstill the back and filter, separate obtaining extract;
3) extract is carried out reduction vaporization at 77～79 ℃, be concentrated into paste, obtain the witloof ethanol extract;
4), in 0.25～0.30 part witloof ethanol extract, add 20～25 parts water, and then add 1.8～2.1 parts maize leaves powder respectively in mass parts; 0.8～1 part wheat bran powder, 0.7～0.9 part yeast, 0.4～0.5 part agar; 0.28～0.32 part B B-complex; 0.7～0.8 part nipalgin, the sorbic acid of 0.025～0.035 part gallic acid and 0.025～0.03 part fully stirs the back mixing.
Described ultrasonic is to be produced by the ultrasonic generator, and the parameter of ultrasonic generator is set at: power is 200～250W, and temperature is 55～60 ℃.
Compared with prior art, the present invention has following beneficial technical effects:
Green pest repellant based on the witloof ethanol extract provided by the invention, its main effective component is the witloof ethanol extract, derives from the natural plant extract, non-environmental-pollution; Maize leaves powder, dusty yeast, wheat bran powder, agar and B B-complex are the carrier of effective component, simultaneously the bait of double as insect.
Green pest repellant based on the witloof ethanol extract provided by the invention; Insect had good causing death and growth inhibitory effect: the mythimna separata lethal effect is surpassed 50%; And the mythimna separata of all the other survivals has then been suppressed growth effectively, and its growth period has been extended 6 days; Make mythimna separata stagger farming season and reduce harm, do not destroy biological chain simultaneously and protect ecology crop.
Green pest repellant based on the witloof ethanol extract provided by the invention, with low cost, when effective inhibition insect is to the harm of crop, do not destroy biological chain again, environmental protection has than larger market prospect in agricultural production practice.
Embodiment
Below in conjunction with concrete embodiment the present invention is done detailed explanation, said is to explanation of the present invention rather than qualification.
The present invention is prepared the related raw material of green pest repellant does following explanation:
After the witloof ethanol extract is the ethanol lixiviate 48h of dry powder with 70% volume fraction of cichory root and cauline leaf; Extract twice with ultrasonic oscillation, interval 24h extracts 0.5h at every turn between the extracted twice; Separation and Extraction liquid then is with its paste at 77～79 ℃ of concentrated gained;
The maize leaves powder is with the abundant crushed after being dried of maize leaf and crosses 40 mesh sieves;
Dusty yeast, wheat bran powder, agar, B B-complex all can be purchased acquisition;
" B B-complex " that the used B B-complex of the present invention specifically adopts Jining Heng Kang bio tech ltd to produce, main component has: vitamin A, vitamin B1, vitamin B2, vitamin B6, cobalamin, vitamin C, vitamin D, vitamin E, folic acid, calcium carbonate, glucose, malic acid and citric acid.
The chemical name of nipalgin is a methyl hydroxybenzoate, the chemical name 3,4 of gallic acid, and 5-trihydroxybenzoic acid, the chemical name of sorbic acid are 2, the 4-hexadienoic acid; All can be purchased acquisition.
In the middle of the related component of the green pest repellant of the present invention, water is solvent, and the witloof ethanol extract is main desinsection and resist the active component that insect grows, and is the auxiliary activity composition as nipalgin, gallic acid and the sorbic acid of antibacterial agent; Maize leaves powder, wheat bran powder, yeast, agar and B B-complex are nutrient matrixes, are the carrier of active component, and double as pest food (bait) again is not in order to make the insect food refusal.
Embodiment 1
Green pest repellant based on the witloof ethanol extract prepares through following steps:
1) takes witloof complete stool plant, root and cauline leaf cutting are separated, and clean up; With the segment that cutter cuts into 1～2cm with root and the cauline leaf of witloof respectively, indoor drying in the shade places the electric heating constant temperature air dry oven then,, it pulverized with cracker again and crosses 40 mesh sieves to constant weight at 35 ℃ of dry 24h, obtains chicory plant dry powder;
2) ethanol of 70% volume fraction that adds 6.0 parts of mass parts by per 1 part of mass parts chicory plant dry powder is as solvent, and behind the lixiviate 48h, (power setting of ultrasonic generator is 200W at ultrasonic under the normal temperature; Temperature is set at 55 ℃; And add entry) concussion extracts 0.5h down, extract back 24h first after, extraction 0.5h under ultrasonic oscillation once more; Leave standstill the back and filter, separate obtaining extract;
3) extract is carried out reduction vaporization at 77 ℃, solid matter is constantly extracted by solvent, concentrate and be extracted to sticky paste, obtain the witloof ethanol extract; This process is according to the efficient of the amount of extract what and evaporation equipment, and needs are several to tens hours;
4), in 0.25 part witloof ethanol extract, add 20 parts water, and then add 1.8 parts maize leaves powder respectively in mass parts; 0.8 the wheat bran powder of part, 0.7 part yeast, 0.4 part agar; 0.28 the B B-complex of part; 0.7 part nipalgin, the sorbic acid of 0.025 part gallic acid and 0.025 part fully stirs the back mixing.
Embodiment 2
Green pest repellant based on the witloof ethanol extract prepares through following steps:
1) takes witloof complete stool plant, root and cauline leaf cutting are separated, and clean up; With the segment that cutter cuts into 1～2cm with root and the cauline leaf of witloof respectively, indoor drying in the shade places the electric heating constant temperature air dry oven then,, it pulverized with cracker again and crosses 40 mesh sieves to constant weight at 40 ℃ of dry 24h, obtains chicory plant dry powder;
2) ethanol of 70% volume fraction that adds 6.5 parts of mass parts by per 1 part of mass parts chicory plant dry powder is as solvent, and behind the lixiviate 48h, (power setting of ultrasonic generator is 250W at ultrasonic under the normal temperature; Temperature is set at 60 ℃; And add entry) concussion extracts 0.5h down, extract back 24h first after, extraction 0.5h under ultrasonic oscillation once more; Leave standstill the back and filter, separate obtaining extract;
3) extract is carried out reduction vaporization at 79 ℃, solid matter is constantly extracted by solvent, concentrate and be extracted to sticky paste, obtain the witloof ethanol extract;
4), in 0.30 part witloof ethanol extract, add 25 parts water, and then add 2.1 parts maize leaves powder respectively in mass parts; 1 part wheat bran powder, 0.9 part yeast, 0.5 part agar; 0.32 the B B-complex of part; 0.8 part nipalgin, the sorbic acid of 0.035 part gallic acid and 0.03 part fully stirs the back mixing.
Embodiment 3
Green pest repellant based on the witloof ethanol extract prepares through following steps:
1) takes witloof complete stool plant, root and cauline leaf cutting are separated, and clean up; With the segment that cutter cuts into 1～2cm with root and the cauline leaf of witloof respectively, indoor drying in the shade places the electric heating constant temperature air dry oven then,, it pulverized with cracker again and crosses 40 mesh sieves to constant weight at 38 ℃ of dry 24h, obtains chicory plant dry powder;
2) ethanol of 70% volume fraction that adds 6.2 parts of mass parts by per 1 part of mass parts chicory plant dry powder is as solvent, and behind the lixiviate 48h, (power setting of ultrasonic generator is 220W at ultrasonic under the normal temperature; Temperature is set at 58 ℃; And add entry) concussion extracts 0.5h down, extract back 24h first after, extraction 0.5h under ultrasonic oscillation once more; Leave standstill the back and filter, separate obtaining extract;
3) extract is carried out reduction vaporization at 78 ℃, solid matter is constantly extracted by solvent, concentrate and be extracted to sticky paste, obtain the witloof ethanol extract;
4), in 0.26 part witloof ethanol extract, add 22 parts water, and then add 1.9 parts maize leaves powder respectively in mass parts; 0.9 the wheat bran powder of part, 0.8 part yeast, 0.45 part agar; 0.30 the B B-complex of part; 0.75 part nipalgin, the sorbic acid of 0.030 part gallic acid and 0.028 part fully stirs the back mixing.
Embodiment 4
Green pest repellant based on the witloof ethanol extract prepares through following steps:
1) takes witloof complete stool plant, root and cauline leaf cutting are separated, and clean up; With the segment that cutter cuts into 1～2cm with root and the cauline leaf of witloof respectively, indoor drying in the shade places the electric heating constant temperature air dry oven then,, it pulverized with cracker again and crosses 40 mesh sieves to constant weight at 40 ℃ of dry 24h, obtains chicory plant dry powder;
2) ethanol of 70% volume fraction that adds 6.3 parts of mass parts by per 1 part of mass parts chicory plant dry powder is as solvent, and behind the lixiviate 48h, (power setting of ultrasonic generator is 200W at ultrasonic under the normal temperature; Temperature is set at 58 ℃; And add entry) concussion extracts 0.5h down, extract back 24h first after, extraction 0.5h under ultrasonic oscillation once more; Leave standstill the back and filter, separate obtaining extract;
3) extract is carried out reduction vaporization at 78 ℃, solid matter is constantly extracted by solvent, concentrate and be extracted to sticky paste, obtain the witloof ethanol extract;
4), in 0.30 part witloof ethanol extract, add 21 parts water, and then add 2.0 parts maize leaves powder respectively in mass parts; 1 part wheat bran powder, 0.9 part yeast, 0.4 part agar; 0.30 the B B-complex of part; 0.7 part nipalgin, the sorbic acid of 0.03 part gallic acid and 0.03 part fully stirs the back mixing.
Embodiment 5
Green pest repellant based on the witloof ethanol extract prepares through following steps:
1) takes witloof complete stool plant, root and cauline leaf cutting are separated, and clean up; With the segment that cutter cuts into 1～2cm with root and the cauline leaf of witloof respectively, indoor drying in the shade places the electric heating constant temperature air dry oven then,, it pulverized with cracker again and crosses 40 mesh sieves to constant weight at 40 ℃ of dry 48h, obtains chicory plant dry powder;
2) ethanol of 70% volume fraction that adds 6.0 parts of mass parts by per 1 part of mass parts chicory plant dry powder is as solvent, and behind the lixiviate 48h, (power setting of ultrasonic generator is 200W at ultrasonic under the normal temperature; Temperature is set at 60 ℃; And add entry) concussion extracts 0.5h down, extract back 24h first after, extraction 0.5h under ultrasonic oscillation once more; Leave standstill the back and filter, separate obtaining extract;
3) extract is carried out reduction vaporization at 78 ℃, solid matter is constantly extracted by solvent, concentrate and be extracted to sticky paste, obtain the witloof ethanol extract;
4), in 0.28 part witloof ethanol extract, add 22 parts water, and then add 1.8 parts maize leaves powder respectively in mass parts; 0.9 the wheat bran powder of part, 0.8 part yeast, 0.45 part agar; 0.28 the B B-complex of part; 0.76 part nipalgin, the sorbic acid of 0.026 part gallic acid and 0.028 part fully stirs the back mixing.
Based on the green pest repellant of witloof ethanol extract compliance test result to mythimna separata (Mythimna separata Walker)
The employing weight method of feeding is measured based on the green pest repellant of the witloof ethanol extract inhibition effect to mythimna separata.With mythimna separata artificial feed (prescription adopts document: Wang Yannian work, insect artificial diet's handbook, 1984, Shanghai science tech publishing house publishes) feed grow neat, known body weight mythimna separata as control group; With based on the green pest repellant of witloof ethanol extract as experimental group, put into 1～2g (mistake capacity) artificial feed for respectively control group and experimental group (green pest repellant is fed) and green pest repellant is fed at every turn;
Connect 1 of worm of 4 ages in days examinations with every ware and in climatic cabinate, raise, climatic cabinate is set to 25 ℃ of illumination every day 8h and temperature, 15 ℃ of dark 16h and temperature; 30 of every processing repeat 3 times;
Processed group is replaced by artificial feed and is raised respectively in initial the 0th day to the 3rd day pest repellant of feeding after the 4th day; The control group artificial feed of feeding all the time;
Continue up to the examination worm and pupate, the getting food, grow and the life and death reaction of observed in detail examination worm, and the examination of weighing regularly polypide is heavy, adds up pupation rate and lethality, the inhibition effect that the multifactorial evaluation pest repellant grows to mythimna separata.
Used evaluation index and formula thereof are following:
Lethality=dead borer population/confession examination total borer population * 100%
Corrected mortality=(handling lethality-contrast lethality)/(1-contrasts lethality) * 100%
The testing result of armyworm larvae body weight as shown in table 1; Green pest repellant provided by the invention can suppress growing of mythimna separata effectively: cultivating the 9th day; Processed group mythimna separata body weight has only 20.5% of control group, has obviously suppressed growing of mythimna separata;
As shown in table 2 to mythimna separata lethal effect, the testing result of larva growth stage, green pest repellant provided by the invention surpasses 50% to the mythimna separata lethal effect, has reached 53.46%; And the mythimna separata of all the other survivals has then been suppressed its growth effectively, and larva is gone through the phase and has been extended 6 days.
Comprehensive Assessment, green pest repellant provided by the invention can effectively suppress growing of mythimna separata, and the lethality rate of mythimna separata has been reached 53.46%, and the mythimna separata of all the other survivals, and larva is gone through the phase and has been extended 6 days, and body weight obviously reduces.
Verification the verifying results shows that the green pest repellant based on the witloof ethanol extract provided by the invention is a kind of effective pest repellant, and because it derives from natural plant extract, non-environmental-pollution, environmental protection.
The green pest repellant of table 1 is to the armyworm larvae effect (mg/ head) that (body weight) suppress of growing
| Initial the 0th day | The 1st day | The 3rd day | The 5th day | The 7th day | The 9th day | |
| Contrast | 15.06±0.64a | 20.13±1.88a | 52.61±3.64a | 81.03±2.42a | 127.60±6.89a | 194.82±10.5 4a |
| Pest repellant | 15.01±0.85a | 15.65±2.25b | 18.09±0.95b | 19.49±1.454b | 36.53±4.28b | 39.96±6.75b |
The green pest repellant of table 2 is to the effect of mythimna separata pupa and survival
| Larva go through the phase (my god) | Pupa rate/% | Lethality (comprising that pupa is dead) % | Corrected mortality/% | |
| Contrast | 18.25±0.54a | 89.57±2.68a | 9.33±4.16a | - |
| Pest repellant | 24.50±1.01a | 46.67±3.35b | 57.10±5.54b | 53.46 |
Claims (5)
1. the green pest repellant based on the witloof ethanol extract is characterized in that, is mixed by the component of following mass parts: 20～25 parts water; 0.25～0.30 part witloof ethanol extract; 1.8～2.1 parts maize leaves powder, 0.8～1 part wheat bran powder, 0.7～0.9 part dusty yeast; 0.4～0.5 part agar, the antibacterial agent of 0.28～0.32 part B B-complex and 0.75～0.865 part;
Behind the ethanol lixiviate 48h of the dry powder that described witloof ethanol extract is cichory root and cauline leaf with 70% volume fraction; With separated 24h between the ultrasonic oscillation extraction twice, twice, extract 0.5h at every turn; Separation and Extraction liquid then is with its paste at 77～79 ℃ of concentrated gained.
2. the green pest repellant based on the witloof ethanol extract as claimed in claim 1; It is characterized in that B B-complex comprises vitamin A, vitamin B1, vitamin B2, vitamin B6, cobalamin, vitamin C, vitamin D, vitamin E, folic acid, calcium carbonate, glucose, malic acid and citric acid.
3. the green pest repellant based on the witloof ethanol extract as claimed in claim 1 is characterized in that described antibacterial agent is 0.7～0.8 part a nipalgin, the sorbic acid of 0.025～0.035 part gallic acid and 0.025～0.03 part.
4. the preparation method based on the green pest repellant of witloof ethanol extract is characterized in that, may further comprise the steps:
1) segment that respectively root and the cauline leaf of witloof is cut into 1～2cm, indoor drying in the shade is dried to constant weight at 35～40 ℃ then, again with its pulverizing and cross 40 mesh sieves, obtains chicory plant dry powder;
2) ethanol of 70% volume fraction of 6.0～6.5 parts of mass parts of per 1 part of mass parts chicory plant dry powder adding is as solvent; Under the normal temperature behind the lixiviate 48h; Under ultrasonic oscillation, extract 0.5h, behind the 24h, under ultrasonic oscillation, extract 0.5h once more after extracting first; Leave standstill the back and filter, separate obtaining extract;
3) extract is carried out reduction vaporization at 77～79 ℃, be concentrated into paste, obtain the witloof ethanol extract;
4), in 0.25～0.30 part witloof ethanol extract, add 20～25 parts water, and then add 1.8～2.1 parts maize leaves powder respectively in mass parts; 0.8～1 part wheat bran powder, 0.7～0.9 part yeast, 0.4～0.5 part agar; 0.28～0.32 part B B-complex; 0.7～0.8 part nipalgin, the sorbic acid of 0.025～0.035 part gallic acid and 0.025～0.03 part fully stirs the back mixing.
5. the preparation method of the green pest repellant based on the witloof ethanol extract as claimed in claim 4; It is characterized in that; Described ultrasonic is to be produced by the ultrasonic generator, and the parameter of ultrasonic generator is set at: power is 200～250W, and temperature is 55～60 ℃.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101012284A (en) * | 2007-01-09 | 2007-08-08 | 鞍山中兴医药集团有限公司 | Preparing process for extracting inulin from chicory root |
| CN101167776A (en) * | 2007-11-09 | 2008-04-30 | 中国科学院新疆理化技术研究所 | Chicory seed effective part and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101012284A (en) * | 2007-01-09 | 2007-08-08 | 鞍山中兴医药集团有限公司 | Preparing process for extracting inulin from chicory root |
| CN101167776A (en) * | 2007-11-09 | 2008-04-30 | 中国科学院新疆理化技术研究所 | Chicory seed effective part and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 白雪峰等.菊苣叶中生物活性物质的提取及其抑菌活性初测.《现代农业科技》.2009,(第15期),76-77. * |
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