CN102150551B - Method for grafting, closing and rejuvenating superior plus tree of empress tree - Google Patents
Method for grafting, closing and rejuvenating superior plus tree of empress tree Download PDFInfo
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- CN102150551B CN102150551B CN2011100320222A CN201110032022A CN102150551B CN 102150551 B CN102150551 B CN 102150551B CN 2011100320222 A CN2011100320222 A CN 2011100320222A CN 201110032022 A CN201110032022 A CN 201110032022A CN 102150551 B CN102150551 B CN 102150551B
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Abstract
The invention provides a method for rejuvenating a superior plus tree of empress tree, in particular to a method for grafting, closing and rejuvenating a superior plus tree of empress tree, which is a method for rejuvenating an old superior plus tree of empress tree. The method comprises the steps of: grafting and rejuvenating a superior plus tree of empress tree material; processing rejuvenation with phytokinin; carrying out secondary grafting rejuvenating in summer; processing with growth hormone; closing and rooting; and burying the root and breeding. The invention overcomes the defects of difficult collection and low burying germination rate of root sections of the foxglove superior plug tree, and effectively realizes rejuvenation of the foxglove superior plug tree; meanwhile, the rejuvenation period of the empress superior plug tree is shortened, the rejuvenation efficiency is improved, the progress of breeding the foxglove tree is forcefully promoted, the guarantee is provided for smoothly carrying out later-stage excellent clonal test and high-quality seed popularization works of the empress breeding. According to the invention, rejuvenation success rate and rejuvenation efficiency of the foxglove superior plus tree can be improved, and remarkable rejuvenation effect on superior plus trees such as paulownia tomentosa, paulownia fargesii, paulownia kawakamii, paulownia australis and the like is obtained.
Description
Technical field:
The present invention relates to the childrenization method of a kind of paulownia select tree; Particularly a kind of method of paulownia select tree grafting grave mound childrenization; Be the childrenization method to aged paulownia select tree, it is applicable to the childrenization to the serious select tree material of paulownia maturing effect, thereby has improved the asexual multiplication ability of paulownia select trees.
Background technology:
Paulownia are that the most important speed of China is given birth to one of high-quality commerical tree species and ecological protection seeds, are bringing into play irreplaceable effect aspect construction of Chinese commodity woods and the ecological environmental protection always.China also is the country that paulownia germ plasm resource is the abundantest in the world, distribution is the widest, and only just there are nine kind of two mutation and numerous variation type in the continent.Yet because nature and various reasons such as artificial, China's paulownia germ plasm resource reduces day by day, some precious paulownia resource going to wreck property destructions or endangered particularly, and the survival and development of the peculiar precious resources of China in this serious threat.It is extremely urgent to save, protect and fully utilize paulownia germ plasm resource, and collecting good paulownia germ plasm resource and carrying out vegetative propagation is the requisite measure of saving and protect paulownia germ plasm resource.
At present, the method for burying root propagation is mainly adopted in the breeding of paulownia.Yet, receiving the restriction of growing environment condition, most paulownia select tree root segment collections are difficulty very; Though the part select tree can collect root segment, germination rate is also very low, therefore, is difficult to bury root propagation.The collection of paulownia select tree material is mainly taked to collect the select tree branch and is carried out.Receive the influence of maturation effect; Seriously weak on the select tree branch physiology of being gathered; Asexual multiplication ability sharply reduces, and adopts conventional cuttage and tissue culture all to be difficult to succeed, and adopts conventional grafting method; Can not obtain the root system of select tree material self again, make later choiceness measure and afforestation is buried root in producing and grown seedlings and can't normally carry out.Therefore, carry out the childrenization, obtain to have the root segment material of strong asexual multiplication ability, with aged select tree material childrenization and to carry out vegetative propagation be the major issue in the breeding research work aged select tree material.
Summary of the invention:
In sum; In order to solve the childrenization technical problem; The present invention develops a kind of method of paulownia select tree grafting grave mound childrenization, and this method is that paulownia select tree material is carried out the grafting rejuvenation, and then carry out the childrenization through growth regulator processing and grave mound root induction; The childrenization efficient of the serious paulownia select tree material of maturing effect is improved greatly, for the later stage choiceness of paulownia breeding measure and improved variety popularization work guarantee is provided smoothly.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of method of paulownia select tree grafting grave mound childrenization, this method may further comprise the steps:
The first step, grafting rejuvenation: the fortune paulownia of collecting in autumn, winter or early spring or royal paulownia or river paulownia or Formosan paulownia (Cortex seu Radix Paulowniae kawakamii) select tree branch are carried out grafting mid-September or late March; Grafting method adopts bud grafting to carry out; Grafting height is 6～8cm overhead, and stock adopts 9501 clonal tissue culture seedlings;
Second step, the basic element of cell division are handled the childrenization: early April, compound concentration was that the basic element of cell division 6-benzyladenine solution of 1500～2000mg/L carries out foliage-spray to grafting to late May, whenever at a distance from spray in 7～10 days once, sprayed continuously 3～5 times;
The 3rd the step, summer the secondary grafting childrenization: mid or late June, gathering first graft material is that the grafting second time is carried out in scion, and grafting height 4～6cm, stock adopt 9501 clone pile seedlings;
The 4th step, growth hormone are handled: when treating that scion is sprouted to 10～25cm, with growth hormone grafting coppice shoot base portion is handled;
The 5th step, grave mound are taken root: the branch base portion that scion is sprouted carries out the grave mound processing, and the grave mound height is grafting mouth at least 6～8cm, keeps ground moistening during the grave mound;
The 6th goes on foot, buries root propagation: in late March, second is gathered paulownia cuttage seeding root segment to early April, and selecting root directly is 0.5～1.5cm; The root segment of length 10～20cm buries root propagation, strengthens water and fertilizer management; Prevention and elimination of disease and pests in time, and 7～mid-August in the ridging of nursery stock base portion once.
Further, the growth hormone that described the 4th one-step growth is plain in handling is ABT1 or methyl, and processing method is that the growth hormone of 1000～2000mg/L is pasted on the scion base portion that the grafting mouth is sprouted for using blotting paper to dip in to get concentration.
Further, to take root be to adopt repeatedly grave mound method to described the 5th step grave mound, when scion is sprouted to 5～8cm, begins grave mound, and along with the growth of scion, grave mound 2～3 times is to 6～8cm more than the grafting mouth, and keeps ground moistening.
Good effect of the present invention is:
1, the present invention carries out the grafting rejuvenation with paulownia select tree material, and then carry out the childrenization through growth regulator processing and grave mound root induction.It improves the childrenization efficient of paulownia select tree material greatly, for the later stage choiceness of paulownia breeding measure and improved variety popularization work guarantee is provided smoothly.
2, it is serious to The present invention be directed to paulownia select tree material maturing effect; The outstanding problem that asexual multiplication ability is low; Work out a kind of paulownia select tree childrenization method; Through enforcement of the present invention, can overcome paulownia select tree vegetative propagation maturing effect problem effectively, for paulownia select tree childrenization provides a quick approach.
3, the present invention can improve the success rate and the childrenization efficient of the childrenization of fortune paulownia select tree, to royal paulownia, and the river paulownia, Formosan paulownia (Cortex seu Radix Paulowniae kawakamii), the childrenization effect of select trees such as paulownia australis is particularly evident.
Embodiment:
Below in conjunction with embodiment the present invention is done further detailed description.
Embodiment 1:(material is the fortune paulownia select tree)
A kind of method of paulownia select tree grafting grave mound childrenization, this method may further comprise the steps:
The first step, grafting rejuvenation: mid-September, the fortune paulownia select tree branch that will collect autumn carries out grafting, and grafting method adopts bud grafting to carry out, and grafting height is 6～8cm overhead, and stock adopts 9501 clonal tissue culture seedlings;
Second step, the basic element of cell division are handled childrenization: respectively at April 10,17 days and 25 days, compound concentration was the basic element of cell division 6-benzyl aminopurine solution of 1500mg/L, and the grafting of new sprouting is carried out foliage-spray, whenever sprayed once at a distance from seven days, sprayed continuously 5 times;
The 3rd step, summer secondary grafting childrenization: about June 12, gathering first graft material is that the grafting second time is carried out in scion, and grafting method employing bud grafting is carried out, and grafting height 4～6cm, stock adopt 9501 clone pile seedlings;
The 4th step, growth hormone are handled: when treating that scion grows to the 15cm left and right sides, uses blotting paper to dip in to get concentration to be wrapped in the scion base portion of the new sprouting of grafting mouth, root induction as the growth hormone ABT1 of 2000mg/L;
The 5th step, grave mound takes root: the branch base portion that scion is sprouted carries out grave mound to be handled, and when scion is sprouted to 5～8cm, begins grave mound, and along with the growth of scion, grave mound carries out gradually, carries out grave mound three times to 6～8cm more than the grafting mouth, and keeps ground moistening;
The 6th goes on foot, buries root propagation: in late March, second is gathered paulownia cuttage seeding root segment to early April, and selecting root directly is about 1cm; Length is the root segment about 15cm, buries root propagation, strengthens water and fertilizer management; Prevention and elimination of disease and pests in time, and 7～mid-August in the ridging of nursery stock base portion once.
Embodiment 2:(material is the royal paulownia select tree)
A kind of method of paulownia select tree grafting grave mound childrenization, this method may further comprise the steps:
The first step, grafting rejuvenation: late March, the royal paulownia select tree branch that will collect early spring carries out grafting, and grafting method adopts bud grafting to carry out, and grafting height is 7cm overhead, and stock adopts 9501 clonal tissue culture seedlings;
Second step, the basic element of cell division are handled childrenization: since April 10, compound concentration was the basic element of cell division 6-benzyl aminopurine solution of 1800mg/L, and the grafting of new sprouting is carried out foliage-spray, and spraying method is whenever to spray once at a distance from seven days, sprays continuously 3 times;
The 3rd step, summer secondary grafting childrenization: about June 7, gathering first graft material is that the grafting second time is carried out in scion, and grafting method employing bud grafting is carried out, and grafting height 4～6cm, stock adopt 9501 clone pile seedlings;
The 4th step, growth hormone are handled, and when treating that scion grows to the 10cm left and right sides, use blotting paper to dip in to get concentration to be wrapped in the scion base portion of the new sprouting of grafting mouth, root induction as the growth hormone methyl of 1500mg/L;
The 5th step, grave mound takes root: scion begins grave mound when sprouting to 6cm, and along with the growth of scion, grave mound carries out gradually, carries out twice of grave mound to 6～8cm more than the grafting mouth, and keeps ground moistening;
The 6th goes on foot, buries root propagation: in late March, second is gathered paulownia cuttage seeding root segment to early April, and selecting root directly is about 1cm; Length is the root segment about 15cm, buries root propagation, strengthens water and fertilizer management; And prevention and elimination of disease and pests in time, and 7～mid-August in the ridging of nursery stock base portion once.
Embodiment 3:(material is a river paulownia select tree)
A kind of method of paulownia select tree grafting grave mound childrenization, this method may further comprise the steps:
The first step: the grafting rejuvenation, mid-September the river paulownia select tree branch of newly collecting is carried out grafting, grafting method adopts bud grafting to carry out, the overhead about 6cm of grafting height, stock adopts 9501 clonal tissue culture seedlings;
Second step, the basic element of cell division are handled childrenization: since April 21, compound concentration was the basic element of cell division 6-benzyl aminopurine solution of 2000mg/L, and the grafting of new sprouting is carried out foliage-spray, whenever at a distance from seven days once, sprayed continuously 3 times.
The 3rd step, summer secondary grafting childrenization: about June 15, gathering first graft material is that the grafting second time is carried out in scion, and grafting method employing bud grafting is carried out, and grafting height 4～6cm, stock adopt 9501 clone pile seedlings;
The 4th step, growth hormone are handled: when treating that scion grows to the 15cm left and right sides, use blotting paper to dip in and get the growth hormone naphthalene acid solution of concentration as 1000mg/L, be wrapped in the new scion base portion of sprouting of grafting mouth, root induction;
The 5th step, grave mound takes root, and scion begins grave mound when sprouting to 5～8cm, and along with the growth of scion, grave mound carries out gradually, carries out twice of grave mound to 6～8cm more than the grafting mouth, and keeps ground moistening;
The 6th goes on foot, buries root propagation: in late March, second is gathered paulownia cuttage seeding root segment to early April, and selecting root directly is about 1cm; Length is the root segment about 15cm, buries root propagation, strengthens water and fertilizer management; Prevention and elimination of disease and pests in time, and 7～mid-August in the ridging of nursery stock base portion once.
Claims (2)
1. the method for a paulownia select tree grafting grave mound childrenization is characterized in that this method may further comprise the steps:
The first step, grafting rejuvenation: the fortune paulownia of collecting in autumn, winter or early spring or royal paulownia or river paulownia or Formosan paulownia (Cortex seu Radix Paulowniae kawakamii) select tree branch are carried out grafting mid-September or late March; Grafting method adopts bud grafting to carry out; Grafting height is 6～8cm overhead, and stock adopts 9501 clonal tissue culture seedlings;
Second step, the basic element of cell division are handled the childrenization: early April, compound concentration was that the basic element of cell division 6-benzyladenine solution of 1500～2000mg/L carries out foliage-spray to grafting to late May, whenever at a distance from spray in 7～10 days once, sprayed continuously 3～5 times;
The 3rd the step, summer the secondary grafting childrenization: mid or late June, gathering first graft material is that the grafting second time is carried out in scion, and grafting height 4～6cm, stock adopt 9501 clone pile seedlings;
The 4th step, growth hormone are handled: when treating that scion is sprouted to 10～25cm, uses blotting paper to dip in to get concentration as the ABT1 growth hormone of 2000mg/L or use concentration to handle as the scion base portion that the methyl growth hormone of 1500mg/L is pasted on the sprouting of grafting mouth;
The 5th step, grave mound are taken root: the branch base portion that scion is sprouted carries out the grave mound processing, and the grave mound height is the above 6～8cm of grafting mouth, keeps ground moistening during the grave mound;
The 6th goes on foot, buries root propagation: in late March, second is gathered paulownia cuttage seeding root segment to early April, and selecting root directly is 0.5～1.5cm; The root segment of length 10～20cm buries root propagation, strengthens water and fertilizer management; Prevention and elimination of disease and pests in time, and July to mid-August in the ridging of nursery stock base portion once.
2. according to the method for claim 1 paulownia select tree grafting grave mound childrenization; It is characterized in that: it is to adopt repeatedly grave mound method that the 5th step grave mound is taken root, and when scion is sprouted to 5～8cm, begins grave mound, along with the growth of scion; Grave mound 2～3 times is to 6～8cm more than the grafting mouth, and keeps ground moistening.
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| CN104542004A (en) * | 2014-12-05 | 2015-04-29 | 安徽箐箐生态食品开发有限公司 | Grafting method of multiple varieties of kiwi fruit |
| CN104904500B (en) * | 2015-06-01 | 2017-07-14 | 广西壮族自治区林业科学研究院 | A kind of engrafting method using masson pine tissue-cultured seedling as stock |
| CN105917979A (en) * | 2016-06-16 | 2016-09-07 | 成都森洁商贸有限公司 | Operation method for grafting peonies |
| CN106941936A (en) * | 2017-04-20 | 2017-07-14 | 四川省林业科学研究院 | The big tree childrenization method of hardwood nanmu |
| CN107493988A (en) * | 2017-08-30 | 2017-12-22 | 辽宁省固沙造林研究所 | A kind of engrafting method of pinus sylvestris var. mongolica choiceness |
| CN107494125A (en) * | 2017-08-30 | 2017-12-22 | 长葛市联发粮食专业合作社 | A kind of method that paulownia bury root nursery |
| CN107306729A (en) * | 2017-09-20 | 2017-11-03 | 上海市园林科学规划研究院 | Asimina triloba buries root propagation method |
| CN110447403B (en) * | 2019-08-13 | 2022-04-22 | 中国林业科学研究院亚热带林业研究所 | Magnolia officinalis grafting propagation method |
| CN114190179B (en) * | 2021-12-22 | 2023-02-24 | 中国热带农业科学院橡胶研究所 | Method for improving bud grafting survival rate and strong seedling rate of rubber tree seedling bud grafting seedlings |
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