CN102042979A - 一种淀粉发酵液中葡萄糖含量的快速检测方法 - Google Patents
一种淀粉发酵液中葡萄糖含量的快速检测方法 Download PDFInfo
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- CN102042979A CN102042979A CN 201010522048 CN201010522048A CN102042979A CN 102042979 A CN102042979 A CN 102042979A CN 201010522048 CN201010522048 CN 201010522048 CN 201010522048 A CN201010522048 A CN 201010522048A CN 102042979 A CN102042979 A CN 102042979A
- Authority
- CN
- China
- Prior art keywords
- glucose
- potassium
- content
- amylofermentation
- invertase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 53
- 239000008103 glucose Substances 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 17
- 229920002472 Starch Polymers 0.000 title claims abstract description 13
- 239000008107 starch Substances 0.000 title claims abstract description 13
- 235000019698 starch Nutrition 0.000 title claims abstract description 13
- 238000000855 fermentation Methods 0.000 title abstract description 5
- 230000004151 fermentation Effects 0.000 title abstract description 5
- 238000001514 detection method Methods 0.000 claims abstract description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 12
- 230000003287 optical effect Effects 0.000 claims abstract description 9
- 238000006243 chemical reaction Methods 0.000 claims abstract description 7
- 238000010521 absorption reaction Methods 0.000 claims abstract description 4
- 239000007795 chemical reaction product Substances 0.000 claims abstract description 4
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims description 36
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 18
- 230000002829 reductive effect Effects 0.000 claims description 18
- -1 halide salts Chemical class 0.000 claims description 17
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims description 15
- 239000000975 dye Substances 0.000 claims description 15
- 239000001573 invertase Substances 0.000 claims description 15
- 235000011073 invertase Nutrition 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 15
- IOLCXVTUBQKXJR-UHFFFAOYSA-M potassium bromide Chemical compound [K+].[Br-] IOLCXVTUBQKXJR-UHFFFAOYSA-M 0.000 claims description 12
- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 claims description 12
- 108010015776 Glucose oxidase Proteins 0.000 claims description 9
- 239000004366 Glucose oxidase Substances 0.000 claims description 9
- 102000005548 Hexokinase Human genes 0.000 claims description 9
- 108700040460 Hexokinases Proteins 0.000 claims description 9
- 102000003992 Peroxidases Human genes 0.000 claims description 9
- 229940116332 glucose oxidase Drugs 0.000 claims description 9
- 235000019420 glucose oxidase Nutrition 0.000 claims description 9
- 229910052739 hydrogen Inorganic materials 0.000 claims description 9
- 239000001257 hydrogen Substances 0.000 claims description 9
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims description 9
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 9
- 239000001103 potassium chloride Substances 0.000 claims description 9
- 235000011164 potassium chloride Nutrition 0.000 claims description 9
- HHDOORYZQSEMGM-UHFFFAOYSA-L potassium;oxalate;titanium(4+) Chemical compound [K+].[Ti+4].[O-]C(=O)C([O-])=O HHDOORYZQSEMGM-UHFFFAOYSA-L 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- VVAKEQGKZNKUSU-UHFFFAOYSA-N 2,3-dimethylaniline Chemical compound CC1=CC=CC(N)=C1C VVAKEQGKZNKUSU-UHFFFAOYSA-N 0.000 claims description 6
- 102100031126 6-phosphogluconolactonase Human genes 0.000 claims description 6
- 108010029731 6-phosphogluconolactonase Proteins 0.000 claims description 6
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims description 6
- 102100022624 Glucoamylase Human genes 0.000 claims description 6
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 claims description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 6
- HIISVQYDQWJITQ-UHFFFAOYSA-N 1h-pyrrole;quinoline Chemical compound C=1C=CNC=1.N1=CC=CC2=CC=CC=C21 HIISVQYDQWJITQ-UHFFFAOYSA-N 0.000 claims description 5
- 230000005477 standard model Effects 0.000 claims description 5
- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 claims description 3
- 102000006995 beta-Glucosidase Human genes 0.000 claims description 3
- 108010047754 beta-Glucosidase Proteins 0.000 claims description 3
- 235000009518 sodium iodide Nutrition 0.000 claims description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 2
- 238000002835 absorbance Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 238000004448 titration Methods 0.000 description 10
- KVZZWQGMZOUUBC-UHFFFAOYSA-N 1H-pyrrol-2-amine quinoline Chemical compound NC=1NC=CC1.N1=CC=CC2=CC=CC=C12 KVZZWQGMZOUUBC-UHFFFAOYSA-N 0.000 description 4
- 239000012029 Fehling's reagent Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- BERDEBHAJNAUOM-UHFFFAOYSA-N copper(I) oxide Inorganic materials [Cu]O[Cu] BERDEBHAJNAUOM-UHFFFAOYSA-N 0.000 description 2
- AEJIMXVJZFYIHN-UHFFFAOYSA-N copper;dihydrate Chemical compound O.O.[Cu] AEJIMXVJZFYIHN-UHFFFAOYSA-N 0.000 description 2
- KRFJLUBVMFXRPN-UHFFFAOYSA-N cuprous oxide Chemical compound [O-2].[Cu+].[Cu+] KRFJLUBVMFXRPN-UHFFFAOYSA-N 0.000 description 2
- 229940112669 cuprous oxide Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- VZOPRCCTKLAGPN-ZFJVMAEJSA-L potassium;sodium;(2r,3r)-2,3-dihydroxybutanedioate;tetrahydrate Chemical compound O.O.O.O.[Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O VZOPRCCTKLAGPN-ZFJVMAEJSA-L 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 229940074446 sodium potassium tartrate tetrahydrate Drugs 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- QOGVBXAVOUAJIZ-UHFFFAOYSA-J [Cu+2].O.O.O.O.C(=O)([O-])C(O)C(O)C(=O)[O-].[K+].[Na+].C(=O)([O-])C(O)C(O)C(=O)[O-] Chemical compound [Cu+2].O.O.O.O.C(=O)([O-])C(O)C(O)C(=O)[O-].[K+].[Na+].C(=O)([O-])C(O)C(O)C(=O)[O-] QOGVBXAVOUAJIZ-UHFFFAOYSA-J 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 235000012736 patent blue V Nutrition 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000001476 sodium potassium tartrate Substances 0.000 description 1
- 235000011006 sodium potassium tartrate Nutrition 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102621140A (zh) * | 2012-02-17 | 2012-08-01 | 成都欧林生物科技股份有限公司 | 检测b型流感嗜血杆菌发酵液不同发酵时间剩余的葡萄糖浓度的方法 |
CN103278367A (zh) * | 2013-06-19 | 2013-09-04 | 广东省昆虫研究所 | 一种柑橘黄龙病快速诊断方法 |
CN103760161A (zh) * | 2014-01-25 | 2014-04-30 | 福州大学 | 一种葡萄糖的比色检测方法 |
CN104155253A (zh) * | 2014-09-09 | 2014-11-19 | 中国石油化工股份有限公司 | 一种快速检测生物表面活性剂产量的方法 |
CN107561070A (zh) * | 2017-08-28 | 2018-01-09 | 菏泽学院 | 一种葡萄糖快速可视化检测方法及其制备和应用 |
CN108458943A (zh) * | 2017-09-11 | 2018-08-28 | 中国石油天然气股份有限公司 | 催化裂化油浆糠醛精制油中糠醛含量的测定方法 |
CN109540802A (zh) * | 2018-12-13 | 2019-03-29 | 东北大学 | 一种生物体液中葡萄糖浓度的光声检测装置及方法 |
CN109580516A (zh) * | 2019-01-14 | 2019-04-05 | 广州城市职业学院 | 一种聚丙烯酰胺的检测方法 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108717086B (zh) * | 2018-05-03 | 2021-03-30 | 山西大学 | 一种测定食物中葡萄糖酸含量的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1133070A (zh) * | 1993-09-17 | 1996-10-09 | 微量科学有限公司 | 试剂 |
CN1982875A (zh) * | 2005-10-25 | 2007-06-20 | 霍夫曼-拉罗奇有限公司 | 吸收介质的荧光光谱学 |
-
2010
- 2010-10-28 CN CN 201010522048 patent/CN102042979B/zh not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1133070A (zh) * | 1993-09-17 | 1996-10-09 | 微量科学有限公司 | 试剂 |
CN1982875A (zh) * | 2005-10-25 | 2007-06-20 | 霍夫曼-拉罗奇有限公司 | 吸收介质的荧光光谱学 |
Non-Patent Citations (1)
Title |
---|
《郑州工程学院学报》 20030630 江秀明等 葡萄糖氧化酶-过氧化物酶偶联体系葡萄糖分析 第85-87页 1-5 第24卷, 第2期 * |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102621140A (zh) * | 2012-02-17 | 2012-08-01 | 成都欧林生物科技股份有限公司 | 检测b型流感嗜血杆菌发酵液不同发酵时间剩余的葡萄糖浓度的方法 |
CN103278367A (zh) * | 2013-06-19 | 2013-09-04 | 广东省昆虫研究所 | 一种柑橘黄龙病快速诊断方法 |
CN103278367B (zh) * | 2013-06-19 | 2015-06-24 | 广东省昆虫研究所 | 一种柑橘黄龙病快速诊断方法 |
US9921213B2 (en) | 2013-06-19 | 2018-03-20 | Guangdong Institute Of Applied Biological Resource | Rapid diagnosis method of citrus huanglongbing |
CN103760161A (zh) * | 2014-01-25 | 2014-04-30 | 福州大学 | 一种葡萄糖的比色检测方法 |
CN104155253A (zh) * | 2014-09-09 | 2014-11-19 | 中国石油化工股份有限公司 | 一种快速检测生物表面活性剂产量的方法 |
CN104155253B (zh) * | 2014-09-09 | 2016-09-14 | 中国石油化工股份有限公司 | 一种快速检测生物表面活性剂产量的方法 |
CN107561070A (zh) * | 2017-08-28 | 2018-01-09 | 菏泽学院 | 一种葡萄糖快速可视化检测方法及其制备和应用 |
CN108458943A (zh) * | 2017-09-11 | 2018-08-28 | 中国石油天然气股份有限公司 | 催化裂化油浆糠醛精制油中糠醛含量的测定方法 |
CN109540802A (zh) * | 2018-12-13 | 2019-03-29 | 东北大学 | 一种生物体液中葡萄糖浓度的光声检测装置及方法 |
CN109540802B (zh) * | 2018-12-13 | 2021-06-04 | 东北大学 | 一种生物体液中葡萄糖浓度的光声检测装置及方法 |
CN109580516A (zh) * | 2019-01-14 | 2019-04-05 | 广州城市职业学院 | 一种聚丙烯酰胺的检测方法 |
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