CN101970660A - 用于使脱靶效应最小化并使RNAi机制的饱和消除的新的siRNA结构及其用途 - Google Patents
用于使脱靶效应最小化并使RNAi机制的饱和消除的新的siRNA结构及其用途 Download PDFInfo
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- CN101970660A CN101970660A CN2008801269817A CN200880126981A CN101970660A CN 101970660 A CN101970660 A CN 101970660A CN 2008801269817 A CN2008801269817 A CN 2008801269817A CN 200880126981 A CN200880126981 A CN 200880126981A CN 101970660 A CN101970660 A CN 101970660A
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/14—Type of nucleic acid interfering N.A.
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- C12N2320/00—Applications; Uses
- C12N2320/50—Methods for regulating/modulating their activity
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Abstract
Description
Claims (6)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020070133416A KR100949791B1 (ko) | 2007-12-18 | 2007-12-18 | 오프-타겟 효과를 최소화하고 RNAi 기구를 포화시키지않는 신규한 siRNA 구조 및 그 용도 |
KR10-2007-0133416 | 2007-12-18 | ||
PCT/KR2008/007530 WO2009078685A2 (en) | 2007-12-18 | 2008-12-18 | Novel sirna structure for minimizing off-target effects and relaxing saturation of rnai machinery and the use thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101970660A true CN101970660A (zh) | 2011-02-09 |
CN101970660B CN101970660B (zh) | 2014-03-26 |
Family
ID=40796040
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200880126981.7A Active CN101970660B (zh) | 2007-12-18 | 2008-12-18 | 用于使脱靶效应最小化并使RNAi机制的饱和消除的新的siRNA结构及其用途 |
Country Status (7)
Country | Link |
---|---|
US (2) | US20120238017A1 (zh) |
EP (1) | EP2222848B1 (zh) |
JP (1) | JP5624474B2 (zh) |
KR (1) | KR100949791B1 (zh) |
CN (1) | CN101970660B (zh) |
AU (1) | AU2008339215B2 (zh) |
WO (1) | WO2009078685A2 (zh) |
Cited By (4)
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WO2017008755A1 (zh) * | 2015-07-15 | 2017-01-19 | 中国科学院上海生命科学研究院 | 新型shRNA表达载体及其制备和应用 |
CN108779463A (zh) * | 2016-02-02 | 2018-11-09 | 奥利克斯医药有限公司 | 使用靶向IL4Rα、TRPA1或F2RL1的RNA复合物治疗特应性皮炎和哮喘 |
CN108779464A (zh) * | 2016-02-02 | 2018-11-09 | 奥利克斯医药有限公司 | 使用靶向angpt2和pdgfb的rna复合物治疗血管生成相关性疾病 |
CN110982876A (zh) * | 2020-03-04 | 2020-04-10 | 圣湘生物科技股份有限公司 | 病毒核酸检测的预处理方法、预处理液、试剂盒及其用途 |
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US20100286378A1 (en) * | 2007-08-27 | 2010-11-11 | Boston Biomedical, Inc. | Composition of Asymmetric RNA Duplex As MicroRNA Mimetic or Inhibitor |
DK2548962T3 (en) | 2007-09-19 | 2016-04-11 | Applied Biosystems Llc | Sirna sequence-independent modification formats to reduce off-target phenotype effects in RNAI and stabilized forms thereof |
EP3336188B1 (en) * | 2008-09-22 | 2020-05-06 | Phio Pharmaceuticals Corp. | Reduced size self-delivering rnai compounds |
US8283460B2 (en) | 2008-10-15 | 2012-10-09 | Somagenics, Inc. | Short hairpin RNAs for inhibition of gene expression |
JP5592897B2 (ja) | 2008-12-26 | 2014-09-17 | サムヤン バイオファーマシューティカルズ コーポレイション | アニオン性薬物含有薬剤学的組成物及びその製造方法 |
US9493774B2 (en) | 2009-01-05 | 2016-11-15 | Rxi Pharmaceuticals Corporation | Inhibition of PCSK9 through RNAi |
US9745574B2 (en) | 2009-02-04 | 2017-08-29 | Rxi Pharmaceuticals Corporation | RNA duplexes with single stranded phosphorothioate nucleotide regions for additional functionality |
EP2756845B1 (en) * | 2009-04-03 | 2017-03-15 | Dicerna Pharmaceuticals, Inc. | Methods and compositions for the specific inhibition of KRAS by asymmetric double-stranded RNA |
US8871730B2 (en) | 2009-07-13 | 2014-10-28 | Somagenics Inc. | Chemical modification of short small hairpin RNAs for inhibition of gene expression |
US20150025122A1 (en) | 2009-10-12 | 2015-01-22 | Larry J. Smith | Methods and Compositions for Modulating Gene Expression Using Oligonucleotide Based Drugs Administered in vivo or in vitro |
US20130023578A1 (en) | 2009-12-31 | 2013-01-24 | Samyang Biopharmaceuticals Corporation | siRNA for inhibition of c-Met expression and anticancer composition containing the same |
US9080171B2 (en) | 2010-03-24 | 2015-07-14 | RXi Parmaceuticals Corporation | Reduced size self-delivering RNAi compounds |
KR20180044433A (ko) | 2010-03-24 | 2018-05-02 | 알엑스아이 파마슈티칼스 코포레이션 | 진피 및 섬유증성 적응증에서의 rna 간섭 |
EP2550001B1 (en) | 2010-03-24 | 2019-05-22 | Phio Pharmaceuticals Corp. | Rna interference in ocular indications |
ES2732929T3 (es) | 2010-10-22 | 2019-11-26 | Olix Pharmaceuticals Inc | Moléculas de ácido nucleico que inducen interferencia de ARN y usos de las mismas |
KR101590586B1 (ko) * | 2011-05-30 | 2016-02-01 | 성균관대학교산학협력단 | 표적 유전자 발현 억제 및 면역 반응을 동시에 유발하는 이중가닥의 긴 간섭 rna |
ES2716818T3 (es) | 2012-05-22 | 2019-06-17 | Olix Pharmaceuticals Inc | Molécula de ácido nucleico inductora de interferencias de arn capaz de penetrar en las células y uso de la misma |
RU2744194C2 (ru) | 2013-12-02 | 2021-03-03 | Фио Фармасьютикалс Корп | Иммунотерапия рака |
US11279934B2 (en) | 2014-04-28 | 2022-03-22 | Phio Pharmaceuticals Corp. | Methods for treating cancer using nucleic acids targeting MDM2 or MYCN |
KR101670254B1 (ko) * | 2014-07-09 | 2016-10-28 | 서울대학교산학협력단 | 목적 유전자의 발현 억제 특이성이 증가된 siRNA 분자 |
JP6836987B2 (ja) | 2014-09-05 | 2021-03-03 | フィオ ファーマシューティカルズ コーポレーションPhio Pharmaceuticals Corp. | Tyrまたはmmp1を標的とする核酸を用いて老化および皮膚障害を処置するための方法 |
WO2017007825A1 (en) | 2015-07-06 | 2017-01-12 | Rxi Pharmaceuticals Corporation | Methods for treating neurological disorders using a synergistic small molecule and nucleic acids therapeutic approach |
US11001845B2 (en) | 2015-07-06 | 2021-05-11 | Phio Pharmaceuticals Corp. | Nucleic acid molecules targeting superoxide dismutase 1 (SOD1) |
MX2018003096A (es) | 2015-09-15 | 2018-05-11 | Samyang Biopharmaceuticals | Composicion farmaceutica que contiene farmaco anionico, y metodo de preparacion para la misma. |
EP3365446A4 (en) | 2015-10-19 | 2019-06-26 | Phio Pharmaceuticals Corp. | SELF ADMINISTRATION-REDUCED SIZE NUCLEIC ACID COMPOUNDS TARGETING LONGS NON-CODING LONGS |
KR20180071362A (ko) | 2015-11-16 | 2018-06-27 | 올릭스 주식회사 | MyD88 또는 TLR3을 타겟팅하는 RNA 복합체를 이용한 연령-관련 황반 변성의 치료 |
EP3391875B1 (en) | 2015-12-18 | 2021-11-03 | Samyang Holdings Corporation | Method for preparing polymeric micelle containing anionic drug |
US10301628B2 (en) | 2016-04-11 | 2019-05-28 | Olix Pharmaceuticals, Inc. | Treatment of idiopathic pulmonary fibrosis using RNA complexes that target connective tissue growth factor |
KR101916652B1 (ko) | 2016-06-29 | 2018-11-08 | 올릭스 주식회사 | 작은 간섭 rna의 rna 간섭효과 증진용 화합물 및 이의 용도 |
CN110770343A (zh) | 2017-02-10 | 2020-02-07 | 成均馆大学校产学协力团 | 用于rna干扰的长双链rna |
KR102321426B1 (ko) | 2017-02-21 | 2021-11-05 | 올릭스 주식회사 | 남성형 탈모 표적 유전자의 발현을 억제하는 비대칭 siRNA |
KR20190037166A (ko) * | 2017-09-28 | 2019-04-05 | 올릭스 주식회사 | 결합 조직 성장 인자를 표적으로 하는 rna 복합체를 함유하는 노인성 황반변성의 예방 또는 치료용 약학 조성물 |
EP3974531A4 (en) * | 2019-05-20 | 2024-01-10 | Olix Pharmaceuticals Inc | ASYMMETRIC SIRNA INHIBITING EXPRESSION OF PD-1 |
EP4159858A1 (en) * | 2020-05-26 | 2023-04-05 | Olix Pharmaceuticals, Inc. | Rnai agent targeting myd88 and use thereof |
Family Cites Families (22)
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JP2003515323A (ja) * | 1999-11-18 | 2003-05-07 | オックスフォード バイオメディカ(ユーケイ)リミテッド | 抗 体 |
DE10160151A1 (de) * | 2001-01-09 | 2003-06-26 | Ribopharma Ag | Verfahren zur Hemmung der Expression eines vorgegebenen Zielgens |
EP1873259B1 (en) * | 2000-12-01 | 2012-01-25 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | RNA interference mediated by 21 and 22nt RNAs |
US20080188430A1 (en) * | 2001-05-18 | 2008-08-07 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of hypoxia inducible factor 1 (HIF1) gene expression using short interfering nucleic acid (siNA) |
US7043569B1 (en) * | 2001-09-07 | 2006-05-09 | Chou Norman C | Method and system for configuring an interconnect device |
US20040138163A1 (en) * | 2002-05-29 | 2004-07-15 | Mcswiggen James | RNA interference mediated inhibition of vascular edothelial growth factor and vascular edothelial growth factor receptor gene expression using short interfering nucleic acid (siNA) |
EP1527176B2 (en) * | 2002-08-05 | 2017-03-22 | Silence Therapeutics GmbH | Further novel forms of interfering rna molecules |
WO2004032524A1 (ja) * | 2002-09-12 | 2004-04-15 | Matsushita Electric Industrial Co., Ltd. | 画像処理装置 |
PT2284266E (pt) * | 2002-11-14 | 2013-12-17 | Thermo Fisher Scient Biosciences Inc | Siarn contra tp53 |
EP3450559A1 (en) * | 2003-03-07 | 2019-03-06 | Alnylam Pharmaceuticals, Inc. | Therapeutic compositions |
US20050136437A1 (en) * | 2003-08-25 | 2005-06-23 | Nastech Pharmaceutical Company Inc. | Nanoparticles for delivery of nucleic acids and stable double-stranded RNA |
WO2005062937A2 (en) * | 2003-12-22 | 2005-07-14 | University Of Massachusetts | Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended sirna |
US20060134787A1 (en) * | 2004-12-22 | 2006-06-22 | University Of Massachusetts | Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended siRNA |
US20060069050A1 (en) * | 2004-02-17 | 2006-03-30 | University Of Massachusetts | Methods and compositions for mediating gene silencing |
KR101147147B1 (ko) * | 2004-04-01 | 2012-05-25 | 머크 샤프 앤드 돔 코포레이션 | Rna 간섭의 오프 타겟 효과 감소를 위한 변형된폴리뉴클레오타이드 |
US20060142228A1 (en) * | 2004-12-23 | 2006-06-29 | Ambion, Inc. | Methods and compositions concerning siRNA's as mediators of RNA interference |
US20070218495A1 (en) * | 2006-03-16 | 2007-09-20 | Dharmacon, Inc. | Methods, libraries and computer program products for gene silencing with reduced off-target effects |
PT2164967E (pt) * | 2007-05-31 | 2015-10-27 | Univ Iowa Res Found | Redução da toxicidade não dirigida do arn de interferência |
US20090020856A1 (en) * | 2007-07-17 | 2009-01-22 | International Business Machines Corporation | Semiconductor device structures and methods for shielding a bond pad from electrical noise |
US20100286378A1 (en) * | 2007-08-27 | 2010-11-11 | Boston Biomedical, Inc. | Composition of Asymmetric RNA Duplex As MicroRNA Mimetic or Inhibitor |
EP3336188B1 (en) * | 2008-09-22 | 2020-05-06 | Phio Pharmaceuticals Corp. | Reduced size self-delivering rnai compounds |
CN108138181B (zh) * | 2015-07-27 | 2022-05-24 | 奥利克斯医药有限公司 | 抑制黑色素生成的rna复合物 |
-
2007
- 2007-12-18 KR KR1020070133416A patent/KR100949791B1/ko active IP Right Grant
-
2008
- 2008-12-18 JP JP2010539301A patent/JP5624474B2/ja active Active
- 2008-12-18 CN CN200880126981.7A patent/CN101970660B/zh active Active
- 2008-12-18 WO PCT/KR2008/007530 patent/WO2009078685A2/en active Application Filing
- 2008-12-18 US US12/808,772 patent/US20120238017A1/en not_active Abandoned
- 2008-12-18 AU AU2008339215A patent/AU2008339215B2/en active Active
- 2008-12-18 EP EP08861334.4A patent/EP2222848B1/en active Active
-
2017
- 2017-08-16 US US15/678,823 patent/US20180127747A1/en active Pending
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017008755A1 (zh) * | 2015-07-15 | 2017-01-19 | 中国科学院上海生命科学研究院 | 新型shRNA表达载体及其制备和应用 |
CN106636084A (zh) * | 2015-07-15 | 2017-05-10 | 中国科学院上海生命科学研究院 | 新型shRNA表达载体及其制备和应用 |
CN108779463A (zh) * | 2016-02-02 | 2018-11-09 | 奥利克斯医药有限公司 | 使用靶向IL4Rα、TRPA1或F2RL1的RNA复合物治疗特应性皮炎和哮喘 |
CN108779464A (zh) * | 2016-02-02 | 2018-11-09 | 奥利克斯医药有限公司 | 使用靶向angpt2和pdgfb的rna复合物治疗血管生成相关性疾病 |
CN108779463B (zh) * | 2016-02-02 | 2022-05-24 | 奥利克斯医药有限公司 | 使用靶向IL4Rα、TRPA1或F2RL1的RNA复合物治疗特应性皮炎和哮喘 |
CN110982876A (zh) * | 2020-03-04 | 2020-04-10 | 圣湘生物科技股份有限公司 | 病毒核酸检测的预处理方法、预处理液、试剂盒及其用途 |
WO2021174673A1 (zh) * | 2020-03-04 | 2021-09-10 | 圣湘生物科技股份有限公司 | 病毒核酸检测的预处理方法、预处理液、试剂盒及其用途 |
Also Published As
Publication number | Publication date |
---|---|
AU2008339215A8 (en) | 2011-11-03 |
WO2009078685A2 (en) | 2009-06-25 |
EP2222848A2 (en) | 2010-09-01 |
EP2222848A4 (en) | 2011-06-22 |
EP2222848B1 (en) | 2015-02-25 |
JP5624474B2 (ja) | 2014-11-12 |
US20120238017A1 (en) | 2012-09-20 |
WO2009078685A3 (en) | 2009-09-17 |
AU2008339215A1 (en) | 2009-06-25 |
JP2011505868A (ja) | 2011-03-03 |
KR20090065880A (ko) | 2009-06-23 |
KR100949791B1 (ko) | 2010-03-30 |
AU2008339215B2 (en) | 2013-05-23 |
CN101970660B (zh) | 2014-03-26 |
US20180127747A1 (en) | 2018-05-10 |
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Application publication date: 20110209 Assignee: Olis Ltd. Assignor: Li Dongqi Contract record no.: 2017990000336 Denomination of invention: Novel sirna structure for minimizing off-target effects and relaxing saturation of rnai machinery and the use thereof Granted publication date: 20140326 License type: Common License Record date: 20170823 Application publication date: 20110209 Assignee: HUGEL Inc. Assignor: Olis Ltd. Contract record no.: 2017990000337 Denomination of invention: Novel sirna structure for minimizing off-target effects and relaxing saturation of rnai machinery and the use thereof Granted publication date: 20140326 License type: Common License Record date: 20170823 |
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Assignee: HUGEL Inc. Assignor: Olis Ltd. Contract record no.: 2017990000337 Date of cancellation: 20230704 |