CN101969759A - 具有增强的产量相关性状的植物及其制备方法 - Google Patents
具有增强的产量相关性状的植物及其制备方法 Download PDFInfo
- Publication number
- CN101969759A CN101969759A CN2008801202018A CN200880120201A CN101969759A CN 101969759 A CN101969759 A CN 101969759A CN 2008801202018 A CN2008801202018 A CN 2008801202018A CN 200880120201 A CN200880120201 A CN 200880120201A CN 101969759 A CN101969759 A CN 101969759A
- Authority
- CN
- China
- Prior art keywords
- plant
- sequence
- nucleic acid
- polypeptide
- domain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Abstract
本发明总体上涉及分子生物学领域并涉及用于通过调节植物中编码TCP1、或TCP2转录因子的核酸的表达而增强多种植物产量相关性状的方法。本发明也涉及具有编码TCP1、或TCP2多肽的核酸的受调节表达的植物,所述植物相对于对应野生型植物或其他对照植物具有增强的产量相关性状。本发明也提供了用于本发明方法中的构建体。在另一实施方案中,本发明涉及用于通过调节编码底物蛋白-样多肽的核酸或编码tRNAδ(2)-异戊烯焦磷酸转移酶(IPPT)多肽的核酸序列在植物中的表达而增强植物产量相关性状的方法,以及用于增强在亚最佳养分可利用性条件下培育的植物的产量相关性状的方法,其包括调节编码SHORT-ROOT(SHR)多肽的核酸在植物中的表达。
Description
本申请要求以下申请的优先权:2007年12月20日提交的EP07123820.8;2008年2月11日提交的US 61/027,513;2007年12月21日提交的EP 07124011.3;2008年2月8日提交的US 61/027,105;2007年12月21日提交的EP 07124036.0;2008年2月8日提交的US61/027,155;2007年12月24日提交的EP 07125090.7和2008年2月11日提交的US 61/027,499,这些申请的全部内容在此引入作为参考。
本发明总体上涉及分子生物学领域并涉及用于通过调节植物中编码TCP1、或TCP2转录因子的核酸的表达而增强多种植物产量相关性状的方法。本发明也涉及具有编码TCP1、或TCP2多肽的核酸的受调节表达的植物,所述植物相对于对应野生型植物或其他对照植物具有增强的产量相关性状。本发明也提供了用于本发明方法中的构建体。
在另一实施方案中,本发明涉及用于通过调节编码底物蛋白-样多肽的核酸在植物中的表达来改善多种植物生长特征的方法。本发明还涉及具有编码底物蛋白-样多肽的核酸的调节的表达的植物,所述植物相对于对应的野生型植物或其他对照植物具有改善的生长特征。本发明也提供了用于本发明方法中的构建体。
在另一实施方案中,本发明涉及用于通过增加编码tRNAδ(2)-异戊烯焦磷酸转移酶(IPPT)多肽的核酸序列在植物种子中的表达来增强各种植物产量相关性状的方法。本发明还涉及具有编码IPPT多肽的核酸在种子中增加的表达的植物,所述植物相对于对照植物具有增强的产量相关性状。此外,本发明涉及包含所述核酸序列的核酸构建体、载体和植物。
在另一实施方案中,本发明涉及用于增强在亚最佳养分可利用性条件下培育的植物的产量相关性状的方法,其包括调节编码SHORT-ROOT(SHR)多肽的核酸在植物中的表达。本发明还提供相对于对照植物提高植物千粒重(TKW)的方法,其包括调节编码SHR多肽的核酸在无养分限制条件下培育的植物中的表达。本发明还涉及具有编码SHR多肽的核酸的调节的表达的植物,所述植物相对于对应的野生型植物或其他对照植物具有增强的产量相关性状。本发明也提供了用于本发明方法中的构建体。
持续增长的世界人口和农业用可耕地供应萎缩刺激了有关提高农业效率的研究。常规的作物及园艺学改良手段利用选择育种技术以鉴定具有受欢迎特征的植物。然而,此类选择育种技术具有几个缺陷,即这些技术一般耗费很多劳动并且产生这样的植物,其经常含有异源的遗传组分,其可能不总导致受欢迎性状从亲代植物传递下去。分子生物学进展已经允许人类改良动物及植物的种质。植物的遗传工程使得可以分离和操作遗传物质(一般处于DNA或RNA形式)并且随后导入该遗传物质至植物中。此类技术具有产生具备多种经济学、农学或园艺学改良性状的作物或植物的能力。
具有特殊经济意义的性状是提高的产量。产量通常定义为来自作物的经济价值的可测量结果。该结果可以就数量和/或品质方面进行定义。产量直接取决于几个因素,例如器官的数目和大小、植物构造(例如枝的数目)、种子产生、叶衰老等。根发育、养分摄入、胁迫耐受性和早期生长势(早期生长势)也可以是决定产量的重要因素。优化前述因素因而可以有助于提高作物产量。
种子产量是特别重要的性状,因为许多植物的种子对人和动物营养是重要的。作物如谷物、稻、小麦、卡诺拉油菜和大豆占超过一半的人类总热量摄入,无论通过直接消费种子本身或通过消费基于加工的种子而产生的肉产品。作物也是糖、油及工业加工中所用许多类型代谢物的来源。种子含有胚(新枝条(shoot)和新根的起源)和胚乳(萌发期间和籽苗早期生长期间用于胚生长的养分来源)。种子发育涉及多种基因并且需要代谢物从根、叶和茎转移至正在生长的种子中。胚乳尤其同化糖类、油和蛋白质的代谢前体并且将它们合成为贮藏大分子以灌满籽粒。
植物生物量是饲料作物如苜蓿、饲用谷物和干草的产量。产量的许多代用物已经用于谷物作物。它们当中主要是对植物尺寸的估计。植物尺寸可以根据物种和发育阶段以多种方式测量,不过包括总植物干重、地上部分干重、地上部分鲜重、叶面积、茎体积、植物高度、莲座丛直径、叶长度、根长度、根质量、分蘖数和叶数。许多物种维持在给定发育阶段上植物不同部分的尺寸之间的保守比率。这些异速增长关系用来从这些尺寸量值之一外推至另一种尺寸量值(例如Tittonell等,2005Agric Ecosys & Environ 105:213)。在发育早期的植物尺寸一般与发育中稍后的植物尺寸相关。具有较大叶面积的较大植物通常可以比较小的植物吸收更多光线和二氧化碳并且因而可能会在相同的时段期间获得更大重量(Fasoula和Tollenaar 2005Maydica50:39)。此外,这也是植物应当初始实现较大尺寸的微环境优势或遗传优势的潜在延续。存在针对植物尺寸和生长速率的强遗传组分(例如terSteege等,2005Plant Physiology 139:1078),并且因而对于一系列各异遗传表型,在一种环境条件下的植物尺寸很可能关联于另一种环境条件下的尺寸(Hittalmani等,2003Theoretical AppliedGenetics 107:679)。以这种方式,使用标准环境作为作物在田间于不同位置及时间所遭遇的多样且动态环境的代用物。
对于许多作物的另一个重要性状是早期生长势。改善早期生长势是现代稻育种计划在温带和热带稻品种方面的重要目标。长根对于水栽稻中正确土壤固定是重要的。在稻直接播种至被淹没田地的情况下,以及在植物必须从水中迅速萌发的情况下,较长的枝条与生长势相关。在实施条播的情况下,较长的中胚轴和胚芽鞘对良好出苗是重要的。将早期生长势人工改造到植物内的能力在农业中将是极重要的。例如,不良的早期生长势已经限制了基于玉米带种质(Corn Belt germplasm)在欧洲大西洋地区引种玉蜀黍(Zea mayes L.)杂种。
收获指数即种子产量对地上部分干重的比率在许多环境条件下是相对稳定的并且因而可以经常获得植物尺寸与谷物产量之间的强相关(例如Rebetzke等,2002Crop Science 42:739)。这些过程是内在联系的,因为谷物生物量的主要部分取决于植物叶和茎的现有和储备光合生产率(Gardener等,1985Physiology of Crop Plants.Iowa StateUniversity Press,第68-73页)。因此,选择植物尺寸(甚至在发育的早期)已经用作未来潜在产能的指示物(例如Tittonell等,2005AgricEcosys & Environ 105:213)。当检验遗传差异对胁迫耐受性的影响时,使土壤属性、温度、水和养分有效性和光强度标准化的能力是温室或植物生长室环境与田间相比的固有优势。然而,产量因不良授粉所致的人为限制可能限制这些受控环境检验产量差异的用途,其中所述的不良授粉因缺少风和昆虫或成熟根或根冠生长的足够空间引起。因此,在标准化条件下在生长室或温室测量发育早期的植物尺寸是提供潜在遗传产量优势指示的标准操作。
另一个重要性状是改良的非生物性胁迫耐受性。非生物性胁迫是世界范围作物损失的主要原因,对于大多数主要作物植物而言平均产量降低超过50%(Wang等,Planta(2003)218:1-14)。非生物性胁迫可以由干旱、盐度、极端温度、化学毒性、养分过量或匮乏(大分子和/或微量元素)、辐射和氧化胁迫引起。提高植物的非生物性胁迫耐受性能力将在世界范围对农民是巨大的经济优势并且将允许在不利条件期间及在本来不可能栽培作物的陆地上栽培作物。
因而可以通过优化前述因素之一提高作物产量。
取决于最终用途,对某些产量性状的改良可能优先于其他产量性状。例如对于应用如饲料或木材生产或生物燃料资源而言,增加植物营养性部分可能是希望的,而对于应用如面粉、淀粉或油生产而言,种子参数提高可能是特别希望的。即便在种子参数当中,某些参数可以更优先于其他参数,这取决于用途。多种机制可以有助于提高种子产量,无论其形式为提高的种子尺寸或提高的种子数目。
增强植物中产量相关性状(种子产量和/或生物量)的一种方法可以是通过调节植物的内在生长机制,如细胞周期或参与植物生长或参与防御机制的多种信号发放途径。
现在已经发现可以通过调节植物中编码如本文定义的TCP1或TCP2,或底物蛋白-样,或SHR多肽的核酸的表达而增强植物中多种产量相关性状。
在另一实施方案中,现在已经发现可以通过增加植物种子中编码tRNAδ(2)-异戊烯焦磷酸转移酶(IPPT)多肽的核酸序列的表达而相对于对照植物增强植物中多种产量相关性状。增强的产量相关性状包括如下性状之一种或多种:提高的早期生长势、提高的地上生物量、提高的每株植物种子总产量、提高的种子总数、提高的饱满种子数、提高的每穗花数和提高的收获指数。
背景技术
TCP/TCP2多肽
转录因子通常定义为显示序列特异性DNA结合亲和力,并能够激活和/或抑制转录的蛋白质。拟南芥(Arabidopsis thaliana)基因组编码至少1533种转录调节物,占其估计的基因总数的~5.9%(Riechmann等(2000)Science 290:2105-2109)。转录因子的TCP家族是根据其首次被表征的成员命名的(teosinte-branched1(TB1)、cycloidea(CYC)和PCNA因子(PCF);Cubas P等(1999)Plant J 18(2):215-22)。在拟南芥中,已经鉴定了超过20个TCP家族多肽成员,并根据TCP结构域中的序列相似性分为正调节基因表达的Ⅰ类(也称为Ⅰ组或PCF组)转录因子和负调节增殖的Ⅱ类(也称为Ⅱ组或CYC-TB1组)转录因子。所有TCP转录因子的特征在于非经典的预测的碱性-螺旋-环-螺旋(bHLH),其对DNA结合及同源二聚化和异源二聚化都是必需的(见上文Cubas等)。
令人惊奇地,现已发现,提高编码TCP1或TCP2转录因子的核酸序列在植物中的表达使植物相对于对照植物具有增强的产量相关性状。下文详述了适合用于增强产量相关性状的TCP多肽的具体亚组。
根据一个实施方案,提供了相对于对照植物在植物中增强产量相关性状的方法,其包括调节编码TCP1或TCP2多肽的核酸在植物中的表达。
底物蛋白-样蛋白质
真核细胞具有在分子摄取(胞吞作用)或分子递送至细胞外部(分泌途径)中起作用的精细膜系统。分泌途径从内质网起始,经高尔基体至细胞膜。胞吞途径从细胞膜至细胞内部。所有这些途径都利用小泡,小泡从其所产生自的细胞器出芽,其内含物和其目的地具有高度选择性。新合成的蛋白质需转运至不同的亚细胞部分或外排至细胞外环境。细胞内运输由许多蛋白质控制,这些蛋白质例如是小泡的部分、或协助小泡形成或融合、或调节运输或协助货物蛋白质的选择等。这些蛋白质中许多是植物、酵母和动物共有的,这表明细胞内运输结构在真核生物中是保守的。此组蛋白质中的一种的特征在于存在保守的“底物蛋白N端同源”(ENTH)结构域。ENTH结构域能够结合至磷脂酰肌醇,因此被认为在将这些蛋白质靶向至特定区室和协助网格蛋白介导的出芽中发挥作用。ANTH(AP180N端同源)结构域被假定具有与ENTH结构域类似的功能,但其是部分结构上不同的蛋白质。
底物蛋白-样蛋白质包含ENTH结构域,并被假定在网格蛋白包被的小泡形成中发挥类似作用;据报道底物蛋白-样蛋白质与多种蛋白质相互作用(Lee等,Plant Physiology 143,1561-1575,2007;Song等,PlantCell 18,2258-2274,2006)。
腺苷酸-IPT(AMP异戊基转移酶/ATP/ADP异戊基转移酶)
植物激素响应内源和环境刺激而控制植物的生长和发育。植物激素的实例包含脱落酸、生长素、细胞分裂素、乙烯、赤霉素、油菜素内酯、水杨酸、茉莉酸类、信号发放肽和系统素。
在植物中,天然存在的细胞激动素(CK)构成一组具有异戊烯基侧链(类异戊二烯CK;最丰富的类型)或芳基(芳族CK;稀少)的腺嘌呤衍生物,其在植物发育中发挥重要作用。类异戊二烯CK生物合成的第一步和限速步骤由异戊烯基转移酶催化,其将异戊烯基部分从δ(2)-二甲基丙烯基二磷酸(DMAPP)或羟甲基丁烯基二磷酸(HMBDP)转移至缀合腺嘌呤的N6位上。根据其利用哪一种缀合腺嘌呤,可将异戊基转移酶进一步分为三个亚组:
1)AMP异戊基转移酶类(也称为DMAPP:AMP异戊基转移酶,EC 2.5.1.27),其优选使用腺苷5’-单磷酸作为受体分子;典型的实例见于致植物病细菌中,如根瘤农杆菌(Agrobacterium tumefaciens)、丁香假单胞杆菌(Pseudomonas syringae)、青枯假单胞杆菌(Pseudomonassolanacearum)(青枯雷尔氏菌(Ralstonia solanacearum))和成团肠杆菌(Pantoea agglomerans)(草生欧文氏菌(Erwinia herbicola))、固氮共生蓝藻念珠藻属(Nostoc)或黏菌Disctyostelium discoideum。
2)ATP/ADP异戊基转移酶类(也称为DMAPP:ATP/ADP异戊基转移酶),其优选使用腺苷5’-三磷酸或腺苷5’-二磷酸作为受体分子;例如在拟南芥中发现了8种ATP/ADP异戊基转移酶(Miyawaki等(2006)Proc Natl Acad Sci USA 103(44):16598-16603)。
3)tRNA异戊基转移酶类(也称为DMAPP:tRNA异戊基转移酶或rRNAδ(2)异戊烯焦磷酸转移酶(IPPT),EC 2.5.1.8),其优选使用某些tRNA(定位于细胞质内、质体内和线粒体内)紧靠反密码子的37位腺嘌呤;已纯化了该酶,并从细菌、酵母、动物和植物中克隆了其基因。
前两组(共同称为腺苷酸-IPT)催化游离细胞激动素的直接从头生物合成,其基本上由异戊烯腺嘌呤(iP)-型和反式玉米素(tZ)-型细胞激动素组成。第三组(称为tRNA-IPT或IPPT)通过tRNA的异戊烯基化催化细胞激动素形成,其降解时释放细胞激动素核苷酸,其反过来被用于生物合成顺式玉米素(cZ)-型细胞激动素。因此,tRNA周转率也强烈地决定游离细胞激动素核苷酸的可用性。
虽然在原核生物中tRNA是游离细胞激动素的一般来源(Koenig等(2002)J Bacteriol 184:1832-1842),但在种子植物中tRNA-IPT和腺苷酸-IPT途径都促成细胞激动素的生物合成(Miyawaki等(2006)Proc Natl Acad Sci USA 103(44):16598-16603)。然而,一般认为tRNA途径不足以成为种子植物中细胞激动素的主要来源。最后,这两条生物合成途径导致按不同比例合成不同的细胞激动素。
腺苷酸-IPT和tRNA-IPT在其N端都具有ATP/GTP P-环结合基序(A,G)-X4-G-K-(S,T)。对真核生物tRNA-IPT特异而在原核生物tRNA-IPT中缺乏的另一种公知的保守区域定位于C端:锌指-样基序C2H2(C-X2-C-X(12,18)-H-X5-H。锌指-样基序在tRNA-IPT中的功能可能与蛋白质-蛋白质相互作用和核定位相关(Golovko等(2000)Gene258:85-93)。
当来自根瘤农杆菌的腺苷酸-IPT在植物中组成性过量表达、或较弱地或条件性表达时,植物显示细胞激动素过量产生的典型效应,如不受控制的腋芽生长(降低的顶端优势)、小卷缩叶的形成、延迟的根系形成和改变的衰老(例如Luo等(2005)Plant Growth Regulation47:1-47和其中的参考文献)。
与对照植物相比,在种子特异启动子控制下表达细菌腺苷酸-IPT的转基因拟南芥和卡诺拉油菜植物每株植物的平均种子产量没有显著提高(Roeckel等(1997)Transgenic Res 6(2):133-41)。
美国专利申请2006/0010515描述了转基因拟南芥,其独立地用三个细胞周期调节启动子表达来自根瘤农杆菌的腺苷酸-IPT,该植物具有增加的叶尺寸/营养性、增加的植物高度、增加的枝条数、增加的花和长角果数。
SHORT ROOT(SHR)
GRAS基因家族(根据已知基因GAI、RGA和SCR的首字母缩拼词命名)的成员编码在植物生长和发育如赤霉素信号转导、根辐射形态、腋生分生组织形成、植物色素A信号转导和配子发生中具有多种功能的转录调节物。系统发生分析将GRAS基因家族分为具有不同保守结构域和功能的八个亚家族(Tian等,(2004)Plant MolecularBiology,第54卷,第4期,第519-532页)。GRAS蛋白质包含约350个氨基酸的保守区域,其可分为见于以下顺序的5个基序:亮氨酸七残基重复序列Ⅰ、VHIID基序、亮氨酸七残基重复序列Ⅱ、PFYRE基序和SAW基序。
SHORT ROOT或SHR是植物转录因子GRAS家族的成员,且是涉及根系发育的蛋白质。
已授权的专利US 6,927,320B1描述了SHR基因,公开了SHR基因表达控制根内某些细胞类型的细胞分裂、影响根和茎的组织和影响气生结构的向重力性。已提出SHR表达水平的调节可以用来改变转基因植物的根和气生结构和增强此类植物的农学特性。还提出用SHR过量表达改造的植物可以显示改良的旺盛生长特征,其可通过检测任意以下参数来鉴定:1.生长速率;2.成熟植物的营养性产量;3.种子或果实产量;4.种子或果实重量;5.植物的总氮含量;6.果实或种子的总氮含量;7.植物的游离氨基酸含量;8.果实或种子的游离氨基酸含量;9.植物的总蛋白质含量和10.果实或种子的总蛋白质含量。
发明概述
令人惊奇地,现已发现调节编码TCP或TCP2多肽的核酸的表达产生相对于对照植物具有增强的产量相关性状,尤其是提高的产量和种子产量的植物。
还令人惊奇地,现已发现调节编码底物蛋白-样多肽的核酸的表达产生相对于对照植物具有增强的产量相关性状,尤其是提高的产量和/或提高的早期生长势的植物。
一个实施方案提供了相对于对照植物增强植物产量相关性状的方法,其包括调节编码底物蛋白-样多肽的核酸在植物中的表达。该增强的产量相关性状包括提高的产量和/或提高的早期生长势。
此外,令人惊奇地,现已发现在植物种子中增加编码IPPT多肽的核酸序列的表达产生相对于对照植物具有增强的产量相关性状的植物。
一个实施方案提供了相对于对照植物在植物中增强产量相关性状的方法,其包括提高编码如本文定义的IPPT多肽的核酸序列在植物种子中的表达。该增强的产量相关性状包含以下一种或多种:提高的早期生长势、提高的地上部分生物量、提高的每株植物种子总产量、提高的种子总数、提高的饱满种子数、提高的每穗花数和提高的收获指数。
此外,令人惊奇地,现已发现在亚最佳养分可利用性条件下生长的植物中调节编码SHR多肽的核酸的表达产生相对于对照植物具有增强的产量相关性状的植物。还令人惊奇地,现已发现在无养分限制条件下生长的植物中调节编码SHR多肽的核酸的表达产生相对于对照植物具有提高的千粒重(TKW)的植物。
本发明的一个实施方案提供了相对于对照植物增强植物产量相关性状的方法,其包括调节编码SHR多肽的核酸在亚最佳养分可利用性条件下生长的植物中的表达。
本发明的另一实施方案提供了相对于对照植物提高植物千粒重(TKW)的方法,其包括调节编码SHR多肽的核酸在无养分限制条件下生长的植物中的表达。
定义
多肽/蛋白质
术语“多肽”和“蛋白质”在本文中可相互交换地使用并且指由肽键连接起来的任意长度聚合物形式的氨基酸。
多核苷酸/核酸/核酸序列/核苷酸序列
术语“多核苷酸”、“核酸序列”、“核苷酸序列”、“核酸”、“核酸分子”在本文中可相互交换地使用并且指任意长度的聚合非分支形式的核苷酸,即核糖核苷酸或脱氧核糖核苷酸或这二者的组合。
对照植物
选择合适的对照植物是实验设计的例行部分并且可以包括相应的野生型植物或无目的基因的相应植物。对照植物一般是相同的植物物种或甚至是与待评估植物相同的品种。对照植物也可以是待评估植物的失效合子。失效合子是因分离而丢失转基因的个体。如本文中所用的“对照植物”不仅指完整植物,也指植物部分,包括种子和种子部分。
同源物
蛋白质的“同源物”包括这样的肽、寡肽、多肽、蛋白质和酶,它们相对于非修饰的所讨论蛋白质具有氨基酸取代、缺失和/或插入并且与衍生它们的非修饰蛋白质具有相似的生物学活性和功能活性。
缺失指从蛋白质中移除一个或多个氨基酸。
插入指一个或多个氨基酸残基被导入蛋白质中的预定位点。插入可以包含氨基端融合和/或羧基端融合以及序列内插入单个或多个氨基酸。通常,在氨基酸序列内部的插入物比氨基端融合物或羧基端融合物小约1至10个残基级别。氨基端或羧基端融合蛋白或融合肽的实例包括如酵母双杂交系统中所用的转录激活物的结合结构域或激活结构域、噬菌体外壳蛋白、(组氨酸)-6-标签、谷胱甘肽S-转移酶-标签、蛋白A、麦芽糖结合蛋白、二氢叶酸还原酶、Tag·100表位、c-myc表位、-表位、lacZ、CMP(钙调蛋白结合肽)、HA表位、蛋白C表位和VSV表位。
取代指以具有相似特性(如相似的疏水性、亲水性、抗原性、形成或破坏α-螺旋结构或β-折叠结构的倾向性)的其他氨基酸替代蛋白质的氨基酸。氨基酸取代一般是单个残基的,但是根据给予多肽的功能性约束条件,可以是簇集的;插入通常是约1至10个氨基酸残基级别。氨基酸取代优选是保守性氨基酸取代。保守性取代表是本领域熟知的(见例如Creighton(1984)Proteins.W.H.Freeman和Company(编)和下表1)。
表1:保守性氨基酸取代的实例
残基 | 保守性取代 | 残基 | 保守性取代 |
Ala | Ser | Leu | Ile;Val |
Arg | Lys | Lys | Arg;Gln |
Asn | Gln;His | Met | Leu;Ile |
Asp | Glu | Phe | Met;Leu;Tyr |
Gln | Asn | Ser | Thr;Gly |
Cys | Ser | Thr | Ser;Val |
Glu | Asp | Trp | Tyr |
Gly | Pro | Tyr | Trp;Phe |
His | Asn;Gln | Val | Ile;Leu |
Ile | Leu,Val |
氨基酸取代、缺失和/或插入可以使用本领域熟知的肽合成技术如固相肽合成法等或通过重组DNA操作轻易地进行。用于操作DNA序列以产生蛋白质的取代、插入或缺失变体的方法是本领域熟知的。例如,用于在DNA的预定位点处产生取代突变的技术是本领域技术人员熟知的并且包括M13诱变法、T7-Gen体外诱变法(USB,Cleveland,OH)、QuickChange位点定向诱变法(Stratagene,San Diego,CA)、PCR介导的位点定向诱变或其他位点定向诱变法。
衍生物
“衍生物”包括这样的肽、寡肽、多肽,其中与天然存在形式蛋白质(如目的蛋白)的氨基酸序列相比较,它们包含非天然存在氨基酸残基对氨基酸的取代或非天然存在氨基酸残基的添加。蛋白质的“衍生物”也包括这样的肽、寡肽、多肽,其中与所述多肽的天然存在形式的氨基酸序列相比,它们包含天然存在的改变(糖基化、酰化、异戊二烯化、磷酸化、肉豆蔻酰化、硫酸化等)的氨基酸残基或非天然存在的改变的氨基酸残基。与衍生出衍生物的氨基酸序列相比较,该衍生物可以也包含与所述氨基酸序列共价或非共价结合的一个或多个非氨基酸取代基或添加物(例如报道分子或其他配体),如所结合旨在促进检测该衍生物的报道分子,和相对于天然存在蛋白质的氨基酸序列而言,包含非天然存在的氨基酸残基。此外,“衍生物”也包括天然存在形式的蛋白质与标签肽如FLAG、HIS6或硫氧还蛋白(对标签肽的综述,参见Terpe,Appl.Microbiol.Biotechnol.60,523-533,2003)的融合物。
直向同源物/旁系同源物
直向同源物和旁系同源物包括用来描述基因祖先关系的进化概念。旁系同源物是相同物种内因祖先基因复制而起源的基因;直向同源物是来自不同生物的因物种形成而起源的基因,并且也衍生于共同的祖先基因。
结构域
术语“结构域”指在进化相关性蛋白质的序列比对结果上的特定位置处保守的一组氨基酸。尽管在其他位置处的氨基酸可以在同源物之间不同,然而在特定位置处高度保守的氨基酸指示在蛋白质结构、稳定性或功能方面很可能是必需的氨基酸。结构域因其在蛋白质同源物家族的比对序列中高程度保守而鉴定,故它们可以用作鉴定物来确定所讨论的任意多肽是否属于先前已鉴定的多肽家族。
基序/共有序列/标签
术语“基序”或“共有序列”或“标签”指在进化相关蛋白质的序列中的短保守区域。基序往往是结构域的高度保守部分,但是也可以仅包括该结构域的部分,或可以位于保守结构域之外(若该基序的全部氨基酸位于定义的结构域之外)。
杂交
如本文中所定义的术语“杂交”是其中基本上同源的互补核苷酸序列相互复性的过程。杂交过程可以完全在溶液中进行,即两种互补性核酸均处在溶液中。杂交过程也可以用固定至基质如磁珠、琼脂糖凝胶(Sepharose)珠或任何其他树脂的互补性核酸之一进行。杂交过程也可以用固定至固体支持物如硝酸纤维素膜或尼龙膜上或通过例如照相平版印刷术固定至例如硅玻璃支持物(后者称作核酸阵列或微阵列或称作核酸芯片)的互补性核酸之一进行。为使杂交发生,核酸分子通常被热变性或化学变性,以将双链解链成两条单链和/或去除来自单链核酸的发夹或其他二级结构。
术语“严格性”指杂交发生的条件。杂交的严格性受诸条件如温度、盐浓度、离子强度和杂交缓冲液组成的影响。通常,将低严格条件选择成在定义的离子强度和pH处,低于特定序列的热解链温度(Tm)约30℃。中等严格条件是当所述温度在Tm以下20℃时,并且高严格条件是当所述温度在Tm以下10℃时。高严格杂交条件一般用于分离与靶核酸序列具有高序列相似性的杂交序列。然而,核酸可以在序列上偏离且依旧编码基本上相同的多肽,原因是遗传密码的简并性。因而,有时候可能需要中等严格杂交条件以鉴定此类核酸分子。
Tm是在定义的离子强度和pH处的下述温度,其中50%的靶序列在所述温度与完全匹配的探针杂交。Tm取决于溶液条件和探针的碱基组成及长度。例如,较长的序列在更高温度上特异性杂交。最大杂交速率从低于Tm约16℃直至32℃获得。杂交溶液中一价阳离子的存在降低了两条核酸链之间的静电排斥作用,因而促进杂交体形成;这种作用对于直到0.4M的钠浓度是显而易见的(对于更高的浓度而言,可以忽略这种作用)。甲酰胺降低DNA-DNA和DNA-RNA双链体的解链温度,每百分数的甲酰胺降低0.6至0.7℃,且添加50%甲酰胺允许在30至45℃杂交,尽管杂交速率会降低。碱基对错配降低杂交速率和双链体的热稳定性。平均且对于大的探针而言,Tm下降约1℃/每%碱基错配。根据杂交体的类型,Tm可以使用以下等式计算:
1)DNA-DNA杂交体(Meinkoth和Wahl,Anal.Biochem.,138:267-284,1984):
Tm=81.5℃+16.6×log10[Na+]a+0.41×%[G/Cb]-500×Lc]-1-0.61×%甲酰胺
2)DNA-RNA杂交体或RNA-RNA杂交体:
Tm=79.8+18.5(log10[Na+]a)+0.58(%G/Cb)+11.8(%G/Cb)2-820/Lc
3)寡DNA杂交体或寡RNAd杂交体:
对少于20个核苷酸而言:Tm=2(ln)
对20-35个核苷酸而言:Tm=22+1.46(ln)
a或者用于其他一价阳离子,但是仅在0.01-0.4M范围内是精确的。
b仅对30%-75%范围内的%GC是精确的。
c L=双链体的碱基对长度。
d Oligo,寡核苷酸;ln,=引物的有效长度=2×(G/C数)+(A/T数)。
可以使用许多已知技术中任意一种技术控制非特异性结合,例如将膜以含有蛋白质的溶液封闭、添加异源RNA、异源DNA和SDS至杂交缓冲液,并且用RNA酶处理。对于非同源性探针,可以通过变换以下条件之一:(i)渐进地降低复性温度(例如从68℃至42℃)或(ii)渐进地降低甲酰胺浓度(例如从50%至0%)进行一系列杂交。技术人员了解可以在杂交期间变更并且会维持或改变所述严格条件的多个参数。
除了杂交条件之外,杂交特异性一般还取决于杂交后洗液的功能。为除去因非特异性杂交引起的背景,用稀释的盐溶液洗涤样品。此类洗液的关键因素包括最终洗涤溶液的离子强度和温度:盐浓度越低且洗涤温度越高,则洗涤的严格性越高。洗涤条件一般在杂交严格性上或低于所述杂交严格性进行。阳性杂交产生至少两倍于背景信号的信号。通常,用于核酸杂交测定法或基因扩增检测方法的适宜严格条件如上所述。也可以选择严格性更高或更低的条件。技术人员了解可以在洗涤期间变更并且会维持或改变所述严格条件的多个参数。
例如,用于长度大于50个核苷酸的DNA杂交体的典型高严格杂交条件包括在65℃于1×SSC中或在42℃于1×SSC和50%甲酰胺中杂交,随后在65℃于0.3×SSC中洗涤。用于长度大于50个核苷酸的DNA杂交体的中等严格杂交条件的实例包括在50℃于4×SSC或在40℃于6×SSC和50%甲酰胺中杂交,随后在50℃于2×SSC中洗涤。杂交体的长度是杂交核酸的预期长度。当序列已知的核酸杂交时,可以通过比对序列并鉴定本文中所述的保守区而确定杂交体长度。1×SSC是0.15M NaCl和15mM柠檬酸钠;杂交溶液和洗涤溶液可以额外地包括5×Denhardt试剂、0.5-1.0%SDS、100μg/ml变性的片段化鲑精DNA、0.5%焦磷酸钠。
出于定义严格性水平的目的,可以参考Sambrook等(2001)Molecular Cloning:a laboratory manual,第三版,Cold Spring HarborLaboratory Press,CSH,New York或参考Current Protocols inMolecular Biology,John Wiley & Sons,N.Y.(1989和年度更新版)。
剪接变体
如本文中所用的术语“剪接变体”包括其中已经切除、取代、置换或添加所选内含子和/或外显子或其中已经缩短或加长内含子的核酸序列的变体。此类变体将是基本上保留蛋白质的生物学活性的一类变体;这可以通过选择性保留蛋白质的功能片段实现。此类剪接变体可以在自然界中找到或可以人工制备。用于预测和分离此类剪接变体的方法是本领域熟知的(见例如Foissac和Schiex(2005)BMC Bioinformatics.6:25)。
等位变体
等位基因或等位变体是给定基因位于相同染色体位置处的备选形式。等位变体包含单核苷酸多态性(SNP)和小的插入/缺失多态性(INDEL)。INDEL的大小通常小于100bp。SNP和INDEL形成大部分生物的天然存在多态性株系中的序列变体的最大集合。
基因改组/定向进化
基因改组或定向进化由反复DNA改组,随后适当筛选和/或选择以产生编码具有改良生物学活性的蛋白质的核酸或其部分的变体而组成(Castle等,(2004)Science 304(5674):1151-4;美国专利5,811,238和6,395,547)。
调节元件/调控序列/启动子
术语“调节元件”、“调控序列”和“启动子”均在本文中可相互交换地使用并且在广泛含义上意指能够实现与它们相连接的序列表达的调节性核酸序列。术语“启动子”一般指位于基因转录起点上游并参与识别和结合RNA聚合酶和其他蛋白质,因而指导有效链接的核酸转录的核酸调控序列。前述术语包括从经典真核基因组基因(包括对于精确转录启动所需的TATA框,具有或没有CCAAT框序列)衍生的转录调节序列和应答发育性刺激和/或外部刺激或以组织特异性方式而改变基因表达的其他调节元件(即上游激活序列、增强子和沉默子)。本术语还包括经典原核基因的转录调节序列,在此情况下它可以包括一个-35框序列和/或一个-10框转录调节序列。术语“调节元件”也包含赋予、激活或增加核酸分子在细胞、组织或器官中表达的人工融合分子或衍生物。
“植物启动子”包含介导植物细胞中编码序列节段表达的调节元件。因此,植物启动子不必须是植物来源的,但可以源自病毒或微生物,例如来自侵袭植物细胞的病毒。“植物启动子”也可以源自植物细胞,例如来自用在本发明方法中待表达并在本文中描述的核酸序列转化的植物。这也适用于其他“植物”调节信号,如“植物”终止子。用于本发明方法中的核苷酸序列上游的启动子可以通过一个或多个核苷酸取代、插入和/或缺失进行修饰,但不影响启动子、可读框(ORF)或3’调节区如终止子或远离ORF存在的其他3’调节区的功能性或活性。还有可能所述启动子的活性因修饰其序列或它们被更活跃的启动子、甚至来自异源生物的启动子彻底取代而提高。为了在植物中表达,如上所述,核酸分子必须有效地连接至或包含在正确的时间点并以所需空间表达模式表达基因的合适启动子。
为鉴定功能性等同启动子,候选启动子的启动子强度和/或表达模式可以例如通过将此启动子有效链接至报道基因并分析该报道基因在植物的多种组织中的表达水平和模式进行分析。合适的熟知报道基因包括例如β-葡糖醛酸酶或β-半乳糖苷酶。启动子活性通过测量β-葡糖醛酸酶或β-半乳糖苷酶的酶活性进行分析。启动子强度和/或表达模式可以随后与参考启动子(如在本发明方法中使用的一种启动子)的启动子强度和/或表达模式比较。备选地,启动子强度可以使用本领域已知方法如RNA印迹法及放射自显影图的密度计分析法、定量实时PCR或RT-PCR(Heid等,1996Genome Methods 6:986-994),通过量化mRNA水平或通过将本发明方法中所用核酸的mRNA水平与持家基因(如18S rRNA)的mRNA水平比较进行分析。通常“弱启动子”意指驱动编码序列在低水平表达的启动子。“低水平”意指在每个细胞约1/10,000转录物至约1/100,000转录物、至约1/500,0000转录物的水平上。相反,“强启动子”驱动编码序列在高水平或以每个细胞约1/10转录物至约1/100转录物、至约1/1000转录物表达。一般而言,“中等强度启动子”意指驱动编码序列以在一切情况下低于受35S CaMV启动子控制时所获得水平的水平表达的启动子。
有效连接
如本文中所用的术语“有效连接”指启动子序列与目的基因之间的功能连接,从而该启动子序列能够启动该目的基因转录。
组成型启动子
“组成型启动子”指在生长和发育的大部分期间但不是必需在全部期间,以及在大多数环境条件下,在至少一种细胞、组织或器官中有转录活性的启动子。下表2a给出组成型启动子的实例。
表2a:组成型启动子的实例
基因来源 | 参考文献 |
肌动蛋白 | McElroy等,Plant Cell,2:163-171,1990 |
HMGP | WO 2004/070039 |
CAMV35S | Odell等,Nature,313:810-812,1985 |
CaMV 19S | Nilsson等,Physiol.Plant.100:456-462,1997 |
GOS2 | de Pater等,Plant J Nov;2(6):837-44,1992,WO 2004/065596 |
遍在蛋白 | Christensen等,Plant Mol.Biol.18:675-689,1992 |
稻亲环蛋白 | Buchholz等,Plant Mol Biol.25(5):837-43,1994 |
玉米H3组蛋白 | Lepetit等,Mol.Gen.Genet.231:276-285,1992 |
苜蓿H3组蛋白 | Wu等,Plant Mol.Biol.11:641-649,1988 |
肌动蛋白2 | An等,Plant J.10(1);107-121,1996 |
34S FMV | Sanger等,Plant.Mol.Biol.,14,1990:433-443 |
核酮糖二磷酸羧化酶-加氧酶小亚基 | US 4,962,028 |
OCS | Leisner(1988)Proc Natl Acad Sci USA 85(5):2553 |
SAD1 | Jain等,Crop Science,39(6),1999:1696 |
SAD2 | Jain等,Crop Science,39(6),1999:1696 |
nos | Shaw等,(1984)Nucleic Acids Res.12(20):7831-7846 |
V-ATP酶 | WO 01/14572 |
超级启动子(Super promoter) | WO 95/14098 |
G框蛋白质 | WO 94/12015 |
遍在启动子
遍在启动子是在生物的全部组织或细胞中基本上有活性的。
发育调节型启动子
发育调节型启动子在某些发育阶段期间或在经历发育变化的植物的部分中有活性。
诱导型启动子
诱导型启动子在应答化学刺激(综述见Gatz 1997,Annu.Rev.PlantPhysiol.Plant Mol.Biol.,48:89-108)、环境刺激或物理刺激时具有诱导或提高的转录启动作用,或可以是“胁迫诱导的”,即当植物暴露于多种胁迫条件时其激活,或是“病原体诱导的”,即当植物暴露于多种病原体时其激活。
器官特异性/组织特异性启动子
器官特异性或组织特异性启动子是能够偏好地启动某些器官或组织如叶、根、种子组织等中转录的启动子。例如,“根特异性启动子”是这样的启动子,该启动子优势地在植物根中具有转录活性,基本上在植物的任何其他部分中无活性,尽管在该植物的这些其他部分中仍允许任意泄露表达。能够仅在某些细胞中启动转录的启动子在本文中称作“细胞特异性的”。
根特异性启动子的实例列于下表2b中。
表2b:根特异性启动子的实例
基因来源 | 参考文献 |
RCc3 | Plant Mol Biol.1995Jan;27(2):237-48 |
拟南芥属PHT1 | Kovama等,2005;Mudge等(2002,Plant J.31:341) |
苜蓿属(Medicago)磷酸转运蛋白 | Xiao等,2006 |
拟南芥属Pyk10 | Nitz等(2001)Plant Sci 161(2):337-346 |
可在根表达的基因 | Tingey等,EMBO J.6:1,1987. |
烟草生长素诱导型基因 | Van der Zaal等,Plant Mol.Biol.16,983,1991. |
β-微管蛋白 | Oppenheimer,等,Gene 63:87,1988. |
烟草根特异性基因 | Conkling,等,Plant Physiol.93:1203,1990. |
欧洲油菜(B.napus)G1-3b基因 | 美国专利No.5,401,836 |
SbPRP1 | Suzuki等,Plant Mol.Biol.21:109-119,1993. |
LRX1 | Baumberger等2001,Genes&Dev.15:1128 |
BTG-26欧洲油菜(Brassicanapus) | US 20050044585 |
LeAMT1(番茄) | Lauter等(1996,PNAS 3:8139) |
The LeNRT1-1(番茄) | Lauter等(1996,PNAS 3:8139) |
I类patatin基因(马铃薯) | Liu等,Plant Mol.Biol.153:386-395,1991. |
KDC1(胡萝卜(Daucus carota)) | Downey等(2000,J.Biol.Chem.275:39420) |
TobRB7基因 | W Song(1997)PhD Thesis,North Carolina StateUniversity,Raleigh,NC USA |
OsRAB5a(稻) | Wang等2002,Plant Sci.163:273 |
ALF5(拟南芥属) | Diener等(2001,Plant Cell 13:1625) |
NRT2;1Np(白花丹叶烟草(N.plumbaginifolia)) | Quesada等(1997,Plant Mol.Biol.34:265) |
种子特异性启动子是能够在种子组织中优势地具有转录活性的启动子,但无需排他性地在种子组织中有转录活性(在泄露表达的情况下)。种子特异性启动子可以在种子发育期间和/或萌发期间有活性。种子特异性启动子可以是胚乳和/或糊粉和/或胚特异性。种子特异性启动子的实例在下表2c至2f中给出。种子特异性启动子的其他例子在QingQu和Takaiwa(Plant Biotechnol.J.2,113-125,2004)中给出,所述文献的公开内容如完整所述那样通过引用方式并入本文。
表2c:种子特异性启动子的实例
基因来源 | 参考文献 |
种子特异性基因 | Simon等,Plant Mol.Biol.5:191,1985; |
Scofield等,J.Biol.Chem.262:12202,1987.; | |
Baszczynski等,Plant Mol.Biol.14:633,1990. | |
巴西坚果(Brazil Nut)白蛋白 | Pearson等,Plant Mol.Biol.18:235-245,1992. |
豆球蛋白 | Ellis等,Plant Mol.Biol.10:203-214,1988. |
谷蛋白(稻) | Takaiwa等,Mol.Gen.Genet.208:15-22,1986; |
Takaiwa等,FEBS Letts.221:43-47,1987. | |
玉米醇溶蛋白 | Matzke等,Plant Mol Biol,14(3):323-321990 |
NapA | Stalberg等,Planta 199:515-519,1996. |
小麦LMW和HMW麦谷蛋白-1 | Mol Gen Genet 216:81-90,1989;NAR 17:461-2,1989 |
小麦SPA | Albani等,Plant Cell,9:171-184,1997 |
小麦α、β、γ-麦醇溶蛋白 | EMBO J.3:1409-15,1984 |
大麦Itr1启动子 | Diaz等,(1995)Mol Gen Genet 248(5):592-8 |
大麦B1、C、D大麦醇溶蛋白 | Theor Appl Gen 98:1253-62,1999;Plant J 4:343-55,1993;Mol Gen Genet 250:750-60,1996 |
大麦DOF | Mena等,The Plant Journal,116(1):53-62,1998 |
blz2 | EP99106056.7 |
合成的启动子 | Vicente-Carbajosa等,Plant J.13:629-640,1998. |
稻谷醇溶蛋白NRP33 | Wu等,Plant Cell Physiology 39(8)885-889,1998 |
稻α-球蛋白Glb-1 | Wu等,Plant Cell Physiology 39(8)885-889,1998 |
稻OSH1 | Sato等,Proc.Natl.Acad.Sci.USA,93:8117-8122,1996 |
稻α-球蛋白REB/OHP-1 | Nakase等,Plant Mol.Biol.33:513-522,1997 |
稻ADP-葡萄糖焦磷酸酶 | Trans Res 6:157-68,1997 |
玉米ESR基因家族 | Plant J 12:235-46,1997 |
高粱α-高粱醇溶蛋白 | DeRose等,Plant Mol.Biol 32:1029-35,1996 |
KNOX | Postma-Haarsma等,Plant Mol.Biol.39:257-71,1999 |
稻油质蛋白 | Wu等,J.Biochem.123:386,1998 |
向日葵油质蛋白 | Cummins等,Plant Mol.Biol.19:873-876,1992 |
PRO0117,假定的稻40S核糖体蛋白 | WO 2004/070039 |
PRO0136,稻丙氨酸氨基转移酶 | 未公开 |
PRO0147,胰蛋白酶抑制剂ITR1(大麦) | 未公开 |
PRO0151,稻WSI18 | WO 2004/070039 |
PRO0175,稻RAB21 | WO 2004/070039 |
PRO005 | WO 2004/070039 |
PRO0095 | WO 2004/070039 |
α-淀粉酶(Amy32b) | Lanahan等,Plant Cell 4:203-211,1992;Skriver等,Proc Natl Acad Sci USA 88:7266-7270,1991 |
组织蛋白酶β样基因 | Cejudo等,Plant Mol Biol 20:849-856,1992 |
大麦Ltp2 | Kalla等,Plant J.6:849-60,1994 |
Chi26 | Leah等,Plant J.4:579-89,1994 |
玉米B-Peru | Selinger等,Genetics 149;1125-38,1998 |
表2d:胚乳特异性启动子的实例
基因来源 | 参考文献 |
谷蛋白(稻) | Takaiwa等,(1986)Mol Gen Genet 208:15-22Takaiwa等,(1987)FEBS Letts.221:43-47 |
玉米醇溶蛋白 | Matzke等,(1990)Plant Mol Biol 14(3):323-32 |
小麦LMW和HMW麦谷蛋白-1 | Colot等,(1989)Mol Gen Genet 216:81-90Anderson等,(1989)NAR 17:461-2 |
小麦SPA | Albani等,(1997)Plant Cell 9:171-184 |
小麦麦醇溶蛋白 | Rafalski等,(1984)EMBO 3:1409-15 |
大麦Itr1启动子 | Diaz等,(1995)Mol Gen Genet 248(5):592-8 |
大麦B1,C,D,大麦醇溶蛋白 | Cho等,(1999)Theor Appl Genet 98:1253-62Muller等,(1993)Plant J 4:343-55Sorenson等,(1996)Mol Gen Genet 250:750-60 |
大麦DOF | Mena等,(1998)Plant J 116(1):53-62 |
blz2 | Onate等,(1999)J Biol Chem 274(14):9175-82 |
合成启动子 | Vicente-Carbajosa等,(1998)Plant J 13:629-640 |
稻谷醇溶蛋白NRP33 | Wu等,(1998)Plant Cell Physiol 39(8)885-889 |
稻球蛋白Glb-1 | Wu等,(1998)Plant Cell Physiol 39(8)885-889 |
稻球蛋白REB/OHP-1 | Nakase等,(1997)Plant Molec Biol 33:513-522 |
稻ADP-葡萄糖焦磷酸化酶 | Russell等,(1997)Trans Res 6:157-68 |
玉米ESR基因家族 | Opsahl-Ferstad等,(1997)Plant J 12:235-46 |
高粱醇溶蛋白 | DeRose等,(1996)Plant Mol Biol 32:1029-35 |
表2e:胚特异性启动子的实例
基因来源 | 参考文献 |
稻OSH1 | Sato等,Proc.Natl.Acad.Sci.USA,93:8117-8122,1996 |
KNOX | Postma-Haarsma等,Plant Mol.Biol.39:257-71,1999 |
PRO0151 | WO 2004/070039 |
PRO0175 | WO 2004/070039 |
PRO005 | WO 2004/070039 |
PRO0095 | WO 2004/070039 |
表2f:糊粉特异性启动子的实例
基因来源 | 参考文献 |
α-淀粉酶(Amy32b) | Lanahan等,Plant Cell 4:203-211,1992;Skriver等,Proc Natl Acad Sci USA 88:7266-7270,1991 |
组织蛋白酶β-样基因 | Cejudo等,Plant Mol Biol 20:849-856,1992 |
大麦Ltp2 | Kalla等,Plant J.6:849-60,1994 |
Chi26 | Leah等,Plant J.4:579-89,1994 |
玉米B-Peru | Selinger等,Genetics 149;1125-38,1998 |
如本文中所定义的绿色组织特异性启动子是优势地在绿色组织中具有转录活性的启动子,在植物的任何其他部分内基本上无活性,尽管在该植物的这些其他部分中仍允许任意泄露表达。
可以用来实施本发明方法的绿色组织特异性启动子的实例示于下表2g中。
表2g:绿色组织特异性启动子的实例
基因 | 表达 | 参考文献 |
玉米正磷酸二激酶 | 叶特异性 | Fukavama等,2001 |
玉米磷酸烯醇式丙酮酸羧化酶 | 叶特异性 | Kausch等,2001 |
稻磷酸烯醇式丙酮酸羧化酶 | 叶特异性 | Liu等,2003 |
稻核酮糖二磷酸羧化酶-加氧酶小亚基 | 叶特异性 | Nomura等,2000 |
稻β扩展蛋白EXBP9 | 枝条特异性 | WO 2004/070039 |
木豆(Pigeonpea)核酮糖二磷酸羧化酶-加氧酶小亚基 | 叶特异性 | Panguluri等,2005 |
豌豆RBCS3A | 叶特异性 |
组织特异性启动子的另一个例子是分生组织特异性启动子,其优势地在分生组织中具有转录活性,在植物的任何其他部分内基本上无活性,尽管在该植物的这些其他部分中仍允许任意泄露表达。可以用来实施本发明方法的绿色分生组织特异性启动子的实例示于下表2h中。
表2h:分生组织特异性启动子的实例
基因来源 | 表达模式 | 参考文献 |
稻OSH1 | 从胚球期至籽苗期的枝条顶端分生组织 | Sato等,(1996)Proc.Natl.Acad.Sci.USA,93:8117-8122 |
稻金属硫蛋白 | 分生组织特异性 | BAD87835.1 |
WAK1和WAK2 | 枝条和根顶端分生组织,和在扩展的叶和萼片中 | Wagner和Kohorn(2001)Plant Cell13(2):303-318 |
终止子
术语“终止子”包括作为转录单元末端处DNA序列的调控序列,所述的DNA序列产生初级转录物的3’加工和多腺苷酸化及转录终止的信号。终止子可以从天然基因、从多种其他植物基因或从T-DNA衍生。待添加的终止子可以从例如胭脂碱合酶或章鱼碱合酶基因或备选地从另一种植物基因或较次优选地从任何其他真核基因衍生。
调节
就表达或基因表达而言,术语“调节”意指这样的过程,其中与对照植物相比,表达水平因该基因表达而改变,所述表达水平提高或降低。原始、未调节的表达可以是结构性RNA(rRNA、tRNA)或mRNA的任何类型的表达,随后是翻译。术语“调节活性”应当意指本发明核酸序列或所编码蛋白质的表达的任何改变,这引起植物产量提高和/或生长增加。
表达
术语“表达”或“基因表达”意指某个特定基因或多个特定基因或特定基因构建体的转录。术语“表达”或“基因表达”尤其意指某个基因或某些基因或基因构建体转录成结构性RNA(rRNA、tRNA)或mRNA,所述RNA随后翻译成或不翻译成蛋白质。该过程包括DNA的转录和所得mRNA产物的加工。
增加的表达/过量表达
如本文中所用的术语“增加的表达”或“过量表达”意指相对于原有野生型表达水平为额外的任何形式的表达。
在本领域内充分报道了用于提高基因或基因产物表达的方法并且这些方法包括例如由适宜启动子驱动的过量表达、使用转录增强子或翻译增强子。充当启动子或增强子元件的分离核酸可以导入多核苷酸的非异源形式的适宜位置(一般在上游)中,从而上调编码目的多肽的核酸表达。例如,内源性启动子可以在体内通过突变、缺失和/或取代加以改变(见Kmiec,US 5,565,350;Zarling等,WO9322443),或可以将分离的启动子以相对于本发明基因的恰当方向及距离导入植物细胞,从而控制该基因的表达。
若需要多肽表达,通常希望的是在多核苷酸编码区的3’末端处包括多腺苷化区域。该多腺苷酸化区域可以从天然基因、从多种其他植物基因或从T-DNA衍生。待添加的3’末端序列可以从例如胭脂碱合酶或章鱼碱合酶基因或备选地从另一种植物基因或较不优选地从任何其他真核基因衍生。
内含子序列也可以添加至5’非翻译区(UTR)或部分编码序列的编码序列以提高细胞质中聚集的成熟信使的量。已经显示在植物和动物表达构建体的转录单位中包含可剪接内含子提高了mRNA水平及蛋白质水平上的基因表达高达1000倍(Buchman和Berg(1988)Mol.Cellbiol.8:4395-4405;Callis等(1987)Gens Dev 1:1183-1200)。此种内含子增强基因表达的作用一般在所述内含子置于转录单位的5’末端附近时最强烈。玉米内含子Adh1-S内含子1、2和6、Bronze-1内含子的用途是本领域已知的。对于总体信息,见:The Maize Handbook,第116章,Freeling和Walbot编,Springer,N.Y.(1994)。
内源基因
本文中对“内源”基因的称谓不仅仅指如植物中以其天然形式(即没有人类任何干预)存在的所讨论基因,还指处于分离形式下的随后(再)导入植物(转基因)的相同基因(或基本上同源的核酸/基因)。例如,含有这种转基因的转基因植物可以遭遇转基因表达的相当大程度地降低和/或内源基因表达的实质降低。分离的基因可以从生物分离或可以是人造的,例如通过化学合成法。
降低的表达
本文中提及的“降低的表达”或“降低或基本消除表达”意指内源基因表达和/或多肽水平和/或多肽活性相对于对照植物的下降。与对照植物相比较,所述降低或基本上消除以增加的优选顺序是至少10%、20%、30%、40%或50%、60%、70%、80%、85%、90%或95%、96%、97%、98%、99%或更多降低。增加表达的方法是本领域公知的,且例如本领域技术人员能够容易地采用用于沉默的方法,从而通过利用合适启动子实现在完整植物中或其部分中降低内源基因的表达。
为了降低或基本消除植物中内源基因的表达,需要核酸序列的基本上连续的核苷酸的足够长度。为进行基因沉默,该长度可以短至20、19、18、17、16、15、14、13、12、11、10个或更少核苷酸,或者该长度可以长至整个基因(包括部分或完整的5’和/或3’UTR)。基本上连续的核苷酸片段可以从编码目的蛋白的核酸(靶基因)衍生,或从能够编码目的蛋白的直向同源物、旁系同源物或同源物的任何核酸衍生。优选地,基本上连续的核苷酸的片段能够与靶基因(有义链或反义链)形成氢键,更优选地,基本上连续的核苷酸片段以增加的优选顺序与靶基因(有义链或反义链)具有50%、60%、70%、80%、85%、90%、95%、96%、97%、98%、99%、100%的序列同一性。编码(功能性)多肽的核酸序列不是本文中所讨论用于降低或基本消除内源基因表达的多种方法的前提。
可以使用常规工具和技术完成表达的这种降低或基本消除。用于降低或基本消除内源基因表达的优选方法是在植物中引入并表达基因构建体,其中将核酸(在此情况下,从目的基因衍生的,或从能够编码任一目的蛋白的直向同源物、旁系同源物或同源物的任何核酸中衍生的一段基本上连续的核苷酸)克隆至所述基因构建体,(部分或完全地)作为被间隔序列(非编码性DNA)隔开的反向重复序列。
在这种优选的方法中,使用核酸或其部分(在此情况下,所述部分是从目的基因衍生的,或从能够编码目的蛋白的直向同源物、旁系同源物或同源物的任何核酸中衍生的一段基本上连续的核苷酸)的反向重复序列(其优选能够形成发夹结构),通过RNA介导的沉默作用降低或基本上消除内源基因的表达。在包含调控序列的表达载体中克隆该反向重复序列。非编码性DNA核酸序列(间隔序列,例如基质附着区片段(MAR)、内含子、多接头等)位于形成所述反向重复序列的两个反向核酸之间。在反向重复序列转录后,形成具有(部分或完全)自我互补性结构的嵌合RNA。这种双链RNA结构称作发夹RNA(hpRNA)。hpRNA由植物加工成siRNA,该siRNA被掺入RNA诱导的沉默复合体(RISC)。该RISC进一步切开所述mRNA转录物,从而相当大程度地降低待翻译成多肽的mRNA转录物的数目。对于其他一般细节,参见例如Grierson等(1998)WO 98/53083;Waterhouse等(1999)WO 99/53050。
本发明方法的实施不取决于在植物中引入并表达将所述核酸作为反向重复序列克隆到其中的基因构建体,不过可以使用几种熟知“基因沉默”方法中任何一种或多种方法来实现相同效果。
用于降低内源基因表达的一种这样的方法是RNA介导的基因表达沉默(下调)。在这种情况下,沉默作用由植物中与内源性靶基因实质相似的双链RNA序列(dsRNA)触发。这种dsRNA进一步被植物加工成约20个至约26个核苷酸的所谓短干扰RNA(siRNA)。所述siRNA被掺入RNA诱导的沉默复合体(RISC),其中所述RISC切割内源靶基因的mRNA转录物,从而相当大程度地将降低待翻译成多肽的mRNA转录物的数目。优选地,所述双链RNA序列与靶基因对应。
RNA沉默方法的另一个例子涉及将核酸序列或其部分(在此情况下是从目的基因衍生的,或从能够编码目的蛋白的直向同源物、旁系同源物或同源物的任何核酸中衍生的一段基本上连续的核苷酸)以有义方向导入植物。“有义方向”是指与自身mRNA转录物同源的DNA序列。因而将所述核酸序列的至少一个拷贝导入植物。这个额外核酸序列会降低内源基因表达,从而产生已知为共抑制作用的现象。将一个核酸序列的几个额外拷贝导入植物时,基因表达的降低将更明显,因为高转录物水平与触发共抑制作用之间存在正相关。
RNA沉默方法的另一个例子涉及使用反义核酸序列。“反义”核酸序列包含与编码蛋白质的“有义”核酸序列互补,即与双链cDNA分子的编码链互补,或与mRNA转录物序列互补的核苷酸序列。反义核酸序列优选地互补于待沉默的内源基因。这种互补性可以存在于基因的“编码区”中和/或其“非编码区”中。术语“编码区”指包含被翻译成氨基酸残基的密码子的核苷酸序列的区域。术语“非编码区”指分布在编码区侧翼的被转录但不翻译成氨基酸的5’和3’序列(也称作5’和3’非翻译区)。
反义核酸序列可以根据Watson和Crick碱基配对规则设计。反义核酸序列可以互补于整个核酸序列(在此情况下是从目的基因衍生的,或从能够编码目的蛋白的直向同源物、旁系同源物或同源物的任何核酸中衍生的一段基本上连续的核苷酸),不过也可以是仅对所述核酸序列的一部分(包括mRNA 5’和3’UTR)反义的寡核苷酸。例如,反义寡核苷酸序列可以互补于编码多肽的mRNA转录物的翻译起点周围的区域。合适反义寡核苷酸序列的长度是本领域已知的并且可以从约50、45、40、35、30、25、20、15或10个核苷酸或更小的核苷酸长度开始。本发明的反义核酸序列可以使用化学合成反应和酶连接反应,利用本领域已知的方法构建。例如,反义核酸序列(例如反义寡核苷酸序列)可以使用天然存在核苷酸或以多种方式修饰的核苷酸化学地合成,其中所述的修饰核苷酸设计旨在增加分子的生物学稳定性或增加反义与有义核酸序列之间所形成的双链体的物理稳定性,例如,可以使用硫代磷酸酯衍生物和吖啶取代的核苷酸。可以用来产生反义核酸序列的修饰核苷酸的实例是本领域熟知的。已知的核苷酸修饰包括甲基化、环化和‘加帽’及用类似物(如肌苷)取代一个或多个天然存在核苷酸。对核苷酸的其他修饰作用是本领域熟知的。
反义核酸序列可以使用表达载体以生物学方式产生,其中一种核酸序列已经以反义方向亚克隆(即从插入的核酸转录出的RNA会对目的靶核酸为反义方向)到所述表达载体中。优选地,植物中反义核酸序列的产生借助稳定整合的核酸构建体进行,其中所述的核酸构建体包含启动子、有效链接的反义寡核苷酸和终止子。
用于本发明方法中沉默作用的核酸分子(无论被导入植物中或原位(in situ)地产生)与mRNA转录物和/或编码多肽的基因组DNA杂交或结合以因而抑制蛋白质的表达,例如通过抑制转录和/或翻译做到这一点。杂交可以因形成稳定双链体的常规核苷酸互补性引起,或例如,在与DNA双链体结合的反义核酸序列的情况下,因双螺旋大沟内的特异性相互作用引起。反义核酸序列可以通过在特定组织部位转化或直接注射导入植物。备选地,反义核酸序列可以被修饰以靶向所选的细胞并且随后全身性施用。例如,对于全身性施用,可以修饰反义核酸序列,从而它们与表达在所选细胞表面上的受体或抗原特异性地结合,例如通过将所述反义核酸序列连接至与细胞表面受体或抗原结合的肽或抗体连接而做到这一点。反义核酸序列也可以使用本文中所述的载体递送至细胞。
根据另一方面,反义核酸序列是α-端基异构核酸序列。α-端基异构核酸序列与互补RNA形成特定的双链杂交体,在所述双链杂交体中与常见的b-单元相反,所述链彼此平行(Gaultier等(1987)Nucl Ac Res15:6625-6641)。反义核酸序列也可以包含2’-O-甲基核糖核苷酸(Inoue等(1987)Nucl Ac Res 15,6131-6148)或嵌合RNA-DNA类似物(Inoue等(1987)FEBS Lett.215,327-330)。
内源基因表达的降低或基本上消除也可以使用核酶进行。核酶是具有核糖核酸酶活性的催化性RNA分子,能够切割与之具有互补区域的单链核酸序列,如mRNA。因此,核酶(例如锤头状核酶(在Haselhoff和Gerlach(1988)Nature 334,585-591中描述)可以用来催化地切割编码多肽的mRNA转录物,因而相当大程度地降低待翻译成多肽的mRNA转录物的数目。可以设计对核酸序列具有专一性的核酶(参见例如:Cech等美国专利号4,987,071;和Cech等美国专利号5,116,742)。备选地,与核酸序列相对应的mRNA转录物可以用来从RNA分子的汇集物中选出具有特定核糖核酸酶活性的催化性RNA(Bartel和Szostak(1993)Science 261,1411-1418)。核酶在植物中用于基因沉默的用途是本领域已知的(例如Atkins等(1994)WO 94/00012;Lenne等(1995)WO95/03404;Lutziger等(2000)WO 00/00619;Prinsen等(1997)WO97/13865和Scott等(1997)WO 97/38116)。
基因沉默也可以通过插入诱变(例如T-DNA插入或转座子插入)或通过如Angell和Baulcombe((1999)Plant J.20(3):357-62)、(AmpliconVIGS WO 98/36083)或Baulcombe(WO 99/15682)及其他人描述的策略实现。
如果内源基因中存在突变和/或在随后导入植物的分离基因/核酸中存在突变,基因沉默也可能发生。所述降低或基本上消除可以由无功能的多肽引起。例如,该多肽可以与多种相互作用的蛋白质结合;一种或多种突变和/或截短作用因而可以产生仍能够结合相互作用的蛋白质(如受体蛋白)但不能展示正常功能的多肽(如信号传导配体)。
基因沉默的另一种方法是瞄准互补于基因调节区(例如启动子和/或增强子)的核酸序列以形成阻止靶细胞中基因转录的三重螺旋结构。参见Helene,C.,Anticancer Drug Res.6,569-84,1991;Helene等,Ann.N.Y.Acad.Sci.660,27-361992;和Maher,L.J.Bioassays 14,807-15,1992。
技术人员会熟知其他方法,如使用针对内源多肽的抗体以抑制该多肽在植物中(in planta)的功能,或干扰涉及某多肽的信号传导途径。特别地,可以考虑人造分子可能用于抑制靶多肽的生物学功能,或用于干扰涉及所述靶多肽的信号传导途径。
备选地,可以建立筛选程序以鉴定植物群体中基因的天然变体,其中所述的变体编码具有降低的活性的多肽。也可以使用此类天然变体,例如来进行同源重组。
人工和/或天然的微RNA(miRNA)可以用来敲除基因表达和/或mRNA翻译。内源miRNA是通常19-24个核苷酸长度的单链小RNA。它们主要发挥调节基因表达和/或mRNA翻译的功能。大多数的植物微RNA(miRNA)与其靶序列具有完全或接近完全的互补性。然而,存在具有多达5个错配的天然靶。它们从具有特征性折返结构的较长非编码性RNA由Dicer家族的双链特异性RNA酶加工得来。加工后,它们通过与RNA诱导的沉默复合体(RISC)的主要组分-Argonaute蛋白结合被掺入该复合体。miRNA充当RISC的特异性组分,因为它们与胞浆中的靶核酸(大多是mRNA)发生碱基配对。后续调节事件包括靶mRNA切割和摧毁和/或翻译抑制。miRNA过量表达的影响因此往往反映为靶基因的mRNA水平降低。
可以专门地遗传工程化一般21个核苷酸长度的人工微RNA(amiRNA)以负向地调节单个或多个目的基因的基因表达。选择植物的微RNA靶的决定因素是本领域熟知的。用于靶识别的经验参数已经被定义并可以用来辅助具体amiRNA的设计(Schwab等,Dev.Cell 8,517-527,2005)。用于设计并产生amiRNA及其前体的便利工具也是公众可获得的(Schwab等,Plant Cell 18,1121-33,2006)。
为了最佳性能,用于降低植物中内源基因表达的基因沉默技术需要使用来自单子叶植物的核酸序列转化单子叶植物,并使用来自双子叶植物的核酸序列转化双子叶植物。优选地,将来自任意的给定植物物种的核酸序列导入相同的物种。例如,将来自稻的核酸序列转化到稻植物中。然而,不绝对要求待导入的核酸序列来自与待导入该核酸序列的植物相同的植物物种。只要内源靶基因与待导入的核酸之间存在实质同源性即可。
上文描述用于降低或基本上消除植物中内源基因表达的多种方法的实例。例如,本领域技术人员会轻易地能够调整用于沉默的前述方法,从而通过利用合适启动子实现在完整植物中或其部分中降低内源基因的表达。
选择性标记(基因)/报道基因
“选择性标记”、“选择性标记基因”或“报道基因”包括向细胞赋予表型的任意基因,其中在所述细胞中表达所述“选择性标记”、“选择性标记基因”或“报道基因”以促进鉴定和/或选择用本发明核酸构建体转染或转化的细胞。这些标记基因能够借助一系列不同原理而鉴定核酸分子的成功转移。合适的标记可以选自赋予抗生素抗性或除草剂抗性、导入新代谢性状或允许目视选择的标记。选择性标记基因的实例包括赋予抗生素抗性的基因(如使新霉素和卡那霉素磷酸化的nptII或使潮霉素磷酸化的hpt或赋予针对例如博来霉素、链霉素、四环素、氯霉素、氨苄青霉素、庆大霉素、遗传霉素(Geneticin)(G418)、壮观霉素或杀稻瘟菌素的抗性的基因)、赋予除草剂抗性的基因(例如提供抗性的bar;提供草甘膦抗性的aroA或gox或赋予针对例如咪唑啉酮、膦丝菌素或磺脲类的抗性的基因)或提供代谢性状的基因(如允许植物使用甘露糖作为唯一碳源的manA,或利用木糖的木糖异构酶,或抗营养性标记如2-脱氧葡萄糖抗性)。目视标记基因的表达导致颜色(例如β-葡糖醛酸酶、GUS或β-半乳糖苷酶与其有色底物例如X-Gal)、发光(如萤光素/萤光素酶系统)或荧光(绿色荧光蛋白GFP和其衍生物)的形成。这个名单仅代表少数的可能标记。技术人员熟悉此类标记。取决于生物和选择方法,优选不同的标记。
已知当核酸稳定或瞬时地整合至植物细胞时,仅少数细胞摄取外来DNA,并且根据需要,将外来DNA整合至细胞基因组中,这取决于所用的表达载体和所用的转染技术。为鉴定并选择这些整合体,通常将编码选择性标记的基因(如上文所述的基因)连同目的基因一起导入宿主细胞。这些标记可以在这些基因例如通过常规方法缺失而无功能的突变体中使用。此外,编码选择性标记的核酸分子可以在包含编码本发明多肽或在本发明方法中所用多肽的序列的相同载体上,或在独立的载体上导入宿主细胞。已经用所导入核酸稳定转染的细胞可以例如通过选择作用鉴定(例如具有整合的选择性标记的细胞存活而其他细胞死亡)。一旦不再需要所述标记基因时,可以从转基因细胞中移除或切除它们。用于标记移除的技术是本领域已知的,有用的技术在上文定义部分中描述。
因为一旦已经成功地导入所述标记基因、尤其抗生素抗性基因和除草剂抗性基因,则这些核酸是转基因宿主细胞中不再需要或不想要的,因此用于导入核酸的本发明方法有利地使用能够移除或切除这些标记基因的技术。一种这样的方法是所谓共转化法。共转化法同时使用两种载体以转化,一种载体携带本发明的核酸而第二种载体携带标记基因。大比例的转化体接受或在植物情况下包含(多达40%或更多的转化体)这两种载体。在用农杆菌(Agrobacterium)转化的情况下,转化体通常仅接受载体的一部分,即侧翼存在T-DNA的序列,该序列通常代表表达盒。标记基因随后可以通过开展杂交从转化植物中移除。在另一种方法中,整合至转座子的标记基因与想要的核酸一起用于转化(称作Ac/Ds技术)。转化体可以与转座酶来源物杂交,或转化体用引起转座酶表达的核酸构建体瞬时或稳定转化。在一些情况下(大约10%),一旦转化已经成功发生,则转座子从宿主细胞的基因组跳出并丢失。在其他许多情况下,转座子跳到一个不同位置。在这些情况下,标记基因必须通过开展杂交予以消除。在微生物学中,开发了有可能或促进检测这类事件的技术。又一种有利方法依赖于所谓重组系统;所述方法的优势在于杂交消除作用可以用该重组系统实行。最知名的该类型系统称作Cre/lox系统。Cre1是移除位于loxP序列之间序列的重组酶。若所述标记基因整合于loxP序列之间,一旦转化已经成功发生,则它因重组酶表达而被移除。其他重组系统是HIN/HIX、FLP/FRT和REP/STB系统(Tribble等,J.Biol.Chem.,275,2000:22255-22267;Velmurugan等,J.Cell Biol.,149,2000:553-566)。位点特异性地整合本发明核酸序列至植物基因组是可能的。自然,这些方法也可以应用于微生物如酵母、真菌或细菌。
转基因的/转基因/重组
为本发明的目的,“转基因的”、“转基因”或“重组”例如就核酸序列而言,意指包含所述核酸序列的表达盒、基因构建体或载体,或用本发明核酸序列、表达盒或载体转化的生物,这些构建体均通过重组方法产生,其中
(a)编码用于本发明方法中的蛋白质的核酸序列,或
(b)与本发明核酸序列有效链接的基因调控序列,例如启动子,或
(c)a)和b)
并不位于它们的天然遗传环境中或已经通过重组方法被修饰,所述的修饰有可能采取例如取代、添加、倒位或插入一个或多个核苷酸残基的形式。天然遗传环境理解为意指原初植物中的天然基因组位点或染色体位点或存在于基因组文库中。在基因组文库的情况下,优选地保留,至少部分地保留核酸序列的天然遗传环境。该环境分布在所述核酸序列的至少一侧并且具有至少50bp、优选至少500bp、特别优选至少1000bp、最优选至少5000bp序列长度。当通过非天然、合成性(“人工”)方法(例如诱变处理)修饰天然存在表达盒时,该表达盒-例如所述核酸序列的天然启动子与编码如上文所定义用于本发明方法中的多肽的相应核酸序列的天然存在组合-变成转基因表达盒。合适的方法例如在US 5,565,350或WO 00/15815中描述。
为本发明目的,如上所述,将转基因植物因此理解为意指本发明方法中所用诸核酸不处于它们在所述植物基因组中的天然基因座处,从而有可能同源或异源地表达所述核酸。然而,如所提及,转基因还意指尽管本发明的或本发明方法中所用的诸核酸处于它们在植物基因组中的天然位置处,然而相对于天然序列,它们的序列已经被修饰,和/或所述天然序列的调节序列已经被修饰。转基因优选地理解为意指本发明核酸在基因组中的非天然基因座处表达,即所述核酸的同源表达或优选异源表达发生。优选的转基因植物在本文中提及。
转化
如本文中提及的术语“导入”或“转化”包括转移外源多核苷酸至宿主细胞中,无论转化所用的方法是什么。能够后续克隆性增殖(无论通过器官发生或胚发生)的植物组织可以用本发明的基因构建体转化并且可完整植物以从中再生。所选的具体组织根据可用于并且最好适于正在进行转化的具体物种的克隆性增殖系统变化。示例性靶组织包括叶盘、花粉、胚、子叶、下胚轴、大配子体、愈伤组织、现存的分生组织(例如顶端分生组织、腋芽和根分生组织)和诱导的分生组织(例如子叶分生组织和下胚轴分生组织)。多核苷酸可以瞬时或稳定地导入宿主细胞并且可以非整合地维持,例如作为质粒。备选地,它可以整合至宿主基因组中。所得的转化植物细胞随后可以用来以本领域技术人员已知的方式再生出转化植物。
外来基因转移至植物基因组的过程称作转化。植物物种的转化现在是相当常规的技术。有利地,可以使用几种转化方法中的任何一种方法将目的基因导入合适的祖先细胞。描述用于转化并从植物组织或植物细胞再生出植物的方法可以用于瞬时转化或稳定转化。转化方法包括使用脂质体、电穿孔法、增加游离DNA摄入的化学品、DNA直接注射至植物、粒子枪轰击法、使用病毒或花粉的转化法和微量投射法(microprojection)。转化方法可以选自用于原生质体的钙/聚乙二醇法(Krens,F.A.等,(1982)Nature 296,72-74;Negrutiu I等,(1987)PlantMol Biol 8:363-373);原生质体的电穿孔法(Shillito R.D.等,(1985)Bio/Technol 3,1099-1102);对植物材料的微量注射法(Crossway A等,(1986)Mol.Gen Genet 202:179-185);DNA或RNA包被粒子轰击法(Klein TM等,(1987)Nature 327:70)、用(非整合性)病毒感染等。包括转基因作物植物在内的转基因植物优选通过农杆菌介导的转化法产生。有利的转化方法是植物原位(in planta)转化法。为此目的,例如有可能使农杆菌作用于植物种子或有可能用农杆菌接种植物分生组织。根据本发明,已经证明将转化的农杆菌悬液作用于完整植物或至少作用于花原基是特别有利的。随后培育该植物直至获得已处理植物的种子(Clough和Bent,Plant J.(1998)16,735-743)。用于农杆菌介导稻转化的方法包括用于稻转化的熟知方法,如在以下任意文献中描述的那些方法:欧洲专利申请EP 1198985A1,Aldemita和Hodges(Planta199:612-617,1996);Chan等,(Plant Mol Biol 22(3):491-506,1993),Hiei等,(Plant J 6(2):271-282,1994),其公开内容如充分所述那样通过引用的方式并入本文。在玉米转化的情况下,优选的方法如Ishida等,(Nat.Biotechnol 14(6):745-50,1996)或Frame等,(Plant Physiol129(1):13-22,2002)描述,其公开内容如充分所述那样通过引用的方式并入本文。所述方法还例如由B.Jenes等,Techniques for GeneTransfer,在:Transgenic Plants,第1卷,Engineering and Utilization,编者S.D.Kung和R.Wu,Academic Press(1993)128-143及在PotrykusAnnu.Rev.Plant Physiol.Plant Molec.Biol.42(1991)205-225)中描述。待表达的核酸或构建体优选地克隆至适于转化根瘤农杆菌(Agrobacterium tumefaciens)的载体,例如pBin19(Bevan等,Nucl.Acids Res.12(1984)8711)。被这种载体转化的农杆菌随后可以按照已知方式用于转化植物,例如作为模型使用的植物如拟南芥属植物(拟南芥在本发明范围不视为作物植物),或作物植物,例如烟草植物,所述方式例如是通过在农杆菌溶液中浸泡擦伤的叶或切碎的叶并随后在合适培养基中培育它们。借助根瘤农杆菌转化植物例如由和Willmitzer在Nucl.Acid Res.(1988)16,9877中描述或尤其从F.F.White,用于高等植物中基因转移的载体(Vectors for Gene Transfer inHigher Plants);在Transgenic Plants,第1卷,Engineering andUtilization,S.D.Kung和R.Wu编,Academic Press,1993,第15-38页中获知。
除了转化随后必需再生成完整植物的体细胞之外,也可以转化植物分生组织的细胞,并且尤其那些发育成配子的细胞。在这种情况下,转化的配子遵循天然的植物发育过程,从而产生转基因植物。因此,例如用农杆菌处理拟南芥属植物的种子并且从正在发育的植物获得种子,其中一定比例的所述植物被转化并且因此是转基因的[Feldman,KA和Marks MD(1987)Mol Gen Genet 208:274-289;FeldmannK(1992),在:编者C Koncz,N-H Chua和J Shell,Methods inArabidopsis Research.Word Scientific,Singapore,第274-289页]。备选方法基于反复移除花序并将莲座丛中心内的切除部位与转化的农杆菌温育,因而同样可以在较晚的时间点获得转化的种子(Chang(1994)Plant J.5:551-558;Katavic(1994)Mol Gen Genet,245:363-370)。然而,特别有效的方法是改良真空渗入法,如“浸花”法。在拟南芥属植物真空浸润法的情况下,用农杆菌悬液处理在降低压力下的完整植物[Bechthold,N(1993).C R Acad Sci Paris Life Sci,316:1194-1199],而在“浸花法”的情况下,将正在发育的花组织与表面活性剂处理过的农杆菌悬液短暂温育[Clough,SJ和Bent,AF(1998)The Plant J.16,735-743]。在这两种情况下均收获某个比例的转基因种子,并且这些种子可以通过在如上所述的选择条件下培育与非转基因种子区分。此外,质体的稳定转化是有利的,因为质体在大部分作物中以母系方式遗传,这降低或消除了借助花粉的转基因流动风险。叶绿体基因组的转化一般通过已经在Klaus等,2004[Nature Biotechnology 22(2),225-229]中示意性展示的方法实现。简而言之,将待转化的序列连同选择性标记基因一起克隆至同源于叶绿体基因组的侧翼序列之间。这些同源侧翼序列指导向原质体的位点特异性整合。已经对许多不同的植物物种描述质体转化过程并且在Bock(2001)基础研究和植物生物技术中的转基因质体(Transgenic plastids in basic research and plant biotechnology).J Mol Biol.2001年月21日;312(3):425-38或Maliga,P(2003)质体转化技术商业化进展(Progress towards commercialization of plastidtransformation technology),Trends Biotechnol.21,20-28中给出综述。其他的生物技术进展最近已经以无标记质体转化体的形式报道,其中可以通过瞬时共整合的标记基因产生所述无标记质体转化体(Klaus等,2004,Nature Biotechnology 22(2),225-229)。
T-DNA活化标签技术(T-DNA activation tagging)
T-DNA活化标签技术(Hayashi等,Science(1992)1350-1353)涉及以如此方式在目的基因的基因组区域内或基因编码区的上游或下游10kb处插入通常含有启动子(也可以是翻译增强子或内含子)的T-DNA,从而该启动子指导目标基因的表达。一般,目标基因的天然启动子对该基因表达的调节作用被破坏,并且该基因受新导入的启动子控制。该启动子一般嵌入T-DNA中。这种T-DNA随机地插入植物基因组,例如借助农杆菌感染,并且引起所插入T-DNA附近的基因调节的表达。所得的转基因植物表现显性表型,原因在于所导入启动子附近的基因受修饰的表达。
TILLING
术语“TILLING”是“基因组中定向诱导局部损伤法”的缩写并且指用于产生和/或鉴定核酸的诱变技术,其中所述核酸编码具有改良表达和/或活性的蛋白质。TILLING还允许选择携带此类突变变体的植物。这些突变变体可以表现在强度或在位置或在时间方面改良的表达(例如,如果所述突变影响启动子)。这些突变变体可以显示比其天然形式基因所表现活性更高的活性。TILLING联合了高密度诱变法与高通量筛选法。TILLING中一般所遵循的步骤是:(a)EMS诱变(Redei GP和Koncz C(1992)在Methods in Arabidopsis Research,Koncz C,Chua NH,Schell J,Singapore编,World Scientific Publishing Co,第16-82页;Feldmann等,(1994)在Meyerowitz EM,Somerville CR编,Arabidopsis.Cold Spring Harbor Laboratory Press,Cold Spring Harbor,NY,第137-172页;Lightner和Caspar T(1998)在J Martinez-Zapater,JSalinas编者,Methods on Molecular Biology第82卷.Humana Press,Totowa,NJ,第91-104页);(b)制备和汇集个体DNA;(c)PCR扩增目的区域;(d)变性和复性以导致异双链体形成;(e)DHPLC,其中汇集物中异双链体的存在被检测为色谱图中的一个额外峰;(f)鉴定突变个体;和(g)将突变PCR产物测序。用于TILLING的方法是本领域熟知的(McCallum等,(2000)Nat Biotechnol 18:455-457;综述参见Stemple(2004)Nat Rev Genet 5(2):145-50)。
同源重组
同源重组允许在基因组中限定的所选位置处导入所选核酸。同源重组是生物科学中常规用于低等生物如酵母或小立碗藓属(Physcomitrella)苔藓的标准技术。已经对模式植物(Offringa等,(1990)EMBO J 9(10):3077-84)和作物植物例如稻(Terada等,(2002)NatBiotech 20(10):1030-4;Iida和Terada(2004)Curr Opin Biotech 15(2):132-8)描述了用于植物中开展同源重组的方法,并且存在与靶生物无关而通常适用的方法(Miller等,Nature Biotechnol.25,778-785,2007)。
产量
术语“产量”通常意指经济价值的可测量结果,一般与指定作物、与面积并且与时间间隔有关。单个植物部分基于它们的数目、大小和/或重量而直接有助于产量,或实际产量是相对于某作物和年份的每平方米产量,这通过总产量(包括收获的和评估的产量)除以播种的平方米数确定。术语植物的“产量”可以涉及该植物的营养生物量(根和/或枝条生物量)、涉及繁殖器官和/或涉及繁殖体(如种子)。
早期生长势
“早期生长势”指活跃、健康、充分平衡的生长,尤其是植物生长早期期间,并且可以因提高的植物适应性所致,其中所述提高的植物适的原因是例如该植物更好地适应环境(即优化能量源的用途和在枝条与根之间的分配)。具有早期生长势的植物也显示提高的籽苗存活和更佳的作物建立,这往往产生高度均一的田块(作物以均一方式生长,即大多数植物在基本上相同的时间达到各个发育期)和往往形成更好且更高的产量。因而,早期生长势可以通过测量多种因素如千粒重(ThousandKernel Weight)、萌发百分数、出苗百分数、籽苗生长、籽苗高度、根长度、根和枝条生物量和许多其他因素等确定。
提高/改善/增强
术语“提高”、“改善”或“增强”是相互可交换的并且在应用含义上应当意指与如本文中定义的对照植物相比较,在TCP1、TCP2、底物蛋白-样,或SHR编码核酸或TCP1、TCP2、底物蛋白-样,或SHR多肽情况下,至少3%、4%、5%、6%、7%、8%、9%或10%、优选至少15%或20%、更优选地25%、30%、35%或40%更多的产量和/或生长,且与如本文中定义的对照植物相比较,在IPPT编码核酸或IPPT多肽情况下,至少5%、6%、7%、8%、9%、或10%、优选至少15%或20%、更优选地25%、30%、35%或40%更多的产量和/或生长。
种子产量
提高的种子产量自身可以表现为以下一个或多个指标:a)种子生物量(种子总重量)增加,这可以基于单粒种子基础和/或每株植物和/或每平方米;b)提高的每穗和/或每株植物花数目;c)提高的(饱满)种子数;d)提高的种子饱满率(其表述为饱满种子数与种子总数之间的比率);e)提高的收获指数,其表述为可收获部分(如种子)产量与总生物量的比率;f)提高的千粒重(TKW);(g)提高的初生穗数,这从计数的饱满种子数及它们的总重量外推出来。提高的TKW可以因增加的种子尺寸和/或种子重量引起,并且也可以因胚尺寸和/或胚乳尺寸增加引起。
种子产量的提高也可以表现为种子尺寸和/或种子体积的增加。此外,种子产量提高也可以本身表现为种子面积和/或种子长度和/或种子宽度和/或种子周长的提高。提高的产量也可以产生改良的构造,或可以因改良的构造而出现。
绿度指数
从植物的数字图像计算如本文中所用的“绿度指数”。对属于图像上植物目标的每个像素计算绿色值与红色值的比率(在编码颜色的RGB模式中)。绿度指数表述为绿色/红色比超过给定阈值的像素百分数。在正常生长条件下,在盐胁迫生长条件下和在养分有效性降低的生长条件下,植物的绿度指数在开花前的最后成像中测量。相反,在干旱胁迫生长条件下,植物的绿度指数在干旱后的首次成像中测量。
植物
本文中所用的术语“植物”包括完整植物、植物的祖先及子代和包括种子、枝条、茎、叶、根(包括块茎)、花和组织、器官在内的植物部分,其中每种前述对象包含目的基因/核酸。术语“植物”也包括植物细胞、悬浮培养物、愈伤组织、胚、分生组织区、配子体、孢子体、花粉和小孢子,同样其中每种前述对象包含目的基因/核酸。
在本发明方法中特别有用的植物包括属于植物界(Viridiplantae)超家族、尤其单子叶和双子叶植物的全部植物,包括饲用或饲料豆科植物、观赏植物、粮食作物、树或灌木,其中所述植物选自包含以下物种的名单:槭树属物种(Acer spp.)、猕猴桃属物种(Actinidia spp.)、秋葵属物种(Abelmoschus spp.)、剑麻(Agave sisalana)、冰草属物种(Agropyron spp.)、匍匐剪股颖(Agrostis stolonifera)、葱属物种(Alliumspp.)、苋属物种(Amaranthus spp.)、欧洲海滨草(Ammophila arenaria)、凤梨(Ananas comosus)、番荔枝属物种(Annona spp.)、旱芹(Apiumgraveolens)、蜘蛛兰属物种(Arachis spp.)、木波罗属物种(Artocarpusspp.)、石刁柏(Asparagus officinalis)、燕麦属物种(Avena spp.)(例如燕麦(Avena sativa)、野燕麦(Avena fatua)、比赞燕麦(Avena byzantina)、野燕麦原变种(Avena fatua var.sativa)、杂种燕麦(Avena hybrida)、阳桃(Averrhoa carambola)、箣竹属物种(Bambusa sp.)、冬瓜(Benincasahispida)、巴西栗(Bertholletia excelsea)、甜菜(Beta vulgaris)、芸苔属物种(Brassica spp.)(例如欧洲油菜(Brassica napus)、芜青物种(Brassicarapa ssp.)[卡诺拉油菜、油菜籽油菜(oilseed rape)、蔓青(turnip rape)])、Cadaba farinosa、茶(Camellia sinensis)、美人蕉(Canna indica)、大麻(Cannabis sativa)、辣椒属物种(Capsicum spp.)、Carex elata、番木瓜(Carica papaya)、大果假虎刺(Carissa macrocarpa)、山核桃属物种(Carya spp.)、红花(Carthamus tinctorius)、栗属物种(Castanea spp.)、美洲木棉(Ceiba pentandra)、苦苣(Cichorium endivia)、樟属物种(Cinnamomum spp.)、西瓜(Citrullus lanatus)、柑桔属物种(Citrusspp.)、椰子属物种(Cocos spp.)、咖啡属物种(Coffea spp.)、芋头(Colocasia esculenta)、非洲梧桐属物种(Cola spp.)、黄麻属物种(Corchorus sp.)、芫荽(Coriandrum sativum)、榛属物种(Corylus spp.)、山楂属物种(Crataegus spp.)、番红花(Crocus sativus)、南瓜属物种(Cucurbita spp.)、香瓜属物种(Cucumis spp.)、菜蓟属物种(Cynaraspp.)、胡萝卜(Daucus carota)、山马蝗属物种(Desmodium spp.)、龙眼(Dimocarpus longan)、薯蓣属物种(Dioscorea spp.)、柿树属物种(Diospyros spp.)、稗属物种(Echinochloa spp.)、油棕属(Elaeis)(例如油棕(Elaeis guineensis)、美洲油棕(Elaeis oleifera)、穇子(Eleusinecoracana)、蔗茅属物种(Erianthus sp.)、枇杷(Eriobotrya japonica)、桉属物种(Eucalyptus sp.)、红仔果(Eugenia uniflora)、荞麦属物种(Fagopyrum spp.)、水青冈属物种(Fagus spp.)、苇状羊茅(Festucaarundinacea)、无花果(Ficus carica)、金桔属物种(Fortunella spp.)、草莓属物种(Fragaria spp.)、银杏(Ginkgo biloba)、大豆属物种(Glycinespp.)(例如大豆(Glycine max)、大豆(Soja hispida)或大豆(Soja max)、陆地棉(Gossypium hirstum)、向日葵属物种(Helianthus spp.)(例如向日葵(Helianthus annuus)、长管萱草(Hemerocallis fulva)、木槿属物种(Hibiscus spp.)、大麦属物种(Hordeum spp.)(例如大麦(Hordeumvulgare)、甘薯(Ipomoea batatas)、核桃属物种(Juglans spp.)、莴苣(Lactuca sativa)、山黧豆属物种(Lathyrus spp.)、兵豆(Lens culinaris)、亚麻(Linum usitatissimum)、荔枝(Litchi chinensis)、百脉根属物种(Lotus spp.)、棱角丝瓜(Luffa acutangula)、羽扇豆属物种(Lupinusspp.)、Luzula sylvatica、番茄属物种(Lycopersicon spp.)(例如番茄(Lycopersicon esculentum、Lycopersicon lycopersicum、Lycopersiconpyriforme)、硬皮豆属物种(Macrotyloma spp.)、苹果属物种(Malusspp.)、凹缘金虎尾(Malpighia emarginata)、牛油果(Mammeaamericana)、芒果(Mangifera indica)、木薯属物种(Manihot spp.)、人心果(Manilkara zapota)、苜蓿(Medicago sativa)、草木樨属物种(Melilotus spp.)、薄荷属物种(Mentha spp.)、芒(Miscanthus sinensis)、苦瓜属物种(Momordica spp.)、黑桑(Morus nigra)、芭蕉属物种(Musaspp.)、烟草属物种(Nicotiana spp.)、木犀榄属物种(Olea spp.)、仙人掌属物种(Opuntia spp.)、鸟足豆属物种(Ornithopus spp.)、稻属些物种(Oryza spp.)(例如稻(Oryza sativa)、阔叶稻(Oryza latifolia)、稷(Panicum miliaceum)、柳枝稷(Panicum virgatum)、鸡蛋果(Passifloraedulis)、欧防风(Pastinaca sativa)、狼尾草属物种(Pennisetum sp.)、鳄梨属物种(Persea spp.)、欧芹(Petroselinum crispum)、虉草(Phalarisarundinacea)、菜豆属物种(Phaseolus spp.)、猫尾草(Phleum pratense)、刺葵属物种(Phoenix spp.)、南方芦苇(Phragmites australis)、酸浆属物种(Physalis spp.)、松属物种(Pinus spp.)、阿月浑子(Pistacia vera)、豌豆属物种(Pisum spp.)、早熟禾属物种(Poa spp.)、杨属物种(Populusspp.)、牧豆草属物种(Prosopis spp.)、李属物种(Prunus spp.)、番石榴属物种(Psidium spp.)、石榴(Punica granatum)、西洋梨(Pyruscommunis)、栎属物种(Quercus spp.)、萝卜(Raphanus sativus)、波叶大黄(Rheum rhabarbarum)、茶藨子属物种(Ribes spp.)、蓖麻(Ricinuscommunis)、悬钩子属物种(Rubus spp.)、甘蔗属物种(Saccharum spp.)、柳属物种(Salix sp.)、接骨木属物种(Sambucus spp.)、黑麦(Secalecereale)、胡麻属物种(Sesamum spp.)、白芥属物种(Sinapis sp.)、茄属物种(Solanum spp.)(例如马铃薯(Solanum tuberosum)、红茄(Solanumintegrifolium)或番茄(Solanum tuberosum)、两色蜀黍(Sorghumbicolor)、菠菜属物种(Spinacia spp.)、蒲桃属物种(Syzygium spp.)、万寿菊属物种(Tagetes spp.)、酸豆(Tamarindus indica)、可可树(Theobroma cacao)、车轴草属物种(Trifolium spp.)、小黑麦属物种(Triticale sp.)、Triticosecale rimpaui、小麦属物种(Triticum spp.)(例如普通小麦(Triticum aestivum)、硬粒小麦(Triticum durum)、圆柱小麦(Triticum turgidum)、Triticum hybernum、马卡小麦(Triticum macha)、普通小麦(Triticum sativum)或普通小麦(Triticum vulgare)、小金莲花(Tropaeolum minus)、金莲花(Tropaeolum majus)、越桔属物种(Vaccinium spp.)、野碗豆属物种(Vicia spp.)、豇豆属物种(Vigna spp.)、香堇(Viola odorata)、葡萄属物种(Vitis spp.)、玉蜀黍(Zea mays)、Zizania palustris、枣属物种(Ziziphus spp.)及其他。
发明详述
令人惊讶地,现已发现:调节植物中编码TCP1或TCP2多肽或底物蛋白-样多肽的核酸表达产生相对于对照植物具有增强的产量相关性状的植物。根据第一实施方案,本发明提供了用于相对于对照植物增强植物中产量相关性状的方法,其包括调节植物中编码TCP1或TCP2多肽或底物蛋白-样多肽的核酸表达。
用于调节(优选提高)编码TCP1或TCP2多肽的核酸表达的优选方法是在植物中引入并表达编码TCP1或TCP2多肽或底物蛋白-样多肽的核酸。
此外,令人惊讶地,现已发现:增加植物种子中编码如本文定义的IPPT多肽的核酸序列表达产生相对于对照植物具有增强的产量相关性状的植物。根据第一实施方案,本发明提供了用于相对于对照植物增强植物中产量相关性状的方法,其包括增加植物种子中编码IPPT多肽的核酸序列的表达。
用于增加植物种子中编码IPPT多肽的核酸序列表达的优选方法是在植物种子中引入并表达编码IPPT多肽的核酸序列。
还令人惊讶地,现已发现:调节在亚最佳养分可利用性条件下培育的植物中编码SHR多肽的核酸表达产生相对于对照植物具有增强的产量相关性状的植物。还令人惊讶地,现已发现:调节在无养分限制条件下培育的植物中编码SHR多肽的核酸表达产生相对于对照植物具有提高的千粒重(TKW)的植物。
根据一实施方案,本发明提供了用于相对于对照植物增强产量相关性状的方法,其包括调节在亚最佳养分可利用性条件下培育的植物中编码SHR多肽的核酸的表达。
本发明另一实施方案提供了用于相对于对照植物提高植物千粒重(TKW)的方法,其包括调节在无养分限制条件下培育的植物中编码SHR多肽的核酸的表达。
用于调节(优选提高)编码SHR多肽的核酸表达的优选方法是在植物中引入并表达编码SHR多肽的核酸。
对于TCP1或TCP2多肽/基因,下文任何对“本发明方法中有用的蛋白质”的谈及意指如本文中定义的TCP1或TCP2多肽。下文任何对“用于本发明方法中的核酸”的谈及指能够编码这种TCP1或TCP2多肽的核酸。待导入植物(并且因而在实施本发明方法中有用)的核酸是编码现在将描述的蛋白质类型的任意核酸,下文也称作“TCP1核酸”或“TCP1基因”或“TCP2核酸”或“TCP2基因”。
对于底物蛋白-样多肽/基因,下文任何对“本发明方法中有用的蛋白质”的谈及意指如本文中定义的底物蛋白-样多肽。下文任何对“用于本发明方法中的核酸”的谈及指能够编码这种底物蛋白-样多肽的核酸。待导入植物(并且因而在实施本发明方法中有用)的核酸是编码现在将描述的蛋白质类型的任意核酸,下文也称作“底物蛋白-样核酸”或“底物蛋白-样基因”。
对于IPPT多肽/基因,下文任何对“本发明方法中有用的蛋白质”的谈及意指如本文中定义的IPPT多肽。下文任何对“用于本发明方法中的核酸序列”的谈及指能够编码这种IPPT多肽的核酸序列。待导入植物(并且因而在实施本发明方法中有用)的核酸序列是编码现在将描述的多肽类型的任意核酸序列,下文也称作“IPPT核酸序列”或“IPPT基因”。
对于SHR多肽/基因,下文任何对“本发明方法中有用的蛋白质”的谈及意指如本文中定义的SHR多肽。下文任何对“用于本发明方法中的核酸”的谈及指能够编码这种SHR多肽的核酸。待导入植物(并且因而在实施本发明方法中有用)的核酸是编码现在将描述的蛋白质类型的任意核酸,下文也称作“SHR核酸”或“SHR基因”。
如本文中定义的“TCP1多肽”指这样的任意多肽,其包含:
(i)与图1所示任一序列的TCP结构域以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的TCP结构域;和
(ii)与图1所示任一序列的结构域A以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iii)与图1所示任一序列的结构域B以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iv)与图1所示任一序列的结构域C以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域。
根据优选的实施方案,TCP1多肽包含:
(i)与图1中Ms_TCP_sugar所示的序列的TCP结构域以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的TCP结构域;和
(ii)与图1中Ms_TCP_sugar所示的序列的结构域A以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iii)与图1中Ms_TCP_sugar所示的序列的结构域B以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iv)与图1中Ms_TCP_sugar所示的序列的结构域C以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域。
如本文中定义的“TCP2多肽”指这样的任意多肽,其包含:
(i)与图2所示任意序列的TCP结构域以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的TCP结构域;和
(ii)与图2所示任意序列的结构域1以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iii)与图2所示任意序列的结构域2以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iv)与图2所示任意序列的结构域3以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域。
根据优选的实施方案,TCP2多肽包含:
(i)与图2中Mt_TCP2_sugar所示的序列的TCP结构域以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的TCP结构域;和
(ii)与图2中Mt_TCP2_sugar所示的序列的结构域1以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iii)与图2中Mt_TCP2_sugar所示的序列的结构域2以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iv)与图2中Mt_TCP2_sugar所示的序列的结构域3以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域。
此外,TCP2多肽可包含以下之一或全部:
(v)与图2所示任意序列的结构域4以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;
(vi)与图2所示任意序列的结构域5以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域。
优选地,结构域4与图2中Mt_TCP2_sugar所示的序列的结构域4以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性。
优选地,结构域5与图2中Mt_TCP2_sugar所示的序列的结构域5以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性。
TCP1或TCP2蛋白质以增加的优选顺序分别与SEQ ID NO:2或SEQ ID NO:4所示的氨基酸具有至少25%、26%、27%、28%、29%、30%、31%、32%、33%、34%、35%、36%、37%、38%、39%、40%、41%、42%、43%、44%、45%、46%、47%、48%、49%、50%、51%、52%、53%、54%、55%、56%、57%、58%、59%、60%、61%、62%、63%、64%、65%、66%、67%、68%、69%、70%、71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或99%全局序列同一性。使用总体比对算法如程序GAP(GCG Wisconsin Package,Accelrys)中的Needleman Wunsch算法,优选地采用默认参数,确定全局序列同一性。与全局序列同一性相比较,仅考虑保守的结构域或基序时,该序列同一性通常会更高。
优选地,TCP1或TCP2多肽序列在构建诸如图2所示的TCP系统树时,与包含SEQ ID NO:2或SEQ ID NO:4所示的氨基酸序列的TCP1或TCP2多肽组聚簇,而不与任何其他组聚簇。
如本文所定义,“底物蛋白-样多肽”指在其N端二分之一包含ENTH结构域(SMART检索号SM00273)的任意多肽。ENTH结构域为本领域已知和描述于InterPro数据库:ENTH(底物蛋白N端同源)结构域长度约150个氨基酸,总是发现其定位于蛋白质的N端。该结构域形成紧密的球状结构,由通过不同长度的环连接的9个α-螺旋组成。一般拓扑学由以右手旋转连续堆叠的三个螺旋发夹决定。N端螺旋折回形成深的碱性沟,其形成Ins(1,4,5)P3配体的结合袋。配体由来自周围的α-螺旋的残基配位,且所有三个磷酸都多价配位。Ins(1,4,5)P3的配位表明ENTH是对特定头部基团特异的。已发现包含此结构域的蛋白质结合PtdIns(4,5)P2和PtdIns(1,4,5)P3,这表明此结构域是膜相互作用组件。包含此结构域的蛋白质的主要功能似乎是在胞吞作用中作为辅助网格蛋白衔接头,底物蛋白能在脂单层上募集和促进网格蛋白多聚化,但其可以在信号发放和肌动蛋白调节中具有其他的作用。底物蛋白引起具有高PtdIns(4,5)P2含量的膜的强度膜弯曲和成管、甚至片段化。结合膜的底物蛋白通过将N端螺旋插入双层结构的外层,推开头部基团来促进膜变形。这将降低膜弯曲为小泡所需的能量,使网格蛋白笼更易于固定和稳定弯曲的膜。这表明底物蛋白在小泡出芽中的先导作用,因为其提供了膜内陷和网格蛋白多聚化间的驱动力和联系(注释IPR013809)。
优选地,用于本发明方法中的底物蛋白-样多肽还包含两个和多个以下基序:
基序1:(V/I)(L/R)(D/E)AT(S/D/N)(N/D/E/S)E(P/S)WGPHG(T/S/E)(SEQ ID NO:48)
优选地,基序1是:(V/I)LDAT(S/D/N)(N/D)E(P/S)WGPHG(T/S)
更优选地,基序1是VLDATDNEPWGPHGT
基序2:
F(Q/E)(Y/F)(I/L/V/R/K)(D/E)(S/P/A)(S/G/N/Q/R)G(R/K)D(Q/V/A/H/E)G(S/N/L/I/V)NVR(SEQ ID NO:49)
优选地,基序2是:
F(Q/E)(Y/F)(I/L/V)(D/E)(S/P)(S/G/N)G(R/K)D(Q/V/A)G(S/N/L/I)NVR
更优选地,基序2是FEYVEPNGKDVGINVR
基序3:
(E/S/A/Q)(V/I/E/A)R(Q/E/D/N)KA(A/L/V/E)(A/V/S/R/K)(N/T)(R/A)(D/E/N/G)K(SEQ ID NO:50)
优选地,基序3是:
(E/S/A)(V/I)R(Q/E/D/N)KA(A/L/V)(A/V/S)(N/T)R(D/E/N)K
更优选地,基序3是EIRDKAVANRNK
基序4:WAD(T/S)LSRGL(V/I)(SEQ ID NO:51)
优选地,基序4是:WADSLSRGLI
基序5:L(A/S)D(I/V)G(I/V)(D/V)(F/G)(D/E/P/G)(SEQ ID NO:52)
优选地,基序5是:LADVGVVGD
除了上述基序,用于本发明方法中的蛋白质还优选包含以其天然形式的一个或多个下述基序:
基序6(a至c):以下四肽之一:GGYG、GSYG或GGYD(SEQ ID NO:53、54、55)
基序7(a至d):以下四肽之一:SAAS、SSAS、SSAP或SSAT(SEQ IDNO:56、57、58、59)
基序8(a至e):以下四肽之一:DEFD、DFFD、DDDF、EDDF、或DDFD(SEQ ID NO:60、61、62、63、64)
备选地,底物蛋白-样蛋白的同源物以增加的优选顺序与SEQ IDNO:44所示的氨基酸具有至少25%、26%、27%、28%、29%、30%、31%、32%、33%、34%、35%、36%、37%、38%、39%、40%、41%、42%、43%、44%、45%、46%、47%、48%、49%、50%、51%、52%、53%、54%、55%、56%、57%、58%、59%、60%、61%、62%、63%、64%、65%、66%、67%、68%、69%、70%、71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或99%全局序列同一性,条件是同源物蛋白包含两个或多个上面概括的保守结构域。使用总体比对算法如程序GAP(GCGWisconsin Package,Accelrys)中的Needleman Wunsch算法,优选地采用默认参数,确定全局序列同一性。与全局序列同一性相比较,仅考虑保守的结构域或基序时,该序列同一性通常会更高。例如,当ENTH结构域在底物蛋白-样多肽中比较时,与全局序列同一性相比较,该序列同一性通常会更高。
优选地,所述多肽序列当其用于构建诸如图3(Holstein和Oliviusson,Protoplasma 226,13-21,2005)所示的系统树时,与包含SEQID NO:44所示的氨基酸序列的底物蛋白-样多肽组聚簇,而不与任何其他组聚簇。
如本文中定义的“IPPT多肽”指这样的任意多肽序列,其包含:(i)InterPro登录号IPR002627的tRNA异戊烯基转移酶结构域;和(ii)N端ATP/GTP结合位点基序A(P-环)。
备选地或额外地,如本文中定义的“IPPT多肽”指这样的任意多肽,其(i)以增加的优选顺序与SEQ ID NO:199所示的N端ATP/GTP结合位点基序A(P环)具有至少70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性;和以增加的优选顺序与以下一个或多个基序具有至少70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性:(ii)SEQ ID NO:200所示的保守基序ⅠDSR(Q/L)(V/L/I);或(iii)SEQ ID NO:201所示的保守基序Ⅱ(N/D/S/T)(I/V)GTAKP(T/S);或(ii)SEQ ID NO:202所示的保守基序Ⅲ L(V/A/I)GG(S/T)GLY;或(iv)SEQ ID NO:203所示的保守基序ⅣF/Y/L)AK(R/K/Q)Q(R/K/M)TWFR。
备选地或额外地,如本文中定义的“IPPT多肽”指这样的任意多肽,其以增加的优选顺序与SEQ ID NO:144所示IPPT多肽或与本文表A4中给出的任意多肽序列具有至少35%、40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性。
备选地或额外地,“IPPT多肽”能互补缺乏内源IPPT活性的酵母mod5突变株,或能互补缺乏内源IPPT活性的大肠杆菌(E.coli)miaA突变株。
如本文中定义的“SHR多肽”这样的任意全长多肽,所述多肽在构建诸如图14所示的GRAS系统树时,与包含SEQ ID NO:209所示的氨基酸序列的SHR多肽组聚簇,而不与任何其他组聚簇。
作为植物转录因子GRAS家族的成员,SHR多肽可以包含GRAS基因家族的典型特征。此类典型特征包含高度保守的C端区域,但可变的N段区域。高度保守的C端区域包含通常见于以下顺序的五个不同基序:
1.亮氨酸七残基重复序列(LHR1);
2.VHIID基序;
3.亮氨酸七残基重复序列Ⅱ(LHRII);
4.PFYRE基序;和
5.SAW基序。
LHR I似乎由被间隔区隔断的两个重复单位组成,该间隔区常包含已知其破坏α-螺旋结构的脯氨酸残基。LHR I内的这两个单位彼此不同相。LHR IA与见于其他蛋白质的LHR类似,由3-5个规则的七残基重复序列组成。LHR IB较短,通常仅由2个此类重复。在LHR II中,可以在几乎所有GRAS家族成员的此区域内鉴定特定的亮氨酸七残基重复序列,重复数少,通常为两个或三个。
虽然其并非绝对保守,但已于在该家族的所有成员中识别VHIID序列:1位、3位和4位缬氨酸、异亮氨酸和亮氨酸的替换产生许多变换。在我们称之为VHIID基序的更大区域内,P-N-H-D-Q-L残基绝对保守。脯氨酸和天冬酰胺残基间的间隔在所有成员中是一致的,组氨酸、天冬氨酸、谷氨酰胺和亮氨酸残基间的间隔也如此。VHIID基序在其C端以为了简明而称之为LRITG的保守序列相邻。
大部分来自此共有序列的偏离代表保守性变化。
在PFYRE基序中,P是绝对保守的。在PFYRE结构域中,序列是基本线性对应的,此区域的一些部分在GRAS家族的所有成员中显示高度的序列相似性。
SAW基序的特征在于三对绝对保守的残基:R-E、W-G和W-W。见于几乎这些序列C端的W-W对显示间隔的绝对保守,W-G对也如此。
除了在GRAS系统树中与其他SHR多肽聚簇的SHR多肽,优选用于本发明方法中的SHR多肽的C端区以增加的优选顺序与SEQ IDNO:209所示的氨基酸的C端区具有至少50%、51%、52%、53%、54%、55%、56%、57%、58%、59%、60%、61%、62%、63%、64%、65%、66%、67%、68%、69%、70%、71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%、或99%的全局序列同一性。
使用总体比对算法如程序GAP(GCG Wisconsin Package,Accelrys)中的Needleman Wunsch算法,优选地采用默认参数,确定全局序列同一性。与全局序列同一性相比较,仅考虑保守的结构域或基序时,该序列同一性通常会更高。
术语“结构域”和“基序”在文中的“定义”部分定义。存在用于鉴定结构域的专业数据库,如SMART(Schultz等(1998)Proc.Natl.Acad.Sci.美国95,5857-5864;Letunic等(2002)Nucleic Acids Res 30,242-244)、InterPro(Mulder等,(2003)Nucl.Acids.Res.31,315-318)、Prosite(Bucher和Bairoch(1994),A generalized profile syntax forbiomolecular sequences motifs and its function in automatic sequenceinterpretation(用于生物分子序列基序的广义图谱语法及其在自动化序列判读中的功能),(在)ISMB-94;第二届分子生物学智能系统国际会议文集.Altman R.,Brutlag D.,Karp P.,Lathrop R.,Searls D.编,第53-61页,AAAI Press,Menlo Park;Hulo等,Nucl.Acids.Res.32:D134-D137,(2004))或Pfam(Bateman等,Nucleic Acids Research 30(1):276-280(2002))。用于计算机芯片上分析蛋白质序列的一组工具可在ExPASY蛋白质组学服务器上获得(瑞士生物信息学研究所(Gasteiger等,ExPASy:the proteomics server for in-depth protein knowledge andanalysis(用于深入认识和分析蛋白质的蛋白组学服务器),NucleicAcids Res.31:3784-3788(2003))。结构域或基序也可以使用常规技术如通过序列比对而鉴定。
在下面的本文实施例4中提供了多肽序列SEQ ID NO:144的分析。例如,SEQ ID NO:144所示的IPPT多肽包含具有InterPro登录号IPR002627的tRNA异戊烯基转移酶。还可使用常规技术识别结构域,如通过序列比对。图3显示了本文表A4的多肽的比对。此类比对对识别IPPT多肽间最保守的结构域或基序有用,如SEQ ID NO:200至203(包含在SEQ ID NO:144)所示的保守结构域。
用于比对序列以做比较的方法是本领域熟知的,此类方法包括GAP、BE STFIT、BLAST、FASTA和TFASTA。GAP使用Needleman和Wunsch算法((1970)J Mol Biol 48:443-453)以找到使匹配数最大化并使空位数最小化的两个序列的总体(即覆盖完整序列的)比对结果。BLAST算法(Altschul等,(1990)J Mol Biol 215:403-10)计算序列同一性百分数并开展两个序列之间相似性的统计分析。用于开展BLAST分析的软件是通过国家生物技术信息中心(NCBI)可公开获得的。同源物可以使用例如ClustalW多重序列比对算法(1.83版本),以默认配对比对参数和百分数评分方法轻易地鉴定。相似性和同一性的总体百分数也可以使用MatGAT软件包中的可用方法之一确定(Campanella等,BMC Bioinformatics,2003Jul 10;4:29.MatGAT:使用蛋白质序列或DNA序列产生相似性/同一性矩阵的一种应用(an application thatgenerates similarity/identity matrices using protein or DNAsequences))。如本领域技术人员会显而易见,可以进行少许手工编辑以优化保守基序之间的比对。此外,作为使用全长序列鉴定同源物的替代,也可以使用特定的结构域。利用上文提及的程序,使用默认参数,可以在完整核酸或氨基酸序列或所选的结构域保守基序范围内确定序列同一性值。对于局部比对,Smith-Waterman算法是特别有用的(SmithTF,Waterman MS(1981)J.Mol.Biol 147(1);195-7)。
另外,TCP1和TCP2多肽(至少以其天然形式)一般具有DNA结合活性。测量DNA结合活性的工具和技术是本领域公知的。实施例6中提供了进一步的详述。
此外,TCP1和TCP2多肽,当其根据如实施例章节所概述的本发明方法在稻中表达时,产生具有增强的产量相关性状,尤其具有提高的种子产量的植物。
另外,底物蛋白-样多肽(至少以其天然形式)一般具有脂质结合活性。测量脂质结合活性的工具和技术是本领域公知的。例如,Hom等.(J.Mol.Biol.373,412-423,2007)描述了通过ENTH结构域的脂质结合。实施例6中提供了进一步的详述。
此外,底物蛋白-样多肽,当其根据如实施例7和8所概述的本发明方法在稻中表达时,产生具有增强的产量相关性状,尤其具有提高的种子总产量、饱满率、种子总数量和饱满种子数中的一种或多种的植物。
本文实施例3的表B4中描述了SEQ ID NO:144所示的IPPT多肽和表A4中所列IPPT多肽间的同一性百分比,其可以低至39%氨基酸序列同一性。
蛋白质亚细胞定位预测的任务是重要的并且得到充分研究。知晓蛋白质的定位有助于阐明其功能。用于蛋白质定位的实验方法的范围从免疫定位至使用绿色荧光蛋白(GFP)或β-葡糖醛酸酶(GUS)对蛋白质加标签。虽然此类方法与计算性方法相比繁琐费累,然而它准确。最近在从序列数据计算性预测蛋白质定位方面取得长足进展。在瑞士生物信息研究所维护的ExPASy蛋白质组工具上可获得本领域技术人员熟知算法当中的例如PSort、TargetP、ChloroP、LocTree、Predotar、LipoP、MITOPROT、PATS、PTS1、SignalP、TMHMM等。
另外,用于本发明方法中的IPPT多肽(至少以它们的天然形式)一般能够将异戊烯基部分从δ(2)-二甲基丙烯基二磷酸(DMAPP)或羟甲基丁烯基二磷酸(HMBDP)转移到某些tRNA的第37位腺嘌呤上。存在许多可以测定此类酶活性的测定,包括具有缺陷型内源IPPT活性(由MOD5基因编码;Golovko等(2002)Plant Molec Biol 49:161-169)的酵母菌株的互补测定、具有缺陷型内源IPPT活性(由miaA基因编码;Dihanich等(1987)Mol Cell Biol 7:177-184)的大肠杆菌菌株的互补测定,或tRNA中细胞激动素的定量(Gray等(1996)Plant Physiol 110:431-438,Miyawaki等(2006)Proc Natl Acad SCi USA 103(44):16598-16603)。
此外,SHR多肽,当其在亚最佳养分可利用性条件下培育的稻中表达时,产生相对于对照植物具有增强的产量相关性状的植物。SHR多肽,当其在无养分限制条件下培育的稻中表达时,产生相对于对照植物具有提高的千粒重(TKW)的植物。
对于TCP1和TCP2,本发明通过用SEQ ID NO:1所示的核酸序列转化植物进行说明,其中所述核酸序列编码SEQ ID NO:2的多肽序列,和通过用SEQ ID NO:3所示的核酸序列转化植物进行说明,其中所述核酸序列编码SEQ ID NO:4的多肽序列。然而,本发明的实施不限于这些序列;本发明的方法可以有利地使用编码TCP1或编码TCP2的任意核酸或如本文中定义的TCP1或TCP2多肽实施。
对于TCP1和TCP2多肽,本文实施例1给出了编码TCP1和TCP2多肽的核酸的实例。此类核酸可用于实施本发明的方法。实施例1中给出的核酸序列编码的氨基酸序列是SEQ ID NO:2所示的TCP1多肽的直向同源物和旁系同源物的序列实例,实施例1中给出的核酸序列编码的氨基酸序列是SEQ ID NO:4所示的TCP2多肽的直向同源物和旁系同源物的序列实例,术语“直向同源物”和“旁系同源物”如文中所定义。其他直向同源物和旁系同源物可以通过开展所谓交互性blast搜索而容易地鉴定。这通常涉及第一BLAST,其中所述的第一BLAST包括提交查询序列(例如使用实施例1中列出的任意序列)用于针对任一序列数据库(如公众可用的NCBI数据库)的BLAST搜索。当从核苷酸序列开始时,一般使用BLASTN或TBLASTX(使用标准默认值),并且当从蛋白质序列开始时,可以使用BLASTP或TBLASTN(使用标准默认值)。任选地可以筛选BLAST结果。随后提交筛选结果和非筛选结果的全长序列以针对来自生物的序列进行反向BLAST(第二BLAST),其中查询序列来自所述的生物(其中在查询序列是SEQ IDNO:1至SEQ ID NO:4的情况下,第二BLAST因而将针对苜蓿序列)。随后比较第一和第二BLAST搜索的结果。若来自第一BLAST的高阶位命中源自与查询序列从其中衍生的物种相同的物种,反向BLAST随后理想地在最高命中的查询序列中产生,则鉴定到旁系同源物;若第一BLAST的中的高阶位命中不源自与查询序列从其中衍生的物种相同的物种,并且优选地在反向BLAST时产生属于最高命中之列的查询序列,则鉴定到直向同源物。
对于底物蛋白-样序列,本发明通过用SEQ ID NO:43所示的核酸序列转化植物进行说明,其中所述核酸序列编码SEQ ID NO:44的多肽序列。然而,本发明的实施不限于这些序列;本发明的方法可以有利地使用如本文中定义的任意底物蛋白-样编码核酸或底物蛋白-样多肽实施。
对于底物蛋白-样序列,编码底物蛋白-样多肽的核酸的实例在本文实施例1的表A3中给出。此类核酸可用于实施本发明的方法。实施例1的表A3中给出的氨基酸序列是SEQ ID NO:44所示的底物蛋白-样多肽的直向同源物和旁系同源物的序列实例,术语“直向同源物”和“旁系同源物”如文中所定义。其他直向同源物和旁系同源物可以通过开展所谓交互性blast搜索而容易地鉴定。这通常涉及第一BLAST,其中所述的第一BLAST包括提交查询序列(例如使用实施例1的表A3中列出的任一序列)用于针对任一序列数据库(如公众可用的NCBI数据库)的BLAST搜索。当从核苷酸序列开始时,一般使用BLASTN或TBLASTX(使用标准默认值),并且当从蛋白质序列开始时,可以使用BLASTP或TBLASTN(使用标准默认值)。任选地可以筛选BLAST结果。随后提交筛选结果和非筛选结果的全长序列以针对来自生物的序列进行反向BLAST(第二BLAST),其中查询序列来自所述的生物(其中在查询序列是SEQ ID NO:43或SEQ ID NO:44的情况下,第二BLAST因而将针对拟南芥序列)。随后比较第一和第二BLAST搜索的结果。若来自第一BLAST的高阶位命中源自与查询序列从其中衍生的物种相同的物种,反向BLAST随后理想地在最高命中的查询序列中产生,则鉴定到旁系同源物;若第一BLAST的中的高阶位命中不源自与查询序列从其中衍生的物种相同的物种,并且优选地在反向BLAST时产生属于最高命中之列的查询序列,则鉴定到直向同源物。
对于IPPT,本发明通过用SEQ ID NO:143所示的核酸序列转化植物进行说明,其中所述核酸序列编码SEQ ID NO:144的多肽序列。然而,本发明的实施不限于这些序列;本发明的方法可以有利地使用如本文中定义的任意编码IPPT多肽的核酸序列实施。
对于IPPT,编码IPPT多肽的核酸序列的实例在本文实施例1的表A4中给出。此类核酸序列可用于实施本发明的方法。实施例1的表A4中给出的核酸序列是SEQ ID NO:144所示的IPPT多肽的直向同源物和旁系同源物的序列实例,术语“直向同源物”和“旁系同源物”如文中所定义。其他直向同源物和旁系同源物可以通过开展所谓交互性blast搜索而容易地鉴定。这通常涉及第一BLAST,其中所述的第一BLAST包括提交查询序列(例如使用实施例1的表A4中列出的任一序列)用于针对任一序列数据库(如公众可用的NCBI数据库)的BLAST搜索。当从核苷酸序列开始时,一般使用BLASTN或TBLASTX(使用标准默认值),并且当从蛋白质序列开始时,可以使用BLASTP或TBLASTN(使用标准默认值)。任选地可以筛选BLAST结果。随后提交筛选结果和非筛选结果的全长序列以针对来自生物的序列进行反向BLAST(第二BLAST),其中查询序列来自所述的生物(其中在查询序列是SEQ ID NO:143或SEQ ID NO:144的情况下,第二BLAST因而将聚球藻属物种(Synechococcus sp.)PCC 7942)。随后比较第一和第二BLAST搜索的结果。若来自第一BLAST的高阶位命中源自与查询序列从其中衍生的物种相同的物种,反向BLAST随后理想地在最高命中的查询序列中产生,则鉴定到旁系同源物;若第一BLAST的中的高阶位命中不源自与查询序列从其中衍生的物种相同的物种,并且优选地在反向BLAST时产生属于最高命中之列的查询序列,则鉴定到直向同源物。
对于SHR,本发明通过用SEQ ID NO:208所示的核酸序列转化植物进行说明,其中所述核酸序列编码SEQ ID NO:209的多肽序列。然而,本发明的实施不限于这些序列;本发明的方法可以有利地使用如本文中定义的任意SHR编码核酸或SHR多肽实施。
对于SHR,编码SHR多肽的核酸的实例在本文实施例1的表A5中给出。此类核酸可用于实施本发明的方法。实施例1的表A5中给出的氨基酸序列是SEQ ID NO:209所示的SHR多肽的直向同源物和旁系同源物的序列实例,术语“直向同源物”和“旁系同源物”如文中所定义。其他直向同源物和旁系同源物可以通过开展所谓交互性blast搜索而容易地鉴定。这通常涉及第一BLAST,其中所述的第一BLAST包括提交查询序列(例如使用实施例1的表A5中列出的任一序列)用于针对任一序列数据库(如公众可用的NCBI数据库)的BLAST搜索。当从核苷酸序列开始时,一般使用BLASTN或TBLASTX(使用标准默认值),并且当从蛋白质序列开始时,可以使用BLASTP或TBLASTN(使用标准默认值)。任选地可以筛选BLAST结果。随后提交筛选结果和非筛选结果的全长序列以针对来自生物的序列进行反向BLAST(第二BLAST),其中查询序列来自所述的生物(其中在查询序列是SEQ IDNO:208或SEQ ID NO:209的情况下,第二BLAST因而将针对拟南芥序列)。随后比较第一和第二BLAST搜索的结果。若来自第一BLAST的高阶位命中源自与查询序列从其中衍生的物种相同的物种,反向BLAST随后理想地在最高命中的查询序列中产生,则鉴定到旁系同源物;若第一BLAST的中的高阶位命中不源自与查询序列从其中衍生的物种相同的物种,并且优选地在反向BLAST时产生属于最高命中之列的查询序列,则鉴定到直向同源物。
高阶位命中是具有低E-值的那些命中。E-值越低,评分越显著(或换句话说,偶然发现该命中的几率越低)。E-值的计算是本领域熟知的。除了E-值外,比较结果也由同一性百分数评分。同一性百分数指两个所比较的核酸(或多肽)序列之间特定长度范围内相同核苷酸(或氨基酸)的数目。在大型家族的情况下,可以使用ClustalW,随后使用邻接树法,以帮助观察相关基因的聚簇并鉴定直向同源物和旁系同源物。
核酸变体也可以用于实施本发明的方法。此类核酸变体的实例包括实施例1的表A中给出的核酸序列编码的氨基酸序列中任一个的同源物和衍生物的编码核酸,术语“同源物”和“衍生物”如文中所定义。也可用于本发明方法中的是实施例1的表A中给出的核酸序列编码的氨基酸序列中任一个的直向同源物或旁系同源物的同源物和衍生物的编码核酸。用于本发明方法中的同源物和衍生物与它们衍生自的未修饰的蛋白质具有基本相同的生物学活性和功能活性。
用于实施本发明方法的其他核酸变体包括编码TCP1或TCP2、底物蛋白-样、IPPT或SHR多肽的核酸的部分;与编码TCP1或TCP2、底物蛋白-样、IPPT或SHR多肽的核酸杂交的核酸;编码TCP1或TCP2、底物蛋白-样、IPPT或SHR多肽的核酸的剪接变体;编码TCP1或TCP2、底物蛋白-样、IPPT或SHR多肽的核酸的等位变体;以及通过基因改组获得的编码TCP1或TCP2、底物蛋白-样、IPPT或SHR多肽的核酸的变体。术语杂交序列、剪接变体、等位变体和基因改组如文中所述。
编码TCP1或TCP2、底物蛋白-样、或IPPT的核酸不需要是全长核酸,因为本发明方法的实施不依赖全长核酸序列的使用。本发明提供在植物中增强产量相关性状的方法,其包括在植物中引入和表达实施例1的表A中给出的核酸序列编码的核酸序列中任一个的部分,或实施例1中给出的核酸序列编码的任意氨基酸序列的直向同源物、旁系同源物或同源物的编码核酸的部分。
编码SHR多肽的核酸不需要是全长核酸,因为本发明方法的实施不依赖全长核酸序列的使用。本发明提供在亚最佳养分可利用性条件下培育的植物中增强产量相关性状的方法,其包括在植物中引入和表达实施例1的表A5中给出的核酸序列中任一个的部分,或实施例1的表A5中给出的任意氨基酸序列的直向同源物、旁系同源物或同源物的编码核酸的部分。本发明还涉及提高在无养分限制条件下培育的植物的千粒重(TKW)的方法,其包括在植物中引入和表达实施例1的表A5中给出的核酸序列中任一个的部分,或实施例1的表A5中给出的任意氨基酸序列的直向同源物、旁系同源物或同源物的编码核酸的部分。
核酸的部分可以例如通过对该核酸产生一个或多个缺失而制备。所述的部分可以以分离的形式加以使用或它们可以与其他编码性(或非编码性)序列融合,以便例如产生组合几种活性的蛋白质。当与其他编码序列融合时,翻译时产生的所得多肽可以比对该蛋白质部分所预测的多肽更大。
对于TCP1或TCP2,用于本发明方法中的部分编码文中定义的TCP1或TCP2多肽,其与实施例1中给出的核酸序列编码的氨基酸序列具有基本相同的生物学活性。优选地,此部分是在实施例1中给出的任何一种核酸的部分,或实施例1中给出的核酸序列编码的氨基酸序列中任一个的直向同源物或旁系同源物的编码核酸的部分。此部分的长度优选为至少500、550、600、650、700、750、800、850、900、950、100、1050、1100、1150、1200、1250、1300、1350、1400、1450、1500个连续核苷酸,其中所述的连续核苷酸是实施例1中给出的任何一种核酸序列,或是实施例1中给出的核酸序列编码的氨基酸序列中任一个的直向同源物或旁系同源物的编码核酸。最优选地,此部分是核酸SEQ ID NO:1或SEQ ID NO:3的部分。优选地,此部分编码下述氨基酸序列的片段,当其用于构建诸如图1或2所示的系统树时,与包含SEQ ID NO:2或SEQ ID NO:4所示的氨基酸序列的TCP1或TCP2多肽组聚簇,而不与任何其他组聚簇。
对于底物蛋白-样序列,用于本发明方法中的部分编码文中定义的底物蛋白-样多肽,其与实施例1的表A3中给出的氨基酸序列具有基本相同的生物学活性。优选地,此部分是在实施例1的表A3中给出的任何一种核酸的部分,或实施例1的表A3中给出的氨基酸序列中任一个的直向同源物或旁系同源物的编码核酸的部分。此部分的长度优选为至少500、600、700、800、900、1000、1100、1200、1300、1400、1500、1600、1700、1800、1850、1900、1950、2000、2050、2100、2150、2200、2250、2300个连续核苷酸,其中所述的连续核苷酸是实施例1的表A3中给出的任何一种核酸序列,或是实施例1的表A3中给出的氨基酸序列中任一个的直向同源物或旁系同源物的编码核酸。最优选地,此部分是核酸SEQ ID NO:43的部分。优选地,此部分编码下述氨基酸序列的片段,当其用于构建诸如图3(Holstein和Oliviusson,Protoplasma 226,13-21,2005)所示的系统树时,与包含SEQ ID NO:44所示的氨基酸序列的底物蛋白-样多肽组聚簇,而不与任何其他组聚簇。
对于IPPT,用于本发明方法中的部分编码文中定义的IPPT多肽,其与实施例1的表A4中给出的多肽序列具有基本相同的生物学活性。优选地,此部分是在实施例1的表A4中给出的任何一种核酸序列的部分,或实施例1的表A4中给出的多肽序列中任一个的直向同源物或旁系同源物的编码核酸序列的部分。优选地,此部分的长度按照递增的优选顺序为至少400、450、500、550、600、650、700、750、800、850、900、920个连续核苷酸,其中所述的连续核苷酸是实施例1的表A4中给出的任何一种核酸序列,或是实施例1的表A4中给出的多肽序列中任一个的直向同源物或旁系同源物的编码核酸序列。优选地,此部分是编码下述多肽序列的核酸序列的部分,该多肽序列按照递增的优选顺序,与SEQ ID NO:144所示的IPPT多肽或本文表A4中给出的任意多肽序列具有至少35%、40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性。最优选地,此部分是核酸序列SEQ ID NO:143的部分。
对于SHR,用于本发明方法中的部分编码文中定义的SHR多肽,其与实施例1的表A5中给出的氨基酸序列具有基本相同的生物学活性。优选地,此部分是在实施例1的表A5中给出的任何一种核酸的部分,或实施例1的表A5中给出的氨基酸序列中任一个的直向同源物或旁系同源物的编码核酸的部分。此部分的长度优选为至少1000,1250,1500,1600,1700个连续核苷酸,其中所述的连续核苷酸是实施例1的表A5中给出的任何一种核酸序列,或是实施例1的表A5中给出的氨基酸序列中任一个的直向同源物或旁系同源物的编码核酸。最优选地,此部分是核酸SEQ ID NO:208的部分。优选地,此部分编码下述氨基酸序列的片段,当其用于构建诸如图14所示的GRAS系统树时,与包含SEQ ID NO:209所示的氨基酸序列的SHR多肽组聚簇,而不与任何其他组聚簇。
用于本发明方法中的另一核酸变体是能够在降低的严格条件下、优选在严格条件下与编码如文中所定义的TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸杂交,或与如文中所定义的部分杂交的核酸。
本发明提供用于在植物中增强产量相关性状的方法,其包括在植物中引入并表达能够与实施例1的表A中给出的任何一种核酸杂交的核酸,或包括在植物中引入并表达如此一种核酸,其能够与实施例1的表A中给出的任意核酸序列编码的氨基酸的直向同源物、旁系同源物或同源物的编码核酸杂交的核酸。
对于TCP1或TCP2,或底物蛋白-样序列,用于本发明方法中的杂交序列编码文中定义的TCP1或TCP2多肽,或底物蛋白-样多肽,其与实施例1的表A中给出的核酸序列编码的氨基酸序列具有基本相同的生物学活性。优选地,杂交序列能够与实施例1的表A中给出的任一核酸杂交或与任意这些序列的部分杂交,其中所述的部分如上文所定义,或者其中杂交序列能够与实施例1的表A中给出的核酸序列编码的任一氨基酸序列的直向同源物或旁系同源物的编码核酸杂交。对于TCP1或CP2,最优选地,杂交序列能够与SEQ ID NO:1所示的核酸或其部分杂交,或与SEQ ID NO:3所示的核酸或其部分杂交。对于底物蛋白-样序列,最优选地,杂交序列能够与SEQ ID NO:43所示的核酸或其部分杂交。
对于IPPT,用于本发明方法中的杂交序列编码文中定义的IPPT多肽,其与实施例1的表A4中给出的多肽序列具有基本相同的生物学活性。优选地,杂交序列能够与实施例1的表A4中给出的任一核酸序列杂交或与任意这些序列的部分杂交,其中所述的部分如上文所定义,或者其中杂交序列能够与实施例1的表A4中给出的任一多肽序列的直向同源物或旁系同源物的编码核酸序列杂交。优选地,杂交序列优选能够与编码下述多肽序列的核酸序列杂交,该多肽序列按照递增的优选顺序,与SEQ ID NO:144所示的IPPT多肽或本文表A4中给出的任意多肽序列具有至少35%、40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性。最优选地,杂交序列能够与SEQ ID NO:143所示的核酸序列或其部分杂交。
用于本发明方法中的另一种核酸序列变体是编码如上文所定义的IPPT多肽的剪接变体,剪接变体如文中所定义。
对于SHR,用于本发明方法中的杂交序列编码文中定义的SHR多肽,其与实施例1的表A中给出的氨基酸序列具有基本相同的生物学活性。优选地,杂交序列能够与实施例1的表A中给出的任一核酸杂交或与任意这些序列的部分杂交,其中所述的部分如上文所定义,或者其中杂交序列能够与实施例1的表A中给出的任一氨基酸序列的直向同源物或旁系同源物的编码核酸序列杂交。最优选地,杂交序列能够与SEQ ID NO:208所示的核酸或其部分杂交。优选地,此杂交序列编码具有下述氨基酸序列的多肽,当其是全长,且用于构建诸如图14所示的GRAS系统树时,与包含SEQ ID NO:209所示的氨基酸序列的SHR多肽组聚簇,而不与任何其他组聚簇。
对于TCP1或TCP2,优选此杂交序列编码具有下述氨基酸序列的多肽,当其是全长,且用于构建诸如图1或2所示的系统树时,与包含SEQ ID NO:2或4所示的氨基酸序列的TCP1或TCP2多肽组聚簇,而不与任何其他组聚簇。
对于底物蛋白-样序列,优选此杂交序列编码具有下述氨基酸序列的多肽,当其是全长,且用于构建诸如图3(Holstein和Oliviusson,Protoplasma 226,13-21,2005)所示的系统树时,与包含SEQ ID NO:44所示的氨基酸序列的底物蛋白-样多肽组聚簇,而不与任何其他组聚簇。
用于本发明方法中的另一种核酸序列变体是编码如上文所定义的TCP1或TCP2,或底物蛋白-样,或SHR多肽的剪接变体,剪接变体如文中所定义。
本发明提供用于在植物中增强产量相关性状的方法,其包括在植物中引入并表达实施例1的表A中给出的任何一种核酸序列的剪接变体,或实施例1的表A中给出的核酸序列编码的任意氨基酸序列的直向同源物、旁系同源物或同源物的编码核酸的剪接变体。
对于SHR,也提供用于在植物中提高TKW的方法,其包括在植物中引入并表达实施例1的表A5中给出的任何一种核酸序列的剪接变体,或实施例1的表A5中给出的任意氨基酸序列的直向同源物、旁系同源物或同源物的编码核酸的剪接变体,且在无养分限制条件下培育植物。
对于TCP1或TCP2,优选的剪接变体是SEQ ID NO:1或3所示的核酸的剪接变体,或编码SEQ ID NO:2或4的直向同源物或旁系同源物的核酸的剪接变体。优选地,当编码此剪接变体的氨基酸序列用于构建诸如图1或2所示的系统树时,与包含SEQ ID NO:2或4所示的氨基酸序列的TCP1或TCP2多肽组聚簇,而不与任何其他组聚簇。
对于底物蛋白-样序列,优选的剪接变体是SEQ ID NO:43所示的核酸的剪接变体,或编码SEQ ID NO:44的直向同源物或旁系同源物的核酸的剪接变体。优选地,当编码此剪接变体的氨基酸序列用于构建诸如图3(Holstein和Oliviusson,Protoplasma 226,13-21,2005)所示的系统树时,与包含SEQ ID NO:44所示的氨基酸序列的底物蛋白-样多肽组聚簇,而不与任何其他组聚簇。
对于IPPT,优选的剪接变体是SEQ ID NO:143所示的核酸序列的剪接变体,或编码SEQ ID NO:44的直向同源物或旁系同源物的核酸序列的剪接变体。优选地,此剪接变体是编码下述多肽序列的核酸序列的剪接变体,该多肽序列按照递增的优选顺序,与SEQ ID NO:144所示的IPPT多肽或本文表A4中给出的任意多肽序列具有至少35%、40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性。
对于SHR,优选的剪接变体是SEQ ID NO:208所示的核酸的剪接变体,或编码SEQ ID NO:209的直向同源物或旁系同源物的核酸的剪接变体。优选地,当编码此剪接变体的氨基酸序列用于构建诸如图14所示的GRAS系统树时,与包含SEQ ID NO:209所示的氨基酸序列的SHR多肽组聚簇,而不与任何其他组聚簇。
用于实施本发明方法的另一种核酸变体是编码如上文所定义的TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸的等位变体,等位变体如文中所定义。
本发明提供用于在植物中增强产量相关性状的方法,其包括在植物中引入并表达实施例1的表A中给出的任何一种核酸的等位变体,或包括在植物中引入并表达实施例1的表A中给出的核酸序列编码的任意氨基酸序列的直向同源物、旁系同源物或同源物的编码核酸的等位变体。
对于IPPT,本发明提供用于增强产量相关性状的方法,其包括在植物种子中引入并表达实施例1的表A中给出的任何一种核酸的等位变体,或包括在植物种子中引入并表达实施例1的表A中给出的任意多肽序列的直向同源物、旁系同源物或同源物的编码核酸序列的等位变体。
对于SHR,也提供用于在植物中提高TKW的方法,其包括在植物中引入并表达实施例1的表A中给出的任何一种核酸的等位变体,或包括在植物中引入并表达实施例1的表A中给出的任意氨基酸序列的直向同源物、旁系同源物或同源物的编码核酸的等位变体,以及在无养分限制条件下培育植物。
对于TCP1或TCP2,用于本发明方法中的等位变体具有与TCP1或TCP2多肽SEQ ID NO:2或4,和实施例1中给出的核酸序列编码的任意氨基酸基本相同的生物学活性。等位变体天然存在,且包含在本发明方法中的是这些天然等位基因的用途。优选地,等位变体是SEQID NO:1或3的等位变体,或编码SEQ ID NO:2或4的直向同源物或旁系同源物的核酸的等位变体。优选地,当编码此剪接变体的氨基酸序列用于构建诸如图1或2所示的系统树时,与包含SEQ ID NO:2或4所示的氨基酸序列的TCP1或TCP2多肽组聚簇,而不与任何其他组聚簇。
对于底物蛋白-样序列,用于本发明方法中的等位变体具有与底物蛋白-样多肽SEQ ID NO:44,和实施例1的表A3中给出的任意氨基酸基本相同的生物学活性。等位变体天然存在,且包含在本发明方法中的是这些天然等位基因的用途。优选地,等位变体是SEQ ID NO:43的等位变体,或编码SEQ ID NO:44的直向同源物或旁系同源物的核酸的等位变体。优选地,当编码此剪接变体的氨基酸序列用于构建诸如图3(Holstein和Oliviusson,Protoplasma 226,13-21,2005)所示的系统树时,与包含SEQ ID NO:44所示的氨基酸序列的底物蛋白-样多肽组聚簇,而不与任何其他组聚簇。
对于IPPT,用于本发明方法中的等位变体具有与IPPT多肽SEQID NO:144,和实施例1的表A4中给出的任意氨基酸序列基本相同的生物学活性。等位变体天然存在,且包含在本发明方法中的是这些天然等位基因的用途。优选地,等位变体是SEQ ID NO:143的等位变体,或编码SEQ ID NO:144的直向同源物或旁系同源物的核酸序列的等位变体。优选地,该等位变体是下述多肽序列的等位变体,该多肽序列按照递增的优选顺序,与SEQ ID NO:144所示的IPPT多肽或本文表A4中给出的任意多肽序列具有至少35%、40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性。
对于SHR,用于本发明方法中的等位变体具有与SHR多肽SEQ IDNO:209,和实施例1的表A5中给出的任意氨基酸基本相同的生物学活性。等位变体天然存在,且包含在本发明方法中的是这些天然等位基因的用途。优选地,等位变体是SEQ ID NO:208的等位变体,或编码SEQ ID NO:209的直向同源物或旁系同源物的核酸的等位变体。优选地,当编码此剪接变体的氨基酸序列用于构建诸如图14所示的GRAS系统树时,与包含SEQ ID NO:209所示的氨基酸序列的SHR多肽组聚簇,而不与任何其他组聚簇。
基因改组或定向进化也可以用来产生编码如上文定义的TCP1或TCP2,或底物蛋白-样,或SHR多肽的核酸的变体;术语“基因改组”如文中所定义。
本发明提供用于在植物中增强产量相关性状或提高TKW性状的方法,其包括在植物中引入并表达实施例1的表A中给出的任何一种核酸序列的变体,或包括在植物中引入并表达如此核酸的变体,其中所述的核酸编码实施例1的表A中给出的核酸序列编码的任意氨基酸序列的直向同源物、旁系同源物或同源物,所述变体核酸通过基因改组获得,以及在无养分限制条件下培育植物。
对于TCP1或TCP2,优选地,通过基因改组获得的变体核酸编码下述氨基酸序列,当其用于构建诸如图1或2所示的系统树时,与包含SEQ ID NO:2或4所示的氨基酸序列的TCP1或TCP2多肽组聚簇,而不与任何其他组聚簇。
对于底物蛋白-样序列,优选地,通过基因改组获得的变体核酸编码下述氨基酸序列,当其用于构建诸如图3(Holstein和Oliviusson,Protoplasma 226,13-21,2005)所示的系统树时,与包含SEQ ID NO:44所示的氨基酸序列的底物蛋白-样多肽组聚簇,而不与任何其他组聚簇。
对于IPPT,优选地,通过基因改组获得的变体核酸序列编码下述多肽序列,该多肽序列按照递增的优选顺序,与SEQ ID NO:144所示的IPPT多肽或本文表A4中给出的任意多肽序列具有至少35%、40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性。
对于SHR,优选地,通过基因改组获得的变体核酸编码下述氨基酸序列,当其用于构建诸如图14所示的GRAS系统树时,与包含SEQID NO:209所示的氨基酸序列的SHR多肽组聚簇,而不与任何其他组聚簇。
另外,核酸变体也可以通过位点定向诱变获得。几种方法可用于实现位点定向诱变,最常见的是基于PCR的方法(Current Protocols inMolecular Biology.Wiley编)。
编码TCP1或TCP2多肽的核酸可以从任何天然来源或人工来源衍生。该核酸序列可以通过人类有意操作,在组成和/或基因组环境方面从其天然形式中修饰而来。优选地,编码TCP1或TCP2多肽的核酸来自植物,进一步优选地来自双子叶植物,更优选地来自苜蓿(Medicago)科,该核酸序列最优选地来自苜蓿(Medicago sativa)或蒺藜苜蓿(Medicago truncatula)。
编码底物蛋白-样多肽的核酸可以从任何任何天然或人工来源衍生。该核酸可以通过人类有意操作,在组成和/或基因组环境方面从其天然形式中修饰而来。优选地,编码底物蛋白-样多肽的核酸来自植物,进一步优选地来自双子叶植物,更优选地来自十字花科(Brassicaceae),最优选地该核酸来自拟南芥。
编码IPPT多肽的核酸序列可以从任何任何天然或人工来源衍生。该核酸可以通过人类有意操作,在组成和/或基因组环境方面从其天然形式中修饰而来。编码IPPT多肽的核酸序列来自原核生物(procaryota)界(domain),优选来自蓝藻(Cyanobacteria),进一步优选来自念珠藻目(Nostocales)、颤藻目(Oscillatoriales)、色球藻目(Chroococcales)、原绿菌目(Prochlorales)、Gloeobacterales、宽球藻目(Pleurocapsales)、真枝藻目(Stigonematales)。更优选地,编码IPPT多肽的核酸序列来自念珠藻属、束毛藻属(Trichodesmium)、鱼腥藻属(Anabaena)、Acaryochloris、微囊藻属(Microcystis)、嗜热蓝细菌属(Thermosynechococcus)、聚球藻属(Synechococcus)、原绿球藻属(Prochlorococcus)、粘杆菌属(Gloeobacter)、集胞藻属(Synechocystis)。最优选地,编码IPPT多肽的核酸序列来自聚球藻属物种,尤其来自聚球藻PCC 7942。
编码SHR多肽的核酸可以从任何任何天然或人工来源衍生。该核酸可以通过人类有意操作,在组成和/或基因组环境方面从其天然形式中修饰而来。优选地,编码SHR多肽的核酸来自植物,进一步优选地来自双子叶植物,更优选地来自十字花科,最优选地该核酸来自拟南芥。
本发明方法的实施产生了具有增强的产量相关性状的植物。特别地,本发明方法的实施产生这样的植物,其相对于对照植物具有提高的产量、尤其提高的种子产量。术语“产量”和“种子产量”在本文的“定义”部分中更详细地描述。
本文中对增强的产量相关性状的谈及意指植物的一个或多个部分的生物量(重量)增加,所述的部分可以包括地上(可收获)部分和/或地下(可收获)部分。特别地,此类可收获部分是营养性植物部分和/或种子,并且本发明方法的实施产生了相对于对照植物的种子产量而言,具有提高的种子产量的植物。
以谷物为例,产量提高可以表现为以下一个或多个指标:每平方米已建立的植物数目增加、每株植物花序数的提高、行数、每行核粒数、核粒重、千粒重、花序长度/直径的提高、种子饱满率(即饱满种子数除以种子总数并乘以100)提高,及其他。以稻为例,产量提高可以自身表现为下列一种或多种指标的提高:每平方米植物数、每株植物的花序数、每个花序的小穗数、每个花序的花(小花)数目(其表述为饱满种子数对原发花序数的比)、种子饱满率(即饱满种子数除以种子总数并乘以100)提高、千粒重提高及其他。
本发明提供相对于对照植物提高产量尤其是植物种子产量的方法,所述方法包括调节编码如本文定义的TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸在植物中的表达。
由于本发明的转基因植物具有提高的产量,故这些植物有可能在其生活周期的相应阶段(在其生活周期的至少部分期间)相对于对照植物的生长速率表现出提高的生长速率。
提高的生长速率可以是植物的一个或多个部分(包括种子)特有的,或可以基本上遍及整株植物。具有提高的生长速率的植物可以具备较短的生活周期。植物的生活周期可以意指从干燥成熟种子生长直至植物已经产生与起始材料相似的干燥成熟种子的阶段所需要的时间。这个生活周期可以受诸因素如早期生长势、生长速率、绿度指数、开花时间和种子成熟速度影响。生长速率的提高可以在植物生活周期的一个或多个阶段上或基本上在植物整个生活周期期间发生。在植物生活周期中早期期间提高的生长速率可以反映增强的生长势。生长速率的提高可以改变植物的收获周期,从而允许植物更晚地播种和/或更早地收获,而这本来是不可能的(可以随更早的开花时间获得相似效果)。如果大幅提高生长速率,则可以允许进一步播种相同植物物种的种子(例如播种并收获稻植物,随后播种并收获其他稻植物,全部稻植物均处于一个常规生长时期中)。类似地,如果大幅提高生长速率,则可以允许进一步播种不同植物物种的种子(例如播种并收获谷物植物,随后例如播种并任选收获大豆、马铃薯或任何其他合适的植物)。在一些作物植物的实例中,来自相同根状茎的额外收获次数也可以是可能的。改变植物的收获周期可以导致每平方米一年生物量生产提高(原因在于可以培育并收获任何具体植物的次数(即在一年中)提高)。生长速率的提高也可以允许将转基因植物在比其野生型对应物更广泛的地理区域内培育,因为培育作物的地域限制往往由栽种时期(早季)或在收获时期(晚季)的不利环境条件决定。如果缩短收获周期,则可以避开这类不利条件。生长速率可以通过从生长曲线衍生多个参数而确定,此类参数可以是:T-Mid(植物达到50%最大植物大小所花费的时间)和T-90(植物达到90%最大植物大小所花费的时间),以及其他。
根据本发明的一个优选特征,本发明方法的实施产生了相对于对照植物具有提高的生长速率的植物。因此,根据本发明,提供了用于提高植物生长速率的方法,所述方法包括在植物中调节编码如本文中定义的TCP1或TCP2,或底物蛋白-样,或SHR多肽的核酸表达。此外,提供了提高植物生长速率的方法,所述方法包括调节植物中编码如本文定义的SHR多肽的核酸表达,以及在亚最佳养分可利用性条件下培养植物。
通过实施本发明的方法,且在养分缺乏条件下、尤其在氮缺乏条件下培育植物,可获得增强的产量相关性状。养分缺乏可以因养分如氮、磷酸盐和其他含磷化合物、钾、钙、镉、镁、锰、铁和硼及其他元素缺少所致。根据本发明的优选特征,提供了用于增强植物中产量相关性状的方法,所述方法包括调节植物中编码SHR多肽的核酸表达和在氮缺乏条件下培育植物。
与对照植物相比,无论植物处于非胁迫条件下还是植物暴露于多种胁迫下,都发生产量和/或生长速率的增加。植物一般通过生长得更慢而对暴露于胁迫作出应答。在严重胁迫条件下,植物甚至可以完全停止生长。另一方面,轻微胁迫在本文中定义为植物对其暴露的任何胁迫,其中所述的胁迫未导致植物完全停止生长而没有恢复生长的能力。与非胁迫条件下的对照植物相比,轻微胁迫在本发明意义中导致受胁迫植物生长降低小于40%、35%或30%,优选小于25%、20%或15%,更优选小于14%、13%、12%、11%或10%或更低。由于农业实践(灌溉、施肥、杀虫剂处理)上的进步,在栽培作物植物中并不经常遇到严重胁迫。因此,由轻微胁迫诱导的受损生长往往是农业上不希望的特征。轻微胁迫是植物暴露的常见生物性和/或非生物性(环境)胁迫。非生物胁迫可以因干旱或水涝、厌氧胁迫、盐胁迫、化学毒性、氧化胁迫和热、寒冷或冰冻温度所致。非生物胁迫可以是因水胁迫(尤其因为干旱)、盐胁迫、氧化胁迫或离子胁迫引起的渗透胁迫。生物胁迫一般是由病原体如细菌、病毒、真菌、线虫和昆虫引起的那些胁迫。
特别地,本发明的方法可以在非胁迫条件下或在轻度干旱条件开展以产生相对于对照植物具有提高的产量的植物。如Wang等(Planta(2003)218:1-14)中报道,非生物性胁迫导致一系列不利地影响植物生长及生产力的形态学、生理学、生物化学和分子变化。干旱、盐度、极端温度和氧化胁迫已知相互联系并可以通过相似的机制诱导生长损害及细胞损害。Rabbani等(Plant Physiol(2003)133:1755-1767)描述了干旱胁迫与高盐度胁迫之间极高程度的“交互作用”。例如,干旱和/或盐化作用主要表现为渗透胁迫,从而导致细胞内稳态和离子分布的破坏。经常伴随高温或低温、盐度或干旱胁迫的氧化胁迫可以引起功能蛋白和结构蛋白变性。因此,这些多样的环境胁迫常常激活相似的细胞信号传导途径和细胞应答,如产生胁迫蛋白、上调抗氧化物质、积累兼容性溶质和生长停滞。如本文所用的术语“非胁迫”条件是允许植物最佳生长的那些环境条件。本领域技术人员清楚对于给定地点的正常土壤条件和气候条件。
对于IPPT,由于多样的环境胁迫激活相似途径,故本发明采用干旱胁迫的示例不应当视为限于干旱胁迫,而更应视为一种筛选法以相对于可比较胁迫条件下、通常在非生物胁迫下培育的对照植物,说明如上文定义的IPPT多肽参与提高产量相关性状。
对于IPPT,如本文中定义的术语“非生物胁迫”意指以下任意一项或多项:水胁迫(因干旱或过量的水所致)、缺氧胁迫、盐胁迫、温度胁迫(因热、寒冷或冰冻温度所致)、化学毒性胁迫和氧化胁迫。根据本发明的一个方面,所述非生物胁迫是渗透胁迫,其选自水胁迫、盐胁迫、氧化胁迫和离子胁迫。优选地,水胁迫是干旱胁迫。术语“盐胁迫”不限于普通盐(NaCl),不过可以是由以下一种或多种盐:NaCl、KCl、LiCl、MgCl2、CaCl2等引起的任意胁迫。
相对于可比较条件下培育的对照植物,本发明方法的实施赋予在非胁迫条件下或在轻度干旱条件下培育的植物提高的产量。因此,根据本发明,提供了用于在非胁迫条件下或在轻度干旱条件下培育的植物中提高产量的方法,所述方法包括增加植物中编码TCP1或TCP2,或底物蛋白-样多肽的核酸表达。
相对于可比较条件下培育的对照植物,本发明方法的实施赋予在养分缺乏条件下、尤其在缺氮条件下培育的植物提高的产量。因此,根据本发明,提供了用于在养分缺乏条件下培育的植物中提高产量的方法,所述方法包括调节植物中编码TCP1或TCP2,或底物蛋白-样多肽的核酸表达。养分缺乏可以因养分如氮、磷酸盐和其他含磷化合物、钾、钙、镉、镁、锰、铁和硼及其他元素缺少所致。
对于IPPT,相对于可比较胁迫条件下培育的对照植物,本发明方法的实施产生在非生物胁迫条件下具有增强的产量相关性状的植物。因此,根据本发明,提供了用于提高非生物胁迫条件下生长的植物中产量相关性状的方法,所述方法包括增加植物种子中编码IPPT多肽的核酸序列表达。根据本发明的一个方面,所述非生物胁迫是渗透胁迫,其选自以下一种或多种胁迫:水胁迫、盐胁迫、氧化胁迫和离子胁迫。
另外,对于IPPT,本发明方法的实施产生在养分可利用性降低、尤其氮可利用性降低的条件下培育的植物,该植物相对于可比较条件下培育的对照植物具有增强的产量相关性状。因此,根据本发明,提供了用于提高在降低的养分可用性、优选降低的氮可用性条件下生长的植物中产量相关性状的方法,所述方法包括增加植物种子中编码IPPT多肽的核酸序列表达。降低的养分可利用性可以因养分如氮、磷酸盐和其他含磷化合物、钾、钙、镉、镁、锰、铁和硼及其他养分匮乏或过多所致。优选地,降低的养分可利用性是降低的氮可利用性。
非生物的环境胁迫的另一个例子是需要被所述植物同化用于生长和发育的一种或多种养分的可利用性降低。因为养分利用效率强烈影响植物产量和产品品质,故向田地倾注大量肥料以优化植物生长和产品品质。植物的生产量通常受3种主要养分磷、钾和氮限制,这3种养分中,氮通常是是植物生长的限速性元素。因此,植物生长所需的主要营养元素是氮(N)。氮是活细胞中存在的许多重要化合物的组成成分,所述的重要化合物包括氨基酸、蛋白质(酶)、核酸和叶绿素。1.5%至2%的植物干物质是氮而大约16%的植物总蛋白是氮。因此,氮可利用性是作物植物生长和生产的主要限制性因素(Frink等(1999)Proc NatlAcad Sci USA 96(4):1175-1180),并且也对蛋白质积累和氨基酸组成产生重大影响。因此,在氮限制性条件下生长时具有增强的产量相关性状的植物是意义重大的。
本发明包括通过本发明方法可获得的植物或其部分(包括种子)或其细胞。所述植物或其部分或其细胞包含编码如上文所定义TCP1或TCP2,或底物蛋白-样,或SHR多肽的核酸转基因。对于IPPT,植物或其部分或其细胞包含编码选自如上文所定义IPPT多肽的核酸转基因,与种子特异性启动子有效连接。
本发明也提供了基因构建体和载体以促进在植物中导入和/或表达编码TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸。所述基因构建体可以插入适于转化至植物并适于在转化细胞中表达目的基因的载体,所述载体可以是市售的。本发明也提供了如本文中定义的基因构建体在本发明方法中的用途。
更具体地,本发明提供了构建体,其包含:
(a)编码如上定义的TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸;
(b)能够驱动(a)的核酸序列表达的一个或多个调控序列;和任选地
(c)转录终止序列。
优选地,编码TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸如上文定义。术语“调控序列”和“终止序列”如本文中定义。
植物用包含上述任意核酸的载体转化。技术人员非常了解必须存在于所述载体上以便成功转化、选择和增殖含有目的序列的宿主细胞的遗传元件。此目的序列有效地与一个或多个调控序列(至少与启动子)连接。
有利地,任意类型的启动子,无论是天然的或合成的,可以用来驱动所述核酸序列的表达。组成型启动子是在所述方法中特别有用的。优选地,所述组成型启动子也是遍在启动子。对于IPPT,种子特异性启动子在本方法中尤其有用。例如用于叶、茎、块茎、分生组织的优选的表达的其他组织特异性启动子在实施本发明方法中尤其有用。发育调节性启动子也可用于实施本发明的方法。对于多种启动子类型的定义,见本文中的“定义”部分。对于多种启动子类型的定义,见本文中的“定义”部分。
应当明白本发明的应用不限于SEQ ID NO:1或3所示的编码TCP1或TCP2多肽的核酸,本发明的应用也不限于编码TCP1或TCP2多肽的核酸在受组成型启动子驱动时的表达。
对于TCP1或TCP2,组成型启动子优选是GOS2启动子,优选来自稻的GOS2启动子。进一步优选地,该组成型启动子基本上是与SEQID NO:5相似的核酸序列所示的,最优选该组成型启动子是SEQ IDNO:5所示的(对于组成型启动子的其他例子,见本文“定义”部分中的表2b)。根据另一优选实施方案,该组成型启动子优选为高迁移率族蛋白(HMG)启动子。进一步优选地,该组成型启动子基本上是与SEQID NO:6相似的核酸序列所示的,最优选该组成型启动子是SEQ IDNO:6所示的。
还应当明白本发明的应用不限于SEQ ID NO:43所示的编码底物蛋白-样多肽的核酸,本发明的应用也不限于编码底物蛋白-样多肽的核酸在受组成型启动子驱动时的表达。
此外,应当明白本发明的应用不限于SEQ ID NO:143所示的编码IPPT多肽的核酸序列,本发明的应用也不限于编码IPPT多肽的核酸序列在受种子特异性启动子驱动时的表达。
还应当明白本发明的应用不限于SEQ ID NO:208所示的编码SHR多肽的核酸,本发明的应用也不限于编码SHR多肽的核酸在受组成型启动子驱动时的表达。
对于底物蛋白-样序列,组成型启动子优选为GOS2启动子,优选来自稻的GOS2启动子。进一步优选地,该组成型启动子由基本上与SEQ ID NO:45相似的核酸序列代表,最优选该组成型启动子SEQ IDNO:45所代表。对于组成型启动子的其他例子,见本文“定义”部分中的表2。
对于IPPT,优选地,一种构建体调控序列是种子特异性启动子。种子特异性启动子的实例是脱水蛋白启动子,优选稻脱水蛋白启动子,更优选SEQ ID NO:204所示的脱水蛋白启动子。或者,种子特异性启动子是蛋白酶抑制剂启动子,优选稻蛋白酶抑制剂启动子,更优选SEQ ID NO:205所示的蛋白酶抑制剂启动子。
任选地,一个或多个终止子序列可用于被引入植物中的构建体。其他调节元件可以包括转录增强子以及翻译增强子。本领域技术人员将知道可能适用于实施本发明的终止子和增强子序列。如定义部分中描述,内含子序列也可以添加至5’非翻译区(UTR)或编码序列中,以提高细胞质中积累的成熟信使的量。(除启动子、增强子、沉默子、内含子序列、3’UTR和/或5’UTR区域之外的)其他调控序列可以是蛋白质/或RNA稳定化元件。此类序列将是已知的或可以由本领域技术人员轻易地获得。
本发明的基因构建体可以还包括对于特定细胞类型中维持和/或复制所需要的复制起点序列。一个例子是当需要基因构建体作为游离型遗传元件(例如质粒或粘粒分子)在细菌细胞中维持时的复制起点。优选的复制起点包括但不限于f1-ori和colE1。
为检测如用于本发明方法中的核酸序列的成功转移和/或选择包含这些核酸的转基因植物,使用标记基因(或报道基因)是有利的。因此,所述基因构建体可以任选地包含选择性标记基因。选择性标记在本文的“定义”部分中更详细地描述。一旦不再需要所述标记基因时,可以从转基因细胞中移除或切除它们。用于标记移除的技术是本领域已知的,有用的技术在上文定义部分中描述。
本发明也提供了用于产生转基因植物的方法,所述转基因植物相对于对照植物具有增强的产量相关性状,其中所述方法包括在植物中引入并表达编码如上文所定义的TCP1或TCP2,或底物蛋白-样多肽的任意核酸。
本发明也提供了用于产生转基因植物的方法,所述转基因植物相对于对照植物具有增强的产量相关性状,其中所述方法包括在植物中引入并表达编码如上文所定义的SHR多肽的任意核酸,以及在亚最优养分可利用性条件下培育植物。
更具体地,本发明提供了用于产生转基因植物的方法,所述转基因植物具有增加的增强产量相关性状、尤其提高的(种子)产量,其中所述方法包括:
(i)在植物或植物细胞中引入并表达编码TCP1或TCP2多肽的核酸;和
(ii)在促进植物生长和发育的条件下培育植物细胞。
(i)的核酸可以是能够编码如本文中所定义的TCP1或TCP2多肽的任意核酸。
对于底物蛋白-样多肽,本发明提供了用于产生转基因植物的方法,所述转基因植物具有增加的增强产量相关性状、尤其提高的生物量和或提高的种子产量,其中所述方法包括:
(i)在植物或植物细胞中引入并表达编码底物蛋白-样多肽的核酸;和
(ii)在促进植物生长和发育的条件下培育植物细胞。
(i)的核酸可以是能够编码如本文中所定义的底物蛋白-样多肽的任意核酸。
在另一实施方案中,本发明提供了用于产生转基因植物的方法,所述转基因植物相对于对照植物具有增强的产量相关性状,其中所述方法包括在植物种子中引入并表达编码如上文所定义的IPPT多肽的任意核酸。
对于IPPT,更具体地,本发明提供了用于产生转基因植物的方法,所述转基因植物相对于对照植物具有增强的产量相关性状,其中所述方法包括:
(i)在种子特异性启动子调控下,在植物、植物部分、或植物细胞中引入并表达编码IPPT多肽的核酸序列;和
(ii)在促进植物生长和发育的条件下培育植物细胞、植物部分或植物。
(i)的核酸序列可以是能够编码如本文中所定义的IPPT多肽的任意核酸序列。
在又一实施方案中,本发明提供了用于产生转基因植物的方法,所述转基因植物具有增强的产量相关性状、尤其提高的(种子)产量,其中所述方法包括:
(i)在植物或植物细胞中引入并表达编码SHR多肽的核酸;和
(ii)在亚最优养分可利用性条件下培育植物细胞。
对于SHR,本发明提供了用于产生转基因植物的方法,所述转基因植物相对于对照植物具有增加的TKW,其中所述方法包括在植物中引入并表达编码如上文所定义的SHR多肽的任意核酸和在无养分限制条件下培育植物细胞。更具体地,本发明提供了用于产生转基因植物的方法,所述转基因植物具有增加的TKW,其中所述方法包括:
(i)在植物或植物细胞中引入并表达编码IPPT多肽的核酸;和
(ii)在无养分限制条件下培育植物细胞。
(i)的核酸可以是能够编码如本文中所定义的SHR多肽的任意核酸。
该核酸可以直接地导入植物细胞或导入植物自身(包括导入组织、器官或植物的任何其他部分)。根据本发明的优选特征,该核酸优选地通过转化导入植物。术语“转化”在本文的“定义”部分中更详细地描述。
基因修饰的植物细胞可以借助技术人员熟悉的全部方法再生。合适的方法可见于上文提及的S.D.Kung和R.Wu,Potrykus或和Willmitzer的出版物。
通常在转化后,对植物细胞或细胞群体选择一个或多个标记的存在性,其中所述标记由随同目的基因一起共转移的植物可表达基因编码,随后将转化材料再生成完整植物。为了选择转化的植物,一般使转化中获得的植物材料经历选择条件,从而转化植物可以与非转化植物区分开。例如,以上述方式获得的种子可以播种,并且在初始培育时间后,通过喷洒接受合适的选择。另一种可能性在于种子根据需要消毒后,在使用合适选择剂的琼脂板上培育,从而仅转化的种子可以长成植物。备选地,筛选所述转化植物的选择性标记(如上文所述的选择性标记)的存在性。
在DNA转移和再生后,推定转化的植物也可以例如使用DNA印迹分析对目的基因的存在性、拷贝数和/或基因组构造进行评价。备选地或额外地,新导入的DNA的表达水平可以使用RNA印迹分析和/或蛋白质印迹分析监测,这两项技术均是本领域普通技术人员熟知的。
产生的转化植物可以通过多种方法繁殖,如通过克隆繁殖法或经典育种技术。例如,第一世代(或T1)转化植物可以进行自交并且选择纯合的第二世代(或T2)转化体,并且T2植物随后可以通过经典育种技术进一步繁殖。产生的转化生物可以采取多种形式。例如,它们可以是转化细胞和非转化细胞的嵌合体;克隆性转化体(例如,被转化以含有表达盒的全部细胞);转化组织和非转化组织的移植体(例如在植物中,与未转化接穗嫁接的转化根状茎)。
本发明明确地扩展至由本文中所述的任意方法产生的任意植物细胞或植物,并扩展至全部植物部分及其繁殖体。本发明进一步扩展以包括已经由前述任意方法产生的原代转化或转染细胞、组织、器官或完整植物的子代,唯一要求是子代表现与如本发明方法中的亲本相同的基因型和/或表型特征。
本发明也包括宿主细胞,其包含编码如上文所定义TCP1或TCP2,或底物蛋白-样,或SHR多肽的分离的核酸。本发明的优选宿主细胞是植物细胞。对于IPPT,本发明也包括宿主细胞,其包含编码如上文所定义的IPPT多肽的分离的核酸序列,其与种子特异性启动子有效连接。对于本发明方法中所用核酸或载体、表达盒或构建体或载体的宿主植物原则上有利地是能够合成在本发明方法中所使用的多肽的全部植物。
本发明的方法有利地适用于任意植物。特别用于本发明方法中的植物包括属于植物界超家族、尤其属于单子叶和双子叶植物的全部植物,包括饲用或饲料豆科植物、观赏植物、粮食作物、树或灌木。根据本发明的一个优选实施方案,所述植物是作物植物。作物植物的实例包括大豆、向日葵、卡诺拉油菜、苜蓿、油菜籽、棉花、番茄、马铃薯和烟草。更优选地,所述植物是单子叶植物。单子叶植物的实例包括甘蔗。更优选地,所述植物是谷物植物。谷物植物的实例包括稻、玉米、小麦、大麦、稷、黑麦、小黑麦、高粱和燕麦。
本发明也扩展至植物的可收获部分,例如但不限于种子、叶、果实、花、茎、根、根状茎、块茎和球茎。本发明进一步涉及衍生自、优选直接衍生自此种植物的可收获部分的产品,如干燥颗粒或粉末、油、脂肪和脂肪酸、淀粉或蛋白质。
关于IPPT,本发明也扩展至植物的可收获部分,所述植物包含编码与种子特异性启动子有效链接的IPPT(如上文定义)的分离核酸序列,例如但不限于种子、叶、果实、花、茎、根状茎、块茎和球茎。本发明进一步涉及衍生自,优选直接衍生自此种植物的可收获部分的产品,如干燥颗粒或粉末、油、脂肪和脂肪酸、淀粉或蛋白质。用于增加核酸序列或基因或基因产物表达的方法是本领域中充分记载的并且在定义部分中提供了实例。
根据本发明的优选特征,调节的表达是提高的表达。用于增加核酸或基因或基因产物表达的方法是本领域中充分记载的并且在定义部分中提供了实例。
如上所述,用于调节编码TCP1或TCP2,或底物蛋白-样,或SHR多肽的核酸的优选方法是在植物中引入并表达编码TCP1或TCP2,或底物蛋白-样,或SHR多肽的核酸;或用于增加编码IPPT多肽的核酸序列的优选方法是在植物种子中引入并表达编码IPPT多肽的核酸序列;然而,也可以使用包括但不限于T-DNA活化标签技术、TILLING、同源重组在内的其他熟知技术实现实施本方法的效果,即增强产量相关性状。在定义部分中提供了这些技术的描述。
本发明也包括编码如文中所述TCP1或TCP2,或底物蛋白-样,或SHR多肽的核酸的用途,和这些TCP1或TCP2,或底物蛋白-样,或SHR多肽在增强植物中任一前述产量相关性状的用途。本发明也包括编码如文中所述IPPT多肽的核酸序列,和这些IPPT多肽在正常生长条件下、在非生物胁迫生长条件(优选地在渗透胁迫生长条件)下和在养分可利用性降低的条件下、优选在氮可利用性降低的生长条件下增强植物中任一前述产量相关性状的用途。
编码本文中所述TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸;或TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽自身可以用于其中鉴定到DNA标记的育种程序中,其中所述的DNA标记可以遗传地与TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽编码基因连锁。所述核酸/基因或TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽自身可以用来定义分子标记。这种DNA或蛋白质标记随后可以在育种程序中用来在本发明的方法中选择具有如上文所定义增强的产量相关性状、或增加的TKW的植物。
编码TCP1或TCP2,或底物蛋白-样,或SHR多肽的核酸/基因的等位变体也可以用于标记辅助的育种程序中。此类育种程序有时需要使用例如EMS诱变法通过对植物进行诱变处理而导入等位变异;备选地,所述程序可以从收集并非故意造成的所谓“自然”源性等位变体开始。随后进行等位变体的鉴定,例如通过PCR法。此后是步骤:选择所讨论的和导致产量提高的序列的优异等位变体。一般通过监测含有所讨论序列的不同等位变体的植物的生长性能实施选择。可以在温室中或在田间监测生长性能。其他任选步骤包括将其中鉴定到优异等位变体的植物与另一种植物杂交。这可能用来例如产生感兴趣的表型特征的组合。
编码IPPT多肽的基因/核酸序列的等位变体也可以用于标记辅助的育种程序中。此类育种程序有时需要使用例如EMS诱变法通过对植物进行诱变处理而导入等位变异;备选地,所述程序可以从收集并非故意造成的所谓“自然”源性等位变体开始。随后进行等位变体的鉴定,例如通过PCR法。此后是步骤:选择所讨论的和导致产量提高的序列的优异等位变体。一般通过监测含有所讨论序列的不同等位变体的植物的生长性能实施选择。可以在温室中或在田间监测生长性能。其他任选步骤包括将其中鉴定到优异等位变体的植物与另一种植物杂交。这可能用来例如产生感兴趣的表型特征的组合。
编码TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸也可以作为探针用于遗传地或物理地绘制所述探针构成其一部分的基因并且用作与这些基因连锁的性状的标记。此类信息可以用于植物育种中,以开发具有所希望表型的品系。编码TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸的这种用途仅需要具有至少15个核苷酸长度的核酸序列。编码TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸可以用作限制性片段长度多态性(RFLP)标记。限制性消化的植物基因组DNA的DNA印迹物(Sambrook J,Fritsch EF和Maniatis T(1989)Molecular Cloning,A LaboratoryManual)可以用编码TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸探测。所得的结合图式随后可以使用计算机程序如MapMaker(Lander等,(1987)Genomics 1:174-181)开展遗传分析以构建遗传图。此外,所述核酸可以用来探测含有一组个体的限制性核酸内切酶处理的基因组DNA的DNA印迹物,其中所述的一组个体代表具有定义的遗传杂交的亲本和子代。DNA多态性的分离是明显的并用来计算编码TCP1或TCP2,或底物蛋白-样,或IPPT,或SHR多肽的核酸在先前使用这个群体获得的遗传图中的位置(Botstein等,(1980)Am.J.Hum.Genet.32:314-331)。
在Bernatzky和Tanksley(1986)Plant Mol.Biol.Reporter 4:37-41中描述了植物基因来源的探针的产生及其在遗传作图中的用途。许多出版物描述了使用上文概述的方法学或其变例对特定cDNA克隆的遗传作图。例如,F2互交群、回交群、随机交配群、邻近纯合系和其他个体群体可以用于作图。此类方法学是本领域技术人员熟知的。
所述核酸探针也可以用于物理作图(即序列在物理图上的排列;见Hoheisel等在:Non-mammalian Genomic Analyasis:A Practical Guide,Academic press 1996,第319-346页及其中引用的参考文献)。
在另一个实施方案中,所述核酸探针可以在直接荧光原位杂交(FISH)作图法(Trask(1991)Trends Genet.7:149-154)中使用。尽管当前的FISH作图法支持使用大的克隆(几个kb至几百个kb;见Laan等,(1995)Genome Res.5:13-20),然而灵敏度的改善可以允许使用更短探针进行FISH作图。
用于遗传作图及物理作图的多种基于核酸扩增的方法可以使用所述核酸而实施。方法例子包括等位基因特异性扩增法(Kazazian(1989)J.Lab.Clin.Med 11:95-96)、PCR扩增片段的多态性(CAPS;Sheffield等,(1993)Genomics 16:325-332)、等位基因特异性连接(Landegren等,(1988)Science 241:1077-1080)、核苷酸延伸反应(Sokolov(1990)NucleicAcid Res.18:3671)、放射杂交作图(Walter等,(1997)Nat.Genet.7:22-28)和Happy作图法(Dear和Cook(1989)Nucleic Acid Res.17:6795-6807)。对于这些方法,使用一种核酸序列的序列来设计并产生在扩增反应或在引物延伸反应中使用的引物对。此类引物的设计是本领域技术人员熟知的。在使用基于PCR遗传作图的方法中,可能需要在对应于当前核酸序列的区域中鉴定作图交叉的亲本之间的DNA序列差异。然而,这对作图法而言通常不是必需的。
如前文所述,本发明方法产生了具有增强的产量相关性状的植物。这些性状也可以与经济上有利的其他性状组合,如其他的产量增强性状、其他非生物胁迫和生物胁迫耐受性、除草剂,杀虫剂耐受性、调节多种构造性特征和/或生物化学特征和/或生理学特征的性状。
附图简述
本发明现在将参考以下附图进行描述,其中:
图1为TCP1多肽的多重对比,加框标出的TCP结构域和结构域A、B和C。
图2为TCP2多肽的多重对比,加框标出的TCP结构域和结构域1、2、3、4和5。
图3代表SEQ ID NO:44的结构域结构,如SMART中识别出的ENTH结构域以粗体标出,保守基序1至5是下划线的。
图4代表多种底物蛋白-样蛋白序列的多重比对。数据库登录号用作鉴定物。
图5显示包括ENTH和ANTH结构域的真核蛋白的系统树(Holstein和Oliviusson 2005)。使用ClustalW 1.82比对所述蛋白质的氨基端部分(200个氨基酸)且结果用于DrawTree(PHYLIP包)。SEQ IDNO:44与植物ENTH组聚簇。
图6代表双元载体,其用于在稻中增加稻GOS2启动子(pGOS2)控制下的底物蛋白-样编码核酸的表达。
图7详述了用于实施本发明方法的序列的实例。
图8图示了两种主要的细胞激动素生物合成途径:(a)腺苷酸-IPT途径,使用AMP、ADP或ATP,以及DMAPP或HMBDP,和(b)tRNA-IPT途径,使用tRNA和DMAPP。根据Yevdakova和vonSchwartzenberg(2007)Planta 226:683-695。
图9显示异戊二烯化细胞激动素生物合成途径的具体模式,根据akakibara(2006)Annu Rev Plant Biol 57.431-449。tRNA-IPT在右上角通过黑色箭头指示,该途径的特定最终细胞激动素产物(cZ)在右下角也通过黑色箭头指示。
图10显示表A4的IPPT多肽的AlignX(来自Vector NTI 10.3,Invitrogen Corporation)序列多重比对。加框标出SEQ ID NO:199所示的N端ATP/GTP结合位点基序A(P-环)、SEQ ID NO:200所示的保守基序ⅠDSR(Q/L)(V/L/I)、SEQ ID NO:201所示的保守基序Ⅱ(N/D/S/T)(I/V)GTAKP(T/S)、SEQ ID NO:202所示的保守基序ⅢL(V/A/I)GG(S/T)GLY、和保守基序ⅣF/Y/L)AK(R/K/Q)Q(R/K/M)TWFR。在真核tRNA-IPT中发现的假定的锌指保守基序C2H2(C-X2-C-X(12,18)-H-X5-H以括号标出,并加框标出保守Cys和His残基。
图11代表双元载体,其用于在稻的种子中增加由种子特异性脱水蛋白启动子、或者由来自稻的种子特异性蛋白酶抑制剂启动子的控制下的IPPT多肽编码核酸序列的表达。
图12详述了用于实施本发明方法的序列的实例。
图13显示具有5个基序的GRAS蛋白质的结构。
图14GRAS和SHR蛋白质的邻接树。使用MUSCLE比对来自稻、拟南芥的GRAS蛋白质,和来自多种组织的SHR相关蛋白质。用CLUSTALX产生邻接树。进行100次重复bootstrap分析。显示主要节点的bootstrap支持值。指出SHR相关蛋白质。A.thaliana:拟南芥;E.grandis:巨桉(Eucalyptus grandis);G.max:大豆(Glycine max);L.sativa:生菜(Latuca sativa);M trucatula:蒺藜苜蓿;O.sativa:稻(Oryza sativa);P.taeda:火炬松(Pinus taeda);P.patens:展叶剑叶藓(Physcomitrella patens);P.trichocarpa:毛果杨(Populustrichocarpa);R.communis:蓖麻(Ricinus communis);S.tuberosum:马铃薯(Solanum tuberosum);V.vinifera:葡萄(Vitis vinifera);Z.mays:玉蜀黍(Zea mays);-part:部分序列。
图15显示GRAS家族成员与水平线上方输入所示SHR家族成员的序列同一性百分比。SHR分支在包括藓类和裸子植物的陆生植物中高度保守。相对于其他分支成员低于33%的同一性,该分支中的SHR蛋白质相互之间具有超过41%的同一性。
图16代表双元载体,其用于在稻中增加稻GOS2启动子(pGOS2)控制下的SHR编码核酸的表达。
图17详述了用于实施本发明方法的序列的实例。
在一个实施方案中,本发明涉及如下概括的主题:
项1:用于相对于对照植物增强植物中产量相关性状的方法,其包括包含调节编码TCP1或TCP2多肽的核酸在植物中的表达,所述TCP1多肽包含:
(i)与图1所示任一序列的TCP结构域以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的TCP结构域;和
(ii)与图1所示任一序列的结构域A以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iii)与图1所示任一序列的结构域B以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iv)与图1所示任一序列的结构域C以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域,
且所述TCP2多肽包含:
(i)与图2所示任意序列的TCP结构域以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的TCP结构域;和
(ii)与图2所示任意序列的结构域1以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iii)与图2所示任意序列的结构域2以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iv)与图2所示任意序列的结构域3以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域。
项3:根据项1或2的方法,其中所述TCP1多肽在构建诸如图1所示的TCP系统树时,倾向于与包含SEQ ID NO:2所示的多肽序列的TCP进化支聚簇,而不与任何其他TCP进化支聚簇。
项4:根据项1或2的方法,其中所述TCP2多肽在构建诸如图2所示的TCP系统树时,倾向于与包含SEQ ID NO:4所示的多肽序列的TCP进化支聚簇,而不与任何其他TCP进化支聚簇。
项5:根据任意前述项的方法,其中所述核酸序列编码SEQ ID NO:2或4的直向同源物或旁系同源物。
项6:根据任意前述项的方法,其中所述调节的表达是编码TCP1或TCP2多肽的核酸增加的表达。
项7:根据项6的方法,其中所述增加的表达通过T-DNA活化标签技术、TILLING或同源重组中的一种或多种实现。
项8:根据项6的方法,其中所述增加的表达通过在植物中引入和表达编码TCP1或TCP2多肽的核酸序列实现。
项9:根据任意前述项的方法,其中所述增强的产量相关性状包括相对于对照植物而言的提高的种子重量。
项10:根据项8或9的方法,其中所述核酸序列与组成型启动子有效连接,优选与HMGP(高迁移率族蛋白)启动子或至GOS2启动子有效连接。
项11:根据项7至9中任一项的方法,其中所述编码TCP1或TCP2多肽的核酸序列优选是植物来源的,进一步优选来自双子叶植物,更优选来自苜蓿科,最优选来自苜蓿或蒺藜苜蓿。
项12:由根据项1至11中任一项的方法可获得的植物、其部分(包括种子),其中所述的植物或其部分包含编码TCP1或TCP2多肽的核酸转基因。
项13:构建体,其包含:
(i)编码TCP1或TCP2多肽的核酸序列;
(ii)能够驱动(i)的核酸序列表达的一个或多个调控序列;和任选地
(iii)转录终止序列。
项14:根据项13的构建体,其中所述一个或多个调控序列至少是组成型启动子,优选是HMGP或GOS2启动子。
项15:根据项13或14的构建体在制备植物中的用途,所述植物相对于对照植物具有提高的产量,尤其具有提高的种子产量。
项16:用根据项13或14的构建体转化的植物、植物部分或植物细胞。
项17:用于制备相对于对照植物而言具有提高的种子产量的转基因植物的方法,所述方法包括:
(i)在植物或植物细胞中引入并表达编码TCP1或TCP2多肽的核酸序列;和
(ii)在促进植物生长和发育的条件下培育所述植物细胞。
项18:转基因植物,其相对于对照植物而言具有因编码TCP1或TCP2多肽的核酸的表达增加引起的提高的产量,尤其提高的种子产量,或者从所述转基因植物衍生的转基因植物细胞。
项19:根据项18的转基因植物,其中所述提高的种子产量是以下一项或多项:(i)提高的种子重量;(ii)提高的收获指数;(iii)提高的千粒重;(iv)提高的每穗花数;(v)提高的饱满率;或(vii)提高的饱满种子数。
项20:根据项12、16、18或19的转基因植物或从所述转基因植物衍生的转基因植物细胞,其中所述的植物是作物植物或单子叶植物或谷物植物,如稻、玉米、小麦、大麦、粟、黑麦、高粱和燕麦。
项21:根据项20的植物的可收获部分,其中所述的可收获部分优选是种子。
项22:产物,其衍生自根据项20的植物和/或根据项21的植物的可收获部分。
项23:编码TCP1或TCP2多肽的核酸在提高植物的产量、尤其是种子产量中的用途。
项24:用于相对于对照植物增强植物中产量相关性状的方法,其包括调节植物中编码底物蛋白-样多肽的核酸表达,其中所述底物蛋白-样多肽包含ENTH结构域。
项25:根据项24的方法,其中所述底物蛋白-样多肽包含两个或多个以下基序:
(i)基序1:(V/I)(L/R)(D/E)AT(S/D/N)(N/D/E/S)E(P/S)WGPHG(T/S/E)(SEQ IDNO:48),
(ii)基序2:F(Q/E)(Y/F)(I/L/V/R/K)(D/E)(S/P/A)(S/G/N/Q/R)G(R/K)D(Q/V/A/H/E)G(S/N/L/I/V)NVR(SEQ ID NO:49),
(iii)基序3:(E/S/A/Q)(V/I/E/A)R(Q/E/D/N)KA(A/L/V/E)(A/V/S/R/K)(N/T)(R/A)(D/E/N/G)K(SEQ ID NO:50),
(iv)基序4:WAD(T/S)LSRGL(V/I)(SEQ ID NO:51)
(v)基序5:L(A/S)D(I/V)G(I/V)(D/V)(F/G)(D/E/P/G)(SEQ ID NO:52)
项26:根据项24或25的方法,其中所述调节的表达通过在植物中引入并表达编码底物蛋白-样多肽的核酸实现。
项27:根据前述项24至26中任意项的方法,其中所述的编码底物蛋白-样多肽的核酸编码表A中所列的任一种蛋白质或是这种核酸的一部分或是能够与这种核酸杂交的核酸。
项28:根据前述项24至27中任意项的方法,其中所述的核酸序列编码表A中给出的任意蛋白质的直向同源物或旁系同源物。
项29:根据前述项24至28中任意项的方法,其中所述的增强的产量相关性状包括相对于对照植物提高的产量、优选提高的生物量和/或提高的种子产量。
项30:根据项24至29中任一项的方法,其中所述增强的产量相关性状在非胁迫条件下获得。
项31:根据项24至29中任一项的方法,其中所述增强的产量相关性状在轻度干旱条件下获得。
项32:根据项26至31中任一项的方法,其中所述核酸有效连接至组成型启动子,优选至GOS2启动子,最优选至来自稻的GOS2启动子。
项33:根据前述项24至32中任意项的方法,其中所述编码底物蛋白-样多肽的核酸是植物来源的,优选地来自双子叶植物,进一步优选地来自十字花科,更优选地来自拟南芥属,最优选地来自拟南芥。
项34:由根据前述项24至33中任意项的方法可获得的植物或其部分,包括种子,其中所述植物或其部分包含编码底物蛋白-样多肽的重组核酸。
项35:构建体,其包含:
(i)编码如项24或25定义的底物蛋白-样多肽的核酸;
(ii)能够驱动(a)的核酸序列表达的一个或多个调控序列;和任选地
(iii)转录终止序列。
项36:根据项35的构建体,其中所述调控序列之一是组成型启动子,优选是GOS2启动子,最优选是来自稻的GOS2启动子。
项37:根据项35或36的构建体在用于制备植物的方法中的用途,所述植物相对于对照植物具有提高的产量,特别是提高的种子产量。
项38:用根据项35或36的构建体转化的植物、植物部分或植物细胞。
项39:用于制备转基因植物的方法,所述转基因植物相对于对照植物具有提高的产量、特别是提高的生物量和/或提高的种子产量,该方法包括:
(i)在植物中引入并表达编码如本文项目24或25定义的底物蛋白-样多肽的核酸;和
(ii)在促进植物生长和发育的条件下培育植物细胞。
项40:转基因植物,其相对于对照植物具有因编码如本文项目24或25定义的底物蛋白-样多肽的核酸的受调节表达引起的提高的产量、特别是提高的生物量和/或提高的种子产量,或从所述转基因植物衍生的转基因植物细胞。
项41:根据项34、38或40的转基因植物,或从其中衍生的转基因植物细胞,其中所述植物是作物植物或单子叶植物或谷物植物,如稻、玉米、小麦、大麦、粟、黑麦、小黑麦、高粱和燕麦。
项42:根据项41的植物的可收获部分,其中所述可收获部分优选是种子。
项43:产物,其衍生自根据项41的植物和/或从根据项42的植物的可收获部分。
项44:编码底物蛋白-样多肽的核酸在植物中相对于对照植物提高产量、特别是提高种子产量中的用途。
项45:包含选自以下的核酸分子的分离核酸分子:
a)编码SEQ ID NO:112、SEQ ID NO:138和SEQ ID NO:142中所示的多肽的核酸分子;
b)SEQ ID NO:111、SEQ ID NO:137和SEQ ID NO:141中所示的核酸分子;
c)核酸分子,其作为遗传密码简并性的结果可衍生自SEQ ID NO:112、SEQ ID NO:138或SEQ ID NO:142所示的多肽序列且赋予植物相对于对照植物增强的产量相关性状;
d)核酸分子,与SEQ ID NO:111、SEQ ID NO:137和SEQ IDNO:141中给出的氨基酸序列中任一个,以增加的优选顺序具有至少25%、26%、27%、28%、29%、30%、31%、32%、33%、34%、35%、36%、37%、38%、39%、40%、41%、42%、43%、44%、45%、46%、47%、48%、49%、50%、51%、52%、53%、54%、55%、56%、57%、58%、59%、60%、61%、62%、63%、64%、65%、66%、67%、68%、69%、70%、71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%、或99%或更高序列同一性且赋予植物相对于对照植物增强的产量相关性状;
e)核酸分子,其编码下述多肽,所述多肽与由(a)至(c)的核酸分子编码的多肽的氨基酸序列,以增加的优选顺序具有至少25%、26%、27%、28%、29%、30%、31%、32%、33%、34%、35%、36%、37%、38%、39%、40%、41%、42%、43%、44%、45%、46%、47%、48%、49%、50%、51%、52%、53%、54%、55%、56%、57%、58%、59%、60%、61%、62%、63%、64%、65%、66%、67%、68%、69%、70%、71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%、或99%或更高序列同一性且赋予植物相对于对照植物增强的产量相关性状;
f)能够在严格杂交条件下与(a)至(c)的核酸分子杂交核酸分子且赋予植物相对于对照植物增强的产量相关性状;
g)借助于针对多肽的单克隆或多克隆抗体分离的多肽的编码核酸分子,所述单克隆或多克隆抗体针对的多肽是通过(a)到(e)的核酸分子之一编码;
h)编码多肽的核酸分子,所述多肽包括共有序列或一个或多个多肽基序,所述基序如基序1(与SEQ ID NO:6对应)、基序2(与SEQ IDNO:7对应)、基序3(与SEQ ID NO:8对应)、基序4(与SEQ ID NO:9对应)或基序5(与SEQ ID NO:10对应);
i)包含多核苷酸的核酸分子,所述多核苷酸通过使用在SEQ IDNO:46(prm09481)和SEQ ID NO:47(prm09482)中所示的引物,扩增cDNA文库或者基因组文库来得到;和
j)用包含(a)或(b)的核酸分子的互补序列的探针或者用至少15nt、优选20nt、30nt、50nt、100nt、200nt或500nt的这样的核酸分子在严格条件下筛选适当的核酸文库得到的核酸分子,所述这样的核酸与在(a)到(e)中表征的核酸分子序列互补。
项46:由根据项45的核酸分子编码的多肽。
项47:相对于对照植物增强产量相关性状的方法,所述方法包括增加编码tRNAδ(2)-异戊烯焦磷酸转移酶(IPPT)多肽的核酸序列在植物种子中表达,所述IPPT多肽包括(i)具有InterPro登录号IPR002627的tRNA异戊烯基转移酶结构域;和(ii)N端ATP/GTP结合位点基序A(P环),和任选地选择具有增强的产量相关性状的植物。
项48:根据项47的方法,其中所述IPPT多肽(i)以增加的优选顺序与SEQ ID NO:199所示的N端ATP/GTP结合位点基序A(P环)具有至少70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性;和以增加的优选顺序与以下一个或多个基序具有至少70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性:(ii)SEQ ID NO:200所示的保守基序ⅠDSR(Q/L)(V/L/I);或(ii)SEQ ID NO:201所示的保守基序Ⅱ(N/D/S/T)(I/V)GTAKP(T/S);或(ii)SEQ ID NO:202所示的保守基序ⅢL(V/A/I)GG(S/T)GLY;或(iv)SEQ ID NO:203所示的保守基序ⅣF/Y/L)AK(R/K/Q)Q(R/K/M)TWFR。
项49:根据项47或48的方法,其中所述IPPT多肽以增加的优选顺序SEQ ID NO:144所示的IPPT多肽或本文表A中给出的任多肽序列具有至少35%、40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、95%、98%、99%或更高氨基酸序列同一性。
项50:根据前述项47至49中任意项的方法,其中所述IPPT多肽能互补缺乏内源IPPT活性的酵母mod5突变株,或能互补缺乏内源IPPT活性的大肠杆菌miaA突变株。
项51:根据前述项47至50中任意项的方法,其中所述编码IPPT多肽的核酸序列由表A中给出的任一核酸序列SEQ ID NO或其部分或能与表A中给出的任一核酸序列SEQ ID NO杂交的序列代表。
项52:根据前述项47至51中任意项的方法,其中所述核酸序列编码表A中所列任意多肽序列SEQ ID NO的直向同源物或旁系同源物。
项53:根据前述项47至52中任意项的方法,其中所述增加的表达通过T-DNA活化标签技术、TILLING或同源重组中任一种或多种实现。
项54:根据前述项47至53中任意项的方法,其中所述增强的表达通过在植物种子中引入并表达编码IPPT多肽的核酸序列实现。
项55:根据前述项47至54中任意项的方法,其中所述增加的产量相关性状是提高的早期萌发势、提高的地上部分生物量、提高的每株植物种子总产量、提高的种子总数、提高的饱满种子数、提高的每穗花数和提高的收获指数中一项或多项。
项56:根据前述项47至55中任意项的方法,其中所述核酸序列有效连接至种子特异性启动子。
项57:根据项56的方法,其中所述种子特异性启动子是脱水蛋白启动子,优选稻脱水蛋白启动子,更优选SEQ ID NO:204所示的脱水蛋白启动子。
项58:根据项56的方法,其中所述种子特异性启动子是蛋白酶抑制剂启动子,优选稻蛋白酶抑制剂启动子,更优选SEQ ID NO:205所示蛋白酶抑制剂启动子。
项56:根据前述项47至58中任意项的方法,其中所述编码IPPT多肽的核酸序列来自原核生物界,优选来自蓝藻,进一步优选来自色球藻目,更优选来自聚球藻属物种,最优选来自聚球藻PCC 7942。
项60:由根据前述项47至59中任一项的方法可获得的植物、其部分(包括种子)或植物细胞,其中所述植物、其部分或细胞包含有效连接至种子特异性启动子的编码IPPT多肽的分离的核酸转基因。
项61:构建体,其包含:
(a)编码如项47至52中任一项中定义的IPPT多肽的核酸序列;
(b)能够驱动(a)的核酸序列表达的一个或多个调控序列;和任选地
(c)转录终止序列。
项62:根据项61的构建体,其中所述调控序列是种子特异性启动子。
项63:根据项62的构建体,其中所述种子特异性启动子是脱水蛋白启动子,优选稻脱水蛋白启动子,更优选SEQ ID NO:204所示的脱水蛋白启动子。
项64:根据项62的构建体,其中所述种子特异性启动子是蛋白酶抑制剂启动子,优选稻蛋白酶抑制剂启动子,更优选SEQ ID NO:205所示蛋白酶抑制剂启动子。
项65:根据项61至64中任一项的构建体在用于制备植物的方法中的用途,所述植物相对于对照植物具有增强的产量相关性状,所述增强的产量相关性状是提高的早期萌发势、提高的地上部分生物量、提高的每株植物种子总产量、提高的种子总数、提高的饱满种子数、提高的每穗花数、和提高的收获指数中一项或多项。
项66:用根据项61至64中任一项的构建体转化的植物、植物部分或植物细胞。
项67:用于制备转基因植物的方法,所述转基因植物相对于对照植物具有增强的产量相关性状,该方法包括:
(i)在种子特异性启动子的调控下,在植物、植物部分或植物细胞中引入并表达编码如项47至52中任一项定义的IPPT多肽的核酸序列;和
(ii)在促进植物生长和发育的条件下培育植物细胞、植物部分或植物。
项68:转基因植物,其相对于对照植物在种子中具有因编码如本文项47至52中任一项定义的与种子特异性启动子有效连接的IPPT多肽的核酸序列的增加的表达引起的增强的产量相关性状,或从所述转基因植物衍生的转基因植物细胞或转基因植物部分。
项69:根据项60、66或68的转基因植物,或从所述转基因植物中衍生的转基因植物细胞,其中所述植物是作物植物或单子叶植物或谷物植物,如稻、玉米、小麦、大麦、粟、黑麦、小黑麦、高粱和燕麦。
项70:根据项69的植物的可收获部分包括编码植物的IPPT多肽的分离核酸序列,其中所述可收获部分优选是种子。
项71:产物,其衍生自根据项69的植物和/或从根据项70的植物的可收获部分。
项72:编码如项45至50中任一项定义的IPPT多肽的核酸序列在提高产量相关性状中的用途,所述增强的产量相关性状包括提高的早期萌发势、提高的地上部分生物量、提高的每株植物种子总产量、提高的种子总数、提高的饱满种子数、提高的每穗花数、和提高的收获指数中一项或多项。
项73:用于相对于对照植物增强产量相关性状的方法,其包括调节编码SHR多肽的核酸在亚最佳养分可利用性条件下培育的植物中表达。
项74:用于相对于对照植物提高千粒重(TKW)的方法,其包括调节编码SHR多肽的核酸在无养分限制条件下培育的植物中表达。
项75:根据项73或74的方法,其中所述SHR多肽包含任意下述全长多肽,当其用于构建诸如图14所示的GRAS系统树时,与包含SEQ ID NO:209所示的氨基酸序列的SHR多肽组聚簇,而不与任何其他组聚簇。
项76:根据项73或75的方法,其中所述亚最佳养分可利用性条件是相对于对照植物降低的氮可利用性。
项77:根据前述项73至76中任意项的方法,其中所述调节的表达通过在植物中引入和表达编码SHR多肽的核酸实现。
项78:根据前述项73至77中任意项的方法,其中所述编码SHR多肽的核酸编码表A中所列的任一种蛋白质或是这种核酸的一部分或是能够与这种核酸杂交的核酸。
项79:根据前述项73至78中任意项的方法,其中所述核酸序列编码表A中给出的任意蛋白质的直向同源物或旁系同源物。
项80:根据项73或75至79中任一项的方法,其中所述增强的产量相关性状包括相对于对照植物提高的产量、优选提高的生物量和/或提高的种子产量。
项81:根据项77至80中任一项的方法,其中所述核酸有效链接至组成型启动子,优选至GOS2启动子,最优选至来自稻的GOS2启动子。
项82:根据项73至81中任一项的方法,其中所述编码SHR多肽的核酸优选是植物来源的,优选地来自双子叶植物,进一步优选地来自十字花科,更优选地来自拟南芥属,最优选地来自拟南芥。
项83:由根据前述项73至82中任意项的方法可获得的植物、其部分(包括种子),其中所述植物、或其部分包含编码SHR多肽的重组核酸。
项84:构建体,其包含:
(i)编码如项75定义的SHR多肽的核酸;
(ii)能够驱动(i)的核酸序列表达的一个或多个调控序列;和任选地
(iii)转录终止序列。
项85:根据项84的构建体,其中所述调控序列之一是组成型启动子,优选GOS2启动子,最优选来自稻的GOS2启动子。
项86:根据项84或85的构建体在用于制备植物的方法中的用途,所述植物相对于对照植物具有提高的产量,尤其提高的生物量和/或提高的种子产量。
项87:根据项84或85的构建体在用于制备具有提高的TKW的植物的方法中的用途。
项88:用根据项84或85的构建体转化的植物、植物部分或植物细胞。
项89:用于制备相对于对照植物具有增强的产量相关性状的转基因植物的方法,其包括:
(i)在植物中引入并表达编码如项75定义的SHR的核酸;和
(ii)在降低的营养可获得性的条件下培育所述植物细胞。
项90:用于制备相对于对照植物具有提高的TKW的转基因植物的方法,其包括:
(i)在植物中引入并表达编码如项75定义的SHR的核酸;和
(ii)在无养分限制条件下培育所述植物细胞。
项91:产物,其衍生自根据项83或88的植物和/或从根据项83或88的植物的可收获部分。
项92:编码SHR多肽的核酸在相对于对照植物增强植物的产量相关性状,尤其提高植物的种子产量和/或枝条生物量中的用途。
项93:编码SHR多肽的核酸在相对于对照植物提高植物TKW中的用途。
实施例
本发明现在参考如下实施例进行描述,所述实施例仅是示意性的。以下实施例不意图完全限定或限制本发明的范围。
DNA操作:除非另外说明,重组DNA技术根据(Sambrook(2001)Molecular Cloning:a laboratory manual,第3版Cold Spring HarborLaboratory Press,C SH,New York)或Ausubel等,(1994),CurrentProtocols in Molecular Biology,Current Protocols第1卷和第2卷中描述的标准方案进行。用于植物分子研究工作的标准材料和方法在BIOS科学出版有限责任公司(BIOS Scientific Publications Ltd(英国))和Blackwell科学出版社(Blackwell Scientific Publications(英国))出版的R.D.D.Croy的Plant Molecular Biology Labfax(1993)中描述。
实施例1:鉴定与本发明方法中所用核酸序列相关的序列
使用数据库序列搜索工具,如基本局部比对工具(BLAST)(Altschul等(1990)J.Mol.Biol.215:403-410;和Altschul等(1997)Nucleic Acids Res.25:3389-3402)在国家生物技术信息中心(NCBI)的Entrez核苷酸数据库中所维护的那些序列内鉴定到与本发明方法中所用核酸序列相关的(全长cDNA、EST或基因组)序列。该程序用来通过核酸序列或多肽序列与序列数据库比较并通过计算匹配的统计学显著性而找到序列间具有局部相似性的区域。例如,本发明所用核酸编码的多肽使用TBLASTN算法,采用默认设置和过滤以忽略低复杂性序列抵消。分析的结果通过配对性比较显示,并根据几率评分(E-值)排序,其中该评分反映特定比对结果因偶然而发生的概率(E-值越低,命中的显著性越高)。除了E-值外,比较还通过同一性百分数进行记分。同一性百分数指两个所比较核酸(或多肽)序列之间在特定长度范围内的相同核苷酸(或氨基酸)数目。在某些情况下,可以调整默认参数以改进研究的严格性。例如,可以提高E-值以表示较低严格性匹配。这样,可以识别出不足的几乎正确的匹配。
列表A1提供了与SEQ ID NO:1相关的核酸序列,列表A2提供了与SEQ ID NO:3相关的核酸序列。
如本文所用的表述“列表A1”与“表A1”相当且可互换。
如本文所用的表述“列表A2”与“表A2”相当且可互换。
在本说明书中使用的术语“表A”将用来说明表A1、表A2、表A3、表A4,和/或A5的内容。
在本说明书中使用的术语“表A1”将用来说明表A1的内容。在本说明书中使用的术语“表A2”将用来说明表A2的内容。在本说明书中使用的术语“表A3”将用来说明表A3的内容。在本说明书中使用的术语“表A4”将用来说明表A4的内容。在本说明书中使用的术语“表A5”将用来说明表A5的内容。在一个优选的实施方案中,术语“表A”意指表A1。在另一个优选的实施方案中,术语“表A”意指表A2。在另一个优选的实施方案中,术语“表A”意指表A3。在另一个优选的实施方案中,术语“表A”意指表A4。在另一个优选的实施方案中,术语“表A”意指表A5。
在本说明书中使用的术语“表B”将用来说明表B1、表B2、表B3,和/或表B4的内容。
在本说明书中使用的术语“表B1”将用来说明表B1的内容。在本说明书中使用的术语“表B2”将用来说明表B2的内容。在本说明书中使用的术语“表B3”将用来说明表B3的内容。在本说明书中使用的术语“表B4”将用来说明表B4的内容。在本说明书中使用的术语“表B5”将用来说明表B5的内容。在一个优选的实施方案中,术语“表B”意指表B1。在另一个优选的实施方案中,术语“表B”意指表B2。在另一个优选的实施方案中,术语“表B”意指表B3。在另一个优选的实施方案中,术语“表B”意指表B4。
表A1:SEQ ID NO:1相关的序列
表A2.SEQ ID NO:3相关的序列
名称 | 植物来源 | 核酸SEQ ID NO: | 蛋白质SEQ ID NO: |
Mt_TCP2_sugar | 蒺藜苜蓿 | 3 | 4 |
Am\TCP\CAE45599 | 金鱼草 | 19 | 20 |
AT3G47620 | 拟南芥 | 21 | 22 |
AtTCP15 | 拟南芥 | 23 | 24 |
Gh\TCP\AAD48836 | 棉花 | 25 | 26 |
OSTCP12 | 稻 | 27 | 28 |
OsTCP5 | 稻 | 29 | 30 |
Pt\TCP\scaff_124.66\[1298]\f\[31-1218] | 毛果杨 | 31 | 32 |
Sd\TCP\AAT38718 | 马铃薯 | 33 | 34 |
Vv\TCP\AAD48836 | 葡萄 | 35 | 36 |
Vv\TCP\CAO62540 | 葡萄 | 37 | 38 |
对于底物蛋白-样序列,表A3提供本发明方法中使用的核酸序列相关的核酸序列的列表。
表A3:底物蛋白-样多肽的实例:
植物来源 | 核酸SEQ ID NO: | 蛋白质SEQ ID NO: |
拟南芥 | 43 | 44 |
拟南芥 | 65 | 66 |
葡萄 | 67 | 68 |
稻 | 69 | 70 |
稻 | 71 | 72 |
野燕麦 | 73 | 74 |
蒺藜苜蓿 | 75 | 76 |
拟南芥 | 77 | 78 |
拟南芥 | 79 | 80 |
拟南芥 | 81 | 82 |
拟南芥 | 83 | 84 |
稻 | 85 | 86 |
拟南芥 | 87 | 88 |
葡萄 | 89 | 90 |
拟南芥 | 91 | 92 |
拟南芥 | 93 | 94 |
葡萄 | 95 | 96 |
莱菌衣藻 | 97 | 98 |
Ostreococcuslucimarinus | 99 | 100 |
稻 | 101 | 102 |
稻 | 103 | |
稻 | 104 | |
稻 | 105 | 106 |
稻 | 107 | |
稻 | 108 | 109 |
稻 | 110 | |
欧洲油菜 | 111 | 112 |
大豆 | 113 | 114 |
大麦 | 115 | 116 |
蒺藜苜蓿 | 117 | 118 |
蒺藜苜蓿 | 119 | 120 |
展叶剑叶藓 | 121 | 122 |
展叶剑叶藓 | 123 | 124 |
展叶剑叶藓 | 125 | 126 |
毛果杨 | 127 | 128 |
毛果杨 | 129 | 130 |
毛果杨 | 131 | 132 |
番茄 | 133 | 134 |
普通小麦 | 135 | 136 |
普通小麦 | 137 | 138 |
拟南芥 | 139 | 140 |
玉蜀黍 | 141 | 142 |
在一些情况下,相关序列已经由研究机构如基因组研究机构(TheInstitute for Genomic Research,TIGR)初步地汇编并且公开披露。可以使用真核生物基因直向同源物(Eukaryotic Gene Orthologs,EGO)数据库来鉴定此类相关序列,这可通过关键词搜索或通过使用BLAST算法以目的核酸或多肽序列进行。
对于IPPT,表A4提供本发明方法中使用的核酸序列相关的核酸序列的列表。
表A4:IPPT多肽序列和编码核酸序列的实例:
名称 | 来源生物 | 公开数据库登录号 | 核酸序列SEQID NO: | 多肽序列SEQIDNO: |
Synec_IPPT | 聚球藻属物种PCC 7942 | U30252.3 | 143 | 144 |
Acama_IPPT(miaA) | Acaryochloris marinaMBIC11017 | CP000828 | 145 | 146 |
Anava_IPPT | 多变鱼腥藻ATCC 29413 | CP000117 | 147 | 148 |
Glovi_IPPT | 无类囊体蓝藻PCC 7421 | BA000045 | 149 | 150 |
Micae_IPPT | Microcystis aeruginosa PCC7806 | AM778958 | 151 | 152 |
Nossp_IPPT | 念球藻属物种PCC 7120DNA | BA000019 | 153 | 154 |
Proma1375_IPPT | 海洋原绿球藻subsp.marinusstr.CCMP1375 | AE017126 | 155 | 156 |
Proma9211_IPPT | 海洋原绿球藻str.MIT 9211 | CP000878 | 157 | 158 |
Proma9215_IPPT | 海洋原绿球藻str.MIT 9215 | CP000825 | 159 | 160 |
Proma9301_IPPT | 海洋原绿球藻str.MIT 9301 | CP000576 | 161 | 162 |
Proma9303_IPPT | 海洋原绿球藻str.MIT 9303 | CP000554 | 163 | 164 |
Proma9312_IPPT | 海洋原绿球藻str.MIT 9312 | CP000111 | 165 | 166 |
Proma9313_IPPT | 海洋原绿球藻MIT9313 | BX572095 | 167 | 168 |
Proma9515_IPPT | 海洋原绿球藻str.MIT 9515 | CP000552 | 169 | 170 |
Proma9601_IPPT | 海洋原绿球藻str.AS9601 | CP000551 | 171 | 172 |
PromaMED4_IPPT | 海洋原绿球藻MED4 | BX548174 | 173 | 174 |
PromaNATL1A_IPPT | 海洋原绿球藻str.NATL1A | CP000553 | 175 | 176 |
PromaNATL2A_IPPT | 海洋原绿球藻str.NATL2A | CP000095 | 177 | 178 |
SynecJA-3_IPPT | 聚球藻属物种JA-3-3Ab | CP000239 | 179 | 180 |
Synec307_IPPT | 聚球藻属物种RCC307 | CT978603 | 181 | 182 |
Synec6803_IPPT | 集胞藻属物种PCC 6803DNA | BA000022 | 183 | 184 |
Synec7803_IPPT | Synechococcus WH7803 | CT971583 | 185 | 186 |
Synec8102_IPPT | 聚球藻属物种WH8102 | BX569689.1 | 187 | 188 |
Synec9311_IPPT | 聚球藻属物种CC9311 | CP000435 | 189 | 190 |
Synec9605_IPPT | 聚球藻属物种CC9605 | CP000110 | 191 | 192 |
Synec9902_IPPT | 聚球藻属物种CC9902 | CP000097 | 193 | 194 |
Theel_IPPT | 嗜热蓝细菌聚球藻BP-1 | BA000039 | 195 | 196 |
Trier_IPPT | 红海束毛藻IMS101 | CP000393 | 197 | 198 |
在一些情况下,相关序列已经由研究机构如基因组研究机构(TheInstitute for Genomic Research,TIGR)初步地汇编并且公开披露。可以使用真核生物基因直向同源物(Eukaryotic Gene Orthologs,EGO)数据库来鉴定此类相关序列,这可通过关键词搜索或通过使用BLAST算法以目的核酸或多肽序列进行。对于其他情况,为了特定生物创建了专用核酸序列数据库,例如联合基因组研究所(Joint GenomeInstitute)所作的。
对于SHR,表A5提供本发明方法中使用的核酸序列相关的核酸序列的列表。
表A5:SHR多肽的实例
名称 | 来源生物 | 核酸SEQ ID NO: | 多肽SEQ IDNO: |
拟南芥 | 208 | 209 | |
At4g37650 | 拟南芥 | 210 | 211 |
TA13018_3352 | 火炬松 | 212 | 213 |
22633_part | 展叶剑叶藓 | 214 | 215 |
14911_part | 展叶剑叶藓 | 216 | 217 |
Os03g31880 | 稻 | 218 | 219 |
Os07g39820 | 稻 | 220 | 221 |
US200510879.113 | 玉蜀黍 | 222 | 223 |
TA7750_4236 | 莴苣 | 224 | 225 |
AC147000 | 蒺藜苜蓿 | 226 | 227 |
TC153082 | 马铃薯 | 228 | 229 |
WO2005001020_215 | 巨桉 | 230 | 231 |
AM431974 | 葡萄 | 232 | 233 |
scaff_186.17 | 毛果杨 | 234 | 235 |
TA2955_3988 | 蓖麻 | 236 | 237 |
US2004031072.68433 | 大豆 | 238 | 239 |
CT027662 | 蒺藜苜蓿 | 240 | 241 |
实施例2:TCP多肽序列的比对
使用来自Vector NTI(Invitrogen)的AlignX程序进行多肽序列的比对,其中所述AlignX程序基于流行的累进比对Clustal W算法(Thompson等(1997)Nucleic Acids Res 25:4876-4882;Chenna等(2003),Nucleic Acids Res 31:3497-3500)。空位开口罚分的默认值是10,空位延伸罚分是0.1并且所选的权重矩阵是Blosum 62(若比对多肽)。进行少许手工编辑以优化该比对。TCP1多肽在图1中比对且TCP2多肽在图2中比对。
使用如Vector NTI(Invitrogen)的AlignX程序中所提供的邻接法聚簇算法构建TCP多肽的系统树(图1和2)。
对于底物蛋白-样序列,默认空位开口罚分的默认值是10,空位延伸罚分是0.1并且所选的权重矩阵是Gonnet(若比对多肽)。进行少许手工编辑以优化该比对。底物蛋白-样多肽之间的序列保守性基本上在所述多肽的N端ENTH结构域和C端结构域部分,中间部分通常在序列长度和组成上更加可变。底物蛋白-样多肽在图2中比对。
使用(来自Invitrogen公司Vector NTI 10.3的)AlignX算法进行表A4中全部IPPT多肽序列的多重序列比对。在本申请的图3中显示比对结果。用框标出SEQ ID NO:199所示的N端ATP/GTP结合位点基序A(P-环)、SEQ ID NO:200所示的保守基序ⅠDSR(Q/L)(V/L/I)、SEQ ID NO:201所示的保守基序Ⅱ(N/D/S/T)(I/V)GTAKP(T/S)、SEQID NO:202所示的保守基序ⅢL(V/A/I)GG(S/T)GLY、和保守基序ⅣF/Y/L)AK(R/K/Q)Q(R/K/M)TWFR。用括号标出在真核tRNA-IPT中发现的推定锌指基序C2H2(C-X2-C-X(12,18)-H-X5-H,并用框标出保守Cys和His残基。
对于SHR,使用来自Vector NTI(Invitrogen)的AlignX程序进行多肽序列的比对,其中所述AlignX程序基于流行的累进比对Clustal W算法(Thompson等(1997)Nucleic Acids Res 25:4876-4882;Chenna等(2003),Nucleic Acids Res 31:3497-3500)。空位开口罚分的默认值是10,空位延伸罚分是0.1并且所选的权重矩阵是Blosum 62(若比对多肽)。进行少许手工编辑以优化该比对。
对于SHR序列,构建GRAS多肽的系统树(图14)。使用来自稻、拟南芥的GRAS蛋白质和来自各种组织的SHR相关蛋白质构建GRAS和SHR蛋白质的邻接树,使用MUSCLE比对。用CLUSTALX产生邻接树。进行100次重复bootstrap分析。显示主要节点的bootstrap支持值。指出SHR相关蛋白质。A.thaliana:拟南芥;E.grandis:巨桉;G.max:大豆;L.sativa:生菜;M trucatula:蒺藜苜蓿;O.sativa:稻;P.taeda:火炬松;P.patens:展叶剑叶藓;P.trichocarpa:毛果杨;R.communis:蓖麻;S.tuberosum:马铃薯;V.vinifera:葡萄;Z.mays:玉蜀黍;-part:部分序列。
实施例3:计算在实施本发明方法中有用的多肽序列之间的全局同一性百分数
对于TCP1或TCP2,使用现有技术领域可获得的方法之一,即MatGAT(矩阵总体比对工具)软件(BMC Bioinformatics.20034:29.MatGAT:使用蛋白质序列或DNA序列产生相似性/同一性矩阵的一项应用(an application that基因rates similarity/identity matrices usingprotein或DNA序列s),Campanella JJ,Bitincka L,Smalley J;该软件由Ledion Bitincka维护)确定在实施本发明方法中有用的全长多肽序列之间的全局相似性和同一性百分数。MatGAT软件对DNA序列或蛋白质序列产生相似性/同一性矩阵,无需预先比对数据。该程序使用Myers和Miller全局比对算法(空位开口罚分12和空位延伸罚分2)执行一系列配对比对,使用例如Blosum 62(对于多肽而言)计算相似性和同一性并且随后将结果置于距离矩阵中。在分割线下半部分显示序列相似性,并且在对角分割线的上半部分显示序列同一性。
比较中使用的参数是:
评分矩阵:Blosum62
第一空位:12
延伸空位:2
表B中显示在多肽序列全长范围内的全局相似性和同一性的软件分析结果。在对角线下方给出相似性百分数(粗体)而在对角线上方给出同一性百分数(正常字体)。
表B:在该多肽序列的全长范围内全局相似性和同一性的MatGAT结果。
表B1:Ms_TCP_SUGAR家族(TCP1)
表B2:Mt_TCP2_SUGAR家族(TCP2)
还可以产生针对特定结构域的局部比对的MATGAT表或特定结构域之间的同一性/相似性百分比的数据。
对于底物蛋白-样序列,使用现有技术领域可获得的方法之一,即MatGAT(矩阵总体比对工具)软件(BMC Bioinformatics.20034:29.MatGAT:使用蛋白质序列或DNA序列产生相似性/同一性矩阵的一项应用(an application that基因rates similarity/identity matrices usingprotein或DNA序列s),Campanella JJ,Bitincka L,Smalley J;该软件由Ledion Bitincka维护)确定在实施本发明方法中有用的全长多肽序列之间的全局相似性和同一性百分数。MatGAT软件对DNA序列或蛋白质序列产生相似性/同一性矩阵,无需预先比对数据。该程序使用Myers和Miller全局比对算法(空位开口罚分12和空位延伸罚分2)执行一系列配对比对,使用例如Blosum 62(对于多肽而言)计算相似性和同一性并且随后将结果置于距离矩阵中。在分割线下半部分显示序列相似性,并且在对角分割线的上半部分显示序列同一性。
对于底物蛋白-样蛋白质,比较中使用的参数是:
评分矩阵:Blosum62
第一空位:12
延伸空位:2
表B3中显示在多肽序列的全长范围内全局相似性和同一性的软件分析结果。在对角线上方给出同一性百分数(粗体)而在对角线下方给出相似性百分数(正常字体)。
与SEQ ID NO:44相比,在实施本发明方法中有用的底物蛋白-样蛋白质序列之间的同一性百分比可低至14%氨基酸同一性。
表B3:该多肽序列全长范围内全局相似性和同一性的MatGAT结果。
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | |
1.SEQID2 | 96.4 | 59.5 | 46.1 | 41.0 | 40.9 | 39.5 | 37.9 | 43.0 | 25.0 | 25.4 | 25.4 | 30.7 | |
2.CAB87689 | 96.5 | 58.2 | 45.1 | 39.9 | 40.1 | 38.4 | 37.2 | 42.0 | 25.7 | 25.5 | 25.5 | 30.9 | |
3.CAO43767 | 73.4 | 71.6 | 46.7 | 42.0 | 41.5 | 40.9 | 39.4 | 43.8 | 25.4 | 27.8 | 27.8 | 31.1 | |
4.CAD41810 | 61.3 | 61.3 | 61.8 | 44.3 | 67.3 | 42.4 | 40.2 | 95.8 | 26.6 | 25.7 | 25.7 | 28.8 | |
5.BAD87030 | 58.8 | 59.2 | 58.6 | 58.9 | 40.8 | 88.0 | 82.2 | 42.8 | 24.8 | 25.0 | 25.0 | 27.5 | |
6.AAB68030 | 56.1 | 56.1 | 55.3 | 76.0 | 57.5 | 40.5 | 39.3 | 64.3 | 26.8 | 26.9 | 26.9 | 29.3 | |
7.EAZ13473 | 57.5 | 56.3 | 58.3 | 58.1 | 91.2 | 56.4 | 91.1 | 40.8 | 23.9 | 23.8 | 23.8 | 27.2 | |
8.EAY75756 | 54.2 | 53.7 | 55.7 | 58.1 | 89.2 | 55.3 | 93.5 | 38.6 | 23.2 | 24.3 | 24.0 | 27.7 | |
9.EAY95411 | 58.5 | 58.5 | 59.4 | 96.3 | 56.5 | 73.2 | 55.6 | 55.7 | 25.2 | 24.2 | 24.2 | 26.8 | |
10.ABN08674 | 36.8 | 36.6 | 37.2 | 35.8 | 37.8 | 38.1 | 36.4 | 35.8 | 34.4 | 50.0 | 50.1 | 38.0 | |
11.BAF01674 | 39.1 | 38.4 | 40.7 | 38.1 | 38.9 | 38.5 | 37.3 | 38.0 | 36.6 | 62.7 | 99.9 | 72.1 | |
12.NP_850387 | 39.1 | 38.4 | 40.7 | 38.1 | 38.9 | 38.4 | 37.3 | 37.7 | 36.6 | 62.7 | 100.0 | 72.2 | |
13.BAD44158 | 42.3 | 43.7 | 45.8 | 43.5 | 47.2 | 43.8 | 46.9 | 44.4 | 41.5 | 45.9 | 72.2 | 72.2 | |
14.AAN72258 | 39.6 | 38.9 | 40.7 | 38.3 | 38.8 | 38.4 | 37.2 | 37.5 | 36.9 | 62.5 | 99.8 | 99.8 | 72.1 |
15.BAD19387 | 36.7 | 36.3 | 37.1 | 36.7 | 37.5 | 37.6 | 36.6 | 36.5 | 35.2 | 58.3 | 58.3 | 58.3 | 42.0 |
16.EAZ25008 | 36.7 | 36.3 | 37.1 | 36.7 | 37.5 | 37.6 | 36.6 | 36.5 | 35.2 | 58.3 | 58.3 | 58.3 | 42.0 |
17.CAB91599 | 33.3 | 33.6 | 34.6 | 34.6 | 34.5 | 35.2 | 34.5 | 34.1 | 33.5 | 59.2 | 70.5 | 70.5 | 50.9 |
18.CAO45312 | 37.4 | 38.2 | 39.1 | 38.3 | 39.7 | 40.5 | 38.6 | 38.9 | 36.8 | 58.3 | 63.6 | 63.6 | 47.8 |
19.AAL24360 | 33.5 | 33.6 | 34.9 | 34.8 | 34.5 | 35.2 | 34.5 | 34.0 | 33.7 | 59.1 | 70.4 | 70.4 | 50.9 |
20.AAC64305 | 34.1 | 33.4 | 35.3 | 31.6 | 30.7 | 28.2 | 31.7 | 30.7 | 29.5 | 24.9 | 29.5 | 29.5 | 41.3 |
21.CAN66991 | 33.8 | 34.0 | 36.7 | 35.9 | 35.6 | 35.4 | 33.8 | 34.8 | 35.0 | 56.3 | 58.7 | 58.7 | 49.7 |
22.XP001701452 | 38.9 | 39.7 | 38.1 | 36.9 | 37.1 | 35.4 | 38.9 | 38.7 | 35.2 | 29.4 | 29.1 | 29.1 | 38.9 |
23.XP001419857 | 20.5 | 20.3 | 20.5 | 18.7 | 18.5 | 18.2 | 19.3 | 18.8 | 17.5 | 12.4 | 13.5 | 13.5 | 18.9 |
14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 |
1.SEQID2 | 25.9 | 25.2 | 25.3 | 22.7 | 27.0 | 22.7 | 23.9 | 22.6 | 23.2 | 14.3 |
2.CAB87689 | 26.0 | 24.5 | 24.6 | 22.9 | 27.0 | 22.9 | 23.6 | 22.1 | 23.8 | 14.0 |
3.CAO43767 | 27.8 | 25.5 | 25.5 | 24.1 | 27.0 | 24.2 | 25.5 | 22.8 | 24.0 | 13.9 |
4.CAD41810 | 25.6 | 24.9 | 25.1 | 23.8 | 25.8 | 23.9 | 22.7 | 22.2 | 22.1 | 13.1 |
5.BAD87030 | 25.0 | 25.1 | 25.1 | 22.5 | 25.7 | 22.6 | 21.2 | 21.5 | 23.1 | 12.9 |
6.AAB68030 | 26.9 | 25.5 | 25.5 | 24.1 | 26.3 | 24.2 | 20.7 | 23.3 | 22.7 | 12.2 |
7.EAZ13473 | 23.8 | 23.5 | 23.5 | 23.2 | 25.1 | 23.3 | 21.9 | 20.3 | 23.9 | 13.6 |
8.EAY75756 | 24.0 | 24.0 | 24.0 | 22.9 | 25.0 | 22.9 | 20.8 | 20.3 | 22.3 | 13.3 |
9.EAY95411 | 24.1 | 23.5 | 23.8 | 22.4 | 24.4 | 22.4 | 20.5 | 21.0 | 20.8 | 11.8 |
10.ABN08674 | 49.9 | 43.5 | 43.5 | 47.7 | 47.6 | 47.3 | 21.3 | 44.5 | 19.1 | 8.1 |
11.BAF01674 | 99.7 | 46.1 | 46.0 | 62.3 | 50.1 | 62.2 | 29.3 | 43.8 | 20.1 | 8.7 |
12.NP_850387 | 99.8 | 46.0 | 45.9 | 62.4 | 50.2 | 62.3 | 29.3 | 43.9 | 20.1 | 8.7 |
13.BAD44158 | 72.1 | 33.6 | 33.6 | 44.6 | 38.7 | 44.6 | 41.0 | 35.9 | 24.8 | 12.2 |
14.AAN72258 | 45.7 | 45.6 | 62.2 | 50.2 | 62.1 | 29.2 | 43.8 | 20.1 | 8.7 | |
15.BAD19387 | 58.1 | 99.9 | 40.8 | 44.7 | 40.7 | 20.1 | 40.7 | 20.7 | 8.0 | |
16.EAZ25008 | 58.1 | 100.0 | 40.7 | 44.6 | 40.6 | 20.1 | 40.6 | 20.7 | 8.0 | |
17.CAB91599 | 70.3 | 53.9 | 53.9 | 43.3 | 99.9 | 21.8 | 38.4 | 17.9 | 8.2 | |
18.CAO45312 | 63.7 | 56.8 | 56.8 | 54.9 | 43.2 | 22.6 | 77.7 | 20.6 | 8.9 | |
19.AAL24360 | 70.2 | 53.8 | 53.8 | 99.9 | 54.5 | 21.8 | 38.3 | 17.9 | 8.3 | |
20.AAC64305 | 29.4 | 23.9 | 23.9 | 23.9 | 25.7 | 23.9 | 18.9 | 16.2 | 26.5 | |
21.CAN66991 | 58.7 | 52.9 | 52.9 | 50.6 | 81.8 | 50.6 | 23.7 | 19.5 | 8.7 | |
22.XP001701452 | 29.1 | 30.7 | 30.7 | 26.6 | 31.3 | 26.6 | 26.2 | 31.7 | 14.2 | |
23.XP001419857 | 13.5 | 12.4 | 12.4 | 11.6 | 13.5 | 11.6 | 43.1 | 12.9 | 20.3 |
对于IPPT,使用现有技术领域可获得的方法之一,即MatGAT(矩阵总体比对工具)软件(BMC Bioinformatics.20034:29.MatGAT:使用蛋白质序列或DNA序列产生相似性/同一性矩阵的一项应用(anapplication that基因rates similarity/identity matrices using protein或DNA序列s),Campanella JJ,Bitincka L,Smalley J;该软件由LedionBitincka维护)确定在实施本发明方法中有用的全长多肽序列之间的全局相似性和同一性百分数。MatGAT软件对DNA序列或蛋白质序列产生相似性/同一性矩阵,无需预先比对数据。该程序使用Myers和Miller全局比对算法(空位开口罚分12和空位延伸罚分2)执行一系列配对比对,使用例如Blosum 62(对于多肽而言)计算相似性和同一性并且随后将结果置于距离矩阵中。在分割线下半部分显示序列相似性,并且在对角分割线的上半部分显示序列同一性。
比较中使用的参数是:
评分矩阵:Blosum62
第一空位:12
延伸空位:2
表B4中显示在多肽序列的全长范围内全局相似性和同一性的软件分析结果(不包括局部多肽序列)。
与SEQ ID NO:144相比,在实施本发明方法中有用的全长多肽序列之间的同一性百分比可低至39%氨基酸同一性。
对于SHR,使用现有技术领域可获得的方法之一,即MatGAT(矩阵总体比对工具)软件(BMC Bioinformatics.20034:29.MatGAT:使用蛋白质序列或DNA序列产生相似性/同一性矩阵的一项应用(an application that基因rates similarity/identity matrices using protein或DNA序列s),Campanella JJ,Bitincka L,Smalley J;该软件由Ledion Bitincka维护)确定在实施本发明方法中有用的全长多肽序列之间的全局相似性和同一性百分数。MatGAT软件对DNA序列或蛋白质序列产生相似性/同一性矩阵,无需预先比对数据。该程序使用Myers和Miller全局比对算法(空位开口罚分12和空位延伸罚分2)执行一系列配对比对,使用例如Blosum 62(对于多肽而言)计算相似性和同一性并且随后将结果置于距离矩阵中。
比较中使用的参数是:
评分矩阵:Blosum62
第一空位:12
延伸空位:2
实施例4:鉴定在实施本发明方法中有用的多肽序列中所包含的结构域
蛋白质家族、结构域和位点的集成资源(InterPro)数据库是针对基于文本及基于序列的搜索法的常用特征标识数据库的集成界面。InterPro数据库合并了这些数据库,所述数据库使用不同的方法学及不同程度的有关充分表征的蛋白质的生物学信息以获得蛋白质特征标识(proteinsignatures)。合作数据库包括SWISS-PROT、PROSITE、TrEMBL、PRINTS、Panther、ProDom和Pfam、Smart和TIGRFAM。Pfam是覆盖众多常见蛋白质结构域和家族的序列多重比对结果和隐匿马尔科夫模型(HMM)的庞大集合。Pfam在英国Sanger研究所服务器上维护。Interpro由英国欧洲生物信息学研究所维护。
在表C1中显示SEQ ID NO:44所示的多肽序列的InterPro扫描结果。
表C1:SEQ ID NO:44所示的多肽序列的InterPro扫描结果(主要登录号)。
数据库 | 登录号 | 登录号名称 | SEQ ID NO 44的氨基酸坐标 |
InterPro | IPR001026 | 底物蛋白,N端 | |
HMMPfam | PF01417 | ENTH | 25-148 |
HMMSmart | SM00273 | ENTH | 26-152 |
ProfileScan | PS50942 | ENTH | 20-152 |
InterPro | IPR008943 | 肌醇磷脂-结合网格蛋白衔接头,N端 | |
superfamily | SSF48473 | PI_bind_N | 25-238 |
在表C2中显示SEQ ID NO:144所示的多肽序列的InterPro扫描结果。
表C2:SEQ ID NO:144所示的多肽序列的InterPro扫描结果
InterPro登录号和名称 | 集成的数据库名称 | 集成的数据库登录号 | 集成的数据库登录名称 |
IPR002627tRNA异戊烯基转移酶 | BlastProDom | PD004674 | MIAA_SYNP7_Q8GIT6; |
IPR002627tRNA异戊烯基转移酶 | HMMPfam | PF01715.6 | IPP转移酶 |
IPR002627tRNA异戊烯基转移酶 | HMMTigr | TIGR00174 | miaA:tRNA δ(2)-异戊烯焦磷酸 |
IPR011593异戊烯基转移酶-样 | BlastProDom | PD005388 | MIAA_SYNP7_Q8GIT6 |
IPR非-集成 | tmhmm | PTHR11088 | TRNA Δ(2)-异戊烯焦磷酸转移酶-相关 |
IPR非-集成 | 超家族 | SSF52540 | 包含核苷三磷酸水解酶的P-环 |
实施例5:在实施本发明方法中有用的多肽序列的拓扑结构预测
TargetP 1.1预测真核蛋白的亚细胞定位。基于任何氨基端前序列:叶绿体转运肽(cTP)、线粒体靶向肽(mTP)或分泌途径信号肽(SP)的预测存在性进行定位指派。作为最终预测基础的评分并不真正是概率,并且它们不是必需地加合成一体。然而,根据TargetP,具有最高评分的定位是最可能的,并且评分之间的关系(可靠性级别)可以指示该预测具有多大确定性。可靠性级别(RC)范围从1至5,其中1表示最可靠的预测。TargetP在丹麦技术大学(Technical University of Denmark)的服务器上维护。
对于预测含有氨基端前序列的序列而言,也可以预测潜在的切割位点。
选择许多参数,如生物组别(非植物或植物)、临界值集合(无、预定义的临界值集合或用户指定的临界值集合)和切割位点预测的计算(是或否)。
许多其他算法可以用来进行此类分析,包括:
●在丹麦技术大学服务器上维护的ChloroP 1.1;
●在澳大利亚布里斯班昆士兰大学生物科学研究所的服务器上维护的Protein Prowler亚细胞定位预测者1.2版;
●在加拿大阿伯特省埃德蒙顿市阿尔伯塔大学的服务器上维护的PENCE蛋白组分析专家PA-GOSUB 2.5;
●在丹麦技术大学服务器上维护的TMHMM。
对于SEQ ID NO:44,选择许多参数,如生物组别(非植物或植物)、临界值集合(无、预定义的临界值集合或用户指定的临界值集合)和切割位点预测的计算(是或否)。
在表D中呈现SEQ ID NO:44所示的多肽序列的TargetP 1.1分析的结果。选择“植物”生物组别,未定义临界值,并且对转运肽的预测长度提出要求。SEQ ID NO:44所示的多肽序列的亚细胞定位可以是细胞质或细胞核,没有预测到转运肽。
表D:SEQ ID NO:44所示的多肽序列的TargetP 1.1分析结果
长度(AA) | 560 |
叶绿体转运肽 | 0.105 |
线粒体转运肽 | 0.100 |
分泌途径信号肽 | 0.168 |
其他亚细胞靶向 | 0.872 |
预测的位置 | / |
可靠性级别 | 2 |
预测的转运肽长度 | / |
实施例6:
SEQ ID NO:2或SEQ ID NO:4所示的多肽序列是具有DNA结合活性的转录因子转录因子结合特定DNA序列的能力可以通过电泳迁移率变动分析(EMSA;也称为凝胶阻滞分析)测定,所述电泳迁移率变动分析是本领域众所周知的,并特定对TCP有报导,Kosugi & Ohashi(2002)PlantJ 30:337-348,和Li等,(2005)PNAS 102(36):12978-83。还被Kosugi &Ohashi报导为检测二聚化模式和特异性的方法,其例如使用酵母双杂交系统,而Li等描述了表征TCP结合的启动子的染色质免疫沉淀试验。
对于底物蛋白-样多肽,脂质结合可如Hom等(2007)所述实施。混合磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸(Avanti)和磷脂酰肌醇(4,5)二磷酸diC16(C 16-PtdIns(4,5)P2,Echelon Biosciences Inc.)溶于CHCl3/MeOH/H2O(65∶25∶4,体积)的溶液且真空干燥。脂质重悬于50mM Tris、100mM KCl(pH 7.0)且在64℃下温育1小时。然后在液氮中冷冻、在37℃下融化脂质体三个循环。脂质体溶液通过Avanti挤压机产生1.0μm脂质体。脂质体通过以25,000g离心10分钟收集,在100μl 20mM Tris、100mM KCl缓冲液(pH 6.0,7.0or 8.0)中重悬,得到终浓度为2mM的最终脂质体。脂质体与GST-融合体ENTH和ANTH结构域,或GST(最终蛋白质浓度2-5μg/ml)在室温下温育30分钟,然后再次离心收集。脂质体沉淀在100μl缓冲液中重悬,通过SDS-PAGE和考马斯亮蓝染色分析脂质结合蛋白的存在。
对于IPPT,用于实施本发明方法的多肽显示IPPT活性。存在许多可以测定此类IPPT活性的测定,包括具有缺陷型内源IPPT活性(由MOD5基因编码;Golovko等(2002)Plant Molec Biol 49:161-169)的酵母菌株的互补测定、具有缺陷型内源IPPT活性(由miaA基因编码;Dihanich等(1987)Mol Cell Biol 7:177-184)的大肠杆菌菌株的互补测定,或tRNA中细胞激动素的定量(Gray等(1996)Plant Physiol 110:431-438,Miyawaki等(2006)Proc Natl Acad SCi USA 103(44):16598-16603)。本领域技术人员很容易明白此类测定IPPT活性,包括SEQ ID NO:144所示的IPPT多肽的IPPT活性的实验步骤。
实施例7:在本发明方法中所用的核酸序列的克隆
TCP核酸序列的克隆
使用定制的苜蓿cDNA文库(在pCMV Sport 6.0中;Invitrogen,Paisley,UK)作为模板,通过PCR扩增在本发明方法中使用的核酸序列。使用Hifi Taq DNA聚合酶,在标准条件下使用50μl PCR混合物中的200ng模板进行PCR。使用的引物是:
TCP1-正义(SEQ ID NO:39):
5’GGGGACAAGTTTGTACAAAAAAGCAGGCTTAAACAATGTCTAACCACAAGGAAACA 3’
TCP1-反义,互补(SEQ ID NO:40):
5’GGGGACCACTTTGTACAAGAAAGCTGGGTGAATAAAGTACAAAACACCGAA 3’
TCP2-正义(SEQ ID NO:41):
5’GGGACAAGTTTGTACAAAAAAGCAGGCTTAAACAATGGAATTGGAAGGTGATCAT 3’
TCP2-反义,互补(SEQ ID NO:42):
5’GGGGACCACTTTGTACAAGAAAGCTGGGTTCAGATCATACACTTCTAATTGCTT 3’
其中所述引物包括用于Gateway重组的AttB位点。也使用标准方法纯化扩增的PCR片段。随后进行Gateway方法的第一步骤,即BP反应,在此期间所述PCR片段与pDONR201质粒在体内重组以产生根据Gateway术语学的“进入克隆”,pTCP1或pTCP2。质粒pDONR201作为技术的部分从Invitrogen购买。
包含SEQ ID NO:1或SEQ ID NO:2的进入克隆随后在LR反应中与用于稻转化的目的载体一起使用。这种载体含有在T-DNA边界内部的植物选择性标记、可筛选标记表达盒和意图与已经克隆在该进入克隆中的目的核酸序列发生LR体内重组的Gateway盒作为功能性元件。用于组成型表达的稻GOS2启动子或HMGP启动子位于该Gateway盒上游。
在LR重组步骤后,将所得表达载体根据本领域熟知的方法转化至农杆菌菌株LBA4044中。
底物蛋白-样序列的克隆
使用定制的拟南芥幼苗cDNA文库(在pCMV Sport 6.0中;Invitrogen,Paisley,UK)作为模板,通过PCR扩增本在本发明方法中使用的核酸序列。使用Hifi Taq DNA聚合酶,在标准条件下使用50μl PCR混合物中的200ng模板进行PCR。使用的引物是prm09481(SEQ ID NO:46,正义,起始密码子为粗体字):
和prm09482(SEQ ID NO:47;反义,互补):
5’-ggggaccactttgtacaagaaagctgggttcacagacaatttcactgctt-3’,
其中所述引物包括用于Gateway重组的AttB位点。也使用标准方法纯化扩增的PCR片段。随后进行Gateway方法的第一步骤,即BP反应,在此期间所述PCR片段与pDONR201质粒在体内重组以产生根据Gateway术语学的“进入克隆”p底物蛋白-样。质粒pDONR201作为技术的部分从Invitrogen购买。
包含SEQ ID NO:43的进入克隆随后在LR反应中与用于稻转化的目的载体一起使用。这种载体含有在T-DNA边界内部的植物选择性标记、可筛选标记表达盒和意图与已经克隆在该进入克隆中的目的核酸序列发生LR体内重组的Gateway盒作为功能性元件。用于根特异性表达的稻GOS2启动子(SEQ ID NO:45)位于该Gateway盒上游。
在LR重组步骤后,将所得表达载体pGOS2::底物蛋白-样(图4)根据本领域熟知的方法转化至农杆菌菌株LBA4044中。
SEQ ID NO:143所示的核酸序列的克隆
除非另外说明,重组DNA技术根据(Sambrook(2001)MolecularCloning:a laboratory manual,第3版,Cold Spring Harbor LaboratoryPress,CSH,New York)或在Ausubel等(1994),Current Protocols inMolecular Biology,Current Protocols第1卷和第2卷中描述的标准方案进行。用于植物分子研究工作的标准材料和方法在BIOS ScientificPublications Ltd(英国)和Blackwell Scientific Publications(英国)出版的R.D.D.Croy的Plant Molecular Biology Labfax(1993)中描述。
编码IPPT多肽序列的聚球藻属物种PCC 7942核酸序列使用下述基因组DNA作为模板进行PCR扩增,所述基因组DNA提取自聚球藻属物种PCC 7942。包括用于Gateway重组的AttB位点的下列引物用于PCR扩增:
1)Prm 07646(SEQ ID NO:206,正义):
2)Prm 07645(SEQ ID NO:207,反义,互补):
使用Hifi Taq DNA聚合酶在标准条件下进行PCR。使用标准方法扩增和纯化具有预期长度的PCR片段(包含attB位点)。随后进行Gateway方法的第一步骤,即BP反应,在此期间,所述PCR片段与pDONR201质粒在体内重组以产生根据Gateway术语学的“进入克隆”。质粒pDONR201作为技术的部分从Invitrogen购买。
SHR核酸序列的克隆(SEQ ID NO:208)
本发明方法中所用的核酸序列通过PCR,使用定制的拟南芥幼苗cDNA文库(在pCMV Sport 6.0内;Invitrogen,Paisley,UK)作为模板进行扩增。使用Hifi Taq DNA聚合酶,在标准条件下利用在50μl PCR混合物中的200ng模板进行PCR。使用的引物是(SEQ ID NO:243;正义,起始密码子为粗体字):
其中所述引物包括用于Gateway重组的AttB位点。也使用标准方法纯化扩增的PCR片段。随后进行Gateway方法的第一步骤,即BP反应,在此期间所述PCR片段与pDONR201质粒在体内重组以产生根据Gateway术语学的“进入克隆”pSHR。质粒pDONR201作为技术的部分从Invitrogen购买。
包含SEQ ID NO:208的进入克隆随后在LR反应中与用于稻转化的目的载体p00640一起使用。这种载体含有在T-DNA边界内部的植物选择性标记、可筛选标记表达盒和意图与已经克隆在该进入克隆中的目的核酸序列发生LR体内重组的Gateway盒作为功能性元件。用于组成型表达的稻GOS2启动子(SEQ ID NO:242)位于该Gateway盒上游。
在所述LR重组步骤后,所得表达载体pGOS2::SHR(图16)根据本领域熟知的方法转化至农杆菌菌株LBA4044内。
实施例8:使用SEQ ID NO:143所示的核酸序列构建表达载体
包含SEQ ID NO:143的进入克隆随后在LR反应中与用于稻转化法的目的载体一起使用。该载体含有在T-DNA边界内部的植物选择性标记、可筛选标记表达盒,和意图与已经克隆在该进入克隆中的目的核酸序列发生LR体内重组的Gateway盒作为功能性元件。用于种子特异性表达的稻脱水蛋白启动子(SEQ ID NO:204)位于该Gateway盒上游。也产生用于稻转化的第二目的载体,其具有也用于种子特异性表达的稻蛋白酶抑制剂启动子(SEQ ID NO:205)。
在LR重组步骤后,用于种子特异性表达的所得表达载体pDehydrin::IPPT和pProt_inhib::IPPT(图4)根据本领域熟知的方法独立转化至农杆菌菌株LBA4044中。
实施例9:植物转化
稻转化
使用含有表达载体的农杆菌来转化稻植物。将粳稻栽培品种日本晴(Nipponbare)的成熟干燥种子脱壳。通过如下方式实施消毒:在70%乙醇中孵育1分钟,随后在0.2%HgCl2中孵育30分钟,随后用无菌蒸馏水洗涤6次15分钟。无菌的种子随后在含有2,4-D的培养基(愈伤组织诱导培养基)上萌发。在黑暗中孵育4周后,将胚发生的盾片衍生性愈伤组织切下并在相同的培养基上增殖。2周后,将所述愈伤组织通过在同一种培养基上传代培养另外2周进行繁殖或增殖。胚发生的愈伤组织片在新鲜培养基上传代培养3日,随后共培育(以助长细胞分裂活性)。
将含有表达载体的农杆菌菌株LBA4404用于共培育。农杆菌接种在含有适宜抗生素的AB培养基上并在28℃培养3日。随后收集细菌并在液体共培育培养基中悬浮至密度(OD600)约1。该混悬液随后转移至培养皿内并将所述愈伤组织浸入此混悬液中15分钟。该愈伤组织随后在滤纸上蘸干并转移至固化的共培育培养基,并在25℃于黑暗中孵育3日。共培育的愈伤组织在含2,4-D的培养基上在28℃于黑暗中在存在选择剂时培育4周。在此期间,迅速生长的抗性愈伤组织团发育。将这种材料转移至再生培养基并在光照下孵育后,胚发生潜能释放并且苗在随后4至5周内发育。将苗从愈伤组织上切下并且在含有植物生长素的培养基上孵育2至3周,将苗从所述培养基转移至土壤。硬化的苗在温室中于高湿度和短日照下培育。
对于一个构建体,产生大约35个独立的T0稻转化体。将原代转化体从组织培养箱转移至温室。在定量PCR分析验证T-DNA插入物的拷贝数后,仅保留显示所述选择剂抗性的单拷贝转基因植物用于收获T1种子。种子随后在移栽后3至5个月收获。该方法以超过50%的比例产生单基因座转化体(Aldemita和Hodges1996,Chan等1993,Hiei等1994)。
玉米转化
玉米(玉蜀黍,Zea mays)的转化用Ishida等(1996)Nature Biotech 14(6):745-50描述的改良方法进行。在谷物中,转化是基因型依赖的并且仅特定基因型适合于转化和再生。近交系A188(明尼苏达大学)或以A188作为亲本的杂交体是用于转化的供体材料的良好来源,不过也可以成功地使用其他基因型。谷穗从授粉后大约11日(DAP)的谷物植物收获,此时不成熟的胚的长度是大约1至1.2mm。将不成熟的胚与含有所述表达载体的根瘤农杆菌共培育,并且通过器官发生过程回收转基因植物。切下的胚在愈伤组织诱导培养基上、随后在玉米再生培养基上培育,其中所述的培养基含有选择剂(例如咪唑啉酮,不过可以使用不同的选择性标记)。培养板在25℃于光照下孵育2-3周,或直至苗发育。将来自每个胚的绿色苗转移至玉米生根培养基并在25℃孵育2-3周,直至根发育。将生根的苗移植至温室中的土壤内。从针对所述选择剂显示耐受性并且含有单拷贝T-DNA插入物的植物产生T1种子。
小麦转化
小麦的转化用Ishida等(1996)Nature Biotech 14(6):745-50描述的方法进行。栽培品种Bobwhite(可从墨西哥CIMMYT获得)通常用于转化。将不成熟的胚与含有所述表达载体的根瘤农杆菌共培育,并且通过器官发生过程回收转基因植物。与农杆菌孵育后,所述胚在愈伤组织诱导培养基上、随后于再生培养基上体外培育,其中所述的培养基含有选择剂(例如咪唑啉酮,不过可以使用不同的选择性标记)。培养板在25℃于光照下孵育2-3周,或直至苗发育。将来自每个胚的绿色苗转移至生根培养基并在25℃孵育2-3周,直至根发育。将生根的苗移植至温室中的土壤内。从显示选择剂耐受性并且含有单拷贝T-DNA插入物的植物产生T1种子。
大豆转化
根据Texas A&M美国专利5,164,310中描述的改良方法转化大豆。几个商业大豆品种适合于通过这种方法转化。栽培品种Jack(从Illinois种子基金会可获得)通常用于转化。将大豆种子消毒以体外播种。从7日龄年幼籽苗切除下胚轴、胚根和一片子叶。进一步培育上胚轴和剩余的子叶以发育腋生结节。将这些腋生结节切下并与含有表达载体的根瘤农杆菌孵育。在共培育处理之后,将外植体洗涤并转移至选择培养基。将再生的苗切下并置于苗伸长培养基上。将长度不超过1cm的苗置于生根培养基上直至根发育。将生根的苗移植至温室中的土壤内。从显示选择剂耐受性并且含有单拷贝T-DNA插入物的植物产生T1种子。
油菜籽/卡诺拉油菜转化
使用5-6日龄年幼籽苗的子叶柄和下胚轴作为组织培养用外植体并且根据Babic等,(1998,Plant Cell Rep 17:183-188)进行转化。商业品种Westar(Agriculture Canada)是用于转化的标准品种,不过也可以使用其他品种。对卡诺拉油菜种子进行表面消毒以体外播种。从所述体外籽苗切下带有子叶的子叶柄外植体,并且用(含有表达载体的)农杆菌通过该叶柄外植体的切口末端浸入细菌悬液而接种。所述外植体随后在23℃,16小时光照下于含有3mg/l BAP、3%蔗糖、0.7%植物琼脂的MSBAP-3培养基上培养2日。与农杆菌共培育2日后,将叶柄外植体转移至含有3mg/l BAP、头孢噻肟、羧苄青霉素或特美汀(300mg/l)的MSBAP-3培养基上培养7日,并且随后在含有头孢噻肟、羧苄青霉素或特美汀和选择剂的MSBAP-3培养基上培养,直至苗再生。当苗长度是5-10mm时,将这些苗切下并转移至苗伸长培养基(MSBAP-0.5,含有0.5mg/l BAP)。将长度大约2cm的苗转移至用于根诱导的生根培养基(MS0)。将生根的苗移植至温室中的土壤内。从显示选择剂耐受性并且含有单拷贝T-DNA插入物的植物产生T1种子。
苜蓿转化
使用(McKersie等,1999Plant Physiol 119:839-847)的方法转化苜蓿的再生性克隆。苜蓿的再生和转化是基因型依赖性的并且因而需要再生性植物。已经描述了获得再生性植物的方法。例如,这些再生性植物可以选自栽培品种Rangelander(Agriculture Canada)或如Brown DCW和AAtanassov(1985.Plant Cell Tissue Culture 4:111-112)所述的任何其他商业苜蓿品种。备选地,已经选择RA3品种(威斯康星大学)用于组织培养(Walker等,1978Am J Bot 65:654-659)。叶柄外植体与含有表达载体的根瘤农杆菌C58C1pMP90(McKersie等,1999Plant Physiol 119:839-847)或LBA4404的过夜培养物共培育。所述外植体在黑暗中于含有288mg/LPro、53mg/L硫代脯氨酸、4.35g/L K2SO4和100μm乙酰丁香酮的SH诱导培养基上共培育3日。所述外植体在半浓度的Murashige-Skoog培养基(Murashige和Skoog,1962)中洗涤并铺种在不含乙酰丁香酮而含有抑制农杆菌生长的合适选择剂和合适抗生素的相同SH诱导培养基上。几周后,将体细胞胚转移至不含生长调节剂、不含抗生素和含有50g/L蔗糖的BOi2Y发育培养基。随后在半浓度Murashige-Skoog培养基上萌发体细胞胚。将生根的籽苗移植至花钵内并且在温室中培育。从显示选择剂耐受性并且含有单拷贝T-DNA插入物的植物产生T1种子。
棉花转化(对于TCP1/TCP2和底物蛋白-样序列)
使用根瘤农杆菌,根据US 5,159,135中所述的方法转化棉花。棉花种子在3%次氯酸钠溶液中作20分钟表面消毒并在含500μg/ml头孢噻肟的蒸馏水中洗涤。随后将种子转移至含有50μg/m苯菌灵的SH培养基用于萌发。取下4至6日龄籽苗的下胚轴,切成0.5cm小片并置于0.8%琼脂上。农杆菌混悬液(每毫升大约108个细胞,从含有用目的基因和合适选择性标记转化的过夜培养物稀释)用于接种下胚轴外植体。在室温和光照下3日后,将组织转移至固体培养基(1.6g/l脱乙酰吉兰糖胶),所述固体培养基含有具有维生素B5的Murashige和Skoog盐(Gamborg等,Exp.Cell Res.50:151-158(1968))、0.1mg/l 2,4-D、0.1mg/l 6-呋喃甲基氨基嘌呤和750μg/ml MgCL2以及杀死残留细菌的50至100μg/ml头孢噻肟和400-500μg/ml羧苄青霉素。单个细胞系在2至3个月(每隔4至6周传代培养)后分离并且在用于组织增殖的选择培养基上进一步培育(30℃,16小时光周期)。转化的组织随后在非选择培养基上进一步培育持续2至3个月以产生体细胞胚。将至少4mm长度的外观健康胚转移至含有精细蛭石中具SH培养基的管内,所述SH培养基补充有0.1mg/l吲哚乙酸、6-呋喃甲基氨基嘌呤和赤霉酸。在30℃以16小时光周期培育胚,并且将处于2至3叶期的小植物转移至具有蛭石和养分的花钵。植物硬化并随后移至温室以进一步培育。
棉花转化(对于IPPT)
棉花(陆地棉)的转化使用根瘤农杆菌,在下胚轴外植体上进行。市售品种,例如Coker 130或Coker 312(SeedCo,Lubbock,TX)是用于转化的标准变种,但是也可以使用其他变种。表面消毒种子并在黑暗中萌发。从长约1-1.5厘米的萌发幼苗上切下下胚轴外植体。下胚轴外植体浸没在包含表达载体的根瘤农杆菌接种物中5分钟,然后在黑暗中,于24℃下在MS+1.8mg/l KNO3+2%葡萄糖上共培育约48小时。外植体转移到含有合适细菌和植物选择性标记的相同培养基中(更新几次),直到可以看见胚性胼胝体(embryogenic calli)。分离胼胝体并再培养直至出现体细胞胚。源自体细胞胚的小植株在生根培养基上成熟至根发育。生根的幼苗转移到温室中的花钵土壤中。从表现选择剂耐受性并含有单拷贝T-DNA插入物的植物中产生T1种子。
实施例10:表型评价方法
9.1评价建立
产生大约35个独立的T0稻转化体。将原代转化体从组织培养室转移至温室以培育并收获T1种子。留下6个事件,其中T1子代以3∶1比例对所述转基因的存在/不存在分离。对于这些事件中的每个事件,通过监测目视标记表达选出大约10株含有该转基因的T1籽苗(杂合子和纯合子)和大约10株缺少该转基因的T1籽苗(失效合子)。以随机位置并排生长转基因植物和相应的失效合子。温室条件是短日照(12小时光照),在光照下28℃和在黑暗中22℃,和70%相对湿度。
如果要核实一遍,4个T1事件在T2世代中按照如对T1世代相同的评价方法进一步评估,但是每个事件采用更多个体。植物从播种期至成熟期数次通过数字成像室。在每个时间点上,从至少6个不同角度拍摄每株植物的数字图像(2048×1536像素,1600万颜色)。
4个T1事件在T2世代中按照如对T1世代相同的评价方法进一步评估,但是每个事件采用更多个体。植物从播种期至成熟期数次通过数字成像室。在每个时间点上,从至少6个不同角度拍摄每株植物的数字图像(2048×1536像素,1600万颜色)。
干旱筛选(对于TCP1/TCP2和SHR)
对于TCP1/TCP2,在盆栽土壤中在正常条件下培育来自T2种子的植物直至它们接近齐穗期。
对于SHR,在盆栽土壤中在正常条件下培育来自T2种子的植物直至它们接近齐穗期。
随后将它们转移至灌溉减少的“干燥”区。将湿度探测器插入随机选择的花钵内,以监测土壤水含量(SWC)。当SWC下降低于某个阈值时,自动地对所述植物连续再灌溉直至再次达到正常水平。随后将植物再次转移至正常条件。栽培的剩余部分(植物成熟、种子收获)与不在非生物胁迫条件下培育的植物相同。如对正常条件下详述那样记录生长和产量参数。
干旱筛选(底物蛋白-样序列)
在盆栽土壤中在正常条件下培育来自T2种子的植物直至它们接近齐穗期。随后将它们转移至灌溉减少的“干燥”区。将湿度探测器插入随机选择的花钵内,以监测土壤水含量(SWC)。当SWC下降低于某个阈值时,自动地对所述植物连续再灌溉直至再次达到正常水平。随后将植物再次转移至正常条件。栽培的剩余部分(植物成熟、种子收获)与不在非生物胁迫条件下培育的植物相同。如对正常条件下详述那样记录生长和产量参数。
干旱筛选(IPPT)
在盆栽土壤中在正常条件下培育来自被选择数量事件的植物直至它们接近齐穗期。随后将它们转移至灌溉减少的“干燥”区。将湿度探测器插入随机选择的花钵内,以监测土壤水含量(SWC)。当SWC下降低于某个阈值时,自动地对所述植物连续再灌溉直至再次达到正常水平。随后将植物再次转移至正常条件。栽培的剩余部分(植物成熟、种子收获)与不在非生物胁迫条件下培育的植物相同。如对正常条件下详述那样记录生长和产量参数。
氮利用效率筛选(对于TCP1/TCP2)
在盆栽土壤中在除营养液之外的正常条件下培育来自T2种子的稻植物。从移植至成熟期间用含有降低的、通常7至8倍之间更少的氮(N)含量的特定营养液浇灌所述花钵。栽培的剩余部分(植物成熟、种子收获)与不在非生物胁迫下培育的植物相同。如对正常条件下详述那样记录生长和产量参数。
氮利用效率筛选(对于底物蛋白-样序列)
在盆栽土壤中在除营养液之外的正常条件下培育来自T2种子的稻植物。从移植至成熟期间用含有降低的、通常7至8倍之间更少的氮(N)含量的特定营养液浇灌所述花钵。栽培的剩余部分(植物成熟、种子收获)与不在非生物胁迫下培育的植物相同。如对正常条件下详述那样记录生长和产量参数。
氮利用效率筛选(对于SHR)
在盆栽土壤中在除营养液之外的正常条件下培育来自T2种子的稻植物。从移植至成熟期间用含有降低的、通常7至8倍之间更少的氮(N)含量的特定营养液浇灌所述花钵。栽培的剩余部分(植物成熟、种子收获)与不在非生物胁迫下培育的植物相同。如对正常条件下详述那样记录生长和产量参数。
盐胁迫筛选(对于底物蛋白-样序列)
植物在由椰子纤维和argex(3∶1比率)组成的基质上培育。在温室中移植小植物后,在头两周期间使用正常营养液。在这两周后,添加25mM盐(NaCl)至所述营养液,直至收获植物。随后测量种子相关参数。
盐胁迫筛选(对于IPPT)
植物在由椰子纤维和argex(3∶1比率)组成的基质上培育。在温室中移植小植物后,在头两周期间使用正常营养液。在这两周后,添加25mM盐(NaCl)至所述营养液,直至收获植物。如对正常条件下详述那样记录生长和产量参数。
降低的营养(氮)可利用性筛选(对于IPPT)
在除营养液之外的正常条件下在盆栽土壤中培育来自6个事件(T2种子)的植物。从移植至成熟期间用含有降低的、通常7至8倍之间更少的氮(N)含量的特定营养液浇灌所述花钵。培育的其余部分(植物成熟、种子收获)与不在非生物胁迫下培育的植物相同。如对正常条件下详述那样记录生长和产量参数。
9.2统计分析:F-检验
使用两因素ANOVA(变量分析)作为总体评价植物表型特征的统计模型。对用本发明基因转化的全部事件的全部植物的全部所测量参数实施F检验。实施F检验以检查该基因对全部转化事件的影响并验证该基因的整体作用(又称作基因总体作用)。对于该F检验而言,真实基因总体作用显著性的阈值对于所述F检验设置在5%概率水平上。显著性F检验值指出了基因作用,这意味不仅仅是基因的存在或位置才造成表型上的差异。
因为实施了具有重叠事件的两个实验,故进行联合分析。这用于检验对这两个实验影响的一致性,并且如果一致,则用于积累来自两个实验的证据以提高结论的可信度。所用的方法是考虑数据的多重水平结构的混合模型法(即实验-事件-分离子)。通过比较似然比检验与卡方分布(chi squaredistribution)获得P-值。
9.3测量的参数
生物量相关的参数测量
植物从播种期至成熟期数次通过数字成像室。在每个时间点上,从至少6个不同角度拍摄每株植物的数字图像(2048×1536像素,1600万颜色)。
植物地上部分面积(或叶生物量)通过计数来自植物地上部分的数字图像上与背景区别的像素总数而确定。这个值对相同时间点上从不同角度拍摄的画面进行平均化并且通过校正转化成以平方mm表述的物理表面值(physical surface value)。实验显示以这种方式测量的地上部分植物面积与地上植物部分的生物量相关。地上部分面积是在植物已经达到其最大叶生物量的时间点处所测量的面积。早期生长势是萌发后3周的植物(籽苗)地上部分面积。根生物量的增加表述为总根生物量的增加(测量为植物寿命期间所观察到的根最大生物量);或表述为根/冠比增加(测量为根和苗的活跃生长期间根质量与苗质量之间的比例)。
早期生长势通过计数来自植物地上部分的与背景区别的像素总数确定。这个值对相同时间点上从不同角度拍摄的画面进行平均化并且通过校正转化成以平方mm表述的物理表面值。下述结果是针对萌发后3周的植物。
种子相关的参数测量值
将成熟的原发花序收获、计数、装袋、加条形码标记并且随后在干燥箱内于37℃干燥3日。随后将所述花序脱粒,并且收集和计数全部种子。使用吹气装置将饱满粒与空粒分开。弃去空粒并且再次计数剩余部分。饱满粒在分析天平上称重。通过计数分离步骤后仍留下的饱满粒的数目确定饱满种子数。种子总产量通过称量从一株植物收获的全部饱满粒测量。每株植物的种子总数通过计数从一株植物收获的壳数测量。千粒重(TKW)从计数的饱满种子数及它们的总重量外推出来。本发明中的收获指数(HI)定义为种子总产量与地上部分面积(mm2)之间的比率乘以系数106。如本发明中定义的每花序总花数是种子总数与成熟原发花序数之间的比率。如本发明中定义的种子饱满率是饱满种子数对种子(或小花)总数的比例(表述为%)。
实施例11:转基因植物的表型评测结果
表达TCP1或TCP2核酸的转基因稻植物的评测结果如下所示。百分比差异是转基因植物与对应的失效合子的比较。
表达构建体pHMGP::TCP1(苜蓿)或pGOS2::TCP1(苜蓿)的稻植物在非胁迫和干旱条件下的评测结果。
干旱 | 非胁迫 | |
pHMGP::TCP1 | pHMGP::TCP1 | |
种子总重量 | 42% | 11% |
饱满种子数 | 43% | 9% |
饱满率 | 22% | |
每穗花数 | 7% | 4% |
第一花穗数 | 7% | |
收获指数 | 36% | 7% |
地上部分面积 | <4% | |
萌发势 | 8% | |
TKW | <5% |
对于在非胁迫条件下的构建体pGOS2::TCP1(苜蓿),发现在以下参数上有正趋势:萌发势、种子总重量和TKW。
表达构建体或pGOS2::TCP2(苜蓿)的稻植物在非胁迫和干旱条件下的评测结果。
下面提供了表达底物蛋白-样核酸的转基因稻植物的评测结果。至少5%的提高在种子总数、种子总产量、饱满种子数和饱满率中观察到。此外,T1和T2世代中都至少有一个事件的地上部分生物量和早期生长势有超过5%的提高。
表E:表达SEQ ID NO:44的底物蛋白-样核酸的植物中发现的产量提高:
表达在种子特异性脱水蛋白启动子调控下,编码SEQ ID NO:144所示的IPPT多肽的核酸序列的转基因稻植物的表型评测结果。
下面提供表达在种子特异性脱水蛋白启动子调控下,编码SEQ ID NO:144所示的IPPT多肽的核酸序列且在正常生长条件下培育的T1和T2世代转基因稻植物的评测结果。
与对应的失效合子(对照植物)相比,所述转基因植物的早期生长势、地上部分生物量、每株植物种子总产量、种子总数量、饱满种子数、和每穗花数和收获指数有显著提高,如表F所示。
表F:表达在种子特异性脱水蛋白启动子调控下,编码SEQ ID NO:144所示的IPPT多肽的核酸序列的T1和T2世代转基因稻植物的评测结果。
性状 | T1世代中6个事件的总体平均提高% | T2世代中4个事件的总体平均提高% |
早期生长势 | 25 | 25 |
地上部分生物量 | 2 | 8 |
每株植物种子总产量 | 14 | 13 |
种子总数量 | 8 | 15 |
总饱满种子数 | 15 | 13 |
收获指数 | 14 | 5 |
第一花穗数 | 13 | 3 |
表达在种子特异性蛋白酶抑制剂启动子调控下,编码SEQ ID NO:144所示的IPPT多肽的核酸序列的转基因稻植物的表型评测结果。
下面提供表达在种子特异性蛋白酶抑制剂启动子调控下,编码SEQ IDNO:144所示的IPPT多肽的核酸序列且在正常生长条件下培育的T1世代转基因稻植物的评测结果。
与对应的失效合子(对照植物)相比,所述转基因植物的早期生长势、地上部分生物量、每株植物种子总产量、种子总数量、饱满种子数和每穗花数有显著提高,如表G所示。
表G:表达在种子特异性蛋白酶抑制剂启动子调控下,编码SEQ IDNO:144所示的IPPT多肽的核酸序列的T1世代转基因稻植物的评测结果。
性状 | T1世代中2个最佳事件的总体平均提高% |
早期生长势 | 34 |
地上部分生物量 | 15 |
每株植物种子总产量 | 20 |
种子总数量 | 23 |
饱满种子数 | 18 |
第一花穗数 | 22 |
下面提供非胁迫条件下表达SHR核酸的转基因稻植物的评测结果。
参数 | 与对照植物的%差异 |
TKW | 7.3% |
下面提供降低的养分可利用性条件下表达SHR核酸的转基因稻植物的评测结果。
参数 | 与对照植物的%差异 |
地上部分面积 | 10.2% |
萌发势 | 23.2% |
根生物量 | 23.6% |
饱满率 | 25.3% |
TKW | 7% |
序列表
<110>巴斯夫植物科学有限公司(BASF Plant Science GmbH)
<120>具有增强的产量相关性状的植物及其制备方法
<130>PF60399
<160>244
<170>PatentIn版本3.5
<210>1
<211>708
<212>DNA
<213>苜蓿(Medicago sativa)
<400>1
atgtctaacc acaaggaaac aacaactatc accacaacaa ccgatctagt agaacccact 60
tctcaaaacg ctataacagt aaaaaaaccg ccgtcaaaag accgtcacag taaagtagac 120
ggacgaggaa gacgcataag aatgcctata atttgcgccg cacgtgtttt ccagttaaca 180
cgtgagcttg gtcataaatc cgacggtcaa actattgaat ggcttctccg tcaagctgaa 240
ccgtcgatca tcgccgccac cggtaccgga actactcctg cttctttctc ttcgctttct 300
gagcataaac cttttcttca accttctcct tttattcttg gtaaacgttt acgtgatgat 360
gatggtgatg ctgtttcggt tgccgcacct actgctccgc cggggttgtg gcggccggat 420
tttgctcagc tttggagctt tgctgcggct gctcaaactc cgccggaagg tttttctgtt 480
tctcagcata attcattgtt tcatcatcaa cagcaacaac aacaacaaca gcaccagcaa 540
cagcaacaac agcaccacca ccagcagcat caccagcagc aacaaggctc tatgggtgaa 600
gcttccgcgg cgaggttagg gaattatctt cctggtcatc tgaatttgtt ggcttcattg 660
tctggtggga atggaaattc tggtcgtgga gatgatgaag atcgttga 708
<210>2
<211>235
<212>PRT
<213>苜蓿
<400>2
Met Ser Asn His Lys Glu Thr Thr Thr Ile Thr Thr Thr Thr Asp Leu
1 5 10 15
Val Glu Pro Thr Ser Gln Asn Ala Ile Thr Val Lys Lys Pro Pro Ser
20 25 30
Lys Asp Arg His Ser Lys Val Asp Gly Arg Gly Arg Arg Ile Arg Met
35 40 45
Pro Ile Ile Cys Ala Ala Arg Val Phe Gln Leu Thr Arg Glu Leu Gly
50 55 60
His Lys Ser Asp Gly Gln Thr Ile Glu Trp Leu Leu Arg Gln Ala Glu
65 70 75 80
Pro Ser Ile Ile Ala Ala Thr Gly Thr Gly Thr Thr Pro Ala Ser Phe
85 90 95
Ser Ser Leu Ser Glu His Lys Pro Phe Leu Gln Pro Ser Pro Phe Ile
100 105 110
Leu Gly Lys Arg Leu Arg Asp Asp Asp Gly Asp Ala Val Ser Val Ala
115 120 125
Ala Pro Thr Ala Pro Pro Gly Leu Trp Arg Pro Asp Phe Ala Gln Leu
130 135 140
Trp Ser Phe Ala Ala Ala Ala Gln Thr Pro Pro Glu Gly Phe Ser Val
145 150 155 160
Ser Gln His Asn Ser Leu Phe His His Gln Gln Gln Gln Gln Gln Gln
165 170 175
Gln His Gln Gln Gln Gln Gln Gln His His His Gln Gln His His Gln
180 185 190
Gln Gln Gln Gly Ser Met Gly Glu Ala Ser Ala Ala Arg Leu Gly Asn
195 200 205
Tyr Leu Pro Gly His Leu Asn Leu Leu Ala Ser Leu Ser Gly Gly Asn
210 215 220
Gly Asn Ser Gly Arg Gly Asp Asp Glu Asp Arg
225 230 235
<210>3
<211>1251
<212>DNA
<213>蒺藜苜蓿(Medicago truncatula)
<400>3
atggaattgg aaggtgatca tcatcaaagt aatggaatcc gaagcagacc caatttccct 60
cttcagcttt tagagaaaaa agacaacaac atcctagatg tctccgaaga acaaacttgt 120
acaaccaccg gaaacgatgg ctgtactgcc ataatctcat cagaccagca gaaaaagcct 180
ccgccgaagc gagcttcgac gaaagaccgg cacacgaagg tagacggacg aggccgccgg 240
atccgtatgc cggcggcctg tgctgctaga gtgttccagc tcacgcgtga gctgggacat 300
aaatcagacg gcgagacgat tgagtggctg cttcaacagg cagagccagc cgtcatcgcc 360
gccaccggaa caggaacaat tccggccaac tttacttcac ttaatatatc tcttagaagc 420
tcaggctcaa ccatgtctgc gccgtctcac tattttcgag gtaactactt taaccctagc 480
tcgttctcct ccacggcggc tcagcttaga aaccgggctg aatgggatag aactatgaac 540
atgaacatga acatgaacat gaacgttgta gctgatgatt caagaaggag tagtaactct 600
accatgttgg aaaatccttc ttctttatca gcaatgttga atttcaacaa cgtgcaaaat 660
gctattctac aagcaaaaca agagcttcgg gaagaaaacg ccgctgccac cgcctccggt 720
ggtggcggag gatttgaatt gttaggttca gattcagatg gaagcatggg aagaaaaaga 780
aggtcagagc aagaattatt atcacaaaat atgggaagtt atttgttaca atcaaatgta 840
gggtcacttc cttcaagtca tgcttcaaat acggcagcgt tttggatggt aactggtaat 900
gggaaccaag gtgttaatgt taatggtggt aatggaaatg atccaatttg ggcaattcct 960
tctgttggga ataatggtat gtatagagga gctatgacat ctcctaatgg atttcatttc 1020
atgaattttg ctgcatctcc aatgaatctt atgccaggaa gtcaattagg ttcaggaatc 1080
gtcggcggtg gtggtggagg tattttagcc gaaagcaatc tgagtatgct ttcggctatg 1140
aatgcctata ggcagatttc gacgaatggt atgtcaaatt cgtcggggca acatcacggc 1200
ggaggtggag gaggagatga tgaacatgac tcaagcagtc aacattccta a 1251
<210>4
<211>416
<212>PRT
<213>蒺藜苜蓿
<400>4
Met Glu Leu Glu Gly Asp His His Gln Ser Asn Gly Ile Arg Ser Arg
1 5 10 15
Pro Asn Phe Pro Leu Gln Leu Leu Glu Lys Lys Asp Ash Asn Ile Leu
20 25 30
Asp Val Ser Glu Glu Gln Thr Cys Thr Thr Thr Gly Asn Asp Gly Cys
35 40 45
Thr Ala Ile Ile Ser Ser Asp Gln Gln Lys Lys Pro Pro Pro Lys Arg
50 55 60
Ala Ser Thr Lys Asp Arg His Thr Lys Val Asp Gly Arg Gly Arg Arg
65 70 75 80
Ile Arg Met Pro Ala Ala Cys Ala Ala Arg Val Phe Gln Leu Thr Arg
85 90 95
Glu Leu Gly His Lys Ser Asp Gly Glu Thr Ile Glu Trp Leu Leu Gln
100 105 110
Gln Ala Glu Pro Ala Val Ile Ala Ala Thr Gly Thr Gly Thr Ile Pro
115 120 125
Ala Asn Phe Thr Ser Leu Asn Ile Ser Leu Arg Ser Ser Gly Ser Thr
130 135 140
Met Ser Ala Pro Ser His Tyr Phe Arg Gly Asn Tyr Phe Asn Pro Ser
145 150 155 160
Ser Phe Ser Ser Thr Ala Ala Gln Leu Arg Asn Arg Ala Glu Trp Asp
165 170 175
Arg Thr Met Asn Met Asn Met Asn Met Asn Met Asn Val Val Ala Asp
180 185 190
Asp Ser Arg Arg Ser Ser Asn Ser Thr Met Leu Glu Asn Pro Ser Ser
195 200 205
Leu Ser Ala Met Leu Asn Phe Asn Asn Val Gln Asn Ala Ile Leu Gln
210 215 220
Ala Lys Gln Glu Leu Arg Glu Glu Asn Ala Ala Ala Thr Ala Ser Gly
225 230 235 240
Gly Gly Gly Gly Phe Glu Leu Leu Gly Ser Asp Ser Asp Gly Ser Met
245 250 255
Gly Arg Lys Arg Arg Ser Glu Gln Glu Leu Leu Ser Gln Asn Met Gly
260 265 270
Ser Tyr Leu Leu Gln Ser Asn Val Gly Ser Leu Pro Ser Ser His Ala
275 280 285
Ser Asn Thr Ala Ala Phe Trp Met Val Thr Gly Asn Gly Asn Gln Gly
290 295 300
Val Asn Val Asn Gly Gly Asn Gly Asn Asp Pro Ile Trp Ala Ile Pro
305 310 315 320
Ser Val Gly Asn Asn Gly Met Tyr Arg Gly Ala Met Thr Ser Pro Asn
325 330 335
Gly Phe His Phe Met Asn Phe Ala Ala Ser Pro Met Asn Leu Met Pro
340 345 350
Gly Ser Gln Leu Gly Ser Gly Ile Val Gly Gly Gly Gly Gly Gly Ile
355 360 365
Leu Ala Glu Ser Asn Leu Ser Met Leu Ser Ala Met Asn Ala Tyr Arg
370 375 380
Gln Ile Ser Thr Asn Gly Met Ser Asn Ser Ser Gly Gln His His Gly
385 390 395 400
Gly Gly Gly Gly Gly Asp Asp Glu His Asp Ser Ser Ser Gln His Ser
405 410 415
<210>5
<211>2193
<212>DNA
<213>人工序列
<220>
<223>启动子
<400>5
aatccgaaaa gtttctgcac cgttttcacc ccctaactaa caatataggg aacgtgtgct 60
aaatataaaa tgagacctta tatatgtagc gctgataact agaactatgc aagaaaaact 120
catccaccta ctttagtggc aatcgggcta aataaaaaag agtcgctaca ctagtttcgt 180
tttccttagt aattaagtgg gaaaatgaaa tcattattgc ttagaatata cgttcacatc 240
tctgtcatga agttaaatta ttcgaggtag ccataattgt catcaaactc ttcttgaata 300
aaaaaatctt tctagctgaa ctcaatgggt aaagagagag atttttttta aaaaaataga 360
atgaagatat tctgaacgta ttggcaaaga tttaaacata taattatata attttatagt 420
ttgtgcattc gtcatatcgc acatcattaa ggacatgtct tactccatcc caatttttat 480
ttagtaatta aagacaattg acttattttt attatttatc ttttttcgat tagatgcaag 540
gtacttacgc acacactttg tgctcatgtg catgtgtgag tgcacctcct caatacacgt 600
tcaactagca acacatctct aatatcactc gcctatttaa tacatttagg tagcaatatc 660
tgaattcaag cactccacca tcaccagacc acttttaata atatctaaaa tacaaaaaat 720
aattttacag aatagcatga aaagtatgaa acgaactatt taggtttttc acatacaaaa 780
aaaaaaagaa ttttgctcgt gcgcgagcgc caatctccca tattgggcac acaggcaaca 840
acagagtggc tgcccacaga acaacccaca aaaaacgatg atctaacgga ggacagcaag 900
tccgcaacaa ccttttaaca gcaggctttg cggccaggag agaggaggag aggcaaagaa 960
aaccaagcat cctcctcctc ccatctataa attcctcccc ccttttcccc tctctatata 1020
ggaggcatcc aagccaagaa gagggagagc accaaggaca cgcgactagc agaagccgag 1080
cgaccgcctt cttcgatcca tatcttccgg tcgagttctt ggtcgatctc ttccctcctc 1140
cacctcctcc tcacagggta tgtgcccttc ggttgttctt ggatttattg ttctaggttg 1200
tgtagtacgg gcgttgatgt taggaaaggg gatctgtatc tgtgatgatt cctgttcttg 1260
gatttgggat agaggggttc ttgatgttgc atgttatcgg ttcggtttga ttagtagtat 1320
ggttttcaat cgtctggaga gctctatgga aatgaaatgg tttagggtac ggaatcttgc 1380
gattttgtga gtaccttttg tttgaggtaa aatcagagca ccggtgattt tgcttggtgt 1440
aataaaagta cggttgtttg gtcctcgatt ctggtagtga tgcttctcga tttgacgaag 1500
ctatcctttg tttattccct attgaacaaa aataatccaa ctttgaagac ggtcccgttg 1560
atgagattga atgattgatt cttaagcctg tccaaaattt cgcagctggc ttgtttagat 1620
acagtagtcc ccatcacgaa attcatggaa acagttataa tcctcaggaa caggggattc 1680
cctgttcttc cgatttgctt tagtcccaga attttttttc ccaaatatct taaaaagtca 1740
ctttctggtt cagttcaatg aattgattgc tacaaataat gcttttatag cgttatccta 1800
gctgtagttc agttaatagg taatacccct atagtttagt caggagaaga acttatccga 1860
tttctgatct ccatttttaa ttatatgaaa tgaactgtag cataagcagt attcatttgg 1920
attatttttt ttattagctc tcaccccttc attattctga gctgaaagtc tggcatgaac 1980
tgtcctcaat tttgttttca aattcacatc gattatctat gcattatcct cttgtatcta 2040
cctgtagaag tttctttttg gttattcctt gactgcttga ttacagaaag aaatttatga 2100
agctgtaatc gggatagtta tactgcttgt tcttatgatt catttccttt gtgcagttct 2160
tggtgtagct tgccactttc accagcaaag ttc 2193
<210>6
<211>1130
<212>DNA
<213>人工序列
<220>
<223>启动子
<400>6
catgcggcta atgtagatgc tcactgcgct agtagtaagg tactccagta cattatggaa 60
tatacaaagc tgtaatactc gtatcagcaa gagagaggca cacaagttgt agcagtagca 120
caggattaga aaaacgggac gacaaatagt aatggaaaaa caaaaaaaaa caaggaaaca 180
catggcaata taaatggaga aatcacaaga ggaacagaat ccgggcaata cgctgcgaaa 240
gtactcgtac gtaaaaaaaa gaggcgcatt catgtgtgga cagcgtgcag cagaagcagg 300
gatttgaaac cactcaaatc caccactgca aaccttcaaa cgaggccatg gtttgaagca 360
tagaaagcac aggtaagaag cacaacgccc tcgctctcca ccctcccacc caatcgcgac 420
gcacctcgcg gatcggtgac gtggcctcgc cccccaaaaa tatcccgcgg cgtgaagctg 480
acaccccggg cccacccacc tgtcacgttg gcacatgttg gttatggttc ccggccgcac 540
caaaatatca acgcggcgcg gcccaaaatt tccaaaatcc cgcccaagcc cctggcgcgt 600
gccgctcttc cacccaggtc cctctcgtaa tccataatgg cgtgtgtacc ctcggctggt 660
tgtacgtggg cgggttaccc tgggggtgtg ggtggatgac gggtgggccc ggaggaggtc 720
cggccccgcg cgtcatcgcg gggcggggtg tagcgggtgc gaaaaggagg cgatcggtac 780
gaaaattcaa attaggaggt ggggggcggg gcccttggag aataagcgga atcgcagata 840
tgcccctgac ttggcttggc tcctcttctt cttatccctt gtcctcgcaa ccccgcttcc 900
ttctctcctc tcctcttctc ttctcttctc tggtggtgtg ggtgtgtccc tgtctcccct 960
ctccttcctc ctctcctttc ccctcctctc ttcccccctc tcacaagaga gagagcgcca 1020
gactctcccc aggtgaggtg agaccagtct ttttgctcga ttcgacgcgc ctttcacgcc 1080
gcctcgcgcg gatctgaccg cttccctcgc ccttctcgca ggattcagcc 1130
<210>7
<211>753
<212>DNA
<213>拟南芥(Arabidopsis thaliana)
<400>7
atgtctatta acaacaacaa caacaacaac aacaataaca acgatggtct tatgatctca 60
tcaaacggag ctttaatcga acaacaacca tcagtcgttg tgaagaaacc accggcgaaa 120
gatcgacata gcaaagtcga tggaagaggg agaagaatcc gtatgccgat tatatgtgct 180
gctcgtgttt ttcagctaac gagagagctt ggtcataagt cagatggcca aacaattgaa 240
tggttacttc gtcaagcaga gccttctatt atagctgcaa caggaactgg tacaactcca 300
gcgagtttct caactgcttc tgtctctatc cgtggagcca ccaattctac ttctttagat 360
cataaaccca cttctttact tggtggtacg tcaccgttta tacttgggaa acgtgttaga 420
gctgatgagg atagtaataa tagtcataat catagttctg ttggtaaaga tgagaccttt 480
acgacaacac cagctgggtt ttgggctgtt ccggcgaggc cggattttgg acaagtttgg 540
agttttgctg gagctccaca agagatgttt ttacaacaac aacatcatca tcagcaacca 600
ttgtttgttc atcagcaaca gcaacaacaa gctgcaatgg gtgaagcttc tgctgctaga 660
gttgggaatt atcttccggg tcatcttaat ttgcttgctt ctttatccgg tggatctccc 720
gggtcggatc gaagagagga agatccacgt taa 753
<210>8
<211>250
<212>PRT
<213>拟南芥
<400>8
Met Ser Ile Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asp Gly
1 5 10 15
Leu Met Ile Ser Ser Asn Gly Ala Leu Ile Glu Gln Gln Pro Ser Val
20 25 30
Val Val Lys Lys Pro Pro Ala Lys Asp Arg His Ser Lys Val Asp Gly
35 40 45
Arg Gly Arg Arg Ile Arg Met Pro Ile Ile Cys Ala Ala Arg Val Phe
50 55 60
Gln Leu Thr Arg Glu Leu Gly His Lys Ser Asp Gly Gln Thr Ile Glu
65 70 75 80
Trp Leu Leu Arg Gln Ala Glu Pro Ser Ile Ile Ala Ala Thr Gly Thr
85 90 95
Gly Thr Thr Pro Ala Ser Phe Ser Thr Ala Ser Val Ser Ile Arg Gly
100 105 110
Ala Thr Asn Ser Thr Ser Leu Asp His Lys Pro Thr Ser Leu Leu Gly
115 120 125
Gly Thr Ser Pro Phe Ile Leu Gly Lys Arg Val Arg Ala Asp Glu Asp
130 135 140
Ser Asn Asn Ser His Asn His Ser Ser Val Gly Lys Asp Glu Thr Phe
145 150 155 160
Thr Thr Thr Pro Ala Gly Phe Trp Ala Val Pro Ala Arg Pro Asp Phe
165 170 175
Gly Gln Val Trp Ser Phe Ala Gly Ala Pro Gln Glu Met Phe Leu Gln
180 185 190
Gln Gln His His His Gln Gln Pro Leu Phe Val His Gln Gln Gln Gln
195 200 205
Gln Gln Ala Ala Met Gly Glu Ala Ser Ala Ala Arg Val Gly Asn Tyr
210 215 220
Leu Pro Gly His Leu Asn Leu Leu Ala Ser Leu Ser Gly Gly Ser Pro
225 230 235 240
Gly Ser Asp Arg Arg Glu Glu Asp Pro Arg
245 250
<210>9
<211>636
<212>DNA
<213>稻(Oryza sativa)
<400>9
atgagctccc gcgacgccgc cgccacgttc catgtctacc agcccgtgca gatcccgacg 60
gccacggtgg cgcccgctgc ggcagtgtcg gcggcgccgg cagaggccgt cgcccagctg 120
gtcccggcgc cgagcaagaa ggcggccggc gcggcgggtg ggaaggaccg gcacagcaag 180
gtgaacgggc gcgggcggcg cgtgcggatg ccgatcgtgt gcgcggcgcg ggtgttccag 240
ctcacgcggg agctggggct caagtccgac ggccagacca tcgagtggct gctccgccag 300
gccgagccgt ccatcctcgc cgccacgggg accggcacca ccccggccgc cttcgtctcc 360
tcctccgcgc cgtccacctc ctcctcgcac cagcacaccc tcctcggcaa gcgccagcgc 420
caggagtccg ccgccgccga cgccgtctcc gtcgccggcg ccgcgtccgc gttctgggcg 480
gcgctgcccg caccaggccg gcccgacgcc tggggcttct ccccgctgga cgcgcagccg 540
acgtacgtgc cgatggcgca ggcgcaccac caccacctca acctcctcgc cgcgctctcc 600
ggcgccgcca ggcgcgccga ggaggagtcc cggtga 636
<210>10
<211>211
<212>PRT
<213>稻
<400>10
Met Ser Ser Arg Asp Ala Ala Ala Thr Phe His Val Tyr Gln Pro Val
1 5 10 15
Gln Ile Pro Thr Ala Thr Val Ala Pro Ala Ala Ala Val Ser Ala Ala
20 25 30
Pro Ala Glu Ala Val Ala Gln Leu Val Pro Ala Pro Ser Lys Lys Ala
35 40 45
Ala Gly Ala Ala Gly Gly Lys Asp Arg His Ser Lys Val Asn Gly Arg
50 55 60
Gly Arg Arg Val Arg Met Pro Ile Val Cys Ala Ala Arg Val Phe Gln
65 70 75 80
Leu Thr Arg Glu Leu Gly Leu Lys Ser Asp Gly Gln Thr Ile Glu Trp
85 90 95
Leu Leu Arg Gln Ala Glu Pro Ser Ile Leu Ala Ala Thr Gly Thr Gly
100 105 110
Thr Thr Pro Ala Ala Phe Val Ser Ser Ser Ala Pro Ser Thr Ser Ser
115 120 125
Ser His Gln His Thr Leu Leu Gly Lys Arg Gln Arg Gln Glu Ser Ala
130 135 140
Ala Ala Asp Ala Val Ser Val Ala Gly Ala Ala Ser Ala Phe Trp Ala
145 150 155 160
Ala Leu Pro Ala Pro Gly Arg Pro Asp Ala Trp Gly Phe Ser Pro Leu
165 170 175
Asp Ala Gln Pro Thr Tyr Val Pro Met Ala Gln Ala His His His His
180 185 190
Leu Asn Leu Leu Ala Ala Leu Ser Gly Ala Ala Arg Arg Ala Glu Glu
195 200 205
Glu Ser Arg
210
<210>11
<211>621
<212>DNA
<213>稻
<400>11
atggcctccc gcgacgccgc caccttccag gtctaccggc ccatggcaat gccaacgccg 60
gcggcgctgc caccgtcgtc ccagcagatc acaatgccgt tcacggcggc gctcgtggac 120
gccgtgctcc cagctccccg aaaggcggcg gcgacgcagg gcggcaagga ccggcacagc 180
aaggtgaacg ggcgcggccg gcgcgtgcgc atgccgatcg tctgcgcggc gcgcgtgttc 240
cagctcaccc gcgagctggg gctcaagtcc gacggccaga ccatcgagtg gctgctccgg 300
caggccgagc cgtccatcct cgccgccacc ggctccggca ccaccccggc cgtcttctcc 360
tgctcgtccg ccccctccac cgcctcctcc tccttcctcc tcggcaagcg cccgcgccag 420
gaggaccacg aggcgccgac gttctgggag gcgctgcagc aacagccgcg gcccgccgtg 480
tcgtcgtggg gtgccctcgt ctcgccgtcg caggaggcgc aggcgtacgc gtcgtcggtg 540
gcgcaggtcc accacctcaa cctactctcc gcgctctccg gcgccgccac gagacggcca 600
gcacaggagg agtcccggtg a 621
<210>12
<211>206
<212>PRT
<213>稻
<400>12
Met Ala Ser Arg Asp Ala Ala Thr Phe Gln Val Tyr Arg Pro Met Ala
1 5 10 15
Met Pro Thr Pro Ala Ala Leu Pro Pro Ser Ser Gln Gln Ile Thr Met
20 25 30
Pro Phe Thr Ala Ala Leu Val Asp Ala Val Leu Pro Ala Pro Arg Lys
35 40 45
Ala Ala Ala Thr Gln Gly Gly Lys Asp Arg His Ser Lys Val Asn Gly
50 55 60
Arg Gly Arg Arg Val Arg Met Pro Ile Val Cys Ala Ala Arg Val Phe
65 70 75 80
Gln Leu Thr Arg Glu Leu Gly Leu Lys Ser Asp Gly Gln Thr Ile Glu
85 90 95
Trp Leu Leu Arg Gln Ala Glu Pro Ser Ile Leu Ala Ala Thr Gly Ser
100 105 110
Gly Thr Thr Pro Ala Val Phe Ser Cys Ser Ser Ala Pro Ser Thr Ala
115 120 125
Ser Ser Ser Phe Leu Leu Gly Lys Arg Pro Arg Gln Glu Asp His Glu
130 135 140
Ala Pro Thr Phe Trp Glu Ala Leu Gln Gln Gln Pro Arg Pro Ala Val
145 150 155 160
Ser Ser Trp Gly Ala Leu Val Ser Pro Ser Gln Glu Ala Gln Ala Tyr
165 170 175
Ala Ser Ser Val Ala Gln Val His His Leu Asn Leu Leu Ser Ala Leu
180 185 190
Ser Gly Ala Ala Thr Arg Arg Pro Ala Gln Glu Glu Ser Arg
195 200 205
<210>13
<211>810
<212>DNA
<213>毛果杨(Populus trichocarpa)
<400>13
atgtcagaca acagtggcgc tgtaaataac ggatctttga tagacccaca aagaaaccag 60
ccacctggag gtggtggtgg aggaggagga gttactaatg gagctttagt tgctaaaaaa 120
ccaccttcta aagaccgtca tagcaaagtt gatggtcgtg gaaggaggat tcggatgccg 180
atcatttgtg ctgctagagt ttttcagttg actcgtgaat tgggtcataa atccgatgga 240
caaactattg aatggctact tcgtcaagct gagccttcga ttattgctgc tactggtact 300
ggaactactc ctgctagttt ttctactgtt tctgtttcgg ttcgtgctgg tgggaattca 360
aattctatat cttctctttc gagcaataat attcatccgg ttggttcttt ggagcacaag 420
cctttgttag gcccagcgcc gtttatactt gggaaaagga tgcggccgga ggaggatggt 480
aatggtggtg ggaaggacga tgatggggtt cctgtgggcc ccacaattgg gtcattaatg 540
ggccccacag caacagccgg gcctggtggg ttctgggctt tacctgccag gcccgatttt 600
tgggggtttg ctgctgctgc accgccggaa atggttgttc agccgactgc ggtgcagcag 660
tcttcactgt ttatgcacca gcatgctgcg gctatgggag aagcgtcggc ggcaagagtg 720
gggaattatc ttcctggtca tttgaatttg ctggcttctt tgtcgagtgg acatggaagt 780
tcaggaagga gagaggatga tcaccgttga 810
<210>14
<211>269
<212>PRT
<213>毛果杨
<400>14
Met Ser Asp Asn Ser Gly Ala Val Asn Asn Gly Ser Leu Ile Asp Pro
1 5 10 15
Gln Arg Asn Gln Pro Pro Gly Gly Gly Gly Gly Gly Gly Gly Val Thr
20 25 30
Asn Gly Ala Leu Val Ala Lys Lys Pro Pro Ser Lys Asp Arg His Ser
35 40 45
Lys Val Asp Gly Arg Gly Arg Arg Ile Arg Met Pro Ile Ile Cys Ala
50 55 60
Ala Arg Val Phe Gln Leu Thr Arg Glu Leu Gly His Lys Ser Asp Gly
65 70 75 80
Gln Thr Ile Glu Trp Leu Leu Arg Gln Ala Glu Pro Ser Ile Ile Ala
85 90 95
Ala Thr Gly Thr Gly Thr Thr Pro Ala Ser Phe Ser Thr Val Ser Val
100 105 110
Ser Val Arg Ala Gly Gly Asn Ser Asn Ser Ile Ser Ser Leu Ser Ser
115 120 125
Asn Asn Ile His Pro Val Gly Ser Leu Glu His Lys Pro Leu Leu Gly
130 135 140
Pro Ala Pro Phe Ile Leu Gly Lys Arg Met Arg Pro Glu Glu Asp Gly
145 150 155 160
Asn Gly Gly Gly Lys Asp Asp Asp Gly Val Pro Val Gly Pro Thr Ile
165 170 175
Gly Ser Leu Met Gly Pro Thr Ala Thr Ala Gly Pro Gly Gly Phe Trp
180 185 190
Ala Leu Pro Ala Arg Pro Asp Phe Trp Gly Phe Ala Ala Ala Ala Pro
195 200 205
Pro Glu Met Val Val Gln Pro Thr Ala Val Gln Gln Ser Ser Leu Phe
210 215 220
Met His Gln His Ala Ala Ala Met Gly Glu Ala Ser Ala Ala Arg Val
225 230 235 240
Gly Asn Tyr Leu Pro Gly His Leu Asn Leu Leu Ala Ser Leu Ser Ser
245 250 255
Gly His Gly Ser Ser Gly Arg Arg Glu Asp Asp His Arg
260 265
<210>15
<211>594
<212>DNA
<213>番茄(Solanum lycopersicum)
<400>15
atgccaattg tttgtgctgc tagggttttt cagctaactc gtgaactagg tcataaatcc 60
gatggacaaa caattgaatg gctgttgcgt caagctgaac cttcgattat tgctgctact 120
ggtactggta ctatacctgc tagtttctct actgtttcag tttcgttacg aaattcgatt 180
tcttctgtta ctgcttctgc tccggttgat cataagttgc cgtcgccgtc gccgtcgttg 240
ttacatccgt tgatttctcc ggcgccgttt ttgcttggta agcgtttacg ttctgaggat 300
gatgataatg gaagtggaga taaggatgtg gttcctagtg ctggattttg ggcagttcct 360
gctaggccgg attttggaca agtttggagt ttcgctgcac cgtcgccgga gatttcacat 420
tccgctgcag cagcgatgaa tacgcagcca tcgaggtttc ttcagcagca gatggaagag 480
gcatcagctg gtagagttgg gaattacttt cctatagctc aagggcatct gaatttgcta 540
gcttctctat ctggttcggg ttctggaccg agaagggacg acgatggaca atga 594
<210>16
<211>197
<212>PRT
<213>番茄
<400>16
Met Pro Ile Val Cys Ala Ala Arg Val Phe Gln Leu Thr Arg Glu Leu
1 5 10 15
Gly His Lys Ser Asp Gly Gln Thr Ile Glu Trp Leu Leu Arg Gln Ala
20 25 30
Glu Pro Ser Ile Ile Ala Ala Thr Gly Thr Gly Thr Ile Pro Ala Ser
35 40 45
Phe Ser Thr Val Ser Val Ser Leu Arg Asn Ser Ile Ser Ser Val Thr
50 55 60
Ala Ser Ala Pro Val Asp His Lys Leu Pro Ser Pro Ser Pro Ser Leu
65 70 75 80
Leu His Pro Leu Ile Ser Pro Ala Pro Phe Leu Leu Gly Lys Arg Leu
85 90 95
Arg Ser Glu Asp Asp Asp Asn Gly Ser Gly Asp Lys Asp Val Val Pro
100 105 110
Ser Ala Gly Phe Trp Ala Val Pro Ala Arg Pro Asp Phe Gly Gln Val
115 120 125
Trp Ser Phe Ala Ala Pro Ser Pro Glu Ile Ser His Ser Ala Ala Ala
130 135 140
Ala Met Asn Thr Gln Pro Ser Arg Phe Leu Gln Gln Gln Met Glu Glu
145 150 155 160
Ala Ser Ala Gly Arg Val Gly Asn Tyr Phe Pro Ile Ala Gln Gly His
165 170 175
Leu Asn Leu Leu Ala Ser Leu Ser Gly Ser Gly Ser Gly Pro Arg Arg
180 185 190
Asp Asp Asp Gly Gln
195
<210>17
<211>606
<212>DNA
<213>葡萄(Vitis vinifera)
<400>17
atgtctacct cagatggagc ggctaacggc gttactaacg gcgctattgt tgactcccag 60
aggcagcagc agcccggtaa cggggccttg gcggtaaaga agccgccggc caaggaccgc 120
cacagcaaag tcgacggtcg gggcaggcgt atccggatgc cgatcatctg cgccgccagg 180
gttttccagt tgacgcggga gctggggcac aaatctgatg gtcaaactat cgagtggctg 240
ctgcggcagg cggagccgtc gatcatagcg gccacaggga cggggaccac tcctgcgagt 300
ttttcgagcc cgtcgcagcc gctgctcagc ccgacgccgt ttattctcgg gaagcgtcta 360
cggggcgacg acgacgtcga cggcggggcc aaagatgagg tgtggagctt cgccgctgcc 420
gcagctgctg ccccgccaga gatggtgccg acgatctccg ggaggttcat gcagcagccg 480
atgggagagg cgtcggcggc tagagttggg aattatcttc cgatcgcgca gggacatctg 540
aatttgctgg cgtcgttgtc tggagctccg gcatcgtcgg ggaggagaga cgacgatcca 600
cgttga 606
<210>18
<211>201
<212>PRT
<213>葡萄
<400>18
Met Ser Thr Ser Asp Gly Ala Ala Asn Gly Val Thr Asn Gly Ala Ile
1 5 10 15
Val Asp Ser Gln Arg Gln Gln Gln Pro Gly Asn Gly Ala Leu Ala Val
20 25 30
Lys Lys Pro Pro Ala Lys Asp Arg His Ser Lys Val Asp Gly Arg Gly
35 40 45
Arg Arg Ile Arg Met Pro Ile Ile Cys Ala Ala Arg Val Phe Gln Leu
50 55 60
Thr Arg Glu Leu Gly His Lys Ser Asp Gly Gln Thr Ile Glu Trp Leu
65 70 75 80
Leu Arg Gln Ala Glu Pro Ser Ile Ile Ala Ala Thr Gly Thr Gly Thr
85 90 95
Thr Pro Ala Ser Phe Ser Ser Pro Ser Gln Pro Leu Leu Ser Pro Thr
100 105 110
Pro Phe Ile Leu Gly Lys Arg Leu Arg Gly Asp Asp Asp Val Asp Gly
115 120 125
Gly Ala Lys Asp Glu Val Trp Ser Phe Ala Ala Ala Ala Ala Ala Ala
130 135 140
Pro Pro Glu Met Val Pro Thr Ile Ser Gly Arg Phe Met Gln Gln Pro
145 150 155 160
Met Gly Glu Ala Ser Ala Ala Arg Val Gly Asn Tyr Leu Pro Ile Ala
165 170 175
Gln Gly His Leu Asn Leu Leu Ala Ser Leu Ser Gly Ala Pro Ala Ser
180 185 190
Ser Gly Arg Arg Asp Asp Asp Pro Arg
195 200
<210>19
<211>1197
<212>DNA
<213>金鱼草(Antirrhinum majus)
<220>
<221>misc_feature
<222>(968)..(968)
<223>n是a、c、g或t
<400>19
atggaaggag gcgatgatca tcaccaccac caccaccacc acaaccaaag ccgcccaaac 60
ttccctttcc aactactcga aaaaaagaca accaataatc caccctcctc cgccctcgcc 120
atctccgccg acatcgcttc aaaccctagc accaccacct ccaccaccac tctaactgcc 180
gcctcagccg aaacatcgaa gaaaccacct ccaaagcgta catcaaccaa agaccgccac 240
accaaagtcg acggccgagg gcgacgtatc cgaatgcccg ccctatgcgc tgctagggtt 300
tttcagctca ctcgagaact cggccacaaa tctgacggcg agacaatcga gtggctgcta 360
caacaggcag aaccctccgt catagcagcc acaggtaccg gcacaattcc cgctaacttc 420
acctctctaa acatttcatt acgtagctcc ggttcaagca tgtctgtccc atctcaacta 480
agatcctcct acttcaatcc ttcgaattat acgatgatgt cgcagccgcg gcgagggttt 540
tcgtttcccg gtatagggtt gtcatcatca gagagttctt cttcaggtac cctattgaat 600
ttccaacagg cagcggcaaa tttaaaccct agcttgatgc atcaagctaa gcaagaaatg 660
cgagaaaaca gtaacaataa taataattca ttggagtcgg aggaggaaaa tatggggcgg 720
aagaggaggg cggagcagga gctgcagcaa caccagcatc accagattgg taactacttg 780
gtccagtcga gtaccggacc aatggcggcc agccacgcgt ccataccagc taacttctgg 840
acgctggcga attccagtaa taataacaac aacaacaaca acaataataa taatcaaggg 900
atggggagcg atccgatatg gacgtttcca tcggttaata ttaacaatag cgctgctgct 960
gctgctgntt tgtacagagg aactgtttct agtgggctgc attttatgaa ttttccggca 1020
ccggtggcgt tgttgcctag tcaacaaatt ggtggaaatg ttttgggaga agggcagttg 1080
ggtaatttca tgaaccctta taggaatatc ggtggaggcg gaggagcgtc ggagtcacag 1140
gcgagtggct cacacggtgg tgatgatcgg catgatagca caagtcacca ttcttga 1197
<210>20
<211>398
<212>PRT
<213>金鱼草
<220>
<221>misc_feature
<222>(323)..(323)
<223>Xaa可以是任意天然氨基酸
<400>20
Met Glu Gly Gly Asp Asp His His His His His His His His Asn Gln
1 5 10 15
Ser Arg Pro Asn Phe Pro Phe Gln Leu Leu Glu Lys Lys Thr Thr Asn
20 25 30
Asn Pro Pro Ser Ser Ala Leu Ala Ile Ser Ala Asp Ile Ala Ser Asn
35 40 45
Pro Ser Thr Thr Thr Ser Thr Thr Thr Leu Thr Ala Ala Ser Ala Glu
50 55 60
Thr Ser Lys Lys Pro Pro Pro Lys Arg Thr Ser Thr Lys Asp Arg His
65 70 75 80
Thr Lys Val Asp Gly Arg Gly Arg Arg Ile Arg Met Pro Ala Leu Cys
85 90 95
Ala Ala Arg Val Phe Gln Leu Thr Arg Glu Leu Gly His Lys Ser Asp
100 105 110
Gly Glu Thr Ile Glu Trp Leu Leu Gln Gln Ala Glu Pro Ser Val Ile
115 120 125
Ala Ala Thr Gly Thr Gly Thr Ile Pro Ala Asn Phe Thr Ser Leu Asn
130 135 140
Ile Ser Leu Arg Ser Ser Gly Ser Ser Met Ser Val Pro Ser Gln Leu
145 150 155 160
Arg Ser Ser Tyr Phe Asn Pro Ser Asn Tyr Thr Met Met Ser Gln Pro
165 170 175
Arg Arg Gly Phe Ser Phe Pro Gly Ile Gly Leu Ser Ser Ser Glu Ser
180 185 190
Ser Ser Ser Gly Thr Leu Leu Asn Phe Gln Gln Ala Ala Ala Asn Leu
195 200 205
Asn Pro Ser Leu Met His Gln Ala Lys Gln Glu Met Arg Glu Asn Ser
210 215 220
Asn Asn Asn Asn Asn Ser Leu Glu Ser Glu Glu Glu Asn Met Gly Arg
225 230 235 240
Lys Arg Arg Ala Glu Gln Glu Leu Gln Gln His Gln His His Gln Ile
245 250 255
Gly Asn Tyr Leu Val Gln Ser Ser Thr Gly Pro Met Ala Ala Ser His
260 265 270
Ala Ser Ile Pro Ala Asn Phe Trp Thr Leu Ala Asn Ser Ser Asn Asn
275 280 285
Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Gln Gly Met Gly Ser Asp
290 295 300
Pro Ile Trp Thr Phe Pro Ser Val Asn Ile Asn Asn Ser Ala Ala Ala
305 310 315 320
Ala Ala Xaa Leu Tyr Arg Gly Thr Val Ser Ser Gly Leu His Phe Met
325 330 335
Asn Phe Pro Ala Pro Val Ala Leu Leu Pro Ser Gln Gln Ile Gly Gly
340 345 350
Asn Val Leu Gly Glu Gly Gln Leu Gly Asn Phe Met Asn Pro Tyr Arg
355 360 365
Asn Ile Gly Gly Gly Gly Gly Ala Ser Glu Ser Gln Ala Ser Gly Ser
370 375 380
His Gly Gly Asp Asp Arg His Asp Ser Thr Ser His His Ser
385 390 395
<210>21
<211>1470
<212>DNA
<213>拟南芥
<400>21
atgcaaaagc caacatcaag tatcttaaat gtcataatgg acggtggaga cagcgtcgga 60
ggaggaggag gagatgatca ccaccgtcac ctccaccatc accaccgccc tactttccct 120
tttcaactac tcggaaaaca cgaccccgac gacaaccatc aacaacaacc ttccccttcc 180
tcctcctcct ctctattttc tctccatcaa caccaacaat tatctcaatc gcaacctcaa 240
tcgcaatcgc aaaagtcaca accgcagaca acgcaaaaag agttattaca aacgcaagag 300
gaatctgcgg tggtggcagc taaaaagcca ccgttgaaac gagcgtcgac gaaagaccga 360
cacacgaaag tagacggaag agggaggaga ataaggatgc cggcgttatg tgcagctagg 420
gtttttcagc taacgcgaga gctaggtcat aaatccgacg gtgagacaat agagtggctt 480
cttcaacaag ctgaaccatc tgtaatcgcc gccaccggaa ccggaacaat cccggcgaat 540
ttcacttctt taaacatctc tctccgttct tcaggctctt ccatgtctct tccttctcat 600
ttccgctccg ccgcttccac ttttagccct aataacatat tttctccggc gatgcttcaa 660
caacaacaac aacaacaacg tggtggtggt gttgggtttc atcatcccca tctacaggga 720
cgtgcaccta cgtcgtcttt gtttcctggt attgataact tcacaccaac gacgtcgttt 780
ttgaactttc ataatccaac aaagcaagaa ggagatcaag attctgaaga gttaaactcg 840
gagaagaaaa gaagaatcca aacgacgtcg gatttgcatc aacaacaaca acaacaccaa 900
catgatcaaa tcggaggata tacacttcaa tctagcaaca gtggatctac agccaccgca 960
gccgccgcgc aacaaatacc gggaaatttc tggatggttg cggcggctgc ggctgcaggt 1020
ggtggtggtg gtaataacaa ccaaacaggt ggtcttatga cagcttctat tggtactggt 1080
ggcggcggtg gagagcctgt ttggacgttt ccttccatta acacggcagc ggcagcgtta 1140
tatagaagtg gcgtttcggg cgttccaagc ggcgcggttt ctagcggttt acattttatg 1200
aatttcgcag cgccaatggc atttcttact ggacaacaac agctagcaac aactagtaat 1260
catgagatta atgaagatag taataataat gaaggaggaa gaagtgacgg tggtggtgat 1320
catcataata cacagagaca tcatcatcat caacaacaac atcatcataa tattctctcc 1380
ggcttgaacc agtacggacg gcaagtttcc ggcgactctc aagctagtgg atcacttgga 1440
ggtggtgatg aggaggatca gcaagattag 1470
<210>22
<211>489
<212>PRT
<213>拟南芥
<400>22
Met Gin Lys Pro Thr Ser Ser Ile Leu Asn Val Ile Met Asp Gly Gly
1 5 10 15
Asp Ser Val Gly Gly Gly Gly Gly Asp Asp His His Arg His Leu His
20 25 30
His His His Arg Pro Thr Phe Pro Phe Gln Leu Leu Gly Lys His Asp
35 40 45
Pro Asp Asp Asn His Gln Gln Gln Pro Ser Pro Ser Ser Ser Ser Ser
50 55 60
Leu Phe Ser Leu His Gln His Gln Gln Leu Ser Gln Ser Gln Pro Gln
65 70 75 80
Ser Gln Ser Gln Lys Ser Gln Pro Gln Thr Thr Gln Lys Glu Leu Leu
85 90 95
Gln Thr Gln Glu Glu Ser Ala Val Val Ala Ala Lys Lys Pro Pro Leu
100 105 110
Lys Arg Ala Ser Thr Lys Asp Arg His Thr Lys Val Asp Gly Arg Gly
115 120 125
Arg Arg Ile Arg Met Pro Ala Leu Cys Ala Ala Arg Val Phe Gln Leu
130 135 140
Thr Arg Glu Leu G1y His Lys Ser Asp Gly Glu Thr Ile Glu Trp Leu
145 150 155 160
Leu Gln Gln Ala Glu Pro Ser Val Ile Ala Ala Thr Gly Thr Gly Thr
165 170 175
Ile Pro Ala Asn Phe Thr Ser Leu Asn Ile Ser Leu Arg Ser Ser Gly
180 185 190
Ser Ser Met Ser Leu Pro Ser His Phe Arg Ser Ala Ala Ser Thr Phe
195 200 205
Ser Pro Asn Asn Ile Phe Ser Pro Ala Met Leu Gln Gln Gln Gln Gln
210 215 220
Gln Gln Arg Gly Gly Gly Val Gly Phe His His Pro His Leu Gln Gly
225 230 235 240
Arg Ala Pro Thr Ser Ser Leu Phe Pro Gly Ile Asp Asn Phe Thr Pro
245 250 255
Thr Thr Ser Phe Leu Asn Phe His Asn Pro Thr Lys Gln Glu Gly Asp
260 265 270
Gln Asp Ser Glu Glu Leu Asn Ser Glu Lys Lys Arg Arg Ile Gln Thr
275 280 285
Thr Ser Asp Leu His Gln Gln Gln Gln Gln His Gln His Asp Gln Ile
290 295 300
Gly Gly Tyr Thr Leu Gln Ser Ser Asn Ser Gly Ser Thr Ala Thr Ala
305 310 315 320
Ala Ala Ala Gln Gln Ile Pro Gly Asn Phe Trp Met Val Ala Ala Ala
325 330 335
Ala Ala Ala Gly Gly Gly Gly Gly Asn Asn Asn Gln Thr Gly Gly Leu
340 345 350
Met Thr Ala Ser Ile Gly Thr Gly Gly Gly Gly Gly Glu Pro Val Trp
355 360 365
Thr Phe Pro Ser Ile Asn Thr Ala Ala Ala Ala Leu Tyr Arg Ser Gly
370 375 380
Val Ser Gly Val Pro Ser Gly Ala Val Ser Ser Gly Leu His Phe Met
385 390 395 400
Asn Phe Ala Ala Pro Met Ala Phe Leu Thr Gly Gln Gln Gln Leu Ala
405 410 415
Thr Thr Ser Asn His Glu Ile Asn Glu Asp Ser Asn Asn Asn Glu Gly
420 425 430
Gly Arg Ser Asp Gly Gly Gly Asp His His Asn Thr Gln Arg His His
435 440 445
His His Gln Gln Gln His His His Asn Ile Leu Ser Gly Leu Asn Gln
450 455 460
Tyr Gly Arg Gln Val Ser Gly Asp Ser Gln Ala Ser Gly Ser Leu Gly
465 470 475 480
Gly Gly Asp Glu Glu Asp Gln Gln Asp
485
<210>23
<211>978
<212>DNA
<213>拟南芥
<400>23
atggatccgg atccggatca taaccatcga cccaacttcc ctctccagct tcttgattct 60
tctacctcct cctcttccac ttccttagcc atcatctcta ctacttccga acctaactcc 120
gaacctaaga agcctcctcc taaacgaacc tctactaaag accgacacac caaagtcgaa 180
ggccgaggcc gtcggatccg tatgcctgcc atgtgtgctg cacgtgtctt tcagctcaca 240
cgtgagcttg gtcacaaatc cgacggtgaa actattgagt ggctactcca acaagcagaa 300
ccagcggtta tagccgctac agggactgga accattccgg ctaacttcac ttctttaaac 360
atctcacttc gtagctcaag atcttctctc tctgctgctc atcttcgtac aactcctagt 420
agctattact ttcattcacc acatcagtcc atgactcatc atcttcaaca tcagcatcag 480
gttcgtccca agaacgagtc acattcttcg tcttcttctt cttcacagct tttagatcac 540
aaccaaatgg gtaactatct agtacaatca actgctggat ctttacctac gagtcagagt 600
cctgcaacgg caccgttttg gagtagtggt gacaacacac agaatctttg ggcttttaat 660
attaatcctc atcattccgg tgttgtcgcc ggagatgttt acaaccccaa cagtggtggt 720
agtggcggcg gtagtggagt tcatttgatg aattttgcag ctcctattgc tttgttttct 780
ggacagcctt tggcttctgg ttatggagga ggaggaggtg gcggtggaga acatagccat 840
tatggagttt tagcggcgtt gaatgctgct taccgaccgg tggcggagac ggggaaccat 900
aacaacaacc agcaaaaccg tgacggagat catcatcaca accatcaaga agatggaagc 960
accagtcatc attcctag 978
<210>24
<211>325
<212>PRT
<213>拟南芥
<400>24
Met Asp Pro Asp Pro Asp His Asn His Arg Pro Asn Phe Pro Leu Gln
1 5 10 15
Leu Leu Asp Ser Ser Thr Ser Ser Ser Ser Thr Ser Leu Ala Ile Ile
20 25 30
Ser Thr Thr Ser Glu Pro Asn Ser Glu Pro Lys Lys Pro Pro Pro Lys
35 40 45
Arg Thr Ser Thr Lys Asp Arg His Thr Lys Val Glu Gly Arg Gly Arg
50 55 60
Arg Ile Arg Met Pro Ala Met Cys Ala Ala Arg Val Phe Gln Leu Thr
65 70 75 80
Arg Glu Leu Gly His Lys Ser Asp Gly Glu Thr Ile Glu Trp Leu Leu
85 90 95
Gln Gln Ala Glu Pro Ala Val Ile Ala Ala Thr Gly Thr Gly Thr Ile
100 105 110
Pro Ala Asn Phe Thr Ser Leu Asn Ile Ser Leu Arg Ser Ser Arg Ser
115 120 125
Ser Leu Ser Ala Ala His Leu Arg Thr Thr Pro Ser Ser Tyr Tyr Phe
130 135 140
His Ser Pro His Gln Ser Met Thr His His Leu Gln His Gln His Gln
145 150 155 160
Val Arg Pro Lys Asn Glu Ser His Ser Ser Ser Ser Ser Ser Ser Gln
165 170 175
Leu Leu Asp His Asn Gln Met Gly Asn Tyr Leu Val Gln Ser Thr Ala
180 185 190
Gly Ser Leu Pro Thr Ser Gln Ser Pro Ala Thr Ala Pro Phe Trp Ser
195 200 205
Ser Gly Asp Asn Thr Gln Asn Leu Trp Ala Phe Asn Ile Asn Pro His
210 215 220
His Ser Gly Val Val Ala Gly Asp Val Tyr Asn Pro Asn Ser Gly Gly
225 230 235 240
Ser Gly Gly Gly Ser Gly Val His Leu Met Asn Phe Ala Ala Pro Ile
245 250 255
Ala Leu Phe Ser Gly Gln Pro Leu Ala Ser Gly Tyr Gly Gly Gly Gly
260 265 270
Gly Gly Gly Gly Glu His Ser His Tyr Gly Val Leu Ala Ala Leu Asn
275 280 285
Ala Ala Tyr Arg Pro Val Ala Glu Thr Gly Asn His Asn Asn Asn Gln
290 295 300
Gln Asn Arg Asp Gly Asp His His His Asn His Gln Glu Asp Gly Ser
305 310 315 320
Thr Ser His His Ser
325
<210>25
<211>945
<212>DNA
<213>棉花(Gossipumm hirsutum)
<400>25
atggaaggag gaggcagtga tgatcatcac caccaccacc acagccacca ccatcaccac 60
caacaaccac tccaccatca tcatcatcac caccaccgcc ctactttccc attccaatta 120
ctagagaaga aagaagaaga taaccaacct tgttcctcct cttcttccct tcctttccct 180
tccctaccgg tttcatcttc ctcggccgat caacacacct cgactcgttc catttcaacc 240
caccagatct cgccggaaac ttccaagact ccaccgccta aacgtacttc gactaaagac 300
cgacacacca aggtagatgg acgtggtcgt aggatccgta tgccggctct ttgtgcagct 360
agggtttttc agcttactcg cgagttgggt cataaataca atggtgaaac gatcgagtgg 420
ttgctacaac aagctgagcc ggcggtgatc gccgctacgg ggactggtac catccctgca 480
aacttcactt ccctcaatat ctcactccgt agctccggtt caagcatgtc ggttccttct 540
cagcttcgtt cctcgggttt tagctctaac ttttctatgc aacaacgtag aagcttgttc 600
cccggaatcg ggctcgaaac gacgccgacg ccgacgacgt tccttaactt ccaatctagt 660
agtacttcgt tgggaatatc ggaagaagcc gaagaaagta gtttaggaag gaaacgaaga 720
cccgaacaag atttatcttc acaacatcat cagaatgggg agttacttgt tgcatcaagc 780
accgtacatt ccggcgagtc accacgctca agttccggta acttttggat ggtaactaat 840
tctaataatc aagctatgag tggtggtgac cctatatgga cattccttca gttaataaca 900
gtggtttgta taggggaact atgtctagtg ggttgcattt catga 945
<210>26
<211>314
<212>PRT
<213>棉花
<400>26
Met Glu Gly Gly Gly Ser Asp Asp His His His His His His Ser His
1 5 10 15
His His His His Gln Gln Pro Leu His His His His His His His His
20 25 30
Arg Pro Thr Phe Pro Phe Gln Leu Leu Glu Lys Lys Glu Glu Asp Asn
35 40 45
Gln Pro Cys Ser Ser Ser Ser Ser Leu Pro Phe Pro Ser Leu Pro Val
50 55 60
Ser Ser Ser Ser Ala Asp Gln His Thr Ser Thr Arg Ser Ile Ser Thr
65 70 75 80
His Gln Ile Ser Pro Glu Thr Ser Lys Thr Pro Pro Pro Lys Arg Thr
85 90 95
Ser Thr Lys Asp Arg His Thr Lys Val Asp Gly Arg Gly Arg Arg Ile
100 105 110
Arg Met Pro Ala Leu Cys Ala Ala Arg Val Phe Gln Leu Thr Arg Glu
115 120 125
Leu Gly His Lys Tyr Asn Gly Glu Thr Ile Glu Trp Leu Leu Gln Gln
130 135 140
Ala Glu Pro Ala Val Ile Ala Ala Thr Gly Thr Gly Thr Ile Pro Ala
145 150 155 160
Asn Phe Thr Ser Leu Asn Ile Ser Leu Arg Ser Ser Gly Ser Ser Met
165 170 175
Ser Val Pro Ser Gln Leu Arg Ser Ser Gly Phe Ser Ser Asn Phe Ser
180 185 190
Met Gln Gln Arg Arg Ser Leu Phe Pro Gly Ile Gly Leu Glu Thr Thr
195 200 205
Pro Thr Pro Thr Thr Phe Leu Asn Phe Gln Ser Ser Ser Thr Ser Leu
210 215 220
Gly Ile Ser Glu Glu Ala Glu Glu Ser Ser Leu Gly Arg Lys Arg Arg
225 230 235 240
Pro Glu Gln Asp Leu Ser Ser Gln His His Gln Asn Gly Glu Leu Leu
245 250 255
Val Ala Ser Ser Thr Val His Ser Gly Glu Ser Pro Arg Ser Ser Ser
260 265 270
Gly Asn Phe Trp Met Val Thr Asn Ser Asn Asn Gln Ala Met Ser Gly
275 280 285
Gly Asp Pro Ile Trp Thr Phe Leu Gln Leu Ile Thr Val Val Cys Ile
290 295 300
Gly Glu Leu Cys Leu Val Gly Cys Ile Ser
305 310
<210>27
<211>1164
<212>DNA
<213>稻
<400>27
atggatgtca ccggagacgg cggaggagga gggcaacggc ccaatttccc cctgcagctc 60
ctcgggaaga aggaggagca gacgtgctcg acgtcgcaga ctgccggggc gggcggcggc 120
ggcgtcgtgg gcgcgaatgg gtcggcggcg gcggcgccgc cgaagcggac gtcgacgaag 180
gaccggcaca cgaaggtgga cgggcggggg cggcgcatcc ggatgccggc gatctgcgcc 240
gcgcgggtgt tccagctgac gcgggagctc gggcacaaga ccgacggcga gaccatcgag 300
tggctgctgc agcaggcgga gccggcggtg atcgcggcga ccgggacggg caccatcccg 360
gccaacttca cctccctcaa catctccctc cgctcctccg gctcgtcgct ctccatccct 420
tctcacctcc gccttgccgg cttggctggc cctcgcttcg gcggcggcgc gcgggcggcg 480
gacgcgtggg accgcgtcgt cggcctcggg ttcggcggtg cggccgacgc cccgtcctcc 540
gccacctcct cctcctcgtc gccgcttctg ctgagcttcc actccggtag cgtcggcctt 600
gacgtgtcgc cgccgtcggc gtcgacctcc ccggccgccg ccgacctctc ccggaagcgg 660
cggtgggagc aagaaatgca gcagcagcag cagtaccagc agcagatggc cgggtacacg 720
cagagccaaa ttcctgcggg cacggtgtgg atggtgccga gcagcaacgc gcaggccgcc 780
ggtggcggcg ctccgccggg aggcggcggc gagtcgattt ggacgttccc gcagtcaggg 840
agcggcggcg gcggcggcgc ggcgacagtg taccgtggcg tgccaagcgg actacatttc 900
atgaacttcc cggcgacacc aatggcgctg ctccccggcg ggcagcagct cggcctcgcc 960
ggcgccggcg ggggtggcga ggggcacccg gggatcctcg ccgcgctcaa tgcctaccgc 1020
gcacaggccg cgcagccgga cgccggcgcg gcggcgcaga atggagcgca aggctcaagt 1080
cagcatcgtc agcatcagca tcacggcggc ggcggcggcg gcggcgacga gcggcatgag 1140
agcatgagcg ccagcgactc gtag 1164
<210>28
<211>387
<212>PRT
<213>稻
<400>28
Met Asp Val Thr Gly Asp Gly Gly Gly Gly Gly Gln Arg Pro Asn Phe
1 5 10 15
Pro Leu Gln Leu Leu Gly Lys Lys Glu Glu Gln Thr Cys Ser Thr Ser
20 25 30
Gln Thr Ala Gly Ala Gly Gly Gly Gly Val Val Gly Ala Asn Gly Ser
35 40 45
Ala Ala Ala Ala Pro Pro Lys Arg Thr Ser Thr Lys Asp Arg His Thr
50 55 60
Lys Val Asp Gly Arg Gly Arg Arg Ile Arg Met Pro Ala Ile Cys Ala
65 70 75 80
Ala Arg Val Phe Gln Leu Thr Arg Glu Leu Gly His Lys Thr Asp Gly
85 90 95
Glu Thr Ile Glu Trp Leu Leu Gln Gln Ala Glu Pro Ala Val Ile Ala
100 105 110
Ala Thr Gly Thr Gly Thr Ile Pro Ala Asn Phe Thr Ser Leu Asn Ile
115 120 125
Ser Leu Arg Ser Ser Gly Ser Ser Leu Ser Ile Pro Ser His Leu Arg
130 135 140
Leu Ala Gly Leu Ala Gly Pro Arg Phe Gly Gly Gly Ala Arg Ala Ala
145 150 155 160
Asp Ala Trp Asp Arg Val Val Gly Leu Gly Phe Gly Gly Ala Ala Asp
165 170 175
Ala Pro Ser Ser Ala Thr Ser Ser Ser Ser Ser Pro Leu Leu Leu Ser
180 185 190
Phe His Ser Gly Ser Val Gly Leu Asp Val Ser Pro Pro Ser Ala Ser
195 200 205
Thr Ser Pro Ala Ala Ala Asp Leu Ser Arg Lys Arg Arg Trp Glu Gln
210 215 220
Glu Met Gln Gln Gln Gln Gln Tyr Gln Gln Gln Met Ala Gly Tyr Thr
225 230 235 240
Gln Ser Gln Ile Pro Ala Gly Thr Val Trp Met Val Pro Ser Ser Asn
245 250 255
Ala Gln Ala Ala Gly Gly Gly Ala Pro Pro Gly Gly Gly Gly Glu Ser
260 265 270
Ile Trp Thr Phe Pro Gln Ser Gly Ser Gly Gly Gly Gly Gly Ala Ala
275 280 285
Thr Val Tyr Arg Gly Val Pro Ser Gly Leu His Phe Met Asn Phe Pro
290 295 300
Ala Thr Pro Met Ala Leu Leu Pro Gly Gly Gln Gln Leu Gly Leu Ala
305 310 315 320
Gly Ala Gly Gly Gly Gly Glu Gly His Pro Gly Ile Leu Ala Ala Leu
325 330 335
Asn Ala Tyr Arg Ala Gln Ala Ala Gln Pro Asp Ala Gly Ala Ala Ala
340 345 350
Gln Asn Gly Ala Gln Gly Ser Ser Gln His Arg Gln His Gln His His
355 360 365
Gly Gly Gly Gly Gly Gly Gly Asp Glu Arg His Glu Ser Met Ser Ala
370 375 380
Ser Asp Ser
385
<210>29
<211>1233
<212>DNA
<213>稻
<400>29
atgaccatgg acgtcgccgg agacgccgga ggtggccgcc gcccaaactt ccccttgcag 60
cttcttgaga agaaggagga gcagccgtgc tccagctcgg ctgcaggagg tggtactggg 120
ccatcatcgg cgggtggcaa cgggaacaat ggatcgggac cgggcggtgc cggaggggag 180
atgcagctgc ggaaggcggc gccgaagcgg agctccacca aggaccggca caccaaggtg 240
gaagggaggg ggcggcgcat ccggatgccg gcgctgtgcg cggcgagggt gttccagctg 300
acgcgggagc tggggcacaa gacggacggc gagaccatcg agtggctgct gcagcaggcg 360
gagccggcgg tgatcgcggc caccggcacc ggcaccatcc cggccaactt cacctcgctc 420
aatatctccc tccgctcctc cggatcgtcg ctgtccgccc cggcgcacct ccgcgcgtta 480
cctagccccg ccgccgccgc ccggttcgga tcccgtgcgg acgcgtggga ccgggttgtc 540
agcctcgggt tcccgtccga gggccccgcc tcgtcgtcct cgtcgccgtc cccgcttctg 600
ttgaacttcc actcgggcag cgtcggtctc gatgtgcaac cttcgccttc cgccgctgcc 660
gccgccgccg acctctccag gaagcggcgc tgggagcagg aaatgcagca gcagcaacag 720
caacaacagc agcagcagca gcagcagcag caacagtacc agcagcagat ggccgggtac 780
acgcagagcc aaatgccggg caccgtgtgg atggtgccga gcaacagcac gcaaggaggt 840
gggccgtccg gtggtggcgg tggcggcgga ggaggcagcg gggagtccat ttggacgttc 900
ccgcagatga gcagcgccgc cgcggccgcc gccgtgtacc gggggagcgt tccgagcggg 960
ctacatttca tgaacttccc tgcaccgatg gcgctcctac cgggacagca gctggggctc 1020
ggcccagttg gaggcggcgg tggcggtgga ggaggcgggg aggggcacat ggggatcctt 1080
gccgccctca atgcttaccg gacacaggcg gcgacggatg ccgccggtca gcaaggcgga 1140
ggaggaggtg gaggaggatc tagccagcag caacacggag gcggcggcgg cggcggcgag 1200
cggcatcaga gcataagcac cagcgactcg tag 1233
<210>30
<211>410
<212>PRT
<213>稻
<400>30
Met Thr Met Asp Val Ala Gly Asp Ala Gly Gly Gly Arg Arg Pro Asn
1 5 10 15
Phe Pro Leu Gln Leu Leu Glu Lys Lys Glu Glu Gln Pro Cys Ser Ser
20 25 30
Ser Ala Ala Gly Gly Gly Thr Gly Pro Ser Ser Ala Gly Gly Asn Gly
35 40 45
Asn Asn Gly Ser Gly Pro Gly Gly Ala Gly Gly Glu Met Gln Leu Arg
50 55 60
Lys Ala Ala Pro Lys Arg Ser Ser Thr Lys Asp Arg His Thr Lys Val
65 70 75 80
Glu Gly Arg Gly Arg Arg Ile Arg Met Pro Ala Leu Cys Ala Ala Arg
85 90 95
Val Phe Gln Leu Thr Arg Glu Leu Gly His Lys Thr Asp Gly Glu Thr
100 105 110
Ile Glu Trp Leu Leu Gln Gln Ala Glu Pro Ala Val Ile Ala Ala Thr
115 120 125
Gly Thr Gly Thr Ile Pro Ala Asn Phe Thr Ser Leu Asn Ile Ser Leu
130 135 140
Arg Ser Ser Gly Ser Ser Leu Ser Ala Pro Ala His Leu Arg Ala Leu
145 150 155 160
Pro Ser Pro Ala Ala Ala Ala Arg Phe Gly Ser Arg Ala Asp Ala Trp
165 170 175
Asp Arg Val Val Ser Leu Gly Phe Pro Ser Glu Gly Pro Ala Ser Ser
180 185 190
Ser Ser Ser Pro Ser Pro Leu Leu Leu Asn Phe His Ser Gly Ser Val
195 200 205
Gly Leu Asp Val Gln Pro Ser Pro Ser Ala Ala Ala Ala Ala Ala Asp
210 215 220
Leu Ser Arg Lys Arg Arg Trp Glu Gln Glu Met Gln Gln Gln Gln Gln
225 230 235 240
Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Tyr Gln Gln Gln
245 250 255
Met Ala Gly Tyr Thr Gln Ser Gln Met Pro Gly Thr Val Trp Met Val
260 265 270
Pro Ser Asn Ser Thr Gln Gly Gly Gly Pro Ser Gly Gly Gly Gly Gly
275 280 285
Gly Gly Gly Gly Ser Gly Glu Ser Ile Trp Thr Phe Pro Gln Met Ser
290 295 300
Ser Ala Ala Ala Ala Ala Ala Val Tyr Arg Gly Ser Val Pro Ser Gly
305 310 315 320
Leu His Phe Met Asn Phe Pro Ala Pro Met Ala Leu Leu Pro Gly Gln
325 330 335
Gln Leu Gly Leu Gly Pro Val Gly Gly Gly Gly Gly Gly Gly Gly Gly
340 345 350
Gly Glu Gly His Met Gly Ile Leu Ala Ala Leu Asn Ala Tyr Arg Thr
355 360 365
Gln Ala Ala Thr Asp Ala Ala Gly Gln Gln Gly Gly Gly Gly Gly Gly
370 375 380
Gly Gly Ser Ser Gln Gln Gln His Gly Gly Gly Gly Gly Gly Gly Glu
385 390 395 400
Arg His Gln Ser Ile Ser Thr Ser Asp Ser
405 410
<210>31
<211>1188
<212>DNA
<213>毛果杨
<400>31
atggaaggag gtgatgatca gttccaccat cagcaccatc accagcatca ccaccgccca 60
accttcccat tccaattgct ggaaaagaaa gaagacgagc cttgctcttc ctcttctcct 120
tacccttctc ttcccatctc cactaccgaa cctaacagca acagcaacag tacgaacaca 180
aatcactcta cctcgaatct ccaaattgag gttgctgaac catcaaagaa accccctcca 240
aaacgaacat caacaaaaga cagacacacg aaggtggatg gacgtggtcg tagaattcgc 300
atgccagctc tctgcgctgc tagggttttt cagctcactc gagagcttgg ccacaaatca 360
gatggtgaaa ccatcgagtg gttacttcaa caagctgaac cagcagtgat cgctgccaca 420
ggcacgggca caatccctgc taatttcact tcactcaaca tatccttgcg tagctctggt 480
tcaagtatgt caattccttc tcacctaaga tcagggagtt tcaatccaaa cttttcactg 540
cagcaacgta gtcggagttt gttccccgga attgggctag aaacctcaca accaccgaca 600
ttcctaagtt tccaatctag taacaatctg aatgcagtgc tgcaagcgaa gcaagaattg 660
cgagacaatt cttcactgga attatcaacg gagacggaag agagtttaag caggaaacga 720
aggtccgagc aagatttatc accatcgcaa cagcatcaga tggggaatta cttgttacaa 780
tccagtgcag gggcaatccc agctagtcat ggtcaaattc cagcaaattt ttggatgctg 840
gctaattcca ataacaatca agtcatgagc ggagacccta tatggacatt tccttctgtc 900
aacaacagta gtgtgtacag aggcagcatg tcaagtgggt tgcattttat gaattttcct 960
gctcctgtta ccttattgcc tagccagcaa ctggggtcta ctagcattgg tggtgccagt 1020
ggtggtaata gtgggataag tgaagggcat ttgaacatgc tagctggatt gaatccctac 1080
cgaccttcag gtctatcaga atcacaggcg agtgggtctc actcacatca tggtggcggt 1140
ggtggaggaa gcgatgatcg gcatgataca acgagtcatc actcatag 1188
<210>32
<211>395
<212>PRT
<213>毛果杨
<400>32
Met Glu Gly Gly Asp Asp Gln Phe His His Gln His His His Gln His
1 5 10 15
His His Arg Pro Thr Phe Pro Phe Gln Leu Leu Glu Lys Lys Glu Asp
20 25 30
Glu Pro Cys Ser Ser Ser Ser Pro Tyr Pro Ser Leu Pro Ile Ser Thr
35 40 45
Thr Glu Pro Asn Ser Asn Ser Asn Ser Thr Asn Thr Asn His Ser Thr
50 55 60
Ser Asn Leu Gln Ile Glu Val Ala Glu Pro Ser Lys Lys Pro Pro Pro
65 70 75 80
Lys Arg Thr Ser Thr Lys Asp Arg His Thr Lys Val Asp Gly Arg Gly
85 90 95
Arg Arg Ile Arg Met Pro Ala Leu Cys Ala Ala Arg Val Phe Gln Leu
100 105 110
Thr Arg Glu Leu Gly His Lys Ser Asp Gly Glu Thr Ile Glu Trp Leu
115 120 125
Leu Gln Gln Ala Glu Pro Ala Val Ile Ala Ala Thr Gly Thr Gly Thr
130 135 140
Ile Pro Ala Asn Phe Thr Ser Leu Asn Ile Ser Leu Arg Ser Ser Gly
145 150 155 160
Ser Ser Met Ser Ile Pro Ser His Leu Arg Ser Gly Ser Phe Asn Pro
165 170 175
Asn Phe Ser Leu Gln Gln Arg Ser Arg Ser Leu Phe Pro Gly Ile Gly
180 185 190
Leu Glu Thr Ser Gln Pro Pro Thr Phe Leu Ser Phe Gln Ser Ser Asn
195 200 205
Asn Leu Asn Ala Val Leu Gln Ala Lys Gln Glu Leu Arg Asp Asn Ser
210 215 220
Ser Leu Glu Leu Ser Thr Glu Thr Glu Glu Ser Leu Ser Arg Lys Arg
225 230 235 240
Arg Ser Glu Gln Asp Leu Ser Pro Ser Gln Gln His Gln Met Gly Asn
245 250 255
Tyr Leu Leu Gln Ser Ser Ala Gly Ala Ile Pro Ala Ser His Gly Gln
260 265 270
Ile Pro Ala Asn Phe Trp Met Leu Ala Asn Ser Asn Asn Asn Gln Val
275 280 285
Met Ser Gly Asp Pro Ile Trp Thr Phe Pro Ser Val Asn Asn Ser Ser
290 295 300
Val Tyr Arg Gly Ser Met Ser Ser Gly Leu His Phe Met Asn Phe Pro
305 310 315 320
Ala Pro Val Thr Leu Leu Pro Ser Gln Gln Leu Gly Ser Thr Ser Ile
325 330 335
Gly Gly Ala Ser Gly Gly Asn Ser Gly Ile Ser Glu Gly His Leu Asn
340 345 350
Met Leu Ala Gly Leu Asn Pro Tyr Arg Pro Ser Gly Leu Ser Glu Ser
355 360 365
Gln Ala Ser Gly Ser His Ser His His Gly Gly Gly Gly Gly Gly Ser
370 375 380
Asp Asp Arg His Asp Thr Thr Ser His His Ser
385 390 395
<210>33
<211>960
<212>DNA
<213>马铃薯(Solanum demissum)
<400>33
atgactacct ctagtagtga tgcaccttgt gttgatcata accaaaaatc aaaaaatcaa 60
gacaagaaac aacaagcaaa aaaaccaaca acaacaaaag atagacacac aaaggttgat 120
ggtcgtggtc gaagaattcg tatgccagca acatgtgcag ctagggtttt tcaactcact 180
agagaattag gtcacaaatc tgatggtgaa actattgaat ggctcttaca acaagctgaa 240
cctgctgtta ttgctgctac tggtactggt actattccag ctaattattc atcacttaat 300
atttctttaa gaagttctag acatcatagt gctagtaact acctagcaca taataatatt 360
aataatttcg gtcatgttta tcatgaccga aattatttta atggggttgg tttattttcg 420
tccgaaaata attcattttt ccctagtggg aatttgaaca tgttacaagc gaaggaggag 480
ttatgtgatg acgatgataa tgataatgat aataataata atactggacg aattaagcgg 540
aggtcagaag ataaagactt acaacacaat aattaccata tgagtaatat gttacaatct 600
agtacttatg gttcaattcc agcaagtcat caaattgggc atattccagc aacaacgtta 660
tatatgatga caaataataa taataatact agtcatcatg accctaataa ttgttctatg 720
tgggcatctc ctagtaatag taatattgag aattcaaata ttagaggagg gttattgaat 780
tttatgaatt ttcatcaacc aattttgggg acacgtggcg gtggcggcgg tgggacggcg 840
gcagaagggc aatgggggat gttgacggcg gcgatgaact cgtatagaca aagtggtggt 900
catgctagtg gttcttctaa tcagcataat ggaggagatg atcatcaaca tcattcttga 960
<210>34
<211>319
<212>PRT
<213>马铃薯
<400>34
Met Thr Thr Ser Ser Ser Asp Ala Pro Cys Val Asp His Asn Gln Lys
1 5 10 15
Ser Lys Asn Gln Asp Lys Lys Gln Gln Ala Lys Lys Pro Thr Thr Thr
20 25 30
Lys Asp Arg His Thr Lys Val Asp Gly Arg Gly Arg Arg Ile Arg Met
35 40 45
Pro Ala Thr Cys Ala Ala Arg Val Phe Gln Leu Thr Arg Glu Leu Gly
50 55 60
His Lys Ser Asp Gly Glu Thr Ile Glu Trp Leu Leu Gln Gln Ala Glu
65 70 75 80
Pro Ala Val Ile Ala Ala Thr Gly Thr Gly Thr Ile Pro Ala Asn Tyr
85 90 95
Ser Ser Leu Asn Ile Ser Leu Arg Ser Ser Arg His His Ser Ala Ser
100 105 110
Asn Tyr Leu Ala His Asn Asn Ile Asn Asn Phe Gly His Val Tyr His
115 120 125
Asp Arg Asn Tyr Phe Asn Gly Val Gly Leu Phe Ser Ser Glu Asn Asn
130 135 140
Ser Phe Phe Pro Ser Gly Asn Leu Asn Met Leu Gln Ala Lys Glu Glu
145 150 155 160
Leu Cys Asp Asp Asp Asp Asn Asp Asn Asp Asn Asn Asn Asn Thr Gly
165 170 175
Arg Ile Lys Arg Arg Ser Glu Asp Lys Asp Leu Gln His Asn Asn Tyr
180 185 190
His Met Ser Asn Met Leu Gln Ser Ser Thr Tyr Gly Ser Ile Pro Ala
195 200 205
Ser His Gln Ile Gly His Ile Pro Ala Thr Thr Leu Tyr Met Met Thr
210 215 220
Asn Asn Asn Asn Asn Thr Ser His His Asp Pro Asn Asn Cys Ser Met
225 230 235 240
Trp Ala Ser Pro Ser Asn Ser Asn Ile Glu Asn Ser Asn Ile Arg Gly
245 250 255
Gly Leu Leu Asn Phe Met Asn Phe His Gln Pro Ile Leu Gly Thr Arg
260 265 270
Gly Gly Gly Gly Gly Gly Thr Ala Ala Glu Gly Gln Trp Gly Met Leu
275 280 285
Thr Ala Ala Met Asn Ser Tyr Arg Gln Ser Gly Gly His Ala Ser Gly
290 295 300
Ser Ser Asn Gln His Asn Gly Gly Asp Asp His Gln His His Ser
305 310 315
<210>35
<211>933
<212>DNA
<213>葡萄
<400>35
atgatcatgg agggagaaaa tggaatccgc cgccctaatt ttccattaca attactagag 60
aaaaacaagg atgaagtagc atccactgca tccaattcag gtccacagat ctcggagcct 120
cccaagaagc ctccacctaa aaggacctcc accaaggata ggcatactaa ggtcgatggg 180
agggggcgcc gcatccgcat gcctgccacc tgcgccgcta gggtgtttca gttgactagg 240
gaattaggcc acaagtccga cggcgagacc atcgagtggt tgttacaaca ggcggagccc 300
gcagtcatcg ctgccaccgg aaccggtaca attcccgcca attttacttc actgaatatc 360
tctcttcgga gctccggttc tagcatgtct gcaccttccc acctgaggac tagctactat 420
aatcccaact ttgccaggaa ccggaccgaa tgggaaagga atatcgatga atctttgcgc 480
agaattgcaa ccatagggag aaagaggcag ccagagcagg atccgtccca tagtcagatg 540
gggaactact tgttgcagtc gggttcgggg tctatcccgt ccactcatag tcagattccg 600
accacgtttt ggatggtgac taatccaagt aaccaagtga tgagtgggga tcccgtgtgg 660
acatttcctt ctgttaataa cagctccatg tatagaggga ccatgtcaag tgggctacat 720
tttatgaatt ttcctactcc aatggctctc ttgcctgggc agcagttggg ttcaggaatt 780
ggtggaagtg ggggcgtggc cgatggccac ttgggcatgt tagccgctct caatgcgttt 840
aggccgcttc ctggcaacgg ggcaccggac tcgcagcaga ctgcttcaca tccccacaac 900
ggaggagacc atagacatga tacaactagc tag 933
<210>36
<211>310
<212>PRT
<213>葡萄
<400>36
Met Ile Met Glu Gly Glu Asn Gly Ile Arg Arg Pro Asn Phe Pro Leu
1 5 10 15
Gln Leu Leu Glu Lys Asn Lys Asp Glu Val Ala Ser Thr Ala Ser Asn
20 25 30
Ser Gly Pro Gln Ile Ser Glu Pro Pro Lys Lys Pro Pro Pro Lys Arg
35 40 45
Thr Ser Thr Lys Asp Arg His Thr Lys Val Asp Gly Arg Gly Arg Arg
50 55 60
Ile Arg Met Pro Ala Thr Cys Ala Ala Arg Val Phe Gln Leu Thr Arg
65 70 75 80
Glu Leu Gly His Lys Ser Asp Gly Glu Thr Ile Glu Trp Leu Leu Gln
85 90 95
Gln Ala Glu Pro Ala Val Ile Ala Ala Thr Gly Thr Gly Thr Ile Pro
100 105 110
Ala Asn Phe Thr Ser Leu Asn Ile Ser Leu Arg Ser Ser Gly Ser Ser
115 120 125
Met Ser Ala Pro Ser His Leu Arg Thr Ser Tyr Tyr Asn Pro Asn Phe
130 135 140
Ala Arg Asn Arg Thr Glu Trp Glu Arg Asn Ile Asp Glu Ser Leu Arg
145 150 155 160
Arg Ile Ala Thr Ile Gly Arg Lys Arg Gln Pro Glu Gln Asp Pro Ser
165 170 175
His Ser Gln Met Gly Asn Tyr Leu Leu Gln Ser Gly Ser Gly Ser Ile
180 185 190
Pro Ser Thr His Ser Gln Ile Pro Thr Thr Phe Trp Met Val Thr Asn
195 200 205
Pro Ser Asn Gln Val Met Ser Gly Asp Pro Val Trp Thr Phe Pro Ser
210 215 220
Val Asn Asn Ser Ser Met Tyr Arg Gly Thr Met Ser Ser Gly Leu His
225 230 235 240
Phe Met Asn Phe Pro Thr Pro Met Ala Leu Leu Pro Gly Gln Gln Leu
245 250 255
Gly Ser Gly Ile Gly Gly Ser Gly Gly Val Ala Asp Gly His Leu Gly
260 265 270
Met Leu Ala Ala Leu Asn Ala Phe Arg Pro Leu Pro Gly Asn Gly Ala
275 280 285
Pro Asp Ser Gln Gln Thr Ala Ser His Pro His Asn Gly Gly Asp His
290 295 300
Arg His Asp Thr Thr Ser
305 310
<210>37
<211>1080
<212>DNA
<213>葡萄
<400>37
atgatggaag gaggtgatga gctccacagg ccaaattttc cattccaatt gctagaaaag 60
aaggaagaag aagcgtgctc cagctctgaa cccaacacca accgctctaa ttcatctcta 120
caaatcgcag cggagccttc caaaaagccg cctcccaaga ggacttccac caaggatcgg 180
cacaccaaag tcgacggacg tggccgccgc atccgcatgc ctgcactctg cgctgctagg 240
gtttttcagc taacgcgtga gctcggccat aaatccgacg gcgaaaccat cgagtggctg 300
cttcaacagg ccgagcctgc cgtgattgcc gccaccggaa ccggcacaat tcctgcaaat 360
tttacgtccc ttaacatctc cctccggagc tcgggctcaa gcatgtccgt cccatctcaa 420
ctaagatcca cttacttcaa tcccaacttc tcggtccctc aaagaagaag tctttttccg 480
gggctatcat cggagaattc ttccacactc gttaatttcc aatctaacaa tctaaattcc 540
atgtttcaag ccaagcaaga gttgagagac acttcactag acctatcgga gactgaggag 600
agcataggga ggaagaggcg gccggagcag gatttgtcgc aacaacaaat gggaagctac 660
ttgctgcagt cgagcaccgg ctccatcccg gccagccatg gtcagattcc ggcgaatttt 720
tggatgctca ctaattctaa taaccaagtc atgagtgctg accctatatg gccatttcct 780
tctgttaata acagtaactt atataggggc actatgtcca gtgggctaca ttttatgaat 840
tttccgactc ctgttgccct cttgcctagc cagcagttga gctccggcgg tggaggtggc 900
agtggtggtg gcggtggtgg cggcggcagt ggcatgggcg agggtcactt taacatgctg 960
gctggactaa acccttatcg gccggtgatg gggagtggag tgtcggaatc tcaggcaagt 1020
gggtctcagt cgcaccatgg aggtgatgat cgacatgatg caaccagtca ccactcatag 1080
<210>38
<211>359
<212>PRT
<213>葡萄
<400>38
Met Met Glu Gly Gly Asp Glu Leu His Arg Pro Asn Phe Pro Phe Gln
1 5 10 15
Leu Leu Glu Lys Lys Glu Glu Glu Ala Cys Ser Ser Ser Glu Pro Asn
20 25 30
Thr Asn Arg Ser Asn Ser Ser Leu Gln Ile Ala Ala Glu Pro Ser Lys
35 40 45
Lys Pro Pro Pro Lys Arg Thr Ser Thr Lys Asp Arg His Thr Lys Val
50 55 60
Asp Gly Arg Gly Arg Arg Ile Arg Met Pro Ala Leu Cys Ala Ala Arg
65 70 75 80
Val Phe Gln Leu Thr Arg Glu Leu Gly His Lys Ser Asp Gly Glu Thr
85 90 95
Ile Glu Trp Leu Leu Gln Gln Ala Glu Pro Ala Val Ile Ala Ala Thr
100 105 110
Gly Thr Gly Thr Ile Pro Ala Asn Phe Thr Ser Leu Asn Ile Ser Leu
115 120 125
Arg Ser Ser Gly Ser Ser Met Ser Val Pro Ser Gln Leu Arg Ser Thr
130 135 140
Tyr Phe Asn Pro Asn Phe Ser Val Pro Gln Arg Arg Ser Leu Phe Pro
145 150 155 160
Gly Leu Ser Ser Glu Asn Ser Ser Thr Leu Val Asn Phe Gln Ser Asn
165 170 175
Asn Leu Asn Ser Met Phe Gln Ala Lys Gln Glu Leu Arg Asp Thr Ser
180 185 190
Leu Asp Leu Ser Glu Thr Glu Glu Ser Ile Gly Arg Lys Arg Arg Pro
195 200 205
Glu Gln Asp Leu Ser Gln Gln Gln Met Gly Ser Tyr Leu Leu Gln Ser
210 215 220
Ser Thr Gly Ser Ile Pro Ala Ser His Gly Gln Ile Pro Ala Asn Phe
225 230 235 240
Trp Met Leu Thr Asn Ser Asn Asn Gln Val Met Ser Ala Asp Pro Ile
245 250 255
Trp Pro Phe Pro Ser Val Asn Asn Ser Asn Leu Tyr Arg Gly Thr Met
260 265 270
Ser Ser Gly Leu His Phe Met Asn Phe Pro Thr Pro Val Ala Leu Leu
275 280 285
Pro Ser Gln Gln Leu Ser Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly
290 295 300
Gly Gly Gly Gly Gly Ser Gly Met Gly Glu Gly His Phe Asn Met Leu
305 310 315 320
Ala Gly Leu Asn Pro Tyr Arg Pro Val Met Gly Ser Gly Val Ser Glu
325 330 335
Ser Gln Ala Ser Gly Ser Gln Ser His His Gly Gly Asp Asp Arg His
340 345 350
Asp Ala Thr Ser His His Ser
355
<210>39
<211>56
<212>DNA
<213>人工序列
<220>
<223>引物
<400>39
ggggacaagt ttgtacaaaa aagcaggctt aaacaatgtc taaccacaag gaaaca 56
<210>40
<211>51
<212>DNA
<213>人工序列
<220>
<223>引物
<400>40
ggggaccact ttgtacaaga aagctgggtg aataaagtac aaaacaccga a 51
<210>41
<211>56
<212>DNA
<213>人工序列
<220>
<223>引物
<400>41
ggggacaagt ttgtacaaaa aagcaggctt aaacaatgga attggaaggt gatcat 56
<210>42
<211>54
<212>DNA
<213>人工序列
<220>
<223>引物
<400>42
ggggaccact ttgtacaaga aagctgggtt cagatcatac acttctaatt gctt 54
<210>43
<211>2192
<212>DNA
<213>拟南芥
<400>43
atcggttcta gctgctaact ttttatttgt tgttgccttt cattgaaaaa aaaaaaactc 60
ataatacaaa gacgagccat ctctgattag atctacactt ctcctcgcac caaaattttg 120
ccagttttcg ttcctttgtt cataacattt tcgcggattc caaatggatt tcatgaaggt 180
cttcgatcaa actgttcgag agataaaaag ggaggtgaat ctgaaagttt tgaaggtccc 240
tgagatggag caaaaggtat tggatgctac agataatgag ccttggggtc cccatggtac 300
tgcattggca gagatagctc aggccacgaa gaaattctcg gagtgccaga tggttatgag 360
tgttttgtgg actagactaa gtgagacagg aaaagattgg cgatatgtct acaaggcatt 420
ggctgttatt gattatctga tttcgaatgg atcagaacga gcggttgatg agattattga 480
gcatacttac caaatatctt cactcacaag ttttgagtat gttgaaccaa atgggaaaga 540
tgtgggaatc aacgtgagaa agaaggcgga aaacatagtc gctcttttaa ataataaaga 600
gaaaatctca gaaatcagag acaaagcagt agcaaatcgt aacaagtatg ttggcctctc 660
atcaacggga ataacatata aatctggttc ctcagcttca tttggtggta gttttcaaag 720
tggttcaagc aattttgaca gctacaagga tagagattcc agagaagaca agaatgacta 780
cgagtctttt caaaagtcaa gacgtggtgt taaaactgag gagcaaagct acacttctaa 840
gaagagtttt tcacgctatg gcagcacgga ccatgacaat ctatccagtg gtaaaaagtc 900
acccgattct gccaaacata ggtcatatgt atctgcagct ccttcaaaca acgatgatga 960
ttttgatgat tttgatccac ggggaacttc cagtaataag ccatccacag gcagtgccaa 1020
ccaagtggac ctttttggag gagatctgat tggtgacttc ttggattctg ggccaacaga 1080
aacatcttcc accaacaaca acgagaactt tcaagaggcg gacttgtttg ctgatgcagc 1140
ttttgtatca gcctcggctc agggagctga atttgggtca cagacacaga aacaagttga 1200
tcttttctct gcgtctgaac cttctgtcac agtttcttca gctcctccga cggttgatct 1260
ctttgcatct tctgaatcag ttgtaagccc agaagccaag atatcaatac ctgagtctat 1320
ggctactccc aacattgttg acccatttgc tgcagttcct atggataatt ttgatggatc 1380
agatcctttt ggtgccttca cttcccactc ggcttcagtt tctacaggtc cacaagctcc 1440
aagtgtacat gggagtgcaa caaacaccac aagcccgctg tctttcgcag actcgaagcc 1500
acaacatttg caaaagaaag atcctttcca ggtgaaatca ggaatttggg cagattcgct 1560
gagccgtgga ctgattgatc tcaatataac tgctcccaaa aaagcttctc tagctgatgt 1620
gggtgttgtg ggggatctga gcaatgaaga tgggaacaaa gcatctgctg catcgtacta 1680
ctcaggctgg tccatgggtg caggatccgg gcttggtaaa actggcctat acagtacaca 1740
gcagcagcag caacaacaac aacaacaaca agcacctgat atctcagacg atttcttctc 1800
cagcctcagt aaccaaaggt accaatctgg tggctttaag cagtgaaatt gtctgtgatt 1860
tggtgttgag atgaaaaggc atatcaagtt tgattctaca ttccatactg gaaatgtaag 1920
tttttttaat gggtttgttg ttcttgcact tcattcatgg gacaatctgg tcggtgactg 1980
atgtgccatt gttgttgtgg cgtttacttt gggtttaaat gagagagaga agaattgtct 2040
ttatgttttt tttgataaga gagtactttt attattggtt taaagtttcg atttgttcat 2100
agaacaaaag tatatgtatc tcttcccact caacaaatat tttttttatc gcgtgtgatt 2160
cttaatccga aagagactcc aactttcttc tc 2192
<210>44
<211>560
<212>PRT
<213>拟南芥
<400>44
Met Asp Phe Met Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Met Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Asp Asn Glu Pro Trp Gly Pro His Gly Thr Ala Leu
35 40 45
Ala Glu Ile Ala Gln Ala Thr Lys Lys Phe Ser Glu Cys Gln Met Val
50 55 60
Met Ser Val Leu Trp Thr Arg Leu Ser Glu Thr Gly Lys Asp Trp Arg
65 70 75 80
Tyr Val Tyr Lys Ala Leu Ala Val Ile Asp Tyr Leu Ile Ser Asn Gly
85 90 95
Ser Glu Arg Ala Val Asp Glu Ile Ile Glu His Thr Tyr Gln Ile Ser
100 105 110
Ser Leu Thr Ser Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Ile Asn Val Arg Lys Lys Ala Glu Asn Ile Val Ala Leu Leu Asn Asn
130 135 140
Lys Glu Lys Ile Ser Glu Ile Arg Asp Lys Ala Val Ala Asn Arg Asn
145 150 155 160
Lys Tyr Val Gly Leu Ser Ser Thr Gly Ile Thr Tyr Lys Ser Gly Ser
165 170 175
Ser Ala Ser Phe Gly Gly Ser Phe Gln Ser Gly Ser Ser Asn Phe Asp
180 185 190
Ser Tyr Lys Asp Arg Asp Ser Arg Glu Asp Lys Asn Asp Tyr Glu Ser
195 200 205
Phe Gln Lys Ser Arg Arg Gly Val Lys Thr Glu Glu Gln Ser Tyr Thr
210 215 220
Ser Lys Lys Ser Phe Ser Arg Tyr Gly Ser Thr Asp His Asp Asn Leu
225 230 235 240
Ser Ser Gly Lys Lys Ser Pro Asp Ser Ala Lys His Arg Ser Tyr Val
245 250 255
Ser Ala Ala Pro Ser Asn Asn Asp Asp Asp Phe Asp Asp Phe Asp Pro
260 265 270
Arg Gly Thr Ser Ser Asn Lys Pro Ser Thr Gly Ser Ala Asn Gln Val
275 280 285
Asp Leu Phe Gly Gly Asp Leu Ile Gly Asp Phe Leu Asp Ser Gly Pro
290 295 300
Thr Glu Thr Ser Ser Thr Asn Asn Asn Glu Asn Phe Gln Glu Ala Asp
305 310 315 320
Leu Phe Ala Asp Ala Ala Phe Val Ser Ala Ser Ala Gln Gly Ala Glu
325 330 335
Phe Gly Ser Gln Thr Gln Lys Gln Val Asp Leu Phe Ser Ala Ser Glu
340 345 350
Pro Ser Val Thr Val Ser Ser Ala Pro Pro Thr Val Asp Leu Phe Ala
355 360 365
Ser Ser Glu Ser Val Val Ser Pro Glu Ala Lys Ile Ser Ile Pro Glu
370 375 380
Ser Met Ala Thr Pro Asn Ile Val Asp Pro Phe Ala Ala Val Pro Met
385 390 395 400
Asp Asn Phe Asp Gly Ser Asp Pro Phe Gly Ala Phe Thr Ser His Ser
405 410 415
Ala Ser Val Ser Thr Gly Pro Gln Ala Pro Ser Val His Gly Ser Ala
420 425 430
Thr Asn Thr Thr Ser Pro Leu Ser Phe Ala Asp Ser Lys Pro Gln His
435 440 445
Leu Gln Lys Lys Asp Pro Phe Gln Val Lys Ser Gly Ile Trp Ala Asp
450 455 460
Ser Leu Ser Arg Gly Leu Ile Asp Leu Asn Ile Thr Ala Pro Lys Lys
465 470 475 480
Ala Ser Leu Ala Asp Val Gly Val Val Gly Asp Leu Ser Asn Glu Asp
485 490 495
Gly Asn Lys Ala Ser Ala Ala Ser Tyr Tyr Ser Gly Trp Ser Met Gly
500 505 510
Ala Gly Ser Gly Leu Gly Lys Thr Gly Leu Tyr Ser Thr Gln Gln Gln
515 520 525
Gln Gln Gln Gln Gln Gln Gln Gln Ala Pro Asp Ile Ser Asp Asp Phe
530 535 540
Phe Ser Ser Leu Ser Asn Gln Arg Tyr Gln Ser Gly Gly Phe Lys Gln
545 550 555 560
<210>45
<211>2194
<212>DNA
<213>稻
<400>45
aatccgaaaa gtttctgcac cgttttcacc ccctaactaa caatataggg aacgtgtgct 60
aaatataaaa tgagacctta tatatgtagc gctgataact agaactatgc aagaaaaact 120
catccaccta ctttagtggc aatcgggcta aataaaaaag agtcgctaca ctagtttcgt 180
tttccttagt aattaagtgg gaaaatgaaa tcattattgc ttagaatata cgttcacatc 240
tctgtcatga agttaaatta ttcgaggtag ccataattgt catcaaactc ttcttgaata 300
aaaaaatctt tctagctgaa ctcaatgggt aaagagagag atttttttta aaaaaataga 360
atgaagatat tctgaacgta ttggcaaaga tttaaacata taattatata attttatagt 420
ttgtgcattc gtcatatcgc acatcattaa ggacatgtct tactccatcc caatttttat 480
ttagtaatta aagacaattg acttattttt attatttatc ttttttcgat tagatgcaag 540
gtacttacgc acacactttg tgctcatgtg catgtgtgag tgcacctcct caatacacgt 600
tcaactagca acacatctct aatatcactc gcctatttaa tacatttagg tagcaatatc 660
tgaattcaag cactccacca tcaccagacc acttttaata atatctaaaa tacaaaaaat 720
aattttacag aatagcatga aaagtatgaa acgaactatt taggtttttc acatacaaaa 780
aaaaaaagaa ttttgctcgt gcgcgagcgc caatctccca tattgggcac acaggcaaca 840
acagagtggc tgcccacaga acaacccaca aaaaacgatg atctaacgga ggacagcaag 900
tccgcgacaa ccttttaaca gcaggctttg cggccaggag agaggaggag aggcaaagaa 960
aaccaagcat cctccttctc ccatctataa attcctcccc ccttttcccc tctctatata 1020
ggaggcatcc aagccaagaa gagggagagc accaaggaca cgcgactagc agaagccgag 1080
cgaccgcctt ctcgatccat atcttccggt cgagttcttg gtcgatctct tccctcctcc 1140
acctcctcct cacagggtat gtgcctccct tcggttgttc ttggatttat tgttctaggt 1200
tgtgtagtac gggcgttgat gttaggaaag gggatctgta tctgtgatga ttcctgttct 1260
tggatttggg atagaggggt tcttgatgtt gcatgttatc ggttcggttt gattagtagt 1320
atggttttca atcgtctgga gagctctatg gaaatgaaat ggtttaggga tcggaatctt 1380
gcgattttgt gagtaccttt tgtttgaggt aaaatcagag caccggtgat tttgcttggt 1440
gtaataaagt acggttgttt ggtcctcgat tctggtagtg atgcttctcg atttgacgaa 1500
gctatccttt gtttattccc tattgaacaa aaataatcca actttgaaga cggtcccgtt 1560
gatgagattg aatgattgat tcttaagcct gtccaaaatt tcgcagctgg cttgtttaga 1620
tacagtagtc cccatcacga aattcatgga aacagttata atcctcagga acaggggatt 1680
ccctgttctt ccgatttgct ttagtcccag aatttttttt cccaaatatc ttaaaaagtc 1740
actttctggt tcagttcaat gaattgattg ctacaaataa tgcttttata gcgttatcct 1800
agctgtagtt cagttaatag gtaatacccc tatagtttag tcaggagaag aacttatccg 1860
atttctgatc tccattttta attatatgaa atgaactgta gcataagcag tattcatttg 1920
gattattttt tttattagct ctcacccctt cattattctg agctgaaagt ctggcatgaa 1980
ctgtcctcaa ttttgttttc aaattcacat cgattatcta tgcattatcc tcttgtatct 2040
acctgtagaa gtttcttttt ggttattcct tgactgcttg attacagaaa gaaatttatg 2100
aagctgtaat cgggatagtt atactgcttg ttcttatgat tcatttcctt tgtgcagttc 2160
ttggtgtagc ttgccacttt caccagcaaa gttc 2194
<210>46
<211>56
<212>DNA
<213>人工序列
<220>
<223>引物prm09481
<400>46
ggggacaagt ttgtacaaaa aagcaggctt aaacaatgga tttcatgaag gtcttc 56
<210>47
<211>50
<212>DNA
<213>人工序列
<220>
<223>引物prm09482
<400>47
ggggaccact ttgtacaaga aagctgggtt cacagacaat ttcactgctt 50
<210>48
<211>15
<212>PRT
<213>人工序列
<220>
<223>基序1
<220>
<221>变体
<222>(1)..(1)
<223>/取代=″Ile″
<220>
<221>变体
<222>(2)..(2)
<223>/取代=″Arg″
<220>
<221>变体
<222>(3)..(3)
<223>/取代=″Glu″
<220>
<221>变体
<222>(6)..(6)
<223>/取代=″Asp″/取代=″Asn″
<220>
<221>变体
<222>(7)..(7)
<223>/取代=″Asp″/取代=″Glu″/取代=″Ser″
<220>
<221>变体
<222>(9)..(9)
<223>/取代=″Ser″
<220>
<221>变体
<222>(15)..(15)
<223>/取代=″Ser″/取代=″Glu″
<400>48
Val Leu Asp Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Thr
1 5 10 15
<210>49
<211>16
<212>PRT
<213>人工序列
<220>
<223>基序2
<220>
<221>变体
<222>(2)..(2)
<223>/取代=″Glu″
<220>
<221>变体
<222>(3)..(3)
<223>/取代=″Phe″
<220>
<221>变体
<222>(4)..(4)
<223>/取代=″Leu″/取代=″Val″/取代=″Arg″/取代=
″Lys″
<220>
<221>变体
<222>(5)..(5)
<223>/取代=″Glu″
<220>
<221>变体
<222>(6)..(6)
<223>/取代=″Pro″/取代=″Ala″
<220>
<221>变体
<222>(7)..(7)
<223>/取代=″Gly″/取代=″Asn″/取代=″Gln″/取代=
″Arg″
<220>
<221>变体
<222>(9)..(9)
<223>/取代=″Lys″
<220>
<221>变体
<222>(11)..(11)
<223>/取代=″Val″/取代=″Ala″/取代=″His″/取代=
″Glu″
<220>
<221>变体
<222>(13)..(13)
<223>/取代=″Asn″/取代=″Leu″/取代=″Ile″/取代=
″Val″
<400>49
Phe Gln Tyr Ile Asp Ser Ser Gly Arg Asp Gln Gly Ser Asn Val Arg
1 5 10 15
<210>50
<211>12
<212>PRT
<213>人工序列
<220>
<223>基序3
<220>
<221>变体
<222>(1)..(1)
<223>/取代=″Ser″/取代=″Ala″/取代=″Gln″
<220>
<221>变体
<222>(2)..(2)
<223>/取代=″Ile″/取代=″Glu″/取代=″Ala″
<220>
<221>变体
<222>(4)..(4)
<223>/取代=″G1u″/取代=″Asp″/取代=″Asn″
<220>
<221>变体
<222>(7)..(7)
<223>/取代=″Leu″/取代=″Val″/取代=″Glu″
<220>
<221>变体
<222>(8)..(8)
<223>/取代=″Val″/取代=″Ser″/取代=″Arg″/取代=
″Lys″
<220>
<221>变体
<222>(9)..(9)
<223>/取代=″Thr″
<220>
<221>变体
<222>(10)..(10)
<223>/取代=″Ala″
<220>
<221>变体
<222>(11)..(11)
<223>/取代=″Glu″/取代=″Asn″/取代=″Gly″
<400>50
Glu Val Arg Gln Lys Ala Ala Ala Asn Arg Asp Lys
1 5 10
<210>51
<211>10
<212>PRT
<213>人工序列
<220>
<223>基序4
<220>
<221>变体
<222>(4)..(4)
<223>/取代=″Ser″
<220>
<221>变体
<222>(10)..(10)
<223>/取代=″Ile″
<400>51
Trp Ala Asp Thr Leu Ser Arg Gly Leu Val
1 5 10
<210>52
<211>9
<212>PRT
<213>人工序列
<220>
<223>基序5
<220>
<221>变体
<222>(2)..(2)
<223>/取代=″Ser″
<220>
<221>变体
<222>(4)..(4)
<223>/取代=″Val″
<220>
<221>变体
<222>(6)..(6)
<223>/取代=″Val″
<220>
<221>变体
<222>(7)..(7)
<223>/取代=″Val″
<220>
<221>变体
<222>(8)..(8)
<223>/取代=″Gly″
<220>
<221>变体
<222>(9)..(9)
<223>/取代=″Glu″/取代=″Pro″/取代=″Gly″
<400>52
Leu Ala Asp Ile Gly Ile Asp Phe Asp
1 5
<210>53
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序6a
<400>53
Gly Gly Tyr Gly
1
<210>54
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序6b
<400>54
Gly Ser Tyr Gly
1
<210>55
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序6c
<400>55
Gly Gly Tyr Asp
1
<210>56
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序7a
<400>56
Ser Ala Ala Ser
1
<210>57
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序7b
<400>57
Ser Ser Ala Ser
1
<210>58
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序7c
<400>58
Ser Ser Ala Pro
1
<210>59
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序7d
<400>59
Ser Ser Ala Thr
1
<210>60
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序8a
<400>60
Asp Glu Phe Asp
1
<210>61
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序8b
<400>61
Asp Phe Phe Asp
1
<210>62
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序8c
<400>62
Asp Asp Asp Phe
1
<210>63
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序8d
<400>63
Glu Asp Asp Phe
1
<210>64
<211>4
<212>PRT
<213>人工序列
<220>
<223>基序8e
<400>64
Asp Asp Phe Asp
1
<210>65
<211>1734
<212>DNA
<213>拟南芥
<400>65
atggatttca tgaaggtctt cgatcaaact gttcgagaga taaaaaggga ggtgaatctg 60
aaagttttga aggtccctga gatggagcaa aaggtattgg atgctacaga taatgagcct 120
tggggtcccc atggtactgc attggcagag atagctcagg ccacgaagaa attctcggag 180
tgccagatgg ttatgagtgt tttgtggact agactaagtg agacaggaaa agattggcga 240
tatgtctaca aggcattggc tgttattgat tatctgattt cgaatggatc agaacgagcg 300
gttgatgaga ttattgagca tacttaccaa atatcttcac tcacaagttt tgagtatgtt 360
gaaccaaatg ggaaagatgt gggaatcaac gtgagaaaga aggcggaaaa catagtcgct 420
cttttaaata ataaagagaa aatctcagaa atcagagaca aagcagtagc aaatcgtaac 480
aagtatgttg gcctctcatc aacgggaata acatataaat ctggttcctc agcttcattt 540
ggtggtagtt ttcaaagtgg ttcaagcaat tttgacagct acaaggatag agattccaga 600
gaagacaaga atgactacga gtcttttcaa aagtcaagac gtggtgttaa aactgaggag 660
caaagctaca cttctaagaa gagtttttca cgctatggca gcacggacca tgacaatcta 720
tccagtggta aaaagtcacc cgattctgcc aaacataggt catatgtatc tgcagctcct 780
tcaaacaacg atgatgattt tgatgatttt gatccacggg gaacttccag taatagtatg 840
tttctctcta cgactgctga gtttgcagat tgtttatcat tcattcggga gaaagttggc 900
agtgccaacc aagtggacct ttttggagga gatctgattg gtgacttctt ggattctggg 960
ccaacagaaa catcttccac caacaacaac gagaactttc aagaggcgga cttgtttgct 1020
gatgcagctt ttgtatcagc ctcggctcag ggagctgaat ttgggtcaca gacacagaaa 1080
caagttgatc ttttctctgc gtctgaacct tctgtcacag tttcttcagc tcctccgacg 1140
gttgatctct ttgcatcttc tgaatcagtt gtaagcccag aagccaagat atcaatacct 1200
gagtctatgg ctactcccaa cattgttgac ccatttgctg cagttcctat ggataatttt 1260
gatggatcag atccttttgg tgccttcact tcccactcgg cttcagtttc tacaggtcca 1320
caagctccaa gtgtacatgg gagtgcaaca aacaccacaa gcccgctgtc tttcgcagac 1380
tcgaagccac aacatttgca aaagaaagat cctttccagg tgaaatcagg aatttgggca 1440
gattcgctga gccgtggact gattgatctc aatataactg ctcccaaaaa agcttctcta 1500
gctgatgtgg gtgttgtggg ggatctgagc aatgaagatg ggaacaaagc atctgctgca 1560
tcgtactact caggctggtc catgggtgca ggatccgggc ttggtaaaac tggcctatac 1620
agtacacagc agcagcagca acaacaacaa caacaacaag cacctgatat ctcagacgat 1680
ttcttctcca gcctcagtaa ccaaaggtac caatctggtg gctttaagca gtga 1734
<210>66
<211>577
<212>PRT
<213>拟南芥
<400>66
Met Asp Phe Met Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Met Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Asp Asn Glu Pro Trp Gly Pro His Gly Thr Ala Leu
35 40 45
Ala Glu Ile Ala Gln Ala Thr Lys Lys Phe Ser Glu Cys Gln Met Val
50 55 60
Met Ser Val Leu Trp Thr Arg Leu Ser Glu Thr Gly Lys Asp Trp Arg
65 70 75 80
Tyr Val Tyr Lys Ala Leu Ala Val Ile Asp Tyr Leu Ile Ser Asn Gly
85 90 95
Ser Glu Arg Ala Val Asp Glu Ile Ile Glu His Thr Tyr Gln Ile Ser
100 105 110
Ser Leu Thr Ser Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Ile Asn Val Arg Lys Lys Ala Glu Asn Ile Val Ala Leu Leu Asn Asn
130 135 140
Lys Glu Lys Ile Ser Glu Ile Arg Asp Lys Ala Val Ala Asn Arg Asn
145 150 155 160
Lys Tyr Val Gly Leu Ser Ser Thr Gly Ile Thr Tyr Lys Ser Gly Ser
165 170 175
Ser Ala Ser Phe Gly Gly Ser Phe Gln Ser Gly Ser Ser Asn Phe Asp
180 185 190
Ser Tyr Lys Asp Arg Asp Ser Arg Glu Asp Lys Asn Asp Tyr Glu Ser
195 200 205
Phe Gln Lys Ser Arg Arg Gly Val Lys Thr Glu Glu Gln Ser Tyr Thr
210 215 220
Ser Lys Lys Ser Phe Ser Arg Tyr Gly Ser Thr Asp His Asp Asn Leu
225 230 235 240
Ser Ser Gly Lys Lys Ser Pro Asp Ser Ala Lys His Arg Ser Tyr Val
245 250 255
Ser Ala Ala Pro Ser Asn Asn Asp Asp Asp Phe Asp Asp Phe Asp Pro
260 265 270
Arg Gly Thr Ser Ser Asn Ser Met Phe Leu Ser Thr Thr Ala Glu Phe
275 280 285
Ala Asp Cys Leu Ser Phe Ile Arg Glu Lys Val Gly Ser Ala Asn Gln
290 295 300
Val Asp Leu Phe Gly Gly Asp Leu Ile Gly Asp Phe Leu Asp Ser Gly
305 310 315 320
Pro Thr Glu Thr Ser Ser Thr Asn Asn Asn Glu Asn Phe Gln Glu Ala
325 330 335
Asp Leu Phe Ala Asp Ala Ala Phe Val Ser Ala Ser Ala Gln Gly Ala
340 345 350
Glu Phe Gly Ser Gln Thr Gln Lys Gln Val Asp Leu Phe Ser Ala Ser
355 360 365
Glu Pro Ser Val Thr Val Ser Ser Ala Pro Pro Thr Val Asp Leu Phe
370 375 380
Ala Ser Ser Glu Ser Val Val Ser Pro Glu Ala Lys Ile Ser Ile Pro
385 390 395 400
Glu Ser Met Ala Thr Pro Asn Ile Val Asp Pro Phe Ala Ala Val Pro
405 410 415
Met Asp Asn Phe Asp Gly Ser Asp Pro Phe Gly Ala Phe Thr Ser His
420 425 430
Ser Ala Ser Val Ser Thr Gly Pro Gln Ala Pro Ser Val His Gly Ser
435 440 445
Ala Thr Asn Thr Thr Ser Pro Leu Ser Phe Ala Asp Ser Lys Pro Gln
450 455 460
His Leu Gln Lys Lys Asp Pro Phe Gln Val Lys Ser Gly Ile Trp Ala
465 470 475 480
Asp Ser Leu Ser Arg Gly Leu Ile Asp Leu Asn Ile Thr Ala Pro Lys
485 490 495
Lys Ala Ser Leu Ala Asp Val Gly Val Val Gly Asp Leu Ser Asn Glu
500 505 510
Asp Gly Asn Lys Ala Ser Ala Ala Ser Tyr Tyr Ser Gly Trp Ser Met
515 520 525
Gly Ala Gly Ser Gly Leu Gly Lys Thr Gly Leu Tyr Ser Thr Gln Gln
530 535 540
Gln Gln Gln Gln Gln Gln Gln Gln Gln Ala Pro Asp Ile Ser Asp Asp
545 550 555 560
Phe Phe Ser Ser Leu Ser Asn Gln Arg Tyr Gln Ser Gly Gly Phe Lys
565 570 575
Gln
<210>67
<211>1686
<212>DNA
<213>葡萄
<400>67
atggatttca tgaaagtctt cgatcagacg gtccgagaaa taaagaggga ggtgaatctg 60
aaggttttga aggttccaga gatcgagcag aaggtactgg atgcaaccga caatgaacct 120
tggggccctc atggctctgc cctggcagaa atagctcagg ccactaaaaa attcactgaa 180
tgtcagatgg ttatgaatgt tctgtggaca agattaagtg attctggccg agactggcgc 240
catgtctata agtcactggc tgttatagag tatttggtgg ctaatggatc tgaacgtgct 300
gttgatgata taattgaaca cactttccaa atctcatcac tttcaggatt tgagtatgtt 360
gaaccaaatg ggaaggatgt tggaatcaat gtgaggaaga aggctgaaac cattgtggct 420
cttttgaata acaaagaaaa aatacaagaa gttagaaata aagctgctgc aaaccgcgac 480
aagttctttg gactttcctc atctggagtg acatacaagt ctagttcagc cccatatggc 540
agcagcagct ttcagagtgc tgatcagcat ggaggtatga aaaatgattc atttagggat 600
agttataaag acagggatcg gtttgatgaa gaaaaggtcg acgaggatac ttctgctaag 660
tcacgtcaag gggttaccag tgagaatgaa gggaacacct ttaaaaaggg atctgcacgc 720
tatagcagca aggataaaga tactttgtca acaaaggcaa attactctga caagtacggg 780
tcaattccct cccacagttc aagtgtgcct tcaattaatg atgaagatga ttttgatgac 840
tttgatcccc ggggaacttc cagtaccaag accactgctg gaaacattaa ccaaccagac 900
ctgtttgggc aaagtttgat tggtgacctc atggatgcac cggcacctgt tcccacagaa 960
atgtctgcca tcaacagcaa ttctgcagag cctgatctgt ttgcagatgc aacttttgtt 1020
tcagcgccac ctcatgtaga agaaggttct agttctcagg ttgaggcgaa ggttgatcta 1080
tttgggtctc aacctgctgg tccttctgcg aatcctccag catttgactt ttttgctgcc 1140
cctgatccag ttgtgcaatc agaaaccaag tctcaaaaat ctgacacagc cagccccgac 1200
attgttgatc catttgctgc agttccacta aacagttttg atggatcaga tctttttggt 1260
tcattcactt cccataccaa ctctgcatct acagagccta cacaaagtcc tgcaaatgat 1320
ggcaacctta acaatttgaa tggaaaatcc tctgcagaat ttcaacctcc acccaagaag 1380
gatgctttcc aggttaaatc tggaatttgg gcagattcac tgagccgtgg actgattgat 1440
cttaatatat ccgcacccaa gaagataaat ctagcagatg ttggcattgt gggaggattg 1500
agtgatggat cggatgaaag ggagaaggga ccccaaactt cgttttccat gggacaagcc 1560
atgggtattg gctccggtct tggtaaatct gggtttacat ctccaccaac aggtggagtt 1620
ggtggagatg acatcttctc aagtctcggc agccagcaat accaattcgg aggcttcaag 1680
aaataa 1686
<210>68
<211>561
<212>PRT
<213>葡萄
<400>68
Met Asp Phe Met Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Asp Asn Glu Pro Trp Gly Pro His Gly Ser Ala Leu
35 40 45
Ala Glu Ile Ala Gln Ala Thr Lys Lys Phe Thr Glu Cys Gln Met Val
50 55 60
Met Asn Val Leu Trp Thr Arg Leu Ser Asp Ser Gly Arg Asp Trp Arg
65 70 75 80
His Val Tyr Lys Ser Leu Ala Val Ile Glu Tyr Leu Val Ala Asn Gly
85 90 95
Ser Glu Arg Ala Val Asp Asp Ile Ile Glu His Thr Phe Gln Ile Ser
100 105 110
Ser Leu Ser Gly Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Ile Asn Val Arg Lys Lys Ala Glu Thr Ile Val Ala Leu Leu Asn Asn
130 135 140
Lys Glu Lys Ile Gln Glu Val Arg Asn Lys Ala Ala Ala Asn Arg Asp
145 150 155 160
Lys Phe Phe Gly Leu Ser Ser Ser Gly Val Thr Tyr Lys Ser Ser Ser
165 170 175
Ala Pro Tyr Gly Ser Ser Ser Phe Gln Ser Ala Asp Gln His Gly Gly
180 185 190
Met Lys Asn Asp Ser Phe Arg Asp Ser Tyr Lys Asp Arg Asp Arg Phe
195 200 205
Asp Glu Glu Lys Val Asp Glu Asp Thr Ser Ala Lys Ser Arg Gln Gly
210 215 220
Val Thr Ser Glu Asn Glu Gly Asn Thr Phe Lys Lys Gly Ser Ala Arg
225 230 235 240
Tyr Ser Ser Lys Asp Lys Asp Thr Leu Ser Thr Lys Ala Asn Tyr Ser
245 250 255
Asp Lys Tyr Gly Ser Ile Pro Ser His Ser Ser Ser Val Pro Ser Ile
260 265 270
Asn Asp Glu Asp Asp Phe Asp Asp Phe Asp Pro Arg Gly Thr Ser Ser
275 280 285
Thr Lys Thr Thr Ala Gly Asn Ile Asn Gln Pro Asp Leu Phe Gly Gln
290 295 300
Ser Leu Ile Gly Asp Leu Met Asp Ala Pro Ala Pro Val Pro Thr Glu
305 310 315 320
Met Ser Ala Ile Asn Ser Asn Ser Ala Glu Pro Asp Leu Phe Ala Asp
325 330 335
Ala Thr Phe Val Ser Ala Pro Pro His Val Glu Glu Gly Ser Ser Ser
340 345 350
Gln Val Glu Ala Lys Val Asp Leu Phe Gly Ser Gln Pro Ala Gly Pro
355 360 365
Ser Ala Asn Pro Pro Ala Phe Asp Phe Phe Ala Ala Pro Asp Pro Val
370 375 380
Val Gln Ser Glu Thr Lys Ser Gln Lys Ser Asp Thr Ala Ser Pro Asp
385 390 395 400
Ile Val Asp Pro Phe Ala Ala Val Pro Leu Asn Ser Phe Asp Gly Ser
405 410 415
Asp Leu Phe Gly Ser Phe Thr Ser His Thr Asn Ser Ala Ser Thr Glu
420 425 430
Pro Thr Gln Ser Pro Ala Asn Asp Gly Asn Leu Asn Asn Leu Asn Gly
435 440 445
Lys Ser Ser Ala Glu Phe Gln Pro Pro Pro Lys Lys Asp Ala Phe Gln
450 455 460
Val Lys Ser Gly Ile Trp Ala Asp Ser Leu Ser Arg Gly Leu Ile Asp
465 470 475 480
Leu Asn Ile Ser Ala Pro Lys Lys Ile Asn Leu Ala Asp Val Gly Ile
485 490 495
Val Gly Gly Leu Ser Asp Gly Ser Asp Glu Arg Glu Lys Gly Pro Gln
500 505 510
Thr Ser Phe Ser Met Gly Gln Ala Met Gly Ile Gly Ser Gly Leu Gly
515 520 525
Lys Ser Gly Phe Thr Ser Pro Pro Thr Gly Gly Val Gly Gly Asp Asp
530 535 540
Ile Phe Ser Ser Leu Gly Ser Gln Gln Tyr Gln Phe Gly Gly Phe Lys
545 550 555 560
Lys
<210>69
<211>1785
<212>DNA
<213>稻
<400>69
atggatttcg tgaaggtttt cgatcaggcc gtgcgggaga taaagaggga ggtcaatttg 60
aaggtgctca aggttccaga gctcgaacag aaggtacttg atgcaacaag cgatgaacct 120
tggggccctc atggtactac tctttcagag ctatcacatg ccaccaagaa gttcgctgaa 180
tgtcagatgg taatgagtgt cctctggact aggctgtctg agcgaggctc gaaatggcgt 240
cacgtgtata aggctttgac aattattgag tacttaatag ccaatggttc tgagcgggca 300
gttgatgaca ttcttgatca ctattcaaag atctcggttc tttcaagttt tgaatatgtg 360
gaacctaatg gaaaagatgc tggaataaat gtgagaaaga aagtcgaaac tatattggga 420
ctcataaatg acaaggagaa aataaagtct gtaagagaga aagccgccag taatcgtgac 480
aagtatgttg ggctatcatc gacagggata acatataagt caagctctgc ttcatttggt 540
agcaactata gttctggtga acgttatggg agttttagtg gtacaaggga aggtgattca 600
tacggtgaca gttataggga taaagaacct gttaaatcct ctccaagtta tactggcagc 660
cagaaatctg gcagcaggat aaaaaaggat gtgaatagaa ggaatgagga ttcaccgagc 720
tccttgaagt ctaatgcaaa aggcaatgag gacgattttg atgattttga tcctcgtgga 780
tcttcttcaa atggtgcagc taatacaaac accagtggcg tggatctttt tgccccaaac 840
ttattggatg atttcatcga tgtgcctgca gcagcaactc atgaaacaaa cgactctgca 900
gacgctcagg ttgatctgtt tgctgatgca gatttccaat cagcaatacc aagtacagaa 960
acagctgcgg gttcagatgt ccagggcaat gtagaccttt ttgcggaaca gccagccttc 1020
acagcagcct ttccaccaca gacagggttt attccaccac caagttctgg gacatctgaa 1080
gcaaatactt ccacgtctaa gaacacaact cctgaacctt ttgatccttt tggtgctatt 1140
cctataaaca gctttgatgg atctgatcca tttggtgcct tcaactccga tgttggatca 1200
tctagtatac caccacccac gcagagttct gttggaaata tcagcacacc aagtcagaac 1260
cctcaagcag catctgactt tggtggcttt gtgtcgagca ctgtagaaac agcagctaag 1320
gacccctttg atttttcaag tagcaatctt gggaaaacac ctttggcaga tccaaaggct 1380
gatgcatccg attttggtgc ctttgtatcg cacagtgagg aagtagccaa ggatcctttt 1440
gatctttctt tgagtaccag ctctggaagg acaaaccaag cacctctggc ggctcccaag 1500
tcagatacca agaaagaaaa ttttcaggtc aagtctggca tatgggctga ctcattgagc 1560
cgtggactga ttgatctgaa cataactgga ccaaagaagg tgaacttagc cgacgttggg 1620
attgtcggtg gcctcgatga cgggtctgat gataaagctc tgccctcatg gaccatgggt 1680
gcaggaggtt ccagcctagg aatgtccgga attccttcat ctacacaaag tggtggcatt 1740
gagagcttag ctaactacaa caagtaccag tttggcttta aatga 1785
<210>70
<211>594
<212>PRT
<213>稻
<400>70
Met Asp Phe Val Lys Val Phe Asp Gln Ala Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Leu Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly Thr Thr Leu
35 40 45
Ser Glu Leu Ser His Ala Thr Lys Lys Phe Ala Glu Cys Gln Met Val
50 55 60
Met Ser Val Leu Trp Thr Arg Leu Ser Glu Arg Gly Ser Lys Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Thr Ile Ile Glu Tyr Leu Ile Ala Asn Gly
85 90 95
Ser Glu Arg Ala Val Asp Asp Ile Leu Asp His Tyr Ser Lys Ile Ser
100 105 110
Val Leu Ser Ser Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Ala Gly
115 120 125
Ile Asn Val Arg Lys Lys Val Glu Thr Ile Leu Gly Leu Ile Asn Asp
130 135 140
Lys Glu Lys Ile Lys Ser Val Arg Glu Lys Ala Ala Ser Asn Arg Asp
145 150 155 160
Lys Tyr Val Gly Leu Ser Ser Thr Gly Ile Thr Tyr Lys Ser Ser Ser
165 170 175
Ala Ser Phe Gly Ser Asn Tyr Ser Ser Gly Glu Arg Tyr Gly Ser Phe
180 185 190
Ser Gly Thr Arg Glu Gly Asp Ser Tyr Gly Asp Ser Tyr Arg Asp Lys
195 200 205
Glu Pro Val Lys Ser Ser Pro Ser Tyr Thr Gly Ser Gln Lys Ser Gly
210 215 220
Ser Arg Ile Lys Lys Asp Val Asn Arg Arg Asn Glu Asp Ser Pro Ser
225 230 235 240
Ser Leu Lys Ser Asn Ala Lys Gly Asn Glu Asp Asp Phe Asp Asp Phe
245 250 255
Asp Pro Arg Gly Ser Ser Ser Asn Gly Ala Ala Asn Thr Asn Thr Ser
260 265 270
Gly Val Asp Leu Phe Ala Pro Asn Leu Leu Asp Asp Phe Ile Asp Val
275 280 285
Pro Ala Ala Ala Thr His Glu Thr Asn Asp Ser Ala Asp Ala Gln Val
290 295 300
Asp Leu Phe Ala Asp Ala Asp Phe Gln Ser Ala Ile Pro Ser Thr Glu
305 310 315 320
Thr Ala Ala Gly Ser Asp Val Gln Gly Asn Val Asp Leu Phe Ala Glu
325 330 335
Gln Pro Ala Phe Thr Ala Ala Phe Pro Pro Gln Thr Gly Phe Ile Pro
340 345 350
Pro Pro Ser Ser Gly Thr Ser Glu Ala Asn Thr Ser Thr Ser Lys Asn
355 360 365
Thr Thr Pro Glu Pro Phe Asp Pro Phe Gly Ala Ile Pro Ile Asn Ser
370 375 380
Phe Asp Gly Ser Asp Pro Phe Gly Ala Phe Asn Ser Asp Val Gly Ser
385 390 395 400
Ser Ser Ile Pro Pro Pro Thr Gln Ser Ser Val Gly Asn Ile Ser Thr
405 410 415
Pro Ser Gln Asn Pro Gln Ala Ala Ser Asp Phe Gly Gly Phe Val Ser
420 425 430
Ser Thr Val Glu Thr Ala Ala Lys Asp Pro Phe Asp Phe Ser Ser Ser
435 440 445
Asn Leu Gly Lys Thr Pro Leu Ala Asp Pro Lys Ala Asp Ala Ser Asp
450 455 460
Phe Gly Ala Phe Val Ser His Ser Glu Glu Val Ala Lys Asp Pro Phe
465 470 475 480
Asp Leu Ser Leu Ser Thr Ser Ser Gly Arg Thr Asn Gln Ala Pro Leu
485 490 495
Ala Ala Pro Lys Ser Asp Thr Lys Lys Glu Asn Phe Gln Val Lys Ser
500 505 510
Gly Ile Trp Ala Asp Ser Leu Ser Arg Gly Leu Ile Asp Leu Asn Ile
515 520 525
Thr Gly Pro Lys Lys Val Asn Leu Ala Asp Val Gly Ile Val Gly Gly
530 535 540
Leu Asp Asp Gly Ser Asp Asp Lys Ala Leu Pro Ser Trp Thr Met Gly
545 550 555 560
Ala Gly Gly Ser Ser Leu Gly Met Ser Gly Ile Pro Ser Ser Thr Gln
565 570 575
Ser Gly Gly Ile Glu Ser Leu Ala Asn Tyr Asn Lys Tyr Gln Phe Gly
580 585 590
Phe Lys
<210>7l
<211>1887
<212>DNA
<213>稻
<400>7l
atggatttca ggaaggtgct cgaccagacc gtccgggaga taaggaggga ggtaaatctt 60
aaggtgctca aggtgccgga aatcgagcag aaggttcttg atgccaccag cgacgagccg 120
tgggggccgc atggttccga tttggcagat atcgccaggg ccaccaagag ctacggtgat 180
agcgaaatta taatgaatgt gttatggcaa cgcctgggga acacacttgc gaattggcgt 240
cacgtgtata aggcgttggc tgtgatcgag taccttctag ctaatggcac cgaacgtgca 300
gctgatggca ttgttgacaa tagctcacga attgcaaaac tcacaagatt tgagtatttg 360
gagcctaatg gaaaagatgt tgggctcaat gtgcgtaaga aggctgaagc tgttctagca 420
attttggatg acagggagaa gcttcaagag gtcagagaga aggctgccgt tactagagac 480
aagtattttg gcttatcatc aactggaata acgcacaaat cgagcgcagc atcatttggc 540
agtggcagct actcatctgg tagccactat gggagcacag gaggttcaag ggaggtggga 600
tcattcaagg atatacacac aggcacagaa tggaaaaaga acaagaagga aacagtgtca 660
aactacagca gcaatagaga agggtctaaa gaaattacta acagtgcaac cagttataag 720
tcaaaaaaga gtgaaaggca tggtagaaga aatcaaaatt ccttaacatt acactcgaag 780
ttatctgcaa atattagcac cacatctgaa gccccaagct caaagaaagg ggaaaatgag 840
gatgatgatg atttcaaccc acgaggattt tctacatcta ctggaacagg taccacaaga 900
tctaatcacc tggatctctt tggtccaagc ttgatggatg atcttgttga ttctactaca 960
tccacttcaa cagcaacgcc aaatgttagc acacctgctg tgccagaggt tgatttattt 1020
gcagatgcag ctttccaatc agccaatgct ccattggagg cagcaacggt ttctcacact 1080
caggacaaaa ttgatttgtt tgctggcaga ctgtcttctg ctgattcatt tacttcagac 1140
acagagttct cagtacgcgg tagtcctaac aagtcatcgg agaaaaaaat gtcttccgtt 1200
gtgcatcctt ctacttctgc ttttgatccc ttcaaacaat cttttgccac ctcatttcct 1260
tcagattcag agttctcagt tcatgatccg acaagcaaat cttctcaagg aaaaactccc 1320
acaccagaac attcaagtac agcagctttt gatccttttg ctgcaattcc actgaagagt 1380
tttgatggat ccgaatcttt tggaacattt tcttcgaaca cagcctcaaa cattactgaa 1440
ctgccacggg attcttctgg gggtcccaaa agttctgacc atggtccttt ggaggatgcc 1500
aattttgatg ccttcacttc acacttggga tcttccacaa caagtgcaac tgagtccatg 1560
aataagccca tcaaaaagct tgggcaggac tcgatgtcag catcaaaatc agttgcaaag 1620
aaagaaactt ttcaggtcaa atctggcata tgggctgact ctttgagccg aggattgatt 1680
gatttgaata taacttcgtc acaaaagaag gtcgatctct ccgatgttgg gattgtcgga 1740
ccgctgagcg gcggatctga ggataaaggc ccgggggcga cgatgggcac agcaccaggc 1800
cttgtcagtt ctagttttcc atctaaaaca gaaacatcca gtggaagcgg tcattttcag 1860
caccaacagt ttggaagctt taagtga 1887
<210>72
<211>628
<212>PRT
<213>稻
<400>72
Met Asp Phe Arg Lys Val Leu Asp Gln Thr Val Arg Glu Ile Arg Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly Ser Asp Leu
35 40 45
Ala Asp Ile Ala Arg Ala Thr Lys Ser Tyr Gly Asp Ser Glu Ile Ile
50 55 60
Met Asn Val Leu Trp Gln Arg Leu Gly Asn Thr Leu Ala Asn Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Ala Val Ile Glu Tyr Leu Leu Ala Asn Gly
85 90 95
Thr Glu Arg Ala Ala Asp Gly Ile Val Asp Asn Ser Ser Arg Ile Ala
100 105 110
Lys Leu Thr Arg Phe Glu Tyr Leu Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Leu Asn Val Arg Lys Lys Ala Glu Ala Val Leu Ala Ile Leu Asp Asp
130 135 140
Arg Glu Lys Leu Gln Glu Val Arg Glu Lys Ala Ala Val Thr Arg Asp
145 150 155 160
Lys Tyr Phe Gly Leu Ser Ser Thr Gly Ile Thr His Lys Ser Ser Ala
165 170 175
Ala Ser Phe Gly Ser Gly Ser Tyr Ser Ser Gly Ser His Tyr Gly Ser
180 185 190
Thr Gly Gly Ser Arg Glu Val Gly Ser Phe Lys Asp Ile His Thr Gly
195 200 205
Thr Glu Trp Lys Lys Asn Lys Lys Glu Thr Val Ser Asn Tyr Ser Ser
210 215 220
Asn Arg Glu Gly Ser Lys Glu Ile Thr Asn Ser Ala Thr Ser Tyr Lys
225 230 235 240
Ser Lys Lys Ser Glu Arg His Gly Arg Arg Asn Gln Asn Ser Leu Thr
245 250 255
Leu His Ser Lys Leu Ser Ala Asn Ile Ser Thr Thr Ser Glu Ala Pro
260 265 270
Ser Ser Lys Lys Gly Glu Asn Glu Asp Asp Asp Asp Phe Asn Pro Arg
275 280 285
Gly Phe Ser Thr Ser Thr Gly Thr Gly Thr Thr Arg Ser Asn His Leu
290 295 300
Asp Leu Phe Gly Pro Ser Leu Met Asp Asp Leu Val Asp Ser Thr Thr
305 310 315 320
Ser Thr Ser Thr Ala Thr Pro Asn Val Ser Thr Pro Ala Val Pro Glu
325 330 335
Val Asp Leu Phe Ala Asp Ala Ala Phe Gln Ser Ala Asn Ala Pro Leu
340 345 350
Glu Ala Ala Thr Val Ser His Thr Gln Asp Lys Ile Asp Leu Phe Ala
355 360 365
Gly Arg Leu Ser Ser Ala Asp Ser Phe Thr Ser Asp Thr Glu Phe Ser
370 375 380
Val Arg Gly Ser Pro Asn Lys Ser Ser Glu Lys Lys Met Ser Ser Val
385 390 395 400
Val His Pro Ser Thr Ser Ala Phe Asp Pro Phe Lys Gln Ser Phe Ala
405 410 415
Thr Ser Phe Pro Ser Asp Ser Glu Phe Ser Val His Asp Pro Thr Ser
420 425 430
Lys Ser Ser Gln Gly Lys Thr Pro Thr Pro Glu His Ser Ser Thr Ala
435 440 445
Ala Phe Asp Pro Phe Ala Ala Ile Pro Leu Lys Ser Phe Asp Gly Ser
450 455 460
Glu Ser Phe Gly Thr Phe Ser Ser Asn Thr Ala Ser Asn Ile Thr Glu
465 470 475 480
Leu Pro Arg Asp Ser Ser Gly Gly Pro Lys Ser Ser Asp His Gly Pro
485 490 495
Leu Glu Asp Ala Asn Phe Asp Ala Phe Thr Ser His Leu Gly Ser Ser
500 505 510
Thr Thr Ser Ala Thr Glu Ser Met Asn Lys Pro Ile Lys Lys Leu Gly
515 520 525
Gln Asp Ser Met Ser Ala Ser Lys Ser Val Ala Lys Lys Glu Thr Phe
530 535 540
Gln Val Lys Ser Gly Ile Trp Ala Asp Ser Leu Ser Arg Gly Leu Ile
545 550 555 560
Asp Leu Asn Ile Thr Ser Ser Gln Lys Lys Val Asp Leu Ser Asp Val
565 570 575
Gly Ile Val Gly Pro Leu Ser Gly Gly Ser Glu Asp Lys Gly Pro Gly
580 585 590
Ala Thr Met Gly Thr Ala Pro Gly Leu Val Ser Ser Ser Phe Pro Ser
595 600 605
Lys Thr Glu Thr Ser Ser Gly Ser Gly His Phe Gln His Gln Gln Phe
610 615 620
Gly Ser Phe Lys
625
<210>73
<211>1917
<212>DNA
<213>野燕麦(Avena fatua)
<400>73
atggatttca tgaaggtctt cgatcagacc gtgcgagaga tcaagaggga ggtcaacctg 60
aaggtgctca aggttcccga gctcgaacag aaggtgcttg atgcaacaag tgatgaacct 120
tggggtccac atggaagtgc tctttcagac gtagcgcagg ccaccaagaa atactctgag 180
tgtcagatgg taatgggtgt cctttgggct aggcttgctg agcgagactc gaactggcgc 240
catgtgtata aggcgctgac aattatcgag tacttgatag ccaacggttc tgagcgggcg 300
gttgataaca tccttgatca tttttcaaag atctcggttc tttcgagttt tgagtttgtg 360
gaaccgaatg gaaaagatgc tggaataaat gtgaggaaga aagtcgaaac tctagtgggc 420
atcataaatg acaaggatag aataaaggct gtgagagata aagcagccag taaccgtgac 480
aagtacgttg ggttatcctc aaccggatca tcatatagat caagctcagc cactgtaggg 540
agcaattaca gttcaggtga acgttatggg agttttggtg gcacaaggga gggtgattcg 600
ttcagcaaca gttacaagga caaagaatct gctaaaacct ctgcaggtag taatggcagc 660
aagaaatctg gcagcaagac aagaaaagat gcgaagcatg atagaagctc ctcgaagcca 720
ccatctactg caaaaagcaa tgaagatgac ttcgatgact ttgatccccg tggatcttct 780
tcaaatgatg cagctaacaa tgcgaagact agcgaggtcg atctttttgg cccaaacttg 840
atggatgatt tcatggatga gcctgcagcc actccagcaa caaaaggtgt tgtagaacct 900
caggttgatc tatttggtga tgcagatttc caatcagcaa ccccaagtgc agaaactgct 960
gcgcatcagg atgtccagga caatgttgac ctttttgcgg gaaatgcaac ctttgcttca 1020
gcatttccat cacagacggg gtttattcca ccaccaagtt ccgggacatc ttcttctgcc 1080
aatagttttg tgtccaagaa gacagtacct gaaccatttg atcctttcgg tgatattcct 1140
ttaagcagtt ttggaggatc tgacccattt ggtgacttca gttccaatgt tggatcatct 1200
actgcaccgc cagctgtaca caattccact ggaaatatca gcacgccgag tcagaactct 1260
caggcagcat cagactttgg cgcctttgaa tctaacaccg gaggcgcagc taaagatccc 1320
tttgattttt cttccggtgg caattttggc aaaacaaacg taacaccttt ggcagctccc 1380
aaaaccgaca cgtctgattt cggtgcattc gtagcaaaca ctgcggaacg tgctaaggac 1440
ccctttgatc tttcttctag catcaacaat ggaaggacag gccaaacacc tctggcagct 1500
cccaagtcta acaccaagaa agaaaatctc caggtcaaat ctagcatatg ggccgactct 1560
ttgagccgtg gattgattga tctgaacata accggaccaa agaaggtgaa cttggccgac 1620
gttgggatcg tgggcggcct cggtgatggg tcagatgaca aggctcatca gccctcctgg 1680
aacatgggcg gcggggggat ctggtctagg gatgtctgga atccctccat ctacacaagg 1740
cggcggcatc gagagcttgg cgaactacaa caagtaccag tttggcttca aatgagcgtt 1800
caggcgagag cgtttgtagt attctggtgc tcttgttgcc gggctgttaa atgcatattg 1860
atttcttttc ttttgttata tacatctgtt ctggtgattt cattatgtta cctgtaa 1917
<210>74
<211>638
<212>PRT
<213>野燕麦
<400>74
Met Asp Phe Met Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Leu Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly Ser Ala Leu
35 40 45
Ser Asp Val Ala Gln Ala Thr Lys Lys Tyr Ser Glu Cys Gln Met Val
50 55 60
Met Gly Val Leu Trp Ala Arg Leu Ala Glu Arg Asp Ser Asn Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Thr Ile Ile Glu Tyr Leu Ile Ala Asn Gly
85 90 95
Ser Glu Arg Ala Val Asp Asn Ile Leu Asp His Phe Ser Lys Ile Ser
100 105 110
Val Leu Ser Ser Phe Glu Phe Val Glu Pro Asn Gly Lys Asp Ala Gly
115 120 125
Ile Asn Val Arg Lys Lys Val Glu Thr Leu Val Gly Ile Ile Asn Asp
130 135 140
Lys Asp Arg Ile Lys Ala Val Arg Asp Lys Ala Ala Ser Asn Arg Asp
145 150 155 160
Lys Tyr Val Gly Leu Ser Ser Thr Gly Ser Ser Tyr Arg Ser Ser Ser
165 170 175
Ala Thr Val Gly Ser Asn Tyr Ser Ser Gly Glu Arg Tyr Gly Ser Phe
180 185 190
Gly Gly Thr Arg Glu Gly Asp Ser Phe Ser Asn Ser Tyr Lys Asp Lys
195 200 205
Glu Ser Ala Lys Thr Ser Ala Gly Ser Asn Gly Ser Lys Lys Ser Gly
210 215 220
Ser Lys Thr Arg Lys Asp Ala Lys His Asp Arg Ser Ser Ser Lys Pro
225 230 235 240
Pro Ser Thr Ala Lys Ser Asn Glu Asp Asp Phe Asp Asp Phe Asp Pro
245 250 255
Arg Gly Ser Ser Ser Asn Asp Ala Ala Asn Asn Ala Lys Thr Ser Glu
260 265 270
Val Asp Leu Phe Gly Pro Asn Leu Met Asp Asp Phe Met Asp Glu Pro
275 280 285
Ala Ala Thr Pro Ala Thr Lys Gly Val Val Glu Pro Gln Val Asp Leu
290 295 300
Phe Gly Asp Ala Asp Phe Gln Ser Ala Thr Pro Ser Ala Glu Thr Ala
305 310 315 320
Ala His Gln Asp Val Gln Asp Asn Val Asp Leu Phe Ala Gly Asn Ala
325 330 335
Thr Phe Ala Ser Ala Phe Pro Ser Gln Thr Gly Phe Ile Pro Pro Pro
340 345 350
Ser Ser Gly Thr Ser Ser Ser Ala Asn Ser Phe Val Ser Lys Lys Thr
355 360 365
Val Pro Glu Pro Phe Asp Pro Phe Gly Asp Ile Pro Leu Ser Ser Phe
370 375 380
Gly Gly Ser Asp Pro Phe Gly Asp Phe Ser Ser Asn Val Gly Ser Ser
385 390 395 400
Thr Ala Pro Pro Ala Val His Asn Ser Thr Gly Asn Ile Ser Thr Pro
405 410 415
Ser Gln Asn Ser Gln Ala Ala Ser Asp Phe Gly Ala Phe Glu Ser Asn
420 425 430
Thr Gly Gly Ala Ala Lys Asp Pro Phe Asp Phe Ser Ser Gly Gly Asn
435 440 445
Phe Gly Lys Thr Asn Val Thr Pro Leu Ala Ala Pro Lys Thr Asp Thr
450 455 460
Ser Asp Phe Gly Ala Phe Val Ala Asn Thr Ala Glu Arg Ala Lys Asp
465 470 475 480
Pro Phe Asp Leu Ser Ser Ser Ile Asn Asn Gly Arg Thr Gly Gln Thr
485 490 495
Pro Leu Ala Ala Pro Lys Ser Asn Thr Lys Lys Glu Asn Leu Gln Val
500 505 510
Lys Ser Ser Ile Trp Ala Asp Ser Leu Ser Arg Gly Leu Ile Asp Leu
515 520 525
Asn Ile Thr Gly Pro Lys Lys Val Asn Leu Ala Asp Val Gly Ile Val
530 535 540
Gly Gly Leu Gly Asp Gly Ser Asp Asp Lys Ala His Gln Pro Ser Trp
545 550 555 560
Asn Met Gly Gly Gly Gly Ile Trp Ser Arg Asp Val Trp Asn Pro Ser
565 570 575
Ile Tyr Thr Arg Arg Arg His Arg Glu Leu Gly Glu Leu Gln Gln Val
580 585 590
Pro Val Trp Leu Gln Met Ser Val Gln Ala Arg Ala Phe Val Val Phe
595 600 605
Trp Cys Ser Cys Cys Arg Ala Val Lys Cys Ile Leu Ile Ser Phe Leu
610 615 620
Leu Leu Tyr Thr Ser Val Leu Val Ile Ser Leu Cys Tyr Leu
625 630 635
<210>75
<211>2910
<212>DNA
<213>蒺藜苜蓿
<400>75
atgaagaagg tttttggtca aactgttagg gaccttaaaa gagaagtgaa caagaaagtg 60
ctcaaagttc ctggcattga acagaaggtt cttgatgcta ctagcaatga accatggggt 120
cctcatggaa cactgcttgc agacattgct caggcaacta gaaaccctca tgaataccag 180
atgatcatgt cagtagtctg gaaacgaatt aacgacactg gcaagaattg gcggcatgtc 240
tacaaggctt tgacagtact tgagtacttg gtggcccatg gttctgagag agtcatagat 300
gagatcaaag aacattctta tcaaatatcg actttgtccg actttcaata tattgattcc 360
agtggaagag atcaaggaaa caatgtcagg aaaaaatctc agaatctcgt tgtccttgtg 420
aatgataaag aaagaatcgt agaagttaga cagaaggctg ctgttaatag ggaaaagttt 480
cgcaataaca caccaggagg aatgtataga cctggttcac actcaagcat tggaagctat 540
ggtgatcgat atgaagaaga tcgttatgcg aacagggagg aagatagaaa tggctatggt 600
tatggaagag aaagagaaat gggctctaga gatgatgatc ggtacaatcg cgatggggat 660
cgttatggta gagattatga ggaacgttat ggtagggacg gttacagaga tgatgatagg 720
ggaagaagtc gaagtgttga ctataattat gatgatacta ggagcaggaa ttctgataga 780
gatcgtgatt tcgatgacga tggccaacac tcgtctcgag gtagcaatgc taaagttgaa 840
gaccaatcac tggaagcaag acttcagcgg aaactttctg agcaaaattc gggtgctcct 900
cctagttatg aagaggctgt tggtgaagct cagagccctg tgcctgaaag ggatgttgaa 960
acttcggcag aatctgctcc aagaggctcg tcccctcatg caagtgataa tcctagccca 1020
gcctctgctc ctactggatc ttctcctgta agcaataatc caactgaagt aactgctgct 1080
gccagtacat ctgttgctgc tagtactgct gcttctaccc aggaaactga gcccactgat 1140
gatttctttg acccacgtgg tcctacatca gctgctccaa ccacctctaa ttttggcgaa 1200
atagatttgc tcggttcttt atcagactca ttctcttcaa acgctctgcc tcttgtgccc 1260
gctacatcag gaatttcaac ccctgaagca aacactggtt caacagcttc ttttgcagca 1320
ccatcctctg ggtccaataa tttcaatcag tcttttgaag atccgtttgg tgattcacca 1380
ttcaaggctg atacttctgt tgaaactgct ccatctcaac accatgcacc tcagactact 1440
gaaccatccc aatcagatgg ttttaatgct gacatgtcta actttgggtt tggggactca 1500
ttttctattg taccatactc tgcatctgct cccagtgaca ctcaaccttt ctctgctaac 1560
tcccagtttt tgtcccaaga ttcggaaact gatattctag ctgacattct tcctcctgca 1620
ccattacctg agattacatc acagcagaac agttcagctc cttcttttgg ccaaccttca 1680
ccttcctttt caacttcatc tggatcattt tccgaaccaa ctggtcaact tactcttcat 1740
caaggtttct cagctgcaac caatcaacct gcacaaaccc ttccaactgg tcaattttcg 1800
cagccaggct tttcagcttc taattcatct ttttcagcct ctaccagtcc atatgcacag 1860
caacctttte cttctcattc tggtcaacct ggcatgcctg ggttttcgtc atctactgga 1920
cattctatgc agccaccttt tgcttctcaa ggcggtcaat ctactgcaca aacaagcggt 1980
catacctacg gtggattgta ctccctggat acatctctta ctccaggagc tccaaacatg 2040
tattctcaat cacaaaatgg atataacgga tctatgaaca gtgggaacta cttgccacag 2100
ggatcctcaa caggctttcc ttcacaaatg actccccaag ctccaacagc acaaccggca 2160
caaatcacaa actttcccca tcatggagga tctactgctt ctccctctcc aactgatcaa 2220
gcatcacagt tcaacaacca gagcttcttt ggacaacaag ggaatgcagc tccctttagc 2280
tcatcatata cccctcaagt gcctgctccc aatgcttcgc cgtatgctgt ttcagcggca 2340
ccaaattctt tagtttctca accatccaaa gataagtttg aaacaaaatc aacagtttgg 2400
gcagacacat taagcagggg actggttaat ttaaacatat ctggacctaa aactaatcca 2460
ttagcagata ttggcattga cttcgaatcc attaatagga aggaaaagag aatggagaag 2520
cccactaaca cacctgtaac atcaactgta aatatgggta aagctatggg atcaggttca 2580
ggcataggtc gtgctggcgc tggtgctctt agacctaatc caaattcgat gatgggttct 2640
ggtatgggca tgggtatggg aatgggcaat gctcccggtg gtatgggtat gggcaatgct 2700
cccggtggta tgggtatggg aggttacgga ggaggcatga atccatccat gggtatgggt 2760
atgggtatgg ggggaatggg catggggcaa ggataccaga tgcaacctcc taatggaatg 2820
cctcctggtt ccaacatgcc aggtaactat aataataaca tgatgcgtcc aggtggttat 2880
gctcaacagc catatggtgg ataccgatga 2910
<210>76
<211>969
<212>PRT
<213>蒺藜苜蓿
<400>76
Met Lys Lys Val Phe Gly Gln Thr Val Arg Asp Leu Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Ile Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Thr Leu Leu Ala Asp
35 40 45
Ile Ala Gln Ala Thr Arg Asn Pro His Glu Tyr Gln Met Ile Met Ser
50 55 60
Val Val Trp Lys Arg Ile Asn Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Leu Val Ala His Gly Ser Glu
85 90 95
Arg Val Ile Asp Glu Ile Lys Glu His Ser Tyr Gln Ile Ser Thr Leu
100 105 110
Ser Asp Phe Gln Tyr Ile Asp Ser Ser Gly Arg Asp Gln Gly Asn Asn
115 120 125
Val Arg Lys Lys Ser Gln Asn Leu Val Val Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Val Glu Val Arg Gln Lys Ala Ala Val Asn Arg Glu Lys Phe
145 150 155 160
Arg Asn Asn Thr Pro Gly Gly Met Tyr Arg Pro Gly Ser His Ser Ser
165 170 175
Ile Gly Ser Tyr Gly Asp Arg Tyr Glu Glu Asp Arg Tyr Ala Asn Arg
180 185 190
Glu Glu Asp Arg Asn Gly Tyr Gly Tyr Gly Arg Glu Arg Glu Met Gly
195 200 205
Ser Arg Asp Asp Asp Arg Tyr Asn Arg Asp Gly Asp Arg Tyr Gly Arg
210 215 220
Asp Tyr Glu Glu Arg Tyr Gly Arg Asp Gly Tyr Arg Asp Asp Asp Arg
225 230 235 240
Gly Arg Ser Arg Ser Val Asp Tyr Asn Tyr Asp Asp Thr Arg Ser Arg
245 250 255
Asn Ser Asp Arg Asp Arg Asp Phe Asp Asp Asp Gly Gln His Ser Ser
260 265 270
Arg Gly Ser Asn Ala Lys Val Glu Asp Gln Ser Leu Glu Ala Arg Leu
275 280 285
Gln Arg Lys Leu Ser Glu Gln Asn Ser Gly Ala Pro Pro Ser Tyr Glu
290 295 300
Glu Ala Val Gly Glu Ala Gln Ser Pro Val Pro Glu Arg Asp Val Glu
305 310 315 320
Thr Ser Ala Glu Ser Ala Pro Arg Gly Ser Ser Pro His Ala Ser Asp
325 330 335
Asn Pro Ser Pro Ala Ser Ala Pro Thr Gly Ser Ser Pro Val Ser Asn
340 345 350
Asn Pro Thr Glu Val Thr Ala Ala Ala Ser Thr Ser Val Ala Ala Ser
355 360 365
Thr Ala Ala Ser Thr Gln Glu Thr Glu Pro Thr Asp Asp Phe Phe Asp
370 375 380
Pro Arg Gly Pro Thr Ser Ala Ala Pro Thr Thr Ser Asn Phe Gly Glu
385 390 395 400
Ile Asp Leu Leu Gly Ser Leu Ser Asp Ser Phe Ser Ser Asn Ala Leu
405 410 415
Pro Leu Val Pro Ala Thr Ser Gly Ile Ser Thr Pro Glu Ala Asn Thr
420 425 430
Gly Ser Thr Ala Ser Phe Ala Ala Pro Ser Ser Gly Ser Asn Asn Phe
435 440 445
Asn Gln Ser Phe Glu Asp Pro Phe Gly Asp Ser Pro Phe Lys Ala Asp
450 455 460
Thr Ser Val Glu Thr Ala Pro Ser Gln His His Ala Pro Gln Thr Thr
465 470 475 480
Glu Pro Ser Gln Ser Asp Gly Phe Asn Ala Asp Met Ser Asn Phe Gly
485 490 495
Phe Gly Asp Ser Phe Ser Ile Val Pro Tyr Ser Ala Ser Ala Pro Ser
500 505 510
Asp Thr Gln Pro Phe Ser Ala Asn Ser Gln Phe Leu Ser Gln Asp Ser
515 520 525
Glu Thr Asp Ile Leu Ala Asp Ile Leu Pro Pro Ala Pro Leu Pro Glu
530 535 540
Ile Thr Ser Gln Gln Asn Ser Ser Ala Pro Ser Phe Gly Gln Pro Ser
545 550 555 560
Pro Ser Phe Ser Thr Ser Ser Gly Ser Phe Ser Glu Pro Thr Gly Gln
565 570 575
Leu Thr Leu His Gln Gly Phe Ser Ala Ala Thr Asn Gln Pro Ala Gln
580 585 590
Thr Leu Pro Thr Gly Gln Phe Ser Gln Pro Gly Phe Ser Ala Ser Asn
595 600 605
Ser Ser Phe Ser Ala Ser Thr Ser Pro Tyr Ala Gln Gln Pro Phe Pro
610 615 620
Ser His Ser Gly Gln Pro Gly Met Pro Gly Phe Ser Ser Ser Thr Gly
625 630 635 640
His Ser Met Gln Pro Pro Phe Ala Ser Gln Gly Gly Gln Ser Thr Ala
645 650 655
Gln Thr Ser Gly His Thr Tyr Gly Gly Leu Tyr Ser Leu Asp Thr Ser
660 665 670
Leu Thr Pro Gly Ala Pro Asn Met Tyr Ser Gln Ser Gln Asn Gly Tyr
675 680 685
Asn Gly Ser Met Asn Ser Gly Asn Tyr Leu Pro Gln Gly Ser Ser Thr
690 695 700
Gly Phe Pro Ser Gln Met Thr Pro Gln Ala Pro Thr Ala Gln Pro Ala
705 710 715 720
Gln Ile Thr Asn Phe Pro His His Gly Gly Ser Thr Ala Ser Pro Ser
725 730 735
Pro Thr Asp Gln Ala Ser Gln Phe Asn Asn Gln Ser Phe Phe Gly Gln
740 745 750
Gln Gly Asn Ala Ala Pro Phe Ser Ser Ser Tyr Thr Pro Gln Val Pro
755 760 765
Ala Pro Asn Ala Ser Pro Tyr Ala Val Ser Ala Ala Pro Asn Ser Leu
770 775 780
Val Ser Gln Pro Ser Lys Asp Lys Phe Glu Thr Lys Ser Thr Val Trp
785 790 795 800
Ala Asp Thr Leu Ser Arg Gly Leu Val Asn Leu Asn Ile Ser Gly Pro
805 810 815
Lys Thr Asn Pro Leu Ala Asp Ile Gly Ile Asp Phe Glu Ser Ile Asn
820 825 830
Arg Lys Glu Lys Arg Met Glu Lys Pro Thr Asn Thr Pro Val Thr Ser
835 840 845
Thr Val Asn Met Gly Lys Ala Met Gly Ser Gly Ser Gly Ile Gly Arg
850 855 860
Ala Gly Ala Gly Ala Leu Arg Pro Asn Pro Asn Ser Met Met Gly Ser
865 870 875 880
Gly Met Gly Met Gly Met Gly Met Gly Asn Ala Pro Gly Gly Met Gly
885 890 895
Met Gly Asn Ala Pro Gly Gly Met Gly Met Gly Gly Tyr Gly Gly Gly
900 905 910
Met Asn Pro Ser Met Gly Met Gly Met Gly Met Gly Gly Met Gly Met
915 920 925
Gly Gln Gly Tyr Gln Met Gln Pro Pro Asn Gly Met Pro Pro Gly Ser
930 935 940
Asn Met Pro Gly Asn Tyr Asn Asn Asn Met Met Arg Pro Gly Gly Tyr
945 950 955 960
Ala Gln Gln Pro Tyr Gly Gly Tyr Arg
965
<210>77
<211>2688
<212>DNA
<213>拟南芥
<400>77
atgaagaaag tcttcggaca aactgttaga gaccttaaga gagaggtcaa caagaaagtg 60
ctcaaagttc ctggagtaga acagaaggtt ctagatgcta ctagtaatga gccatggggt 120
ccacatggat cacttcttgc tgatcttgcg caagcttcca gaaattatca tgaatatcag 180
ctgatcatgg tggtcatatg gaaacgcctt agcgacaccg gaaaaaactg gcggcatgtc 240
tataaggctt tgacagtttt ggagtacatg gtaggccatg gatcagaacg tgttatagac 300
gagattagag aacgtgcata tcaaatttcg acattgtccg attttcagta tattgattcc 360
ggtggtagag atcaaggaag caatgttagg aagaaatcac agagcctggt ggctttggtg 420
aatgacaagg aaagaatagc tgaagtcaga cagaaggctg ctgctaatag agataagtat 480
cgcagctcag caccaggtgg gatgtataag ccttcaggcg gatatgggga taaatatgat 540
tacggaagcc gggatgaaga gcgaagtagt tatggaagag aaagagaata tggttacaga 600
gatgatgata gaaatagtcg tgatggagat cgtcattcca gggactctga agaccggtat 660
gggagagatg gtaataggga tgatgattac aggggaagga gcagaagtgt tgataactat 720
gggtcacgag gtaggagttc ggaaagagag cgggaggatg atggccattc ttcatcacgg 780
ggcagtggtg ctcgagctga tgataattct caggatggga gaggggggct ccagaggaag 840
ttttctgaac aaaatattgg cgctccaccc aattatgaag aagctgtcag tgattcacgc 900
agccctgtat atagtgaaag ggatggtggg gagaccccac aagttaccgc tccaggagct 960
gcttctcctc ctcccccaca agttgctgct ccagaggctg cttctcctcc aactggaacc 1020
aacacagcca atacaactgc tacttttgtt aatgaatcac cttctcagaa agttgagact 1080
ttcgatgaat ttgatccacg cagtgcgttt tcagctggcc ctccagcata tgcatctaca 1140
gatggtgtta cagctcctcc aactgtgact tctatgtctg cccctaccac ctcaaacagt 1200
gttgagatgg acttgcttgg ttcccttgca gacgtatttt catcaaacgc attggccatt 1260
gtaccagctg attctatcta tgttgaaacc aatggacaag caaacgctgg tccagctcca 1320
tcgttttcta catctcagcc atcaactcag tcatttgatg acccatttgg tgactctcct 1380
ttcaaggcct tcacttctac tgacaccgac tcaaccccac agcagaattt tggagcttct 1440
ttccagccac cacctccagc cttcacttca gaggtctcac atcctgatac tgctcataac 1500
tttggctttg gggactcatt ttccgccgtt gccaatcctg atcctgcttc tcagaatgtg 1560
caacctccat cgaactcacc aggttttcct caagagcaat ttgctacatc tcagagtggt 1620
attgatatcc tcgctggcat tctcccacca tctggacctc cagtacaatc gggtccctca 1680
ataccaacat ctcaatttcc tcccagtgga aacaacatgt acgagggatt tcattctcag 1740
ccgccggtat ctacagctcc aaacctgcct ggacaaactc catttggaca agctgttcaa 1800
ccatataaca tggttcctca ttctcaaaat atgactggag ccatgccatt taatagtgga 1860
ggcttcatgc accagccggg ctcacaaact ccttactcta ccccaagtgg accagctggt 1920
cagttcatgg cacaccaagg tcatggaatg ccgccatccc atggtccaca acgaactcaa 1980
tccggacctg ttactctgca agggaacaac aatgtcatgg gagacatgtt ctcgcaagct 2040
acgcccaact ccttgacgtc atcatcatct catccagatc tcacaccttt aacaggagca 2100
attgagattg ttcctccgcc tcagaaaaag tttgagccaa aatcatcagt ctgggcagac 2160
acgttgagca gggggcttgt taactttaac atatctggat ctaaaacaaa tccattggca 2220
gacataggag ttgacttcga ggcgatcaac aggagagaga aacggctgga gaaacaaaca 2280
aacacaccag caacatcgac tatcaatatg ggtaaagcca tgggatcagg cactggctta 2340
gggcgttctg gtgcaactgc tatgagacct ccacctaatc caatgacagg ctctggcatg 2400
cccatgggcg gaggaatggg tgttggtagc tacggaggta tgaaccagaa ccaacccatg 2460
ggtatgggta tgggggccgg aatgaaccaa aaccaaccga tgggtatggg aatgggaccc 2520
ggcatgaaca tgaacatgaa catgggagga tatggccaag gctatccgat gcaaccacaa 2580
aacccaggga tggtacctag cccaaacatg cctggcaata actacaatcc gatgatgggt 2640
caaggtggtt acaaccctca acaatcctat ggtggtggat accggtaa 2688
<210>78
<211>895
<212>PRT
<213>拟南芥
<400>78
Met Lys Lys Val Phe Gly Gln Thr Val Arg Asp Leu Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Val Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser Leu Leu Ala Asp
35 40 45
Leu Ala Gln Ala Ser Arg Asn Tyr His Glu Tyr Gln Leu Ile Met Val
50 55 60
Val Ile Trp Lys Arg Leu Ser Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Met Val Gly His Gly Ser Glu
85 90 95
Arg Val Ile Asp Glu Ile Arg Glu Arg Ala Tyr Gln Ile Ser Thr Leu
100 105 110
Ser Asp Phe Gln Tyr Ile Asp Ser Gly Gly Arg Asp Gln Gly Ser Asn
115 120 125
Val Arg Lys Lys Ser Gln Ser Leu Val Ala Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Ala Glu Val Arg Gln Lys Ala Ala Ala Asn Arg Asp Lys Tyr
145 150 155 160
Arg Ser Ser Ala Pro Gly Gly Met Tyr Lys Pro Ser Gly Gly Tyr Gly
165 170 175
Asp Lys Tyr Asp Tyr Gly Ser Arg Asp Glu Glu Arg Ser Ser Tyr Gly
180 185 190
Arg Glu Arg Glu Tyr Gly Tyr Arg Asp Asp Asp Arg Asn Ser Arg Asp
195 200 205
Gly Asp Arg His Ser Arg Asp Ser Glu Asp Arg Tyr Gly Arg Asp Gly
210 215 220
Asn Arg Asp Asp Asp Tyr Arg Gly Arg Ser Arg Ser Val Asp Asn Tyr
225 230 235 240
Gly Ser Arg Gly Arg Ser Ser Glu Arg Glu Arg Glu Asp Asp Gly His
245 250 255
Ser Ser Ser Arg Gly Ser Gly Ala Arg Ala Asp Asp Asn Ser Gln Asp
260 265 270
Gly Arg Gly Gly Leu Gln Arg Lys Phe Ser Glu Gln Asn Ile Gly Ala
275 280 285
Pro Pro Asn Tyr Glu Glu Ala Val Ser Asp Ser Arg Ser Pro Val Tyr
290 295 300
Ser Glu Arg Asp Gly Gly Glu Thr Pro Gln Val Thr Ala Pro Gly Ala
305 310 315 320
Ala Ser Pro Pro Pro Pro Gln Val Ala Ala Pro Glu Ala Ala Ser Pro
325 330 335
Pro Thr Gly Thr Asn Thr Ala Asn Thr Thr Ala Thr Phe Val Asn Glu
340 345 350
Ser Pro Ser Gln Lys Val Glu Thr Phe Asp Glu Phe Asp Pro Arg Ser
355 360 365
Ala Phe Ser Ala Gly Pro Pro Ala Tyr Ala Ser Thr Asp Gly Val Thr
370 375 380
Ala Pro Pro Thr Val Thr Ser Met Ser Ala Pro Thr Thr Ser Asn Ser
385 390 395 400
Val Glu Met Asp Leu Leu Gly Ser Leu Ala Asp Val Phe Ser Ser Asn
405 410 415
Ala Leu Ala Ile Val Pro Ala Asp Ser Ile Tyr Val Glu Thr Asn Gly
420 425 430
Gln Ala Asn Ala Gly Pro Ala Pro Ser Phe Ser Thr Ser Gln Pro Ser
435 440 445
Thr Gln Ser Phe Asp Asp Pro Phe Gly Asp Ser Pro Phe Lys Ala Phe
450 455 460
Thr Ser Thr Asp Thr Asp Ser Thr Pro Gln Gln Asn Phe Gly Ala Ser
465 470 475 480
Phe Gln Pro Pro Pro Pro Ala Phe Thr Ser Glu Val Ser His Pro Asp
485 490 495
Thr Ala His Asn Phe Gly Phe Gly Asp Ser Phe Ser Ala Val Ala Asn
500 505 510
Pro Asp Pro Ala Ser Gln Asn Val Gln Pro Pro Ser Asn Ser Pro Gly
515 520 525
Phe Pro Gln Glu Gln Phe Ala Thr Ser Gln Ser Gly Ile Asp Ile Leu
530 535 540
Ala Gly Ile Leu Pro Pro Ser Gly Pro Pro Val Gln Ser Gly Pro Ser
545 550 555 560
Ile Pro Thr Ser Gln Phe Pro Pro Ser Gly Asn Asn Met Tyr Glu Gly
565 570 575
Phe His Ser Gln Pro Pro Val Ser Thr Ala Pro Asn Leu Pro Gly Gln
580 585 590
Thr Pro Phe Gly Gln Ala Val Gln Pro Tyr Asn Met Val Pro His Ser
595 600 605
Gln Asn Met Thr Gly Ala Met Pro Phe Asn Ser Gly Gly Phe Met His
610 615 620
Gln Pro Gly Ser Gln Thr Pro Tyr Ser Thr Pro Ser Gly Pro Ala Gly
625 630 635 640
Gln Phe Met Ala His Gln Gly His Gly Met Pro Pro Ser His Gly Pro
645 650 655
Gln Arg Thr Gln Ser Gly Pro Val Thr Leu Gln Gly Asn Asn Asn Val
660 665 670
Met Gly Asp Met Phe Ser Gln Ala Thr Pro Asn Ser Leu Thr Ser Ser
675 680 685
Ser Ser His Pro Asp Leu Thr Pro Leu Thr Gly Ala Ile Glu Ile Val
690 695 700
Pro Pro Pro Gln Lys Lys Phe Glu Pro Lys Ser Ser Val Trp Ala Asp
705 710 715 720
Thr Leu Ser Arg Gly Leu Val Asn Phe Asn Ile Ser Gly Ser Lys Thr
725 730 735
Asn Pro Leu Ala Asp Ile Gly Val Asp Phe Glu Ala Ile Asn Arg Arg
740 745 750
Glu Lys Arg Leu Glu Lys Gln Thr Asn Thr Pro Ala Thr Ser Thr Ile
755 760 765
Asn Met Gly Lys Ala Met Gly Ser Gly Thr Gly Leu Gly Arg Ser Gly
770 775 780
Ala Thr Ala Met Arg Pro Pro Pro Asn Pro Met Thr Gly Ser Gly Met
785 790 795 800
Pro Met Gly Gly Gly Met Gly Val Gly Ser Tyr Gly Gly Met Asn Gln
805 810 815
Asn Gln Pro Met Gly Met Gly Met Gly Ala Gly Met Asn Gln Asn Gln
820 825 830
Pro Met Gly Met Gly Met Gly Pro Gly Met Asn Met Asn Met Asn Met
835 840 845
Gly Gly Tyr Gly Gln Gly Tyr Pro Met Gln Pro Gln Asn Pro Gly Met
850 855 860
Val Pro Ser Pro Asn Met Pro Gly Asn Asn Tyr Asn Pro Met Met Gly
865 870 875 880
Gln Gly Gly Tyr Asn Pro Gln Gln Ser Tyr Gly Gly Gly Tyr Arg
885 890 895
<210>79
<211>2688
<212>DNA
<213>拟南芥
<400>79
atgaagaaag tcttcggaca aactgttaga gaccttaaga gagaggtcaa caagaaagtg 60
ctcaaagttc ctggagtaga acagaaggtt ctagatgcta ctagtaatga gccatggggt 120
ccacatggat cacttcttgc tgatcttgcg caagcttcca gaaattatca tgaatatcag 180
ctgatcatgg tggtcatatg gaaacgcctt agcgacaccg gaaaaaactg gcggcatgtc 240
tataaggctt tgacagtttt ggagtacatg gtaggccatg gatcagaacg tgttatagac 300
gagattagag aacgtgcata tcaaatttcg acattgtccg attttcagta tattgattcc 360
ggtggtagag atcaaggaag caatgttagg aagaaatcac agagcctggt ggctttggtg 420
aatgacaagg aaagaatagc tgaagtcaga cagaaggctg ctgctaatag agataagtat 480
cgcagctcag caccaggtgg gatgtataag ccttcaggcg gatatgggga taaatatgat 540
tacggaagcc gggatgaaga gcgaagtagt tatggaagag aaagagaata tggttacaga 600
gatgatgata gaaatagtcg tgatggagat cgtcattcca gggactctga agaccggtat 660
gggagagatg gtaataggga tgatgattac aggggaagga gcagaagtgt tgataactat 720
gggtcacgag gtaggagttc ggaaagagag cgggaggatg atggccattc ttcatcacgg 780
ggcagtggtg ctcgagctga tgataattct caggatggga gaggggggct ccagaggaag 840
ttttctgaac aaaatattgg cgctccaccc agttatgaag aagctgtcag tgattcacgc 900
agccctgtat atagtgaaag ggatggtggg gagaccccac aagttaccgc tccaggagct 960
gcttctcctc ctcccccaca agttgctgct ccagaggctg cttctcctcc aactggaacc 1020
aacacagcca atacaactgc tacttttgtt aatgaatcac cttctcagaa agttgagact 1080
ttcgatgaat ttgatccacg cagtgcgttt tcagctggcc ctccagcata tgcatctaca 1140
gatggtgtta cagctcctcc aactgtgact tctatgtctg cccctaccac ctcaaacagt 1200
gttgagatgg acttgcttgg ttcccttgca gacgtatttt catcaaacgc attggccatt 1260
gtaccagctg attctatcta tgttgaaacc aatggacaag caaacgctgg tccagctcca 1320
tcgttttcta catctcagcc atcaactcag tcatttgatg acccatttgg tgactctcct 1380
ttcaaggcct tcacttctac tgacaccgac tcaaccccac agcagaattt tggagcttct 1440
ttccagccac cacctccagc cttcacttca gaggtctcac atcctgatac tgctcataac 1500
tttggctttg gggactcatt ttccgccgtt gccaatcctg atcctgcttc tcagaatgtg 1560
caacctccat cgaactcacc aggttttcct caagagcaat ttgctacatc tcagagtggt 1620
attgatatcc tcgctggcat tctcccacca tctggacctc cagtacaatc gggtccctca 1680
ataccaacat ctcaatttcc tcccagtgga aacaacatgt acgagggatt tcattctcag 1740
ccgccggtat ctacagctcc aaacctgcct ggacaaactc catttggaca agctgttcaa 1800
ccatataaca tggttcctca ttctcaaaat atgactggag ccatgccatt taatagtgga 1860
ggcttcatgc accagccggg ctcacaaact ccttactcta ccccaagtgg accagctggt 1920
cagttcatgg cacaccaagg tcatggaatg ccgccatccc atggtccaca acgaactcaa 1980
tccggacctg ttactctgca agggaacaac aatgtcatgg gagacatgtt ctcgcaagct 2040
acgcccaact ccttgacgtc atcatcatct catccagatc tcacaccttt aacaggagca 2100
attgagattg ttcctccgcc tcagaaaaag tttgagccaa aatcatcagt ctgggcagac 2160
acgttgagca gggggcttgt taactttaac atatctggat ctaaaacaaa tccattggca 2220
gacataggag ttgacttcga ggcgatcaac aggagagaga aacggctgga gaaacaaaca 2280
aacacaccag caacatcgac tatcaatatg ggtaaagcca tgggatcagg cactggctta 2340
gggcgttctg gtgcaactgc tatgagacct ccacctaatc caatgacagg ctctggcatg 2400
cccatgggcg gaggaatggg tgttggtagc tacggaggta tgaaccagaa ccaacccatg 2460
ggtatgggta tgggggccgg aatgaaccaa aaccaaccga tgggtatggg aatgggaccc 2520
ggcatgaaca tgaacatgaa catgggagga tatggccaag gctatccgat gcaaccacaa 2580
aacccaggga tggtacctag cccaaacatg cctggcaata actacaatcc gatgatgggt 2640
caaggtggtt acaaccctca acaatcctat ggtggtggat accggtaa 2688
<210>80
<211>895
<212>PRT
<213>拟南芥
<400>80
Met Lys Lys Val Phe Gly Gln Thr Val Arg Asp Leu Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Val Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser Leu Leu Ala Asp
35 40 45
Leu Ala Gln Ala Ser Arg Asn Tyr His Glu Tyr Gln Leu Ile Met Val
50 55 60
Val Ile Trp Lys Arg Leu Ser Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Met Val Gly His Gly Ser Glu
85 90 95
Arg Val Ile Asp Glu Ile Arg Glu Arg Ala Tyr Gln Ile Ser Thr Leu
100 105 110
Ser Asp Phe Gln Tyr Ile Asp Ser Gly Gly Arg Asp Gln Gly Ser Asn
115 120 125
Val Arg Lys Lys Ser Gln Ser Leu Val Ala Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Ala Glu Val Arg Gln Lys Ala Ala Ala Asn Arg Asp Lys Tyr
145 150 155 160
Arg Ser Ser Ala Pro Gly Gly Met Tyr Lys Pro Ser Gly Gly Tyr Gly
165 170 175
Asp Lys Tyr Asp Tyr Gly Ser Arg Asp Glu Glu Arg Ser Ser Tyr Gly
180 185 190
Arg Glu Arg Glu Tyr Gly Tyr Arg Asp Asp Asp Arg Asn Ser Arg Asp
195 200 205
Gly Asp Arg His Ser Arg Asp Ser Glu Asp Arg Tyr Gly Arg Asp Gly
210 215 220
Asn Arg Asp Asp Asp Tyr Arg Gly Arg Ser Arg Ser Val Asp Asn Tyr
225 230 235 240
Gly Ser Arg Gly Arg Ser Ser Glu Arg Glu Arg Glu Asp Asp Gly His
245 250 255
Ser Ser Ser Arg Gly Ser Gly Ala Arg Ala Asp Asp Asn Ser Gln Asp
260 265 270
Gly Arg Gly Gly Leu Gln Arg Lys Phe Ser Glu Gln Asn Ile Gly Ala
275 280 285
Pro Pro Ser Tyr Glu Glu Ala Val Ser Asp Ser Arg Ser Pro Val Tyr
290 295 300
Ser Glu Arg Asp Gly Gly Glu Thr Pro Gln Val Thr Ala Pro Gly Ala
305 310 315 320
Ala Ser Pro Pro Pro Pro Gln Val Ala Ala Pro Glu Ala Ala Ser Pro
325 330 335
Pro Thr Gly Thr Asn Thr Ala Asn Thr Thr Ala Thr Phe Val Asn Glu
340 345 350
Ser Pro Ser Gln Lys Val Glu Thr Phe Asp Glu Phe Asp Pro Arg Ser
355 360 365
Ala Phe Ser Ala Gly Pro Pro Ala Tyr Ala Ser Thr Asp Gly Val Thr
370 375 380
Ala Pro Pro Thr Val Thr Ser Met Ser Ala Pro Thr Thr Ser Asn Ser
385 390 395 400
Val Glu Met Asp Leu Leu Gly Ser Leu Ala Asp Val Phe Ser Ser Asn
405 410 415
Ala Leu Ala Ile Val Pro Ala Asp Ser Ile Tyr Val Glu Thr Asn Gly
420 425 430
Gln Ala Asn Ala Gly Pro Ala Pro Ser Phe Ser Thr Ser Gln Pro Ser
435 440 445
Thr Gln Ser Phe Asp Asp Pro Phe Gly Asp Ser Pro Phe Lys Ala Phe
450 455 460
Thr Ser Thr Asp Thr Asp Ser Thr Pro Gln Gln Asn Phe Gly Ala Ser
465 470 475 480
Phe Gln Pro Pro Pro Pro Ala Phe Thr Ser Glu Val Ser His Pro Asp
485 490 495
Thr Ala His Asn Phe Gly Phe Gly Asp Ser Phe Ser Ala Val Ala Asn
500 505 510
Pro Asp Pro Ala Ser Gln Asn Val Gln Pro Pro Ser Asn Ser Pro Gly
515 520 525
Phe Pro Gln Glu Gln Phe Ala Thr Ser Gln Ser Gly Ile Asp Ile Leu
530 535 540
Ala Gly Ile Leu Pro Pro Ser Gly Pro Pro Val Gln Ser Gly Pro Ser
545 550 555 560
Ile Pro Thr Ser Gln Phe Pro Pro Ser Gly Asn Asn Met Tyr Glu Gly
565 570 575
Phe His Ser Gln Pro Pro Val Ser Thr Ala Pro Asn Leu Pro Gly Gln
580 585 590
Thr Pro Phe Gly Gln Ala Val Gln Pro Tyr Asn Met Val Pro His Ser
595 600 605
Gln Asn Met Thr Gly Ala Met Pro Phe Asn Ser Gly Gly Phe Met His
610 615 620
Gln Pro Gly Ser Gln Thr Pro Tyr Ser Thr Pro Ser Gly Pro Ala Gly
625 630 635 640
Gln Phe Met Ala His Gln Gly His Gly Met Pro Pro Ser His Gly Pro
645 650 655
Gln Arg Thr Gln Ser Gly Pro Val Thr Leu Gln Gly Asn Asn Asn Val
660 665 670
Met Gly Asp Met Phe Ser Gln Ala Thr Pro Asn Ser Leu Thr Ser Ser
675 680 685
Ser Ser His Pro Asp Leu Thr Pro Leu Thr Gly Ala Ile Glu Ile Val
690 695 700
Pro Pro Pro Gln Lys Lys Phe Glu Pro Lys Ser Ser Val Trp Ala Asp
705 710 715 720
Thr Leu Ser Arg Gly Leu Val Asn Phe Asn Ile Ser Gly Ser Lys Thr
725 730 735
Asn Pro Leu Ala Asp Ile Gly Val Asp Phe Glu Ala Ile Asn Arg Arg
740 745 750
Glu Lys Arg Leu Glu Lys Gln Thr Asn Thr Pro Ala Thr Ser Thr Ile
755 760 765
Asn Met Gly Lys Ala Met Gly Ser Gly Thr Gly Leu Gly Arg Ser Gly
770 775 780
Ala Thr Ala Met Arg Pro Pro Pro Asn Pro Met Thr Gly Ser Gly Met
785 790 795 800
Pro Met Gly Gly Gly Met Gly Val Gly Ser Tyr Gly Gly Met Asn Gln
805 810 815
Asn Gln Pro Met Gly Met Gly Met Gly Ala Gly Met Asn Gln Asn Gln
820 825 830
Pro Met Gly Met Gly Met Gly Pro Gly Met Asn Met Asn Met Asn Met
835 840 845
Gly Gly Tyr Gly Gln Gly Tyr Pro Met Gln Pro Gln Asn Pro Gly Met
850 855 860
Val Pro Ser Pro Asn Met Pro Gly Asn Asn Tyr Asn Pro Met Met Gly
865 870 875 880
Gln Gly Gly Tyr Asn Pro Gln Gln Ser Tyr Gly Gly Gly Tyr Arg
885 890 895
<210>81
<211>1941
<212>DNA
<213>拟南芥
<400>81
atgaagaaag tcttcggaca aactgttaga gaccttaaga gagaggtcaa caagaaagtg 60
ctcaaagttc ctggagtaga acagaaggtt ctagatgcta ctagtaatga gccatggggt 120
ccacatggat cacttcttgc tgatcttgcg caagcttcca gaaattatca tgaatatcag 180
ctgatcatgg tggtcatatg gaaacgcctt agcgacaccg gaaaaaactg gcggcatgtc 240
tataaggctt tgacagtttt ggagtacatg gtaggccatg gatcagaacg tgttatagac 300
gagattagag aacgtgcata tcaaatttcg acattgtccg attttcagta tattgattcc 360
ggtggtagag atcaaggaag caatgttagg aagaaatcac agagcctggt ggctttggtg 420
aatgacaagg aaagaatagc tgaagtcaga cagaaggctg ctgctaatag agataagtat 480
cgcagctcag caccaggtgg gatgtataag ccttcaggcg gatatgggga taaatatgat 540
tacggaagcc gggatgaaga gcgaagtagt tatggaagag aaagagaata tggttacaga 600
gatgatgata gaaatagtcg tgatggagat cgtcattcca gggactctga agaccggtat 660
gggagagatg gtaataggga tgatgattac aggggaagga gcagaagtgt tgataactat 720
gggtcacgag gtaggagttc ggaaagagag cgggaggatg atggccattc ttcatcacgg 780
ggcagtggtg ctcgagctga tgataattct caggatggga gaggggggct ccagaggaag 840
ttttctgaac aaaatattgg cgctccaccc agttatgaag aagctgtcag tgattcacgc 900
agccctgtat atagtgaaag ggatggtggg gagaccccac aagttaccgc tccaggagct 960
gcttctcctc ctcccccaca agttgctgct ccagaggctg cttctcctcc aactggaacc 1020
aacacagcca atacaactgc tacttttgtt aatgaatcac cttctcagaa agttgagact 1080
ttcgatgaat ttgatccacg cagtgcgttt tcagctggcc ctccagcata tgcatctaca 1140
gatggtgtta cagctcctcc aactgtgact tctatgtctg cccctaccac ctcaaacagt 1200
gttgagatgg acttgcttgg ttcccttgca gacgtatttt catcaaacgc attggccatt 1260
gtaccagctg attctatcta tgttgaaacc aatggacaag caaacgctgg tccagctcca 1320
tcgttttcta catctcagcc atcaactcag tcatttgatg acccatttgg tgactctcct 1380
ttcaaggcct tcacttctac tgacaccgac tcaaccccac agcagaattt tggagcttct 1440
ttccagccac cacctccagc cttcacttca gaggtctcac atcctgatac tgctcataac 1500
tttggctttg gggactcatt ttccgccgtt gccaatcctg atcctgcttc tcagaatgtg 1560
caacctccat cgaactcacc aggttttcct caagagcaat ttgctacatc tcagagtggt 1620
attgatatcc tcgctggcat tctcccacca tctggacctc cagtacaatc gggtccctca 1680
ataccaacat ctcaatttcc tcccagtgga aacaacatgt acgaggggtt tcattctcag 1740
ccgccggtat ctacagctcc aaacctgcct ggacaaactc catttggaca agctgttcaa 1800
ccatataaca tggttcctca ttctcaaaat atgactggag ccatgccatt taatagtgga 1860
ggcttcatgc accagccggg ctcacaaact ccttactcta ccccaagtgg accagctggt 1920
cagttcatgg cactggctta g 1941
<210>82
<211>646
<212>PRT
<213>拟南芥
<400>82
Met Lys Lys Val Phe Gly Gln Thr Val Arg Asp Leu Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Val Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser Leu Leu Ala Asp
35 40 45
Leu Ala Gln Ala Ser Arg Asn Tyr His Glu Tyr Gln Leu Ile Met Val
50 55 60
Val Ile Trp Lys Arg Leu Ser Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Met Val Gly His Gly Ser Glu
85 90 95
Arg Val Ile Asp Glu Ile Arg Glu Arg Ala Tyr Gln Ile Ser Thr Leu
100 105 110
Ser Asp Phe Gln Tyr Ile Asp Ser Gly Gly Arg Asp Gln Gly Ser Asn
115 120 125
Val Arg Lys Lys Ser Gln Ser Leu Val Ala Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Ala Glu Val Arg Gln Lys Ala Ala Ala Asn Arg Asp Lys Tyr
145 150 155 160
Arg Ser Ser Ala Pro Gly Gly Met Tyr Lys Pro Ser Gly Gly Tyr Gly
165 170 175
Asp Lys Tyr Asp Tyr Gly Ser Arg Asp Glu Glu Arg Ser Ser Tyr Gly
180 185 190
Arg Glu Arg Glu Tyr Gly Tyr Arg Asp Asp Asp Arg Asn Ser Arg Asp
195 200 205
Gly Asp Arg His Ser Arg Asp Ser Glu Asp Arg Tyr Gly Arg Asp Gly
210 215 220
Asn Arg Asp Asp Asp Tyr Arg Gly Arg Ser Arg Ser Val Asp Asn Tyr
225 230 235 240
Gly Ser Arg Gly Arg Ser Ser Glu Arg Glu Arg Glu Asp Asp Gly His
245 250 255
Ser Ser Ser Arg Gly Ser Gly Ala Arg Ala Asp Asp Asn Ser Gln Asp
260 265 270
Gly Arg Gly Gly Leu Gln Arg Lys Phe Ser Glu Gln Asn Ile Gly Ala
275 280 285
Pro Pro Ser Tyr Glu Glu Ala Val Ser Asp Ser Arg Ser Pro Val Tyr
290 295 300
Ser Glu Arg Asp Gly Gly Glu Thr Pro Gln Val Thr Ala Pro Gly Ala
305 310 315 320
Ala Ser Pro Pro Pro Pro Gln Val Ala Ala Pro Glu Ala Ala Ser Pro
325 330 335
Pro Thr Gly Thr Asn Thr Ala Asn Thr Thr Ala Thr Phe Val Asn Glu
340 345 350
Ser Pro Ser Gln Lys Val Glu Thr Phe Asp Glu Phe Asp Pro Arg Ser
355 360 365
Ala Phe Ser Ala Gly Pro Pro Ala Tyr Ala Ser Thr Asp Gly Val Thr
370 375 380
Ala Pro Pro Thr Val Thr Ser Met Ser Ala Pro Thr Thr Ser Asn Ser
385 390 395 400
Val Glu Met Asp Leu Leu Gly Ser Leu Ala Asp Val Phe Ser Ser Asn
405 410 415
Ala Leu Ala Ile Val Pro Ala Asp Ser Ile Tyr Val Glu Thr Asn Gly
420 425 430
Gln Ala Asn Ala Gly Pro Ala Pro Ser Phe Ser Thr Ser Gln Pro Ser
435 440 445
Thr Gln Ser Phe Asp Asp Pro Phe Gly Asp Ser Pro Phe Lys Ala Phe
450 455 460
Thr Ser Thr Asp Thr Asp Ser Thr Pro Gln Gln Asn Phe Gly Ala Ser
465 470 475 480
Phe Gln Pro Pro Pro Pro Ala Phe Thr Ser Glu Val Ser His Pro Asp
485 490 495
Thr Ala His Asn Phe Gly Phe Gly Asp Ser Phe Ser Ala Val Ala Asn
500 505 510
Pro Asp Pro Ala Ser Gln Asn Val Gln Pro Pro Ser Asn Ser Pro Gly
515 520 525
Phe Pro Gln Glu Gln Phe Ala Thr Ser Gln Ser Gly Ile Asp Ile Leu
530 535 540
Ala Gly Ile Leu Pro Pro Ser Gly Pro Pro Val Gln Ser Gly Pro Ser
545 550 555 560
Ile Pro Thr Ser Gln Phe Pro Pro Ser Gly Asn Asn Met Tyr Glu Gly
565 570 575
Phe His Ser Gln Pro Pro Val Ser Thr Ala Pro Asn Leu Pro Gly Gln
580 585 590
Thr Pro Phe Gly Gln Ala Val Gln Pro Tyr Asn Met Val Pro His Ser
595 600 605
Gln Asn Met Thr Gly Ala Met Pro Phe Asn Ser Gly Gly Phe Met His
610 615 620
Gln Pro Gly Ser Gln Thr Pro Tyr Ser Thr Pro Ser Gly Pro Ala Gly
625 630 635 640
Gln Phe Met Ala Leu Ala
645
<210>83
<211>2688
<212>DNA
<213>拟南芥
<400>83
atgaagaaag tcttcggaca aactgttaga gaccttaaga gagaggtcaa caagaaagtg 60
ctcaaagttc ctggagtaga acagaaggtt ctagatgcta ctagtaatga gccatggggt 120
ccacatggat cacttcttgc tgatcttgcg caagcttcca gaaattatca tgaatatcag 180
ctgatcatgg tggtcatatg gaaacgcctt agcgacaccg gaaaaaactg gcggcatgtc 240
tataaggctt tgacagtttt ggagtacatg gtaggccatg gatcagaacg tgttatagac 300
gagattagag aacgtgcata tcaaatttcg acattgtccg attttcagta tattgattcc 360
ggtggtagag atcaaggaag caatgttagg aagaaatcac agagcctggt ggctttggtg 420
aatgacaagg aaagaatagc tgaagtcaga cagaaggctg ctgctaatag agataagtat 480
cgcagctcag caccaggtgg gatgtataag ccttcaggcg gatatgggga taaatatgat 540
tacggaagcc gggatgaaga gcgaagtagt tatggaagag aaagagaata tggttacaga 600
gatgatgata gaaatagtcg tgatggagat catcattcca gggactctga agaccggtat 660
gggagagatg gtaataggga tgatgattac aggggaagga gcagaagtgt tgataactat 720
gggtcacgag gtaggagttc ggaaagagag cgggaggatg atggccattc ttcatcacgg 780
ggcagtggtg ctcgagctga tgataattct caggatggga gaggggggct ccagaggaag 840
ttttctgaac aaaatattgg cgctccaccc agttatgaag aagctgtcag tgattcacgc 900
agccctgtat atagtgaaag ggatggtggg gagaccccac aagttaccgc tccaggagct 960
gcttctcctc ctcccccaca agttgctgct ccagaggctg cttctcctcc aactggaacc 1020
aacacagcca atacaactgc tacttttgtt aatgaatcac cttctcagaa agttgagact 1080
ttcgatgaat ttgatccacg cagtgcgttt tcagctggcc ctccagcata tgcatctaca 1140
gatggtgtta cagctcctcc aactgtgact tctatgtctg cccctaccac ctcaaacagt 1200
gttgagatgg acttgcttgg ttcccttgca gacgtatttt catcaaacgc attggccatt 1260
gtaccagctg attctatcta tgttgaaacc aatggacaag caaacgctgg tccagctcca 1320
tcgttttcta catctcagcc atcaactcag tcatttgatg acccatttgg tgactctcct 1380
ttcaaggcct tcacttctac tgacaccgac tcaaccccac agcagaattt tggagcttct 1440
ttccagccac cacctccagc cttcacttca gaggtctcac atcctgatac tgctcataac 1500
tttggctttg gggactcatt ttccgccgtt gccaatcctg atcctgcttc tcagaatgtg 1560
caacctccat cgaactcacc aggttttcct caagagcaat ttgctacatc tcagagtggt 1620
attgatatcc tcgctggcat tctcccacca tctggacctc cagtacaatc gggtccctca 1680
ataccaacat ctcaatttcc tcccagtgga aacaacatgt acgagggatt tcattctcag 1740
ccgccggtat ctacagctcc aaacctgcct ggacaaactc catttggaca agctgttcaa 1800
ccatataaca tggttcctca ttctcaaaat atgactggag ccatgccatt taatagtgga 1860
ggcttcatgc accagccggg ctcacaaact ccttactcta ccccaagtgg accagctggt 1920
cagttcatgg cacaccaagg tcatggaatg ccgccatccc atggtccaca acgaactcaa 1980
tccggacctg ttactctgca agggaacaac aatgtcatgg gagacatgtt ctcgcaagct 2040
acgcccaact ccttgacgtc atcatcatct catccagatc tcacaccttt aacaggagca 2100
attgagattg ttcctccgcc tcagaaaaag tttgagccaa aatcatcagt ctgggcagac 2160
acgttgagca gggggcttgt taactttaac atatctggat ctaaaacaaa tccattggca 2220
gacataggag ttgacttcga ggcgatcaac aggagagaga aacggctgga gaaacaaaca 2280
aacacaccag caacatcgac tatcaatatg ggtaaagcca tgggatcagg cactggctta 2340
gggcgttctg gtgcaactgc tatgagacct ccacctaatc caatgacagg ctctggcatg 2400
cccatgggcg gaggaatggg tgttggtagc tacggaggta tgaaccagaa ccaacccatg 2460
ggtatgggta tgggggccgg aatgaaccaa aaccaaccga tgggtatggg aatgggaccc 2520
ggcatgaaca tgaacatgaa catgggagga tatggccaag actatccgat gcaaccacaa 2580
aacccaggga tggtacctag cccaaacatg cctggcaata actacaatcc gatgatgggt 2640
caaggtggtt acaaccctca acaatcctat ggtggtggat accggtaa 2688
<210>84
<211>895
<212>PRT
<213>拟南芥
<400>84
Met Lys Lys Val Phe Gly Gln Thr Val Arg Asp Leu Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Val Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser Leu Leu Ala Asp
35 40 45
Leu Ala Gln Ala Ser Arg Asn Tyr His Glu Tyr Gln Leu Ile Met Val
50 55 60
Val Ile Trp Lys Arg Leu Ser Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Met Val Gly His Gly Ser Glu
85 90 95
Arg Val Ile Asp Glu Ile Arg Glu Arg Ala Tyr Gln Ile Ser Thr Leu
100 105 110
Ser Asp Phe Gln Tyr Ile Asp Ser Gly Gly Arg Asp Gln Gly Ser Asn
115 120 125
Val Arg Lys Lys Ser Gln Ser Leu Val Ala Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Ala Glu Val Arg Gln Lys Ala Ala Ala Asn Arg Asp Lys Tyr
145 150 155 160
Arg Ser Ser Ala Pro Gly Gly Met Tyr Lys Pro Ser Gly Gly Tyr Gly
165 170 175
Asp Lys Tyr Asp Tyr Gly Ser Arg Asp Glu Glu Arg Ser Ser Tyr Gly
180 185 190
Arg Glu Arg Glu Tyr Gly Tyr Arg Asp Asp Asp ArgAsn Ser Arg Asp
195 200 205
Gly Asp His His Ser Arg Asp Ser Glu Asp Arg Tyr Gly Arg Asp Gly
210 215 220
Asn Arg Asp Asp Asp Tyr Arg Gly Arg Ser Arg Ser Val Asp Asn Tyr
225 230 235 240
Gly Ser Arg Gly Arg Ser Ser Glu Arg Glu Arg Glu Asp Asp Gly His
245 250 255
Ser Ser Ser Arg Gly Ser Gly Ala Arg Ala Asp Asp Asn Ser Gln Asp
260 265 270
Gly Arg Gly Gly Leu Gln Arg Lys Phe Ser Glu Gln Asn Ile Gly Ala
275 280 285
Pro Pro Ser Tyr Glu Glu Ala Val Ser Asp Ser Arg Ser Pro Val Tyr
290 295 300
Ser Glu Arg Asp Gly Gly Glu Thr Pro Gln Val Thr Ala Pro Gly Ala
305 310 315 320
Ala Ser Pro Pro Pro Pro Gln Val Ala Ala Pro Glu Ala Ala Ser Pro
325 330 335
Pro Thr Gly Thr Asn Thr Ala Asn Thr Thr Ala Thr Phe Val Asn Glu
340 345 350
Ser Pro Ser Gln Lys Val Glu Thr Phe Asp Glu Phe Asp Pro Arg Ser
355 360 365
Ala Phe Ser Ala Gly Pro Pro Ala Tyr Ala Ser Thr Asp Gly Val Thr
370 375 380
Ala Pro Pro Thr Val Thr Ser Met Ser Ala Pro Thr Thr Ser Asn Ser
385 390 395 400
Val Glu Met Asp Leu Leu Gly Ser Leu Ala Asp Val Phe Ser Ser Asn
405 410 415
Ala Leu Ala Ile Val Pro Ala Asp Ser Ile Tyr Val Glu Thr Asn Gly
420 425 430
Gln Ala Asn Ala Gly Pro Ala Pro Ser Phe Ser Thr Ser Gln Pro Ser
435 440 445
Thr Gln Ser Phe Asp Asp Pro Phe Gly Asp Ser Pro Phe Lys Ala Phe
450 455 460
Thr Ser Thr Asp Thr Asp Ser Thr Pro Gln Gln Asn Phe Gly Ala Ser
465 470 475 480
Phe Gln Pro Pro Pro Pro Ala Phe Thr Ser Glu Val Ser His Pro Asp
485 490 495
Thr Ala His Asn Phe Gly Phe Gly Asp Ser Phe Ser Ala Val Ala Asn
500 505 510
Pro Asp Pro Ala Ser Gln Asn Val Gln Pro Pro Ser Asn Ser Pro Gly
515 520 525
Phe Pro Gln Glu Gln Phe Ala Thr Ser Gln Ser Gly Ile Asp Ile Leu
530 535 540
Ala Gly Ile Leu Pro Pro Ser Gly Pro Pro Val Gln Ser Gly Pro Ser
545 550 555 560
Ile Pro Thr Ser Gln Phe Pro Pro Ser Gly Asn Asn Met Tyr Glu Gly
565 570 575
Phe His Ser Gln Pro Pro Val Ser Thr Ala Pro Asn Leu Pro Gly Gln
580 585 590
Thr Pro Phe Gly Gln Ala Val Gln Pro Tyr Asn Met Val Pro His Ser
595 600 605
Gln Asn Met Thr Gly Ala Met Pro Phe Asn Ser Gly Gly Phe Met His
610 615 620
Gln Pro Gly Ser Gln Thr Pro Tyr Ser Thr Pro Ser Gly Pro Ala Gly
625 630 635 640
Gln Phe Met Ala His Gln Gly His Gly Met Pro Pro Ser His Gly Pro
645 650 655
Gln Arg Thr Gln Ser Gly Pro Val Thr Leu Gln Gly Asn Asn Asn Val
660 665 670
Met Gly Asp Met Phe Ser Gln Ala Thr Pro Asn Ser Leu Thr Ser Ser
675 680 685
Ser Ser His Pro Asp Leu Thr Pro Leu Thr Gly Ala Ile Glu Ile Val
690 695 700
Pro Pro Pro Gln Lys Lys Phe Glu Pro Lys Ser Ser Val Trp Ala Asp
705 710 715 720
Thr Leu Ser Arg Gly Leu Val Asn Phe Asn Ile Ser Gly Ser Lys Thr
725 730 735
Asn Pro Leu Ala Asp Ile Gly Val Asp Phe Glu Ala Ile Asn Arg Arg
740 745 750
Glu Lys Arg Leu Glu Lys Gln Thr Asn Thr Pro Ala Thr Ser Thr Ile
755 760 765
Asn Met Gly Lys Ala Met Gly Ser Gly Thr Gly Leu Gly Arg Ser Gly
770 775 780
Ala Thr Ala Met Arg Pro Pro Pro Asn Pro Met Thr Gly Ser Gly Met
785 790 795 800
Pro Met Gly Gly Gly Met Gly Val Gly Ser Tyr Gly Gly Met Asn Gln
805 810 815
Asn Gln Pro Met Gly Met Gly Met Gly Ala Gly Met Asn Gln Asn Gln
820 825 830
Pro Met Gly Met Gly Met Gly Pro Gly Met Asn Met Asn Met Asn Met
835 840 845
Gly Gly Tyr Gly Gln Asp Tyr Pro Met Gln Pro Gln Asn Pro Gly Met
850 855 860
Val Pro Ser Pro Asn Met Pro Gly Asn Asn Tyr Asn Pro Met Met Gly
865 870 875 880
Gln Gly Gly Tyr Asn Pro Gln Gln Ser Tyr Gly Gly Gly Tyr Arg
885 890 895
<210>85
<211>2838
<212>DNA
<213>稻
<400>85
atgaagaaag tattcgatca aacagtccgg gacctaaaaa gagaggtgaa caagaaggtc 60
ctcaaggtgc ctggtataga acaaaagatt cttgatgcca caagcaacga gccatggggc 120
ccacatggat cactcttggc agaaatcgcc caagcaacac aaaattacca tgagtaccag 180
atggtcatga atgttgtgtg gaagaggatc aatgacactg gtaaaaattg gcggcatgtt 240
tataagggat tgattgtcct ggattacttg gttgctcatg gtacagagcg agttattgat 300
gacataaggg aacattctta tcagatatcg acactagctg atttccagta tatcgattcg 360
agtgggagag atcaaggtag caatgtacgg cggaaatccc agagccttgt tagtttagtt 420
aatgacaaag agaggataca ggaagttagg cagaaagcac ttgctaccag ggacaaatac 480
cggagtgcat tcgccaccag tggaacacac aggagcccag gtggctatga caatgaccgc 540
tatgaaggaa gctatggcag taggtatgat aacagaaatg gctacggggg agaaagagag 600
tatggatata gggatgatga cagatatggt gttgctggaa ctactcccaa tcgggaagga 660
gatcgttatt ctagggattc taatgagcag cgctacagca gagatagaga agatgagtac 720
aaaggaagtc atagcaacca tgaatatgca gaaggatcag gtcgcaggag ctatggacga 780
gatagggatt catatgggga tgatgaagct tattcatccc gtggccggca aagcaatgct 840
gacggaccta ctcaggatga gaggcccatg gagcggaagc cttctaacca gcagattgct 900
tcaccaccgc caaactatga agatgtcaca agagatacac aggataataa tcatgatgga 960
cgaaatggag ggactgtgcc tgtgccagtt gctgcagcaa aggtgtcttc acctccaaga 1020
acaagtgtac ccccaggtca ggtgaatggt gtacatgata atactgtgga agacgtacct 1080
gcaccaccac ctactcatcc tgaagttaat gggtttgacg agtttgatcc tcgtggatca 1140
gtaccagata cctcacctcc agtgaacccc tcacaagccg tgaatagctt ggaaatggat 1200
ttattcggac cagatcctat taattcgttg gctttggtat ctgtgcctca gccaactgcc 1260
agcccaaatg ttgagccatc agcaaatcca ggatttgaga gtaacagttt tatgggcatg 1320
ccaccggctt ctactggatt caatgaggcc tttgatgcaa ctaatccttt tggggatcct 1380
acgcctttca aggctgttca tgaagaaact cctgcagttt ctcagacaaa tgctgcacct 1440
gctggttctt tccatgctac tgaacctgct gcagatgcaa atcctttcca gcctgcttca 1500
gcagcaagct ttggttttgg agacactctt ggcgacctct cttttggatc gaatgctgca 1560
cccgggcagc aagatatttt tgtgcccacc tcatcacatt cagaggtacc acctgcaaac 1620
ccatctgtgc accctgaaca ggcagtccca tcttatgtct cctcccaggc acctcagcct 1680
gcagctgctg gtccacaaac tcatgctgct cccgcttcat ttgcttctca agcacctccc 1740
acttcatttg cttctcaagc acctcaagca ggggcaccct atcctcaggc tgcctccaca 1800
tttcctcatt cacaagcatc tcatcctgca gccactaatc catctacaat ccctcagaat 1860
gttgccacac catttgctcc tttacaaatg cctcaacctg taccatcagg ccaatcaaac 1920
tattttatgc aaccagttcc agggactggc attaatggca tgtctggggc tccttcacag 1980
aatggagcac catcctacat cccttcacag gcttctcaat ttgcagctcc aacaaatcta 2040
cagccctctc agccaacctt ccccccacaa actgccatgg cagcttcaca ggcaacatca 2100
atttctcgag gggcatctca gcctcttgca gtgccgaatt caatgccttc tggtgtgaac 2160
tttccattac aatcaagttc atcagctccc cctgaaacaa tcctttcagc cttgcaagtt 2220
agtcagtctg agccagtgaa gaaatttgag tccaaatcta cagtttgggc tgatacattg 2280
agccgaggtt tagtcaatct ggacatctct ggaccaaagg ctaatccgca cgctgatatt 2340
ggagttgact tcgattcaat caatcgcaag gagaaaaggc aagaaaagaa agtctctcaa 2400
gctcctgtgg tatctacgat cactatgggc aaagccatgg gaactggctc cggcatcggt 2460
cgggcaggtg cgagtgccat ggcacctcca gccggcccaa tgggtgcaag caggggcatt 2520
ggcatgggta tgggcgctgc tggttccggt tacggtggtg gaatgggaat gaaccggcca 2580
atgggcatgg gaatgggaat gaaccaacaa atgggtatgg gaatggggat gaaccaacag 2640
gccatgggaa tggggatgaa ccaacaggcc atgggaatgg ggatgaacca acaaccaatg 2700
ggaatgaaca tgggcatggg gatgaaccag ggtatgggaa tgaacatgcg gcctcctatg 2760
ggaatgggtc caggtagtgg atataatcca atgggtaccg gatacggagg gcagcaacca 2820
tatggtgggt acaggtga 2838
<210>86
<211>945
<212>PRT
<213>稻
<400>86
Met Lys Lys Val Phe Asp Gln Thr Val Arg Asp Leu Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Ile Glu Gln Lys Ile Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser Leu Leu Ala Glu
35 40 45
Ile Ala Gln Ala Thr Gln Asn Tyr His Glu Tyr Gln Met Val Met Asn
50 55 60
Val Val Trp Lys Arg Ile Asn Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Gly Leu Ile Val Leu Asp Tyr Leu Val Ala His Gly Thr Glu
85 90 95
Arg Val Ile Asp Asp Ile Arg Glu His Ser Tyr Gln Ile Ser Thr Leu
100 105 110
Ala Asp Phe Gln Tyr Ile Asp Ser Ser Gly Arg Asp Gln Gly Ser Asn
115 120 125
Val Arg Arg Lys Ser Gln Ser Leu Val Ser Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Gln Glu Val Arg Gln Lys Ala Leu Ala Thr Arg Asp Lys Tyr
145 150 155 160
Arg Ser Ala Phe Ala Thr Ser Gly Thr His Arg Ser Pro Gly Gly Tyr
165 170 175
Asp Asn Asp Arg Tyr Glu Gly Ser Tyr Gly Ser Arg Tyr Asp Asn Arg
180 185 190
Asn Gly Tyr Gly Gly Glu Arg Glu Tyr Gly Tyr Arg Asp Asp Asp Arg
195 200 205
Tyr Gly Val Ala Gly Thr Thr Pro Asn Arg Glu Gly Asp Arg Tyr Ser
210 215 220
Arg Asp Ser Asn Glu Gln Arg Tyr Ser Arg Asp Arg Glu Asp Glu Tyr
225 230 235 240
Lys Gly Ser His Ser Asn His Glu Tyr Ala Glu Gly Ser Gly Arg Arg
245 250 255
Ser Tyr Gly Arg Asp Arg Asp Ser Tyr Gly Asp Asp Glu Ala Tyr Ser
260 265 270
Ser Arg Gly Arg Gln Ser Asn Ala Asp Gly Pro Thr Gln Asp Glu Arg
275 280 285
Pro Met Glu Arg Lys Pro Ser Asn Gln Gln Ile Ala Ser Pro Pro Pro
290 295 300
Asn Tyr Glu Asp Val Thr Arg Asp Thr Gln Asp Asn Asn His Asp Gly
305 310 315 320
Arg Asn Gly Gly Thr Val Pro Val Pro Val Ala Ala Ala Lys Val Ser
325 330 335
Ser Pro Pro Arg Thr Ser Val Pro Pro Gly Gln Val Asn Gly Val His
340 345 350
Asp Asn Thr Val Glu Asp Val Pro Ala Pro Pro Pro Thr His Pro Glu
355 360 365
Val Asn Gly Phe Asp Glu Phe Asp Pro Arg Gly Ser Val Pro Asp Thr
370 375 380
Ser Pro Pro Val Asn Pro Ser Gln Ala Val Asn Ser Leu Glu Met Asp
385 390 395 400
Leu Phe Gly Pro Asp Pro Ile Asn Ser Leu Ala Leu Val Ser Val Pro
405 410 415
Gln Pro Thr Ala Ser Pro Asn Val Glu Pro Ser Ala Asn Pro Gly Phe
420 425 430
Glu Ser Asn Ser Phe Met Gly Met Pro Pro Ala Ser Thr Gly Phe Asn
435 440 445
Glu Ala Phe Asp Ala Thr Asn Pro Phe Gly Asp Pro Thr Pro Phe Lys
450 455 460
Ala Val His Glu Glu Thr Pro Ala Val Ser Gln Thr Asn Ala Ala Pro
465 470 475 480
Ala Gly Ser Phe His Ala Thr Glu Pro Ala Ala Asp Ala Asn Pro Phe
485 490 495
Gln Pro Ala Ser Ala Ala Ser Phe Gly Phe Gly Asp Thr Leu Gly Asp
500 505 510
Leu Ser Phe Gly Ser Asn Ala Ala Pro Gly Gln Gln Asp Ile Phe Val
515 520 525
Pro Thr Ser Ser His Ser Glu Val Pro Pro Ala Asn Pro Ser Val His
530 535 540
Pro Glu Gln Ala Val Pro Ser Tyr Val Ser Ser Gln Ala Pro Gln Pro
545 550 555 560
Ala Ala Ala Gly Pro Gln Thr His Ala Ala Pro Ala Ser Phe Ala Ser
565 570 575
Gln Ala Pro Pro Thr Ser Phe Ala Ser Gln Ala Pro Gln Ala Gly Ala
580 585 590
Pro Tyr Pro Gln Ala Ala Ser Thr Phe Pro His Ser Gln Ala Ser His
595 600 605
Pro Ala Ala Thr Asn Pro Ser Thr Ile Pro Gln Asn Val Ala Thr Pro
610 615 620
Phe Ala Pro Leu Gln Met Pro Gln Pro Val Pro Ser Gly Gln Ser Asn
625 630 635 640
Tyr Phe Met Gln Pro Val Pro Gly Thr Gly Ile Asn Gly Met Ser Gly
645 650 655
Ala Pro Ser Gln Asn Gly Ala Pro Ser Tyr Ile Pro Ser Gln Ala Ser
660 665 670
Gln Phe Ala Ala Pro Thr Asn Leu Gln Pro Ser Gln Pro Thr Phe Pro
675 680 685
Pro Gln Thr Ala Met Ala Ala Ser Gln Ala Thr Ser Ile Ser Arg Gly
690 695 700
Ala Ser Gln Pro Leu Ala Val Pro Asn Ser Met Pro Ser Gly Val Asn
705 710 715 720
Phe Pro Leu Gln Ser Ser Ser Ser Ala Pro Pro Glu Thr Ile Leu Ser
725 730 735
Ala Leu Gln Val Ser Gln Ser Glu Pro Val Lys Lys Phe Glu Ser Lys
740 745 750
Ser Thr Val Trp Ala Asp Thr Leu Ser Arg Gly Leu Val Asn Leu Asp
755 760 765
Ile Ser Gly Pro Lys Ala Asn Pro His Ala Asp Ile Gly Val Asp Phe
770 775 780
Asp Ser Ile Asn Arg Lys Glu Lys Arg Gln Glu Lys Lys Val Ser Gln
785 790 795 800
Ala Pro Val Val Ser Thr Ile Thr Met Gly Lys Ala Met Gly Thr Gly
805 810 815
Ser Gly Ile Gly Arg Ala Gly Ala Ser Ala Met Ala Pro Pro Ala Asn
820 825 830
Pro Met Gly Ala Ser Arg Gly Ile Gly Met Gly Met Gly Ala Ala Gly
835 840 845
Ser Gly Tyr Gly Gly Gly Met Gly Met Asn Arg Pro Met Gly Met Gly
850 855 860
Met Gly Met Asn Gln Gln Met Gly Met Gly Met Gly Met Asn Gln Gln
865 870 875 880
Ala Met Gly Met Gly Met Asn Gln Gln Ala Met Gly Met Gly Met Asn
885 890 895
Gln Gln Pro Met Gly Met Asn Met Gly Met Gly Met Asn Gln Gly Met
900 905 910
Gly Met Asn Met Arg Pro Pro Met Gly Met Gly Pro Gly Ser Gly Tyr
915 920 925
Asn Pro Met Gly Thr Gly Tyr Gly Gly Gln Gln Pro Tyr Gly Gly Tyr
930 935 940
Arg
945
<210>87
<211>3072
<212>DNA
<213>拟南芥
<400>87
atgaagaaag catttggtca aactgtcaga gacctcaaga gaggggtcaa caagaaagtg 60
cttaaagtac ctggaataga acagaaggtt ctagatgcta ccagcaatga gtcctggggt 120
ccgcatggat cacttcttgc ggacattgca catgcttcga gaaattacca tgaataccag 180
attactatgg gagtgttatg gaaacgtctt agtgactctg gaaaaaattg gcggcatgtc 240
tataaggcat tgacagtctt ggagtacatg gtaggccatg gatcagaacg tgtcatagaa 300
gaggttaaag aacatgcata tcaaattacg acattatctg gttttcagta tattgattcc 360
agcggtaaag atcaaggtag caatgtcagg aagaaggcgc agagccttgt ggctttggtt 420
aatgacaaag aaagaataac ggaggtcaga gagaaggctg ctgctaacag agataagtat 480
cataactcaa tgcataggcc gtcaggagga tatggggaca aatatgatta cgaaggccgc 540
tatggagaca gagatgaagg gcgaagtagt tatgggaaag agagagaata tggttacagg 600
gatgatgata gaaatagtcg tgatggcgat cgttattcta gagactctga agaccgatat 660
ggaagagatg gtaacacgga tgacgaatac cgtggaagga gcagaagtgt tgataattac 720
aatggatcaa gaggtaggag ctcggacagg gaacggccta ttgaggatga tggccaatcc 780
tcatcgcggg atagtggtgc gccagctgat gatcattctc aagatgggag aggggggcta 840
gagagaaagt tctctgaaca aaatattggt gctgctccac ctagttatga agaagctgtg 900
agtgaatcac ggagccctgt atacagtgaa agggatggtg gagagacccc acaagttgct 960
cctccaggag ctgctgcttc tcctcttgct gaaaacatca gcgtggacaa caaagctgct 1020
gattttgtta atgaatcatc tcctcagcaa gttgaggctt ttgatgaatt tgatccacga 1080
ggttcagttt cagcatgtgc acctacagct ggtgcttcgg ttcctgcacc tatcccacca 1140
acagtggttt ctacgcctgc cccgcctgcc tcaattaatg cagaaatgga tttgcttggc 1200
tctctttcag atgtattttc accaaatccc ttggctattg taacatctga ttccacttct 1260
gttgaaacca atggacaagc aaacactggt ttagctccat cgttttctac ttctcagtca 1320
tctactcagc cttttgatga tccatttggt gactctcctt tcaaagccat cacttctgct 1380
gacactgaga caagccaaca tcagagtttt ggagttcctt ttcagccaac accaccaact 1440
tcaaatccta acaatgaaca taactttggt tttggggaag cattttcagc tgttaccgat 1500
tctgaacctg gcgttcaaaa tatgcaagct ccaccgaact tatccgtctt tcctcaagag 1560
cagtttgata catcgcagag cgaaatcgat attcttgccg gcattctccc accatctgga 1620
ccgccagttt ctctgtctcc acaaccagat tccactatgc caacatccca atttcatccc 1680
aatggtaact cctatgaaag ctatcatcat caagccgcgc ctacagatct aaacatgcaa 1740
ggacaaacac cgtttggtca agcttcacaa caatttaaca tggtttcaca ttcacaaaat 1800
catcatgaag gtatgcaatt caacaatggg ggcttcacgc aacagccagg ctatgcaggt 1860
ccagcaacct ctcaacctcc acaatatact cctggtgtat cttcacaccc accaagtgag 1920
agttttcctc accaaccagg ttctgccacc tcagcaagct cgcaaactcc ttacgctact 1980
acacccaatg tatcagctgg gcagtttgat ggtgggagtt tcatgacaca gcaaccttat 2040
ggtgtgacac aacaagttca tgttgtccct tcccatatac cacagcgaac tcaatctgga 2100
cctgttgcag cgtttgggaa taataacaat atcgttggag atatgcacca acctggttct 2160
accccctcat caagctcgca aactccttac cctactacgc ccaatgcacc aagtggtcag 2220
tttgatggtg gaaattttat gacacaacaa ccttatggtg tgataccgca agttcatggc 2280
gtcccttccc atataccaca gcgaactcaa tctggacctg ttgcagcgca cgggaatagt 2340
aacaatgttg ttggggatat gttttcacca gctggactaa gttccttgga gacatcagca 2400
tcgcaaccat ctctcacacc tttaacagga gcaattgaaa ttgttcccca aaatcagaag 2460
aagtttgagc caaaatcaac aatttgggct gatacattaa gtagagggct agttaacttc 2520
aacatttctg gaccgaaaac aaatccattg gcggatatag gagttgactt cgaggcaatc 2580
aacaggaaag agaaacggct agagaaacca actatcacac aacaacaagt aacatcaact 2640
atcaacatgg ggaaagccat gggatctggt actggcttag gtcgcgctgg tgcaggtgct 2700
atgagacctc caacaaattc aatggtaggc tcaagcatgc ccacgggcat gaatgttggt 2760
ggctatggag gcatgaacca acaccaaccc ataggcatga accaaaacca tcccatgggc 2820
atgaaccaaa acctttccat gggaatgaac cagaactatc ccatgggaat gaaccaaaac 2880
tatccaatgg gaatgggcat gaacatgaac atgggcgggt atggacaagg gtatccgatg 2940
caaccacaac aaggaatggg catggctcct cctggtgcac cacaaggcat gaccggtgcc 3000
tataatccaa tgatgggtca aggcggttac aaccctcagc agcagcaacc atatggtgga 3060
ggttaccggt aa 3072
<210>88
<211>1023
<212>PRT
<213>拟南芥
<400>88
Met Lys Lys Ala Phe Gly Gln Thr Val Arg Asp Leu Lys Arg Gly Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Ile Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Ser Trp Gly Pro His Gly Ser Leu Leu Ala Asp
35 40 45
Ile Ala His Ala Ser Arg Asn Tyr His Glu Tyr Gln Ile Thr Met Gly
50 55 60
Val Leu Trp Lys Arg Leu Ser Asp Ser Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Met Val Gly His Gly Ser Glu
85 90 95
Arg Val Ile Glu Glu Val Lys Glu His Ala Tyr Gln Ile Thr Thr Leu
100 105 110
Ser Gly Phe Gln Tyr Ile Asp Ser Ser Gly Lys Asp Gln Gly Ser Asn
115 120 125
Val Arg Lys Lys Ala Gln Ser Leu Val Ala Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Thr Glu Val Arg Glu Lys Ala Ala Ala Asn Arg Asp Lys Tyr
145 150 155 160
His Asn Ser Met His Arg Pro Ser Gly Gly Tyr Gly Asp Lys Tyr Asp
165 170 175
Tyr Glu Gly Arg Tyr Gly Asp Arg Asp Glu Gly Arg Ser Ser Tyr Gly
180 185 190
Lys Glu Arg Glu Tyr Gly Tyr Arg Asp Asp Asp Arg Asn Ser Arg Asp
195 200 205
Gly Asp Arg Tyr Ser Arg Asp Ser Glu Asp Arg Tyr Gly Arg Asp Gly
210 215 220
Asn Thr Asp Asp Glu Tyr Arg Gly Arg Ser Arg Ser Val Asp Asn Tyr
225 230 235 240
Asn Gly Ser Arg Gly Arg Ser Ser Asp Arg Glu Arg Pro Ile Glu Asp
245 250 255
Asp Gly Gln Ser Ser Ser Arg Asp Ser Gly Ala Pro Ala Asp Asp His
260 265 270
Ser Gln Asp Gly Arg Gly Gly Leu Glu Arg Lys Phe Ser Glu Gln Asn
275 280 285
Ile Gly Ala Ala Pro Pro Ser Tyr Glu Glu Ala Val Ser Glu Ser Arg
290 295 300
Ser Pro Val Tyr Ser Glu Arg Asp Gly Gly Glu Thr Pro Gln Val Ala
305 310 315 320
Pro Pro Gly Ala Ala Ala Ser Pro Leu Ala Glu Asn Ile Ser Val Asp
325 330 335
Asn Lys Ala Ala Asp Phe Val Asn Glu Ser Ser Pro Gln Gln Val Glu
340 345 350
Ala Phe Asp Glu Phe Asp Pro Arg Gly Ser Val Ser Ala Cys Ala Pro
355 360 365
Thr Ala Gly Ala Ser Val Pro Ala Pro Ile Pro Pro Thr Val Val Ser
370 375 380
Thr Pro Ala Pro Pro Ala Ser Ile Asn Ala Glu Met Asp Leu Leu Gly
385 390 395 400
Ser Leu Ser Asp Val Phe Ser Pro Asn Pro Leu Ala Ile Val Thr Ser
405 410 415
Asp Ser Thr Ser Val Glu Thr Asn Gly Gln Ala Asn Thr Gly Leu Ala
420 425 430
Pro Ser Phe Ser Thr Ser Gln Ser Ser Thr Gln Pro Phe Asp Asp Pro
435 440 445
Phe Gly Asp Ser Pro Phe Lys Ala Ile Thr Ser Ala Asp Thr Glu Thr
450 455 460
Ser Gln His Gln Ser Phe Gly Val Pro Phe Gln Pro Thr Pro Pro Thr
465 470 475 480
Ser Asn Pro Asn Asn Glu His Asn Phe Gly Phe Gly Glu Ala Phe Ser
485 490 495
Ala Val Thr Asp Ser Glu Pro Gly Val Gln Asn Met Gln Ala Pro Pro
500 505 510
Asn Leu Ser Val Phe Pro Gln Glu Gln Phe Asp Thr Ser Gln Ser Glu
515 520 525
Ile Asp Ile Leu Ala Gly Ile Leu Pro Pro Ser Gly Pro Pro Val Ser
530 535 540
Leu Ser Pro Gln Pro Asp Ser Thr Met Pro Thr Ser Gln Phe His Pro
545 550 555 560
Asn Gly Asn Ser Tyr Glu Ser Tyr His His Gln Ala Ala Pro Thr Asp
565 570 575
Leu Asn Met Gln Gly Gln Thr Pro Phe Gly Gln Ala Ser Gln Gln Phe
580 585 590
Asn Met Val Ser His Ser Gln Asn His His Glu Gly Met Gln Phe Asn
595 600 605
Asn Gly Gly Phe Thr Gln Gln Pro Gly Tyr Ala Gly Pro Ala Thr Ser
610 615 620
Gln Pro Pro Gln Tyr Thr Pro Gly Val Ser Ser His Pro Pro Ser Glu
625 630 635 640
Ser Phe Pro His Gln Pro Gly Ser Ala Thr Ser Ala Ser Ser Gln Thr
645 650 655
Pro Tyr Ala Thr Thr Pro Asn Val Ser Ala Gly Gln Phe Asp Gly Gly
660 665 670
Ser Phe Met Thr Gln Gln Pro Tyr Gly Val Thr Gln Gln Val His Val
675 680 685
Val Pro Ser His Ile Pro Gln Arg Thr Gln Ser Gly Pro Val Ala Ala
690 695 700
Phe Gly Asn Asn Asn Asn Ile Val Gly Asp Met His Gln Pro Gly Ser
705 710 715 720
Thr Pro Ser Ser Ser Ser Gln Thr Pro Tyr Pro Thr Thr Pro Asn Ala
725 730 735
Pro Ser Gly Gln Phe Asp Gly Gly Asn Phe Met Thr Gln Gln Pro Tyr
740 745 750
Gly Val Ile Pro Gln Val His Gly Val Pro Ser His Ile Pro Gln Arg
755 760 765
Thr Gln Ser Gly Pro Val Ala Ala His Gly Asn Ser Asn Asn Val Val
770 775 780
Gly Asp Met Phe Ser Pro Ala Gly Leu Ser Ser Leu Glu Thr Ser Ala
785 790 795 800
Ser Gln Pro Ser Leu Thr Pro Leu Thr Gly Ala Ile Glu Ile Val Pro
805 810 815
Gln Asn Gln Lys Lys Phe Glu Pro Lys Ser Thr Ile Trp Ala Asp Thr
820 825 830
Leu Ser Arg Gly Leu Val Asn Phe Asn Ile Ser Gly Pro Lys Thr Asn
835 840 845
Pro Leu Ala Asp Ile Gly Val Asp Phe Glu Ala Ile Asn Arg Lys Glu
850 855 860
Lys Arg Leu Glu Lys Pro Thr Ile Thr Gln Gln Gln Val Thr Ser Thr
865 870 875 880
Ile Asn Met Gly Lys Ala Met Gly Ser Gly Thr Gly Leu Gly Arg Ala
885 890 895
Gly Ala Gly Ala Met Arg Pro Pro Thr Asn Ser Met Val Gly Ser Ser
900 905 910
Met Pro Thr Gly Met Asn Val Gly Gly Tyr Gly Gly Met Asn Gln His
915 920 925
Gln Pro Ile Gly Met Asn Gln Asn His Pro Met Gly Met Asn Gln Asn
930 935 940
Leu Ser Met Gly Met Asn Gln Asn Tyr Pro Met Gly Met Asn Gln Asn
945 950 955 960
Tyr Pro Met Gly Met Gly Met Asn Met Asn Met Gly Gly Tyr Gly Gln
965 970 975
Gly Tyr Pro Met Gln Pro Gln Gln Gly Met Gly Met Ala Pro Pro Gly
980 985 990
Ala Pro Gln Gly Met Thr Gly Ala Tyr Asn Pro Met Met Gly Gln Gly
995 1000 1005
Gly Tyr Asn Pro Gln Gln Gln Gln Pro Tyr Gly Gly Gly Tyr Arg
1010 1015 1020
<210>89
<211>2664
<212>DNA
<213>葡萄
<400>89
atgaagaagg ccattggtca aactgttagg gaccttaaga gagaggtgaa taagaaagtt 60
cttaaagttc caggaataga acagaaggtt ctggatgcta ctagcaatga gccttggggt 120
cctcatggaa cacatcttgc agatattgca caggctacca gaaactatca cgagtaccaa 180
atgatcatgt cagtaatctg gaagcggatt aatgatacag gaaagaattg gcggcatgtc 240
tacaaggctt tgacagttct ggaatacttg gtaggccatg gttctgagcg tgtcatagat 300
gagattcggg agcatatata ccaaatatca actttgtcag actttcaata tattgattca 360
agtgggaggg atcagggaag caatgtcaga aagaaatctc aaagtctcgt ggctctggtg 420
aatgataaag aaaggataca ggaagttaga cagaaggctg ctgccaacag ggacaagttt 480
cgcaacacaa actcagcagg cggtatgtat aggcccagtt catattctag ttcaggagga 540
tatggtgacc gttatgatga tgatcgatat gaaggccgct atgggagaga cgaagatcga 600
aatggttatg ggagagaaag agaatggggc agcagagatg atgaccggta tggtagaaat 660
ggggactcat atgagattct gatgaacggt atggtagaga tggctataag gatgatgatt 720
acaggggaag aagtcgaagc aatgaggact accagtatgg ctctagaagt aggagtgctg 780
atagagatag ggaccgagag caaccattca tctagaggtg gtgcccgaac tgatgagcat 840
cctcagtatg gaaggcagct tgaacgaaaa ttttctgaac aaaatcttga tgctcctcct 900
agttatgaag aagctgtagc tgatgcccac agtcctgttc atgatgagag ggatggagca 960
accccagcag cacctgcccc taaaacatct tctccacctg taagcactag tcctagccaa 1020
gcaacaactg ctgttggtcc ttctacatct cctcctgcca acaaggaagt tgatgctttt 1080
gatgaatttg atccgcgcgg tccagtctca ggtatggaga atcctagaga tttgtctgtt 1140
tgccagtatg tgcagcttga gtccaaacaa tttgcttgcg cttctgtccc agctacatca 1200
attagtccgg aaatggactt acttggctct ctgtcagagt cattttcttc aaattcattg 1260
gctcttgtgc catctggacc tgcaaccaca acatctgagg ctgctgtcct tggaaatgct 1320
gggtctgcac ccgcatcagc agcaatgcca tctggatctg cttcttttga agacccattt 1380
ggtgactccc cttttagagc tttgccttca gcagagagtg taccagctga acctcaggat 1440
tcagcttcca caacttcctt ccagaccatg aatcaaacat caggaccacc cttcccagtt 1500
acccaaggag tggacacggg ttccaacttt gactttgggg acactttccc tggcataact 1560
tacacgccat ccggtgtctc cactgcacaa cctccttctg caagcccaca attttcaccc 1620
caagagcaat ggattcccca gcagaacaat gatatcctag cagacattct accaccttca 1680
ggatcttcag ctcctgccat ttcacaggct gctttcccag ctccaactgg ccagtctgtg 1740
cagccaaacc ctaatgttgt tgggggcttt cttgcacagt caggatctgc agatcatgca 1800
gcttcacaat tttctcctca gattccaact ggaccagctg cacaatataa caatgggaac 1860
ttcctttcac atgggccagt tacacatcct agcaatgata accttggtgg cttatttcca 1920
caagctggac cgcctggttc agtgatgtca cagtctactc cccctgcttc aacaggttca 1980
cttgctatag ttcctcaagc atccaaggac aagtttgaaa cgaagtcaac ggtttgggct 2040
gatacactga gcagaggatt agtcaatttg aatatatctg gagctaaaat taatccattg 2100
gcggatattg gaattgattt tgatgccatt aatcggaaag aaaagaggat ggagaaacct 2160
aagcctgcta ccacagctcc tgtatctact actacaatgg gcaaggcaat gggatctggc 2220
tctggaatgg gtcgtgctgg tgcaggtgca ctcaggcctc caccaaatac tatgatgggt 2280
tcaggtatgg gaatgggcat gggcatgggt agtggccctg gtgcaggcat gagtatgggt 2340
atgggtggtg gtcctggttc aggcatgggt atgggtatgg gtggtgcccc tggagtgggc 2400
atgggtatgg gtatgggtgg tgcccctgca gcagcgggta tgggtatggg tatggggatg 2460
ggaggaggca tgggcatggg aggatatgga ggcatgaatc aacctatggg catgggtatg 2520
aatgtgggca tgggcatgaa tatgggaatg gggcaaggag cccagatgca acaaccaact 2580
ggattgcctc ctgggggtta taaccccatg atgggctcag gtggttatgc tcctcagcaa 2640
ccatatggtg gtggctaccg atga 2664
<210>90
<211>887
<212>PRT
<213>葡萄
<400>90
Met Lys Lys Ala Ile Gly Gln Thr Val Arg Asp Leu Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Ile Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Thr His Leu Ala Asp
35 40 45
Ile Ala Gln Ala Thr Arg Asn Tyr His Glu Tyr Gln Met Ile Met Ser
50 55 60
Val Ile Trp Lys Arg Ile Asn Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Leu Val Gly His Gly Ser Glu
85 90 95
Arg Val Ile Asp Glu Ile Arg Glu His Ile Tyr Gln Ile Ser Thr Leu
100 105 110
Ser Asp Phe Gln Tyr Ile Asp Ser Ser Gly Arg Asp Gln Gly Ser Asn
115 120 125
Val Arg Lys Lys Ser Gln Ser Leu Val Ala Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Gln Glu Val Arg Gln Lys Ala Ala Ala Asn Arg Asp Lys Phe
145 150 155 160
Arg Asn Thr Asn Ser Ala Gly Gly Met Tyr Arg Pro Ser Ser Tyr Ser
165 170 175
Ser Ser Gly Gly Tyr Gly Asp Arg Tyr Asp Asp Asp Arg Tyr Glu Gly
180 185 190
Arg Tyr Gly Arg Asp Glu Asp Arg Asn Gly Tyr Gly Arg Glu Arg Glu
195 200 205
Trp Gly Ser Arg Asp Asp Asp Arg Tyr Gly Arg Asn Gly Asp Ser Tyr
210 215 220
Glu Ile Leu Met Asn Gly Met Val Glu Met Ala Ile Arg Met Met Ile
225 230 235 240
Thr Gly Glu Glu Val Glu Ala Met Arg Thr Thr Ser Met Ala Leu Glu
245 250 255
Val Gly Val Leu Ile Glu Ile Gly Thr Glu Ser Asn His Ser Ser Arg
260 265 270
Gly Gly Ala Arg Thr Asp Glu His Pro Gln Tyr Gly Arg Gln Leu Glu
275 280 285
Arg Lys Phe Ser Glu Gln Asn Leu Asp Ala Pro Pro Ser Tyr Glu Glu
290 295 300
Ala Val Ala Asp Ala His Ser Pro Val His Asp Glu Arg Asp Gly Ala
305 310 315 320
Thr Pro Ala Ala Pro Ala Pro Lys Thr Ser Ser Pro Pro Val Ser Thr
325 330 335
Ser Pro Ser Gln Ala Thr Thr Ala Val Gly Pro Ser Thr Ser Pro Pro
340 345 350
Ala Asn Lys Glu Val Asp Ala Phe Asp Glu Phe Asp Pro Arg Gly Pro
355 360 365
Val Ser Gly Met Glu Asn Pro Arg Asp Leu Ser Val Cys Gln Tyr Val
370 375 380
Gln Leu Glu Ser Lys Gln Phe Ala Cys Ala Ser Val Pro Ala Thr Ser
385 390 395 400
Ile Ser Pro Glu Met Asp Leu Leu Gly Ser Leu Ser Glu Ser Phe Ser
405 410 415
Ser Asn Ser Leu Ala Leu Val Pro Ser Gly Pro Ala Thr Thr Thr Ser
420 425 430
Glu Ala Ala Val Leu Gly Asn Ala Gly Ser Ala Pro Ala Ser Ala Ala
435 440 445
Met Pro Ser Gly Ser Ala Ser Phe Glu Asp Pro Phe Gly Asp Ser Pro
450 455 460
Phe Arg Ala Leu Pro Ser Ala Glu Ser Val Pro Ala Glu Pro Gln Asp
465 470 475 480
Ser Ala Ser Thr Thr Ser Phe Gln Thr Met Asn G1n Thr Ser Gly Pro
485 490 495
Pro Phe Pro Val Thr G1n Gly Val Asp Thr Gly Ser Asn Phe Asp Phe
500 505 510
G1y Asp Thr Phe Pro G1y Ile Thr Tyr Thr Pr0 Ser Gly Val Ser Thr
515 520 525
Ala Gln Pro Pro Ser Ala Ser Pro Gln Phe Ser Pro Gln G1u Gln Trp
530 535 540
Ile Pro G1n Gln Asn Asn Asp Ile Leu Ala Asp Ile Leu Pro Pro Ser
545 550 555 560
Gly Ser Ser Ala Pro A1a Ile Ser Gln Ala A1a Phe Pro Ala Pro Thr
565 570 575
G1y Gln Ser Val Gln Pro Asn Pro Asn Val Val Gly Gly Phe Leu Ala
580 585 590
Gln Ser Gly Ser Ala Asp His Ala Ala Ser Gln Phe Ser Pro Gln Ile
595 600 605
Pro Thr Gly Pro Ala Ala Gln Tyr Asn Asn G1y Asn Phe Leu Ser His
610 615 620
Gly Pro Val Thr His Pro Ser Asn Asp Asn Leu Gly G1y Leu Phe Pro
625 630 635 640
G1n Ala Gly Pro Pro G1y Ser Val Met Ser G1n Ser Thr Pro Pro Ala
645 650 655
Ser Thr Gly Ser Leu A1a Ile Val Pro G1n A1a Ser Lys Asp Lys Phe
660 665 670
Glu Thr Lys Ser Thr Val Trp Ala Asp Thr Leu Ser Arg Gly Leu Val
675 680 685
Asn Leu Asn Ile Ser Gly A1a Lys Ile Asn Pro Leu A1a Asp Ile Gly
690 695 700
Ile Asp Phe Asp Ala Ile Asn Arg Lys Glu Lys Arg Met Glu Lys Pro
705 710 715 720
Lys Pro Ala Thr Thr A1a Pro Val Ser Thr Thr Thr Met Gly Lys Ala
725 730 735
Met Gly Ser Gly Ser G1y Met Gly Arg Ala G1y Ala G1y Ala Leu Arg
740 745 750
Pro Pro Pro Asn Thr Met Met Gly Ser G1y Met Gly Met Gly Met G1y
755 760 765
Met Gly Ser Gly Pro Gly Ala Gly Met Ser Met Gly Met Gly Gly Gly
770 775 780
Pro Gly Ser Gly Met Gly Met Gly Met Gly Gly Ala Pro Gly Val Gly
785 790 795 800
Met Gly Met Gly Met Gly Gly Ala Pro Ala Ala Ala Gly Met Gly Met
805 810 815
Gly Met Gly Met Gly Gly Gly Met Gly Met Gly Gly Tyr Gly Gly Met
820 825 830
Asn Gln Pro Met Gly Met Gly Met Asn Val Gly Met Gly Met Asn Met
835 840 845
Gly Met Gly Gln Gly Ala Gln Met Gln Gln Pro Thr Gly Leu Pro Pro
850 855 860
Gly Gly Tyr Asn Pro Met Met Gly Ser Gly Gly Tyr Ala Pro Gln Gln
865 870 875 880
Pro Tyr Gly Gly Gly Tyr Arg
885
<210>91
<211>3075
<212>DNA
<213>拟南芥
<400>91
atgaagaaag catttggtca aactgtcaga gacctcaaga gaggggtcaa caagaaagtg 60
cttaaagtac ctggaataga acagaaggtt ctagatgcta ccagcaatga gtcctggggt 120
ccgcatggat cacttcttgc ggacattgca catgcttcga gaaattacca tgaataccag 180
attactatgg gagtgttatg gaaacgtctt agtgactctg gaaaaaattg gcggcatgtc 240
tataaggcat tgacagtctt ggagtacatg gtaggccatg gatcagaacg tgtcatagaa 300
gaggttaaag aacatgcata tcaaattacg acattatctg gttttcagta tattgattcc 360
agcggtaaag atcaaggtag caatgtcagg aagaaggcgc agagccttgt ggctttggtt 420
aatgacaaag aaagaataac ggaggtcaga gagaaggctg ctgctaacag agataagtat 480
cataactcaa tgcataggcc gtcaggagga tatggggaca aatatgatta cgaaggccgc 540
tatggagaca gagatgaagg gcgaagtagt tatgggaaag agagagaata tggttacagg 600
gatgatgata gaaatagtcg tgatggcgat cgttattcta gagactctga agaccgatat 660
ggaagagatg gtaacacgga tgacgaatac cgtggaagga gcagaagtgt tgataattac 720
aatggatcaa gaggtaggag ctcggacagg gaacggccta ttgaggatga tggccaatcc 780
tcatcgcggg atagtggtgc gccagctgat gatcattctc aagatgggag aggggggcta 840
gagagaaagt tctctgaaca aaatattggt gctgctccac ctagttatga agaagctgtg 900
agtgaatcac ggagccctgt atacagtgaa agggatggtg gagagacccc acaagttgct 960
cctccaggag ctgctgcttc tcctcttgct gaaaacatca gcgtggacaa caaagctgct 1020
gattttgtta atgaatcatc tcctcagcaa gttgaggctt ttgatgaatt tgatccacga 1080
ggttcagttt cagcagcatg tgcacctaca gctggtgctt cggttcctgc acctatccca 1140
ccaacagtgg tttctacgcc tgccccgcct gcctcaatta atgcagaaat ggatttgctt 1200
ggctctcttt cagatgtatt ttcaccaaat cccttggcta ttgtaacatc tgattccact 1260
tctgttgaaa ccaatggaca agcaaacact ggtttagctc catcgttttc tacttctcag 1320
tcatctactc agccttttga tgatccattt ggtgactctc ctttcaaagc catcacttct 1380
gctgacactg agacaagcca acatcagagt tttggagttc cttttcagcc aacaccacca 1440
acttcaaatc ctaacaatga acataacttt ggttttgggg aagcattttc agctgttacc 1500
gattctgaac ctggcgttca aaatatgcaa gctccaccga acttatccgt ctttcctcaa 1560
gagcagtttg atacatcgca gagcgaaatc gatattcttg ccggcattct cccaccatct 1620
ggaccgccag tttctctgtc tccacaacca gattccacta tgccaacatc ccaatttcat 1680
cccaatggta actcctatga aagctatcat catcaagccg cgcctacaga tctaaacatg 1740
caaggacaaa caccgtttgg tcaagcttca caacaattta acatggtttc acattcacaa 1800
aatcatcatg aaggtatgca attcaacaat gggggcttca cgcaacagcc aggctatgca 1860
ggtccagcaa cctctcaacc tccacaatat actcctggtg tatcttcaca cccaccaagt 1920
gagagttttc ctcaccaacc aggttctgcc acctcagcaa gctcgcaaac tccttacgct 1980
actacaccca atgtatcagc tgggcagttt gatggtggga gtttcatgac acagcaacct 2040
tatggtgtga cacaacaagt tcatgttgtc ccttcccata taccacagcg aactcaatct 2100
ggacctgttg cagcgtttgg gaataataac aatatcgttg gagatatgca ccaacctggt 2160
tctaccccct catcaagctc gcaaactcct taccctacta cgcccaatgc accaagtggt 2220
cagtttgatg gtggaaattt tatgacacaa caaccttatg gtgtgatacc gcaagttcat 2280
ggcgtccctt cccatatacc acagcgaact caatctggac ctgttgcagc gcacgggaat 2340
agtaacaatg ttgttgggga tatgttttca ccagctggac taagttcctt ggagacatca 2400
gcatcgcaac catctctcac acctttaaca ggagcaattg aaattgttcc ccaaaatcag 2460
aagaagtttg agccaaaatc aacaatttgg gctgatacat taagtagagg gctagttaac 2520
ttcaacattt ctggaccgaa aacaaatcca ttggcggata taggagttga cttcgaggca 2580
atcaacagga aagagaaacg gctagagaaa ccaactatca cacaacaaca agtaacatca 2640
actatcaaca tggggaaagc catgggatct ggtactggct taggtcgcgc tggtgcaggt 2700
gctatgagac ctccaacaaa ttcaatggta ggctcaagca tgcccacggg catgaatgtt 2760
ggtggctatg gaggcatgaa ccaacaccaa cccataggca tgaaccaaaa ccatcccatg 2820
ggcatgaacc aaaacctttc catgggaatg aaccagaact atcccatggg aatgaaccaa 2880
aactatccaa tgggaatggg catgaacatg aacatgggcg ggtatggaca agggtatccg 2940
atgcaaccac aacaaggaat gggcatggct cctcctggtg caccacaagg catgaccggt 3000
gcctataatc caatgatggg tcaaggcggt tacaaccctc agcagcagca accatatggt 3060
ggaggttacc ggtaa 3075
<210>92
<211>1024
<212>PRT
<213>拟南芥
<400>92
Met Lys Lys Ala Phe Gly Gln Thr Val Arg Asp Leu Lys Arg Gly Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Ile Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Ser Trp Gly Pro His Gly Ser Leu Leu Ala Asp
35 40 45
Ile Ala His Ala Ser Arg Asn Tyr His Glu Tyr Gln Ile Thr Met Gly
50 55 60
Val Leu Trp Lys Arg Leu Ser Asp Ser Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Met Val Gly His Gly Ser Glu
85 90 95
Arg Val Ile Glu Glu Val Lys Glu His Ala Tyr Gln Ile Thr Thr Leu
100 105 110
Ser Gly Phe Gln Tyr Ile Asp Ser Ser Gly Lys Asp Gln Gly Ser Asn
115 120 125
Val Arg Lys Lys Ala Gln Ser Leu Val Ala Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Thr Glu Val Arg Glu Lys Ala Ala Ala Asn Arg Asp Lys Tyr
145 150 155 160
His Asn Ser Met His Arg Pro Ser Gly Gly Tyr Gly Asp Lys Tyr Asp
165 170 175
Tyr Glu Gly Arg Tyr Gly Asp Arg Asp Glu Gly Arg Ser Ser Tyr Gly
180 185 190
Lys Glu Arg Glu Tyr Gly Tyr Arg Asp Asp Asp Arg Asn Ser Arg Asp
195 200 205
Gly Asp Arg Tyr Ser Arg Asp Ser Glu Asp Arg Tyr Gly Arg Asp Gly
210 215 220
Asn Thr Asp Asp Glu Tyr Arg Gly Arg Ser Arg Ser Val Asp Asn Tyr
225 230 235 240
Asn Gly Ser Arg Gly Arg Ser Ser Asp Arg Glu Arg Pro Ile Glu Asp
245 250 255
Asp Gly Gln Ser Ser Ser Arg Asp Ser Gly Ala Pro Ala Asp Asp His
260 265 270
Ser Gln Asp Gly Arg Gly Gly Leu Glu Arg Lys Phe Ser Glu Gln Asn
275 280 285
Ile Gly Ala Ala Pro Pro Ser Tyr Glu Glu Ala Val Ser Glu Ser Arg
290 295 300
Ser Pro Val Tyr Ser Glu Arg Asp Gly Gly Glu Thr Pro Gln Val Ala
305 310 315 320
Pro Pro Gly Ala Ala Ala Ser Pro Leu Ala Glu Asn Ile Ser Val Asp
325 330 335
Asn Lys Ala Ala Asp Phe Val Asn Glu Ser Ser Pro Gln Gln Val Glu
340 345 350
Ala Phe Asp Glu Phe Asp Pro Arg Gly Ser Val Ser Ala Ala Cys Ala
355 360 365
Pro Thr Ala Gly Ala Ser Val Pro Ala Pro Ile Pro Pro Thr Val Val
370 375 380
Ser Thr Pro Ala Pro Pro Ala Ser Ile Asn Ala Glu Met Asp Leu Leu
385 390 395 400
Gly Ser Leu Ser Asp Val Phe Ser Pro Asn Pro Leu Ala Ile Val Thr
405 410 415
Ser Asp Ser Thr Ser Val Glu Thr Asn Gly Gln Ala Asn Thr Gly Leu
420 425 430
Ala Pro Ser Phe Ser Thr Ser Gln Ser Ser Thr Gln Pro Phe Asp Asp
435 440 445
Pro Phe Gly Asp Ser Pro Phe Lys Ala Ile Thr Ser Ala Asp Thr Glu
450 455 460
Thr Ser Gln His Gln Ser Phe Gly Val Pro Phe Gln Pro Thr Pro Pro
465 470 475 480
Thr Ser Asn Pro Asn Asn Glu His Asn Phe Gly Phe Gly Glu Ala Phe
485 490 495
Ser Ala Val Thr Asp Ser Glu Pro Gly Val Gln Asn Met Gln Ala Pro
500 505 510
Pro Asn Leu Ser Val Phe Pro Gln Glu Gln Phe Asp Thr Ser Gln Ser
515 520 525
Glu Ile Asp Ile Leu Ala Gly Ile Leu Pro Pro Ser Gly Pro Pro Val
530 535 540
Ser Leu Ser Pro Gln Pro Asp Ser Thr Met Pro Thr Ser Gln Phe His
545 550 555 560
Pro Asn Gly Asn Ser Tyr Glu Ser Tyr His His Gln Ala Ala Pro Thr
565 570 575
Asp Leu Asn Met Gln Gly Gln Thr Pro Phe Gly Gln Ala Ser Gln Gln
580 585 590
Phe Asn Met Val Ser His Ser Gln Asn His His Glu Gly Met Gln Phe
595 600 605
Asn Asn Gly Gly Phe Thr Gln Gln Pro Gly Tyr Ala Gly Pro Ala Thr
610 615 620
Ser Gln Pro Pro Gln Tyr Thr Pro Gly Val Ser Ser His Pro Pro Ser
625 630 635 640
Glu Ser Phe Pro His Gln Pro Gly Ser Ala Thr Ser Ala Ser Ser Gln
645 65 0655
Thr Pro Tyr Ala Thr Thr Pro Asn Val Ser Ala Gly Gln Phe Asp Gly
660 665 670
Gly Ser Phe Met Thr Gln Gln Pro Tyr Gly Val Thr Gln Gln Val His
675 680 685
Val Val Pro Ser His Ile Pro Gln Arg Thr Gln Ser Gly Pro Val Ala
690 695 700
Ala Phe Gly Asn Asn Asn Asn Ile Val Gly Asp Met His Gln Pro Gly
705 710 715 720
Ser Thr Pro Ser Ser Ser Ser Gln Thr Pro Tyr Pro Thr Thr Pro Asn
725 730 735
Ala Pro Ser Gly Gln Phe Asp Gly Gly Asn Phe Met Thr Gln Gln Pro
740 745 750
Tyr Gly Val Ile Pro Gln Val His Gly Val Pro Ser His Ile Pro Gln
755 760 765
Arg Thr Gln Ser Gly Pro Val Ala Ala His Gly Asn Ser Asn Asn Val
770 775 780
Val Gly Asp Met Phe Ser Pro Ala Gly Leu Ser Ser Leu Glu Thr Ser
785 790 795 800
Ala Ser Gln Pro Ser Leu Thr Pro Leu Thr Gly Ala Ile Glu Ile Val
805 810 815
Pro Gln Asn Gln Lys Lys Phe Glu Pro Lys Ser Thr Ile Trp Ala Asp
820 825 830
Thr Leu Ser Arg Gly Leu Val Asn Phe Asn Ile Ser Gly Pro Lys Thr
835 840 845
Asn Pro Leu Ala Asp Ile Gly Val Asp Phe Glu Ala Ile Asn Arg Lys
850 855 860
Glu Lys Arg Leu Glu Lys Pro Thr Ile Thr Gln Gln Gln Val Thr Ser
865 870 875 880
Thr Ile Asn Met Gly Lys Ala Met Gly Ser Gly Thr Gly Leu Gly Arg
885 890 895
Ala Gly Ala Gly Ala Met Arg Pro Pro Thr Asn Ser Met Val Gly Ser
900 905 910
Ser Met Pro Thr Gly Met Asn Val Gly Gly Tyr Gly Gly Met Asn Gln
915 920 925
His Gln Pro Ile Gly Met Asn Gln Asn His Pro Met Gly Met Asn Gln
930 935 940
Asn Leu Ser Met Gly Met Asn Gln Asn Tyr Pro Met Gly Met Asn Gln
945 950 955 960
Asn Tyr Pro Met Gly Met Gly Met Asn Met Asn Met Gly Gly Tyr Gly
965 970 975
Gln Gly Tyr Pro Met Gln Pro Gln Gln Gly Met Gly Met Ala Pro Pro
980 985 990
Gly Ala Pro Gln Gly Met Thr Gly Ala Tyr Asn Pro Met Met Gly Gln
995 1000 1005
Gly Gly Tyr Asn Pro Gln Gln Gln Gln Pro Tyr Gly Gly Gly Tyr
1010 1015 1020
Arg
<210>93
<211>804
<212>DNA
<213>拟南芥
<400>93
atgaagaaag tcttcggaca aactgttaga gaccttaaga gagaggtcaa caagaaagtg 60
ctcaaagttc ctggagtaga acagaaggtt ctagatgcta ctagtaatga gccatggggt 120
ccacatggat cacttcttgc tgatcttgcg caagcttcca gaaattatca tgaatatcag 180
ctgatcatgg tggtcatatg gaaacgcctt agcgacaccg gaaaaaactg gcggcatgtc 240
tataaggctt tgacagtttt ggagtacatg gtaggccatg gatcagaacg tgttatagac 300
gagattagag aacgtgcata tcaaatttcg acattgtccg attttcagta tattgattcc 360
ggtggtagag atcaaggaag caatgttagg aagaaatcac agagcctggt ggctttggtg 420
aatgacaagg aaagaatagc tgaagtcaga cagaaggctg ctgctaatag agataagtat 480
cgcagctcag caccaggtgg gatgtataag ccttcaggcg gatatgggga taaatatgat 540
tacggaagcc gggatgaaga gcgaagtagt tatggaagag aaagagaata tggttacaga 600
gatgatgata gaaatagtcg tgatggagat cgtcattcca gggactctga agaccggtat 660
gggagagatg gtaataggga tgatgattac aggggaagga gcagaagtgt tgataactat 720
gggtcacgag gtaggagttc ggaaagagag cgggaggatg atggccattc ttcatcacgg 780
tatgttaaat ttcaagtcgc ctaa 804
<210>94
<211>267
<212>PRT
<213>拟南芥
<400>94
Met Lys Lys Val Phe Gly Gln Thr Val Arg Asp Leu Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Val Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser Leu Leu Ala Asp
35 40 45
Leu Ala Gln Ala Ser Arg Asn Tyr His Glu Tyr Gln Leu Ile Met Val
50 55 60
Val Ile Trp Lys Arg Leu Ser Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Met Val Gly His Gly Ser Glu
85 90 95
Arg Val Ile Asp Glu Ile Arg Glu Arg Ala Tyr Gln Ile Ser Thr Leu
100 105 110
Ser Asp Phe Gln Tyr Ile Asp Ser Gly Gly Arg Asp Gln Gly Ser Asn
115 120 125
Val Arg Lys Lys Ser Gln Ser Leu Val Ala Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Ala Glu Val Arg Gln Lys Ala Ala Ala Asn Arg Asp Lys Tyr
145 150 155 160
Arg Ser Ser Ala Pro Gly Gly Met Tyr Lys Pro Ser Gly Gly Tyr Gly
165 170 175
Asp Lys Tyr Asp Tyr Gly Ser Arg Asp Glu Glu Arg Ser Ser Tyr Gly
180 185 190
Arg Glu Arg Glu Tyr Gly Tyr Arg Asp Asp Asp Arg Asn Ser Arg Asp
195 200 205
Gly Asp Arg His Ser Arg Asp Ser Glu Asp Arg Tyr Gly Arg Asp Gly
210 215 220
Asn Arg Asp Asp Asp Tyr Arg Gly Arg Ser Arg Ser Val Asp Asn Tyr
225 230 235 240
Gly Ser Arg Gly Arg Ser Ser Glu Arg Glu Arg Glu Asp Asp Gly His
245 250 255
Ser Ser Ser Arg Tyr Val Lys Phe Gln Val Ala
260 265
<210>95
<211>2454
<212>DNA
<213>葡萄
<400>95
atgcctggaa gtgatgagcg aatggttctg gatgctacta gcaatgagcc ttggggtcct 60
catggaacac atcttgcaga tattgcacag gctaccagaa actatcacga gtaccaaatg 120
atcatgtcag taatctggaa gcggattaat gatacaggaa agaattggcg gcatgtctac 180
aagactttgt cagactttca atatattgat tcaagtggga gggatcaggg aagcaatgtc 240
agaaagaaat ctcaaagtct cgtggctctg gtgaatgata aagaaaggat acaggaagtt 300
agacagaagg ctgctgccaa cagggacaag tttcgcaaca caaactcagc aggcggtatg 360
tataggccca gttcatattc tagttcagga ggatatggtg accgttatga tgatgatcga 420
tatgaaggcc gctatggaag agacgaagat cgaaatggtt atgggagaga aagagaatgg 480
ggcagcagag atgatgaccg gtatggtaga aatggggact catatggtcc tgaaggggat 540
cgctatggca gagattctga tgaacggtat ggtagagatg gttataagga tgatgattac 600
aggggaagaa gtcgaagcaa tgaggactac cagtatggct ctagaagtag gagtgctgat 660
agagataggg accgtgcttt tgatgaggag agcaaccatt catctagagg tggtgcccga 720
actgatgagc atcctcagta tggaaggcag cttgaacgaa aattttctga acaaaatctt 780
gatgctcctc ctagttatga agaagctgta gctgatgccc acagtcctgt tcatgatgag 840
agggatggag caaccccagc agcacctgcc cctaaaacat cttctccacc tgtaagcact 900
agtcctagcc aagcaacaac tgctgttggt ccttctacat ctcctcctgc caacaaggaa 960
gttgatgctt ttgatgaatt tgatccgcgc ggtccagttt cagctgtccc agctacatca 1020
attagtccgg aaatggactt acttggctct ctgtcagagt cattttcttc aaattcattg 1080
gctcttgtgc catctggacc tgcaaccaca acatctgagg ccgctgtcct tggaaatgct 1140
gggtctgcac ccgcatctgc agcaaagcca tctggatctg ctgttatgag ccagtctttt 1200
gaagacccat ttggtgactc cccttttaga gctttgcctt cagcagagag tgtaccagct 1260
caacctcagg attcagcttc cacaacttcc ttccagacca tgaatcaaac atcaggacca 1320
cccttcccag ttacccaagg agtggacacg ggttccaact ttgactttgg ggacactttc 1380
cctggcataa cttacacgcc atccggtgtc tctactgcac aacctccttc tgcaagccca 1440
caattttcac cccaagagca atggtttccc cagcagaaca atgatatcct agcagatatt 1500
ctaccacctt caggatcttc agctcctgcc atttcacagg ctgctttccc agctccaact 1560
ggccagtctg tgcagccaaa ccctaatgtt gttgggggct ttcttgcaca gtcaggatct 1620
gcagatcatg cagcttcaca attttctcct cagattccaa ctggaccagc tgcacaatat 1680
aacaatggga acttcctttc acagttgggt tctgcagcgc ctgtaccttc acaggctcct 1740
cttcaargtc ctgtaccttc tcagtctcct tttcaaagtc ctgtaccttc tcagtctcct 1800
tttcaagctc ctgtaccttc tcagtctcct tttcaagctc cagctaaaat agtgtcttca 1860
tatgcagcta aaattaatcc attggcggat attggaattg attttgatgc cattaatcgg 1920
aaagaaaaga ggatggagaa acctaagcct gctaccacag ctcctgtatc tactactaca 1980
atgggcaagg caatgggatc tggttctgga atgggtcgtg ctggtgcagg tgcactcagg 2040
cctccaccaa atactatgat gggttcaggt atgggaatgg gcatgggcat gggcatgggt 2100
agtggccctg gtgcaggcat gagtatgggt atgggtggtg gtcctggttc aggcatgggt 2160
atgggtatgg gtggtgcccc tggagtgggc atgggtatgg gtatgggtgg tgcccctgca 2220
gcagcgggta tgggtatggg tatgggtatg gggatgggag gaggcatggg catgggagga 2280
tatggaggca tgaatcaacc tatgggcatg ggtatgaatg tgggcatgaa tatgggaatg 2340
gggcaaggag cccagatgca acaaccaact ggattgcctc ctgggggtta taaccccatg 2400
atgggctcag gtggttatgc tcctcagcaa ccatatggtg gtggctaccg atga 2454
<210>96
<211>817
<212>PRT
<213>葡萄
<220>
<221>不确定
<222>(583)..(583)
<223>Xaa可以是任意天然氨基酸
<400>96
Met Pro Gly Ser Asp Glu Arg Met Val Leu Asp Ala Thr Ser Asn Glu
1 5 10 15
Pro Trp Gly Pro His Gly Thr His Leu Ala Asp Ile Ala Gln Ala Thr
20 25 30
Arg Asn Tyr His Glu Tyr Gln Met Ile Met Ser Val Ile Trp Lys Arg
35 40 45
Ile Asn Asp Thr Gly Lys Asn Trp Arg His Val Tyr Lys Thr Leu Ser
50 55 60
Asp Phe Gln Tyr Ile Asp Ser Ser Gly Arg Asp Gln Gly Ser Asn Val
65 70 75 80
Arg Lys Lys Ser Gln Ser Leu Val Ala Leu Val Asn Asp Lys Glu Arg
85 90 95
Ile Gln Glu Val Arg Gln Lys Ala Ala Ala Asn Arg Asp Lys Phe Arg
100 105 110
Asn Thr Asn Ser Ala Gly Gly Met Tyr Arg Pro Ser Ser Tyr Ser Ser
115 120 125
Ser Gly Gly Tyr Gly Asp Arg Tyr Asp Asp Asp Arg Tyr Glu Gly Arg
130 135 140
Tyr Gly Arg Asp Glu Asp Arg Asn Gly Tyr Gly Arg Glu Arg Glu Trp
145 150 155 160
Gly Ser Arg Asp Asp Asp Arg Tyr Gly Arg Asn Gly Asp Ser Tyr Gly
165 170 175
Pro Glu Gly Asp Arg Tyr Gly Arg Asp Ser Asp Glu Arg Tyr Gly Arg
180 185 190
Asp Gly Tyr Lys Asp Asp Asp Tyr Arg Gly Arg Ser Arg Ser Asn Glu
195 200 205
Asp Tyr Gln Tyr Gly Ser Arg Ser Arg Ser Ala Asp Arg Asp Arg Asp
210 215 220
Arg Ala Phe Asp Glu Glu Ser Asn His Ser Ser Arg Gly Gly Ala Arg
225 230 235 240
Thr Asp Glu His Pro Gln Tyr Gly Arg Gln Leu Glu Arg Lys Phe Ser
245 250 255
Glu Gln Asn Leu Asp Ala Pro Pro Ser Tyr Glu Glu Ala Val Ala Asp
260 265 270
Ala His Ser Pro Val His Asp Glu Arg Asp Gly Ala Thr Pro Ala Ala
275 280 285
Pro Ala Pro Lys Thr Ser Ser Pro Pro Val Ser Thr Ser Pro Ser Gln
290 295 300
Ala Thr Thr Ala Val Gly Pro Ser Thr Ser Pro Pro Ala Asn Lys Glu
305 310 315 320
Val Asp Ala Phe Asp Glu Phe Asp Pro Arg Gly Pro Val Ser Ala Val
325 330 335
Pro Ala Thr Ser Ile Ser Pro Glu Met Asp Leu Leu Gly Ser Leu Ser
340 345 350
Glu Ser Phe Ser Ser Asn Ser Leu Ala Leu Val Pro Ser Gly Pro Ala
355 360 365
Thr Thr Thr Ser Glu Ala Ala Val Leu Gly Asn Ala Gly Ser Ala Pro
370 375 380
Ala Ser Ala Ala Lys Pro Ser Gly Ser Ala Val Met Ser Gln Ser Phe
385 390 395 400
Glu Asp Pro Phe Gly Asp Ser Pro Phe Arg Ala Leu Pro Ser Ala Glu
405 410 415
Ser Val Pro Ala Gln Pro Gln Asp Ser Ala Ser Thr Thr Ser Phe Gln
420 425 430
Thr Met Asn Gln Thr Ser Gly Pro Pro Phe Pro Val Thr Gln Gly Val
435 440 445
Asp Thr Gly Ser Asn Phe Asp Phe Gly Asp Thr Phe Pro Gly Ile Thr
450 455 460
Tyr Thr Pro Ser Gly Val Ser Thr Ala Gln Pro Pro Ser Ala Ser Pro
465 470 475 480
Gln Phe Ser Pro Gln Glu Gln Trp Phe Pro Gln Gln Asn Asn Asp Ile
485 490 495
Leu Ala Asp Ile Leu Pro Pro Ser Gly Ser Ser Ala Pro Ala Ile Ser
500 505 510
Gln Ala Ala Phe Pro Ala Pro Thr Gly Gln Ser Val Gln Pro Asn Pro
515 520 525
Asn Val Val Gly Gly Phe Leu Ala Gln Ser Gly Ser Ala Asp His Ala
530 535 540
Ala Ser Gln Phe Ser Pro Gln Ile Pro Thr Gly Pro Ala Ala Gln Tyr
545 550 555 560
Asn Asn Gly Asn Phe Leu Ser Gln Leu Gly Ser Ala Ala Pro Val Pro
565 570 575
Ser Gln Ala Pro Leu Gln Xaa Pro Val Pro Ser Gln Ser Pro Phe Gln
580 585 590
Ser Pro Val Pro Ser Gln Ser Pro Phe Gln Ala Pro Val Pro Ser Gln
595 600 605
Ser Pro Phe Gln Ala Pro Ala Lys Ile Val Ser Ser Tyr Ala Ala Lys
610 615 620
Ile Asn Pro Leu Ala Asp Ile Gly Ile Asp Phe Asp Ala Ile Asn Arg
625 630 635 640
Lys Glu Lys Arg Met Glu Lys Pro Lys Pro Ala Thr Thr Ala Pro Val
645 650 655
Ser Thr Thr Thr Met Gly Lys Ala Met Gly Ser Gly Ser Gly Met Gly
660 665 670
Arg Ala Gly Ala Gly Ala Leu Arg Pro Pro Pro Asn Thr Met Met Gly
675 680 685
Ser Gly Met Gly Met Gly Met Gly Met Gly Met Gly Ser Gly Pro Gly
690 695 700
Ala Gly Met Ser Met Gly Met Gly Gly Gly Pro Gly Ser Gly Met Gly
705 710 715 720
Met Gly Met Gly Gly Ala Pro Gly Val Gly Met Gly Met Gly Met Gly
725 730 735
Gly Ala Pro Ala Ala Ala Gly Met Gly Met Gly Met Gly Met Gly Met
740 745 750
Gly Gly Gly Met Gly Met Gly Gly Tyr Gly Gly Met Asn Gln Pro Met
755 760 765
Gly Met Gly Met Asn Val Gly Met Asn Met Gly Met Gly Gln Gly Ala
770 775 780
Gln Met Gln Gln Pro Thr Gly Leu Pro Pro Gly Gly Tyr Asn Pro Met
785 790 795 800
Met Gly Ser Gly Gly Tyr Ala Pro Gln Gln Pro Tyr Gly Gly Gly Tyr
805 810 815
Arg
<210>97
<211>1632
<212>DNA
<213>莱茵衣藻(chlamydomonas reinhardtii)
<400>97
atgtggcaca ctgtggcctt cacgaagctc aaagtgctgg aggcgaccaa cgaggagcca 60
tggggacccc acgggtccgc gatgggggag attgcgcgtg cagcggagga cccagagaaa 120
tacaacctca tcatgaacgt catcagcgag cgcctgcaga tgcgggacga gaactggcgc 180
ctgtgctaca aggcgctgct gctgctcgag taccttgtta aaaacgggcc ttggcgtgtg 240
gtagacgagc tcaaccgcag cgtgtcctcg ctggagcgac tgcgtgatga gtttgagtac 300
cgtgacccgc agggcaagga ccacggtgtc aacgtgcggc agagggcggg tgagctggcc 360
tcactcgtca gcaacaccga ccgcgttcgc caggagcgtg aaaaggctgc caaaaacgcc 420
aacaagtata agggcgtatc gtcgtcggac atgcgcggat ttggtggcgg taacctgaca 480
tggcttcctg cgcgccccca gcaggcctac cccggcacgg agagcgtgca catgcctgcc 540
ggcagcagca gcagccacca cagccgcacc ggatcgggct ccaacgccaa cacctttaac 600
gccttcaaca accccacagc cgccggccac tcgggcggcg gctttggtgg gctgcgcggc 660
actggaccgg cggctgggtc tggggtgggg cccgaggagg gcgaggaccc attcgaggcc 720
acccgcaagc ggatagagcg gctgaaggcg gagggcgcgc tgccggagcc gccgccgtca 780
gcgctgccgc cgggtctggc agacgtgccg gccgcaggcg ccaaggctcc caagaagctg 840
tccgaaatca aaatcaaccc cgcggtggcc gccacgttcg cgtccatgcc catcgcgccg 900
ccgcccagcg gtgcaaccat aggcaagctg gcaccgcctc cgggcgccgg tggtgctgcc 960
aagccgccgc tgccgctgcc cgccgcgagc aacaccctgg acctgtttgg ggagctgagc 1020
ggccccacat cctcgcagcc cgctcagtca cagcccgcgg ccagcgctgc ttcggactgg 1080
gacgctttcg gctcggcgcc agctgttgcg ccggcgcccg ctgcggctcc ggccgcgccg 1140
gtggatccct ttgccgtgct cgcctctgga cccaccgcgg ccacagcaac atctggtggc 1200
agcgcctcgt tgggcggctt taagcccgcg gtagcgacgc acgcgagtac ctcttccaag 1260
ccgggcgtgc cgccgccgct gtcattcgac gacttcgctg agctgcccgc cgcgccgcct 1320
gcagcggggg cagacccctt cgcggcactg gcaagcggcg gctccgcggc ggcttcgcgc 1380
ccagcggtac cagcgccagc acctgcggcg gccttcgacc cgtttgcgcc ggcgccgtcg 1440
accatggcga cagcgcctgc gacgagcagt gggtttggcg actttatgag cggcaaccgg 1500
tcctcatcgc acgccagctc cggcggcgcg gctagctcgg gcggcgcgac catggcaatg 1560
aacaaggcgg gcgcggcgca cggggctgcg gggcaaaaga gcaacgaccc gtttgcaggg 1620
ctagggttct ag 1632
<210>98
<211>543
<212>PRT
<213>莱茵衣藻
<400>98
Met Trp His Thr Val Ala Phe Thr Lys Leu Lys Val Leu Glu Ala Thr
1 5 10 15
Asn Glu Glu Pro Trp Gly Pro His Gly Ser Ala Met Gly Glu Ile Ala
20 25 30
Arg Ala Ala Glu Asp Pro Glu Lys Tyr Asn Leu Ile Met Asn Val Ile
35 40 45
Ser Glu Arg Leu Gln Met Arg Asp Glu Asn Trp Arg Leu Cys Tyr Lys
50 55 60
Ala Leu Leu Leu Leu Glu Tyr Leu Val Lys Asn Gly Pro Trp Arg Val
65 70 75 80
Val Asp Glu Leu Asn Arg Ser Val Ser Ser Leu Glu Arg Leu Arg Asp
85 90 95
Glu Phe Glu Tyr Arg Asp Pro Gln Gly Lys Asp His Gly Val Asn Val
100 105 110
Arg Gln Arg Ala Gly Glu Leu Ala Ser Leu Val Ser Asn Thr Asp Arg
115 120 125
Val Arg G1n Glu Arg Glu Lys Ala A1a Lys Asn Ala Asn Lys Tyr Lys
130 135 140
Gly Val Ser Ser Ser Asp Met Arg Gly Phe Gly Gly Gly Asn Leu Thr
145 150 155 160
Trp Leu Pro Ala Arg Pro Gln G1n Ala Tyr Pro Gly Thr Glu Ser Val
165 170 175
His Met Pro Ala G1y Ser Ser Ser Ser His His Ser Arg Thr G1y Ser
180 185 190
Gly Ser Asn Ala Asn Thr Phe Asn Ala Phe Asn Asn Pro Thr Ala Ala
195 200 205
G1y His Ser Gly Gly Gly Phe Gly Gly Leu Arg Gly Thr Gly Pro Ala
210 215 220
Ala Gly Ser Gly Val Gly Pro Glu Glu Gly Glu Asp Pro Phe Glu Ala
225 230 235 240
Thr Arg Lys Arg Ile G1u Arg Leu Lys Ala Glu G1y Ala Leu Pro Glu
245 250 255
Pro Pro Pro Ser A1a Leu Pro Pro Gly Leu A1a Asp Val Pro Ala Ala
260 265 270
Gly Ala Lys Ala Pro Lys Lys Leu Ser Glu Ile Lys Ile Asn Pro Ala
275 280 285
Val Ala Ala Thr Phe Ala Ser Met Pro Ile Ala Pro Pro Pro Ser Gly
290 295 300
Ala Thr Ile Gly Lys Leu Ala Pro Pro Pro Gly Ala Gly Gly Ala Ala
305 310 315 320
Lys Pro Pro Leu Pro Leu Pro Ala Ala Ser Asn Thr Leu Asp Leu Phe
325 330 335
G1y Glu Leu Ser G1y Pro Thr Ser Ser Gln Pro Ala Gln Ser G1n Pro
340 345 350
Ala Ala Ser A1a Ala Ser Asp Trp Asp A1a Phe Gly Ser A1a Pro Ala
355 360 365
Val Ala Pro Ala Pro Ala Ala Ala Pro Ala Ala Pro Val Asp Pro Phe
370 375 380
A1a Val Leu A1a Ser Gly Pro Thr Ala Ala Thr Ala Thr Ser Gly Gly
385 390 395 400
Ser Ala Ser Leu Gly Gly Phe Lys Pro A1a Val Ala Thr His Ala Ser
405 410 415
Thr Ser Ser Lys Pro G1y Val Pro Pro Pro Leu Ser Phe Asp Asp Phe
420 425 430
A1a Glu Leu Pro Ala Ala Pro Pro Ala Ala Gly Ala Asp Pro Phe Ala
435 440 445
A1a Leu Ala Ser G1y Gly Ser Ala Ala Ala Ser Arg Pro Ala Val Pro
450 455 460
A1a Pro Ala Pro Ala Ala Ala Phe Asp Pro Phe Ala Pro Ala Pro Ser
465 470 475 480
Thr Met Ala Thr Ala Pro Ala Thr Ser Ser G1y Phe G1y Asp Phe Met
485 490 495
Ser G1y Asn Arg Ser Ser Ser His Ala Ser Ser G1y Gly Ala Ala Ser
500 505 510
Ser Gly Gly A1a Thr Met Ala Met Asn Lys Ala Gly Ala Ala His Gly
515 520 525
A1a Ala Gly Gln Lys Ser Asn Asp Pro Phe Ala Gly Leu G1y Phe
530 535 540
<210>99
<211>594
<212>DNA
<213>Ostreococcus lucimarinus
<400>99
atgttcaaag ccgcgctgac gacgatcaag tccgcgatcc cggacgaggt ggcgcaggcg 60
gcgaagaagc gcgtcaatca atggaaaggc atcggcgacg acgaagcgct cgtccgcgac 120
gcgacgaact ccgagccctg gggcccgcac ggggagcagc tgcgcgcgat cgcgcggctc 180
acgcgcgatg ggaagtggga cgtcgtgcgc gaggtgctgg agaaacgctt gaagagcgcg 240
ccggaggagt ggcgacgcgc gtacaaggcg ctcaccgtcg tcgagtatct cgtcgcgaac 300
ggcgatcgag cgatcgcgga ggacgtcagg cgaaggcgaa tgatggacgg cgcgctgagg 360
ttcgagtaca aggacgcgcg agggaaggat gagggggtga acgtgcgaca tcgagcggaa 420
aagatcaagg cgttggtgga agatccgaga tcggtcgagg aggcgaggga aaaggcggag 480
agaaatcgcg gcaagtacgc ggggatgtcg agcgaagaag cgcggacgca cgcgcggaga 540
gggtcgacgt cgagcgcggg tgggtcgttt tcgggaggga gcgcgctcgg cggc 594
<210>100
<211>198
<212>PRT
<213>Ostreococcus lucimarinus
<400>100
Met Phe Lys Ala Ala Leu Thr Thr Ile Lys Ser Ala Ile Pro Asp G1u
1 5 10 15
Val Ala Gln Ala Ala Lys Lys Arg Val Asn Gln Trp Lys Gly Ile Gly
20 25 30
Asp Asp Glu Ala Leu Val Arg Asp Ala Thr Asn Ser Glu Pro Trp Gly
35 40 45
Pro His Gly Glu Gln Leu Arg Ala Ile Ala Arg Leu Thr Arg Asp Gly
50 55 60
Lys Trp Asp Val Val Arg Glu Val Leu Glu Lys Arg Leu Lys Ser Ala
65 70 75 80
Pro Glu Glu Trp Arg Arg Ala Tyr Lys Ala Leu Thr Val Val Glu Tyr
85 90 95
Leu Val Ala Asn Gly Asp Arg Ala Ile Ala Glu Asp Val Arg Arg Arg
100 105 110
Arg Met Met Asp Gly Ala Leu Arg Phe Glu Tyr Lys Asp Ala Arg Gly
115 120 125
Lys Asp Glu Gly Val Asn Val Arg His Arg Ala Glu Lys Ile Lys Ala
130 135 140
Leu Val Glu Asp Pro Arg Ser Val Glu Glu Ala Arg Glu Lys Ala Glu
145 150 155 160
Arg Asn Arg Gly Lys Tyr Ala Gly Met Ser Ser Glu Glu Ala Arg Thr
165 170 175
His Ala Arg Arg Gly Ser Thr Ser Ser Ala Gly Gly Ser Phe Ser Gly
180 185 190
Gly Ser Ala Leu Gly Gly
195
<210>101
<211>2435
<212>DNA
<213>稻
<400>101
ggtcgttatt ccgcggcgcc atcttcctct ctcctccgcc ctacgcgtcg ccccgcctcg 60
cctggccttc cccgcgcatc ctgcacggag ccctaggagg ctaggagagg aagaagaaca 120
cgcgcgcggg gggatagtgg atagccgaag caactcgatc gatctgtggc cgcgatcgta 180
gctctcttct tggtccgatc tcgtctctcc cggcatggat ttcaggaagg tgctcgacca 240
gaccgtccgg gagataagga gggaggtaaa tcttaaggtg ctcaaggtgc cggaaatcga 300
gcagaaggtt cttgatgcca ccagcgacga gccgtggggg ccgcatggtt ccgatttggc 360
agatatcgcc agggccacca agagctacgg tgatagcgaa attataatga atgtgttatg 420
gcaacgcctg gggaacacac ttgcgaattg gcgtcacgtg tataaggcgt tggctgtgat 480
cgagtacctt ctagctaatg gcaccgaacg tgcagctgat ggcattgttg acaatagctc 540
acgaattgca aaactcacaa gatttgagta tttggagcct aatggaaaag atgttgggct 600
caatgtgcgt aagaaggctg aagctgttct agcaattttg gatgacaggg agaagcttca 660
agaggtcaga gagaaggctg ccgttactag agacaagtat tttggcttat catcaactgg 720
aataacgcac aaatcgagcg cagcatcatt tggcagtggc agctactcat ctggtagcca 780
ctatgggagc acaggaggtt caagggaggt gggatcattc aaggatatac acacaggcac 840
agaatggaaa aagaacaaga aggaaacagt gtcaaactac agcagcaata gagaagggtc 900
taaagaaatt actaacagtg caaccagtta taagtcaaaa aagagtgaaa ggcatggtag 960
aagaaatcaa aattccttaa cattacactc gaagttatct gcaaatatta gcaccacatc 1020
tgaagcccca agctcaaaga aaggggaaaa tgaggatgat gatgatttca acccacgagg 1080
attttctaca tctactggaa caggtaccac aagatctaat cacctggatc tctttggtcc 1140
aagcttgatg gatgatcttg ttgattctac tacatccact tcaacagcaa cgccaaatgt 1200
tagcacacct gctgtgccag aggttgattt atttgcagat gcagctttcc aatcagccaa 1260
tgctccattg gaggcagcaa cggtttctca cactcaggac aaaattgatt tgtttgctgg 1320
cagactgtct tctgctgatt catttacttc agacacagag ttctcagtac gcggtagtcc 1380
taacaagtca tcggagaaaa aaatgtcttc cgttgtgcat ccttctactt ctgcttttga 1440
tcccttcaaa caatcttttg ccacctcatt tccttcagat tcagagttct cagttcatga 1500
tccgacaagc aaatcttctc aaggaaaaac tcccacacca gaacattcaa gtacagcagc 1560
ttttgatcct tttgctgcaa ttccactgaa gagttttgat ggatccgaat cttttggaac 1620
attttcttcg aacacagcct caaacattac tgaactgcca cgggattctt ctgggggtcc 1680
caaaagttct gaccatggtc ctttggagga tgccaatttt gatgccttca cttcacactt 1740
gggatcttcc acaacaagtg caactgagtc catgaataag cccatcaaaa agcttgggca 1800
ggactcgatg tcagcatcaa aatcagttgc aaagaaagaa acttttcagg tcaaatctgg 1860
catatgggct gactctttga gccgaggatt gattgatttg aatataactt cgtcacaaaa 1920
gaaggtcgat ctctccgatg ttgggattgt cggaccgctg agcggcggat ctgaggataa 1980
aggcccgggg gcgacgatgg gcacagcacc aggccttgtc agttctagtt ttccatctaa 2040
aacagaaaca tccagtggaa gcggtcattt tcagcaccaa cagtttggaa gctttaagtg 2100
atttttatcc aggatatgtc ttttgttcta tggttcttgc agagtacttc ttggagcatt 2160
cgtacagctc catgcctata agagtaaacg tgtatatagc taccaaactc tgtgcgatga 2220
tcaggagacg tgttgccaac taaccagcat cttctctgcc cttgaggcat taatattttt 2280
ttacttatgg cgaaatggat atgttttgtg tacatgagta aagaaccaat gccatggtag 2340
caattgtaaa aagggttcat caattatgtg tgatacacgc tcatatgaat tttgaaaaat 2400
gaataacgca aagcattctg gatgctggca gtgcc 2435
<210>102
<211>628
<212>PRT
<213>稻
<400>102
Met Asp Phe Arg Lys Val Leu Asp Gln Thr Val Arg Glu Ile Arg Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly Ser Asp Leu
35 40 45
Ala Asp Ile Ala Arg Ala Thr Lys Ser Tyr Gly Asp Ser Glu Ile Ile
50 55 60
Met Asn Val Leu Trp Gln Arg Leu Gly Asn Thr Leu Ala Asn Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Ala Val Ile Glu Tyr Leu Leu Ala Asn Gly
85 90 95
Thr Glu Arg Ala Ala Asp Gly Ile Val Asp Asn Ser Ser Arg Ile Ala
100 105 110
Lys Leu Thr Arg Phe Glu Tyr Leu Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Leu Asn Val Arg Lys Lys Ala Glu Ala Val Leu Ala Ile Leu Asp Asp
130 135 140
Arg Glu Lys Leu Gln Glu Val Arg Glu Lys Ala Ala Val Thr Arg Asp
145 150 155 160
Lys Tyr Phe Gly Leu Ser Ser Thr Gly Ile Thr His Lys Ser Ser Ala
165 170 175
Ala Ser Phe Gly Ser Gly Ser Tyr Ser Ser Gly Ser His Tyr Gly Ser
180 185 190
Thr Gly Gly Ser Arg Glu Val Gly Ser Phe Lys Asp Ile His Thr Gly
195 200 205
Thr Glu Trp Lys Lys Asn Lys Lys Glu Thr Val Ser Asn Tyr Ser Ser
210 215 220
Asn Arg Glu Gly Ser Lys Glu Ile Thr Asn Ser Ala Thr Ser Tyr Lys
225 230 235 240
Ser Lys Lys Ser Glu Arg His Gly Arg Arg Asn Gln Asn Ser Leu Thr
245 250 255
Leu His Ser Lys Leu Ser Ala Asn Ile Ser Thr Thr Ser Glu Ala Pro
260 265 270
Ser Ser Lys Lys Gly Glu Asn Glu Asp Asp Asp Asp Phe Asn Pro Arg
275 280 285
Gly Phe Ser Thr Ser Thr Gly Thr Gly Thr Thr Arg Ser Asn His Leu
290 295 300
Asp Leu Phe Gly Pro Ser Leu Met Asp Asp Leu Val Asp Ser Thr Thr
305 310 315 320
Ser Thr Ser Thr Ala Thr Pro Asn Val Ser Thr Pro Ala Val Pro Glu
325 330 335
Val Asp Leu Phe Ala Asp Ala Ala Phe Gln Ser Ala Asn Ala Pro Leu
340 345 350
Glu Ala Ala Thr Val Ser His Thr Gln Asp Lys Ile Asp Leu Phe Ala
355 360 365
Gly Arg Leu Ser Ser Ala Asp Ser Phe Thr Ser Asp Thr Glu Phe Ser
370 375 380
Val Arg Gly Ser Pro Asn Lys Ser Ser Glu Lys Lys Met Ser Ser Val
385 390 395 400
Val His Pro Ser Thr Ser Ala Phe Asp Pro Phe Lys Gln Ser Phe Ala
405 410 415
Thr Ser Phe Pro Ser Asp Ser Glu Phe Ser Val His Asp Pro Thr Ser
420 425 430
Lys Ser Ser Gln Gly Lys Thr Pro Thr Pro Glu His Ser Ser Thr Ala
435 440 445
Ala Phe Asp Pro Phe Ala Ala Ile Pro Leu Lys Ser Phe Asp Gly Ser
450 455 460
Glu Ser Phe Gly Thr Phe Ser Ser Asn Thr Ala Ser Asn Ile Thr Glu
465 470 475 480
Leu Pro Arg Asp Ser Ser Gly Gly Pro Lys Ser Ser Asp His Gly Pro
485 490 495
Leu Glu Asp Ala Asn Phe Asp Ala Phe Thr Ser His Leu Gly Ser Ser
500 505 510
Thr Thr Ser Ala Thr Glu Ser Met Asn Lys Pro Ile Lys Lys Leu Gly
515 520 525
Gln Asp Ser Met Ser Ala Ser Lys Ser Val Ala Lys Lys Glu Thr Phe
530 535 540
Gln Val Lys Ser Gly Ile Trp Ala Asp Ser Leu Ser Arg Gly Leu Ile
545 550 555 560
Asp Leu Asn Ile Thr Ser Ser Gln Lys Lys Val Asp Leu Ser Asp Val
565 570 575
Gly Ile Val Gly Pro Leu Ser Gly Gly Ser Glu Asp Lys Gly Pro Gly
580 585 590
Ala Thr Met Gly Thr Ala Pro Gly Leu Val Ser Ser Ser Phe Pro Ser
595 600 605
Lys Thr Glu Thr Ser Ser Gly Ser Gly His Phe Gln His Gln Gln Phe
610 615 620
Gly Ser Phe Lys
625
<210>103
<211>602
<212>PRT
<213>稻
<400>103
Met Asp Phe Arg Lys Val Leu Asp Gln Thr Val Arg Glu Ile Arg Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly Ser Asp Leu
35 40 45
Ala Asp Ile Ala Arg Ala Thr Lys Ser Tyr Gly Asp Ser Glu Ile Ile
50 55 60
Met Asn Val Leu Trp Gln Arg Leu Gly Asn Thr Leu Ala Asn Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Ala Val Ile Glu Tyr Leu Leu Ala Asn Gly
85 90 95
Thr Glu Arg Ala Ala Asp Gly Ile Val Asp Asn Ser Ser Arg Ile Ala
100 105 110
Lys Leu Thr Arg Phe Glu Tyr Leu Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Leu Asn Val Arg Lys Lys Ala Glu Ala Val Leu Ala Ile Leu Asp Asp
130 135 140
Arg Glu Lys Leu Gln Glu Val Arg Glu Lys Ala Ala Val Thr Arg Asp
145 150 155 160
Lys Tyr Phe Gly Leu Ser Ser Thr Gly Ile Thr His Lys Ser Ser Ala
165 170 175
Ala Ser Phe Gly Ser Gly Ser Tyr Ser Ser Gly Ser His Tyr Gly Ser
180 185 190
Thr Gly Gly Ser Arg Glu Val Gly Ser Phe Lys Asp Ile His Thr Gly
195 200 205
Thr Glu Trp Lys Lys Asn Lys Lys Glu Thr Val Ser Asn Tyr Ser Ser
210 215 220
Asn Arg Glu Gly Ser Lys Glu Ile Thr Asn Ser Ala Thr Ser Tyr Lys
225 230 235 240
Ser Lys Lys Ser Glu Arg His Gly Arg Ser Thr Thr Ser Glu Ala Pro
245 250 255
Ser Ser Lys Lys Gly Glu Asn Glu Asp Asp Asp Asp Phe Asn Pro Arg
260 265 270
Gly Phe Ser Thr Ser Thr Thr Pro Asn Val Ser Thr Pro Ala Val Pro
275 280 285
Glu Val Asp Leu Phe Ala Asp Ala Ala Phe Gln Ser Ala Asn Ala Pro
290 295 300
Leu Glu Ala Ala Thr Val Ser His Thr Gln Asp Lys Ile Asp Leu Phe
305 310 315 320
Ala Gly Arg Leu Ser Ser Ala Asp Ser Phe Thr Ser Asp Thr Glu Phe
325 330 335
Ser Val Arg Gly Ser Pro Asn Lys Ser Ser Glu Lys Lys Met Ser Ser
340 345 350
Val Val His Pro Ser Thr Ser Ala Phe Asp Pro Phe Lys Gln Ser Phe
355 360 365
Ala Thr Ser Phe Pro Ser Asp Ser Glu Phe Ser Val His Asp Pro Thr
370 375 380
Ser Lys Ser Ser Gln Gly Lys Thr Pro Thr Pro Glu His Ser Ser Thr
385 390 395 400
Ala Ala Phe Asp Pro Phe Ala Ala Ile Pro Leu Lys Ser Phe Asp Gly
405 410 415
Ser Glu Ser Phe Gly Thr Phe Ser Ser Asn Thr Ala Ser Asn Ile Thr
420 425 430
Glu Leu Pro Arg Asp Ser Ser Gly Gly Pro Lys Ser Ser Asp His Gly
435 440 445
Pro Leu Glu Asp Ala Asn Phe Asp Ala Phe Thr Ser His Leu Gly Ser
450 455 460
Ser Thr Thr Ser Ala Thr Glu Ser Met Asn Lys Pro Ile Lys Lys Leu
465 470 475 480
Gly Gln Asp Ser Met Ser Ala Ser Lys Ser Val Ala Lys Lys Glu Thr
485 490 495
Phe Gln Val Lys Ser Gly Ile Trp Ala Asp Ser Leu Ser Arg Gly Leu
500 505 510
Ile Asp Leu Asn Ile Thr Ser Ser Gln Lys Lys Val Asp Leu Ser Asp
515 520 525
Val Gly Ile Val Gly Pro Leu Ser Gly Gly Ser Glu Asp Lys Gly Pro
530 535 540
Gly Ala Thr Met Gly Thr Ala Pro Gly Leu Val Ser Ser Ser Phe Pro
545 550 555 560
Ser Lys Thr Glu Thr Ser Ser Gly Ser Glu Tyr Phe Leu Glu His Ser
565 570 575
Tyr Ser Ser Met Pro Ile Arg Val Asn Val Tyr Ile Ala Thr Lys Leu
580 585 590
Cys Ala Met Ile Arg Arg Arg Val Ala Asn
595 600
<210>104
<211>618
<212>PRT
<213>稻
<400>104
Met Asp Phe Arg Lys Val Leu Asp Gln Thr Val Arg Glu Ile Arg Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly Ser Asp Leu
35 40 45
Ala Asp Ile Ala Arg Ala Thr Lys Ser Tyr Gly Asp Ser Glu Ile Ile
50 55 60
Met Asn Val Leu Trp Gln Arg Leu Gly Asn Thr Leu Ala Asn Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Ala Val Ile Glu Tyr Leu Leu Ala Asn Gly
85 90 95
Thr Glu Arg Ala Ala Asp Gly Ile Val Glu Asn Ser Ser Arg Ile Ala
100 105 110
Lys Leu Thr Arg Phe Glu Tyr Leu Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Leu Asn Val Arg Lys Lys Ala Glu Ala Val Leu Ala Ile Leu Asp Asp
130 135 140
Arg Glu Lys Leu Gln Glu Val Arg Glu Lys Ala Ala Val Thr Arg Asp
145 150 155 160
Lys Tyr Phe Gly Leu Ser Ser Thr Gly Ile Thr His Lys Ser Ser Ala
165 170 175
Ala Ser Phe Gly Ser Gly Ser Tyr Ser Ser Gly Ser His Tyr Gly Ser
180 185 190
Thr Gly Gly Ser Arg Glu Val Gly Ser Phe Lys Asp Ile His Thr Gly
195 200 205
Thr Glu Trp Lys Lys Asn Lys Lys Glu Thr Val Ser Asn Tyr Ser Ser
210 215 220
Asn Arg Glu Gly Ser Lys Glu Ile Thr Asn Ser Ala Thr Ser Tyr Lys
225 230 235 240
Ser Lys Lys Ser Glu Arg His Gly Arg Ser Leu Met Asp Asp Leu Val
245 250 255
Asp Ser Thr Thr Ser Thr Ser Thr Ala Thr Pro Asn Val Ser Thr Pro
260 265 270
Ala Val Pro Glu Val Asp Leu Phe Ala Asp Ala Ala Phe Gln Ser Ala
275 280 285
Asn Ala Pro Leu Glu Ala Ala Thr Val Ser His Thr Gln Asp Lys Ile
290 295 300
Asp Leu Phe Ala Gly Arg Leu Ser Ser Ala Asp Ser Phe Thr Ser Asp
305 310 315 320
Thr Glu Phe Ser Val Arg Gly Ser Pro Asn Lys Ser Ser Glu Lys Lys
325 330 335
Met Ser Ser Val Val His Pro Ser Thr Ser Ala Phe Asp Pro Phe Lys
340 345 350
Gln Ser Phe Ala Thr Ser Phe Pro Ser Asp Ala Glu Phe Ser Val His
355 360 365
Asp Pro Thr Ser Lys Ser Ser Gln Gly Lys Thr Pro Thr Pro Glu His
370 375 380
Ser Ser Thr Ala Ala Phe Asp Pro Phe Ala Ala Ile Pro Leu Lys Ser
385 390 395 400
Phe Asp Gly Ser Glu Ser Phe Gly Thr Phe Ser Ser Asn Thr Ala Ser
405 410 415
Asn Ile Thr Glu Leu Gln Arg Asp Ser Ser Gly Gly Pro Lys Ser Ser
420 425 430
Asp His Gly Pro Leu Glu Asp Ala Asn Phe Asp Ala Phe Thr Ser His
435 440 445
Leu Gly Ser Ser Thr Thr Ser Ala Thr Glu Ser Met Asn Lys Pro Ile
450 455 460
Lys Lys Leu Gly Gln Asp Ser Met Ser Ala Ser Lys Ser Val Ala Lys
465 470 475 480
Lys Glu Thr Phe Gln Val Lys Ser Gly Ile Trp Ala Asp Ser Leu Ser
485 490 495
Arg Gly Leu Ile Asp Leu Asn Ile Thr Ser Ser Gln Lys Lys Val Asp
500 505 510
Leu Ser Asp Val Gly Ile Val Gly Pro Leu Ser Gly Gly Ser Glu Asp
515 520 525
Lys Gly Pro Trp Tyr Met Gly Ala Thr Met Gly Thr Ala Pro Gly Leu
530 535 540
Val Ser Ser Ser Phe Pro Ser Lys Thr Glu Thr Ser Ser Gly Ser Ala
545 550 555 560
His Phe Gln His His Ser Leu Glu Ala Leu Ser Asp Phe Tyr Pro Gly
565 570 575
Tyr Val Phe Cys Ser Met Val Leu Ala Glu Tyr Phe Leu Glu His Ser
580 585 590
Tyr Ser Ser Met Pro Ile Arg Val Asn Val Tyr Ile Ala Thr Lys Leu
595 600 605
Ser Ala Met Ile Arg Arg Arg Val Ala Asn
610 615
<210>105
<211>2388
<212>DNA
<213>稻
<400>105
gggccatcgc ccaccggccc acgagcacca tctctcctct ctgctccgcg agtggccttt 60
tgcccaccta agccgcctcc ccatcctctg cttgctccgg cgggcggcga gcgccccgcc 120
ggaccgacga cgacctcgcc gtcgagctgg tggccgctca acctctagcg ctcgcaggga 180
tcccgggaag cctccctcgg ccgcgtcacc accggcatct ggtgctttcc gctcccacca 240
cagtgtgctg ctctcgtcag ttgccaccgc ccgactcggc gctccggagc ccagatcttg 300
gttccggagg atcctctccg gcgatggatt tcgtgaaggt tttcgatcag gccgtgcggg 360
agataaagag ggaggtcaat ttgaaggtgc tcaaggttcc agagctcgaa cagaaggtac 420
ttgatgcaac aagcgatgaa ccttggggcc ctcatggtac tactctttca gagctatcac 480
atgccaccaa gaagttcgct gaatgtcaga tggtaatgag tgtcctctgg actaggctgt 540
ctgagcgagg ctcgaaatgg cgtcacgtgt ataaggcttt gacaattatt gagtacttaa 600
tagccaatgg ttctgagcgg gcagttgatg acattcttga tcactattca aagatctcgg 660
ttctttcaag ttttgaatat gtggaaccta atggaaaaga tgctggaata aatgtgagaa 720
agaaagtcga aactatattg ggactcataa atgacaagga gaaaataaag tctgtaagag 780
agaaagccgc cagtaatcgt gacaagtatg ttgggctatc atcgacaggg ataacatata 840
agtcaagctc tgcttcattt ggtagcaact atagttctgg tgaacgttat gggagtttta 900
gtggtacaag ggaaggtgat tcatacggtg acagttatag ggataaagaa cctgttaaat 960
cctctccaag ttatactggc agccagaaat ctggcagcag gataaaaaag gatgtgaata 1020
gaaggaatga ggattcaccg agctccttga agtctaatgc aaaaggcaat gaggacgatt 1080
ttgatgattt tgatcctcgt ggatcttctt caaatggtgc agctaataca aacaccagtg 1140
gcgtggatct ttttgcccca aacttattgg atgatttcat cgatgtgcct gcagcagcaa 1200
ctcatgaaac aaacgactct gcagacgctc aggttgatct gtttgctgat gcagatttcc 1260
aatcagcaat accaagtaca gaaacagctg cgggttcaga tgtccagggc aatgtagacc 1320
tttttgcgga acagccagcc ttcacagcag cctttccacc acagacaggg tttattccac 1380
caccaagttc tgggacatct gaagcaaata cttccacgtc taagaacaca actcctgaac 1440
cttttgatcc ttttggtgct attcctataa acagctttga tggatctgat ccatttggtg 1500
ccttcaactc cgatgttgga tcatctagta taccaccacc cacgcagagt tctgttggaa 1560
atatcagcac accaagtcag aaccctcaag cagcatctga ctttggtggc tttgtgtcga 1620
gcactgtaga aacagcagct aaggacccct ttgatttttc aagtagcaat cttgggaaaa 1680
cacctttggc agatccaaag gctgatgcat ccgattttgg tgcctttgta tcgcacagtg 1740
aggaagtagc caaggatcct tttgatcttt ctttgagtac cagctctgga aggacaaacc 1800
aagcacctct ggcggctccc aagtcagata ccaagaaaga aaattttcag gtcaagtctg 1860
gcatatgggc tgactcattg agccgtggac tgattgatct gaacataact ggaccaaaga 1920
aggtgaactt agccgacgtt gggattgtcg gtggcctcga tgacgggtct gatgataaag 1980
ctctgccctc atggaccatg ggtgcaggag gttccagcct aggaatgtcc ggaattcctt 2040
catctacaca aagtggtggc attgagagct tagctaacta caacaagtac cagtttggct 2100
ttaaatgagc tttcatgtga ggctttctag tatatgccgg ttattgtcgg aatgtcggct 2160
tgtgccttat ttattttttg ttgattctat tctcccctct tttgatgctt acattccttt 2220
acaatcgcct ccaaggatcg tgatcttggt tcgtcttgtc gcgcttgtat tgtatttatt 2280
ggatttgcgg atgagagact cctacctata tcacaccctg attcaattta actgtactgt 2340
tatttcacaa tcgtgttaat cctatggctc acctctatat tgcgattt 2388
<210>106
<211>594
<212>PRT
<213>稻
<400>106
Met Asp Phe Val Lys Val Phe Asp Gln Ala Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Leu Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly Thr Thr Leu
35 40 45
Ser Glu Leu Ser His Ala Thr Lys Lys Phe Ala Glu Cys Gln Met Val
50 55 60
Met Ser Val Leu Trp Thr Arg Leu Ser Glu Arg Gly Ser Lys Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Thr Ile Ile Glu Tyr Leu Ile Ala Asn Gly
85 90 95
Ser Glu Arg Ala Val Asp Asp Ile Leu Asp His Tyr Ser Lys Ile Ser
100 105 110
Val Leu Ser Ser Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Ala Gly
115 120 125
Ile Asn Val Arg Lys Lys Val Glu Thr Ile Leu Gly Leu Ile Asn Asp
130 135 140
Lys Glu Lys Ile Lys Ser Val Arg Glu Lys Ala Ala Ser Asn Arg Asp
145 150 155 160
Lys Tyr Val Gly Leu Ser Ser Thr Gly Ile Thr Tyr Lys Ser Ser Ser
165 170 175
Ala Ser Phe Gly Ser Asn Tyr Ser Ser Gly Glu Arg Tyr Gly Ser Phe
180 185 190
Ser Gly Thr Arg Glu Gly Asp Ser Tyr Gly Asp Ser Tyr Arg Asp Lys
195 200 205
Glu Pro Val Lys Ser Ser Pro Ser Tyr Thr Gly Ser Gln Lys Ser Gly
210 215 220
Ser Arg Ile Lys Lys Asp Val Asn Arg Arg Asn Glu Asp Ser Pro Ser
225 230 235 240
Ser Leu Lys Ser Asn Ala Lys Gly Asn Glu Asp Asp Phe Asp Asp Phe
245 250 255
Asp Pro Arg Gly Ser Ser Ser Asn Gly Ala Ala Asn Thr Asn Thr Ser
260 265 270
Gly Val Asp Leu Phe Ala Pro Asn Leu Leu Asp Asp Phe Ile Asp Val
275 280 285
Pro Ala Ala Ala Thr His Glu Thr Asn Asp Ser Ala Asp Ala Gln Val
290 295 300
Asp Leu Phe Ala Asp Ala Asp Phe Gln Ser Ala Ile Pro Ser Thr Glu
305 310 315 320
Thr Ala Ala Gly Ser Asp Val Gln Gly Asn Val Asp Leu Phe Ala Glu
325 330 335
Gln Pro Ala Phe Thr Ala Ala Phe Pro Pro Gln Thr Gly Phe Ile Pro
340 345 350
Pro Pro Ser Ser Gly Thr Ser Glu Ala Asn Thr Ser Thr Ser Lys Asn
355 360 365
Thr Thr Pro Glu Pro Phe Asp Pro Phe Gly Ala Ile Pro Ile Asn Ser
370 375 380
Phe Asp Gly Ser Asp Pro Phe Gly Ala Phe Asn Ser Asp Val Gly Ser
385 390 395 400
Ser Ser Ile Pro Pro Pro Thr Gln Ser Ser Val Gly Asn Ile Ser Thr
405 410 415
Pro Ser Gln Asn Pro Gln Ala Ala Ser Asp Phe Gly Gly Phe Val Ser
420 425 430
Ser Thr Val Glu Thr Ala Ala Lys Asp Pro Phe Asp Phe Ser Ser Ser
435 440 445
Asn Leu Gly Lys Thr Pro Leu Ala Asp Pro Lys Ala Asp Ala Ser Asp
450 455 460
Phe Gly Ala Phe Val Ser His Ser Glu Glu Val Ala Lys Asp Pro Phe
465 470 475 480
Asp Leu Ser Leu Ser Thr Ser Ser Gly Arg Thr Asn Gln Ala Pro Leu
485 490 495
Ala Ala Pro Lys Ser Asp Thr Lys Lys Glu Asn Phe Gln Val Lys Ser
500 505 510
Gly Ile Trp Ala Asp Ser Leu Ser Arg Gly Leu Ile Asp Leu Asn Ile
515 520 525
Thr Gly Pro Lys Lys Val Asn Leu Ala Asp Val Gly Ile Val Gly Gly
530 535 540
Leu Asp Asp Gly Ser Asp Asp Lys Ala Leu Pro Ser Trp Thr Met Gly
545 550 555 560
Ala Gly Gly Ser Ser Leu Gly Met Ser Gly Ile Pro Ser Ser Thr Gln
565 570 575
Ser Gly Gly Ile Glu Ser Leu Ala Asn Tyr Asn Lys Tyr Gln Phe Gly
580 585 590
Phe Lys
<210>107
<211>593
<212>PRT
<213>稻
<400>107
Met Asp Phe Val Lys Val Phe Asp Gln Ala Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Leu Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly Thr Thr Leu
35 40 45
Ser Glu Leu Ser His Ala Thr Lys Lys Phe Thr Gly Ala Leu Tyr Tyr
50 55 60
Glu Phe Tyr Ile Gly His Tyr Ala Asn Thr Asn Gly Gln Ser Glu Gln
65 70 75 80
Gln Thr Leu Ala Leu Thr Ile Ile Glu Tyr Leu Ile Ala Asn Gly Ser
85 90 95
Glu Arg Ala Val Asp Asp Ile Leu Asp His Tyr Ser Lys Ile Ser Val
100 105 110
Leu Ser Ser Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Ala Gly Ile
115 120 125
Asn Val Arg Lys Lys Val Glu Thr Ile Leu Gly Leu Ile Asn Asp Lys
130 135 140
Glu Lys Ile Lys Ser Val Arg Glu Lys Ala Ala Ser Asn Arg Asp Lys
145 150 155 160
Tyr Val Gly Leu Ser Ser Thr Gly Ile Thr Tyr Lys Ser Ser Ser Ala
165 170 175
Ser Phe Gly Ser Asn Tyr Ser Ser Gly Glu Arg Tyr Gly Ser Phe Ser
180 185 190
Gly Thr Arg Glu Gly Asp Ser Tyr Gly Asp Ser Tyr Arg Asp Lys Glu
195 200 205
Pro Val Lys Ser Ser Pro Ser Tyr Thr Gly Ser Gln Lys Ser Gly Ser
210 215 220
Arg Ile Lys Lys Asp Val Asn Arg Arg Asn Glu Asp Ser Pro Ser Ser
225 230 235 240
Leu Lys Ser Asn Ala Lys Gly Asn Glu Asp Asp Phe Asp Asp Phe Asp
245 250 255
Pro Arg Gly Ser Ser Ser Asn Gly Ala Ala Asn Thr Asn Thr Ser Gly
260 265 270
Val Asp Leu Phe Ala Pro Asn Leu Leu Asp Asp Phe Ile Asp Val Pro
275 280 285
Ala Ala Ala Thr His Glu Thr Asn Asp Ser Ala Asp Ala Gln Val Asp
290 295 300
Leu Phe Ala Asp Ala Asp Phe Gln Ser Ala Ile Pro Ser Thr Glu Thr
305 310 315 320
Ala Ala Gly Ser Asp Val Gln Gly Asn Val Asp Leu Phe Ala Glu Gln
325 330 335
Pro Ala Phe Thr Ala Ala Phe Pro Pro Gln Thr Gly Phe Ile Pro Pro
340 345 350
Pro Ser Ser Gly Thr Ser Glu Ala Asn Thr Ser Thr Ser Lys Asn Thr
355 360 365
Thr Pro Glu Pro Phe Asp Pro Phe Gly Ala Ile Pro Ile Asn Ser Phe
370 375 380
Asp Gly Ser Asp Pro Phe Gly Ala Phe Asn Ser Asp Val Gly Ser Ser
385 390 395 400
Ser Ile Pro Pro Pro Thr Gln Ser Ser Val Gly Asn Ile Ser Thr Pro
405 410 415
Ser Gln Asn Pro Gln Ala Ala Ser Asp Phe Gly Gly Phe Val Ser Ser
420 425 430
Thr Val Glu Thr Ala Ala Lys Asp Pro Phe Asp Phe Ser Ser Ser Asn
435 440 445
Leu Gly Lys Thr Pro Leu Ala Asp Pro Lys Ala Asp Ala Ser Asp Phe
450 455 460
Gly Ala Phe Val Ser His Ser Glu Glu Val Ala Lys Asp Pro Phe Asp
465 470 475 480
Leu Ser Leu Ser Thr Ser Ser Gly Arg Thr Asn Gln Ala Pro Leu Ala
485 490 495
Ala Pro Lys Ser Asp Thr Lys Lys Glu Asn Phe Gln Val Lys Ser Gly
500 505 510
Ile Trp Ala Asp Ser Leu Ser Arg Gly Leu Ile Asp Leu Asn Ile Thr
515 520 525
Gly Pro Lys Lys Val Asn Leu Ala Asp Val Gly Ile Val Gly Gly Leu
530 535 540
Asp Asp Gly Ser Asp Asp Lys Ala Leu Pro Ser Trp Thr Met Gly Ala
545 550 555 560
Gly Gly Ser Ser Leu Gly Met Ser Gly Ile Pro Ser Ser Thr Gln Ser
565 570 575
Gly Gly Ile Glu Ser Leu Ala Asn Tyr Asn Lys Tyr Gln Phe Gly Phe
580 585 590
Lys
<210>108
<211>3750
<212>DNA
<213>稻
<400>108
gtcagggaag aagggaggac gcgacgcgac acgagcgcga gaggaaagcg gaggagatcg 60
aggagaaact ccatttcccc ccccctcgcc ttctccgatt tccctcgcgt ggaatcccct 120
tggactcctc cccctcccgc cgccgcgcga gccgggcacg gcgagcggcg ccgccctgcc 180
cccgccgatc tgctctgcct tctcctcagg ggcttgcacc gaggaggagg aggaggagag 240
agctgagcag aacaagctag attctcaagg aagggcgcca tgaagaaagt attcgatcaa 300
acagtccggg acctaaaaag agaggtgaac aagaaggtcc tcaaggtgcc tggtatagaa 360
caaaagattc ttgatgccac aagcaacgag ccatggggcc cacatggatc actcttggca 420
gaaatcgccc aagcaacaca aaattaccat gagtaccaga tggtcatgaa tgttgtgtgg 480
aagaggatca atgacactgg taaaaattgg cggcatgttt ataagggatt gattgtcctg 540
gattacttgg ttgctcatgg tacagagcga gttattgatg acataaggga acattcttat 600
cagatatcga cactagctga tttccagtat atcgattcga gtgggagaga tcaaggtagc 660
aatgtacggc ggaaatccca gagccttgtt agtttagtta atgacaaaga gaggatacag 720
gaagttaggc agaaagcact tgctaccagg gacaaatacc ggagtgcatt cgccaccagt 780
ggaacacaca ggagcccagg tggctatgac aatgaccgct atgaaggaag ctatggcagt 840
aggtatgata acagaaatgg ctacggggga gaaagagagt atggatatag ggatgatgac 900
agatatggtg ttgctggaac tactcccaat cgggaaggag atcgttattc tagggattct 960
aatgagcagc gctacagcag agatagagaa gatgagtaca aaggaagtca tagcaaccat 1020
gaatatgcag aaggatcagg tcgcaggagc tatggacgag atagggattc atatggggat 1080
gatgaagctt attcatcccg tggccggcaa agcaatgctg acggacctac tcaggatgag 1140
aggcccatgg agcggaagcc ttctaaccag cagattgctt caccaccgcc aaactatgaa 1200
gatgtcacaa gagatacaca ggataataat catgatggac gaaatggagg gactgtgcct 1260
gtgccagttg ctgcagcaaa ggtgtcttca cctccaagaa caagtgtacc cccaggtcag 1320
gtgaatggtg tacatgataa tactgtggaa gacgtacctg caccaccacc tactcatcct 1380
gaagttaatg ggtttgacga gtttgatcct cgtggatcag taccagatac ctcacctcca 1440
gtgaacccct cacaagccgt gaatagcttg gaaatggatt tattcggacc agatcctatt 1500
aattcgttgg ctttggtatc tgtgcctcag ccaactgcca gcccaaatgt tgagccatca 1560
gcaaatccag gatttgagag taacagtttt atgggcatgc caccggcttc tactggattc 1620
aatgaggcct ttgatgcaac taatcctttt ggggatccta cgcctttcaa ggctgttcat 1680
gaagaaactc ctgcagtttc tcagacaaat gctgcacctg ctggttcttt ccatgctact 1740
gaacctgctg cagatgcaaa tcctttccag cctgcttcag cagcaagctt tggttttgga 1800
gacactcttg gcgacctctc ttttggatcg aatgctgcac ccgggcagca agatattttt 1860
gtgcccacct catcacattc agaggtacca cctgcaaacc catctgtgca ccctgaacag 1920
gcagtcccat cttatgtctc ctcccaggca cctcagcctg cagctgctgg tccacaaact 1980
catgctgctc ccgcttcatt tgcttctcaa gcacctccca cttcatttgc ttctcaagca 2040
cctcaagcag gggcacccta tcctcaggct gcctccacat ttcctcattc acaagcatct 2100
catcctgcag ccactaatcc atctacaatc cctcagaatg ttgccacacc atttgctcct 2160
ttacaaatgc ctcaacctgt accatcaggc caatcaaact attttatgca accagttcca 2220
gggactggca ttaatggcat gtctggggct ccttcacaga atggagcacc atcctacatc 2280
ccttcacagg cttctcaatt tgcagctcca acaaatctac agccctctca gccaaccttc 2340
cccccacaaa ctgccatggc agcttcacag gcaacatcaa tttctcgagg ggcatctcag 2400
cctcttgcag tgccgaattc aatgccttct ggtgtgaact ttccattaca atcaagttca 2460
tcagctcccc ctgaaacaat cctttcagcc ttgcaagtta gtcagtctga gccagtgaag 2520
aaatttgagt ccaaatctac agtttgggct gatacattga gccgaggttt agtcaatctg 2580
gacatctctg gaccaaaggc taatccgcac gctgatattg gagttgactt cgattcaatc 2640
aatcgcaagg agaaaaggca agaaaagaaa gtctctcaag ctcctgtggt atctacgatc 2700
actatgggca aagccatggg aactggctcc ggcatcggtc gggcaggtgc gagtgccatg 2760
gcacctccag ccaacccaat gggtgcaagc aggggcattg gcatgggtat gggcgctgct 2820
ggttccggtt acggtggtgg aatgggaatg aaccggccaa tgggcatggg aatgggaatg 2880
aaccaacaaa tgggtatggg aatggggatg aaccaacagg ccatgggaat ggggatgaac 2940
caacaggcca tgggaatggg gatgaaccaa caaccaatgg gaatgaacat gggcatgggg 3000
atgaaccagg gtatgggaat gaacatgcgg cctcctatgg gaatgggtcc aggtagtgga 3060
tataatccaa tgggtaccgg atacggaggg cagcaaccat atggtgggta caggtgagat 3120
tatactgtac aatgcgatac tcagtgaggg gttgagaaac agtttaggag gcgtttggat 3180
tttggtcacc tggattttct ttatgtatgg tttcttttgt tttgggtggg gggggggggt 3240
tataattttt catcactatt cttttcctat ccatgttgga tattgtatct actaaatata 3300
tgacatgtga acgggggcaa tgctacaact actgtgttgt gttggatgca aagccttaat 3360
actataatca tcaatgtagc tgtataattc tctataggat gttttttttt cagtaaacat 3420
tgtgattgtt tcagcgttct tgctggcttg acaataaaaa ggctgtacca ttttgttcaa 3480
gctatcttcc tttcttttta tgtcgtcaca ttttgaggtg cagacctgca gtttttgtgt 3540
cagatgtcac ccgtcagtct gcaaatagta gtatattttt gctcgtccga actttggaac 3600
tacaggaatg gcccatctct gctgtcgcct cttttctgtt ctgatgcgtg gtcacacaag 3660
ccactgttga cacagcaact ttgtatctcc attattgaca cagcaacttt gtatctgatc 3720
ataatgaaac gaagaatttt gtgagttcaa 3750
<210>109
<211>945
<212>PRT
<213>稻
<400>109
Met Lys Lys Val Phe Asp Gln Thr Val Arg Asp Leu Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Ile Glu Gln Lys Ile Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser Leu Leu Ala Glu
35 40 45
Ile Ala Gln Ala Thr Gln Asn Tyr His Glu Tyr Gln Met Val Met Asn
50 55 60
Val Val Trp Lys Arg Ile Asn Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Gly Leu Ile Val Leu Asp Tyr Leu Val Ala His Gly Thr Glu
85 90 95
Arg Val Ile Asp Asp Ile Arg Glu His Ser Tyr Gln Ile Ser Thr Leu
100 105 110
Ala Asp Phe Gln Tyr Ile Asp Ser Ser Gly Arg Asp Gln Gly Ser Asn
115 120 125
Val Arg Arg Lys Ser Gln Ser Leu Val Ser Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Gln Glu Val Arg Gln Lys Ala Leu Ala Thr Arg Asp Lys Tyr
145 150 155 160
Arg Ser Ala Phe Ala Thr Ser Gly Thr His Arg Ser Pro Gly Gly Tyr
165 170 175
Asp Asn Asp Arg Tyr Glu Gly Ser Tyr Gly Ser Arg Tyr Asp Asn Arg
180 185 190
Asn Gly Tyr Gly Gly Glu Arg Glu Tyr Gly Tyr Arg Asp Asp Asp Arg
195 200 205
Tyr Gly Val Ala Gly Thr Thr Pro Asn Arg Glu Gly Asp Arg Tyr Ser
210 215 220
Arg Asp Ser Asn Glu Gln Arg Tyr Ser Arg Asp Arg Glu Asp Glu Tyr
225 230 235 240
Lys Gly Ser His Ser Asn His Glu Tyr Ala Glu Gly Ser Gly Arg Arg
245 250 255
Ser Tyr Gly Arg Asp Arg Asp Ser Tyr Gly Asp Asp Glu Ala Tyr Ser
260 265 270
Ser Arg Gly Arg Gln Ser Asn Ala Asp Gly Pro Thr Gln Asp Glu Arg
275 280 285
Pro Met Glu Arg Lys Pro Ser Asn Gln Gln Ile Ala Ser Pro Pro Pro
290 295 300
Asn Tyr Glu Asp Val Thr Arg Asp Thr Gln Asp Asn Asn His Asp Gly
305 310 315 320
Arg Asn Gly Gly Thr Val Pro Val Pro Val Ala Ala Ala Lys Val Ser
325 330 335
Ser Pro Pro Arg Thr Ser Val Pro Pro Gly Gln Val Asn Gly Val His
340 345 350
Asp Asn Thr Val Glu Asp Val Pro Ala Pro Pro Pro Thr His Pro Glu
355 360 365
Val Asn Gly Phe Asp Glu Phe Asp Pro Arg Gly Ser Val Pro Asp Thr
370 375 380
Ser Pro Pro Val Asn Pro Ser Gln Ala Val Asn Ser Leu Glu Met Asp
385 390 395 400
Leu Phe Gly Pro Asp Pro Ile Asn Ser Leu Ala Leu Val Ser Val Pro
405 410 415
Gln Pro Thr Ala Ser Pro Asn Val Glu Pro Ser Ala Asn Pro Gly Phe
420 425 430
Glu Ser Asn Ser Phe Met Gly Met Pro Pro Ala Ser Thr Gly Phe Asn
435 440 445
Glu Ala Phe Asp Ala Thr Asn Pro Phe Gly Asp Pro Thr Pro Phe Lys
450 455 460
Ala Val His Glu Glu Thr Pro Ala Val Ser Gln Thr Asn Ala Ala Pro
465 470 475 480
Ala Gly Ser Phe His Ala Thr Glu Pro Ala Ala Asp Ala Asn Pro Phe
485 490 495
Gln Pro Ala Ser Ala Ala Ser Phe Gly Phe Gly Asp Thr Leu Gly Asp
500 505 510
Leu Ser Phe Gly Ser Asn Ala Ala Pro Gly Gln Gln Asp Ile Phe Val
515 520 525
Pro Thr Ser Ser His Ser Glu Val Pro Pro Ala Asn Pro Ser Val His
530 535 540
Pro Glu Gln Ala Val Pro Ser Tyr Val Ser Ser Gln Ala Pro Gln Pro
545 550 555 560
Ala Ala Ala Gly Pro Gln Thr His Ala Ala Pro Ala Ser Phe Ala Ser
565 570 575
Gln Ala Pro Pro Thr Ser Phe Ala Ser Gln Ala Pro Gln Ala Gly Ala
580 585 590
Pro Tyr Pro Gln Ala Ala Ser Thr Phe Pro His Ser Gln Ala Ser His
595 600 605
Pro Ala Ala Thr Asn Pro Ser Thr Ile Pro Gln Asn Val Ala Thr Pro
610 615 620
Phe Ala Pro Leu Gln Met Pro Gln Pro Val Pro Ser Gly Gln Ser Asn
625 630 635 640
Tyr Phe Met Gln Pro Val Pro Gly Thr Gly Ile Asn Gly Met Ser Gly
645 650 655
Ala Pro Ser Gln Asn Gly Ala Pro Ser Tyr Ile Pro Ser Gln Ala Ser
660 665 670
Gln Phe Ala Ala Pro Thr Asn Leu Gln Pro Ser Gln Pro Thr Phe Pro
675 680 685
Pro Gln Thr Ala Met Ala Ala Ser Gln Ala Thr Ser Ile Ser Arg Gly
690 695 700
Ala Ser Gln Pro Leu Ala Val Pro Asn Ser Met Pro Ser Gly Val Asn
705 710 715 720
Phe Pro Leu Gln Ser Ser Ser Ser Ala Pro Pro Glu Thr Ile Leu Ser
725 730 735
Ala Leu Gln Val Ser Gln Ser Glu Pro Val Lys Lys Phe Glu Ser Lys
740 745 750
Ser Thr Val Trp Ala Asp Thr Leu Ser Arg Gly Leu Val Asn Leu Asp
755 760 765
Ile Ser Gly Pro Lys Ala Asn Pro His Ala Asp Ile Gly Val Asp Phe
770 775 780
Asp Ser Ile Asn Arg Lys Glu Lys Arg Gln Glu Lys Lys Val Ser Gln
785 790 795 800
Ala Pro Val Val Ser Thr Ile Thr Met Gly Lys Ala Met Gly Thr Gly
805 810 815
Ser Gly Ile Gly Arg Ala Gly Ala Ser Ala Met Ala Pro Pro Ala Asn
820 825 830
Pro Met Gly Ala Ser Arg Gly Ile Gly Met Gly Met Gly Ala Ala Gly
835 840 845
Ser Gly Tyr Gly Gly Gly Met Gly Met Asn Arg Pro Met Gly Met Gly
850 855 860
Met Gly Met Asn Gln Gln Met Gly Met Gly Met Gly Met Asn Gln Gln
865 870 875 880
Ala Met Gly Met Gly Met Asn Gln Gln Ala Met Gly Met Gly Met Asn
885 890 895
Gln Gln Pro Met Gly Met Asn Met Gly Met Gly Met Asn Gln Gly Met
900 905 910
Gly Met Asn Met Arg Pro Pro Met Gly Met Gly Pro Gly Ser Gly Tyr
915 920 925
Asn Pro Met Gly Thr Gly Tyr Gly Gly Gln Gln Pro Tyr Gly Gly Tyr
930 935 940
Arg
945
<210>110
<211>945
<212>PRT
<213>稻
<400>110
Met Lys Lys Val Phe Asp Gln Thr Val Arg Asp Leu Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Ile Glu Gln Lys Ile Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser Leu Leu Ala Glu
35 40 45
Ile Ala Gln Ala Thr Gln Asn Tyr His Glu Tyr Gln Met Val Met Asn
50 55 60
Val Val Trp Lys Arg Ile Asn Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Gly Leu Ile Val Leu Asp Tyr Leu Val Ala His Gly Thr Glu
85 90 95
Arg Val Ile Asp Asp Ile Arg Glu His Ser Tyr Gln Ile Ser Thr Leu
100 105 110
Ala Asp Phe Gln Tyr Ile Asp Ser Ser Gly Arg Asp Gln Gly Ser Asn
115 120 125
Val Arg Arg Lys Ser Gln Ser Leu Val Ser Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Gln Glu Val Arg Gln Lys Ala Leu Ala Thr Arg Asp Lys Tyr
145 150 155 160
Arg Ser Ala Phe Ala Thr Ser Gly Thr His Arg Ser Pro Gly Gly Tyr
165 170 175
Asp Asn Asp Arg Tyr Glu Gly Ser Tyr Gly Ser Arg Tyr Asp Asn Arg
180 185 190
Asn Gly Tyr Gly Gly Glu Arg Glu Tyr Gly Tyr Arg Asp Asp Asp Arg
195 200 205
Tyr Gly Val Ala Gly Thr Thr Pro Asn Arg Glu Gly Asp Arg Tyr Ser
210 215 220
Arg Asp Ser Asn Glu Gln Arg Tyr Ser Arg Asp Arg Glu Asp Glu Tyr
225 230 235 240
Lys Gly Ser His Ser Asn His Glu Tyr Ala Glu Gly Ser Gly Arg Arg
245 250 255
Ser Tyr Gly Arg Asp Arg Asp Ser Tyr Gly Asp Asp Glu Ala Tyr Ser
260 265 270
Ser Arg Gly Arg Gln Ser Asn Ala Asp Gly Pro Thr Gln Asp Glu Arg
275 280 285
Pro Met Glu Arg Lys Pro Ser Asn Gln Gln Ile Ala Ser Pro Pro Pro
290 295 300
Asn Tyr Glu Asp Val Thr Arg Asp Thr Gln Asp Asn Asn His Asp Gly
305 310 315 320
Arg Asn Gly Gly Thr Val Pro Val Pro Val Ala Ala Ala Lys Val Ser
325 330 335
Ser Pro Pro Arg Thr Ser Val Pro Pro Gly Gln Val Asn Gly Val His
340 345 350
Asp Asn Thr Val Glu Asp Val Pro Ala Pro Pro Pro Thr His Pro Glu
355 360 365
Val Asn Gly Phe Asp Glu Phe Asp Pro Arg Gly Ser Val Pro Asp Thr
370 375 380
Ser Pro Pro Val Asn Pro Ser Gln Ala Val Asn Ser Leu Glu Met Asp
385 390 395 400
Leu Phe Gly Pro Asp Pro Ile Asn Ser Leu Ala Leu Val Ser Val Pro
405 410 415
Gln Pro Thr Ala Ser Pro Asn Val Glu Pro Ser Ala Asn Pro Gly Phe
420 425 430
Glu Ser Asn Ser Phe Met Gly Met Pro Pro Ala Ser Thr Gly Phe Asn
435 440 445
Glu Ala Phe Asp Ala Thr Asn Pro Phe Gly Asp Pro Thr Pro Phe Lys
450 455 460
Ala Val His Glu Glu Thr Pro Ala Val Ser Gln Thr Asn Ala Ala Pro
465 470 475 480
Ala Gly Ser Phe His Ala Thr Glu Pro Ala Ala Asp Ala Asn Pro Phe
485 490 495
Gln Pro Ala Ser Ala Ala Ser Phe Gly Phe Gly Asp Thr Leu Gly Asp
500 505 510
Leu Ser Phe Gly Ser Asn Ala Ala Pro Gly Gln Gln Asp Ile Phe Val
515 520 525
Pro Thr Ser Ser His Ser Glu Val Pro Pro Ala Asn Pro Ser Val His
530 535 540
Pro Glu Gln Ala Val Pro Ser Tyr Val Ser Ser Gln Ala Pro Gln Pro
545 550 555 560
Ala Ala Ala Gly Pro Gln Thr His Ala Ala Pro Ala Ser Phe Ala Ser
565 570 575
Gln Ala Pro Pro Thr Ser Phe Ala Ser Gln Ala Pro Gln Ala Gly Ala
580 585 590
Pro Tyr Pro Gln Ala Ala Ser Thr Phe Pro His Ser Gln Ala Ser His
595 600 605
Pro Ala Ala Thr Asn Pro Ser Thr Ile Pro Gln Asn Val Ala Thr Pro
610 615 620
Phe Ala Pro Leu Gln Met Pro Gln Pro Val Pro Ser Gly Gln Ser Asn
625 630 635 640
Tyr Phe Met Gln Pro Val Pro Gly Thr Gly Ile Asn Gly Met Ser Gly
645 650 655
Ala Pro Ser Gln Asn Gly Ala Pro Ser Tyr Ile Pro Ser Gln Ala Ser
660 665 670
Gln Phe Ala Ala Pro Thr Asn Leu Gln Pro Ser Gln Pro Thr Phe Pro
675 680 685
Pro Gln Thr Ala Met Ala Ala Ser Gln Ala Thr Ser Ile Ser Arg Gly
690 695 700
Ala Ser Gln Pro Leu Ala Val Pro Asn Ser Met Pro Ser Gly Val Asn
705 710 715 720
Phe Pro Leu Gln Ser Ser Ser Ser Ala Pro Pro Glu Thr Ile Leu Ser
725 730 735
Ala Leu Gln Val Ser Gln Ser Glu Pro Val Lys Lys Phe Glu Ser Lys
740 745 750
Ser Thr Val Trp Ala Asp Thr Leu Ser Arg Gly Leu Val Asn Leu Asp
755 760 765
Ile Ser Gly Pro Lys Ala Asn Pro His Ala Asp Ile Gly Val Asp Phe
770 775 780
Asp Ser Ile Asn Arg Lys Glu Lys Arg Gln Glu Lys Lys Val Ser Gln
785 790 795 800
Ala Pro Val Val Ser Thr Ile Thr Met Gly Lys Ala Met Gly Thr Gly
805 810 815
Ser Gly Ile Gly Arg Ala Gly Ala Ser Ala Met Ala Pro Pro Ala Asn
820 825 830
Pro Met Gly Ala Ser Arg Gly Ile Gly Met Gly Met Gly Ala Ala Gly
835 840 845
Ser Gly Tyr Gly Gly Gly Met Gly Met Asn Arg Pro Met Gly Met Gly
850 855 860
Met Gly Met Asn Gln Gln Met Gly Met Gly Met Gly Met Asn Gln Gln
865 870 875 880
Ala Met Gly Met Gly Ile Asn Gln Gln Ala Met Gly Met Gly Met Asn
885 890 895
Gln Gln Pro Met Gly Met Asn Met Gly Met Gly Met Asn Gln Gly Met
900 905 910
Gly Met Asn Met Arg Pro Pro Met Gly Met Gly Pro Gly Ser Gly Tyr
915 920 925
Asn Pro Met Gly Thr Gly Tyr Gly Gly Gln Gln Pro Tyr Gly Gly Tyr
930 935 940
Arg
945
<210>111
<211>1356
<212>DNA
<213>欧洲油菜(Brassica napus)
<400>111
ttcccgggtc gacgatatga gattattgag catacttacc aaatctctgt cagtatttct 60
tttcttatat atttataagt cattttcctg tggtggaacc ttgtgacatg atctttgttt 120
gtgagttgtt acagtcactc acgagttttg agtatgttga accgaatggg aaagatgtgg 180
ggatcaatgt gagaaagaag gcggaaaaca tagttgctct cttgaataac aaggagaaga 240
tctctcaaat cagagacaaa gcaacagcca atcgtaacaa gtacgttggc ctctcctcaa 300
caggaataac atataagtct ggttcagctt cagcttcgtt tggtggtggt agtggttcaa 360
gcaattatga cagctataga gatagggatt ccagagaaga caaggacgac tacgagtctt 420
ttcaaaagtc aaggcgcggt gttaaaggtg aagaccaaag ctacacttcg aagaaaagtt 480
tttcacgcta tggcagtgcg gaccaggata gtctatccag tggaaaaaag tctcccggct 540
ctggcaaaca cagtgccata ccttcacatg catctgcagc tccttcaaac aacgatgatg 600
attttgatga ttttgatcca cgtggaactt ccagcaagaa gccttccgcg gggagtgcca 660
accaagtgga cctttttgga gcagatctga ttggtgactt cttggattct gggccaactg 720
aaacatcttc caccaacaac aatgggaagt ttcaagaggc ggacttgttt gctgatgcag 780
attttgtatc agcctccagt ccaagaacag actttgggtc acaggcacag tttcttcagc 840
tcctgcgacg gttgatcttt ttgcatctcc ggaaacagtt gcacgcccag aagccaagat 900
tcctaaacct gagccgatca ccactccaag cattgttgac ccatttgctg cagttcctat 960
ggaaaatttt gatggatctg atccttttgg tgccttcact tctcactcgg cttcagtctc 1020
tacaggtcca caggccccag ttctacatgg gagtgcaaca aacacaacaa ccccaccctc 1080
tttggcagac tcgaaaccac aacagtttca aaagaaggat cctttccagg tgaaatctgg 1140
aatatgggca gattcgctga gccgtggact aattgatctc aatattactg ctcccaaaaa 1200
ggcttctctt gctgatgtgg gtgttgtcgg tggcctgagc aatgacgatg ggaacaaggc 1260
atctgctgct gcctactact cagggtggtc catgggcgca ggatccgggc tcggtaaaac 1320
cggaatatac aatgcccagc agcagcagca acaaca 1356
<210>112
<211>341
<212>PRT
<213>欧洲油菜
<400>112
Met Ile Phe Val Cys Glu Leu Leu Gln Ser Leu Thr Ser Phe Glu Tyr
1 5 10 15
Val Glu Pro Asn Gly Lys Asp Val Gly Ile Asn Val Arg Lys Lys Ala
20 25 30
Glu Asn Ile Val Ala Leu Leu Asn Asn Lys Glu Lys Ile Ser Gln Ile
35 40 45
Arg Asp Lys Ala Thr Ala Asn Arg Asn Lys Tyr Val Gly Leu Ser Ser
50 55 60
Thr Gly Ile Thr Tyr Lys Ser Gly Ser Ala Ser Ala Ser Phe Gly Gly
65 70 75 80
Gly Ser Gly Ser Ser Asn Tyr Asp Ser Tyr Arg Asp Arg Asp Ser Arg
85 90 95
Glu Asp Lys Asp Asp Tyr Glu Ser Phe Gln Lys Ser Arg Arg Gly Val
100 105 110
Lys Gly Glu Asp Gln Ser Tyr Thr Ser Lys Lys Ser Phe Ser Arg Tyr
115 120 125
Gly Ser Ala Asp Gln Asp Ser Leu Ser Ser Gly Lys Lys Ser Pro Gly
130 135 140
Ser Gly Lys His Ser Ala Ile Pro Ser His Ala Ser Ala Ala Pro Ser
145 150 155 160
Asn Asn Asp Asp Asp Phe Asp Asp Phe Asp Pro Arg Gly Thr Ser Ser
165 170 175
Lys Lys Pro Ser Ala Gly Ser Ala Asn Gln Val Asp Leu Phe Gly Ala
180 185 190
Asp Leu Ile Gly Asp Phe Leu Asp Ser Gly Pro Thr Glu Thr Ser Ser
195 200 205
Thr Asn Asn Asn Gly Lys Phe Gln Glu Ala Asp Leu Phe Ala Asp Ala
210 215 220
Asp Phe Val Ser Ala Ser Ser Pro Arg Thr Asp Phe Gly Ser Gln Ala
225 230 235 240
Gln Phe Leu Gln Leu Leu Arg Arg Leu Ile Phe Leu His Leu Arg Lys
245 250 255
Gln Leu His Ala Gln Lys Pro Arg Phe Leu Asn Leu Ser Arg Ser Pro
260 265 270
Leu Gln Ala Leu Leu Thr His Leu Leu Gln Phe Leu Trp Lys Ile Leu
275 280 285
Met Asp Leu Ile Leu Leu Val Pro Ser Leu Leu Thr Arg Leu Gln Ser
290 295 300
Leu Gln Val His Arg Pro Gln Phe Tyr Met Gly Val Gln Gln Thr Gln
305 310 315 320
Gln Pro His Pro Leu Trp Gln Thr Arg Asn His Asn Ser Phe Lys Arg
325 330 335
Arg Ile Leu Ser Arg
340
<210>113
<211>2051
<212>DNA
<213>大豆(Glycine max)
<400>113
tataatcaca atattaggag aaatatatat gaacaaacca tcccaaaaag cttaacaagc 60
ttggtaatgc cgacacacaa agaatgggtg aaaatgtgat gggaagagta taaaatctga 120
gaattttact tttggaaccc gccaaattga tgttgttggt ttccaaggtt tgagaaaaag 180
tcgtctccag ctgctggctg tgaaggagtg aatccagacc tacccattcc aagaccagat 240
cctgaaccca tagctctccc catgtaaaat gaaggtggag ggcctttctc cctttcatcg 300
gatccatcac tcaatccacc cacaattcca acatccacaa gggatacttt tttaggagca 360
gttatattga gatcaatcag tccacggctt agtgaatcag cccagacgcc agacttcacc 420
tgaaaagcat cctttttagg ggaaacttta gagtctgcta aagatttacc agtcacatta 480
tcatgcttgc catcactgac aacattattg gagggctgtg aagacacaga atcagattga 540
gaagtaaaat caccaaaaat atcagatcca tcaaaattat taagaggaac agcagcaaag 600
ggatcgatag agctgttatt cataggaaca gacttctccg acttgttgtt tggctgcact 660
gccagttcag gaatggaaaa caagtcaact gtaggtgtaa ctgtaggaat ggctggttgt 720
gaagaaaata gatcaacctc agcctgtggt tgagaaatgg ctcctttatc cagatgaggt 780
tctgctgata caaatgatgc atctgcaaaa agatcaacct ctggtacatt actagttgct 840
ggcttttcta caggaacaga tgttggagca tccatgaggt caccgattaa atcttgtcca 900
aagagatcaa cctggttaga gtttccagct gaagttttgg tagaagttcc tcttggatca 960
aaatcatcca tgtcatcctc agtactgttt gcaggtacac ttgaactttg agaaggaact 1020
gaaccataat tatttgcatt agtagatgac tttgatactc tagatgactt gttctcctga 1080
cttttgctgg cagagcgagc agaacccttc ttgaaggagt tctcttgatt gtcactagcg 1140
gtggcatggt gtgattttcc ttggtaatct ttttcttctt cataggatcc tttgtctcta 1200
tagctatcat taaacctgtc accatctcta gatccaaagc ctccatattt accactgctt 1260
tgaaagctgc tgccactacc ataagaggag gctgacccag acttgtatgt gattccacta 1320
gaagaaactc caatgtactt gtcacggttt gcagcagctt tatttctgac ttcatgtatc 1380
ttatctttat cattcaaaag tgacacaatg ttttcagcct tcttccttac attgagtccc 1440
acatccttcc cactaggttc aacatattca aaactagaaa gtgcagagat ctgaaaagta 1500
tgttctataa tgtcatcaac tgcacgttca gatccatgtg ctaccaaata ttctatgaca 1560
gctaatgcct tgtatacata gcgccaatct tttccagttt cacccagtct tgtccaaagg 1620
acattcatga ctatttgaca ttcagtaaat tttttggtag cctgtgaaat ctctgccagc 1680
acagtaccat gaggacccca aggttcatta tcagtagcat ccagcacctt ctgttcgatt 1740
tccggaatct tgagcacctt cagattcacc tctctcttta tttctcgaac ggtttgatcg 1800
aagaccttca tgaaatccat ggcggtggat ctgatctgat ccgatctgaa gaggagaaag 1860
gttggaaagg ggttatgctt atgttaattg cagggaattc gatggctgta gttttcagta 1920
gaaatgattt gagatctaat acattcggat cgaatttttg ttttccttcc aaaaattttc 1980
ctcaaccctg cttcttttta ctcttcggaa ttaaatatgt ttttcattca cgagaatacc 2040
agtaaatttg c 2051
<210>114
<211>564
<212>PRT
<213>大豆
<400>114
Met Asp Phe Met Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Ile Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Asp Asn Glu Pro Trp Gly Pro His Gly Thr Val Leu
35 40 45
Ala Glu Ile Ser Gln Ala Thr Lys Lys Phe Thr Glu Cys Gln Ile Val
50 55 60
Met Asn Val Leu Trp Thr Arg Leu Gly Glu Thr Gly Lys Asp Trp Arg
65 70 75 80
Tyr Val Tyr Lys Ala Leu Ala Val Ile Glu Tyr Leu Val Ala His Gly
85 90 95
Ser Glu Arg Ala Val Asp Asp Ile Ile Glu His Thr Phe Gln Ile Ser
100 105 110
Ala Leu Ser Ser Phe Glu Tyr Val Glu Pro Ser Gly Lys Asp Val Gly
115 120 125
Leu Asn Val Arg Lys Lys Ala Glu Asn Ile Val Ser Leu Leu Asn Asp
130 135 140
Lys Asp Lys Ile His Glu Val Arg Asn Lys Ala Ala Ala Asn Arg Asp
145 150 155 160
Lys Tyr Ile Gly Val Ser Ser Ser Gly Ile Thr Tyr Lys Ser Gly Ser
165 170 175
Ala Ser Ser Tyr Gly Ser Gly Ser Ser Phe Gln Ser Ser Gly Lys Tyr
180 185 190
Gly G1y Phe Gly Ser Arg Asp Gly Asp Arg Phe Asn Asp Ser Tyr Arg
195 200 205
Asp Lys Gly Ser Tyr Glu Glu Glu Lys Asp Tyr Gln Gly Lys Ser His
210 215 220
His Ala Thr Ala Ser Asp Asn Gln Glu Asn Ser Phe Lys Lys Gly Ser
225 230 235 240
Ala Arg Ser Ala Ser Lys Ser Gln Glu Asn Lys Ser Ser Arg Val Ser
245 250 255
Lys Ser Ser Thr Asn Ala Asn Asn Tyr Gly Ser Val Pro Ser Gln Ser
260 265 270
Ser Ser Val Pro Ala Asn Ser Thr Glu Asp Asp Met Asp Asp Phe Asp
275 280 285
Pro Arg Gly Thr Ser Thr Lys Thr Ser Ala Gly Asn Ser Asn Gln Val
290 295 300
Asp Leu Phe Gly Gln Asp Leu Ile Gly Asp Leu Met Asp Ala Pro Thr
305 310 315 320
Ser Val Pro Val Glu Lys Pro Ala Thr Ser Asn Val Pro Glu Val Asp
325 330 335
Leu Phe Ala Asp Ala Ser Phe Val Ser Ala Glu Pro His Leu Asp Lys
340 345 350
Gly Ala Ile Ser Gln Pro Gln Ala Glu Val Asp Leu Phe Ser Ser Gln
355 360 365
Pro Ala Ile Pro Thr Val Thr Pro Thr Val Asp Leu Phe Ser Ile Pro
370 375 380
Glu Leu Ala Val Gln Pro Asn Asn Lys Ser Glu Lys Ser Val Pro Met
385 390 395 400
Asn Asn Ser Ser Ile Asp Pro Phe Ala Ala Val Pro Leu Asn Asn Phe
405 410 415
Asp Gly Ser Asp Ile Phe Gly Asp Phe Thr Ser Gln Ser Asp Ser Val
420 425 430
Ser Ser Gln Pro Ser Asn Asn Val Val Ser Asp Gly Lys His Asp Asn
435 440 445
Val Thr Gly Lys Ser Leu Ala Asp Ser Lys Val Ser Pro Lys Lys Asp
450 455 460
Ala Phe Gln Val Lys Ser Gly Val Trp Ala Asp Ser Leu Ser Arg Gly
465 470 475 480
Leu Ile Asp Leu Asn Ile Thr Ala Pro Lys Lys Val Ser Leu Val Asp
485 490 495
Val Gly Ile Val Gly Gly Leu Ser Asp Gly Ser Asp Glu Arg Glu Lys
500 505 510
Gly Pro Pro Pro Ser Phe Tyr Met Gly Arg Ala Met Gly Ser Gly Ser
515 520 525
Gly Leu Gly Met Gly Arg Ser Gly Phe Thr Pro Ser Gln Pro Ala Ala
530 535 540
Gly Asp Asp Phe Phe Ser Asn Leu Gly Asn Gln Gln His Gln Phe Gly
545 550 555 560
Gly Phe Gln Lys
<210>115
<211>2338
<212>DNA
<213>大麦(Hordeum vulgare)
<400>115
gaattcggcc gaggctctgc tcccctccct ccccgcacgc gcgcgccctc cgcccggcag 60
gccgacgacc tcgccgtcga ggtggccgcc gtccgtcgcg agggctcgcc gctcgccggg 120
atcgggggag ccgccgccgc caccgccgcg cctccatccc gtccagacgc agcctcctag 180
gccactcgct cgtctgccac taccagctca gcgcccgaca gcccagatcc ccccaccgta 240
tagagtcccc ggccagcctt cgacgccgcg ccggccatgg atttcatgaa ggccttcgat 300
cagaccgtaa gggagatcaa gagggaggtc aatctcaagg tgctcaaggt ccccgagctc 360
gaacagaagg tacttgacgc gacaagtgat gaaccttggg gtccccatgg ctctgctctt 420
tcagagctag ctcaggccac caagaagtac tctgagtgtc agatggtgat gggtgtcctc 480
tgggccaggt tgggtgagcg aggtgcaaac tggcgtcatg tgtataaggc actgacgatt 540
attgagtact tgatagcgaa tggttctgag cgggcagttg atgacattct cgatcattat 600
tcaaagatct cggttctttc gagttttgag tttgtggaac ctaatggaaa agactctgga 660
ataaatgtga ggaagaaagt ggaaactctg gtgggaatca tcaatgataa ggagagaata 720
aaggctgtga gagagaaagc tgccagtaac cgtgacaagt acgttgggtt atcatcaaca 780
gggtcaacat atagatcaag ctcagcctct gtaggtagca actacagttc aggtgaacgt 840
tatggcagtt ttggtggaac aagggaggct gattcgttca gtaacagtta cagggacaaa 900
gaacctgtta aaacctcttc aagtaatact ggcaggcgca aatctggcag caagataaga 960
aaggatgcgg atcatgatag aagctcatcg aagtccccat ctaacacaaa aggcaatgag 1020
gatgaattcg atgactttga tcctcgtgga tcttcttcaa atggtgcagc taatacaaag 1080
actgccgagg tggatcttct tggcccaaac ttgatggatg atttccttga cgagcctgca 1140
gccaccccag cagaaaaaag tgctttagaa cctcaggttg atctgtttgc tgatgcagat 1200
ttccaatcgg caaacccagg tgccgaaaca gctgcgcatc aggatgtcca ggaaactgtt 1260
gacctttttt cgggaaatgc aacctttgct tcagcctttc caccacagac tgggtttatc 1320
ccaccaccaa cttctgggac accttctaat gctaatactt ccgtgtccaa gaacacagta 1380
cccgaaccat ttgatccttt cggtgatatt cctttaagca gtttcggggg atctgaccca 1440
tttggtgact ttagctcctc tagtgcacca ccaccacctg tacacagctc cactggaaat 1500
atcagcacgt caagtcagaa ccttgaggca gcatcggact ttggtgcctt tgaatcaaac 1560
accgcggata cagctaaaga ccccttcgac ttttcttcca ctggcaattt tggcaaagca 1620
gatgtagcac ctttggcagc tcccaaaact gatgcgtctg attttggtgc cttcgttaca 1680
aacactgaag aagcagctaa agaccccttc aatctttctt caagtattaa tggaagggca 1740
gaccaaacac ctttggcagc tcccaagtcc aacaccaaga aagaaaattt tcaggtcaaa 1800
tctggcatat gggctgactc tttgagccgt ggattgattg atctgaacat aactggacca 1860
aagaaggtga acttagcaga tgttggggtc gtcggcggcc ttagtgatgg gttcgacgat 1920
aaggcacagc cctcatggaa catgggtatg ggtgcagggg tatctggcct aggaatgtct 1980
ggagttcctc cttctacgca aggcggtggc atcgagagct tggcaaacta caacaagtat 2040
cagtttggtg gcttcaagtg agctcatatg agaacatttt tagcacctgg tgtttgttac 2100
tttttgctgg actgcgcctt tgaatgctta ttgtcttatt cgttttgcca cctcgctttt 2160
gttgatccgg cacattcctt ataattgccg ctgtattcaa ttatcgtggt atttgtccag 2220
tgttatcatg cctgtgttgt gtttgttggg tttgcagaca acccttgtgt cacaccatga 2280
ttcgattcag tcttatggtt aatatgtgca ccttctgtaa acggcatgcc ttttgctt 2338
<210>116
<211>594
<212>PRT
<213>大麦
<400>116
Met Asp Phe Met Lys Ala Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Leu Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly SerAla Leu
35 40 45
Ser Glu Leu Ala Gln Ala Thr Lys Lys Tyr Ser Glu Cys Gln Met Val
50 55 60
Met Gly Val Leu Trp Ala Arg Leu Gly Glu Arg Gly Ala Asn Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Thr Ile Ile Glu Tyr Leu Ile Ala Asn Gly
85 90 95
Ser Glu Arg Ala Val Asp Asp Ile Leu Asp His Tyr Ser Lys Ile Ser
100 105 110
Val Leu Ser Ser Phe Glu Phe Val Glu Pro Asn Gly Lys Asp Ser Gly
115 120 125
Ile Asn Val Arg Lys Lys Val Glu Thr Leu Val Gly Ile Ile Asn Asp
130 135 140
Lys Glu Arg Ile Lys Ala Val Arg Glu Lys Ala Ala Ser Asn Arg Asp
145 150 155 160
Lys Tyr Val Gly Leu Ser Ser Thr Gly Ser Thr Tyr Arg Ser Ser Ser
165 170 175
Ala Ser Val Gly Ser Asn Tyr Ser Ser Gly Glu Arg Tyr Gly Ser Phe
180 185 190
Gly Gly Thr Arg Glu Ala Asp Ser Phe Ser Asn Ser Tyr Arg Asp Lys
195 200 205
Glu Pro Val Lys Thr Ser Ser Ser Asn Thr Gly Arg Arg Lys Ser Gly
210 215 220
Ser Lys Ile Arg Lys Asp Ala Asp His Asp Arg Ser Ser Ser Lys Ser
225 230 235 240
Pro Ser Asn Thr Lys Gly Asn Glu Asp Glu Phe Asp Asp Phe Asp Pro
245 250 255
Arg Gly Ser Ser Ser Asn Gly Ala Ala Asn Thr Lys Thr Ala Glu Val
260 265 270
Asp Leu Leu Gly Pro Asn Leu Met Asp Asp Phe Leu Asp Glu Pro Ala
275 280 285
Ala Thr Pro Ala Glu Lys Ser Ala Leu Glu Pro Gln Val Asp Leu Phe
290 295 300
Ala Asp Ala Asp Phe Gln Ser Ala Asn Pro Gly Ala Glu Thr Ala Ala
305 310 315 320
His Gln Asp Val Gln Glu Thr Val Asp Leu Phe Ser Gly Asn Ala Thr
325 330 335
Phe Ala Ser Ala Phe Pro Pro Gln Thr Gly Phe Ile Pro Pro Pro Thr
340 345 350
Ser Gly Thr Pro Ser Asn Ala Asn Thr Ser Val Ser Lys Asn Thr Val
355 360 365
Pro Glu Pro Phe Asp Pro Phe Gly Asp Ile Pro Leu Ser Ser Phe Gly
370 375 380
Gly Ser Asp Pro Phe Gly Asp Phe Ser Ser Ser Ser Ala Pro Pro Pro
385 390 395 400
Pro Val His Ser Ser Thr Gly Asn Ile Ser Thr Ser Ser Gln Ash Leu
405 410 415
Glu Ala Ala Ser Asp Phe Gly Ala Phe Glu Ser Asn Thr Ala Asp Thr
420 425 430
Ala Lys Asp Pro Phe Asp Phe Ser Ser Thr Gly Asn Phe Gly Lys Ala
435 440 445
Asp Val Ala Pro Leu Ala Ala Pro Lys Thr Asp Ala Ser Asp Phe Gly
450 455 460
Ala Phe Val Thr Asn Thr Glu Glu Ala Ala Lys Asp Pro Phe Asn Leu
465 470 475 480
Ser Ser Ser Ile Asn Gly Arg Ala Asp Gln Thr Pro Leu Ala Ala Pro
485 490 495
Lys Ser Asn Thr Lys Lys Glu Asn Phe Gln Val Lys Ser Gly Ile Trp
500 505 510
Ala Asp Ser Leu Ser Arg Gly Leu Ile Asp Leu Asn Ile Thr Gly Pro
515 520 525
Lys Lys Val Asn Leu Ala Asp Val Gly Val Val Gly Gly Leu Ser Asp
530 535 540
Gly Phe Asp Asp Lys Ala Gln Pro Ser Trp Asn Met Gly Met Gly Ala
545 550 555 560
Gly Val Ser Gly Leu Gly Met Ser Gly Val Pro Pro Ser Thr Gln Gly
565 570 575
Gly Gly Ile Glu Ser Leu Ala Asn Tyr Asn Lys Tyr Gln Phe Gly Gly
580 585 590
Phe Lys
<210>117
<211>701
<212>DNA
<213>蒺藜苜蓿
<400>117
aggctgaaat cttagatatc acagcaacag aacccatcga atttcgcctc aattaacaca 60
aagctacata acctttccct tcagatcaga tctacacaga tcaatcatca tggatttcat 120
gaaagtcttc gatcaaaccg ttcgagaaat taagagagag gtgaatctga aggtgcttaa 180
ggttcctgaa atcgaacaga aggtgttgga tgcaactgac gatgagcctt ggggtcctca 240
tggtactgtg ctggcagaga ttgcaactgc taccaaaaaa tttactgaat gtcagctggt 300
tatgaatgtt ctttggacaa gattggctga aactggaaag gattggcgat atgtatacaa 360
ggcattagct gttatagagt atttggtatc acatggatct gaacgtgcag ttgacgacat 420
tatagaacat acttttcaga tctctgcact ttctagcttt gaatatgttg agcctaatgg 480
gaaggatgtg ggactcaatg taaggaagaa ggcagaaaac attgttgccc ttttgaatga 540
tagagaaaag atacatgata ttagaaataa agctgctgca aaccgtgaca agtacgttgg 600
agtttcatct agtggaatga catacaaatc cgggaacacc tcatttagta gtgggagcag 660
taacaaatat gggaggtttt ggttcaagtg gtgataggtt t 701
<210>118
<211>194
<212>PRT
<213>蒺藜苜蓿
<400>118
Met Asp Phe Met Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Asp Asp Glu Pro Trp Gly Pro His Gly Thr Val Leu
35 40 45
Ala Glu Ile Ala Thr Ala Thr Lys Lys Phe Thr Glu Cys Gln Leu Val
50 55 60
Met Asn Val Leu Trp Thr Arg Leu Ala Glu Thr Gly Lys Asp Trp Arg
65 70 75 80
Tyr Val Tyr Lys Ala Leu Ala Val Ile Glu Tyr Leu Val Ser His Gly
85 90 95
Ser Glu Arg Ala Val Asp Asp Ile Ile Glu His Thr Phe Gln Ile Ser
100 105 110
Ala Leu Ser Ser Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Leu Asn Val Arg Lys Lys Ala Glu Asn Ile Val Ala Leu Leu Asn Asp
130 135 140
Arg Glu Lys Ile His Asp Ile Arg Asn Lys Ala Ala Ala Asn Arg Asp
145 150 155 160
Lys Tyr Val Gly Val Ser Ser Ser Gly Met Thr Tyr Lys Ser Gly Asn
165 170 175
Thr Ser Phe Ser Ser Gly Ser Ser Asn Lys Tyr Gly Arg Phe Trp Phe
180 185 190
Lys Trp
<210>119
<211>2032
<212>DNA
<213>蒺藜苜蓿
<220>
<221>misc_feature
<222>(18)..(18)
<223>n是a、c、g或t
<220>
<221>misc_feature
<222>(29)..(29)
<223>n是a、c、g或t
<400>119
accacgcggg ggccgcgntc ttgaactant gcagcaacag aacccatcga atttcgcctc 60
aattaacaca aagctacata acctttccct tcagatcaga tctacacaga tcaatcatca 120
tggatttcat gaaagtcttc gatcaaaccg ttcgagaaat taagagagag gtgaatctga 180
aggtgcttaa ggttcctgaa atcgaacaga aggtgttgga tgcaactgac gatgagcctt 240
ggggtcctca tggtactgtg ctggcagaga ttgcaactgc taccaaaaaa tttactgaat 300
gtcagctggt tatgaatgtt ctttggacaa gattggctga aactggaaag gattggcgat 360
atgtatacaa ggcattagct gttatagagt atttggtatc acatggatct gaacgtgcag 420
ttgacgacat tatagaacat acttttcaga tctctgcact ttctagcttt gaatatgttg 480
agcctaatgg gaaggatgtg ggactcaatg taaggaagaa ggcagaaaac attgttgccc 540
ttttgaatga tagagaaaag atacatgata ttagaaataa agctgctgca aaccgtgaca 600
agtacgttgg agtttcatct agtggaatga catacaaatc cgggacaacc tcatttagta 660
gtgggagcag taacaaatat ggaggttttg gttcaagtgg tgataggttt agtgatagct 720
acggagacaa ggggcgctat gatgaagcaa aaatagacaa agattactca ggaaaatcac 780
ggcatggtgt ctcaagtaaa aatgaagaga actccttcaa aaaaggttct gttcggtctg 840
tcagcaaaag tcaggagaac aaatcttcta gagcatcaaa gtcatctgct aatgcagttc 900
cttctcaaag tgcaagtgta cctactgagg acgactttga tgattttgat ccgagaggaa 960
catctagtaa gacttcagct ggaagctcaa accaggttga tctctttgga caagatttgc 1020
tcggtgactt catggatgct ccaacatctg tttctgtaga aaagccagca actagtaatg 1080
tgtcagatgt tgatctcttt gcggatgcat catttgtttc agcagcacca catgcggaca 1140
aaggagctag ttctcaacca caggatgaag tggatctttt ctcttcacaa ccagccattc 1200
cttcagttac accaacagtt gacttgtttt ccattccttc accagctgtg cagcctgaca 1260
gcaagtcgga aaactctgtt cctgtgaata acagcacttt tgatcccttt gcttctgttc 1320
cacttaataa ttttgaggga tctgatgttt ttggtgattt tacttctcaa tctgattctg 1380
tatcttctca gccctcaacg aatgctgcca ccgatggtag tacaagtggt aaatctgtaa 1440
cagactctaa tgtttcgcct aaaaaggatg cttttcaggt gaagtctggc atttgggctg 1500
attcactaag ccgtggattg attgacctca atatatctgc tcccaaaaaa agtatccctt 1560
gtcgatgttg gaattgtggg tggattaagt gatggattag atgaaaggga gaaaggccct 1620
ccaactacat tatacatggg gagagctatg ggtacaggat ccggtcttgg tatgtatggt 1680
ggcactcctt cacagtcaac agctggagat gacatatttt caaacttcgg tggccaaaac 1740
tatcaatttg gtgggttcca gaagtaaaaa aattcacaaa ttgtatactc attccatcac 1800
attttcactc attctttgtg tgtcggcttt atcaagattg ctgagctttt tggcatggtg 1860
gattttgtta atatatattt ctctctattt tgtatccaag aaaggtagga atagcaattt 1920
cctatcacta ttgtgattat attatcttaa attcatgcta ctgttgcatt ttaaatgttt 1980
gattacaaga tccagcaaac tgaagtaccc aaatagcttg tgatactgct tt 2032
<210>120
<211>490
<212>PRT
<213>蒺藜苜蓿
<400>120
Met Asp Phe Met Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Asp Asp Glu Pro Trp Gly Pro His Gly Thr Val Leu
35 40 45
Ala Glu Ile Ala Thr Ala Thr Lys Lys Phe Thr Glu Cys Gln Leu Val
50 55 60
Met Asn Val Leu Trp Thr Arg Leu Ala Glu Thr Gly Lys Asp Trp Arg
65 70 75 80
Tyr Val Tyr Lys Ala Leu Ala Val Ile Glu Tyr Leu Val Ser His Gly
85 90 95
Ser Glu Arg Ala Val Asp Asp Ile Ile Glu His Thr Phe Gln Ile Ser
100 105 110
Ala Leu Ser Ser Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Leu Asn Val Arg Lys Lys Ala Glu Asn Ile Val Ala Leu Leu Asn Asp
130 135 140
Arg Glu Lys Ile His Asp Ile Arg Asn Lys Ala Ala Ala Asn Arg Asp
145 150 155 160
Lys Tyr Val Gly Val Ser Ser Ser Gly Met Thr Tyr Lys Ser Gly Thr
165 170 175
Thr Ser Phe Ser Ser Gly Ser Ser Asn Lys Tyr Gly Gly Phe Gly Ser
180 185 190
Ser Gly Asp Arg Phe Ser Asp Ser Tyr Gly Asp Lys Gly Arg Tyr Asp
195 200 205
Glu Ala Lys Ile Asp Lys Asp Tyr Ser Gly Lys Ser Arg His Gly Val
210 215 220
Ser Ser Lys Asn Glu Glu Asn Ser Phe Lys Lys Gly Ser Val Arg Ser
225 230 235 240
Val Ser Lys Ser Gln Glu Asn Lys Ser Ser Arg Ala Ser Lys Ser Ser
245 250 255
Ala Asn Ala Val Pro Ser Gln Ser Ala Ser Val Pro Thr Glu Asp Asp
260 265 270
Phe Asp Asp Phe Asp Pro Arg Gly Thr Ser Ser Lys Thr Ser Ala Gly
275 280 285
Ser Ser Asn Gln Val Asp Leu Phe Gly Gln Asp Leu Leu Gly Asp Phe
290 295 300
Met Asp Ala Pro Thr Ser Val Ser Val Glu Lys Pro Ala Thr Ser Asn
305 310 315 320
Val Ser Asp Val Asp Leu Phe Ala Asp Ala Ser Phe Val Ser Ala Ala
325 330 335
Pro His Ala Asp Lys Gly Ala Ser Ser Gln Pro Gln Asp Glu Val Asp
340 345 350
Leu Phe Ser Ser Gln Pro Ala Ile Pro Ser Val Thr Pro Thr Val Asp
355 360 365
Leu Phe Ser Ile Pro Ser Pro Ala Val Gln Pro Asp Ser Lys Ser Glu
370 375 380
Asn Ser Val Pro Val Asn Asn Ser Thr Phe Asp Pro Phe Ala Ser Val
385 390 395 400
Pro Leu Asn Asn Phe Glu Gly Ser Asp Val Phe Gly Asp Phe Thr Ser
405 410 415
Gln Ser Asp Ser Val Ser Ser Gln Pro Ser Thr Asn Ala Ala Thr Asp
420 425 430
Gly Ser Thr Ser Gly Lys Ser Val Thr Asp Ser Asn Val Ser Pro Lys
435 440 445
Lys Asp Ala Phe Gln Val Lys Ser Gly Ile Trp Ala Asp Ser Leu Ser
450 455 460
Arg Gly Leu Ile Asp Leu Asn Ile Ser Ala Pro Lys Lys Ser Ile Pro
465 470 475 480
Cys Arg Cys Trp Asn Cys Gly Trp Ile Lys
485 490
<210>121
<211>983
<212>DNA
<213>展叶剑叶藓(Physcomitrella patents)
<400>121
aggcagtagt agtagtagtg gtagtagtac cggagtggag agggtttgcg acgcggagcg 60
ccatagccga gctcggctga gcagctatgg atctgaagaa ggtgttcgat cagactgtcc 120
gcgagctgaa gcgggaggtc aacaagaagg tgcttaaggt tcccgagatt gaagtgaaga 180
tactggaggc gaccagtaac gagccatggg gtccgcacgg aacgataatg ggtgacattg 240
cacaggctac tcggaaccac aacgactacc aaatgataat gaccgtcctt tacaaacgct 300
tgaatgacac tgggaggaat tggcgccatg tctacaaggc tttgacggtt ttggagtatt 360
tggttgcaaa cggctctgag agggtcattg atgaacttca agagcatact tatcagattc 420
agacgttgtg tgaatttcag tacttagagc aaagtggcaa ggatcaggga atcaatgtac 480
ggaagaaggc acaaactttg gttgccttaa tcaaggataa agataagatt cgtgaagtga 540
gaagcaaggc agcagccaac cgagacaaat atcgtggcgt ctccagcact ggaatgacac 600
atcgttctag ttcctattca agcaccggag gaggttatgg tgatagggac gatgaccggt 660
atggaggaag ccgtggagga agagatgaat atgattacag tgggagaggc ggcggccgag 720
atgctgatcg gtacagggat gatgatcctt acggaggagg tagagagcga agcaggaata 780
gtagttacga tggtcgtgat gcatctaagg aacgtgatgc ttatggatcg aagggaaatg 840
acagttatcc gtcaaaagga ggtgatcgag atggttatgc ctcaaaggga ggtgatcgtt 900
atgagaagta cgagagtgga tctgtaagtg accgcgaccg tgatagaggg tacgatgacg 960
acgacagtta ttcttccagg tga 983
<210>122
<211>298
<212>PRT
<213>展叶剑叶藓
<400>122
Met Asp Leu Lys Lys Val Phe Asp Gln Thr Val Arg Glu Leu Lys Arg
1 5 10 15
Glu Val Asn Lys Lys Val Leu Lys Val Pro Glu Ile Glu Val Lys Ile
20 25 30
Leu Glu Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Thr Ile Met
35 40 45
Gly Asp Ile Ala Gln Ala Thr Arg Asn His Asn Asp Tyr Gln Met Ile
50 55 60
Met Thr Val Leu Tyr Lys Arg Leu Asn Asp Thr Gly Arg Asn Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Thr Val Leu Glu Tyr Leu Val Ala Asn Gly
85 90 95
Ser Glu Arg Val Ile Asp Glu Leu Gln Glu His Thr Tyr Gln Ile Gln
100 105 110
Thr Leu Cys Glu Phe Gln Tyr Leu Glu Gln Ser Gly Lys Asp Gln Gly
115 120 125
Ile Asn Val Arg Lys Lys Ala Gln Thr Leu Val Ala Leu Ile Lys Asp
130 135 140
Lys Asp Lys Ile Arg Glu Val Arg Ser Lys Ala Ala Ala Asn Arg Asp
145 150 155 160
Lys Tyr Arg Gly Val Ser Ser Thr Gly Met Thr His Arg Ser Ser Ser
165 170 175
Tyr Ser Ser Thr Gly Gly Gly Tyr Gly Asp Arg Asp Asp Asp Arg Tyr
180 185 190
Gly Gly Ser Arg Gly Gly Arg Asp Glu Tyr Asp Tyr Ser Gly Arg Gly
195 200 205
Gly Gly Arg Asp Ala Asp Arg Tyr Arg Asp Asp Asp Pro Tyr Gly Gly
210 215 220
Gly Arg Glu Arg Ser Arg Asn Ser Ser Tyr Asp Gly Arg Asp Ala Ser
225 230 235 240
Lys Glu Arg Asp Ala Tyr Gly Ser Lys Gly Asn Asp Ser Tyr Pro Ser
245 250 255
Lys Gly Gly Asp Arg Asp Gly Tyr Ala Ser Lys Gly Gly Asp Arg Tyr
260 265 270
Glu Lys Tyr Glu Ser Gly Ser Val Ser Asp Arg Asp Arg Asp Arg Gly
275 280 285
Tyr Asp Asp Asp Asp Ser Tyr Ser Ser Arg
290 295
<210>123
<211>690
<212>DNA
<213>展叶剑叶藓
<400>123
atggatctaa agaaagtgtt ggaccaaacg gtccgagaat ttaagaggga agtgaacaag 60
aaagtcctca aggtgcctga aattgagctc aaagtttgcg aggccacgag caatgagccc 120
tggggaccgc atggagcaat catgggtgat atcgcacaag cgactcggaa cttccaagac 180
tatcacatga ttatgggggt tttgtggaag cgcttgcacg actctgggaa gaattggcga 240
cacgtctata agtcactagc agtgatggag tacctcattg ccaatggagc tgagcgagta 300
attgatgaac tgcgggagca ggcttaccaa atccaggttt tactggattt ccaacacatt 360
gagcccaacg gcaaagatca gggtatcaat gtgaggaaaa aggcagagag cctgttggcg 420
ctgatcaatg accctggcaa gattcgtgaa ctacagcaaa aggctgctgc taacagagat 480
aagtatcgag gcctctctaa tacggggatg agcttcaagc caagttcgta tttaagcaca 540
agcggtagct acagcgacaa agaaaacgag cggtatgggg gaggctcgag cgtgaatgca 600
caaaaggatg atgaagtcca caaggaagat cagcgagaat ccaacaggtc agagtactgg 660
caaaaagtga cgcttgcagg ctgtaattaa 690
<210>124
<211>229
<212>PRT
<213>展叶剑叶藓
<400>124
Met Asp Leu Lys Lys Val Leu Asp Gln Thr Val Arg Glu Phe Lys Arg
1 5 10 15
Glu Val Asn Lys Lys Val Leu Lys Val Pro Glu Ile Glu Leu Lys Val
20 25 30
Cys Glu Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ala Ile Met
35 40 45
Gly Asp Ile Ala Gln Ala Thr Arg Asn Phe Gln Asp Tyr His Met Ile
50 55 60
Met Gly Val Leu Trp Lys Arg Leu His Asp Ser Gly Lys Asn Trp Arg
65 70 75 80
His Val Tyr Lys Ser Leu Ala Val Met Glu Tyr Leu Ile Ala Asn Gly
85 90 95
Ala Glu Arg Val Ile Asp Glu Leu Arg Glu Gln Ala Tyr Gln Ile Gln
100 105 110
Val Leu Leu Asp Phe Gln His Ile Glu Pro Asn Gly Lys Asp Gln Gly
115 120 125
Ile Asn Val Arg Lys Lys Ala Glu Ser Leu Leu Ala Leu Ile Asn Asp
130 135 140
Pro Gly Lys Ile Arg Glu Leu Gln Gln Lys Ala Ala Ala Asn Arg Asp
145 150 155 160
Lys Tyr Arg Gly Leu Ser Asn Thr Gly Met Ser Phe Lys Pro Ser Ser
165 170 175
Tyr Leu Ser Thr Ser Gly Ser Tyr Ser Asp Lys Glu Asn Glu Arg Tyr
180 185 190
Gly Gly Gly Ser Ser Val Asn Ala Gln Lys Asp Asp Glu Val His Lys
195 200 205
Glu Asp Gln Arg Glu Ser Asn Arg Ser Glu Tyr Trp Gln Lys Val Thr
210 215 220
Leu Ala Gly Cys Asn
225
<210>125
<211>3285
<212>DNA
<213>展叶剑叶藓
<400>125
atgccacgtt ctgagctggg ggtgaaggca aaacgaggtc aagacccagg aattcctgca 60
gcatgggtcg tgccaggaat agaagcggcg aggagcgctg ttgcggtgca agctggccat 120
gaagaatcgc tccgccctct agaaccatca caactttgca gcttgcgacg ggatcgactg 180
ggagcggtaa tgactgattc atcgacctca ttattgtcgc aacgcatccg tcgcggtttt 240
ggcagcccat ccgcacgccg gacggaagga agcttgggct gcgtcttcgg ggatggaagt 300
cggtctcgac gcgctttgaa tccggtggaa ttttggggtt gtgcgggaag aagagcttcg 360
tctgtagctg agctcgggtg cgcagctatg gatctgaaga aagtgtttga tcagaccgtc 420
cgggagctga agcgagaggt caacaagaag gtgcttaagg ttcccgagat tgaagtgaag 480
gtggtcgttc tacatgtgga tgtgaataag cgtcgcaatg ctacgttggc agagctagca 540
tgcacctttt actgcactaa ttaccttctt ttctacaaga ctaaaaacga gacattcaag 600
gattctgcaa ggcagagaga gatagtgggc tgtacatctg ttcttttcga ggtccatgta 660
gaggggattt atatggtgtt ggaggcaacc agcaacgagc cttggggtcc tcatggatcg 720
atcatgggag acattgcgca ggcttctcga aattacaatg actaccaaat gataatggct 780
gtcctgtaca aacgcctaaa tgacactggg agaaattggc gccatgtgta caaggctttg 840
acagttttgg aattcttgat tgcaaatggc gcagagaggg tcatcgatga acttcaagag 900
catacttatc agatccagac attgtgcgat tttcaatacc tagagccaag tggcaaggat 960
cagggaatta atgtacggaa gaaggctcag actctggtag ctttgatcaa agataaagat 1020
agaatccgag aagtgagaag caaggcggct gccaatcgtg ataaatatcg tggagtctct 1080
agcaccggaa taactcaccg ctcaagttct tattcaagca ctaggggaag ttatggtcat 1140
agggatgatg agcggtatgg aggaagccgt gggggaagag acgaatatga ttacactggg 1200
aggggcagtg gccgagatgg tgatcggtat agggacgagg atccttacgg aagagatagg 1260
gagcgaagta gagatagtag ttacgatggg cgcgatgcat ctaaagagcg tgatgcttat 1320
gggtccaggg caggtgatag ttattcgtcg aaaggtggtg atcgcgatgg ttacggctca 1380
aaggcaggtg atcgttatga taagtacgag agcggatctg caagtgatcg cgaccgtgaa 1440
aggggttacg atgacgacga tagctactct tctaggaatg gcaagtcgaa aggcggatct 1500
acaccacctc ctgcttacga ggaagcggtt cagaggcttg cttccgacgt agatagtaac 1560
gagggccaag gtgtggctgc agcggtggca aaagctttca gcagtcagga agaggctact 1620
gtgctcaccg catcagagat cgtgccaaaa ccaagtgcaa attgctcttt tgatgcaagc 1680
caaacggcag acgatgattt tgatgatttt gaccctcgcg gaaccagcca ctcagcttct 1740
gcctctacag caagtcagcc aacggagaat ttctttccag aatcaggaaa tctatcttta 1800
ttccccgatt caacttctgc accccctgca cctcctgcac aagcacaact tcctccagca 1860
gcgcaaaaaa gtttaggtgg ccttgaagac ctattcggtg gtagttttga tgtagctcct 1920
gtttcaactt ctgttaccac tcccgcccca gcgcctccca atttcttctc gaacaacgcg 1980
gtgcaagcaa atgatttgtt tggagattca ttttctgtga ctccttctag tgcatctgat 2040
gggttcggag cttcagcttt tcaatccact ccagcttctg ctgcccctgc agtagcttcg 2100
gacccgtttg gtggatcgtc gttccaactg ggccctccac ccccaccccc tcctgtttat 2160
caaccgccag ctgcccctgc tagcaacttc ggaaataact ccttccagag tcatggtgca 2220
ccaggagtgc ccaatggttt gaatgcaaat atgttcgggc acccatcgac tccatttgac 2280
ccggctacta acaacaatgg attttctcat atatccgggc aaagctttgg caacaaccag 2340
agtttccctc gaaatcaatc gttcagcggc atgacccaag catccagtgt aaatcaatcc 2400
tattatggag cctcacagaa tatgggtggc ataaatagat ccgcttcagc gccacaagaa 2460
ttacagcact tggaacaacc accaaaacca cagcaaccag ctagaaagga attcggacca 2520
gtgaagtctg ccatttgggg agacacactt agcatgggcc ttgttgatct caacatcgca 2580
ggaccgaaag taaatcctct aaacgacctc ggaattcagt tgacagaccc tactctaaag 2640
aacgagcttt ggggtgccga caacccgaag caagcccaaa aaaagcctgc atcgtcatct 2700
gggatgggga tgggacagcc tatgggtaaa gctatgggat ccggcactgg ccttggtaga 2760
gcaggggcca gtggactacc tcctcctatg cctgcgacca caatggcaag accaggtatg 2820
ggcatgggtc ctcaacaggc tggtgccatg aacttcaaca tgggcatggg catgggcatg 2880
ggttctaata taggcatttc gccgggcatg agtatgggtc ctcagatggg aacgggtatg 2940
aaccagatgg gatcaatgtt acatatgggt atgggaggct acccaaacca acagcaacag 3000
tatggctata gtaaaacgct ttggcaaaat ttaacccctg attccatagt ccattgcggt 3060
ggacatgtat ttgtctttac aggaccagtt acgctttcat gcgtccgtgc ctacatgccg 3120
acattcttgg tcagatccag ctcttgtgtt gtggtggtct ttgttactat cactttcttt 3180
ccttaccact ctcactggtg gctttttgaa ttccctttcg tcagctcagt aaattctgat 3240
tatatcccag gcacaattcc tggtcattct ctttattggt tctga 3285
<210>126
<211>1094
<212>PRT
<213>展叶剑叶藓
<400>126
Met Pro Arg Ser Glu Leu Gly Val Lys Ala Lys Arg Gly Gln Asp Pro
1 5 10 15
Gly Ile Pro Ala Ala Trp Val Val Pro Gly Ile Glu Ala Ala Arg Ser
20 25 30
Ala Val Ala Val Gln Ala Gly His Glu Glu Ser Leu Arg Pro Leu Glu
35 40 45
Pro Ser Gln Leu Cys Ser Leu Arg Arg Asp Arg Leu Gly Ala Val Met
50 55 60
Thr Asp Ser Ser Thr Ser Leu Leu Ser Gln Arg Ile Arg Arg Gly Phe
65 70 75 80
Gly Ser Pro Ser Ala Arg Arg Thr Glu Gly Ser Leu Gly Cys Val Phe
85 90 95
Gly Asp Gly Ser Arg Ser Arg Arg Ala Leu Asn Pro Val Glu Phe Trp
100 105 110
Gly Cys Ala Gly Arg Arg Ala Ser Ser Val Ala Glu Leu Gly Cys Ala
115 120 125
Ala Met Asp Leu Lys Lys Val Phe Asp Gln Thr Val Arg Glu Leu Lys
130 135 140
Arg Glu Val Asn Lys Lys Val Leu Lys Val Pro Glu Ile Glu Val Lys
145 150 155 160
Val Val Val Leu His Val Asp Val Asn Lys Arg Arg Asn Ala Thr Leu
165 170 175
Ala Glu Leu Ala Cys Thr Phe Tyr Cys Thr Asn Tyr Leu Leu Phe Tyr
180 185 190
Lys Thr Lys Asn Glu Thr Phe Lys Asp Ser Ala Arg Gln Arg Glu Ile
195 200 205
Val Gly Cys Thr Ser Val Leu Phe Glu Val His Val Glu Gly Ile Tyr
210 215 220
Met Val Leu Glu Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser
225 230 235 240
Ile Met Gly Asp Ile Ala Gln Ala Ser Arg Asn Tyr Asn Asp Tyr Gln
245 250 255
Met Ile Met Ala Val Leu Tyr Lys Arg Leu Asn Asp Thr Gly Arg Asn
260 265 270
Trp Arg His Val Tyr Lys Ala Leu Thr Val Leu Glu Phe Leu Ile Ala
275 280 285
Asn Gly Ala Glu Arg Val Ile Asp Glu Leu Gln Glu His Thr Tyr Gln
290 295 300
Ile Gln Thr Leu Cys Asp Phe Gln Tyr Leu Glu Pro Ser Gly Lys Asp
305 310 315 320
Gln Gly Ile Asn Val Arg Lys Lys Ala Gln Thr Leu Val Ala Leu Ile
325 330 335
Lys Asp Lys Asp Arg Ile Arg Glu Val Arg Ser Lys Ala Ala Ala Asn
340 345 350
Arg Asp Lys Tyr Arg Gly Val Ser Ser Thr Gly Ile Thr His Arg Ser
355 360 365
Ser Ser Tyr Ser Ser Thr Arg Gly Ser Tyr Gly His Arg Asp Asp Glu
370 375 380
Arg Tyr Gly Gly Ser Arg Gly Gly Arg Asp Glu Tyr Asp Tyr Thr Gly
385 390 395 400
Arg Gly Ser Gly Arg Asp Gly Asp Arg Tyr Arg Asp Glu Asp Pro Tyr
405 410 415
Gly Arg Asp Arg Glu Arg Ser Arg Asp Ser Ser Tyr Asp Gly Arg Asp
420 425 430
Ala Ser Lys Glu Arg Asp Ala Tyr Gly Ser Arg Ala Gly Asp Ser Tyr
435 440 445
Ser Ser Lys Gly Gly Asp Arg Asp Gly Tyr Gly Ser Lys Ala Gly Asp
450 455 460
Arg Tyr Asp Lys Tyr Glu Ser Gly Ser Ala Ser Asp Arg Asp Arg Glu
465 470 475 480
Arg Gly Tyr Asp Asp Asp Asp Ser Tyr Ser Ser Arg Asn Gly Lys Ser
485 490 495
Lys Gly Gly Ser Thr Pro Pro Pro Ala Tyr Glu Glu Ala Val Gln Arg
500 505 510
Leu Ala Ser Asp Val Asp Ser Asn Glu Gly Gln Gly Val Ala Ala Ala
515 520 525
Val Ala Lys Ala Phe Ser Ser Gln Glu Glu Ala Thr Val Leu Thr Ala
530 535 540
Ser Glu Ile Val Pro Lys Pro Ser Ala Asn Cys Ser Phe Asp Ala Ser
545 550 555 560
Gln Thr Ala Asp Asp Asp Phe Asp Asp Phe Asp Pro Arg Gly Thr Ser
565 570 575
His Ser Ala Ser Ala Ser Thr Ala Ser Gln Pro Thr Glu Asn Phe Phe
580 585 590
Pro Glu Ser Gly Asn Leu Ser Leu Phe Pro Asp Ser Thr Ser Ala Pro
595 600 605
Pro Ala Pro Pro Ala Gln Ala Gln Leu Pro Pro Ala Ala Gln Lys Ser
610 615 620
Leu Gly Gly Leu Glu Asp Leu Phe Gly Gly Ser Phe Asp Val Ala Pro
625 630 635 640
Val Ser Thr Ser Val Thr Thr Pro Ala Pro Ala Pro Pro Asn Phe Phe
645 650 655
Ser Asn Asn Ala Val Gln Ala Asn Asp Leu Phe Gly Asp Ser Phe Ser
660 665 670
Val Thr Pro Ser Ser Ala Ser Asp Gly Phe Gly Ala Ser Ala Phe Gln
675 680 685
Ser Thr Pro Ala Ser Ala Ala Pro Ala Val Ala Ser Asp Pro Phe Gly
690 695 700
Gly Ser Ser Phe Gln Leu Gly Pro Pro Pro Pro Pro Pro Pro Val Tyr
705 710 715 720
Gln Pro Pro Ala Ala Pro Ala Ser Asn Phe Gly Asn Asn Ser Phe Gln
725 730 735
Ser His Gly Ala Pro Gly Val Pro Asn Gly Leu Asn Ala Asn Met Phe
740 745 750
Gly His Pro Ser Thr Pro Phe Asp Pro Ala Thr Asn Asn Asn Gly Phe
755 760 765
Ser His Ile Ser Gly Gln Ser Phe Gly Asn Asn Gln Ser Phe Pro Arg
770 775 780
Asn Gln Ser Phe Ser Gly Met Thr Gln Ala Ser Ser Val Asn Gln Ser
785 790 795 800
Tyr Tyr Gly Ala Ser Gln Asn Met Gly Gly Ile Asn Arg Ser Ala Ser
805 810 815
Ala Pro Gln Glu Leu Gln His Leu Glu Gln Pro Pro Lys Pro Gln Gln
820 825 830
Pro Ala Arg Lys Glu Phe Gly Pro Val Lys Ser Ala Ile Trp Gly Asp
835 840 845
Thr Leu Ser Met Gly Leu Val Asp Leu Asn Ile Ala Gly Pro Lys Val
850 855 860
Asn Pro Leu Asn Asp Leu Gly Ile Gln Leu Thr Asp Pro Thr Leu Lys
865 870 875 880
Asn Glu Leu Trp Gly Ala Asp Asn Pro Lys Gln Ala Gln Lys Lys Pro
885 890 895
Ala Ser Ser Ser Gly Met Gly Met Gly Gln Pro Met Gly Lys Ala Met
900 905 910
Gly Ser Gly Thr Gly Leu Gly Arg Ala Gly Ala Ser Gly Leu Pro Pro
915 920 925
Pro Met Pro Ala Thr Thr Met Ala Arg Pro Gly Met Gly Met Gly Pro
930 935 940
Gln Gln Ala Gly Ala Met Asn Phe Asn Met Gly Met Gly Met Gly Met
945 950 955 960
Gly Ser Asn Ile Gly Ile Ser Pro Gly Met Ser Met Gly Pro Gln Met
965 970 975
Gly Thr Gly Met Asn Gln Met Gly Ser Met Leu His Met Gly Met Gly
980 985 990
Gly Tyr Pro Asn Gln Gln Gln Gln Tyr Gly Tyr Ser Lys Thr Leu Trp
995 1000 1005
Gln Asn Leu Thr Pro Asp Ser Ile Val His Cys Gly Gly His Val
1010 1015 1020
Phe Val Phe Thr Gly Pro Val Thr Leu Ser Cys Val Arg Ala Tyr
1025 1030 1035
Met Pro Thr Phe Leu Val Arg Ser Ser Ser Cys Val Val Val Val
1040 1045 1050
Phe Val Thr Ile Thr Phe Phe Pro Tyr His Ser His Trp Trp Leu
l055 1060 1065
Phe Glu Phe Pro Phe Val Ser Ser Val Asn Ser Asp Tyr Ile Pro
1070 1075 1080
Gly Thr Ile Pro Gly His Ser Leu Tyr Trp Phe
1085 1090
<210>127
<211>2792
<212>DNA
<213>毛果杨
<400>127
cagtactgcc cttcaactcg tcctccgaca atgaagaagg cctttgatca aactgtcagg 60
gacatcaaga gagaggttaa taagaaagtg ctcaaagttc cttcgataga acagaaggtt 120
cttgatgcta ccagtaatga gccttggggt ccgcatggaa cacttcttgc agatattgca 180
caggcatcta gaaattatca tgaatatcag atgatcatgg cagttttatg gaaacggctt 240
aatgataccg gcaggaattg gcggcatgtc tacaaggcgt tgactgtttt ggaatatctg 300
gttgctcatg gatcagagcg tgtcatagat gagataaggg aacattcata tcaaatatcg 360
acattgtcaa atttccaata tattgactcc agtggaaagg accagggtaa taatgtcagg 420
aagaaatccc agagtcttgt ggccctggtg aatgataaag aaaggataat tgaagttaga 480
cagaaggctg ctgctaacag ggacaagttt cgcacatctc ctggtggaat gtatagacct 540
ggctcttatt caagtacagg ggggtatgat gacgatggtc gctataagga tgaggatcga 600
aatggatatg gttatggcaa agaaagagaa tacaattata gggatgatga ccggtatggt 660
aaatacgggg attcgtatgg tcgtgatgct gatcgttacg gtgaagaacg gtatggcaga 720
gatggatata gggatgatga ctatcaggga agaagtcgaa gcattgatgg ttatgggtca 780
agaagcagga gctccgacag agatagagat cgggcttttg atgacgatgg tcaatcctca 840
tctcgcggca gtggtgctag agctgatgat cgggctcagg atggaaggcg gcttgagcga 900
aaaccttccg agcaaaatat tggtggtcct cccagctatg aagaaactat aagtgaatcc 960
cgaagccctg ctcacagtga aaggaatggg gaaactttag cagcacctgc tcctggagct 1020
tctccacctc caagcaatcc tggacaagca aacacttttg atgtgaggtc tccatctcct 1080
gcagaccggg aaattgaggt ggctgatgaa tttgatccac gtggtcctgt ttcagcttcc 1140
cccacagcaa catctgttcc aactgcctct gctttcccta ccacctcaaa caatgctgaa 1200
atggatttac tcggctcttt atcagatgtt tttgctccaa atccattggc cattgtgcca 1260
gtcacttctg caacatcatc ctctgaagct gatgcccagg caaacttttc tggatccact 1320
tttgcttcaa cacaatcagc ctctaatatt atgaaccagg ctttcgaaga tccatttggt 1380
gataatcctt ttaaagctac tcctacagac aatttctcag cccaacagcc aactgcttcg 1440
gctgcccctt tccagcctac tatgaatcaa aatgctgaaa tgccccatgc agctgcacca 1500
ccaaatggtg atgcattctc tgacatgact tattcagcac ccaatgttca acctccttca 1560
acaaactcac acttttttcc tcaagaaatg tcaagttctc atccagaaac tgatattctg 1620
gcagacattc tctcatcatc cggtccttat gctgcagcct cacaggcagg tttttcattt 1680
ccttctggcc aacctccaca gttaggggct agcatgtacg ggaacttcaa tccaccagca 1740
gggaatatgg taccgccagc agttccacac atggctcctc agacccaaca attaagcagt 1800
gcaaacttcc ttccacaggg aggattccca gctactaacc cttccaacat ggctctacaa 1860
cctccagctg gaccagtggc acattttaac aatggaaact ttccacaaca aggttctgca 1920
gccccagttg tccctcaatt ttctcaccat acactgactg gatcaacccc acaatttaac 1980
agtggaaact ttcacccaca acgtggctct acagtcccag tttcttcaca atttacttac 2040
caaactccaa ccgcttcatc tcctcaacag aaagatgtcc tgggtaattt gttttctcaa 2100
ggatcgaact cttccatgtc atctcagaca gctcttccat cttcaacagg gtcgcttgct 2160
atagttcctc taccatccaa agacaaattt gagaccaagt caacagtgtg ggcagatact 2220
atgagtagag ggctggtcaa tttgaatatt tctggaccta aaaccaatcc attggctgac 2280
attggagttg attttgatgc ccttaatcgc aaggaaaaga gaatggaaaa acagcccatg 2340
actccagtta catctactgt caccatgggc aaagctatgg ggtctggtac tgggatgggc 2400
cgacctggtg caggtgttct cagcccacct ccaaacccta tgattggttc tggtatggga 2460
atgggtgtgg gtgcgggcgt gaatatgggc attggtatgg gtggtcgtcc tggttcaggc 2520
attggcatgg gaggttatgg aggagtgcac cagcccatgg gtgtgggtgt gggtgtggga 2580
atggggatga tgaacatggg aatgatgcca ggtggccaga tgcctcctgg atcagccatg 2640
cctggtggct ataaccccat gatgggttct ggtggttaca ctccccaaca acattatggc 2700
ggatatcgat gagatggatc aaccatactg aagggcaacg aatacctgtt gatttttgga 2760
ggtaaaaggg gatggcattg ctaaccccaa gt 2792
<210>128
<211>893
<212>PRT
<213>毛果杨
<400>128
Met Lys Lys Ala Phe Asp Gln Thr Val Arg Asp Ile Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Ser Ile Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Thr Leu Leu Ala Asp
35 40 45
Ile Ala Gln Ala Ser Arg Asn Tyr His Glu Tyr Gln Met Ile Met Ala
50 55 60
Val Leu Trp Lys Arg Leu Asn Asp Thr Gly Arg Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Leu Val Ala His Gly Ser Glu
85 90 95
Arg Val Ile Asp Glu Ile Arg Glu His Ser Tyr Gln Ile Ser Thr Leu
100 105 110
Ser Asn Phe Gln Tyr Ile Asp Ser Ser Gly Lys Asp Gln Gly Asn Asn
115 120 125
Val Arg Lys Lys Ser Gln Ser Leu Val Ala Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Ile Glu Val Arg Gln Lys Ala Ala Ala Asn Arg Asp Lys Phe
145 150 155 160
Arg Thr Ser Pro Gly Gly Met Tyr Arg Pro Gly Ser Tyr Ser Ser Thr
165 170 175
Gly Gly Tyr Asp Asp Asp Gly Arg Tyr Lys Asp Glu Asp Arg Asn Gly
180 185 190
Tyr Gly Tyr Gly Lys Glu Arg Glu Tyr Asn Tyr Arg Asp Asp Asp Arg
195 200 205
Tyr Gly Lys Tyr Gly Asp Ser Tyr Gly Arg Asp Ala Asp Arg Tyr Gly
210 215 220
Glu Glu Arg Tyr Gly Arg Asp Gly Tyr Arg Asp Asp Asp Tyr Gln Gly
225 230 235 240
Arg Ser Arg Ser Ile Asp Gly Tyr Gly Ser Arg Ser Arg Ser Ser Asp
245 250 255
Arg Asp Arg Asp Arg Ala Phe Asp Asp Asp Gly Gln Ser Ser Ser Arg
260 265 270
Gly Ser Gly Ala Arg Ala Asp Asp Arg Ala Gln Asp Gly Arg Arg Leu
275 280 285
Glu Arg Lys Pro Ser Glu Gln Asn Ile Gly Gly Pro Pro Ser Tyr Glu
290 295 300
Glu Thr Ile Ser Glu Ser Arg Ser Pro Ala His Ser Glu Arg Asn Gly
305 310 315 320
Glu Thr Leu Ala Ala Pro Ala Pro Gly Ala Ser Pro Pro Pro Ser Asn
325 330 335
Pro Gly Gln Ala Asn Thr Phe Asp Val Arg Ser Pro Ser Pro Ala Asp
340 345 350
Arg Glu Ile Glu Val Ala Asp Glu Phe Asp Pro Arg Gly Pro Val Ser
355 360 365
Ala Ser Pro Thr Ala Thr Ser Val Pro Thr Ala Ser Ala Phe Pro Thr
370 375 380
Thr Ser Asn Asn Ala Glu Met Asp Leu Leu Gly Ser Leu Ser Asp Val
385 390 395 400
Phe Ala Pro Asn Pro Leu Ala Ile Val Pro Val Thr Ser Ala Thr Ser
405 410 415
Ser Ser Glu Ala Asp Ala Gln Ala Asn Phe Ser Gly Ser Thr Phe Ala
420 425 430
Ser Thr Gln Ser Ala Ser Asn Ile Met Asn Gln Ala Phe Glu Asp Pro
435 440 445
Phe Gly Asp Asn Pro Phe Lys Ala Thr Pro Thr Asp Asn Phe Ser Ala
450 455 460
Gln Gln Pro Thr Ala Ser Ala Ala Pro Phe Gln Pro Thr Met Asn Gln
465 470 475 480
Asn Ala Glu Met Pro His Ala Ala Ala Pro Pro Asn Gly Asp Ala Phe
485 490 495
Ser Asp Met Thr Tyr Ser Ala Pro Asn Val Gln Pro Pro Ser Thr Asn
500 505 510
Ser His Phe Phe Pro Gln Glu Met Ser Ser Ser His Pro Glu Thr Asp
515 520 525
Ile Leu Ala Asp Ile Leu Ser Ser Ser Gly Pro Tyr Ala Ala Ala Ser
530 535 540
Gln Ala Gly Phe Ser Phe Pro Ser Gly Gln Pro Pro Gln Leu Gly Ala
545 550 555 560
Ser Met Tyr Gly Asn Phe Asn Pro Pro Ala Gly Asn Met Val Pro Pro
565 570 575
Ala Val Pro His Met Ala Pro Gln Thr Gln Gln Leu Ser Ser Ala Asn
580 585 590
Phe Leu Pro Gln Gly Gly Phe Pro Ala Thr Asn Pro Ser Asn Met Ala
595 600 605
Leu Gln Pro Pro Ala Gly Pro Val Ala His Phe Asn Asn Gly Asn Phe
610 615 620
Pro Gln Gln Gly Ser Ala Ala Pro Val Val Pro Gln Phe Ser His His
625 630 635 640
Thr Leu Thr Gly Ser Thr Pro Gln Phe Asn Ser Gly Asn Phe His Pro
645 650 655
Gln Arg Gly Ser Thr Val Pro Val Ser Ser Gln Phe Thr Tyr Gln Thr
660 665 670
Pro Thr Ala Ser Ser Pro Gln Gln Lys Asp Val Leu Gly Asn Leu Phe
675 680 685
Ser Gln Gly Ser Asn Ser Ser Met Ser Ser Gln Thr Ala Leu Pro Ser
690 695 700
Ser Thr Gly Ser Leu Ala Ile Val Pro Leu Pro Ser Lys Asp Lys Phe
705 710 715 720
Glu Thr Lys Ser Thr Val Trp Ala Asp Thr Met Ser Arg Gly Leu Val
725 730 735
Asn Leu Asn Ile Ser Gly Pro Lys Thr Asn Pro Leu Ala Asp Ile Gly
740 745 750
Val Asp Phe Asp Ala Leu Asn Arg Lys Glu Lys Arg Met Glu Lys Gln
755 760 765
Pro Met Thr Pro Val Thr Ser Thr Val Thr Met Gly Lys Ala Met Gly
770 775 780
Ser Gly Thr Gly Met Gly Arg Pro Gly Ala Gly Val Leu Ser Pro Pro
785 790 795 800
Pro Asn Pro Met Ile Gly Ser Gly Met Gly Met Gly Val Gly Ala Gly
805 810 815
Val Asn Met Gly Ile Gly Met Gly Gly Arg Pro Gly Ser Gly Ile Gly
820 825 830
Met Gly Gly Tyr Gly Gly Val His Gln Pro Met Gly Val Gly Val Gly
835 840 845
Val Gly Met Gly Met Met Asn Met Gly Met Met Pro Gly Gly Gln Met
850 855 860
Pro Pro Gly Ser Ala Met Pro Gly Gly Tyr Asn Pro Met Met Gly Ser
865 870 875 880
Gly Gly Tyr Thr Pro Gln Gln His Tyr Gly Gly Tyr Arg
885 890
<210>129
<211>2846
<212>DNA
<213>毛果杨
<400>129
cattgccgtt cttcaactat tctgctgata atgaagaagg tctttggtca aactgtcagg 60
gacttcaaga gagaggttaa taagaaagtt ctcaaagttc cttcgataga gcagaaggtt 120
cttgatgcta ccagcaatga accttggggt ccacatggat cactgcttgc agatattgcg 180
caggcatcaa gaaattatca tgaataccag atgatcatgg cagttctctg gaaacgaatt 240
aatgatactg ggaagaattg gcggcatgtc tacaaggctt tgactgtttt ggaatacctg 300
gttgctcatg gatcagagcg tgccatagat gagattaggg aacattctta tcaaataacg 360
acattgtcag atttccaata tattgattcc agcggaaagg accagggtaa caatgtcagg 420
aagaaatccc agagccttgt ggtcctggtg aatgataaag aaaggatagt tgaggctaga 480
cagaaggctg ctgctaacag ggacaagttt cgcaacgcat cacctggtgg aatgaataga 540
cctggttctt atgatgatga cggtcgctat ggaaacaggg atgaagatcg aaatggatat 600
ggttatggca aagaaagaga atacaattat agggatgatg aacggtatgg taaatatggg 660
gactcgtatg gtcgtgatgg agatcataat ggtgaagaac ggtatggtag ggatggatat 720
agggatgatg actatcaggg aagaagccga agcattgatg attacggttc aagaagcagg 780
agctctgata gagatagaga tcatgctttt gatgatgatg gtcaatcctc atctcgtggc 840
gctagagctg atgatcagtc tcatgacgga agcattgcta agcggcttga gcgaaaattt 900
tctgagcaaa atattagtgg tccacccagc tatgaagaag ccttaagtga atcccgaagc 960
cctgctcaca gtgaaaggaa tggagaagct ttagcagtac ctgctcctgt agcttcttct 1020
ccaccagctc ctagatcttt ctctccacct gcttttaatg ctgcttctcc acctccaagc 1080
aatcctggac aagaaaacac tttttttgcg acgcctgcat ctcctgcaga ccaggaagtt 1140
gtggtggctg atgaatttga tccacgtggt cctatttcag ctccccctac tgccacatct 1200
gttcaaactg cctctgcttt cactccgact tcgaacaatg ctgaaatgga cttacttggc 1260
tctttatcag atgtgtttac tccaaatcca ttagccatca tgccagttac ttctgcaaca 1320
actacctctg aagctgattc tcaaacaaac ttttcaggat ccatgtttgc tgcaacacaa 1380
tcaccatcaa atgttatgaa tcaggctttt gaagatccat ttggtgactc tcctttcaaa 1440
gctactccta cagatgcttt ctcagctcaa cagcccactg cttcttctgc ccctttccag 1500
cctaccatga atcaaaatac agaaatgccc aatgcagtag caccaccaaa tggtgacaca 1560
ttctctgcca tgacttattc agcacccaat gttcaacctc cttcaacaaa tccacacttt 1620
ttgcctcaag aaatgtcaag ttctcatcca gaaactgata ttctggcaga catcctccca 1680
ccatctggtc catctgctgt agcttcacag gcaggttttt cacttccatc tggccaacat 1740
ccacagccag gtgctagtgt gtatgggaac ttcaattcac caccagggaa tatggtattg 1800
ctggcagctc cacacatggc tcctcagggc cagcaattaa gcagtgcaaa cttctttaca 1860
cagggaggat ccccagctcc tatccattcc aacatgtctc tacaacctcc ggctgggcca 1920
gtggtgctgt ttaataatgg aaacttagtt ccacaacaag gttctacggc tccagttgtc 1980
tcacaatttt ctcatcatac accgactgga tcagcaccac aatataacag tggaaacttt 2040
ctcccacaac atggctctac tttcccagtc gcgtcacaat ttacttacca aactccacct 2100
gcttcatctc ctcaacataa tgatgtcctg ggtaatttgt tttcacaagg gccaaacact 2160
tccatggctt ctcagacagc tcttccatct tcaacagggt cgcttgctat agttcctcaa 2220
ccatccaagg acaaatttga gactaagtca acagtgtggg cagatactct gagtagaggg 2280
ctggtcaatt tgaatatttc tggacctaaa accaatccat tagctgacat tggagtcgat 2340
tttgatgccc ttaatcgcaa ggaaaagaga atggaaaaac agcccatgac tccagttgta 2400
tctactatca ccatgggcag agctatggga tctggtactg ggttaggccg agctggtgca 2460
ggtgttctca ggccacctcc aaaccctacg attggttctg gcatgggaat gggtgggggt 2520
gtgaatatgg gcatgggcat gggtatgggt ggtcctggtt caggcattgg catgggaggt 2580
tatggaggaa tgcaacaacc catgggtggt gtgggtatgg gagcagggat gaatgcgggc 2640
atgaatatgg gaatgatgca aggtggccag atgcctcctg gatcggccat gcctggtgga 2700
tataacccca tgatgggctc tggtggttac ccgtcccaac aaccctatgg tggtggatat 2760
cgatgagatg gagctgcctt gttgatgtgt tagtatacct gctgatttta caaggtaaaa 2820
gggatggcat cgcaaaccca gtgtta 2846
<210>130
<211>911
<212>PRT
<213>毛果杨
<400>130
Met Lys Lys Val Phe Gly Gln Thr Val Arg Asp Phe Lys Arg Glu Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Ser Ile Glu Gln Lys Val Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser Leu Leu Ala Asp
35 40 45
Ile Ala Gln Ala Ser Arg Asn Tyr His Glu Tyr Gln Met Ile Met Ala
50 55 60
Val Leu Trp Lys Arg Ile Asn Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Ala Leu Thr Val Leu Glu Tyr Leu Val Ala His Gly Ser Glu
85 90 95
Arg Ala Ile Asp Glu Ile Arg Glu His Ser Tyr Gln Ile Thr Thr Leu
100 105 110
Ser Asp Phe Gln Tyr Ile Asp Ser Ser Gly Lys Asp Gln Gly Asn Asn
115 120 125
Val Arg Lys Lys Ser Gln Ser Leu Val Val Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Val Glu Ala Arg Gln Lys Ala Ala Ala Asn Arg Asp Lys Phe
145 150 155 160
Arg Asn Ala Ser Pro Gly Gly Met Asn Arg Pro Gly Ser Tyr Asp Asp
165 170 175
Asp Gly Arg Tyr Gly Asn Arg Asp Glu Asp Arg Asn Gly Tyr Gly Tyr
180 185 190
Gly Lys Glu Arg Glu Tyr Asn Tyr Arg Asp Asp Glu Arg Tyr Gly Lys
195 200 205
Tyr Gly Asp Ser Tyr Gly Arg Asp Gly Asp His Asn Gly Glu Glu Arg
210 215 220
Tyr Gly Arg Asp Gly Tyr Arg Asp Asp Asp Tyr Gln Gly Arg Ser Arg
225 230 235 240
Ser Ile Asp Asp Tyr Gly Ser Arg Ser Arg Ser Ser Asp Arg Asp Arg
245 250 255
Asp His Ala Phe Asp Asp Asp Gly Gln Ser Ser Ser Arg Gly Ala Arg
260 265 270
Ala Asp Asp Gln Ser His Asp Gly Ser Ile Ala Lys Arg Leu Glu Arg
275 280 285
Lys Phe Ser Glu Gln Asn Ile Ser Gly Pro Pro Ser Tyr Glu Glu Ala
290 295 300
Leu Ser Glu Ser Arg Ser Pro Ala His Ser Glu Arg Asn Gly Glu Ala
305 310 315 320
Leu Ala Val Pro Ala Pro Val Ala Ser Ser Pro Pro Ala Pro Arg Ser
325 330 335
Phe Ser Pro Pro Ala Phe Asn Ala Ala Ser Pro Pro Pro Ser Asn Pro
340 345 350
Gly Gln Glu Asn Thr Phe Phe Ala Thr Pro Ala Ser Pro Ala Asp Gln
355 360 365
Glu Val Val Val Ala Asp Glu Phe Asp Pro Arg Gly Pro Ile Ser Ala
370 375 380
Pro Pro Thr Ala Thr Ser Val Gln Thr Ala Ser Ala Phe Thr Pro Thr
385 390 395 400
Ser Asn Asn Ala Glu Met Asp Leu Leu Gly Ser Leu Ser Asp Val Phe
405 410 415
Thr Pro Asn Pro Leu Ala Ile Met Pro Val Thr Ser Ala Thr Thr Thr
420 425 430
Ser Glu Ala Asp Ser Gln Thr Asn Phe Ser Gly Ser Met Phe Ala Ala
435 440 445
Thr Gln Ser Pro Ser Asn Val Met Asn Gln Ala Phe Glu Asp Pro Phe
450 455 460
Gly Asp Ser Pro Phe Lys Ala Thr Pro Thr Asp Ala Phe Ser Ala Gln
465 470 475 480
Gln Pro Thr Ala Ser Ser Ala Pro Phe Gln Pro Thr Met Asn Gln Asn
485 490 495
Thr Glu Met Pro Asn Ala Val Ala Pro Pro Asn Gly Asp Thr Phe Ser
500 505 510
Ala Met Thr Tyr Ser Ala Pro Asn Val Gln Pro Pro Ser Thr Asn Pro
515 520 525
His Phe Leu Pro Gln Glu Met Ser Ser Ser His Pro Glu Thr Asp Ile
530 535 540
Leu Ala Asp Ile Leu Pro Pro Ser Gly Pro Ser Ala Val Ala Ser Gln
545 550 555 560
Ala Gly Phe Ser Leu Pro Ser Gly Gln His Pro Gln Pro Gly Ala Ser
565 570 575
Val Tyr Gly Asn Phe Asn Ser Pro Pro Gly Asn Met Val Leu Leu Ala
580 585 590
Ala Pro His Met Ala Pro Gln Gly Gln Gln Leu Ser Ser Ala Asn Phe
595 600 605
Phe Thr Gln Gly Gly Ser Pro Ala Pro Ile His Ser Asn Met Ser Leu
610 615 620
Gln Pro Pro Ala Gly Pro Val Val Leu Phe Asn Asn Gly Asn Leu Val
625 630 635 640
Pro Gln Gln Gly Ser Thr Ala Pro Val Val Ser Gln Phe Ser His His
645 650 655
Thr Pro Thr Gly Ser Ala Pro Gln Tyr Asn Ser Gly Asn Phe Leu Pro
660 665 670
Gln His Gly Ser Thr Phe Pro Val Ala Ser Gln Phe Thr Tyr Gln Thr
675 680 685
Pro Pro Ala Ser Ser Pro Gln His Asn Asp Val Leu Gly Asn Leu Phe
690 695 700
Ser Gln Gly Pro Asn Thr Ser Met Ala Ser Gln Thr Ala Leu Pro Ser
705 710 715 720
Ser Thr Gly Ser Leu Ala Ile Val Pro Gln Pro Ser Lys Asp Lys Phe
725 730 735
Glu Thr Lys Ser Thr Val Trp Ala Asp Thr Leu Ser Arg Gly Leu Val
740 745 750
Asn Leu Asn Ile Ser Gly Pro Lys Thr Asn Pro Leu Ala Asp Ile Gly
755 760 765
Val Asp Phe Asp Ala Leu Asn Arg Lys Glu Lys Arg Met Glu Lys Gln
770 775 780
Pro Met Thr Pro Val Val Ser Thr Ile Thr Met Gly Arg Ala Met Gly
785 790 795 800
Ser Gly Thr Gly Leu Gly Arg Ala Gly Ala Gly Val Leu Arg Pro Pro
805 810 815
Pro Asn Pro Thr Ile Gly Ser Gly Met Gly Met Gly Gly Gly Val Asn
820 825 830
Met Gly Met Gly Met Gly Met Gly Gly Pro Gly Ser Gly Ile Gly Met
835 840 845
Gly Gly Tyr Gly Gly Met Gln Gln Pro Met Gly Gly Val Gly Met Gly
850 855 860
Ala Gly Met Asn Ala Gly Met Asn Met Gly Met Met Gln Gly Gly Gln
865 870 875 880
Met Pro Pro Gly Ser Ala Met Pro Gly Gly Tyr Asn Pro Met Met Gly
885 890 895
Ser Gly Gly Tyr Pro Ser Gln Gln Pro Tyr Gly Gly Gly Tyr Arg
900 905 910
<210>131
<211>1829
<212>DNA
<213>毛果杨
<400>131
aatcagacgc caccgatttg cagatccatc atggatttca tcaaagtctt tgatcaaaca 60
gtccgcgaaa taaagagaga ggtcaatctc aaggtcttga aggttcctga aatcgaacaa 120
aaggtgttgg atgccactga tgatgcacct tggggtccac atggctctgc aatggctgag 180
atcgcgcaag ctactaaaaa attcacggaa tgtcaaatga tcatgaatgt cctgtggacg 240
aggttaggtg agacgggcaa ggactggcgt cttgtttaca aggcattggc tgtcatagaa 300
tatttggtgg cacatggatc tgatcgtgct gttgatgaca taatagaaca cacattccag 360
atatcatccc tcacaagttt cgaatatgtt gagccaaacg gaaaggactc aggacttaat 420
gtgaggaaga aggctgaaac tattgtatcc cttttgaaca ataaggataa aatatacgaa 480
gtcaggagta aggctgctac taaccgtgat aagtatgttg gtctttcatc tagtggaata 540
tcttataagt caagcacagc ctcatttgga agcagcagca gcagcttcca aagtagagat 600
cggtatggag gtggcttaag tggtacggga gatgaagata gctatgatag ttttaaacaa 660
gaaaagagtc gaaaggattc ctacacgaag tcacatcgaa ggggtataag tgacaatcaa 720
gacagcacct tgaaaaaggg ttctgcaggc tttggaagca aggatcaaga taatttgtct 780
tctcgtgcat caaggtcatc tgctaaccta aatgcctctg ataagaaaaa ctcagtttca 840
agtaattatg atgacgatga ttttgatgat tttgatcccc gaggaacctc cagcactaag 900
tctgcttctc aaacctcaaa ccaagttgat ttgtttggag aaagtttgat tggtgatctc 960
atggatgcac ctccatctgt tcctacagaa gctttagtca tgaatgggaa ttctgcagag 1020
gttgatctgt ttgctgatgc agcttttgta tcagcacctc cccaagctgg aaaggaagca 1080
agttcacaga ctcagacaaa ggttgatctg ttcacttccc aacctgccaa cccttctgca 1140
gttccttcta catttgactt cttttctagt gatgaaccag tcgcacaacc cgggatcaag 1200
actccaaaat ctgatcctcc aaatactaat gtctttgacc catttgctgc agttccaatg 1260
aacaattttg atggatctga tctttttggt gtattcactt ctagttctaa tccagcttct 1320
tcagaaccaa ctcaaagtcc tattaaagat ggcagccttg acaatttgga tgggaagtct 1380
ttggcaagct ccaatcctcc acagaaggag ggtttccagg tgaagtctgg aatatgggca 1440
gattcgctta gccgtggact gattgatctt aatatatctg ctcgctcagt cagaattgtc 1500
ttgccacttg aactccttgc agccaagaag gtgtctcttg tagatgttgg tgtcgtgggt 1560
gatcttacta atggattaga tgaaagggaa aagggacctc caacttcatt ctatatggga 1620
acagcgatgg gcatgggatc tggccttggt aaatccgggt taacagcttc acagtcacaa 1680
gcaactggcg gagatgattt cttctcaagc cttggtggcc aacaatacca ctttggtaat 1740
tttaagtaaa ttctgtcaag tggcctttat cgcccattat gcccgcatat tcttttgccc 1800
gtttggtaaa gatatttcaa gtgctgcct 1829
<210>132
<211>572
<212>PRT
<213>毛果杨
<400>132
Met Asp Phe Ile Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Asp Asp Ala Pro Trp Gly Pro His Gly Ser Ala Met
35 40 45
Ala Glu Ile Ala Gln Ala Thr Lys Lys Phe Thr Glu Cys Gln Met Ile
50 55 60
Met Asn Val Leu Trp Thr Arg Leu Gly Glu Thr Gly Lys Asp Trp Arg
65 70 75 80
Leu Val Tyr Lys Ala Leu Ala Val Ile Glu Tyr Leu Val Ala His Gly
85 90 95
Ser Asp Arg Ala Val Asp Asp Ile Ile Glu His Thr Phe Gln Ile Ser
100 105 110
Ser Leu Thr Ser Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Ser Gly
115 120 125
Leu Asn Val Arg Lys Lys Ala Glu Thr Ile Val Ser Leu Leu Asn Asn
130 135 140
Lys Asp Lys Ile Tyr Glu Val Arg Ser Lys Ala Ala Thr Asn Arg Asp
145 150 155 160
Lys Tyr Val Gly Leu Ser Ser Ser Gly Ile Ser Tyr Lys Ser Ser Thr
165 170 175
Ala Ser Phe Gly Ser Ser Ser Ser Ser Phe Gln Ser Arg Asp Arg Tyr
180 185 190
Gly Gly Gly Leu Ser Gly Thr Gly Asp Glu Asp Ser Tyr Asp Ser Phe
195 200 205
Lys Gln Glu Lys Ser Arg Lys Asp Ser Tyr Thr Lys Ser His Arg Arg
210 215 220
Gly Ile Ser Asp Asn Gln Asp Ser Thr Leu Lys Lys Gly Ser Ala Gly
225 230 235 240
Phe Gly Ser Lys Asp Gln Asp Asn Leu Ser Ser Arg Ala Ser Arg Ser
245 250 255
Ser Ala Asn Leu Asn Ala Ser Asp Lys Lys Asn Ser Val Ser Ser Asn
260 265 270
Tyr Asp Asp Asp Asp Phe Asp Asp Phe Asp Pro Arg Gly Thr Ser Ser
275 280 285
Thr Lys Ser Ala Ser Gln Thr Ser Asn Gln Val Asp Leu Phe Gly Glu
290 295 300
Ser Leu Ile Gly Asp Leu Met Asp Ala Pro Pro Ser Val Pro Thr Glu
305 310 315 320
Ala Leu Val Met Asn Gly Asn Ser Ala Glu Val Asp Leu Phe Ala Asp
325 330 335
Ala Ala Phe Val Ser Ala Pro Pro Gln Ala Gly Lys Glu Ala Ser Ser
340 345 350
Gln Thr Gln Thr Lys Val Asp Leu Phe Thr Ser Gln Pro Ala Asn Pro
355 360 365
Ser Ala Val Pro Ser Thr Phe Asp Phe Phe Ser Ser Asp Glu Pro Val
370 375 380
Ala Gln Pro Gly Ile Lys Thr Pro Lys Ser Asp Pro Pro Asn Thr Asn
385 390 395 400
Val Phe Asp Pro Phe Ala Ala Val Pro Met Asn Asn Phe Asp Gly Ser
405 410 415
Asp Leu Phe Gly Val Phe Thr Ser Ser Ser Asn Pro Ala Ser Ser Glu
420 425 430
Pro Thr Gln Ser Pro Ile Lys Asp Gly Ser Leu Asp Asn Leu Asp Gly
435 440 445
Lys Ser Leu Ala Ser Ser Asn Pro Pro Gln Lys Glu Gly Phe Gln Val
450 455 460
Lys Ser Gly Ile Trp Ala Asp Ser Leu Ser Arg Gly Leu Ile Asp Leu
465 470 475 480
Asn Ile Ser Ala Arg Ser Val Arg Ile Val Leu Pro Leu Glu Leu Leu
485 490 495
Ala Ala Lys Lys Val Ser Leu Val Asp Val Gly Val Val Gly Asp Leu
500 505 510
Thr Asn Gly Leu Asp Glu Arg Glu Lys Gly Pro Pro Thr Ser Phe Tyr
515 520 525
Met Gly Thr Ala Met Gly Met Gly Ser Gly Leu Gly Lys Ser Gly Leu
530 535 540
Thr Ala Ser Gln Ser Gln Ala Thr Gly Gly Asp Asp Phe Phe Ser Ser
545 550 555 560
Leu Gly Gly Gln Gln Tyr His Phe Gly Asn Phe Lys
565 570
<210>133
<211>802
<212>DNA
<213>番茄
<220>
<221>misc_feature
<222>(786)..(786)
<223>n是a、c、g或t
<400>133
aaaaaaeaga gtagctattt ctctcttctc ttggcaaagc aagagttgag agagatctca 60
tcggctttat actttttgaa gagatctcat ccttttttcc tttcagtttc agatctctat 120
atctttttcc agaattttga atcaggtttc gcttattggg tagattcagc catggatttc 180
atgaaggtct tcgatcaaac ggttcgcgaa ataaagaggg aggtgaattt gaaagtattg 240
aaagtccctg agatcgagca gaaggtattg gatgctactg atgatgagcc ctggggccct 300
catggtactg ctttggctga gatagcacag gctacaaaaa aattctccga gtgtcagatg 360
gtcatgaatg tgctctggac aagattgact gaaactggaa agaactggcg atatgtctat 420
aagtcattgg ctgttataga gtacttggtg gcgcatggag ctgaacgtgc aattgatgac 480
atcatagaac atacctatca gatctcttct ctcacaactt tcgagtatgt tgagccaaat 540
ggtaaggatg tgggaattaa tgtgaggaag aaggcggaaa atgttgtggc attattaaat 600
aacaaagaga agatacaaga ggttaggagt aaagctactg caaatcgtga gaagtacgtt 660
ggactatcat cttccggaat aacatataag gcaagctctg cctcttttag tagcagcaga 720
agcggcttac agagtagtga acgggtatgg aggttttgga aataaaagcg atggtgattc 780
atttanggat agttataatg aa 802
<210>134
<211>197
<212>PRT
<213>番茄
<400>134
Met Asp Phe Met Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Asp Asp Glu Pro Trp Gly Pro His Gly Thr Ala Leu
35 40 45
Ala Glu Ile Ala Gln Ala Thr Lys Lys Phe Ser Glu Cys Gln Met Val
50 55 60
Met Asn Val Leu Trp Thr Arg Leu Thr Glu Thr Gly Lys Asn Trp Arg
65 70 75 80
Tyr Val Tyr Lys Ser Leu Ala Val Ile Glu Tyr Leu Val Ala His Gly
85 90 95
Ala Glu Arg Ala Ile Asp Asp Ile Ile Glu His Thr Tyr Gln Ile Ser
100 105 110
Ser Leu Thr Thr Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Ile Asn Val Arg Lys Lys Ala Glu Asn Val Val Ala Leu Leu Asn Asn
130 135 140
Lys Glu Lys Ile Gln Glu Val Arg Ser Lys Ala Thr Ala Asn Arg Glu
145 150 155 160
Lys Tyr Val Gly Leu Ser Ser Ser Gly Ile Thr Tyr Lys Ala Ser Ser
165 170 175
Ala Ser Phe Ser Ser Ser Arg Ser Gly Leu Gln Ser Ser Glu Arg Val
180 185 190
Trp Arg Phe Trp Lys
195
<210>135
<21l>1754
<212>DNA
<213>普通小麦(Triticum aestivum)
<400>135
ccacgcgtcc gcggacgcgt gggcggacgc gtgggcggac gcgtgggcgg acgcgtgggc 60
gcgcgcgccc tccgcccggc aggccgtcga cctcgccgtc cgtcgaggtg gccgccggcc 120
gtcgcgagga atcgccgctc gccgggatcg ggggagccgc cgccgcgtct ccatcccatc 180
cagacacagc ctcctaggcc actcgctcag cgctcgagag cccagatccc ccgctatata 240
gagtccccgt cgacccttcg ccgccgcgca ggccatggat ttcatgaagg ccttcgatca 300
gaccgtaagg gagataaaga gggaggtcaa tctcaaggtg ctcaaggtcc ccgagctcga 360
acagaaggtc cttgacgcga caagtgatga accttggggt ccacacgggt ctgctctttc 420
agagctagct caggccacca agaaatactc tgagtgtcag atggtgatgg gtgtcctctg 480
ggccaggctg ggtgagcgag atgcaaactg gcgtcatgtg tataaggcac tgacgattat 540
tgagtacttg atagcgaatg gttctgaccg ggcagttgat gacattctcg atcattattc 600
aaagatctcg gttctttcga gttttgagtt tgtggaacct aatggaaaag actctggaat 660
aaatgtgagg aagaaagtgg aaactctggt ggggatcatc aatgataagg agagaataaa 720
ggctgtgaga gagaaagcgg ctagtaaccg tgacaagtac gttgggttat catcaactgg 780
gtcatataga tcaagctcag cctctggagg tagcaactac agttcaggtg aacgctatgg 840
cagttttggt ggcacaaggg aggctgattc gttcagtaac agttacaggg acaaagaacc 900
tgttaaaacc tcttcaagta atactggcag gcgcagatct ggcagcaaga taagaaagga 960
cgcggatcat gatagaagct cctcgaagtc cccatctaac acaaaaggca atgaggatga 1020
atttgatgac tttgatcctc gtggatcttc aaatggtgca gctaatacaa agactgccga 1080
ggtggatctt ctcggcccaa acttgatgga tgatttcctt gacgagcctg cagccactcc 1140
agcagcaaaa agtgctgtag aacctcaggt tgatctgttt gctgatgcag atttccaatc 1200
ggcaatccca ggtgccgaaa caactgcgca tcaggatgtc caggaaactg ttgacctttt 1260
ttcgggaaat gctacctttg cttcagcatt tccaccacag actgggttta tccaaccacc 1320
aacttctggg acaccttcta atgctaatac ttccgtgtcc aagaacacag tacctgaacc 1380
atttgatcct ttcggtgata ttcctttaag cagttttggg ggatctgacc catttggtga 1440
cttcagctca tctagtgcac caccaccacc tgtacacagc tctactggaa atatcagcac 1500
gtcgagtcag aaccttgagg cagcatcgga ctttggtgcc tttgaatcaa acaccgtgga 1560
tacagctaaa gaccccttcg acttttcttc cactggcaat tttggcaaag cagatgtagc 1620
acctttggca gctcccaaaa ctgatgcgtc tgactttggt gccttcgttg caaacactgt 1680
ataagcaact aaagacccct gtgatctttc ttcaagtatt aatggaaggg cagaccaaac 1740
acctttggct gctc 1754
<210>136
<211>469
<212>PRT
<213>普通小麦
<400>136
Met Asp Phe Met Lys Ala Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Leu Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly Ser rAla Leu
35 40 45
Ser Glu Leu Ala Gln Ala Thr Lys Lys Tyr Ser Glu Cys Gln Met Val
50 55 60
Met Gly Val Leu Trp Ala Arg Leu Gly Glu Arg Asp Ala Asn Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Thr Ile Ile Glu Tyr Leu Ile Ala Asn Gly
85 90 95
Ser Asp Arg Ala Val Asp Asp Ile Leu Asp His Tyr Ser Lys Ile Ser
100 105 110
Val Leu Ser Ser Phe Glu Phe Val Glu Pro Asn Gly Lys Asp Ser Gly
115 120 125
Ile Asn Val Arg Lys Lys Val Glu Thr Leu Val Gly Ile Ile Asn Asp
130 135 140
Lys Glu Arg Ile Lys Ala Val Arg Glu Lys Ala Ala Ser Asn Arg Asp
145 150 155 160
Lys Tyr Val Gly Leu Ser Ser Thr Gly Ser Tyr Arg Ser Ser Ser Ala
165 170 175
Ser Gly Gly Ser Asn Tyr Ser Ser Gly Glu Arg Tyr Gly Ser Phe Gly
180 185 190
Gly Thr Arg Glu Ala Asp Ser Phe Ser Asn Ser Tyr Arg Asp Lys Glu
195 200 205
Pro Val Lys Thr Ser Ser Ser Asn Thr Gly Arg Arg Arg Ser Gly Ser
210 215 220
Lys Ile Arg Lys Asp Ala Asp His Asp Arg Ser Ser Ser Lys Ser Pro
225 230 235 240
Ser Asn Thr Lys Gly Asn Glu Asp Glu Phe Asp Asp Phe Asp Pro Arg
245 250 255
Gly Ser Ser Asn Gly Ala Ala Asn Thr Lys Thr Ala Glu Val Asp Leu
260 265 270
Leu Gly Pro Asn Leu Met Asp Asp Phe Leu Asp Glu Pro Ala Ala Thr
275 280 285
Pro Ala Ala Lys Ser Ala Val Glu Pro Gln Val Asp Leu Phe Ala Asp
290 295 300
Ala Asp Phe Gln Ser Ala Ile Pro Gly Ala Glu Thr Thr Ala His Gln
305 310 315 320
Asp Val Gln Glu Thr Val Asp Leu Phe Ser Gly Asn Ala Thr Phe Ala
325 330 335
Ser Ala Phe Pro Pro Gln Thr Gly Phe Ile Gln Pro Pro Thr Ser Gly
340 345 350
Thr Pro Ser Asn Ala Asn Thr Ser Val Ser Lys Asn Thr Val Pro Glu
355 360 365
Pro Phe Asp Pro Phe Gly Asp Ile Pro Leu Ser Ser Phe Gly Gly Ser
370 375 380
Asp Pro Phe Gly Asp Phe Ser Ser Ser Ser Ala Pro Pro Pro Pro Val
385 390 395 400
His Ser Ser Thr Gly Asn Ile Ser Thr Ser Ser Gln Asn Leu Glu Ala
405 410 415
Ala Ser Asp Phe Gly Ala Phe Glu Ser Asn Thr Val Asp Thr Ala Lys
420 425 430
Asp Pro Phe Asp Phe Ser Ser Thr Gly Asn Phe Gly Lys Ala Asp Val
435 440 445
Ala Pro Leu Ala Ala Pro Lys Thr Asp Ala Ser Asp Phe Gly Ala Phe
450 455 460
Val Ala Asn Thr Val
465
<210>137
<211>947
<212>DNA
<213>普通小麦
<400>137
tcccgggtcg accagcggca gtccgatccg ccgccgctcg tcccgcctcc tcacccaggt 60
ctcctaccga ggaggaggag gaggaggagc aggaggaggg gaggctgaca cccgggtgcg 120
cgcagggggc tagctagatt cggaagggcg ccatgaagaa agtgttcgac cagacggtcc 180
gggacctgaa acgcggggtg aataaaaagg tcctcaaggt gcctggcacg gaacagaaga 240
ttctcgacgc cacaagcaat gagccgtggg gcccccatgg atccctcctg gcggagatcg 300
cccaagcaac acataactat catgagtatc agatgatact gaatatcgtg tggaagaggg 360
tcagcgatac tggtaaaaat tggcggcatg tgtacaaggg attgattgtc ctagattacc 420
tggtagcaca tggaaccgag cgagttattg atgacataag ggagcattct tatcagatat 480
cggcactagc tgacttccag tatatcgatt ctagtgggag agatcaaggt agcaatgtac 540
gacggaaatc ccagagcctc gttagtttag ttaatgataa ggaaaggata ctggaagtta 600
ggcagaaagc acttgctacc agagacaagt atcggagtgc atttgccaca agtggaccac 660
acaggagtcc gggtggatat gacaatgacc gatcgctacg aaggaggtag atatgataac 720
aggaatggct atgggaggga acgagacgga tatagagatg atgacagata cagtggcgct 780
ggagatacac ccaataggga tggagatcaa tattctaggg attctaatga acgcaacagg 840
gaagatgaat acaacagagg aagtaatagc aaccctgagt atgcagaagg atcaggccgt 900
aggagctacg gggatgagga ggcttattca tcccgtggtc gtggcag 947
<210>138
<211>185
<212>PRT
<213>普通小麦
<400>138
Met Lys Lys Val Phe Asp Gln Thr Val Arg Asp Leu Lys Arg Gly Val
1 5 10 15
Asn Lys Lys Val Leu Lys Val Pro Gly Thr Glu Gln Lys Ile Leu Asp
20 25 30
Ala Thr Ser Asn Glu Pro Trp Gly Pro His Gly Ser Leu Leu Ala Glu
35 40 45
Ile Ala Gln Ala Thr His Asn Tyr His Glu Tyr Gln Met Ile Leu Asn
50 55 60
Ile Val Trp Lys Arg Val Ser Asp Thr Gly Lys Asn Trp Arg His Val
65 70 75 80
Tyr Lys Gly Leu Ile Val Leu Asp Tyr Leu Val Ala His Gly Thr Glu
85 90 95
Arg Val Ile Asp Asp Ile Arg Glu His Ser Tyr Gln Ile Ser Ala Leu
100 105 110
Ala Asp Phe Gln Tyr Ile Asp Ser Ser Gly Arg Asp Gln Gly Ser Asn
115 120 125
Val Arg Arg Lys Ser Gln Ser Leu Val Ser Leu Val Asn Asp Lys Glu
130 135 140
Arg Ile Leu Glu Val Arg Gln Lys Ala Leu Ala Thr Arg Asp Lys Tyr
145 150 155 160
Arg Ser Ala Phe Ala Thr Ser Gly Pro His Arg Ser Pro Gly Gly Tyr
165 170 175
Asp Asn Asp Arg Ser Leu Arg Arg Arg
180 185
<210>139
<211>1683
<212>DNA
<213>拟南芥
<400>139
atggatttca tgaaggtctt cgatcaaact gttcgagaga taaaaaggga ggtgaatctg 60
aaagttttga aggtccctga gatggagcaa aaggtattgg atgctacaga taatgagcct 120
tggggtcccc atggtactgc attggcagag atagctcagg ccacgaagaa attctcggag 180
tgccagatgg ttatgagtgt tttgtggact agactaagtg agacaggaaa agattggcga 240
tatgtctaca aggcattggc tgttattgat tatctgattt cgaatggatc agaacgagcg 300
gttgatgaga ttattgagca tacttaccaa atatcttcac tcacaagttt tgagtatgtt 360
gaaccaaatg ggaaagatgt gggaatcaac gtgagaaaga aggcggaaaa catagtcgct 420
cttttaaata ataaagagaa aatctcagaa atcagagaca aagcagtagc aaatcgtaac 480
aagtatgttg gcctctcatc aacgggaata acatataaat ctggttcctc agcttcattt 540
ggtggtagtt ttcaaagtgg ttcaagcaat tttgacagct acaaggatag agattccaga 600
gaagacaaga atgactacga gtcttttcaa aagtcaagac gtggtgttaa aactgaggag 660
caaagctaca cttctaagaa gagtttttca cgctatggca gcacggacca tgacaatcta 720
tccagtggta aaaagtcacc cgattctgcc aaacataggt catatgtatc tgcagctcct 780
tcaaacaacg atgatgattt tgatgatttt gatccacggg gaacttccag taataagcca 840
tccacaggca gtgccaacca agtggacctt tttggaggag atctgattgg tgacttcttg 900
gattctgggc caacagaaac atcttccacc aacaacaacg agaactttca agaggcggac 960
ttgtttgctg atgcagcttt tgtatcagcc tcggctcagg gagctgaatt tgggtcacag 1020
acacagaaac aagttgatct tttctctgcg tctgaacctt ctgtcacagt ttcttcagct 1080
cctccgacgg ttgatctctt tgcatcttct gaatcagttg taagcccaga agccaagata 1140
tcaatacctg agtctatggc tactcccaac attgttgacc catttgctgc agttcctatg 1200
gataattttg atggatcaga tccttttggt gccttcactt cccactcggc ttcagtttct 1260
acaggtccac aagctccaag tgtacatggg agtgcaacaa acaccacaag cccgctgtct 1320
ttcgcagact cgaagccaca acatttgcaa aagaaagatc ctttccaggt gaaatcagga 1380
atttgggcag attcgctgag ccgtggactg attgatctca atataactgc tcccaaaaaa 1440
gcttctctag ctgatgtggg tgttgtgggg gatctgagca atgaagatgg gaacaaagca 1500
tctgctgcat cgtactactc aggctggtcc atgggtgcag gatccgggct tggtaaaact 1560
ggcctataca gtacacagca gcagcagcaa caacaacaac aacaacaagc acctgatatc 1620
tcagacgatt tcttctccag cctcagtaac caaaggtacc aatctggtgg ctttaagcag 1680
tga 1683
<210>140
<211>559
<212>PRT
<213>拟南芥
<400>140
Met Asp Phe Met Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Met Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Asp Asn Glu Pro Trp Gly Pro His Gly Thr Ala Leu
35 40 45
Ala Glu Ile Ala Gln Ala Thr Lys Lys Phe Ser Glu Cys Gln Met Val
50 55 60
Met Ser Val Leu Trp Thr Arg Leu Ser Glu Thr Gly Lys Asp Trp Arg
65 70 75 80
Tyr Val Tyr Lys Ala Leu Ala Val Ile Asp Tyr Leu Ile Ser Asn Gly
85 90 95
Ser Glu Arg Ala Val Asp Glu Ile Ile Glu His Thr Tyr Gln Ile Ser
100 105 110
Ser Leu Thr Ser Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Ile Asn Val Arg Lys Lys Ala Glu Asn Ile Val Ala Leu Leu Asn Asn
130 135 140
Lys Glu Lys Ile Ser Glu Ile Arg Asp Lys Ala Val Ala Asn Arg Asn
145 150 155 160
Lys Tyr Val Gly Leu Ser Ser Thr Gly Ile Thr Tyr Lys Ser Gly Ser
165 170 175
Ser Ala Ser Phe Gly Gly Ser Phe Gln Ser Gly Ser Ser Asn Phe Asp
180 185 190
Ser Tyr Lys Asp Arg Asp Ser Arg Glu Asp Lys Asn Asp Tyr Glu Ser
195 200 205
Phe Gln Lys Ser Arg Arg Gly Val Lys Thr Glu Glu Gln Ser Tyr Thr
210 215 220
Ser Lys Lys Ser Phe Ser Arg Tyr Gly Ser Thr Asp His Asp Asn Leu
225 230 235 240
Ser Ser Gly Lys Lys Ser Pro Asp Ser Ala Lys His Arg Ser Tyr Val
245 250 255
Ser Ala Ala Pro Ser Asn Asn Asp Asp Asp Phe Asp Asp Phe Asp Pro
260 265 270
Arg Gly Thr Ser Ser Asn Lys Pro Ser Thr Gly Ser Ala Asn Gln Val
275 280 285
Asp Leu Phe Gly Gly Asp Leu Ile Gly Asp Phe Leu Asp Ser Gly Pro
290 295 300
Thr Glu Thr Ser Ser Thr Asn Asn Asn Glu Asn Phe Gln Glu Ala Asp
305 310 315 320
Leu Phe Ala Asp Ala Ala Phe Val Ser Ala Ser Ala Gln Gly Ala Glu
325 330 335
Phe Gly Ser Gln Thr Gln Lys Gln Val Asp Leu Phe Ser Ala Ser Glu
340 345 350
Pro Ser Val Thr Val Ser Ser Ala Pro Pro Thr Val Asp Leu Phe Ala
355 360 365
Ser Ser Glu Ser Val Val Ser Pro Glu Ala Lys Ile Ser Ile Pro Glu
370 375 380
Ser Met Ala Thr Pro Asn Ile Val Asp Pro Phe Ala Ala Val Pro Met
385 390 395 400
Asp Asn Phe Asp Gly Ser Asp Pro Phe Gly Ala Phe Thr Ser His Ser
405 410 415
Ala Ser Val Ser Thr Gly Pro Gln Ala Pro Ser Val His Gly Ser Ala
420 425 430
Thr Asn Thr Thr Ser Pro Leu Ser Phe Ala Asp Ser Lys Pro Gln His
435 440 445
Leu Gln Lys Lys Asp Pro Phe Gln Val Lys Ser Gly Ile Trp Ala Asp
450 455 460
Ser Leu Ser Arg Gly Leu Ile Asp Leu Asn Ile Thr Ala Pro Lys Lys
465 470 475 480
Ala Ser Leu Ala Asp Val Gly Val Val Gly Asp Leu Ser Asn Glu Asp
485 490 495
Gly Asn Lys Ala Ser Ala Ala Ser Tyr Tyr Ser Gly Trp Ser Met Gly
500 505 510
Ala Gly Ser Gly Leu Gly Lys Thr Gly Leu Tyr Ser Thr Gln Gln Gln
515 520 525
Gln Gln Gln Gln Gln Gln Gln Gln Ala Pro Asp Ile Ser Asp Asp Phe
530 535 540
Phe Ser Ser Leu Ser Asn Gln Arg Tyr Gln Ser Gly Gly Phe Lys
545 550 555
<210>141
<211>2663
<212>DNA
<213>玉蜀黍(Zea mays)
<400>141
tcctctctgc tcacgagtaa agcctgccgc tagcccgcgc tctggacgtc gcctcctccc 60
ccaccccacc ggctgtcgcc gcccccgccg acgttacctc cttacccgcc gcaccccccc 120
ccccccctgc ccccccgtcc ttgccttggc ggatctgcct gcctgtgccg cctggcgctg 180
cctcttatcc cgccggccgc cgctgcctca cgttagctcc tcccccatcg tatccggacc 240
tccgcgccgc ccccacgcct ccctgacttg gtggatctgt ccaccatcca ccggaaacag 300
ttggtagccg cctaggtacc cacctacccc ctaggcgagg cggtaccccg tcgcccagcg 360
cataagcgcg cccaggcgag cgcggaatag ctccttttcg aatacactcg agcgaagctt 420
agatctgatc tcaaggaacc tgcagcttgc tgcagccact cctcgccggc gatggatttc 480
atgaaggttt tcgatcagac tgtgcgtgag ataaagaggg aggtcaatct gaaggtactc 540
aaggtccccg agatcgagca gaaggtactt gatgcaacaa gtgatgaacc gtggggccct 600
catggaagtg ctctttcaga acttgcacac gctaccaaga agttcgctga gtgccagatg 660
gtaatgaatg tcctatggac taggttgtct gagagaggcg cgaattggcg tcatgtgtat 720
aaggcattaa ctattatcga gtactttata gccaatggtt ctgagagggc agttgatgac 780
attcttgatc attattctaa gatctcggtt ctttcgagtt ttgagtatgt tgagcctaat 840
ggtaaagacg ttggaataaa tgtgaggaaa aaagtagaaa ccattgtggg catcataaat 900
gacaaggaga aaataaaggc cgtgagagat aaagcagcca gtaatcgtga taagtacatt 960
ggactatcat ccacaggcat aacatataaa tcaagctcag cctcatttgg tagcaactac 1020
agttcaggcg aacgttatgg gagtttcagt ggtacaaagg aagctgactc atacggtgat 1080
agttacaggg acaaagaacc tgttaaaact tccacaagca atagcggcag tcggaaatct 1140
ggcagcaagc tgaggaagga tgcgaagcct gataggagga atgaggatta tagttcaccc 1200
agctctttga gacccccatc taatacaaat aacactgagg acgactttga tgactttgat 1260
cctcgtggat cagatggcaa aacaactgct aagtcaaatg aggtggatct gtttagccca 1320
aacttgatgg acgattttat tgatgcatct gcagcaactc cagcaacaga caatgctgca 1380
gaaccacagg ttgatctgtt tgctgatgca gattttcaat cggcaaccgc aagtacagat 1440
acagctgcaa atacagatgt caaggtcaat gttgaccttt ttgcggaaaa ggcatctttt 1500
cctgcagcat ttccaccaca ggctgggttt attccaccac caagtgccgg gacatcttct 1560
gaagtaaata cttccgtatc caagaaggca gctccggaac cttttgatcc ttttggcgct 1620
attcctttga acagttttga tggacctgac ccatttggtg gcttcagctc aaatgctgga 1680
tcatctaccg cagcagcacc cacccacggt tcaactggaa atatcagcac atcgaaccag 1740
aaccttcatg ctgcaaccga ctttgctgcc tttgtatcaa acaatgaggg agcagcgaaa 1800
gacccattcg atctttcttc gagtgccaat gtcggaaaaa cacctttggc agctcccaag 1860
atcgacgcat ctgatttcgg tgcatttgta tctagcaccg aggaagcagc caaggaccct 1920
ctcgatcttt cttcgggtag caaccttgga aggacaggcc aagcacccgc ggcagcctcc 1980
aaggctaacg caaagaaaga caattttcag gtcaagtccg gcatatgggc agactcgctg 2040
agccggggat tgattgatct gaacataact gcaccgaaga aggtgaacct agctgatatt 2100
ggcatcgtcg gtggccttgg cgatgggtcc gatgagaagg ccctgccctc ttggaccatg 2160
ggcgccgtat ccggcctagg aatgtctggt attccaccgt caacacaaca agctggtggc 2220
atcgagagct tggccaacta caacaagcat catttcggct tcaaataggc ctcgatcttt 2280
catactggaa aatacccgtc atctgcggtt tcctcctctg tcggcctgct tcttacatgt 2340
gctgccctat tgatttaatc accttttgtt ttgttttgtt ttttgttttg gtgattacat 2400
tacatggtat cgaccaatct tggccccgtc ttgtcacgcg tgtatgttat ttgtcgggtt 2460
tgtgggtaag catgcaacta catacacatc acaccccctg tgttccagct cgatgatagg 2520
tggtatgttg gccatgcagt ttgtgtaaat tccgtggcct gccttctcta cttcacacag 2580
tactctacgc agcgatttgc agtgcttgag tttatattca ataataagag aacctatgcc 2640
ctttatgtct cgaaaaaaaa aaa 2663
<210>142
<211>598
<212>PRT
<213>玉蜀黍
<400>142
Met Asp Phe Met Lys Val Phe Asp Gln Thr Val Arg Glu Ile Lys Arg
1 5 10 15
Glu Val Asn Leu Lys Val Leu Lys Val Pro Glu Ile Glu Gln Lys Val
20 25 30
Leu Asp Ala Thr Ser Asp Glu Pro Trp Gly Pro His Gly Ser Ala Leu
35 40 45
Ser Glu Leu Ala His Ala Thr Lys Lys Phe Ala Glu Cys Gln Met Val
50 55 60
Met Asn Val Leu Trp Thr Arg Leu Ser Glu Arg Gly Ala Asn Trp Arg
65 70 75 80
His Val Tyr Lys Ala Leu Thr Ile Ile Glu Tyr Phe Ile Ala Asn Gly
85 90 95
Ser Glu Arg Ala Val Asp Asp Ile Leu Asp His Tyr Ser Lys Ile Ser
100 105 110
Val Leu Ser Ser Phe Glu Tyr Val Glu Pro Asn Gly Lys Asp Val Gly
115 120 125
Ile Asn Val Arg Lys Lys Val Glu Thr Ile Val Gly Ile Ile Asn Asp
130 135 140
Lys Glu Lys Ile Lys Ala Val Arg Asp Lys Ala Ala Ser Asn Arg Asp
145 150 155 160
Lys Tyr Ile Gly Leu Ser Ser Thr Gly Ile Thr Tyr Lys Ser Ser Ser
165 170 175
Ala Ser Phe Gly Ser Asn Tyr Ser Ser Gly Glu Arg Tyr Gly Ser Phe
180 185 190
Ser Gly Thr Lys Glu Ala Asp Ser Tyr Gly Asp Ser Tyr Arg Asp Lys
195 200 205
Glu Pro Val Lys Thr Ser Thr Ser Asn Ser Gly Ser Arg Lys Ser Gly
210 215 220
Ser Lys Leu Arg Lys Asp Ala Lys Pro Asp Arg Arg Asn Glu Asp Tyr
225 230 235 240
Ser Ser Pro Ser Ser Leu Arg Pro Pro Ser Asn Thr Asn Asn Thr Glu
245 250 255
Asp Asp Phe Asp Asp Phe Asp Pro Arg Gly Ser Asp Gly Lys Thr Thr
260 265 270
Ala Lys Ser Asn Glu Val Asp Leu Phe Ser Pro Asn Leu Met Asp Asp
275 280 285
Phe Ile Asp Ala Ser Ala Ala Thr Pro Ala Thr Asp Asn Ala Ala Glu
290 295 300
Pro Gln Val Asp Leu Phe Ala Asp Ala Asp Phe Gln Ser Ala Thr Ala
305 310 315 320
Ser Thr Asp Thr Ala Ala Asn Thr Asp Val Lys Val Asn Val Asp Leu
325 330 335
Phe Ala Glu Lys Ala Ser Phe Pro Ala Ala Phe Pro Pro Gln Ala Gly
340 345 350
Phe Ile Pro Pro Pro Ser Ala Gly Thr Ser Ser Glu Val Asn Thr Ser
355 360 365
Val Ser Lys Lys Ala Ala Pro Glu Pro Phe Asp Pro Phe Gly Ala Ile
370 375 380
Pro Leu Asn Ser Phe Asp Gly Pro Asp Pro Phe Gly Gly Phe Ser Ser
385 390 395 400
Asn Ala Gly Ser Ser Thr Ala Ala Ala Pro Thr His Gly Ser Thr Gly
405 410 415
Asn Ile Ser Thr Ser Asn Gln Asn Leu His Ala Ala Thr Asp Phe Ala
420 425 430
Ala Phe Val Ser Asn Asn Glu Gly Ala Ala Lys Asp Pro Phe Asp Leu
435 440 445
Ser Ser Ser Ala Asn Val Gly Lys Thr Pro Leu Ala Ala Pro Lys Ile
450 455 460
Asp Ala Ser Asp Phe Gly Ala Phe Val Ser Ser Thr Glu Glu Ala Ala
465 470 475 480
Lys Asp Pro Leu Asp Leu Ser Ser Gly Ser Asn Leu Gly Arg Thr Gly
485 490 495
Gln Ala Pro Ala Ala Ala Ser Lys Ala Asn Ala Lys Lys Asp Asn Phe
500 505 510
Gln Val Lys Ser Gly Ile Trp Ala Asp Ser Leu Ser Arg Gly Leu Ile
515 520 525
Asp Leu Asn Ile Thr Ala Pro Lys Lys Val Asn Leu Ala Asp Ile Gly
530 535 540
Ile Val Gly Gly Leu Gly Asp Gly Ser Asp Glu Lys Ala Leu Pro Ser
545 550 555 560
Trp Thr Met Gly Ala Val Ser Gly Leu Gly Met Ser Gly Ile Pro Pro
565 570 575
Ser Thr Gln Gln Ala Gly Gly Ile Glu Ser Leu Ala Asn Tyr Asn Lys
580 585 590
His His Phe Gly Phe Lys
595
<210>143
<211>921
<212>DNA
<213>聚球藻属物种(Synechococcus sp.)
<400>143
atggaatcgc gtttgaaacc aggtttgatt gtcctctgtg ggccaacggc ggcaggaaaa 60
tcgagtttgg cgatcgcgat cgcccagcgt ttgggcagcc caattttgag tgctgattcg 120
cggctggttt atcgcgactt taatattggc accgccaagc ccacccctgc cgagcagcag 180
caggtgcccc actatttaat ggatctctgt gatccccgcc aagtttttac cgttggggat 240
tatcaagatt gtgcagtgcc actgattcag caactccagg aaaaaggaat gctgcccttg 300
ttggtagggg gtacaggtct ctacatcaaa gcgatcgtca acggtttacg ctttccccgc 360
attgcaccgc agcctaagct gcgatcgcaa cttcaagcgt taggtcagcc actctgtcat 420
gccctattac agcgggttga tccggtggct ggcgaccgaa ttcatgtcag tgatcgcgtt 480
cggaccttgc gagccttaga agtcttctat gtcagtggcg atcgcctgac ggatctgcag 540
caggagcaac cgccgagcta tccaattctg cagattggct tggatagcga tcgcttagaa 600
gccaggattc agcagcgcac ccagcagatg ttaacgtcgg gcttcgttga ggaagtgcaa 660
ggattgtgcg atcgctacgg tagcgatctg ccgctgctca ataccctggg ctatcgccag 720
gtttgtgctt ttctacaggg aagtttgagt cggtcagagt tgccagagca aatcgtcctg 780
cagacgcgcc agtatgccaa acagcagcgc acttggttcc gggccgattc atcgattcag 840
tggattgatg ccgaagccag caatcgcctg gagcgggcgc tagacctgat tgagcgcttc 900
cgaaagagtg agggcgtttg a 921
<210>144
<211>306
<212>PRT
<213>聚球藻属物种
<400>144
Met Glu Ser Arg Leu Lys Pro Gly Leu Ile Val Leu Cys Gly Pro Thr
1 5 10 15
Ala Ala Gly Lys Ser Ser Leu Ala Ile Ala Ile Ala Gln Arg Leu Gly
20 25 30
Ser Pro Ile Leu Ser Ala Asp Ser Arg Leu Val Tyr Arg Asp Phe Asn
35 40 45
Ile Gly Thr Ala Lys Pro Thr Pro Ala Glu Gln Gln Gln Val Pro His
50 55 60
Tyr Leu Met Asp Leu Cys Asp Pro Arg Gln Val Phe Thr Val Gly Asp
65 70 75 80
Tyr Gln Asp Cys Ala Val Pro Leu Ile Gln Gln Leu Gln Glu Lys Gly
85 90 95
Met Leu Pro Leu Leu Val Gly Gly Thr Gly Leu Tyr Ile Lys Ala Ile
100 105 110
Val Asn Gly Leu Arg Phe Pro Arg Ile Ala Pro Gln Pro Lys Leu Arg
115 120 125
Ser Gln Leu Gln Ala Leu Gly Gln Pro Leu Cys His Ala Leu Leu Gln
130 135 140
Arg Val Asp Pro Val Ala Gly Asp Arg Ile His Val Ser Asp Arg Val
145 150 155 160
Arg Thr Leu Arg Ala Leu Glu Val Phe Tyr Val Ser Gly Asp Arg Leu
165 170 175
Thr Asp Leu Gln Gln Glu Gln Pro Pro Ser Tyr Pro Ile Leu Gln Ile
180 185 190
Gly Leu Asp Ser Asp Arg Leu Glu Ala Arg Ile Gln Gln Arg Thr Gln
195 200 205
Gln Met Leu Thr Ser Gly Phe Val Glu Glu Val Gln Gly Leu Cys Asp
210 215 220
Arg Tyr Gly Ser Asp Leu Pro Leu Leu Asn Thr Leu Gly Tyr Arg Gln
225 230 235 240
Val Cys Ala Phe Leu Gln Gly Ser Leu Ser Arg Ser Glu Leu Pro Glu
245 250 255
Gln Ile Val Leu Gln Thr Arg Gln Tyr Ala Lys Gln Gln Arg Thr Trp
260 265 270
Phe Arg Ala Asp Ser Ser Ile Gln Trp Ile Asp Ala Glu Ala Ser Asn
275 280 285
Arg Leu Glu Arg Ala Leu Asp Leu Ile Glu Arg Phe Arg Lys Ser Glu
290 295 300
Gly Val
305
<210>145
<211>909
<212>DNA
<213>Acaryochloris marina
<400>145
atgacgccag ggttgatcgt aatttgtggg ccaacggcta ccggaaaatc ctctctggca 60
ttggctttgg cccgacgttt aggtgctccc attctcagtg cagactctcg gcaagtctat 120
cgggggtttg atattggtac tgcgaaacct tctgtaacgg atcaagaaga tgttcctcac 180
tatctcattg atatatgtga ccctactgag actttgacgg ttgccgacta ccaagagcag 240
gctcaggcgc tcattgctca gtttcatact gaggggcaaa cgccgattct cgtggggggg 300
actggactgt atatccgggc gattgtggag gggctgaaga ttccgcgggt accgccgcaa 360
ctggagttgc gatcgcaact ccaatcccaa ggccaggtcc agatttacca atggctccag 420
caagtcgatc caccagcagc ccaaaaaatt catgcccatg accaagtccg taccctaaga 480
gcattagaag tcttttacac cacgggcatc cccctatcag cacagcaagg caagaatccc 540
ccgtcctatc ccatcttgca aattggctta gatattagtg atctggacca gcataccgac 600
atcattcagc agcgcacggc tgccatggtg gagcagggtt ggttgactga agtccaaaaa 660
ctcatcgacc actatggggt cgaactcccc ctcttagcaa ccctgggcta tcaagaaatg 720
aaggcctatc tgcatcagca aatcacccta gaagaagcaa ccgcccaaac tattcttcat 780
actcgtcaat tcgccaagcg ccaacgcacc tggtttagag cgaattctgg cattcactgg 840
tttgatgcca cgaattctga cttactttct ctagtttgga aggatattca aggtcaacct 900
tggctgtaa 909
<210>146
<211>302
<212>PRT
<213>Acaryochloris marina
<400>146
Met Thr Pro Gly Leu Ile Val Ile Cys Gly Pro Thr Ala Thr Gly Lys
1 5 10 15
Ser Ser Leu Ala Leu Ala Leu Ala Arg Arg Leu Gly Ala Pro Ile Leu
20 25 30
Ser Ala Asp Ser Arg Gln Val Tyr Arg Gly Phe Asp Ile Gly Thr Ala
35 40 45
Lys Pro Ser Val Thr Asp Gln Glu Asp Val Pro His Tyr Leu Ile Asp
50 55 60
Ile Cys Asp Pro Thr Glu Thr Leu Thr Val Ala Asp Tyr Gln Glu Gln
65 70 75 80
Ala Gln Ala Leu Ile Ala Gln Phe His Thr Glu Gly Gln Thr Pro Ile
85 90 95
Leu Val Gly Gly Thr Gly Leu Tyr Ile Arg Ala Ile Val Glu Gly Leu
100 105 110
Lys Ile Pro Arg Val Pro Pro Gln Leu Glu Leu Arg Ser Gln Leu Gln
115 120 125
Ser Gln Gly Gln Val Gln Ile Tyr Gln Trp Leu Gln Gln Val Asp Pro
130 135 140
Pro Ala Ala Gln Lys Ile His Ala His Asp Gln Val Arg Thr Leu Arg
145 150 155 160
Ala Leu Glu Val Phe Tyr Thr Thr Gly Ile Pro Leu Ser Ala Gln Gln
165 170 175
Gly Lys Asn Pro Pro Ser Tyr Pro Ile Leu Gln Ile Gly Leu Asp Ile
180 185 190
Ser Asp Leu Asp Gln His Thr Asp Ile Ile Gln Gln Arg Thr Ala Ala
195 200 205
Met Val Glu Gln Gly Trp Leu Thr Glu Val Gln Lys Leu Ile Asp His
210 215 220
Tyr Gly Val Glu Leu Pro Leu Leu Ala Thr Leu Gly Tyr Gln Glu Met
225 230 235 240
Lys Ala Tyr Leu His Gln Gln Ile Thr Leu Glu Glu Ala Thr Ala Gln
245 250 255
Thr Ile Leu His Thr Arg Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe
260 265 270
Arg Ala Asn Ser Gly Ile His Trp Phe Asp Ala Thr Asn Ser Asp Leu
275 280 285
Leu Ser Leu Val Trp Lys Asp Ile Gln Gly Gln Pro Trp Leu
290 295 300
<210>147
<211>885
<212>DNA
<213>多变鱼腥藻(Anabaena variabilis)
<400>147
atgactaaat taatcgtaat ttgtggagct acggcgacgg gtaaatctgg tttggctttg 60
aacttagcca tgcggttggg ttctgtgatt ttgagtgctg attctcgtca agtgtatcgt 120
gaatttgata ttggtacagc gaagccaacg ctggcagaac aaaaagccgt tcctcattat 180
ctaatagata tctgcactcc cagagaaacg atgacagttg cagattatca agaacaggca 240
caagcactaa ttaattctct gccagtttcg ccgttattgt tagtgggagg tactggttta 300
tatatacgtt ctattgtgca ggggatgaag atccccaggg ttgcgccgaa ttatgaattg 360
cgatcgcaac tcgaatcttt aggtcaaact acactctacg gcatattaca acaagttgat 420
cccgttgcgg ctcaaaagat tcatccccat gatcctgtac ggactttacg ggcagtagaa 480
gtattttacg ttactggcat tcccatatcg gcacagcaag gagaaaatcc cccagattac 540
cctattttac aaattggatt agattgtgaa atggaaagat tgagtgagcg cattcacaaa 600
cgcactgaac aaatgatagc agatggctta gttggagaag ttgaatatct gtgccaaaaa 660
tatggtgctg atttaccctt gttaaatact ttaggatatc aagaaattaa gcaatattta 720
gctggagaaa tttccttaga agcagccaaa gaattaatcg tactgcatac aagacaattt 780
gccaaacgcc aacgcacctg gtttagagcc tatccccaaa ttgagtggtt taatgcagat 840
gatgctgatt tattagatat tgtttggcag cgcatacaac agtag 885
<210>148
<211>294
<212>PRT
<213>多变鱼腥藻
<400>148
Met Thr Lys Leu Ile Val Ile Cys Gly Ala Thr Ala Thr Gly Lys Ser
1 5 10 15
Gly Leu Ala Leu Asn Leu Ala Met Arg Leu Gly Ser Val Ile Leu Ser
20 25 30
Ala Asp Ser Arg Gln Val Tyr Arg Glu Phe Asp Ile Gly Thr Ala Lys
35 40 45
Pro Thr Leu Ala Glu Gln Lys Ala Val Pro His Tyr Leu Ile Asp Ile
50 55 60
Cys Thr Pro Arg Glu Thr Met Thr Val Ala Asp Tyr Gln Glu Gln Ala
65 70 75 80
Gln Ala Leu Ile Asn Ser Leu Pro Val Ser Pro Leu Leu Leu Val Gly
85 90 95
Gly Thr Gly Leu Tyr Ile Arg Ser Ile Val Gln Gly Met Lys Ile Pro
100 105 110
Arg Val Ala Pro Asn Tyr Glu Leu Arg Ser Gln Leu Glu Ser Leu Gly
115 120 125
Gln Thr Thr Leu Tyr Gly Ile Leu Gln Gln Val Asp Pro Val Ala Ala
130 135 140
Gln Lys Ile His Pro His Asp Pro Val Arg Thr Leu Arg Ala Val Glu
145 150 155 160
Val Phe Tyr Val Thr Gly Ile Pro Ile Ser Ala Gln Gln Gly Glu Asn
165 170 175
Pro Pro Asp Tyr Pro Ile Leu Gln Ile Gly Leu Asp Cys Glu Met Glu
180 185 190
Arg Leu Ser Glu Arg Ile His Lys Arg Thr Glu Gln Met Ile Ala Asp
195 200 205
Gly Leu Val Gly Glu Val Glu Tyr Leu Cys Gln Lys Tyr Gly Ala Asp
210 215 220
Leu Pro Leu Leu Asn Thr Leu Gly Tyr Gln Glu Ile Lys Gln Tyr Leu
225 230 235 240
Ala Gly Glu Ile Ser Leu Glu Ala Ala Lys Glu Leu Ile Val Leu His
245 250 255
Thr Arg Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe Arg Ala Tyr Pro
260 265 270
Gln Ile Glu Trp Phe Asn Ala Asp Asp Ala Asp Leu Leu Asp Ile Val
275 280 285
Trp Gln Arg Ile Gln Gln
290
<210>149
<211>909
<212>DNA
<213>无类囊体蓝藻(Gloeobacter violaceus)
<400>149
gtgaaaaaaa tcgtcgtcgt ctgcggcccc accgccgccg gcaaaagcca actgggaatg 60
catctggccc agcacctggg ggtaccggtg ctgagcgccg attcccggca ggtctaccgc 120
gagttcgaca tcggcactgc taaaccgacc cgtgaagaac aacgccgcgt cgagcactgc 180
ctcatcgatg tcgcctggcc cacggaacat ttcaacgtgg cgcgctaccg ggaactggcc 240
gatgcggaga ttgaccggct gacccggcaa ggcaaacccg ccctactggt gggaggcagt 300
ggtttgtacc tgcgcgcggt atcgggcgga ctcgaaccgc ccgccgtccc ccccgacccg 360
gctttgcgcg cccgcctggc ggttgagcgg ttggacgcac tttaccagcg acttcaacag 420
ctcgatcccg aatccgcggg gcgcatccac cccaacgacc aggtgcgcat cgagcgcgcc 480
ctcgaagtgt gcctgacaac cggccaaccg ctgagtgccc agcgccgcct gcgggcgcga 540
gatttttcgc tcctcgccct gggggtgggc agcggccggg aagcgctggt gcgccgcatc 600
gagcagcgca cccaccggat gatcgaagcg ggttggctgg aggaagtcga gtatctgcgc 660
gccaaatacg gccccgattt gccgcttctg agcaccctcg gttacgccga gttgggggcg 720
tacctggagg agcgatggga tctggcggag gcgctccagc agatcgtcgt gcatacccgc 780
cagttcgcca agcgccagat gacctggttt cgcgccgagc ccgatgttca ttggctcgat 840
gaatcggcag gacggcagac acttgaacaa cagagtatcg aacaggttga gcgctttctt 900
gccggttga 909
<210>150
<211>302
<212>PRT
<213>无类囊体蓝藻
<400>150
Met Lys Lys Ile Val Val Val Cys Gly Pro Thr Ala Ala Gly Lys Ser
1 5 10 15
Gln Leu Gly Met His Leu Ala Gln His Leu Gly Val Pro Val Leu Ser
20 25 30
Ala Asp Ser Arg Gln Val Tyr Arg Glu Phe Asp Ile Gly Thr Ala Lys
35 40 45
Pro Thr Arg Glu Glu Gln Arg Arg Val Glu His Cys Leu Ile Asp Val
50 55 60
Ala Trp Pro Thr Glu His Phe Asn Val Ala Arg Tyr Arg Glu Leu Ala
65 70 75 80
Asp Ala Glu Ile Asp Arg Leu Thr Arg Gln Gly Lys Pro Ala Leu Leu
85 90 95
Val Gly Gly Ser Gly Leu Tyr Leu Arg Ala Val Ser Gly Gly Leu Glu
100 105 110
Pro Pro Ala Val Pro Pro Asp Pro Ala Leu Arg Ala Arg Leu Ala Val
115 120 125
Glu Arg Leu Asp Ala Leu Tyr Gln Arg Leu Gln Gln Leu Asp Pro Glu
130 135 140
Ser Ala Gly Arg Ile His Pro Asn Asp Gln Val Arg Ile Glu Arg Ala
145 150 155 160
Leu Glu Val Cys Leu Thr Thr Gly Gln Pro Leu Ser Ala Gln Arg Arg
165 170 175
Leu Arg Ala Arg Asp Phe Ser Leu Leu Ala Leu Gly Val Gly Ser Gly
180 185 190
Arg Glu Ala Leu Val Arg Arg Ile Glu Gln Arg Thr His Arg Met Ile
195 200 205
Glu Ala Gly Trp Leu Glu Glu Val Glu Tyr Leu Arg Ala Lys Tyr Gly
210 215 220
Pro Asp Leu Pro Leu Leu Ser Thr Leu Gly Tyr Ala Glu Leu Gly Ala
225 230 235 240
Tyr Leu Glu Glu Arg Trp Asp Leu Ala Glu Ala Leu Gln Gln Ile Val
245 250 255
Val His Thr Arg Gln Phe Ala Lys Arg Gln Met Thr Trp Phe Arg Ala
260 265 270
Glu Pro Asp Val His Trp Leu Asp Glu Ser Ala Gly Arg Gln Thr Leu
275 280 285
Glu Gln Gln Ser Ile Glu Gln Val Glu Arg Phe Leu Ala Gly
290 295 300
<210>151
<211>936
<212>DNA
<213>铜锈微囊藻(Microcystis aeruginosa)
<400>151
atgtcaacta ttctgattgt tatttgtggg gcaactgcca caggaaaatc cagtttagcc 60
ctacaattgg ccaaaaagtt taattcagta attctcagtg ccgattcccg acaaatctat 120
cgagaattta atattggtac tgctaaacct agcttagaag aatgtcagcg gattccccat 180
tatctaattg atatttgtga tcctcaagat aattttaccc tagcacagta ccaagaacag 240
gctgagaatt taatcagcaa tctccactat tctcctctgt ttttggtcgg tggtacggga 300
ctttatatca agtccattgt caaaggttta aaaataccga gggtttctce tcaagcggat 360
ttaagacggc aattacaagc tttaggacaa tcctatcttt atcaaattct gatgcaggta 420
gatgaagagg ccgctaaaaa aattcatccc catgatcagg tcaggacttt acgcgcttta 480
gaggtttttt atgtgacagg aaaacctatc tctagtcagc agggagaaaa tccccccact 540
tatccgattt tacaaattgg tttagactgt tccccagaaa gtttagacaa gcggatcact 600
gttcgtactc atcaaatgat cgccaaggga ttagtagcag aggtgcagaa tttgggggat 660
aaatacggtt gggatttgcc acttttgcag actttgggtt atgcagagat taagcaatat 720
ctcctcggag aagtttcttt agaacaggcg atcgatctaa ttatcctcca cacccgtcaa 780
tttgccaaac gtcagcgcac ttggtttcgt gccgatgctg atattgtctg gtttcccgtc 840
gataaagaaa atttattgga attggtggaa cgaaaaatta ttttgtttct tgaggggttg 900
tcaaggcccg atataaaaac acatcatcct gattaa 936
<210>152
<211>311
<212>PRT
<213>铜锈微囊藻
<400>152
Met Ser Thr Ile Leu Ile Val Ile Cys Gly Ala Thr Ala Thr Gly Lys
1 5 10 15
Ser Ser Leu Ala Leu Gln Leu Ala Lys Lys Phe Asn Ser Val Ile Leu
20 25 30
Ser Ala Asp Ser Arg Gln Ile Tyr Arg Glu Phe Asn Ile Gly Thr Ala
35 40 45
Lys Pro Ser Leu Glu Glu Cys Gln Arg Ile Pro His Tyr Leu Ile Asp
50 55 60
Ile Cys Asp Pro Gln Asp Asn Phe Thr Leu Ala Gln Tyr Gln Glu Gln
65 70 75 80
Ala Glu Asn Leu Ile Ser Asn Leu His Tyr Ser Pro Leu Phe Leu Val
85 90 95
Gly Gly Thr Gly Leu Tyr Ile Lys Ser Ile Val Lys Gly Leu Lys Ile
100 105 110
Pro Arg Val Ser Pro Gln Ala Asp Leu Arg Arg Gln Leu Gln Ala Leu
115 120 125
Gly Gln Ser Tyr Leu Tyr Gln Ile Leu Met Gln Val Asp Glu Glu Ala
130 135 140
Ala Lys Lys Ile His Pro His Asp Gln Val Arg Thr Leu Arg Ala Leu
145 150 155 160
Glu Val Phe Tyr Val Thr Gly Lys Pro Ile Ser Ser Gln Gln Gly Glu
165 170 175
Asn Pro Pro Thr Tyr Pro Ile Leu Gln Ile Gly Leu Asp Cys Ser Pro
180 185 190
Glu Ser Leu Asp Lys Arg Ile Thr Val Arg Thr His Gln Met Ile Ala
195 200 205
Lys Gly Leu Val Ala Glu Val Gln Asn Leu Gly Asp Lys Tyr Gly Trp
210 215 220
Asp Leu Pro Leu Leu Gln Thr Leu Gly Tyr Ala Glu Ile Lys Gln Tyr
225 230 235 240
Leu Leu Gly Glu Val Ser Leu Glu Gln Ala Ile Asp Leu Ile Ile Leu
245 250 255
His Thr Arg Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe Arg Ala Asp
260 265 270
Ala Asp Ile Val Trp Phe Pro Val Asp Lys Glu Asn Leu Leu Glu Leu
275 280 285
Val Glu Arg Lys Ile Ile Leu Phe Leu Glu Gly Leu Ser Arg Pro Asp
290 295 300
Ile Lys Thr His His Pro Asp
305 310
<210>153
<211>888
<212>DNA
<213>念球藻属物种(Nostoc sp.)
<400>153
atgactaaat taatcgtaat ttgtggtgct acggcgacag gtaaatctgg tttggctttg 60
aacttagcta tgcggctggg ttctgtgatt ctgagtgctg attctcgtca agtgtatcgt 120
gaatttgata ttggtacagc gaaaccaacg ctagcagaac aaagagccgt ccctcattat 180
ttaatagata tctgcgctcc tagagagacg atgacagttg cagattatca agaacaggca 240
caagcattga ttaattcttt gccagtctcg ccgctattgt tagtgggagg cacaggttta 300
tatatacgtt ctattgtgca agggatgaag atccccaggg ttgcgccgaa ttatgaattg 360
cgatcgcaac tcgcctctct aggtcaaact acactctacg gcatattaca acaagttgat 420
cccattgctg cccaaaaaat tcatcccaat gaccctgtgc gaactttacg cgcagtagaa 480
gttttttaca ttactggcat tcccatatcg gcacagcaag gagagaatcc gccagattac 540
ccaattttac agattgggtt agattgtgaa atggaaagac tgactgagcg gattcacaaa 600
cgcactgagc aaatgataac agatggctta gttggagaag ttgaatatct ttgccaaaaa 660
tatggtgctg agttaccctt gttaaatact ttaggttatc aagaaataaa gcaatattta 720
actggggaaa tttccttaga agcagccaaa gaattaactg ttttgcatac acgacaattt 780
gccaaacgcc aacgcacctg gtttagagca tctccccaaa ttgagtggtt taatgcagat 840
catcctgatt tattagatat tgtttgccag cacatacaac aaccgtag 888
<210>154
<211>295
<212>PRT
<213>念球藻属物种
<400>154
Met Thr Lys Leu Ile Val Ile Cys Gly Ala Thr Ala Thr Gly Lys Ser
1 5 10 15
Gly Leu Ala Leu Asn Leu Ala Met Arg Leu Gly Ser Val Ile Leu Ser
20 25 30
Ala Asp Ser Arg Gln Val Tyr Arg Glu Phe Asp Ile Gly Thr Ala Lys
35 40 45
Pro Thr Leu Ala Glu Gln Arg Ala Val Pro His Tyr Leu Ile Asp Ile
50 55 60
Cys Ala Pro Arg Glu Thr Met Thr Val Ala Asp Tyr Gln Glu Gln Ala
65 70 75 80
Gln Ala Leu Ile Asn Ser Leu Pro Val Ser Pro Leu Leu Leu Val Gly
85 90 95
Gly Thr Gly Leu Tyr Ile Arg Ser Ile Val Gln Gly Met Lys Ile Pro
100 105 110
Arg Val Ala Pro Asn Tyr Glu Leu Arg Ser Gln Leu Ala Ser Leu Gly
115 120 125
Gln Thr Thr Leu Tyr Gly Ile Leu Gln Gln Val Asp Pro Ile Ala Ala
130 135 140
Gln Lys Ile His Pro Asn Asp Pro ValArg Thr Leu Arg Ala Val Glu
145 150 155 160
Val Phe Tyr Ile Thr Gly Ile Pro Ile Ser Ala Gln Gln Gly Glu Asn
165 170 175
Pro Pro Asp Tyr Pro Ile Leu Gln Ile Gly Leu Asp Cys Glu Met Glu
180 185 190
Arg Leu Thr Glu Arg Ile His Lys Arg Thr Glu Gln Met Ile Thr Asp
195 200 205
Gly Leu Val Gly Glu Val Glu Tyr Leu Cys Gln Lys Tyr Gly Ala Glu
210 215 220
Leu Pro Leu Leu Asn Thr Leu Gly Tyr Gln Glu Ile Lys Gln Tyr Leu
225 230 235 240
Thr Gly Glu Ile Ser Leu Glu Ala Ala Lys Glu Leu Thr Val Leu His
245 250 255
Thr Arg Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe Arg Ala Ser Pro
260 265 270
Gln Ile Glu Trp Phe Asn Ala Asp His Pro Asp Leu Leu Asp Ile Val
275 280 285
Cys Gln His Ile Gln Gln Pro
290 295
<210>155
<211>900
<212>DNA
<213>海洋原绿球藻(Prochlorococcus marinus)
<400>155
atgcaaaacc caaaaccatt ggtaatagtt ttgcttggac caacagcaag cgggaaaact 60
gatctagcta tacaaatagc caaaaagata aaagtaagta ttcacaatat tgattcacgt 120
caattatata aaggaatgaa tattggtact gctaaaccaa cgatagagca gcaagaagaa 180
ataaagcatt atctactaga tcttaaagat cctaataacc ccatcacatt gcatgaattc 240
aaaaaagaag cagagttgag cctaaagaat atttttagca aagaaaagtg tggctttttg 300
gtcggcggaa gtggcctcta tttaaaatct ctaacaagtg gtttatgccc accatctgtt 360
ccagcacaag aaaaactccg gaaagagttt agaagacttg gacaaaaaga gtgtcatcaa 420
atactgaaaa aatgtgatcc cattgcttgg gaaaaaattt cccctagaga ttctattaga 480
acaataagag cattagaagt tttctattcc actggacaga caataagctc attaaaaact 540
ttaaagcctc ctgactggaa tttattagag ctagggttag atccaagaaa tcttcaacaa 600
agaattgcta aaagaacaaa aatattgttc caaaaaggtc ttattgatga aactaaagct 660
ttaattcatc aatatggaga agatcttcct ttgctacaga caattggata caaagaagct 720
tgcacagtaa taaaaggcga atattcaatt acagaagcaa tcgaaatcac aacccaacga 780
acgaatcaat ttgctaagaa gcaaaggact tggtttagaa gacaacataa tccaaaatgg 840
ctaaatgaaa aaaactctct tgaagaagct ctctctttga tccaaaacgt tatagggtga 900
<210>156
<211>299
<212>PRT
<213>海洋原绿球藻
<400>156
Met Gln Asn Pro Lys Pro Leu Val Ile Val Leu Leu Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Asp Leu Ala Ile Gln Ile Ala Lys Lys Ile Lys Val
20 25 30
Ser Ile His Asn Ile Asp Ser Arg Gln Leu Tyr Lys Gly Met Asn Ile
35 40 45
Gly Thr Ala Lys Pro Thr Ile Glu Gln Gln Glu Glu Ile Lys His Tyr
50 55 60
Leu Leu Asp Leu Lys Asp Pro Asn Asn Pro Ile Thr Leu His Glu Phe
65 70 75 80
Lys Lys Glu Ala Glu Leu Ser Leu Lys Asn Ile Phe Ser Lys Glu Lys
85 90 95
Cys Gly Phe Leu Val Gly Gly Ser Gly Leu Tyr Leu Lys Ser Leu Thr
100 105 110
Ser Gly Leu Cys Pro Pro Ser Val Pro Ala Gln Glu Lys Leu Arg Lys
115 120 125
Glu Phe Arg Arg Leu Gly Gln Lys Glu Cys His Gln Ile Leu Lys Lys
130 135 140
Cys Asp Pro Ile Ala Trp Glu Lys Ile Ser Pro Arg Asp Ser Ile Arg
145 150 155 160
Thr Ile Arg Ala Leu Glu Val Phe Tyr Ser Thr Gly Gln Thr Ile Ser
165 170 175
Ser Leu Lys Thr Leu Lys Pro Pro Asp Trp Asn Leu Leu Glu Leu Gly
180 185 190
Leu Asp Pro Arg Asn Leu Gln Gln Arg Ile Ala Lys Arg Thr Lys Ile
195 200 205
Leu Phe Gln Lys Gly Leu Ile Asp Glu Thr Lys Ala Leu Ile His Gln
210 215 220
Tyr Gly Glu Asp Leu Pro Leu Leu Gln Thr Ile Gly Tyr Lys Glu Ala
225 230 235 240
Cys Thr Val Ile Lys Gly Glu Tyr Ser Ile Thr Glu Ala Ile Glu Ile
245 250 255
Thr Thr Gln Arg Thr Asn Gln Phe Ala Lys Lys Gln Arg Thr Trp Phe
260 265 270
Arg Arg Gln His Asn Pro Lys Trp Leu Asn Glu Lys Asn Ser Leu Glu
275 280 285
Glu Ala Leu Ser Leu Ile Gln Asn Val Ile Gly
290 295
<210>157
<211>900
<212>DNA
<213>海洋原绿球藻
<400>157
atgtcgtcat caaaaccact cataattgtt ctccttggac ctactgctag cgggaaaact 60
gcactaggga tagaaatagc tgagcatctg ggactcgaga tacataatgt tgattctcgt 120
caggtttata tggatatgga catcgggacc gcaaaaccat cccaagaaca gcaaaaacgt 180
ataagacatt tcttgattga tttaaagccg cctaatgaaa aaatgacaat gcatgatttc 240
cataaaacag caagagtcag cttggataat gcattaaata aaacaaacgt tggattactt 300
gttggtggaa gtggtctgta tctcaaagcc ttaactagcg gtctatgccc tccatccatt 360
cctcccgaat caagttttag gaaacaatta catgacattg gtcaagagca gtgttatcaa 420
cttctccaaa gctgcgaccc actgagcgcg aaaaccattg ctccatcaga ctcagtcaga 480
acaacaagag cactagaagt tttttatgca actggtcaat ctaaaacttc ccttcaatct 540
tccaaaccac ctccctggag gttattagag ctaggactta atccaagtaa tctcaatgat 600
cgcattgcac aaagaacaga aaacattttt caaaacggtc ttattgaaga aactgaacac 660
ctcattggaa aatttggaaa ggaattgccc ttgctaaaca ctattggata tgcagaggca 720
tctcaaatga ttgatggcaa gcttccctta aatgacgcca tatttcaaac aaataaaaga 780
actaagcaat ttgcaaaaag acaaaaaact tggtttaggg gtcaacacaa tccaaaatgg 840
ttaaatgaga aaaacccact tagtgaagct ctctctttga tccataacgt tataaggtga 900
<210>158
<211>299
<212>PRT
<213>海洋原绿球藻
<400>158
Met Ser Ser Ser Lys Pro Leu Ile Ile Val Leu Leu Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Ala Leu Gly Ile Glu Ile Ala Glu His Leu Gly Leu
20 25 30
Glu Ile His Asn Val Asp Ser Arg Gln Val Tyr Met Asp Met Asp Ile
35 40 45
Gly Thr Ala Lys Pro Ser Gln Glu Gln Gln Lys Arg IIe Arg His Phe
50 55 60
Leu Ile Asp Leu Lys Pro Pro Asn Glu Lys Met Thr Met His Asp Phe
65 70 75 80
His Lys Thr Ala Arg Val Ser Leu Asp Asn Ala Leu Asn Lys Thr Asn
85 90 95
Val Gly Leu Leu Val Gly Gly Ser Gly Leu Tyr Leu Lys Ala Leu Thr
100 105 110
Ser Gly Leu Cys Pro Pro Set Ile Pro Pro Glu Ser Ser Phe Arg Lys
115 120 125
Gln Leu His Asp Ile Gly Gln Glu Gln Cys Tyr Gln Leu Leu Gln Ser
130 135 140
Cys Asp Pro Leu Ser Ala Lys Thr Ile Ala Pro Ser Asp Ser Val Arg
145 150 155 160
Thr Thr Arg Ala Leu Glu Val Phe Tyr Ala Thr Gly Gln Ser Lys Thr
165 170 175
Ser Leu Gln Ser Ser Lys Pro Pro Pro Trp Arg Leu Leu Glu Leu Gly
180 185 190
Leu Asn Pro Ser Asn Leu Asn Asp Arg Ile Ala Gln Arg Thr Glu Asn
195 200 205
Ile Phe Gln Asn Gly Leu Ile Glu Glu Thr Glu His Leu Ile Gly Lys
210 215 220
Phe Gly Lys Glu Leu Pro Leu Leu Asn Thr Ile Gly Tyr Ala Gtu Ala
225 230 235 240
Ser Gln Met Ile Asp Gly Lys Leu Pro Leu Asn Asp Ala Ile Phe Gln
245 250 255
Thr Asn Lys Arg Thr Lys Gln Phe Ala Lys Arg Gln Lys Thr Trp Phe
260 265 270
Arg Gly Gln His Asn Pro Lys Trp Leu Asn Glu Lys Asn Pro Leu Ser
275 280 285
Glu Ala Leu Ser Leu Ile His Asn Val Ile Arg
290 295
<210>159
<211>900
<212>DNA
<213>海洋原绿球藻
<400>159
atgtcttcat atccaactcc tgtaataatt ttaattgggc caactgcaag tggcaaaaca 60
gaattagcta ttaaaattgc agaatatttt aaaactagta ttcacaatat cgattcgaga 120
caaatatata aatttatgga tattggaacc gccaagccat ctaaagacca acaaaaaaaa 180
ataaagcatt ttttaataga tatagaggag ccaatcaatc aaattaatgt gcaacaattt 240
caaaaaattg ctcaaaaatc aataaaaaga gaaataaatc aagacaatct accttttctt 300
gttgggggga gtggtttgta tatgaactca ataacaaaag gtttttttat accagatatt 360
cctcctcaaa ataatttgag aatacaatta gaagaacttg gccaaaaaaa atgttgggaa 420
ctcttaaata attgtgatcc attatcaaca aaaaacatca gttttgctga tcgcattaga 480
acaataagag ctttagaagt tttctatgta acgggtaaac ctttgtcaac tctaaaagtt 540
caaaatccgc ctaattggaa agtactagag cttggattag atagagataa cttaaaagaa 600
agaattttac aaagaacaaa aaatattttt ctttcgggaa ttattgaaga aacaaaccac 660
cttatctcta aatacggatt tgatttgcca atattagaaa ccattggtta tcgagaagct 720
aaggatgttt taaagaacca ttcaacaatc gacaaggcga ttgaattaac tacgacaaaa 780
acgatccaat ttgccaaaag acaaaaaact tggtttcgca ataaaaataa tcctatttgg 840
ctgaataaca aaaacctact aaaagatgca ataattaaga tagagtcttt tttaagctaa 900
<210>160
<211>299
<212>PRT
<213>海洋原绿球藻
<400>160
Met Ser Ser Tyr Pro Thr Pro Val Ile Ile Leu Ile Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Glu Leu Ala Ile Lys Ile Ala Glu Tyr Phe Lys Thr
20 25 30
Ser Ile His Asn Ile Asp Ser Arg Gln Ile Tyr Lys Phe Met Asp Ile
35 40 45
Gly Thr Ala Lys Pro Ser Lys Asp Gln Gln Lys Lys Ile Lys His Phe
50 55 60
Leu Ile Asp Ile Glu Glu Pro Ile Asn Gln Ile Asn Val Gln Gln Phe
65 70 75 80
Gln Lys Ile Ala Gln Lys Ser Ile Lys Arg Glu Ile Asn Gln Asp Asn
85 90 95
Leu Pro Phe Leu Val Gly Gly Ser Gly Leu Tyr Met Asn Ser Ile Thr
100 105 110
Lys Gly Phe Phe Ile Pro Asp Ile Pro Pro Gln Asn Asn Leu Arg Ile
115 120 125
Gln Leu Glu Glu Leu Gly Gln Lys Lys Cys Trp Glu Leu Leu Asn Asn
130 135 140
Cys Asp Pro Leu Ser Thr Lys Asn Ile Ser Phe Ala Asp Arg Ile Arg
145 150 155 160
Thr Ile Arg Ala Leu Glu Val Phe Tyr Val Thr Gly Lys Pro Leu Ser
165 170 175
Thr Leu Lys Val Gln Asn Pro Pro Asn Trp Lys Val Leu Glu Leu Gly
180 185 190
Leu Asp Arg Asp Asn Leu Lys Glu Arg Ile Leu Gln Arg Thr Lys Asn
195 200 205
Ile Phe Leu Ser Gly Ile Ile Glu G1u Thr Asn His Leu Ile Ser Lys
210 215 220
Tyr Gly Phe Asp Leu Pro Ile Leu Glu Thr Ile Gly Tyr Arg Glu Ala
225 230 235 240
Lys Asp Val Leu Lys Asn His Ser Thr Ile Asp Lys Ala Ile Glu Leu
245 250 255
Thr Thr Thr Lys Thr Ile Gln Phe Ala Lys Arg Gln Lys Thr Trp Phe
260 265 270
Arg Asn Lys Asn Asn Pro Ile Trp Leu Asn Asn Lys Asn Leu Leu Lys
275 280 285
Asp Ala Ile Ile Lys Ile Glu Ser Phe Leu Ser
290 295
<210>161
<211>900
<212>DNA
<213>海洋原绿球藻
<400>161
atgtcttcac atccacctca tgtaatagtt ttaattgggc ctactgcaag tggcaaaaca 60
gaattagcta ttgaaattgc agaatatttt aaaactaata tacacaatat cgattcaagg 120
caaatatata agtccatgga tattggaaca gccaagccat ctaaaatcca acaaaaaaaa 180
atacggcatt ttttaataga tattgaagaa cctatcaatc caattaatgt aaaacaattt 240
caagaaattg ctcagaaatc gatcaaagaa gaaattaaaa aagataaatt accttttctt 300
gttggaggaa gtggtttgta tatgaactca ataacaaaag gttttttcgt accagatgtc 360
cctcctcaaa ataatttaag aaaacaatta gaagaacttg gtcaggaaaa atgttgggac 420
ctgttagaaa attgtgatcc attatcaaca aaaaaaatca attttgctga ccacgttcga 480
acaataagag ctttagaagt cttctacgta acaggtaagc ctttatcaac tctgaaagtt 540
cagaaaccac ctaactggaa aatcttagag cttggattag acagaggtaa tttaaaagaa 600
agaatttttc aaagaacaaa aaatatgttt ttatctggaa taattgatga gacgaatcac 660
cttatctcta aatacggatt tgatttgcca atattagaaa caattggcta tcgagaagct 720
agaaatgttt taaataacca ttcaacaatt gacaaagcga ttgagttaac tgcaacaaaa 780
acaatccaat ttgccaaaag acaaaaaact tggtttcgta ataaaaataa tcctctttgg 840
cttaataaca aaaacccgct aaaagatgca ataattaaga tagagtcttt tttaagctaa 900
<210>162
<211>299
<212>PRT
<213>海洋原绿球藻
<400>162
Met Ser Ser His Pro Pro His Val Ile Val Leu Ile Gly Pro ThrAla
1 5 10 15
Ser Gly Lys Thr Glu Leu Ala Ile Glu Ile Ala Glu Tyr Phe Lys Thr
20 25 30
Asn Ile His Asn Ile Asp Ser Arg Gln Ile Tyr Lys Ser Met Asp Ile
35 40 45
Gly Thr Ala Lys Pro Ser Lys Ile Gln Gln Lys Lys Ile Arg His Phe
50 55 60
Leu Ile Asp Ile Glu Glu Pro Ile Asn Pro Ile Asn Val Lys Gln Phe
65 70 75 80
Gln Glu Ile Ala Gln Lys Ser Ile Lys Glu Glu Ile Lys Lys Asp Lys
85 90 95
Leu Pro Phe Leu Val Gly Gly Ser Gly Leu Tyr Met Asn Ser Ile Thr
100 105 110
Lys Gly Phe Phe Val Pro Asp Val Pro Pro Gln Asn Asn Leu Arg Lys
115 120 125
Gln Leu Glu Glu Leu Gly Gln Glu Lys Cys Trp Asp Leu Leu Glu Asn
130 135 140
Cys Asp Pro Leu Ser Thr Lys Lys Ile Asn Phe Ala Asp His Val Arg
145 150 155 160
Thr Ile Arg Ala Leu Glu Val Phe Tyr Val Thr Gly Lys Pro Leu Ser
165 170 175
Thr Leu Lys Val Gln Lys Pro Pro Asn Trp Lys Ile Leu Glu Leu Gly
180 185 190
Leu Asp Arg Gly Asn Leu Lys Glu Arg Ile Phe Gln Arg Thr Lys Asn
195 200 205
Met Phe Leu Ser Gly Ile Ile Asp Glu Thr Asn His Leu Ile Ser Lys
210 215 220
Tyr Gly Phe Asp Leu Pro Ile Leu Glu Thr Ile Gly Tyr Arg Glu Ala
225 230 235 240
Arg Asn Val Leu Asn Asn His Ser Thr Ile Asp Lys Ala Ile Glu Leu
245 250 255
Thr Ala Thr Lys Thr Ile Gln Phe Ala Lys Arg Gln Lys Thr Trp Phe
260 265 270
Arg Asn Lys Asn Asn Pro Leu Trp Leu Asn Asn Lys Asn Pro Leu Lys
275 280 285
Asp Ala Ile Ile Lys Ile Glu Ser Phe Leu Ser
290 295
<210>163
<211>900
<212>DNA
<213>海洋原绿球藻
<400>163
atgcccgcct ctaagccgct tctgattgcg ctgctagggc cgacagcaag cggcaaaaca 60
tgcctggcct tacagctggc agaacagctg aagctgagcg tgctcaatgt ggattcccgc 120
cagctctaca tcggcatgga cgttggcacc gccaagccaa ccaaagaaca acagcggcgc 180
gtgcaacatc acctgattga tcttcgtcgc ccagatcaac cgatcaccct gcaggaattc 240
caggcagccg cgcaactgat ccttgcgcaa aagctaaggg aacaaaacat gccatttcta 300
gttggcggca gtggtcttta tctcaaagct ctcacttgtg ggctgcgccc accagcagtg 360
ccaccccaac cagagctccg caagcagtta ggcgagctag gacaacgcac ctgccaccac 420
ctactccaag ccgccgatcc caccgctgcc agccgaattg cccctgctga tgccatgcgc 480
accaaacggg ctctggaagt ggtttatgcc actggcaaac cgatcaccac tcaacaaggc 540
tcaagcccac ccccttggcg cgttctggaa ctaggcctcg acccacacaa cctgcgcgaa 600
cgcattggcc aacgcacaac gcagctctat gccaatggtc tcatcgaaga aaccgaacac 660
ttgagtcagt gctacgacag cgatctgccc ttgctgcaaa caatcggcta tggggaagca 720
ctaaaggtga ttcaaggcct actcaaccgt gaacaggcca tcgccctgac cacacgtcgt 780
actcagcagt tcgccaagcg tcagcgcaca tggttccgca gacaacatca cccctattgg 840
ctcaaaggtg aggaaccgct gagcgaagcg ctatcactga ttcaagcggg tctaaggtga 900
<210>164
<211>299
<212>PRT
<213>海洋原绿球藻
<400>164
Met Pro Ala Ser Lys Pro Leu Leu Ile Ala Leu Leu Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Cys Leu Ala Leu Gln Leu Ala Glu Gln Leu Lys Leu
20 25 30
Ser Val Leu Asn Val Asp Ser Arg Gln Leu Tyr Ile Gly Met Asp Val
35 40 45
Gly Thr Ala Lys Pro Thr Lys Glu Gln Gln Arg Arg Val Gln His His
50 55 60
Leu Ile Asp Leu Arg Arg Pro Asp Gln Pro Ile Thr Leu Gln Glu Phe
65 70 75 80
Gln Ala Ala Ala Gln Leu Ile Leu Ala Gln Lys Leu Arg Glu Gln Asn
85 90 95
Met Pro Phe Leu Val Gly Gly Ser Gly Leu Tyr Leu Lys Ala Leu Thr
100 105 110
Cys Gly Leu Arg Pro Pro Ala Val Pro Pro Gln Pro Glu Leu Arg Lys
115 120 125
Gln Leu Gly Glu Leu Gly Gln Arg Thr Cys His His Leu Leu Gln Ala
130 135 140
Ala Asp Pro Thr Ala Ala Ser Arg Ile Ala Pro Ala Asp Ala Met Arg
145 150 155 160
Thr Lys Arg Ala Leu Glu Val Val Tyr Ala Thr Gly Lys Pro Ile Thr
165 170 175
Thr Gln Gln Gly Ser Ser Pro Pro Pro Trp Arg Val Leu Glu Leu Gly
180 185 190
Leu Asp Pro His Asn Leu Arg Glu Arg Ile Gly Gln Arg Thr Thr Gln
195 200 205
Leu Tyr Ala Asn Gly Leu Ile Glu Glu Thr Glu His Leu Ser Gln Cys
210 215 220
Tyr Asp Ser Asp Leu Pro Leu Leu Gln Thr Ile Gly Tyr Gly Glu Ala
225 230 235 240
Leu Lys Val Ile Gln Gly Leu Leu Asn Arg Glu Gln Ala Ile Ala Leu
245 250 255
Thr Thr Arg Arg Thr Gln Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe
260 265 270
Arg Arg Gln His His Pro Tyr Trp Leu Lys Gly Glu Glu Pro Leu Ser
275 280 285
Glu Ala Leu Ser Leu Ile Gln Ala Gly Leu Arg
290 295
<210>165
<211>900
<212>DNA
<213>海洋原绿球藻
<400>165
atgtcttctt atccccctca tgtaatagtt ctaattggag ccacagcaag tgggaaaaca 60
gaattagcta tcgaaattgc agaatatttt aaaactcgta tacataatat cgattcgaga 120
caaatttata aatctatgga tattggaaca gccaaaccat ctgagaatca acaaaaaaaa 180
ataaagcatt ttctaataga tatagaagaa cctattaatc caattaatgt aaaacaattt 240
caagaaattg ctcaaaaatc aatacaaaaa gaaattaagc aaaataattt acctctactt 300
gttggaggaa gtggtttgta tatgaacgca ataaccaaag gtttttttgt gccggatgtc 360
cctccgcaaa ataatttgag agagcaattg gaggaacttg gtcaaaaaga atgttgggag 420
cttttaaaaa attgtgatcc aatttcggca aaaaaaatca atcttgctga tcaaattaga 480
acaataagag ctttggaagt attttatgta accggtaaac ctttatcttc tcaaaaagtt 540
caaaaaccgc ctcaatggaa aatactagag cttggattaa atagagataa tttaaaagaa 600
agaatttcac gaagaacaaa aaatatgttt ttatctggaa ttattgaaga gacgaaaaat 660
cttatttctc gatatggatc tgatttacca atattagaaa ctattggtta ccgtgaagct 720
aaagatgttt taaataataa tttaccgatt gataaggcga ttgagttaac taatataaaa 780
acgaaccaat ttgccaaaag gcagaaaaca tggtttcgta ataaaaataa tcctatttgg 840
cttaataaca aaaacctgct aaaagatgca ataattaaga tagagtcttt tttagactaa 900
<210>166
<211>299
<212>PRT
<213>海洋原绿球藻
<400>166
Met Ser Ser Tyr Pro Pro His Val Ile Val Leu Ile Gly Ala Thr Ala
1 5 10 15
Ser Gly Lys Thr Glu Leu Ala Ile Glu Ile Ala Glu Tyr Phe Lys Thr
20 25 30
Arg Ile His Asn Ile Asp Ser Arg Gln Ile Tyr Lys Ser Met Asp Ile
35 40 45
Gly Thr Ala Lys Pro Ser Glu Asn Gln Gln Lys Lys Ile Lys His Phe
50 55 60
Leu Ile Asp Ile Glu Glu Pro Ile Asn Pro Ile Asn Val Lys Gln Phe
65 70 75 80
Gln Glu Ile Ala Gln Lys Ser Ile Gln Lys Glu Ile Lys Gln Asn Asn
85 90 95
Leu Pro Leu Leu Val Gly Gly Ser Gly Leu Tyr Met Asn Ala Ile Thr
100 105 110
Lys Gly Phe Phe Val Pro Asp Val Pro Pro Gln Asn Asn Leu Arg Glu
115 120 125
Gln Leu Glu Glu Leu Gly Gln Lys Glu Cys Trp Glu Leu Leu Lys Asn
130 135 140
Cys Asp Pro Ile Ser Ala Lys Lys Ile Asn Leu Ala Asp Gln Ile Arg
145 150 155 160
Thr Ile Arg Ala Leu Glu Val Phe Tyr Val Thr Gly Lys Pro Leu Ser
165 170 175
Ser Gln Lys Val Gln Lys Pro Pro Gln Trp Lys Ile Leu Glu Leu Gly
180 185 190
Leu Asn Arg Asp Asn Leu Lys Glu Arg Ile Ser Arg Arg Thr Lys Asn
195 200 205
Met Phe Leu Ser Gly Ile Ile Glu Glu Thr Lys Asn Leu Ile Ser Arg
210 215 220
Tyr Gly Ser Asp Leu Pro Ile Leu Glu Thr Ile Gly Tyr Arg Glu Ala
225 230 235 240
Lys Asp Val Leu Asn Asn Asn Leu Pro Ile Asp Lys Ala Ile Glu Leu
245 250 255
Thr Asn Ile Lys Thr Asn Gln Phe Ala Lys Arg Gln Lys Thr Trp Phe
260 265 270
Arg Asn Lys Asn Asn Pro Ile Trp Leu Asn Asn Lys Asn Leu Leu Lys
275 280 285
Asp Ala Ile Ile Lys Ile Glu Ser Phe Leu Asp
290 295
<210>167
<211>900
<212>DNA
<213>海洋原绿球藻
<400>167
atgcccgcct ctaagccgct tctgattgcg ctgctagggc ccacagcaag cggcaaaaca 60
tgcctggcct tacagctggc agaacagctg aagctgagcg tgctcaatgt ggattcccgc 120
cagctctaca tcggcatgga cgttggcacc gccaagccaa ccaaagaaca gcagcggcgt 180
gtgcagcatc acctgattga tcttcgtcgc ccagatcaac caatcaccct gcaggaattt 240
caggcagccg cgcaactgat ccttgcgcaa aagctaaggg aacaaaacat gccatttcta 300
gttggcggca gtggtcttta tctcaaagct ctcacttgtg ggctgcgccc acccgcagtg 360
ccaccccagc cagaactccg caagcagtta ggcgagctag gccaacgcac ctgccaccac 420
ctacttcaag ccgccgatcc caccgctgcc aaccgaattt cccctgctga tgccatgcgc 480
actcaaaggg gtctggaagt ggtttatgcc actggcaaac cgatcaccac tcaacaaggc 540
tcaagcccac ccccttggcg cgtgctggaa ctaggcctcg acccacacaa cctccgcgaa 600
cgcattggcc atcgcacaac acagctctat gccaatggtc tcatcgaaga aaccgaacac 660
ttgagtcatt gctacgacac cgatctgccc ttgctgcaaa cgattggcta tggggaagca 720
ctaaaggtga ttcaaggcct cctcaaccgt gaacaggcca tcgccctgac cacacgtcgt 780
actcaacagt tcgccaagcg tcagcgcaca tggttccgca gacaacatca cccctattgg 840
ctcaaaggtg aggaaccgct gagcgaagcg ctatcactga ttcaagcggg tctaaggtga 900
<210>168
<211>299
<212>PRT
<213>海洋原绿球藻
<400>168
Met Pro Ala Ser Lys Pro Leu Leu Ile Ala Leu Leu Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Cys Leu Ala Leu Gln Leu Ala Glu Gln Leu Lys Leu
20 25 30
Ser Val Leu Asn Val Asp Ser Arg Gln Leu Tyr Ile Gly Met Asp Val
35 40 45
Gly Thr Ala Lys Pro Thr Lys Glu Gln Gln Arg Arg Val Gln His His
50 55 60
Leu Ile Asp Leu Arg Arg Pro Asp Gln Pro Ile Thr Leu Gln Glu Phe
65 70 75 80
Gln Ala Ala Ala Gln Leu Ile Leu Ala Gln Lys Leu Arg Glu Gln Asn
85 90 95
Met Pro Phe Leu Val Gly Gly Ser Gly Leu Tyr Leu Lys Ala Leu Thr
100 105 110
Cys Gly Leu Arg Pro Pro Ala Val Pro Pro Gln Pro Glu Leu Arg Lys
115 120 125
Gln Leu Gly Glu Leu Gly Gln Arg Thr Cys His His Leu Leu Gln Ala
130 135 140
Ala Asp Pro Thr Ala Ala Asn Arg Ile Ser Pro Ala Asp Ala Met Arg
145 150 155 160
Thr Gln Arg Gly Leu Glu Val Val Tyr Ala Thr Gly Lys Pro Ile Thr
165 170 175
Thr Gln Gln Gly Ser Ser Pro Pro Pro Trp Arg Val Leu Glu Leu Gly
180 185 190
Leu Asp Pro His Asn Leu Arg Glu Arg Ile Gly His Arg Thr Thr Gln
195 200 205
Leu Tyr Ala Asn Gly Leu Ile Glu Glu Thr Glu His Leu Ser His Cys
210 215 220
Tyr Asp Thr Asp Leu Pro Leu Leu Gln Thr Ile Gly Tyr Gly Glu Ala
225 230 235 240
Leu Lys Val Ile Gln Gly Leu Leu Asn Arg Glu Gln Ala Ile Ala Leu
245 250 255
Thr Thr Arg Arg Thr Gln Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe
260 265 270
Arg Arg Gln His His Pro Tyr Trp Leu Lys Gly Glu Glu Pro Leu Ser
275 280 285
Glu Ala Leu Ser Leu Ile Gln Ala Gly Leu Arg
290 295
<210>169
<211>900
<212>DNA
<213>海洋原绿球藻
<400>169
atgcttcctt caaaaccact agtaatagtt ttaatcgggc cgacagcaag tggcaaaact 60
gagctagcaa tagatatcgc taaatatttt aatatccata tacacaatgt agattctaga 120
caaatttata gatttatgga catcggcaca gctaaaccaa cgaaagttca acaaagagct 180
ataaagcatt ttttaataga tgttgaagat ccctcagtaa aagtaaatgc aaagcaattt 240
caagaaattg ccacaaaatc aataaatcga gaattaaatc agaaaaaaac accctttcta 300
gttggtggta gcggattata tatgaattca ataataaagg gattttttgc accagatgtt 360
cctcctcaaa gttttctgag gtcccaattc gaaaaattag gtcaagaaaa atgttgggaa 420
cttctaaaag tttgtgaccc tgaattaaca aaaacaatta attatgccga tcaaataaga 480
accataagag gtttagaagt cttttatgta actggtaaaa gaatgtcatc tcaaagattc 540
caaaaccctc ctccatggag aatcttagaa ttgggtataa atagagtaga tttaaaggaa 600
agaattttca aaagaacaaa aaacatgttt gaatttggaa ttatagagga gaccaaaaat 660
atcattaatc aatatggatc gactttgcct ttactagaaa cgattggata taaagaggca 720
aaaaatgtaa tcaaagaaaa tttaacaatt gaagaagcta ttgaattaac aacaactaaa 780
acaatccaat ttgctaaaag acaaaaaaca tggttccgta ataagaacaa tgcaatatgg 840
cttaacaaca aaaacctact aaaagatgca ataattaaga tagagtatgc tttaggttaa 900
<210>170
<211>299
<212>PRT
<213>海洋原绿球藻
<400>170
Met Leu Pro Ser Lys Pro Leu Val Ile Val Leu Ile Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Glu Leu Ala Ile Asp Ile Ala Lys Tyr Phe Asn Ile
20 25 30
His Ile His Asn Val Asp Ser Arg Gln Ile Tyr Arg Phe Met Asp Ile
35 40 45
Gly Thr Ala Lys Pro Thr Lys Val Gln Gln Arg Ala Ile Lys His Phe
50 55 60
Leu Ilze Asp Val Glu Asp Pro Ser Val Lys Val Asn Ala Lys Gln Phe
65 70 75 80
Gln Glu Ile Ala Thr Lys Ser Ile Asn Arg Glu Leu Asn Gln Lys Lys
85 90 95
Thr Pro Phe Leu Val Gly Gly Ser Gly Leu Tyr Met Asn Ser Ile Ile
100 105 110
Lys Gly Phe Phe Ala Pro Asp Val Pro Pro Gln Ser Phe Leu Arg Ser
115 120 125
Gln Phe Glu Lys Leu Gly Gln Glu Lys Cys Trp Glu Leu Leu Lys Val
130 135 140
Cys Asp Pro Glu Leu Thr Lys Thr Ile Asn Tyr Ala Asp Gln Ile Arg
145 150 155 160
Thr Ile Arg Gly Leu Glu Val Phe Tyr Val Thr Gly Lys Arg Met Ser
165 170 175
Ser Gln Arg Phe Gln Asn Pro Pro Pro Trp Arg Ile Leu Glu Leu Gly
180 185 190
Ile Asn Arg Val Asp Leu Lys Glu Arg Ile Phe Lys Arg Thr Lys Asn
195 200 205
Met Phe Glu Phe Gly Ile Ile Glu Glu Thr Lys Asn Ile Ile Asn Gln
210 215 220
Tyr Gly Ser Thr Leu Pro Leu Leu Glu Thr Ile Gly Tyr Lys Glu Ala
225 230 235 240
Lys Asn Val Ile Lys Glu Asn Leu Thr Ile Glu Glu Ala Ile Glu Leu
245 250 255
Thr Thr Thr Lys Thr Ile Gln Phe Ala Lys Arg Gln Lys Thr Trp Phe
260 265 270
Arg Asn Lys Asn Asn Ala Ile Trp Leu Asn Asn Lys Asn Leu Leu Lys
275 280 285
Asp Ala Ile Ile Lys Ile Glu Tyr Ala Leu Gly
290 295
<210>171
<21l>900
<212>DNA
<213>海洋原绿球藻
<400>171
atgtctagag accttcctaa tgtcataatt ttgattgggc ctactgcaag tggcaaaaca 60
gaattagcta ttgaaattgc agaatatttt aaaactcata tacataatat cgattcaagg 120
caaatttata agtccatgga tattggaaca gccaagccat ctaaaaacca acaaaaaaaa 180
atcaagcatt ttttaataga tattgaggag ccaatccatc caattaatgt taaacaattt 240
caaggaattg ctcaaaaatc aataaaaagt gaaattaaac aaaataatct accttttctt 300
gttggaggta gtgggttgta tatgaactcg ataacaaaag gtttttttgt accagacgtc 360
ccacctcaaa atgatttgag aaaacaattg gaagaacttg gtcagaaaaa atgttgggac 420
ttattaaaaa attgtgatcc attatcaaca aaaaaaatta attttgctga ccatattaga 480
acaataagag ctttagaagt cttttacgtc acaggaaaac ctttgtcaac tttgaaagtg 540
caaagggcgc ctgactggag aatactagag cttggattag atagagataa tttaaaagaa 600
agaatattac aaaggacaaa aaatatgttt tcagcgggaa ttattgaaga gacaaactac 660
ctcatctcta aatacggatt tgatttgcca atattagaaa ccattggata tcgagaagct 720
aaggatgttt taaaaaacca ttcaaccatt gacaaagcaa ttgagttaac tacgacaaaa 780
actatccaat atgcaaaaag acaaaaaact tggtttcgta ataaaaataa tcctctttgg 840
cttgataaca aaaaccttct aaaagatgca ataattaaga tagagtcttt tttaagctaa 900
<210>172
<211>299
<212>PRT
<213>海洋原绿球藻
<400>172
Met Ser Arg Asp Leu Pro Asn Val Ile Ile Leu Ile Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Glu Leu Ala Ile Glu Ile Ala Glu Tyr Phe Lys Thr
20 25 30
His Ile His Asn Ile Asp Ser Arg Gln Ile Tyr Lys Ser Met Asp Ile
35 40 45
Gly Thr Ala Lys Pro Ser Lys Asn Gln Gln Lys Lys Ile Lys His Phe
50 55 60
Leu Ile Asp Ile Glu Glu Pro Ile His Pro Ile Asn Val Lys Gln Phe
65 70 75 80
Gln Gly Ile Ala Gln Lys Ser Ile Lys Ser Glu Ile Lys Gln Ash Ash
85 90 95
Leu Pro Phe Leu Val Gly Gly Ser Gly Leu Tyr Met Asn Ser Ile Thr
100 105 110
Lys Gly Phe Phe Val Pro Asp Val Pro Pro Gln Asn Asp Leu Arg Lys
115 120 125
Gln Leu Glu Glu Leu Gly Gln Lys Lys Cys Trp Asp Leu Leu Lys Asn
130 135 140
Cys Asp Pro Leu Ser Thr Lys Lys Ile Asn Phe Ala Asp His Ile Arg
145 150 155 160
Thr Ile Arg Ala Leu Glu Val Phe Tyr Val Thr Gly Lys Pro Leu Ser
165 170 175
Thr Leu Lys Val Gln Arg Ala Pro Asp Trp Arg Ile Leu Glu Leu Gly
180 185 190
Leu Asp Arg Asp Asn Leu Lys Glu Arg Ile Leu Gln Arg Thr Lys Asn
195 200 205
Met Phe Ser Ala Gly Ile Ile Glu Glu Thr Asn Tyr Leu Ile Ser Lys
210 215 220
Tyr Gly Phe Asp Leu Pro Ile Leu Glu Thr Ile Gly Tyr Arg Glu Ala
225 230 235 240
Lys Asp Val Leu Lys Asn His Ser Thr Ile Asp Lys Ala Ile Glu Leu
245 250 255
Thr Thr Thr Lys Thr Ile Gln Tyr Ala Lys Arg Gln Lys Thr Trp Phe
260 265 270
Arg Asn Lys Asn Asn Pro Leu Trp Leu Asp Asn Lys Asn Leu Leu Lys
275 280 285
Asp Ala Ile Ile Lys Ile Glu Ser Phe Leu Ser
290 295
<210>173
<211>900
<212>DNA
<213>海洋原绿球藻
<400>173
atgttccaac caaaaccatt agtaatagtt ttaatcggac ctacagcgag tggcaaaact 60
gagctaggaa tcgaaattgc taaatatttt aatctcaata tacataatgt cgactcaaga 120
caactttatc gatttatgga cataggtaca gcaaagccaa ctaaagaaca acaaaaaaca 180
ataaagcatt ttttaataga tcttgaagaa ccctctagcc aagtaaatgc aaaacaattt 240
caagaaattg ctaccaaatc aataaatcga gaactaaatc aaaacagaat tccttttcta 300
ataggcggga gcgggctata tatgaattca ataataaagg gtttctttgc tccgaatgtg 360
ccccctcaaa aagttttaag atcacaattc gaaaaactag ggcaagaaaa atgttgggaa 420
cttttaaaaa tttgtgatcc tgtcttaaca aaaaaaatca attacgctga tcaagttaga 480
acgataagag ctttagaagt cttttatgta acgggcaaac caatatcctc tcaaaaattc 540
cagaaccctc ccccatggaa aatattagaa ttaggtctat acagagaaga tttaaaagaa 600
cgtattttca aaagaacaaa aaatatgttt gaatttggaa ttatagatga gaccaaaaag 660
attattaatc aatatggatc aaatttacct ttactagaaa cgattggata tagagaagcc 720
aaggatgtaa taaaggaaaa tttaaaactt gaaaaagcta ttgaaataac tacaacaaaa 780
acaatccaat ttgctaagag acaaaaaaca tggttccgta ataaaaacaa tccaatatgg 840
cttaataaca aaaacctact aaaagatgca ataatcaata taaagcatgc attacgctag 900
<210>174
<211>299
<212>PRT
<213>海洋原绿球藻
<400>174
Met Phe Gln Pro Lys Pro Leu Val Ile Val Leu Ile Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Glu Leu Gly Ile Glu Ile Ala Lys Tyr Phe Asn Leu
20 25 30
Asn Ile His Asn Val Asp Ser Arg Gln Leu Tyr Arg Phe Met Asp Ile
35 40 45
Gly Thr Ala Lys Pro Thr Lys Glu Gln Gln Lys Thr Ile Lys His Phe
50 55 60
Leu Ile Asp Leu Glu Glu Pro Ser Ser Gln Val Asn Ala Lys Gln Phe
65 70 75 80
Gln Glu Ile Ala Thr Lys Ser Ile Asn Arg Glu Leu Asn Gln Asn Arg
85 90 95
Ile Pro Phe Leu Ile Gly Gly Ser Gly Leu Tyr Met Asn Ser Ile Ile
100 105 110
Lys Gly Phe Phe Ala Pro Asn Val Pro Pro Gln Lys Val Leu Arg Ser
115 120 125
Gln Phe Glu Lys Leu Gly Gln Glu Lys Cys Trp Glu Leu Leu Lys Ile
130 135 140
Cys Asp Pro Val Leu Thr Lys Lys Ile Asn Tyr Ala Asp Gln Val Arg
145 150 155 160
Thr Ile Arg Ala Leu Glu Val Phe Tyr Val Thr Gly Lys Pro Ile Ser
165 170 175
Ser Gln Lys Phe Gln Asn Pro Pro Pro Trp Lys Ile Leu Glu Leu Gly
180 185 190
Leu Tyr Arg Glu Asp Leu Lys Glu Arg Ile Phe Lys Arg Thr Lys Asn
195 200 205
Met Phe Glu Phe Gly Ile Ile Asp Glu Thr Lys Lys Ile Ile Asn Gln
210 215 220
Tyr Gly Ser Asn Leu Pro Leu Leu Glu Thr Ile Gly Tyr Arg Glu Ala
225 230 235 240
Lys Asp Val Ile Lys Glu Asn Leu Lys Leu Glu Lys Ala Ile Glu Ile
245 250 255
Thr Thr Thr Lys Thr Ile Gln Phe Ala Lys Arg Gln Lys Thr Trp Phe
260 265 270
Arg Asn Lys Asn Asn Pro Ile Trp Leu Asn Asn Lys Asn Leu Leu Lys
275 280 285
Asp Ala Ile Ile Asn Ile Lys His Ala Leu Arg
290 295
<210>175
<211>900
<212>DNA
<213>海洋原绿球藻
<400>175
atgcaaccca ataagcctct tgtcatagcc cttatgggcc caactgcaag tggtaaaact 60
gaacttgcaa ttgatattgc aaaaaaaatc aattctaata ttcacaatat cgattctcgc 120
caaatctata ttgatatgga tattggcacc gcaaagccaa cagcagttca acaaagccaa 180
gtaaaccatt tccttattga tgtttgcttg ccatctaaac caatcaatct tcatgatttt 240
caatccatag cccaaacatc cattgagaga gatttagaaa aaaagggttt aacgttactt 300
gtaggaggaa gtggcttata tcttcaagcc ttaattggag gacttaatcc tccagctgta 360
cctcctcaga aattcttaag agatcaactt aaaaaaatcg ataaaaccga actacacaaa 420
ttattaaagc tttgtgaccc tttttcagcg cagagaatac atccagaaga ctcaataaga 480
atcattcgtg ctcttgaagt tttttatgcc acgggaaaaa tgttttcgaa ggaaaaaaac 540
atgacgccta ttccttggag agtgttagaa cttggagtaa atcctgaaaa tttaatcagt 600
agaattcaac gtagaactga agaaatgtat aaaaaaggtt taatagaaga aacagaagat 660
ttgatcaata aatacggaaa aaatttacag ttgcttaaaa ctattggata tggtgaagca 720
aggtctatga ttaatggaaa gattaattat gaggaggctt tagaaataac aatcaaacga 780
acttgccaat tagccaaaag acaaaaaact tggtttagaa ataagcatca ttctaaatgg 840
ctaaataatg agaatgcatt acccgaagct ttaacttcta tctatgagtt tctaggttga 900
<210>176
<211>299
<212>PRT
<213>海洋原绿球藻
<400>176
Met Gln Pro Asn Lys Pro Leu Val Ile Ala Leu Met Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Glu Leu Ala Ile Asp Ile Ala Lys Lys Ile Asn Ser
20 25 30
Asn Ile His Asn Ile Asp Ser Arg Gln Ile Tyr Ile Asp Met Asp Ile
35 40 45
Gly Thr Ala Lys Pro Thr Ala Val Gln Gln Ser Gln Val Asn His Phe
50 55 60
Leu Ile Asp Val Cys Leu Pro Ser Lys Pro Ile Asn Leu His Asp Phe
65 70 75 80
Gln Ser Ile Ala Gln Thr Ser Ile Glu Arg Asp Leu Glu Lys Lys Gly
85 90 95
Leu Thr Leu Leu Val Gly Gly Ser Gly Leu Tyr Leu Gln Ala Leu Ile
100 105 110
Gly Gly Leu Asn Pro Pro Ala Val Pro Pro Gln Lys Phe Leu Arg Asp
115 120 125
Gln Leu Lys Lys Ile Asp Lys Thr Glu Leu His Lys Leu Leu Lys Leu
130 135 140
Cys Asp Pro Phe Ser Ala Gln Arg Ile His Pro Glu Asp Ser Ile Arg
145 150 155 160
Ile Ile Arg Ala Leu Glu Val Phe Tyr Ala Thr Gly Lys Met Phe Ser
165 170 175
Lys Glu Lys Asn Met Thr Pro Ile Pro Trp Arg Val Leu Glu Leu Gly
180 185 190
Val Asn Pro Glu Asn Leu Ile Ser Arg Ile Gln Arg Arg Thr Glu Glu
195 200 205
Met Tyr Lys Lys Gly Leu Ile Glu Glu Thr Glu Asp Leu Ile Asn Lys
210 215 220
Tyr Gly Lys Asn Leu Gln Leu Leu Lys Thr Ile Gly Tyr Gly Glu Ala
225 230 235 240
Arg Ser Met Ile Asn Gly Lys Ile Asn Tyr Glu Glu Ala Leu Glu Ile
245 250 255
Thr Ile Lys Arg Thr Cys Gln Leu Ala Lys Arg Gln Lys Thr Trp Phe
260 265 270
Arg Asn Lys His His Ser Lys Trp Leu Asn Asn Glu Asn Ala Leu Pro
275 280 285
Glu Ala Leu Thr Ser Ile Tyr Glu Phe Leu Gly
290 295
<210>177
<211>900
<212>DNA
<213>海洋原绿球藻
<400>177
atgcaaccca ataaacctct tgtcgtagcc cttatgggcc caactgcaag tggtaaaact 60
gaacttgcga ttgatattgc aaaaaaaatc aattctaata ttcacaatat tgattctcgc 120
caaatttata ttgatatgga tattggcacc gcaaagccaa cagcagttca acaaagccaa 180
gtaaaccatt tccttattga tatttgcttg ccatctaaac caatcaatct tcatgatttt 240
caatccatag ccaaaacatc cattgagaga gatttagaaa aaaagggttt aactttactt 300
gtaggaggaa gtggtttata tctgcaagcc ttaattggag gacttaatcc tccagcggta 360
cctcctcaaa aattcttaag agatcaactt aaaaaaatcg ataaagccga acgacacaaa 420
ttattaaagc tttgtgaccc tttctcagcg cagagaatac atccagaaga ctcaataaga 480
ataattcgtg ctcttgaagt tttttatgca acaggaagaa tgttttcgaa ggctaaaaac 540
atgaggccta ctccttggag agttttggaa cttggattaa atcctgaaaa tttaaccagt 600
agaattcaac ttagaactag agaaatgtat aaaaaagggt tagtagaaga aacaggagat 660
ttgatcaata aatacggaaa taatttacag ttgcttaaaa ctattggata tggtgaagca 720
aggtctatga ttaatggaaa gattaattat gaggaggctt tagaaataac aataaaacga 780
acttgccaat tagccaaaag acaaaaaact tggtttagaa ataagcataa ttctaaatgg 840
ctaaatgatg agaatgcatt acccgaagct ttagcttcta tctatgagtt tcttggttga 900
<210>178
<211>299
<212>PRT
<213>海洋原绿球藻
<400>178
Met Gln Pro Asn Lys Pro Leu Val Val Ala Leu Met Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Glu Leu Ala Ile Asp Ile Ala Lys Lys Ile Ash Ser
20 25 30
Asn Ile His Asn Ile Asp Ser Arg Gln Ile Tyr Ile Asp Met Asp Ile
35 40 45
Gly Thr Ala Lys Pro Thr Ala Val Gln Gln Ser Gln Val Asn His Phe
50 55 60
Leu Ile Asp Ile Cys Leu Pro Ser Lys Pro Ile Asn Leu His Asp Phe
65 70 75 80
Gln Ser Ile Ala Lys Thr Ser Ile Glu Arg Asp Leu Glu Lys Lys Gly
85 90 95
Leu Thr Leu Leu Val Gly GIy Ser Gly Leu Tyr Leu Gln Ala Leu Ile
100 105 110
Gly Gly Leu Asn Pro Pro Ala Val Pro Pro Gln Lys Phe Leu Arg Asp
115 120 125
Gln Leu Lys Lys Ile Asp Lys Ala Glu Arg His Lys Leu Leu Lys Leu
130 135 140
Cys Asp Pro Phe Ser Ala Gln Arg Ile His Pro Glu Asp Ser Ile Arg
145 150 155 160
Ile Ile Arg Ala Leu Glu Val Phe Tyr Ala Thr Gly Arg Met Phe Ser
165 170 175
Lys Ala Lys Asn Met Arg Pro Thr Pro Trp Arg Val Leu Glu Leu Gly
180 185 190
Leu Asn Pro Glu Asn Leu Thr Ser Arg Ile Gln Leu Arg Thr Arg Glu
195 200 205
Met Tyr Lys Lys Gly Leu Val Glu Glu Thr Gly Asp Leu Ile Asn Lys
210 215 220
Tyr Gly Asn Asn Leu Gln Leu Leu Lys Thr Ile Gly Tyr Gly Glu Ala
225 230 235 240
Arg Ser Met Ile Asn Gly Lys Ile Asn Tyr Glu Glu Ala Leu Glu Ile
245 250 255
Thr Ile Lys Arg Thr Cys Gln Leu Ala Lys Arg Gln Lys Thr Trp Phe
260 265 270
Arg Asn Lys His Asn Ser Lys Trp Leu Asn Asp Glu Asn Ala Leu Pro
275 280 285
Glu Ala Leu Ala Ser Ile Tyr Glu Phe Leu Gly
290 295
<210>179
<211>1023
<212>DNA
<213>聚球藻属物种
<400>179
atggatcaaa accggtctcc aaatgggcgc gactgtcgag aacctccctc cccctcctcg 60
actgcaaggc cggggcttgt cgtcatcgct ggccccacgg ccaccggcaa gtcgcgccag 120
gcgctgctgt tggcccagcg gctgggatcc cccctgttga acgccgactc tcgtcaggtg 180
taccgggagt tcgacatcgg cactgccaag cccactcccg ccgagcgggc cctctggccc 240
cacgagttga ttgactttgt ggatccccgc cacacctata cagtggccga gtttcagcag 300
gcggcccagg cgcgcattgc cgcagcccat cggcagggac agactcctat tctggtgggg 360
ggcacagggc tctacattca gtcgatcacg gcaggcttag ggatcccggc agttcccccc 420
cagcctcagt tgcgcgctca actggagacg tggccgcccg agatccgcta cgcctggctg 480
cagcagttgg atcctgtggc agcggggcag attcaccctc atgatgaagt gcgcaccctg 540
cgggctttgg agatcatcta caccactggg aagccggctt cctacctgcg gcaggcccat 600
cctcctgact accccatttt gctcttgggg ttgcactgcc caatgccccg tctggaacaa 660
cgcattgccc ggcgcacagc agaaatgctg gcggcaggct ggattgagga ggtaaaaacg 720
ctgcggcagc gctacggccc agacctgccc ctcttgcaga ccttgggcta tgcggagatc 780
ggcgcttacc tggaagggcg gatcccggca gccgagctgc agcctctgat tgtgcggcgc 840
acccgccagt ttgccaagcg gcagatgacc tggtttcggg ccatgccagg gatccagtgg 900
ctggactgcg aggccgagga tctgcccgaa cagatctgga agcgggttac agcgtggatg 960
gcagctcaga ccagcgccgg aaccactcct gccgtcgccg cgcgtccgcc cgccgatccg 1020
taa 1023
<210>180
<211>340
<212>PRT
<213>聚球藻属物种
<400>180
Met Asp Gln Asn Arg Ser Pro Asn Gly Arg Asp Cys Arg Glu Pro Pro
1 5 10 15
Ser Pro Ser Ser Thr Ala Arg Pro Gly Leu Val Val Ile Ala Gly Pro
20 25 30
Thr Ala Thr Gly Lys Ser Arg Gln Ala Leu Leu Leu Ala Gln Arg Leu
35 40 45
Gly Ser Pro Leu Leu Asn Ala Asp Ser Arg Gln Val Tyr Arg Glu Phe
50 55 60
Asp Ile Gly Thr Ala Lys Pro Thr Pro Ala Glu Arg Ala Leu Trp Pro
65 70 75 80
His Glu Leu Ile Asp Phe Val Asp Pro Arg His Thr Tyr Thr Val Ala
85 90 95
Glu Phe Gln Gln Ala Ala Gln Ala Arg Ile Ala Ala Ala His Arg Gln
100 105 110
Gly Gln Thr Pro Ile Leu Val Gly Gly Thr Gly Leu Tyr Ile Gln Ser
115 120 125
Ile Thr Ala Gly Leu Gly Ile Pro Ala Val Pro Pro Gln Pro Gln Leu
130 135 140
Arg Ala Gln Leu Glu Thr Trp Pro Pro Glu Ile Arg Tyr Ala Trp Leu
145 150 155 160
Gln Gln Leu Asp Pro Val Ala Ala Gly Gln Ile His Pro His Asp Glu
165 170 175
Val Arg Thr Leu Arg Ala Leu Glu Ile Ile Tyr Thr Thr Gly Lys Pro
180 185 190
Ala Ser Tyr Leu Arg Gln Ala His Pro Pro Asp Tyr Pro Ile Leu Leu
195 200 205
Leu Gly Leu His Cys Pro Met Pro Arg Leu Glu Gln Arg Ile Ala Arg
210 215 220
Arg Thr Ala Glu Met Leu Ala Ala Gly Trp Ile Glu Glu Val Lys Thr
225 230 235 240
Leu Arg Gln Arg Tyr Gly Pro Asp Leu Pro Leu Leu Gln Thr Leu Gly
245 250 255
Tyr Ala Glu Ile Gly Ala Tyr Leu Glu Gly Arg Ile Pro Ala Ala Glu
260 265 270
Leu Gln Pro Leu Ile Val Arg Arg Thr Arg Gln Phe Ala Lys Arg Gln
275 280 285
Met Thr Trp Phe Arg Ala Met Pro Gly Ile Gln Trp Leu Asp Cys Glu
290 295 300
Ala Glu Asp Leu Pro Glu Gln Ile Trp Lys Arg Val Thr Ala Trp Met
305 310 315 320
Ala Ala Gln Thr Ser Ala Gly Thr Thr Pro Ala Val Ala Ala Arg Pro
325 330 335
Pro Ala Asp Pro
340
<210>181
<211>891
<212>DNA
<213>集胞藻属物种(Synechocystis sp.)
<400>181
atgaccccat taatggtgct ggttttgggc ccaaccgcca gcggcaagac cagccttggc 60
attgccctgg cccaacagct cgattgccgg gttctctcga ttgattcgcg ccagctctat 120
gcaggcatgg acatcggcac ggccaaaccc acgcgcgacg agcagcagca ggcccgccac 180
gagctgttga acctgagcac gcccgatcag cccatcaacc tgcagcagtt ctgctcccat 240
gcccagacgc tgattgagca agagcaacag cgggggcggc ccgcgttgct ggtgggcggc 300
agcgggctgt atttgcaagc cttgagccaa gggctgcagc cccccgccct accgccacaa 360
acggggctgc gtcagcaact gcagcaactg gggcagtcct gttgtcatca gctgctgagc 420
caggcggacc ctcaagccgc agccaagatc gagcccaacg atccagtccg cacacagcgg 480
gcactggagg tgctctacgg aaccggtcaa accatctcaa gccaacaggg gcgctgcccg 540
ccggcctgcc gggtgctgga actggggctc aaccccagcg acctcaaaga gcgcattgag 600
cagcgcacag cctccctcta cgccaggggc ctcgtggccg aaaccgaaac gctcagcagg 660
cgctacggag ctgatctgcc ccttctgcag accattggtt acggcgaagc cctggcggta 720
ctcgccggca ggctcagcga gtccgaagcg caagcactca ccagccggcg cacctggctg 780
tttgccaaac gccaacgcac ctggttccgc aaccgccatc agcccctttg gctaaacacg 840
gaatccgcac ttgaagaagc attggaagcc attgcagcgg ccaggtctta g 891
<210>182
<211>296
<212>PRT
<213>集胞藻属物种
<400>182
Met Thr Pro Leu Met Val Leu Val Leu Gly Pro Thr Ala Ser Gly Lys
1 5 10 15
Thr Ser Leu Gly Ile Ala Leu Ala Gln Gln Leu Asp Cys Arg Val Leu
20 25 30
Ser Ile Asp Ser Arg Gln Leu Tyr Ala Gly Met Asp Ile Gly Thr Ala
35 40 45
Lys Pro Thr Arg Asp Glu Gln Gln Gln Ala Arg His Glu Leu Leu Asn
50 55 60
Leu Ser Thr Pro Asp Gln Pro Ile Asn Leu Gln Gln Phe Cys Ser His
65 70 75 80
Ala Gln Thr Leu Ile Glu Gln Glu Gln Gln Arg Gly Arg Pro Ala Leu
85 90 95
Leu Val Gly Gly Ser Gly Leu Tyr Leu Gln Ala Leu Ser Gln Gly Leu
100 105 110
Gln Pro Pro Ala Leu Pro Pro Gln Thr Gly Leu Arg Gln Gln Leu Gln
115 120 125
Gln Leu Gly Gln Ser Cys Cys His Gln Leu Leu Ser Gln Ala Asp Pro
130 135 140
Gln Ala Ala Ala Lys Ile Glu Pro Asn Asp Pro Val Arg Thr Gln Arg
145 150 155 160
Ala Leu Glu Val Leu Tyr Gly Thr Gly Gln Thr Ile Ser Ser Gln Gln
165 170 175
Gly Arg Cys Pro Pro Ala Cys Arg Val Leu Glu Leu Gly Leu Asn Pro
180 185 190
Ser Asp Leu Lys Glu Arg Ile Glu Gln Arg Thr Ala Ser Leu Tyr Ala
195 200 205
Arg Gly Leu Val Ala Glu Thr Glu Thr Leu Ser Arg Arg Tyr Gly Ala
210 215 220
Asp Leu Pro Leu Leu Gln Thr Ile Gly Tyr Gly Glu Ala Leu Ala Val
225 230 235 240
Leu Ala Gly Arg Leu Ser Glu Ser Glu Ala Gln Ala Leu Thr Ser Arg
245 250 255
Arg Thr Trp Leu Phe Ala Lys Arg Gln Arg Thr Trp Phe Arg Asn Arg
260 265 270
His Gln Pro Leu Trp Leu Asn Thr Glu Ser Ala Leu Glu Glu Ala Leu
275 280 285
Glu Ala Ile Ala Ala Ala Arg Ser
290 295
<210>183
<211>912
<212>DNA
<213>集胞藻属物种
<400>183
atggccaaag ttccgcccct gattgttatt tgtggcacca ccgccagtgg gaaatcccaa 60
ctagcccttg atctagccca gcggttaaac gcagttattt tgggggccga ttccaggcaa 120
atttataaag aattggacat tggcaccgct aaacccaccc ttggcgatcg ccaaacggta 180
ccccattatc tgattgacat ttgtgaacca acggaaaact tcaccctagc ggaataccaa 240
cggcaagccc aggagttgat tgcgtcatta aaccaaccga ttctgctggt aggcggcact 300
ggtttataca tccaggcgat cgtcaagggg ttaaaaattc ctgctgttcc accccagacc 360
aatttacgtg aacagttagc taacctcggc caaccctttt gttatcaact tttaagtcaa 420
gtggaccccg tagcccaaag caaaattgaa cccgctgacg tggtgcggac cctgcgggct 480
ttggaagttt tctatgccac tgggcgaccc atttccagcc tacaagggga aaatcccccc 540
agttatccca ttgtgcaaat cggtttaggt ctggaaccgg agcaactcca acccagaatt 600
gttcaccgta cccatgccat ggttgaggcg gggttagtga aggaagttga gggtttaatc 660
aatcagtatg gggaagacct acccctgctc catacccttg gttacgctga gattaaacaa 720
tatctccaag gtcaaatatc gttaactcaa gcgacggagt ccatcattgt ccacaccagg 780
caatttgcca aacgtcagcg cacttggttc cgcaaagatt cggcaatcca ctggtttgat 840
gctaaccagc caaacctatt agattcggtg acaaagttag tacaagttga cgtaaacgaa 900
ggaatgtttt aa 912
<210>184
<211>303
<212>PRT
<213>集胞藻属物种
<400>184
Met Ala Lys Val Pro Pro Leu Ile Val Ile Cys Gly Thr Thr Ala Ser
1 5 10 15
Gly Lys Ser Gln Leu Ala Leu Asp Leu Ala Gln Arg Leu Asn Ala Val
20 25 30
Ile Leu Gly Ala Asp Ser Arg Gln Ile Tyr Lys Glu Leu Asp Ile Gly
35 40 45
Thr Ala Lys Pro Thr Leu Gly Asp Arg Gln Thr Val Pro His Tyr Leu
50 55 60
Ile Asp Ile Cys Glu Pro Thr Glu Asn Phe Thr Leu Ala Glu Tyr Gln
65 70 75 80
Arg Gln Ala Gln Glu Leu Ile Ala Ser Leu Asn Gln Pro Ile Leu Leu
85 90 95
Val Gly Gly Thr Gly Leu Tyr Ile Gln Ala Ile Val Lys Gly Leu Lys
100 105 110
Ile Pro Ala Val Pro Pro Gln Thr Asn Leu Arg Glu Gln Leu Ala Asn
115 120 125
Leu Gly Gln Pro Phe Cys Tyr Gln Leu Leu Ser Gln Val Asp Pro Val
130 135 140
Ala Gln Ser Lys Ile Glu Pro Ala Asp Val Val Arg Thr Leu Arg Ala
145 150 155 160
Leu Glu Val Phe Tyr Ala Thr Gly Arg Pro Ile Ser Ser Leu Gln Gly
165 170 175
Glu Asn Pro Pro Ser Tyr Pro Ile Val Gln Ile Gly Leu Gly Leu Glu
180 185 190
Pro Glu Gln Leu Gln Pro Arg Ile Val His Arg Thr His Ala Met Val
195 200 205
Glu Ala Gly Leu Val Lys Glu Val Glu Gly Leu Ile Asn Gln Tyr Gly
210 215 220
Glu Asp Leu Pro Leu Leu His Thr Leu Gly Tyr Ala Glu Ile Lys Gln
225 230 235 240
Tyr Leu Gln Gly Gln Ile Ser Leu Thr Gln Ala Thr Glu Ser Ile Ile
245 250 255
Val His Thr Arg Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe Arg Lys
260 265 270
Asp Ser Ala Ile His Trp Phe Asp Ala Asn Gln Pro Asn Leu Leu Asp
275 280 285
Ser Val Thr Lys Leu Val Gln Val Asp Val Asn Glu Gly Met Phe
290 295 300
<210>185
<211>933
<212>DNA
<213>聚球藻属物种
<400>185
atgcctcaca ccagccccga aacggaggcc caacgcgaca gcaacgcccc cctcgtggtg 60
gtgctgctcg gacccaccgc cagcggcaaa accgccctcg cgctggagct ggccgagcgc 120
ttcgacctgg agatcatcaa tgtggattcc cggcagctgt accaggagat gagcgtaggc 180
acggccaagc ccagcccgga gcaacaggcc cgcatccgcc accatctgct cgacctgcgc 240
ccccccgacc aaccgatcac cttgcaggaa ttccaggagg aagccctcca ggcggtgaat 300
cagagcctgg ccaaacgcgg cgccgccttt ctcgtgggcg gcagcgggct ctatctcaag 360
gccctcacag ctggtctgcg cccccccgct gtggcgcccc aaccggcgct gcgccggcaa 420
ctggcgcagc tgggccaacc gctgtgccac cagctgctca acactgccga tcccgaggca 480
gccgtgcgca tcgccagcgc agatgcgctg cgcacccaac gcgccctgga ggtgttgtac 540
gccacaggcg caccgatgag ccgccaaacc agtgccagtc cgccgccctg gcgcgtgctg 600
gagctgggcc tcaatccgct cgacctgcgc cagcgcatca gtgcacgcac ccaggcgctg 660
tacagccaag ggctggtgga ggaaacccgc cagctgcgcg agcgctacgg ccccgagctg 720
ccgctcctgc aaaccattgg ctacggcgaa gcgttgcaag tgctcgcagg cgatctcagc 780
cgtcccgctg cgatcgccca caccacccgg cgcacccagc aattcgccaa acggcagcgc 840
acctggttcc ggcggcagca ccagccgcac tggctgcccg atgacaatcc cctgaatgaa 900
gccggacgcc tgatcgaagc gggtctaggc tga 933
<210>186
<211>310
<212>PRT
<213>聚球藻属物种
<400>186
Met Pro His Thr Ser Pro Glu Thr Glu Ala Gln Arg Asp Ser Asn Ala
1 5 10 15
Pro Leu Val Val Val Leu Leu Gly Pro Thr Ala Ser Gly Lys Thr Ala
20 25 30
Leu Ala Leu Glu Leu Ala Glu Arg Phe Asp Leu Glu Ile Ile Asn Val
35 40 45
Asp Ser Arg Gln Leu Tyr Gln Glu Met Ser Val Gly Thr Ala Lys Pro
50 55 60
Ser Pro Glu Gln Gln Ala Arg Ile Arg His His Leu Leu Asp Leu Arg
65 70 75 80
Pro Pro Asp Gln Pro Ile Thr Leu Gln Glu Phe Gln Glu Glu Ala Leu
85 90 95
Gln Ala Val Asn Gln Ser Leu Ala Lys Arg Gly Ala Ala Phe Leu Val
100 105 110
Gly Gly Ser Gly Leu Tyr Leu Lys Ala Leu Thr Ala Gly Leu Arg Pro
115 120 125
Pro Ala Val Ala Pro Gln Pro Ala Leu Arg Arg Gln Leu Ala Gln Leu
130 135 140
Gly Gln Pro Leu Cys His Gln Leu Leu Asn Thr Ala Asp Pro Glu Ala
145 150 155 160
Ala Val Arg Ile Ala Ser Ala Asp Ala Leu Arg Thr Gln Arg Ala Leu
165 170 175
Glu Val Leu Tyr Ala Thr Gly Ala Pro Met Ser Arg Gln Thr Ser Ala
180 185 190
Ser Pro Pro Pro Trp Arg Val Leu Glu Leu Gly Leu Asn Pro Leu Asp
195 200 205
Leu Arg Gln Arg Ile Ser Ala Arg Thr Gln Ala Leu Tyr Ser Gln Gly
210 215 220
Leu Val Glu Glu Thr Arg Gln Leu Arg Glu Arg Tyr Gly Pro Glu Leu
225 230 235 240
Pro Leu Leu Gln Thr Ile Gly Tyr Gly Glu Ala Leu Gln Val Leu Ala
245 250 255
Gly Asp Leu Ser Arg Pro Ala Ala Ile Ala His Thr Thr Arg Arg Thr
260 265 270
Gln Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe Arg Arg Gln His Gln
275 280 285
Pro His Trp Leu Pro Asp Asp Asn Pro Leu Asn Glu Ala Gly Arg Leu
290 295 300
Ile Glu Ala Gly Leu Gly
305 310
<210>187
<211>900
<212>DNA
<213>聚球藻属物种
<400>187
ttgacgcagc cagaaccctt ggtgatcacg ttgctggggc ccaccgccag cggcaaaaca 60
gccctgtcgc tggagatcgc tgaacggctc aatctgccgg tgatcaacgt ggattccagg 120
cagctgtacc gggagatgac cgtgggcacc gccaaaccga cggcggagca acgggcgcgg 180
gtgccacacc atttgctgga tctacgcaac cccgatcagc cgatcaccct gcaggaattt 240
caggcggagg ccgagccctg catccagcgt gagctgcaaa gcaggggcat ggcactgctg 300
gtggggggta gtggcttgta tctcaaggcc ctcaccagtg gcctcaaacc accggcggtg 360
ccaccgcagg cgcagctgcg tgagcagctg agccaattgg gtcaagcgat ctgccatccg 420
ctgctccagc aggccgatcc aacggccggc gccaagatcg cccccgctga tgccgtacgc 480
acccaacggg ctctggaggt gctgtacgcc accggtcgac cgatgagcag ccaggccaca 540
gctacaccac cgccctggcg ggttttggaa ctggggctag atcctgccaa cctgcgccag 600
cgcattcagc agcgcaccga ccagctctac agcgacgggt tggtggagga gacccggcaa 660
ctttcggagc gctacggcgc tgatctgcca ttgctgcaga ccattggcta cggcgaggcc 720
ctgcagctcc tcgaaggaac gatgaaccag gcgaaagcca accgaatcac cacccagcgc 780
acgcggcaat tcgccaagcg tcagcgcacc tggttccggc gccagcacca gccccattgg 840
ctggcgccgg ctacagaact tgatcaggcg atgacgttga tcgagcagca tctaaggtga 900
<210>188
<211>299
<212>PRT
<213>聚球藻属物种
<400>188
Met Thr Gln Pro Glu Pro Leu Val Ile Thr Leu Leu Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Ala Leu Ser Leu Glu Ile Ala Glu Arg Leu Asn Leu
20 25 30
Pro Val Ile Asn Val Asp Ser Arg Gln Leu Tyr Arg Glu Met Thr Val
35 40 45
Gly Thr Ala Lys Pro Thr Ala Glu Gln Arg Ala Arg Val Pro His His
50 55 60
Leu Leu Asp Leu Arg Asn Pro Asp Gln Pro Ile Thr Leu Gln Glu Phe
65 70 75 80
Gln Ala Glu Ala Glu Pro Cys Ile Gln Arg Glu Leu Gln Ser Arg Gly
85 90 95
Met Ala Leu Leu Val Gly Gly Ser Gly Leu Tyr Leu Lys Ala Leu Thr
100 105 110
Ser Gly Leu Lys Pro Pro Ala Val Pro Pro Gln Ala Gln Leu Arg Glu
115 120 125
Gln Leu Ser Gln Leu Gly Gln Ala Ile Cys His Pro Leu Leu Gln Gln
130 135 140
Ala Asp Pro Thr Ala Gly Ala Lys Ile Ala Pro Ala Asp Ala Val Arg
145 150 155 160
Thr Gln Arg Ala Leu Glu Val Leu Tyr Ala Thr Gly Arg Pro Met Ser
165 170 175
Ser Gln Ala Thr Ala Thr Pro Pro Pro Trp Arg Val Leu Glu Leu Gly
180 185 190
Leu Asp Pro Ala Asn Leu Arg Gln Arg Ile Gln Gln Arg Thr Asp Gln
195 200 205
Leu Tyr Ser Asp Gly Leu Val Glu Glu Thr Arg Gln Leu Ser Glu Arg
210 215 220
Tyr Gly Ala Asp Leu Pro Leu Leu Gln Thr Ile Gly Tyr Gly Glu Ala
225 230 235 240
Leu Gln Leu Leu Glu Gly Thr Met Asn Gln Ala Lys Ala Asn Arg Ile
245 250 255
Thr Thr Gln Arg Thr Arg Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe
260 265 270
Arg Arg Gln His Gln Pro His Trp Leu Ala Pro Ala Thr Glu Leu Asp
275 280 285
Gln Ala Met Thr Leu Ile Glu Gln His Leu Arg
290 295
<210>189
<211>933
<212>DNA
<213>聚球藻属物种
<400>189
atgaatcaga tcaactctga atttggcccc gatgccggtg caaaggcacc cctcgttgtg 60
gcgctcgttg ggccaacggc gagcggcaaa accgcgctcg cgcttgagct tgctgagcac 120
ttccaactgg agatcctcaa catcgattct cgccagcttt accgagagat ggatatcggc 180
acggccaaac ccacagccga gcaacaacag cgcgtcaccc atcacctcct cgacctgcgc 240
tcccctgatc aaccgatcac gcttcaggaa tttcagcaag aagccactgc ggccgtgagc 300
caggttctca aagagcgagg cgttgctttt ttagctggag gcagcggctt gtacctcaag 360
gctctcaccc aaggactgca gcctccagcc gtgcctcctc aagccgagct tcgccgccag 420
ctgagctcac tcggtcaagc gaactgccac cagctcctcc aacaggccga tccccaagca 480
gccgccaaga tcgcccctgc cgatgcggtt cgcacccaac gtgctctgga ggtgctgtac 540
tccagcggca aaccgatgag cgcgcaacag tcgaccaatc ccccgccctg gcgcgtactg 600
gagctcggac tcaatcccat ggagctgcgc tcacgcatcg cccaacggac tctccagatt 660
tatcaggaag gcttgcttga agaaacgcgg cagctgagcc agcgctatgg gcctgacctc 720
cccatgctgc aaacgatcgg ctacggcgaa gcactagagg tgctgcaagg aggcctaagc 780
gaagctcagg ctatcgctac caccacccgc cgcacccagc agtttgccaa gcgtcaacgc 840
acctggttcc gccgccaaca cagtccccac tggctcacag gtcaggatgc cctcagcgaa 900
gcgatacgtc tgatcgaagc gggtctaggc tga 933
<210>190
<211>310
<212>PRT
<213>聚球藻属物种
<400>190
Met Asn Gln Ile Asn Ser Glu Phe Gly Pro Asp Ala Gly Ala Lys Ala
1 5 10 15
Pro Leu Val Val Ala Leu Val Gly Pro Thr Ala Ser Gly Lys Thr Ala
20 25 30
Leu Ala Leu Glu Leu Ala Glu His Phe Gln Leu Glu Ile Leu Asn Ile
35 40 45
Asp Ser Arg Gln Leu Tyr Arg Glu Met Asp Ile Gly Thr Ala Lys Pro
50 55 60
Thr Ala Glu Gln Gln Gln Arg Val Thr His His Leu Leu Asp Leu Arg
65 70 75 80
Ser Pro Asp Gln Pro Ile Thr Leu Gln Glu Phe Gln Gln Glu Ala Thr
85 90 95
Ala Ala Val Ser Gln Val Leu Lys Glu Arg Gly Val Ala Phe Leu Ala
100 105 110
Gly Gly Ser Gly Leu Tyr Leu Lys Ala Leu Thr Gln Gly Leu Gln Pro
115 120 125
Pro Ala Val Pro Pro Gln Ala Glu Leu Arg Arg Gln Leu Ser Ser Leu
130 135 140
Gly Gln Ala Asn Cys His Gln Leu Leu Gln Gln Ala Asp Pro Gln Ala
145 150 155 160
Ala Ala Lys Ile Ala Pro Ala Asp Ala Val Arg Thr Gln Arg Ala Leu
165 170 175
Glu Val Leu Tyr Ser Ser Gly Lys Pro Met Ser Ala Gln Gln Ser Thr
180 185 190
Asn Pro Pro Pro Trp Arg Val Leu Glu Leu Gly Leu Asn Pro Met Glu
195 200 205
Leu Arg Ser Arg Ile Ala Gln Arg Thr Leu Gln Ile Tyr Gln Glu Gly
210 215 220
Leu Leu Glu Glu Thr Arg Gln Leu Ser Gln Arg Tyr Gly Pro Asp Leu
225 230 235 240
Pro Met Leu Gln Thr Ile Gly Tyr Gly Glu Ala Leu Glu Val Leu Gln
245 250 255
Gly Gly Leu Ser Glu Ala Gln Ala Ile Ala Thr Thr Thr Arg Arg Thr
260 265 270
Gln Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe Arg Arg Gln His Ser
275 280 285
Pro His Trp Leu Thr Gly Gln Asp Ala Leu Ser Glu Ala Ile Arg Leu
290 295 300
Ile Glu Ala Gly Leu Gly
305 310
<210>191
<211>900
<212>DNA
<213>聚球藻属物种
<400>191
ttgaacagct ccaaccccct ggtgatcacc ctgctcgggc ccaccgccag cggcaagacc 60
gccctggcgt tggacatcgc cgaacggctg gatctgccgg tgttcaacgt ggattcccgc 120
cagctctaca gggagatgga catcggaacc gctaagccga cggcggagca acaggcgcgg 180
ataccgcacc atctgctgga cctgcgcacg cccgatcagc cgatcacgct gcaggaattt 240
caggcgatcg ccaccccctg catcaacgct gctctagagc agcgggacgt ggcgctgctg 300
gtgggcggca gcggcctcta tctcaaggcc ctcaccagcg gtcttcagcc tcctgccgtg 360
gcaccccagc cgcaactgcg ccagcagctg acggccctgg gccagcagat ctgccatccc 420
ctgctgcagg ccgccgaccc caaggccgct gccaaaatcg cccctgccga tgccgtgcgc 480
acccaacgcg cccttgaggt gctctacggc tccgggcaac cgatgagccg gcaggccaca 540
gccgcccctc cgccctggcg ggtgctggaa ctgggcctca accccgccaa cctgcgccaa 600
cgcatccagc aacgtaccga gcagctctac cgcgatgggc tggtggacga gacccagcgc 660
ctgagcgagc gctacggcgc tgatctgcca ttgctgcaga ccatcggcta cagcgaagcg 720
ctgcagatga ttggcggcag cctgaccaca acagaagcgg tgcgaatcac cagccaacgc 780
acccgccagt tcgccaaacg ccaacgcacc tggttccggc gtcagcacaa cccccactgg 840
ctgcccgacc aggcaacgct gacggatgcg atgacgttga tcgaacaaca tctaaggtga 900
<210>192
<211>299
<212>PRT
<213>聚球藻属物种
<400>192
Met Asn Ser Ser Asn Pro Leu Val Ile Thr Leu Leu Gly Pro Thr Ala
1 5 10 15
Ser Gly Lys Thr Ala Leu Ala Leu Asp Ile Ala Glu Arg Leu Asp Leu
20 25 30
Pro Val Phe Asn Val Asp Ser Arg Gln Leu Tyr Arg Glu Met Asp Ile
35 40 45
Gly Thr Ala Lys Pro Thr Ala Glu Gln Gln Ala Arg Ile Pro His His
50 55 60
Leu Leu Asp Leu Arg Thr Pro Asp Gln Pro Ile Thr Leu Gln Glu Phe
65 70 75 80
Gln Ala Ile Ala Thr Pro Cys Ile Asn Ala Ala Leu Glu Gln Arg Asp
85 90 95
Val Ala Leu Leu Val Gly Gly Ser Gly Leu Tyr Leu Lys Ala Leu Thr
100 105 110
Ser Gly Leu Gln Pro Pro Ala Val Ala Pro Gln Pro Gln Leu Arg Gln
115 120 125
Gln Leu Thr Ala Leu Gly Gln Gln Ile Cys His Pro Leu Leu Gln Ala
130 135 140
Ala Asp Pro Lys Ala Ala Ala Lys Ile Ala Pro Ala Asp Ala Val Arg
145 150 155 160
Thr Gln Arg Ala Leu Glu Val Leu Tyr Gly Ser Gly Gln Pro Met Ser
165 170 175
Arg Gln Ala Thr Ala Ala Pro Pro Pro Trp Arg Val Leu Glu Leu Gly
180 185 190
Leu Asn Pro Ala Asn Leu Arg Gln Arg Ile Gln Gln Arg Thr Glu Gln
195 200 205
Leu Tyr Arg Asp Gly Leu Val Asp Glu Thr Gln Arg Leu Ser Glu Arg
210 215 220
Tyr Gly Ala Asp Leu Pro Leu Leu Gln Thr Ile Gly Tyr Ser Glu Ala
225 230 235 240
Leu Gln Met Ile Gly Gly Ser Leu Thr Thr Thr Glu Ala Val Arg Ile
245 250 255
Thr Ser Gln Arg Thr Arg Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe
260 265 270
Arg Arg Gln His Asn Pro His Trp Leu Pro Asp Gln Ala Thr Leu Thr
275 280 285
Asp Ala Met Thr Leu Ile Glu Gln His Leu Arg
290 295
<210>193
<211>897
<212>DNA
<213>聚球藻属物种
<400>193
atgtccacac aacctctcgt gatcaccttg ctggggccca ccgccagcgg caaaaccgcc 60
ctcgcccttg agctggccga acggctcggg ctgccagtga tcaacgtgga ttcccgccag 120
ctgtatcggg agatggacgt gggcaccgcc aaacccacag ccgaacaaca ggcccgggtg 180
acgcaccact tactcgacct gcgcgatccc aaccagccga tcacgctgca ggagtttcaa 240
gcggaagcca cgccctgcat tgagcgtgaa ctaagcgaac ggggcatcgc tctacttgtg 300
gggggcagcg gtttgtacct gaaagcctta acaagcggtc tcaaaccacc agctgtgggc 360
ccgcaaccag agctgcgtaa gcaattcagt gccatgggac aagccgtctg ccatccgctc 420
ttggccgccg cggatccaat agccgctgca aaaatttccc ctgcggatgt ggtccgcacc 480
cagcgcgccc ttgaggtgct ctacgcctca ggccaaccga tgagtggtca ggccagcgtg 540
gaacctcccc cctggagaat tttggaactc ggcctaaatc ccaccaacct gcgccaacgc 600
atcaaccaac gcactgaaca gctgtatcaa gacggattag tggaggaaac ccaacgcttg 660
gccgaccgct acggagccga cttaccgctg ctgcaaacga tcggctacgg agaagcactg 720
caaatcaacg atggatcgat cacaaggcag gcggcgatcg ccaccacctg ccagcgcaca 780
cggcaattcg ccaaacgtca acgcacctgg tttcggcgcc aacacactcc ccaatggctc 840
agtgaacagg atctgttaac ggaagcgatg acgctgatcg aacaacacct aggctga 897
<210>194
<211>298
<212>PRT
<213>聚球藻属物种
<400>194
Met Ser Thr Gln Pro Leu Val Ile Thr Leu Leu Gly Pro Thr Ala Ser
1 5 10 15
Gly Lys Thr Ala Leu Ala Leu Glu Leu Ala Glu Arg Leu Gly Leu Pro
20 25 30
Val Ile Asn Val Asp Ser Arg Gln Leu Tyr Arg Glu Met Asp Val Gly
35 40 45
Thr Ala Lys Pro Thr Ala Glu Gln Gln Ala Arg Val Thr His His Leu
50 55 60
Leu Asp Leu Arg Asp Pro Asn Gln Pro Ile Thr Leu Gln Glu Phe Gln
65 70 75 80
Ala Glu Ala Thr Pro Cys Ile Glu Arg Glu Leu Ser Glu Arg Gly Ile
85 90 95
Ala Leu Leu Val Gly Gly Ser Gly Leu Tyr Leu Lys Ala Leu Thr Ser
100 105 110
Gly Leu Lys Pro Pro Ala Val Gly Pro Gln Pro Glu Leu Arg Lys Gln
115 120 125
Phe Ser Ala Met Gly Gln Ala Val Cys His Pro Leu Leu Ala Ala Ala
130 135 140
Asp Pro Ile Ala Ala Ala Lys Ile Ser Pro Ala Asp Val Val Arg Thr
145 150 155 160
Gln Arg Ala Leu Glu Val Leu Tyr Ala Ser Gly Gln Pro Met Ser Gly
165 170 175
Gln Ala Ser Val Glu Pro Pro Pro Trp Arg Ile Leu Glu Leu Gly Leu
180 185 190
Asn Pro Thr Asn Leu Arg Gln Arg Ile Asn Gln Arg Thr Glu Gln Leu
195 200 205
Tyr Gln Asp Gly Leu Val Glu Glu Thr Gln Arg Leu Ala Asp Arg Tyr
210 215 220
Gly Ala Asp Leu Pro Leu Leu Gln Thr Ile Gly Tyr Gly Glu Ala Leu
225 230 235 240
Gln Ile Asn Asp Gly Ser Ile Thr Arg Gln Ala Ala Ile Ala Thr Thr
245 250 255
Cys Gln Arg Thr Arg Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe Arg
260 265 270
Arg Gln His Thr Pro Gln Trp Leu Ser Glu Gln Asp Leu Leu Thr Glu
275 280 285
Ala Met Thr Leu Ile Glu Gln His Leu Gly
290 295
<210>195
<211>939
<212>DNA
<213>嗜热蓝细菌聚球藻(Thermosynechococcus elongatus)
<400>195
gtgggggatg cagggctaat tgttattggg ggggcaacgg ctacaggcaa aacggcgcta 60
gcgatcgccc ttgcccaaca attgaatagt gtcattttga gtgccgactc ccgccaagtc 120
taccgcggct ttgatattgg cacagccaag cccactccag cccagcaaca gcaggtgcgc 180
catcacctca tcgatatttg tgacccccgc gagacgctga ccctagcaat ttaccaagcc 240
aaggctcaag ccctgattgc ccactaccac gcccaaggga tcacgcccct cttggtgggg 300
gggacaggat tgtatatccg cagcattacc caagggctaa caatgcccca agtcccaccg 360
caaccccatc tacgggctca attgatggct ctgggtcagc aggaatgcta tcagtggctt 420
cagcaggtgg atcctgtggc ggcgcaacgg atccatgccc acgatcaagt gcgtaccctg 480
cgagccttgg aggtctatta caccactggg gtgcccctca gccagcagca acggcgcgaa 540
ccgcctccct accggatttg gtactttgcc ctcacggggg gcgatcgcca gcaagagcgc 600
tgccgtattg aagcccgcac tcaggagatg ctagccatgg gctggctcga tgaaattcag 660
caactgcaaa ggcagtacgg cgaggatttg cccctcctag acaccttggg ctatcgggaa 720
atgcgccaat atctgcgggg agaagtcacc ctcgcggagg ctattgccct cacagtgcag 780
cacacccaac aatttgccaa acggcagcgg acatggttcc gggcagaacc tgacatccat 840
tggctgcaag ccaccaccct agaggcgcaa ctggcagaga ttcaagcaca attcactctc 900
tgtcccaaga ccactgcaac aaagcggcgg atgagctga 939
<210>196
<211>312
<212>PRT
<213>嗜热蓝细菌聚球藻
<400>196
Met Gly Asp Ala Gly Leu Ile Val Ile Gly Gly Ala Thr Ala Thr Gly
1 5 10 15
Lys Thr Ala Leu Ala Ile Ala Leu Ala Gln Gln Leu Asn Ser Val Ile
20 25 30
Leu Ser Ala Asp Ser Arg Gln Val Tyr Arg Gly Phe Asp Ile Gly Thr
35 40 45
Ala Lys Pro Thr Pro Ala Gln Gln Gln Gln Val Arg His His Leu Ile
50 55 60
Asp Ile Cys Asp Pro Arg Glu Thr Leu Thr Leu Ala Ile Tyr Gln Ala
65 70 75 80
Lys Ala Gln Ala Leu Ile Ala His Tyr His Ala Gln Gly Ile Thr Pro
85 90 95
Leu Leu Val Gly Gly Thr Gly Leu Tyr Ile Arg Ser Ile Thr Gln Gly
100 105 110
Leu Thr Met Pro Gln Val Pro Pro Gln Pro His Leu Arg Ala Gln Leu
115 120 125
Met Ala Leu Gly Gln Gln Glu Cys Tyr Gln Trp Leu Gln Gln Val Asp
130 135 140
Pro Val Ala Ala Gln Arg Ile His Ala His Asp Gln Val Arg Thr Leu
145 150 155 160
Arg Ala Leu Glu Val Tyr Tyr Thr Thr Gly Val Pro Leu Ser Gln Gln
165 170 175
Gln Arg Arg Glu Pro Pro Pro Tyr Arg Ile Trp Tyr Phe Ala Leu Thr
180 185 190
Gly Gly Asp Arg Gln Gln Glu Arg Cys Arg Ile Glu Ala Arg Thr Gln
195 200 205
Glu Met Leu Ala Met Gly Trp Leu Asp Glu Ile Gln Gln Leu Gln Arg
210 215 220
Gln Tyr Gly Glu Asp Leu Pro Leu Leu Asp Thr Leu Gly Tyr Arg Glu
225 230 235 240
Met Arg Gln Tyr Leu Arg Gly Glu Val Thr Leu Ala Glu Ala Ile Ala
245 250 255
Leu Thr Val Gln His Thr Gln Gln Phe Ala Lys Arg Gln Arg Thr Trp
260 265 270
Phe Arg Ala Glu Pro Asp Ile His Trp Leu Gln Ala Thr Thr Leu Glu
275 280 285
Ala Gln Leu Ala Glu Ile Gln Ala Gln Phe Thr Leu Cys Pro Lys Thr
290 295 300
Thr Ala Thr Lys Arg Arg Met Ser
305 310
<210>197
<211>930
<212>DNA
<213>红海束毛藻(Trichodesmium erythraeum)
<400>197
ttgactttaa ttactatttg cggtccaact gctacaggta agtctggatt ggccttagat 60
ttggcccata ggttgggttc aataattctg agtgcagact cccgtcagat atataaatat 120
tttaacattg gaacggcaaa accaactata aaagaacaac agttagtatc tcattattta 180
atagatatct gtgaacctac agagattttg actttagcag aataccagaa acaggcgcaa 240
agctttattc agtctcctgg ttctcaaact gatgtggtat tgccaacatt attggtgggg 300
ggtactggtt tatacattaa ggcaatagtt aagggtttga aaattcctag ggtggggcct 360
aatgaaaagt tgcgatcgca attagcaaat ttaggtcaaa aacaatgtta caaaatgttg 420
cagcaggttg atcaggttgc cgttgagaaa attcacccga atgatgtggt ccgaacttta 480
cgagcattgg aggtatttta tgtgagtggg aaaccaattt ctcaacagca gggagaagac 540
cttccaaatt atccaatttt gcaaataggg ttagactgtg atgtagaagt tttgggggaa 600
cgtattcgtg atcggactga gaaaatggtg gaagttgggt tagtggagga agtcaagtgt 660
ttgtgtgaaa aatatggtga gaagttgcct ttattaaata ctttaggtta tgcagaaatg 720
aaacaatatt tggcgggaga tatttccttg gctgaagcga tcaaagcaac agttttacat 780
actcgtcagt ttgccaagcg acaacgcact tggtttcgag gatatccaga aattgaatgg 840
tttgatggga atgccccaaa tttattggac aaggtttggg agcgggtgag ggagtttact 900
agaatactgg cagaaaagac acaggagtga 930
<210>198
<211>309
<212>PRT
<213>红海束毛藻
<400>198
Met Thr Leu Ile Thr Ile Cys Gly Pro Thr Ala Thr Gly Lys Ser Gly
1 5 10 15
Leu Ala Leu Asp Leu Ala His Arg Leu Gly Ser Ile Ile Leu Ser Ala
20 25 30
Asp Ser Arg Gln Ile Tyr Lys Tyr Phe Asn Ile Gly Thr Ala Lys Pro
35 40 45
Thr Ile Lys Glu Gln Gln Leu Val Ser His Tyr Leu Ile Asp Ile Cys
50 55 60
Glu Pro Thr Glu Ile Leu Thr Leu Ala Glu Tyr Gln Lys Gln Ala Gln
65 70 75 80
Ser Phe Ile Gln Ser Pro Gly Ser Gln Thr Asp Val Val Leu Pro Thr
85 90 95
Leu Leu Val Gly Gly Thr Gly Leu Tyr Ile Lys Ala Ile Val Lys Gly
100 105 110
Leu Lys Ile Pro Arg Val Gly Pro Asn Glu Lys Leu Arg Ser Gln Leu
115 120 125
Ala Asn Leu Gly Gln Lys Gln Cys Tyr Lys Met Leu Gln Gln Val Asp
130 135 140
Gln Val Ala Val Glu Lys Ile His Pro Asn Asp Val Val Arg Thr Leu
145 150 155 160
Arg Ala Leu Glu Val Phe Tyr Val Ser Gly Lys Pro Ile Ser Gln Gln
165 170 175
Gln Gly Glu Asp Leu Pro Asn Tyr Pro Ile Leu Gln Ile Gly Leu Asp
180 185 190
Cys Asp Val Glu Val Leu Gly Glu Arg Ile Arg Asp Arg Thr Glu Lys
195 200 205
Met Val Glu Val Gly Leu Val Glu Glu Val Lys Cys Leu Cys Glu Lys
210 215 220
Tyr Gly Glu Lys Leu Pro Leu Leu Asn Thr Leu Gly Tyr Ala Glu Met
225 230 235 240
Lys Gln Tyr Leu Ala Gly Asp Ile Ser Leu Ala Glu Ala Ile Lys Ala
245 250 255
Thr Val Leu His Thr Arg Gln Phe Ala Lys Arg Gln Arg Thr Trp Phe
260 265 270
Arg Gly Tyr Pro Glu Ile Glu Trp Phe Asp Gly Asn Ala Pro Asn Leu
275 280 285
Leu Asp Lys Val Trp Glu Arg Val Arg Glu Phe Thr Arg Ile Leu Ala
290 295 300
Glu Lys Thr Gln Glu
305
<210>199
<211>8
<212>PRT
<213>人工序列
<220>
<223>ATP/GTP-结合基序A
<220>
<221>变体
<222>(2)..(2)
<223>/取代=″Ala″/取代=″Thr″
<220>
<221>变体
<222>(5)..(5)
<223>/取代=″Thr″/取代=″Ser″
<220>
<221>变体
<222>(8)..(8)
<223>/取代=″Thr″
<400>199
Gly Pro Thr Ala Ala Gly Lys Ser
1 5
<210>200
<211>5
<212>PRT
<213>人工序列
<220>
<223>保守基序I
<220>
<221>变体
<222>(4)..(4)
<223>/取代=″Leu″
<220>
<221>变体
<222>(5)..(5)
<223>/取代=″Leu″/取代=″Ile″
<400>200
Asp Ser Arg Gln Val
1 5
<210>201
<211>8
<212>PRT
<213>人工序列
<220>
<223>保守基序II
<220>
<221>变体
<222>(1)..(1)
<223>/取代=″Asp″/取代=″Ser″/取代=″Thr″
<220>
<221>变体
<222>(2)..(2)
<223>/取代=″Val″
<220>
<221>变体
<222>(8)..(8)
<223>/取代=″Ser″
<400>201
Asn Ile Gly Thr Ala Lys Pro Thr
1 5
<210>202
<211>8
<212>PRT
<213>人工序列
<220>
<223>保守基序III
<220>
<221>变体
<222>(2)..(2)
<223>/取代=″Ala″/取代=″Ile″
<220>
<221>变体
<222>(5)..(5)
<223>/取代=″Thr″
<400>202
Leu Val Gly Gly Ser Gly Leu Tyr
1 5
<210>203
<211>10
<212>PRT
<213>人工序列
<220>
<223>保守基序IV
<220>
<221>变体
<222>(1)..(1)
<223>/取代=″Tyr″/取代=″Leu″
<220>
<221>变体
<222>(4)..(4)
<223>/取代=″Lys″/取代=″Gln″
<220>
<221>变体
<222>(6)..(6)
<223>/取代=″Lys″/取代=″Met″
<400>203
Phe Ala Lys Arg Gln Arg Thr Trp Phe Arg
1 5 10
<210>204
<211>1553
<212>DNA
<213>稻
<400>204
gtcaccaccg tcatgtacga ggctgctcca ccactgcctc actcgccacc agcgtctccc 60
gccgcgtgca atacaagaag aaacatcgaa cggtcatata aggtaagacc cactaccgat 120
ttaacctatc cttcccacaa tctaatccac tcatttctcc tcccacgatc ttattctctc 180
atttctcctc actatttttg catttgtagg aaacacaatg acaccgtcga agaaagctgg 240
tggagcaccg tagccagcaa tcaccaaaac acagagggga ggaggtcggc agcggccatg 300
cggacggcga cgagacaacg tgacgcaaag agggaggagg acgttggcga tcatgctggt 360
gttggcggag gaggtcactg gccacgcgga tgacagcggg gcagcgcaac acaaaaaggg 420
gggaggatgc cggcgaccac gctagtgacc atgaagcaag atgatgtgaa agggaggacc 480
ggacgagggt tggacctctg ccgctgacat gaagagcgtg atgtgtagaa ggagatgtta 540
gaccagatgc cgacgcaact agccctgcaa ggtcacccga ctgatatcgc tgcttgccct 600
tgtcctcatg tacacaatca gcttgcttat ctctccatac tggtcgtttg tttcccgtgg 660
ccgaaataga agaagacaga ggtaggtttt gttagagaat tttagtggta ttgtagccta 720
tttgtaattt tgttgtactt tattgtatta atcaataaag gtgtttcatt ctattttgac 780
tcaatgttga atccattgat ctcttggtgt tgcactcagt atgttagaat attacattcc 840
gttgaaacaa tcttggttaa gggttggaac atttttatct gttcgtgaaa catccgtaat 900
attttcgttg aaacaatttt tatcgacagc accgtccaac aatttacacc aatttggacg 960
tgtgatacat agcagtcccc aagtgaaact gaccaccagt tgaaaggtat acaaagtgaa 1020
cttattcatc taaaagaccg cagagatggg ccgtggccgt ggctgcgaaa cgcagcgttc 1080
aggcccatga gcatttattt tttaaaaaaa tatttcacaa caaaaaagag aacggataaa 1140
atccatcgaa aaaaaaaact ttcctacgca tcctctccta tctccatcca cggcgagcac 1200
tcatccaaac cgtccatcca cgcgcacagt acacacacat agttatcgtc tctccccccg 1260
atgagtcacc acccgtgtct tcgagaaacg cctcgcccga caccgtacgt ggcgccaccg 1320
ccgcgcctgc cgcctggaca cgtccggctc ctctccacgc cgcgctggcc accgtccacc 1380
ggctcccgca cacgtctccc tgtctccctc cacccatgcc gtggcaatcg agctcatctc 1440
ctcgcctcct ccggcttata aatggcggcc accaccttca cctgcttgca caccacagca 1500
agagctaagt gagctagcca ctgatcagaa gaacacctcg atctctgaga gtg 1553
<210>205
<211>1300
<212>DNA
<213>稻
<400>205
ttcttctgaa gctgaagccc tgcgaaatag gcctttaaac gctttaaggt tactggatga 60
tcatatcggc gtaagaccgg tttaaacatg gtttcgcttt gtgaatccaa tgtgagtcac 120
gacgtgacac atggcacgtc cttggagctt tagacatatc gaatctgagc actggagtgg 180
ccgagtgggt gagcggccaa atccgtttta gacagatcgc actgacacga tgttgatcat 240
tgatactaat accattttat caagcagtag tgttgaaaaa gaaacttatg ttctcttcaa 300
ctgtgagatt tcatcccgtt tcaagatgaa caagccatgc atgtgagatg tgaacagaag 360
gcagaagaca gtggaaagac aggacaaata agtgaagagg gatcaaatca atgggcctga 420
cggtttctga aagttgacat ggaaatcgcc ggtgatcacc ggtttatacg ttatttaaat 480
ctgcgatttc cactttcgtt tgctttcggg gttccgattt gagtcacgca catattcttc 540
atcgtgcttt ggatctcagc accgtagtaa cttttggaca aattgcattc gccgacacta 600
ataacatgtt ctctttatgc tgctttacat atactgctta tccacaccca atcccatgtt 660
catatattat gagatggagg gagtaaactt tgttaacagc aacatttttt atattaaagc 720
atcaactaat taaagcacaa gatacgcatg ttatctcaat aaatcttcca gtgcatgtat 780
aaagaagatg tcgccgctaa cttagataat ttttgtgacc tttatcctgg ccggcataat 840
taattcttcc ggaaattaaa agctagtttt tccatattca tcagtacaga caagacagca 900
tagtaagcga agcatacctg acgtgttagc tcattgtgac tcgatctgga acactcgatg 960
ctagacacag acagacactc ctcgtgatga acgttagcat ttagcaacat acggtgataa 1020
agcagctggg gatcgatcca tccatccatc gtctttacac gtacttacct tgctaaccgc 1080
actgtcgact cttgcatgtt tgcatgtaat ccaaatggac cccacgtgga acatgctcac 1140
agtgctttgc agctgctttc caaaatgctt tctttcactt cttccattcc tctgtccaca 1200
aaaaaagtag tgtgttcttg agcctatata agagagggtc acacgctcca gtcgactcac 1260
catcgatcca tctgacggtt agttccaagg gaaagaagaa 1300
<210>206
<211>55
<212>DNA
<213>人工序列
<220>
<223>引物:prm07646
<400>206
ggggacaagt ttgtacaaaa aagcaggctt aaacaatgga atcgcgtttg aaacc 55
<210>207
<211>50
<212>DNA
<213>人工序列
<220>
<223>引物:prm07645
<400>207
ggggaccact ttgtacaaga aagctgggtt caaacgccct cactctttcg 50
<210>208
<211>1727
<212>DNA
<213>拟南芥
<400>208
caatgctttt ttataatgcc aactttgtac aaaaaagcag gcttaaacaa tggatactct 60
ctttagacta gtctgtctcc aacaacaaca acaatccgat agtatcatta caaatcaatc 120
ttcgttaagc agaacttcca ccaccactac tggctctcca caaactgctt gtcactacaa 180
ctttccacaa aacgacgtcg tcgaagaatg cttcaacttt ttcatggatg aagaagacct 240
ttcctcttct tcttctcacc acaaccatca caaccacaac aatcctaata cttactactc 300
tcctttcact actcccaccc aataccatcc cgccacatca tcaacccctt cctccaccgc 360
cgcagccgca gctttagcct cgccttactc ctcctccggc caccataatg acccttccgc 420
gttctccata cctcaaactc ctccgtcctt cgacttctca gccaatgcca agtgggcaga 480
ctcggtcctt cttgaagcgg cacgtgcctt ctccgacaaa gacactgcac gtgcgcaaca 540
aatcctatgg acgctcaacg agctctcttc tccgtacgga gacaccgagc aaaaactggc 600
ttcttacttc ctccaagctc tcttcaaccg catgaccggt tcaggcgaac gatgctaccg 660
aaccatggta acagctgcag ccacagagaa gacttgctcc ttcgagtcaa cgcgaaaaac 720
tgtactaaag ttccaagaag ttagcccctg ggccacgttt ggacacgtgg cggcaaacgg 780
agcaatcttg gaagcagtag acggagaggc aaagatccac atcgttgaca taagctccac 840
gttttgcact caatggccga ctcttctaga agctttagcc acaagatcag acgacacgcc 900
tcacctaagg ctaaccacag ttgtcgtggc caacaagttt gtcaacgatc aaacggcgtc 960
gcatcggatg atgaaagaga tcggaaaccg aatggagaaa ttcgctaggc ttatgggagt 1020
tcctttcaaa tttaacatta ttcatcacgt tggagattta tctgagtttg atctcaacga 1080
actcgacgtt aaaccagacg aagtcttggc cattaactgc gtaggcgcga tgcatgggat 1140
cgcttcacgt ggaagcccta gagacgctgt gatatcgagt ttccgacggt taagaccgag 1200
gattgtgacg gtcgtagaag aagaagctga tcttgtcgga gaagaagaag gtggctttga 1260
tgatgagttc ttgagagggt ttggagaatg tttacgatgg tttagggttt gcttcgagtc 1320
atgggaagag agttttccaa ggacgagcaa cgagaggttg atactagagc gtgcagcggg 1380
acgtgcgatc gttgatcttg tggcttgtga gccgtcggat tccacggaga ggcgagagac 1440
agcgaggaag tggtcgagga ggatgaggaa tagtgggttt ggagcggtgg ggtatagtga 1500
tgaggtggcg gatgatgtca gagctttgtt gaggagatat aaagaaggtg tttggtcgat 1560
ggtacagtgt cctgatgccg ccggaatatt cctttgttgg agagatcagc cggtggtttg 1620
ggctagtgcg tggcggccaa cgtaaagggt tgtttttatt tacccagctt tcttgtacaa 1680
agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaac 1727
<210>209
<211>531
<212>PRT
<213>拟南芥
<400>209
Met Asp Thr Leu Phe Arg Leu Val Cys Leu Gln Gln Gln Gln Gln Ser
1 5 10 15
Asp Ser Ile Ile Thr Asn Gln Ser Ser Leu Ser Arg Thr Ser Thr Thr
20 25 30
Thr Thr Gly Ser Pro Gln Thr Ala Cys His Tyr Asn Phe Pro Gln Asn
35 40 45
Asp Val Val Glu Glu Cys Phe Asn Phe Phe Met Asp Glu Glu Asp Leu
50 55 60
Ser Ser Ser Ser Ser His His Asn His His Asn His Asn Asn Pro Asn
65 70 75 80
Thr Tyr Tyr Ser Pro Phe Thr Thr Pro Thr Gln Tyr His Pro Ala Thr
85 90 95
Ser Ser Thr Pro Ser Ser Thr Ala Ala Ala Ala Ala Leu Ala Ser Pro
100 105 110
Tyr Ser Ser Ser Gly His His Asn Asp Pro Ser Ala Phe Ser Ile Pro
115 120 125
Gln Thr Pro Pro Ser Phe Asp Phe Ser Ala Asn Ala Lys Trp Ala Asp
130 135 140
Ser Val Leu Leu Glu Ala Ala Arg Ala Phe Ser Asp Lys Asp Thr Ala
145 150 155 160
Arg Ala Gln Gln Ile Leu Trp Thr Leu Asn Glu Leu Ser Ser Pro Tyr
165 170 175
Gly Asp Thr Glu Gln Lys Leu Ala Ser Tyr Phe Leu Gln Ala Leu Phe
180 185 190
Asn Arg Met Thr Gly Ser Gly Glu Arg Cys Tyr Arg Thr Met Val Thr
195 200 205
Ala Ala Ala Thr Glu Lys Thr Cys Ser Phe Glu Ser Thr Arg Lys Thr
210 215 220
Val Leu Lys Phe Gln Glu Val Ser Pro Trp Ala Thr Phe Gly His Val
225 230 235 240
Ala Ala Asn Gly Ala Ile Leu Glu Ala Val Asp Gly Glu Ala Lys Ile
245 250 255
His Ile Val Asp Ile Ser Ser Thr Phe Cys Thr Gln Trp Pro Thr Leu
260 265 270
Leu Glu Ala Leu Ala Thr Arg Ser Asp Asp Thr Pro His Leu Arg Leu
275 280 285
Thr Thr Val Val Val Ala Asn Lys Phe Val Asn Asp Gln Thr Ala Ser
290 295 300
His Arg Met Met Lys Glu Ile Gly Asn Arg Met Glu Lys Phe Ala Arg
305 310 315 320
Leu Met Gly Val Pro Phe Lys Phe Asn Ile Ile His His Val Gly Asp
325 330 335
Leu Ser Glu Phe Asp Leu Asn Glu Leu Asp Val Lys Pro Asp Glu Val
340 345 350
Leu Ala Ile Asn Cys Val Gly Ala Met His Gly Ile Ala Ser Arg Gly
355 360 365
Ser Pro Arg Asp Ala Val Ile Ser Ser Phe Arg Arg Leu Arg Pro Arg
370 375 380
Ile Val Thr Val Val Glu Glu Glu Ala Asp Leu Val Gly Glu Glu Glu
385 390 395 400
Gly Gly Phe Asp Asp Glu Phe Leu Arg Gly Phe Gly Glu Cys Leu Arg
405 410 415
Trp Phe Arg Val Cys Phe Glu Ser Trp Glu Glu Ser Phe Pro Arg Thr
420 425 430
Ser Asn Glu Arg Leu Ile Leu Glu Arg Ala Ala Gly Arg Ala Ile Val
435 440 445
Asp Leu Val Ala Cys Glu Pro Ser Asp Ser Thr Glu Arg Arg Glu Thr
450 455 460
Ala Arg Lys Trp Ser Arg Arg Met Arg Asn Ser Gly Phe Gly Ala Val
465 470 475 480
Gly Tyr Ser Asp Glu Val Ala Asp Asp Val Arg Ala Leu Leu Arg Arg
485 490 495
Tyr Lys Glu Gly Val Trp Ser Met Val Gln Cys Pro Asp Ala Ala Gly
500 505 510
Ile Phe Leu Cys Trp Arg Asp Gln Pro Val Val Trp Ala Ser Ala Trp
515 520 525
Arg Pro Thr
530
<210>210
<211>1596
<212>DNA
<213>拟南芥
<400>210
atggatactc tctttagact agtcagtctc caacaacaac aacaatccga tagtatcatt 60
acaaatcaat cttcgttaag cagaacttcc accaccacta ctggctctcc acaaactgct 120
tatcactaca actttccaca aaacgacgtc gtcgaagaat gcttcaactt tttcatggat 180
gaagaagacc tttcctcttc ttcttctcac cacaaccatc acaaccacaa caatcctaat 240
acttactact ctcctttcac tactcccacc caataccatc ccgccacatc atcaacccct 300
tcctccaccg ccgcagccgc agctttagcc tcgccttact cctcctccgg ccaccataat 360
gacccttccg cgttctccat acctcaaact cctccgtcct tcgacttctc agccaatgcc 420
aagtgggcag actcggtcct tcttgaagcg gcacgtgcct tctccgacaa agacactgca 480
cgtgcgcaac aaatcctatg gacgctcaac gagctctctt ctccgtacgg agacaccgag 540
caaaaactgg cttcttactt cctccaagct ctcttcaacc gcatgaccgg ttcaggcgaa 600
cgatgctacc gaaccatggt aacagctgca gccacagaga agacttgctc cttcgagtca 660
acgcgaaaaa ctgtactaaa gttccaagaa gttagcccct gggccacgtt tggacacgtg 720
gcggcaaacg gagcaatctt ggaagcagta gacggagagg caaagatcca catcgttgac 780
ataagctcca cgttttgcac tcaatggccg actcttctag aagctttagc cacaagatca 840
gacgacacgc ctcacctaag gctaaccaca gttgtcgtgg ccaacaagtt tgtcaacgat 900
caaacggcgt cgcatcggat gatgaaagag atcggaaacc gaatggagaa attcgctagg 960
cttatgggag ttcctttcaa atttaacatt attcatcacg ttggagattt atctgagttt 1020
gatctcaacg aactcgacgt taaaccagac gaagtcttgg ccattaactg cgtaggcgcg 1080
atgcatggga tcgcttcacg tggaagccct agagacgctg tgatatcgag tttccgacgg 1140
ttaagaccga ggattgtgac ggtcgtagaa gaagaagctg atcttgtcgg agaagaagaa 1200
ggtggctttg atgatgagtt cttgagaggg tttggagaat gtttacgatg gtttagggtt 1260
tgcttcgagt catgggaaga gagttttcca aggacgagca acgagaggtt gatgctagag 1320
cgtgcagcgg gacgtgcgat cgttgatctt gtggcttgtg agccgtcgga ttccacggag 1380
aggcgagaga cagcgaggaa gtggtcgagg aggatgagga atagtgggtt tggagcggtg 1440
gggtatagtg atgaggtggc ggatgatgtc agagctttgt tgaggagata taaagaaggt 1500
gtttggtcga tggtacagtg tcctgatgcc gccggaatat tcctttgttg gagagatcag 1560
ccggtggttt gggctagtgc gtggcggcca acgtaa 1596
<210>211
<211>531
<212>PRT
<213>拟南芥
<400>211
Met Asp Thr Leu Phe Arg Leu Val Ser Leu Gln Gln Gln Gln Gln Ser
1 5 10 15
Asp Ser Ile Ile Thr Asn Gln Ser Ser Leu Ser Arg Thr Ser Thr Thr
20 25 30
Thr Thr Gly Ser Pro Gln Thr Ala Tyr His Tyr Asn Phe Pro Gln Asn
35 40 45
Asp Val Val Glu Glu Cys Phe Asn Phe Phe Met Asp Glu Glu Asp Leu
50 55 60
Ser Ser Ser Ser Ser His His Asn His His Asn His Asn Asn Pro Asn
65 70 75 80
Thr Tyr Tyr Ser Pro Phe Thr Thr Pro Thr Gln Tyr His Pro Ala Thr
85 90 95
Ser Ser Thr Pro Ser Ser Thr Ala Ala Ala Ala Ala Leu Ala Ser Pro
100 105 110
Tyr Ser Ser Ser Gly His His Asn Asp Pro Ser Ala Phe Ser Ile Pro
115 120 125
Gln Thr Pro Pro Ser Phe Asp Phe Ser Ala Asn Ala Lys Trp Ala Asp
130 135 140
Ser Val Leu Leu Glu Ala Ala Arg Ala Phe Ser Asp Lys Asp Thr Ala
145 150 155 160
Arg Ala Gln Gln Ile Leu Trp Thr Leu Asn Glu Leu Ser Ser Pro Tyr
165 170 175
Gly Asp Thr Glu Gln Lys Leu Ala Set Tyr Phe Leu Gln Ala Leu Phe
180 185 190
Asn Arg Met Thr Gly Ser Gly Glu Arg Cys Tyr Arg Thr Met Val Thr
195 200 205
Ala Ala Ala Thr Glu Lys Thr Cys Ser Phe Glu Ser Thr Arg Lys Thr
210 215 220
Val Leu Lys Phe Gln Glu Val Ser Pro Trp Ala Thr Phe Gly His Val
225 230 235 240
Ala Ala Asn Gly Ala Ile Leu Glu Ala Val Asp Gly Glu Ala Lys Ile
245 250 255
His Ile Val Asp Ile Ser Ser Thr Phe Cys Thr Gln Trp Pro Thr Leu
260 265 270
Leu Glu Ala Leu Ala Thr Arg Ser Asp Asp Thr Pro His Leu Arg Leu
275 280 285
Thr Thr Val Val Val Ala Asn Lys Phe Val Asn Asp Gln Thr Ala Ser
290 295 300
His Arg Met Met Lys Glu Ile Gly Asn Arg Met Glu Lys Phe Ala Arg
305 310 315 320
Leu Met Gly Val Pro Phe Lys Phe Asn Ile Ile His His Val Gly Asp
325 330 335
Leu Ser Glu Phe Asp Leu Asn Glu Leu Asp Val Lys Pro Asp Glu Val
340 345 350
Leu Ala Ile Asn Cys Val Gly Ala Met His Gly Ile Ala Ser Arg Gly
355 360 365
Ser Pro Arg Asp Ala Val Ile Ser Ser Phe Arg Arg Leu Arg Pro Arg
370 375 380
Ile Val Thr Val Val Glu Glu Glu Ala Asp Leu Val Gly Glu Glu Glu
385 390 395 400
Gly Gly Phe Asp Asp Glu Phe Leu Arg Gly Phe Gly Glu Cys Leu Arg
405 410 415
Trp Phe Arg Val Cys Phe Glu Ser Trp Glu Glu Ser Phe Pro Arg Thr
420 425 430
Ser Asn Glu Arg Leu Met Leu Glu Arg Ala Ala Gly Arg Ala Ile Val
435 440 445
Asp Leu Val Ala Cys Glu Pro Ser Asp Ser Thr Glu Arg Arg Glu Thr
450 455 460
Ala Arg Lys Trp Ser Arg Arg Met Arg Asn Ser Gly Phe Gly Ala Val
465 470 475 480
Gly Tyr Ser Asp Glu Val Ala Asp Asp Val Arg Ala Leu Leu Arg Arg
485 490 495
Tyr Lys Glu Gly Val Trp Ser Met Val Gln Cys Pro Asp Ala Ala Gly
500 505 510
Ile Phe Leu Cys Trp Arg Asp Gln Pro Val Val Trp Ala Ser Ala Trp
515 520 525
Arg Pro Thr
530
<210>212
<211>1509
<212>DNA
<213>火炬松(Pinus taeda)
<400>212
atggatagat tgttcacctc cagattagca gattatcaat ccgaacaccc tctgtcatgc 60
ttcaattcta acaaaaattg tgaagatgca gacaccccta gacacataga cagctacaac 120
aaccatctca gccagtttgt tctcccatcc agaagggata aatcaaggca atgcaattct 180
ttcatggagg acgaagactt ctcattcaag caattccttc ctttcaaaga aatgttcaac 240
agcaatcaga ccggaaaggg taccacggga cctaaccaat ttagcggcac tagggctact 300
tctagcagat caagtgaagt accagacccc agcttgtttt ctgaactgaa ccctaatttt 360
ccagaggagt ttggaccagc aggatcacgt cgatgggctt caaatcttct gctagaatgt 420
gccagagcaa tagcagagaa tgaaaaaagc cgaacccaac atctgctatg gatgctaaat 480
gaactatcat ctccctatgg ggattgtgag cagaaattgg cctcgtactt cttgcaggcc 540
tttttttgca aaataacaga tactggtcct cgttgctaca ccacactttg ttcagctgct 600
gaaaaaacat actcatttga ttccacaaga aaaatgatct tgaaattcca ggaatcaagc 660
ccatggacaa cctttggtca tgttgctgcc aatggagcaa tcctggagtc ctttgaggga 720
gaaatgaagc tgcacatagt tgacctgagc aacacttttt gcacacagtg gcctactttg 780
ttagaggcct tggccaccag aagtgatgac acccctcatc ttaggttgac tactgtagtc 840
accagcaagg aagccacagc aatgaaggtc atgaaggaaa tagggcaaag aatggagaaa 900
tttgcacggc tcatgggggt tccatttgaa ttcagtgtta ttcatcaaca acatctccac 960
aagctcaacg ttggtgccct taaaatcaga ccagatgaag ctctggccat caactgcatc 1020
cacagcctgc aacgtgttat caagaatgga agggattcca tattgtccac gttctacagc 1080
atgaacccca agatagttac tgttgtagag gacgaagtag acctgactca tgaagatttt 1140
ggtgattgtt ttagtgaatg ccttcgtttt ttttccttgt tttttgattc tctagaggag 1200
agcttctcca gaaccagcaa cgagagatta atgcttgaaa gaaccagtgc aaggagcatt 1260
gtgaacatat tggcctgtga ggattctgaa gtttatgagc gcagggaaaa gggtgcacag 1320
tgggcttgga ggctcaagga ggcaggattt atacatgctg cattcagtga tgatgttgtt 1380
gatgatgtta gggctcttct caagagatac aaggagggtt ggggtcactg tagcaattca 1440
gatgggcttt tcttgacctg gaaggagcag tgcgccattt gggcttctgc ctggaagcca 1500
tgcttgtaa 1509
<210>213
<211>502
<212>PRT
<213>火炬松
<400>213
Met Asp Arg Leu Phe Thr Ser Arg Leu Ala Asp Tyr Gln Ser Glu His
1 5 10 15
Pro Leu Ser Cys Phe Asn Ser Asn Lys Asn Cys Glu Asp Ala Asp Thr
20 25 30
Pro Arg His Ile Asp Ser Tyr Asn Asn His Leu Ser Gln Phe Val Leu
35 40 45
Pro Ser Arg Arg Asp Lys Ser Arg Gln Cys Asn Ser Phe Met Glu Asp
50 55 60
Glu Asp Phe Ser Phe Lys Gln Phe Leu Pro Phe Lys Glu Met Phe Asn
65 70 75 80
Ser Asn Gln Thr Gly Lys Gly Thr Thr Gly Pro Asn Gln Phe Ser Gly
85 90 95
Thr Arg Ala Thr Ser Ser Arg Ser Ser Glu Val Pro Asp Pro Ser Leu
100 105 110
Phe Ser Glu Leu Asn Pro Asn Phe Pro Glu Glu Phe Gly Pro Ala Gly
115 120 125
Ser Arg Arg Trp Ala Ser Asn Leu Leu Leu Glu Cys Ala Arg Ala Ile
130 135 140
Ala Glu Asn Glu Lys Ser Arg Thr Gln His Leu Leu Trp Met Leu Asn
145 150 155 160
Glu Leu Ser Ser Pro Tyr Gly Asp Cys Glu Gln Lys Leu Ala Ser Tyr
165 170 175
Phe Leu Gln Ala Phe Phe Cys Lys Ile Thr Asp Thr Gly Pro Arg Cys
180 185 190
Tyr Thr Thr Leu Cys Ser Ala Ala Glu Lys Thr Tyr Ser Phe Asp Ser
195 200 205
Thr Arg Lys Met Ile Leu Lys Phe Gln Glu Ser Ser Pro Trp Thr Thr
210 215 220
Phe Gly His Val Ala Ala Asn Gly Ala Ile Leu Glu Ser Phe Glu Gly
225 230 235 240
Glu Met Lys Leu His Ile Val Asp Leu Ser Asn Thr Phe Cys Thr Gln
245 250 255
Trp Pro Thr Leu Leu Glu Ala Leu Ala Thr Arg Ser Asp Asp Thr Pro
260 265 270
His Leu Arg Leu Thr Thr Val Val Thr Ser Lys Glu Ala Thr Ala Met
275 280 285
Lys Val Met Lys Glu Ile Gly Gln Arg Met Glu Lys Phe Ala Arg Leu
290 295 300
Met Gly Val Pro Phe Glu Phe Ser Val Ile His Gln Gln His Leu His
305 310 315 320
Lys Leu Asn Val Gly Ala Leu Lys Ile Arg Pro Asp Glu Ala Leu Ala
325 330 335
Ile Asn Cys Ile His Ser Leu Gln Arg Val Ile Lys Asn Gly Arg Asp
340 345 350
Ser Ile Leu Ser Thr Phe Tyr Ser Met Asn Pro Lys Ile Val Thr Val
355 360 365
Val Glu Asp Glu Val Asp Leu Thr His Glu Asp Phe Gly Asp Cys Phe
370 375 380
Ser Glu Cys Leu Arg Phe Phe Ser Leu Phe Phe Asp Ser Leu Glu Glu
385 390 395 400
Ser Phe Ser Arg Thr Ser Asn Glu Arg Leu Met Leu Glu Arg Thr Ser
405 410 415
Ala Arg Ser Ile Val Asn Ile Leu Ala Cys Glu Asp Ser Glu Val Tyr
420 425 430
Glu Arg Arg Glu Lys Gly Ala Gln Trp Ala Trp Arg Leu Lys Glu Ala
435 440 445
Gly Phe Ile His Ala Ala Phe Ser Asp Asp Val Val Asp Asp Val Arg
450 455 460
Ala Leu Leu Lys Arg Tyr Lys Glu Gly Trp Gly His Cys Ser Asn Ser
465 470 475 480
Asp Gly Leu Phe Leu Thr Trp Lys Glu Gln Cys Ala Ile Trp Ala Ser
485 490 495
Ala Trp Lys Pro Cys Leu
500
<210>214
<211>1131
<212>DNA
<213>展叶剑叶藓(Physcomitrella patens)
<400>214
tgggccaaag ctcttcttct ggactgtgca agagctatag ctgagaaaga cacttcgcgc 60
gttcaaagca ttatgtggat tttgaacgag agtgcatctc catacggaga ttccgaccaa 120
agacttatgt catatttcgt ccaagcactt gtctgcaaga tcacagacac gggctctcga 180
tgccaccgaa gtctaacttc cgccgccgaa aagacgtatt cctttgaatc gatgcgaaat 240
atgatcctta actttcagca gaatgcgagc ccctggacaa cttttgggca tgttgctgca 300
aacggagctc ttctggagac gatggaaggg gagtccaaga tacatatcat tgacatcagc 360
agtacattgt gtacgcaatg gccaaccttt ctggaagctc tagcaacgcg gacggatata 420
acccctcacc tccgcctcac atgcatcgtg atatcacccg aagaagcagc gttgagggtg 480
atgaaacaag tcatgaacag aatagacagg tttgccagac tgatgggagt gccgttcgag 540
agcacggtga tccataaacc tcacttggaa acgttggatc tggatgagct gaacttgcgg 600
gaaggtgaag ctctcgctgt gaattgcgtt cagactctac accacatctc cgagtgtgta 660
gcagccgagg agcagtacag tcctcgagac cgaattctgt ccactttccg gagtgccaaa 720
cccaagattc tgtcgatcgt ggaggatgaa gctaacatga tctcaccaga cttccttggt 780
tgcttccggg aggcccttag attttactcg ctcttatttg aatctttgga ggagagcttt 840
ccccgagcca gcaacgagcg tctcatgctt gagcgaaatt gcgcccgcaa gctggtgaat 900
atgctaagtt ttgacgccac ggagagtaca gaaagacaag agaaagggat tcattgggat 960
taccgactac gaaaagtggg ctttgagccc gtgtcattca gtcacgacgt ggtggatgac 1020
gttcaagctc tactcaagag atacaagaag gggtggggtc ttgacatcac cgatgccagg 1080
ctttacctta cctggaagga acaagcagtt atttgcagca ccacatggaa g 1131
<210>215
<211>377
<212>PRT
<213>展叶剑叶藓
<400>215
Trp Ala Lys Ala Leu Leu Leu Asp Cys Ala Arg Ala Ile Ala Glu Lys
1 5 10 15
Asp Thr Ser Arg Val Gln Ser Ile Met Trp Ile Leu Asn Glu Ser Ala
20 25 30
Ser Pro Tyr Gly Asp Ser Asp Gln Arg Leu Met Ser Tyr Phe Val Gln
35 40 45
Ala Leu Val Cys Lys Ile Thr Asp Thr Gly Ser Arg Cys His Arg Ser
50 55 60
Leu Thr Ser Ala Ala Glu Lys Thr Tyr Ser Phe Glu Ser Met Arg Asn
65 70 75 80
Met Ile Leu Asn Phe Gln Gln Asn Ala Ser Pro Trp Thr Thr Phe Gly
85 90 95
His Val Ala Ala Asn Gly Ala Leu Leu Glu Thr Met Glu Gly Glu Ser
100 105 110
Lys Ile His Ile Ile Asp Ile Ser Ser Thr Leu Cys Thr Gln Trp Pro
115 120 125
Thr Phe Leu Glu Ala Leu Ala Thr Arg Thr Asp Ile Thr Pro His Leu
130 135 140
Arg Leu Thr Cys Ile Val Ile Ser Pro Glu Glu Ala Ala Leu Arg Val
145 150 155 160
Met Lys Gln Val Met Asn Arg Ile Asp Arg Phe Ala Arg Leu Met Gly
165 170 175
Val Pro Phe Glu Ser Thr Val Ile His Lys Pro His Leu Glu Thr Leu
180 185 190
Asp Leu Asp Glu Leu Asn Leu Arg Glu Gly Glu Ala Leu Ala Val Asn
195 200 205
Cys Val Gln Thr Leu His His Ile Ser Glu Cys Val Ala Ala Glu Glu
210 215 220
Gln Tyr Ser Pro Arg Asp Arg Ile Leu Ser Thr Phe Arg Ser Ala Lys
225 230 235 240
Pro Lys Ile Leu Ser Ile Val Glu Asp Glu Ala Asn Met Ile Ser Pro
245 250 255
Asp Phe Leu Gly Cys Phe Arg Glu Ala Leu Arg Phe Tyr Ser Leu Leu
260 265 270
Phe Glu Ser Leu Glu Glu Ser Phe Pro Arg Ala Ser Asn Glu Arg Leu
275 280 285
Met Leu Glu Arg Asn Cys Ala Arg Lys Leu Val Asn Met Leu Ser Phe
290 295 300
Asp Ala Thr Glu Ser Thr Glu Arg Gln Glu Lys Gly Ile His Trp Asp
305 310 315 320
Tyr Arg Leu Arg Lys Val Gly Phe Glu Pro Val Ser Phe Ser His Asp
325 330 335
Val Val Asp Asp Val Gln Ala Leu Leu Lys Arg Tyr Lys Lys Gly Trp
340 345 350
Gly Leu Asp Ile Thr Asp Ala Arg Leu Tyr Leu Thr Trp Lys Glu Gln
355 360 365
Ala Val Ile Cys Ser Thr Thr Trp Lys
370 375
<210>216
<211>1116
<212>DNA
<213>展叶剑叶藓
<400>216
ctactggtag aatgtgcgag ggcaatcact gcaaatgatt ccgctcgtgt gaagaacttg 60
atgtgggtgt tgaatgagct gggctctccg tacggagacg ctgaccaaag agtagctgca 120
tatttcctgc aggctttgtt ttgtaagatc accaatacgg gatctagctg ctaccgcgca 180
ctcactgctg ctgcggagag gacctattcg ttcgatacat tacgaaaaat gattctcgac 240
taccaggagg caagtccgtg gacaactttc ggtcatacag ctggaaacgg agctatgatg 300
gaggcctttg aaggtgagac aaaaattcac attgtggaca tgagcagcac ctattgcacc 360
caatggccga tccttttcga agctctagca acacgagctg agggtacacc tcacctacgc 420
ctgagcacca tcgtcatatc ccccgaggag tctgccttac aggtgatgaa gcagattatg 480
accaggttgg aaagatttgc tagacttatg ggtgttccat ttgagtacgt tgtaaagcat 540
gaaccacaac tggagaaatt ggaacttgca gcgctggacc tgcggcagga cgaagtcctc 600
gccatcacgt gcaatcacac tcttcatcac gtgtcggaga ttgtgccacg aggagagcag 660
tacagtccgc gcgatgtgct cctgtgcacc ttccgaaacg caaatccgaa gattatgatc 720
cttgtggagg aagaagtgga tctgacttcg ccagatttta ttgtttgttt ctgtgaggcg 780
ctcaagtttt attccctctt gtttgaatcc ctggaggaga acttccccag aactagcaac 840
gaacgtttaa tcctggagcg gatttgtgca cggaacctgg tcaatctgat aggttgtgac 900
cctccagaga acgttgagag gcaggagacg ggaatccagt gggatctccg tttgaaaaga 960
ataggcttcg tgccctgccc cttcagcgac gatgttgttg acgacgttcg tgcgctcctc 1020
aagaggtaca aagagggatg gagcctgagc atgaacgaga acagacttta cctagcgtgg 1080
aaagagcagg tggttctatg cgccactgct tggaag 1116
<210>217
<211>372
<212>PRT
<213>展叶剑叶藓
<400>217
Leu Leu Val Glu Cys Ala Arg Ala Ile Thr Ala Asn Asp Ser Ala Arg
1 5 10 15
Val Lys Asn Leu Met Trp Val Leu Asn Glu Leu Gly Ser Pro Tyr Gly
20 25 30
Asp Ala Asp Gln Arg Val Ala Ala Tyr Phe Leu Gln Ala Leu Phe Cys
35 40 45
Lys Ile Thr Asn Thr Gly Ser Ser Cys Tyr Arg Ala Leu Thr Ala Ala
50 55 60
Ala Glu Arg Thr Tyr Ser Phe Asp Thr Leu Arg Lys Met Ile Leu Asp
65 70 75 80
Tyr Gln Glu Ala Ser Pro Trp Thr Thr Phe Gly His Thr Ala Gly Asn
85 90 95
Gly Ala Met Met Glu Ala Phe Glu Gly Glu Thr Lys Ile His Ile Val
100 105 110
Asp Met Ser Ser Thr Tyr Cys Thr Gln Trp Pro Ile Leu Phe Glu Ala
115 120 125
Leu Ala Thr Arg Ala Glu Gly Thr Pro His Leu Arg Leu Ser Thr Ile
130 135 140
Val Ile Ser Pro Glu Glu Ser Ala Leu Gln Val Met Lys Gln Ile Met
145 150 155 160
Thr Arg Leu Glu Arg Phe Ala Arg Leu Met Gly Val Pro Phe Glu Tyr
165 170 175
Val Val Lys His Glu Pro Gln Leu Glu Lys Leu Glu Leu Ala Ala Leu
180 185 190
Asp Leu Arg Gln Asp Glu Val Leu Ala Ile Thr Cys Asn His Thr Leu
195 200 205
His His Val Ser Glu Ile Val Pro Arg Gly Glu Gln Tyr Ser Pro Arg
210 215 220
Asp Val Leu Leu Cys Thr Phe Arg Asn Ala Asn Pro Lys Ile Met Ile
225 230 235 240
Leu Val Glu Glu Glu Val Asp Leu Thr Ser Pro Asp Phe Ile Val Cys
245 250 255
Phe Cys Glu Ala Leu Lys Phe Tyr Ser Leu Leu Phe Glu Ser Leu Glu
260 265 270
Glu Asn Phe Pro Arg Thr Ser Asn Glu Arg Leu Ile Leu Glu Arg Ile
275 280 285
Cys Ala Arg Asn Leu Val Asn Leu Ile Gly Cys Asp Pro Pro Glu Asn
290 295 300
Val Glu Arg Gln Glu Thr Gly Ile Gln Trp Asp Leu Arg Leu Lys Arg
305 310 315 320
Ile Gly Phe Val Pro Cys Pro Phe Ser Asp Asp Val Val Asp Asp Val
325 330 335
Arg Ala Leu Leu Lys Arg Tyr Lys Glu Gly Trp Ser Leu Ser Met Asn
340 345 350
Glu Asn Arg Leu Tyr Leu Ala Trp Lys Glu Gln Val Val Leu Cys Ala
355 360 365
Thr Ala Trp Lys
370
<210>218
<211>1812
<212>DNA
<213>稻
<400>218
atggataccc tcttcaggtt ggttagcctc caccaccatc accaccacca gcacgcggcc 60
tcaccgtcgc cgccggacca gcegcacaag tcgtacccct cctcgcgagg gagcaccagc 120
tccccctcct cccaccacac ccacaaccac acctactacc accactccca ctcccactac 180
aacaataata gcaacaccaa ctactattac cagggtggtg gaggcggcgg cggagggtac 240
tactacgcgg aggagcagca gccggcggcg tacctagaag aatgcggcaa cggccaccag 300
ttttacatgg atgaagactt ctcctcctcg tcttcctccc gccagttcca ctcgggaacg 360
ggcgcgccgt cgtcggcgcc ggtgcctcct cctccgtcgg cgacgacgtc gtccgcgggc 420
gggcacgggc tgtttgaggc ggcggacttc tcgttcccgc aggttgatat cagcctcgac 480
ttcggcggct ctccggccgt tccgtcgtcg tccggtgctg gcgccggcgc cggggcagcg 540
ccgtcgtcgt cggggaggtg ggcggcgcag ctgctgatgg agtgcgcgcg cgcggtggcg 600
gggcgcgaca gccagcgcgt gcagcagcte atgtggatgc tcaacgagct ggcctcgccg 660
tacggcgacg tcgaccagaa gctggcctcc tacttcctgc agggcctctt cgcgcggctc 720
accacctccg gcccgcgcac gctgcggacg ctcgccaccg cgtcggaccg gaacgcgtcg 780
ttcgactcca cgcgccgcac ggcgctcaag ttccaggagc tcagcccgtg gacgccgttc 840
gggcacgtcg ccgccaacgg cgccatactc gagtcgttcc tggaggccgc ggcggcgggc 900
gccgccgcct cctcctcctc gtcgtcttca tcgtcgacgc cgccgacgcg gctgcacatc 960
ctcgacctga gcaacacgtt ctgcacgcag tggccgaccc tcctggaggc gctggccacc 1020
cggtcctcgg acgacacgcc gcacctgtcc atcaccaccg tcgtgcccac ggcggcgccg 1080
tcggcggccg cgcagcgcgt gatgcgggag atcgggcagc gcctcgagaa gttcgcgcgg 1140
ctgatgggcg tcccgttcag cttccgcgcc gtgcaccact cgggggacct ggccgacctc 1200
gacctcgccg cgctggacct ccgcgagggc ggcgccaccg ccgcgctcgc cgtcaactgc 1260
gtaaacgcgc tgcgcggggt cgcgcggggg cgcgacgcgt tcgtggcgtc gctccggcgc 1320
ctggagccgc gcgtggtcac cgtcgtggag gaggaggccg acctggcggc gccggaggcg 1380
gacgcgtcgt cggaggccga caccgacgcc gcgttcgtca aggtgttcgg cgagggcctc 1440
cgcttcttct cggcgtacat ggactcgctg gaggagagct tccccaagac aagcaacgag 1500
aggctgtcac tggagagggc ggtcggccgt gccatcgtcg acctcgtgtc atgcccggcc 1560
tcccagtccg ccgagcgccg ggagaccgcc gcgtcgtggg cgcggcgcat gcggtcggcg 1620
gggttctcgc cggcggcatt cagcgaggac gtcgccgacg acgtgcggtc gcttctccgg 1680
cggtacaagg agggctggtc gatgcgggac gccggcggtg ccacggacga cgccgccggc 1740
gccgctgctg ccggagcgtt ccttgcgtgg aaggagcagc ctgtcgtgtg ggcgagcgcg 1800
tggaagccat ga 1812
<210>219
<211>603
<212>PRT
<213>稻
<400>219
Met Asp Thr Leu Phe Arg Leu Val Ser Leu His His His His His His
1 5 10 15
Gln His Ala Ala Ser Pro Ser Pro Pro Asp Gln Pro His Lys Ser Tyr
20 25 30
Pro Ser Ser Arg Gly Ser Thr Ser Ser Pro Ser Ser His His Thr His
35 40 45
Asn His Thr Tyr Tyr His His Ser His Ser His Tyr Asn Asn Asn Ser
50 55 60
Asn Thr Asn Tyr Tyr Tyr Gln Gly Gly Gly Gly Gly Gly Gly Gly Tyr
65 70 75 80
Tyr Tyr Ala Glu Glu Gln Gln Pro Ala Ala Tyr Leu Glu Glu Cys Gly
85 90 95
Asn Gly His Gln Phe Tyr Met Asp Glu Asp Phe Ser Ser Ser Ser Ser
100 105 110
Ser Arg Gln Phe His Ser Gly Thr Gly Ala Pro Ser Ser Ala Pro Val
115 120 125
Pro Pro Pro Pro Ser Ala Thr Thr Ser Ser Ala Gly Gly His Gly Leu
130 135 140
Phe Glu Ala Ala Asp Phe Ser Phe Pro Gln Val Asp Ile Ser Leu Asp
145 150 155 160
Phe Gly Gly Ser Pro Ala Val Pro Ser Ser Ser Gly Ala Gly Ala Gly
165 170 175
Ala Gly Ala Ala Pro Ser Ser Ser Gly Arg Trp Ala Ala Gln Leu Leu
180 185 190
Met Glu Cys Ala Arg Ala Val Ala Gly Arg Asp Ser Gln Arg Val Gln
195 200 205
Gln Leu Met Trp Met Leu Asn Glu Leu Ala Ser Pro Tyr Gly Asp Val
210 215 220
Asp Gln Lys Leu Ala Ser Tyr Phe Leu Gln Gly Leu Phe Ala Arg Leu
225 230 235 240
Thr Thr Ser Gly Pro Arg Thr Leu Arg Thr Leu Ala Thr Ala Ser Asp
245 250 255
Arg Asn Ala Ser Phe Asp Ser Thr Arg Arg Thr Ala Leu Lys Phe Gln
260 265 270
Glu Leu Ser Pro Trp Thr Pro Phe Gly His Val Ala Ala Asn Gly Ala
275 280 285
Ile Leu Glu Ser Phe Leu Glu Ala Ala Ala Ala Gly Ala Ala Ala Ser
290 295 300
Ser Ser Ser Ser Ser Ser Ser Ser Thr Pro Pro Thr Arg Leu His Ile
305 310 315 320
Leu Asp Leu Ser Asn Thr Phe Cys Thr Gln Trp Pro Thr Leu Leu Glu
325 330 335
Ala Leu Ala Thr Arg Ser Ser Asp Asp Thr Pro His Leu Ser Ile Thr
340 345 350
Thr Val Val Pro Thr Ala Ala Pro Ser Ala Ala Ala Gln Arg Val Met
355 360 365
Arg Glu Ile Gly Gln Arg Leu Glu Lys Phe Ala Arg Leu Met Gly Val
370 375 380
Pro Phe Ser Phe Arg Ala Val His His Ser Gly Asp Leu Ala Asp Leu
385 390 395 400
Asp Leu Ala Ala Leu Asp Leu Arg Glu Gly Gly Ala Thr Ala Ala Leu
405 410 415
Ala Val Asn Cys Val Asn Ala Leu Arg Gly Val Ala Arg Gly Arg Asp
420 425 430
Ala Phe Val Ala Ser Leu Arg Arg Leu Glu Pro Arg Val Val Thr Val
435 440 445
Val Glu Glu Glu Ala Asp Leu Ala Ala Pro Glu Ala Asp Ala Ser Ser
450 455 460
Glu Ala Asp Thr Asp Ala Ala Phe Val Lys Val Phe Gly Glu Gly Leu
465 470 475 480
Arg Phe Phe Ser Ala Tyr Met Asp Ser Leu Glu Glu Ser Phe Pro Lys
485 490 495
Thr Ser Asn Glu Arg Leu Ser LeuGlu Arg Ala Val Gly Arg Ala Ile
500 505 510
Val Asp Leu Val Ser Cys Pro Ala Ser Gln Ser Ala Glu Arg Arg Glu
515 520 525
Thr Ala Ala Ser Trp Ala Arg Arg Met Arg Ser Ala Gly Phe Ser Pro
530 535 540
Ala Ala Phe Ser Glu Asp Val Ala Asp Asp Val Arg Ser Leu Leu Arg
545 550 555 560
Arg Tyr Lys Glu Gly Trp Ser Met Arg Asp Ala Gly Gly Ala Thr Asp
565 570 575
Asp Ala Ala Gly Ala Ala Ala Ala Gly Ala Phe Leu Ala Trp Lys Glu
580 585 590
Gln Pro Val Val Trp Ala Ser Ala Trp Lys Pro
595 600
<210>220
<211>1809
<212>DNA
<213>稻
<400>220
atggatacgc tgtttaggtt ggttagcctc caagccgcct ccgagcagca gcagcagcag 60
cagcagtcgg cgtcctacaa ctcgaggagc acgacgtcga gcgggtccag gtcgtcgtcg 120
caccagacga acgcgtccta cagctactac caccacagca gcaacagcgg cggcggcggc 180
ggaggcggcg gagggtacta ctacggcggc cagcagccgc cgccgtcgca gtactactac 240
ctggagccgt accaagaaga atgcggcaac gccccacacc accagcttta catggatgaa 300
gacttctcct cctcgtcgtc gtcgaggcac ttccaccacg gcgcgcgggt gcagcagcag 360
cagccgccgg cgtcgtccac gcccacgggg acggcgccga cgccgccgct gtcgacctcg 420
tccaccgcgg cgggcgccgg gcacggcctg ttcgaggcgg cggacctgtc gttcccgccg 480
gacctcaacc tcgacttctc gtccccggcg tcgtcgtccg gcggcgggac agcgtcgtcg 540
ggcgcggttg ggggcggcgg cggcgggagg tgggctagcc agctgctgct ggagtgcgcg 600
cggtcggtgg ccgcccgcga cagccagcgc gtgcagcagc tcatgtggat gctcaacgag 660
ctcgcgtcgc cgtacggcga cgtggagcag aagctggctt cctacttctt gcaggggctg 720
ttcgctcggc tcacggcgtc cgggccgcgc acgctgcgca cgctcgccgc ggcgtccgac 780
cggaacacgt cgttcgactc gacgcggcgc acggcgctgc ggttccagga gctcagcccc 840
tggtcctcgt ttgggcacgt cgccgccaat ggcgccatcc tcgagtcctt cctggaggtc 900
gccgccgcgg cgtcgtcgga gacgcagcgg ttccacatcc tcgacctgag caacacgttc 960
tgcacgcagt ggccgacgct gctggaggcg ctggccacgc ggtccgccga cgagacgccg 1020
cacctctcga tcaccaccgt ggtgtccgcc gcgccgtccg cgcccacggc ggcggtgcag 1080
cgcgtcatgc gggagatcgg gcagcgcatg gagaagttcg cgcggctcat gggcgtgccc 1140
ttccgcttcc gcgccgtgca ccactccggg gacctcgcgg agctcgacct cgacgcgctc 1200
gacctccgcg agggcggcgc caccaccgcg ctcgccgtca actgcgtcaa ctcgctgcgc 1260
ggcgtggttc ccggcagggc ccgccggcgc gacgcgttcg cggcgtcgct ccgccggctg 1320
gacccgcggg tcgtcaccgt cgtcgaggag gaggcggacc tggtggcgtc cgatcccgac 1380
gcgtcgtcgg cgacggagga aggcggcgac acggaggcgg cgttcctcaa ggtgttcggc 1440
gagggcttgc gcttcttctc ggcgtacatg gattcgctcg aggagagctt ccccaagacg 1500
agcaacgaga ggctggcatt ggagagggga gcagggcgcg ccatcgtcga cttggtctcg 1560
tgcccggcgt cggagtcgat ggagcggcgg gagacggcgg cgtcgtgggc gcggcgcatg 1620
cggtcggccg ggttctctcc ggtggcattc agcgaggacg tcgccgacga cgtgcgatcg 1680
ctgctgcgcc ggtacaggga agggtggtcg atgcgcgagg ccggcacgga cgactcggcg 1740
gccggagccg gcgtcttcct cgcgtggaag gagcagcctc tggtgtgggc aagcgcgtgg 1800
cggccatga 1809
<210>221
<211>602
<212>PRT
<213>稻
<400>221
Met Asp Thr Leu Phe Arg Leu Val Ser Leu Gln Ala Ala Ser Glu Gln
1 5 10 15
Gln Gln Gln Gln Gln Gln Ser Ala Ser Tyr Asn Ser Arg Ser Thr Thr
20 25 30
Ser Ser Gly Ser Arg Ser Ser Ser His Gln Thr Asn Ala Ser Tyr Ser
35 40 45
Tyr Tyr His His Ser Ser Asn Ser Gly Gly Gly Gly Gly Gly Gly Gly
50 55 60
Gly Tyr Tyr Tyr Gly Gly Gln Gln Pro Pro Pro Ser Gln Tyr Tyr Tyr
65 70 75 80
Leu Glu Pro Tyr Gln Glu Glu Cys Gly Asn Ala Pro His His Gln Leu
85 90 95
Tyr Met Asp Glu Asp Phe Ser Ser Ser Ser Ser Ser Arg His Phe His
100 105 110
His Gly Ala Arg Val Gln Gln Gln Gln Pro Pro Ala Ser Ser Thr Pro
115 120 125
Thr Gly Thr Ala Pro Thr Pro Pro Leu Ser Thr Ser Ser Thr Ala Ala
130 135 140
Gly Ala Gly His Gly Leu Phe Glu Ala Ala Asp Leu Ser Phe Pro Pro
145 150 155 160
Asp Leu Asn Leu Asp Phe Ser Ser Pro Ala Ser Ser Ser Gly Gly Gly
165 170 175
Thr Ala Ser Ser Gly Ala Val Gly Gly Gly Gly Gly Gly Arg Trp Ala
180 185 190
Ser Gln Leu Leu Leu Glu Cys Ala Arg Ser Val Ala Ala Arg Asp Ser
195 200 205
Gln Arg Val Gln Gln Leu Met Trp Met Leu Asn Glu Leu Ala Ser Pro
210 215 220
Tyr Gly Asp Val Glu Gln Lys Leu Ala Ser Tyr Phe Leu Gln Gly Leu
225 230 235 240
Phe Ala Arg Leu Thr Ala Ser Gly Pro Arg Thr Leu Arg Thr Leu Ala
245 250 255
Ala Ala Ser Asp Arg Asn Thr Ser Phe Asp Ser Thr Arg Arg Thr Ala
260 265 270
Leu Arg Phe Gln Glu Leu Ser Pro Trp Ser Ser Phe Gly His Val Ala
275 280 285
Ala Asn Gly Ala Ile Leu Glu Ser Phe Leu Glu Val Ala Ala Ala Ala
290 295 300
Ser Ser Glu Thr Gln Arg Phe His Ile Leu Asp Leu Ser Asn Thr Phe
305 310 315 320
Cys Thr Gln Trp Pro Thr Leu Leu Glu Ala Leu Ala Thr Arg Ser Ala
325 330 335
Asp Glu Thr Pro His Leu Ser Ile Thr Thr Val Val Ser Ala Ala Pro
340 345 350
Ser Ala Pro Thr Ala Ala Val Gln Arg Val Met Arg Glu Ile Gly Gln
355 360 365
Arg Met Glu Lys Phe Ala Arg Leu Met Gly Val Pro Phe Arg Phe Arg
370 375 380
Ala Val His His Ser Gly Asp Leu Ala Glu Leu Asp Leu Asp Ala Leu
385 390 395 400
Asp Leu Arg Glu Gly Gly Ala Thr Thr Ala Leu Ala Val Asn Cys Val
405 410 415
Asn Ser Leu Arg Gly Val Val Pro Gly Arg Ala Arg Arg Arg Asp Ala
420 425 430
Phe Ala Ala Ser Leu Arg Arg Leu Asp Pro Arg Val Val Thr Val Val
435 440 445
Glu Glu Glu Ala Asp Leu Val Ala Ser Asp Pro Asp Ala Ser Ser Ala
450 455 460
Thr Glu Glu Gly Gly Asp Thr Glu Ala Ala Phe Leu Lys Val Phe Gly
465 470 475 480
Glu Gly Leu Arg Phe Phe Ser Ala Tyr Met Asp Ser Leu Glu Glu Ser
485 490 495
Phe Pro Lys Thr Ser Asn Glu Arg Leu Ala Leu Glu Arg Gly Ala Gly
500 505 510
Arg Ala Ile Val Asp Leu Val Ser Cys Pro Ala Ser Glu Ser Met Glu
515 520 525
Arg Arg Glu Thr Ala Ala Ser Trp Ala Arg Arg Met Arg Ser Ala Gly
530 535 540
Phe Ser Pro Val Ala Phe Ser Glu Asp Val Ala Asp Asp Val Arg Ser
545 550 555 560
Leu Leu Arg Arg Tyr Arg Glu Gly Trp Ser Met Arg Glu Ala Gly Thr
565 570 575
Asp Asp Ser Ala Ala Gly Ala 6ly Val Phe Leu Ala Trp Lys Glu Gln
580 585 590
Pro Leu Val Trp Ala Ser Ala Trp Arg Pro
595 600
<210>222
<211>1785
<212>DNA
<213>玉蜀黍
<400>222
atggatacgc tgtttaggtt ggttagcctt caagcctccg aacagcagca gcagcagcaa 60
tcggcgtcct acaactcgag gagcaccacg tcgagcggct ccaggtcgtc gtcgcaccag 120
accaacgcat cctacaacta ctactaccac agcaacagca gcggcggcgg cggcggcggg 180
cagtactact acggtcagca gcacccacag cagcaccagc accagcagta ctacctggag 240
ccgtaccagc aagaagaatg cggcaacacc caccaccttt acatggatga agacttctcc 300
tcctcgtcct cgtcgaggca gcatttccac tcgcacggcg cggtggtgca gccgccgacg 360
tcgtccacgg ccacgcccac ggcgccgacg ccctcgctgt ccacgtcgtc cacggccgct 420
ggggcggcgc acgcgctgtt cgaggcggcc gacctgtcgt tcccgcctga cctcaacctc 480
gacttctcat ccccggcctc gtcgtccggc gggggcgcgg cctcgtcggc ggcggtcggg 540
ggaggtggcg ggggaaggtg ggcgagccag ctgctgctgg agtgcgcgcg cgcggtggcg 600
ggccgcgaca gccagcgcgt gcagcagctg atgtggatgc tcaacgagct ggcctcgccg 660
tacggggacg tcgagcagaa gctggcgtcc tacttcctcc aggggctctt cgcgcgcctc 720
acggcgtccg gaccgcggac gctgcgcacg ctcgcggcgg cgtccgaccg gaacacgtcc 780
ttcgactcca cgcggcgcac ggcgctgcgg ttccaggagc tcagcccgtg gtcgtcgttt 840
gggcacgtgg ccgccaacgg cgccatcctg gagtcgttcc tggaggccgc cgcggcgtcg 900
ccggagcccc agaggctcca catcctcgac ctcagcaaca cgttctgcac gcagtggccc 960
acgctgctgg aggcgctcgc cacgcggtcc gccgacgaca cgccgcacct gtcgatcacc 1020
acggtggtct cctccgcgcc gtccgcgccg acggccgccg tgcagcgcgt gatgcgggag 1080
atcgggcagc ggatggaaaa gttcgcgcgg ctgatgggcg tgcccttcag cttccgcgca 1140
gtgcaccacg ccggggacct tgcggggctc gacctcgacg cgctcgacct gcgcgacggc 1200
ggcgccacca ccgcgctcgc catcaactgc gtcaactcgc tgcgcggcgt ggtgccgggc 1260
ggtgcgcgga gacgggacgc gttcgccgcg tccctccggc gtctcgatcc gcgggttgtt 1320
actgtcgtcg aggaggaggc cgatctcgtg gcttttgacc ccggcgcgcc cgaggaaagc 1380
ggcgacacgg aggcagcgtt cctgaaggtg ttcggcgagg gcttgcggtt cttctcggct 1440
tacatggact cgctggaaga gagcttcccc aagactagca acgagaggct ggcgctggag 1500
aggggagccg gacgtgccat tgtagacttg gtctcgtgcc cggcgtcgga gtccatggag 1560
cggcgggaga cggcggcttc atgggcgcgc cgcatgcggt cttccggctt ctctccggtg 1620
gcgttcagcg aggacgtcgc cgacgacgtg cggtcgttgc tccgtcggta tcgggaaggc 1680
tggtcgatgc gggacgccgg tttagacgac tcggcagccg gagcaggcgt cttcctggcg 1740
tggaaggaac agcctctcgt gtgggcgagc gcgtggaggc catga 1785
<210>223
<211>594
<212>PRT
<213>玉蜀黍
<400>223
Met Asp Thr Leu Phe Arg Leu Val Ser Leu Gln Ala Ser Glu Gln Gln
1 5 10 15
Gln Gln Gln Gln Ser Ala Ser Tyr Asn Ser Arg Ser Thr Thr Ser Ser
20 25 30
Gly Ser Arg Ser Ser Ser His Gln Thr Asn Ala Ser Tyr Asn Tyr Tyr
35 40 45
Tyr His Ser Asn Ser Ser Gly Gly Gly Gly Gly Gly Gln Tyr Tyr Tyr
50 55 60
Gly Gln Gln His Pro Gln Gln His Gln His Gln Gln Tyr Tyr Leu Glu
65 70 75 80
Pro Tyr Gln Gln Glu Glu Cys Gly Asn Thr His His Leu Tyr Met Asp
85 90 95
Glu Asp Phe Ser Ser Ser Ser Ser Ser Arg Gln His Phe His Ser His
100 105 110
Gly Ala Val Val Gln Pro Pro Thr Ser Ser Thr Ala Thr Pro Thr Ala
115 120 125
Pro Thr Pro Ser Leu Ser Thr Ser Ser Thr Ala Ala Gly Ala Ala His
130 135 140
Ala Leu Phe Glu Ala Ala Asp Leu Ser Phe Pro Pro Asp Leu Asn Leu
145 150 155 160
Asp Phe Ser Ser Pro Ala Ser Ser Ser Gly Gly Gly Ala Ala Ser Ser
165 170 175
Ala Ala Val Gly Gly Gly Gly Gly Gly Arg Trp Ala Ser Gln Leu Leu
180 185 190
Leu Glu Cys Ala Arg Ala Val Ala Gly Arg Asp Ser Gln Arg Val Gln
195 200 205
Gln Leu Met Trp Met Leu Asn Glu Leu Ala Ser Pro Tyr Gly Asp Val
210 215 220
Glu Gln Lys Leu Ala Ser Tyr Phe Leu Gln Gly Leu Phe Ala Arg Leu
225 230 235 240
Thr Ala Ser Gly Pro Arg Thr Leu Arg Thr Leu Ala Ala Ala Ser Asp
245 250 255
Arg Asn Thr Ser Phe Asp Ser Thr Arg Arg Thr Ala Leu Arg Phe Gln
260 265 270
Glu Leu Ser Pro Trp Ser Ser Phe Gly His Val Ala Ala Asn Gly Ala
275 280 285
Ile Leu Glu Ser Phe Leu Glu Ala Ala Ala Ala Ser Pro Glu Pro Gln
290 295 300
Arg Leu His Ile Leu Asp Leu Ser Asn Thr Phe Cys Thr Gln Trp Pro
305 310 315 320
Thr Leu Leu Glu Ala Leu Ala Thr Arg Ser Ala Asp Asp Thr Pro His
325 330 335
Leu Ser Ile Thr Thr Val Val Ser Ser Ala Pro Ser Ala Pro Thr Ala
340 345 350
Ala Val Gln Arg Val Met Arg Glu Ile Gly Gln Arg Met Glu Lys Phe
355 360 365
Ala Arg Leu Met Gly Val Pro Phe Ser Phe Arg Ala Val His His Ala
370 375 380
Gly Asp Leu Ala Gly Leu Asp Leu Asp Ala Leu Asp Leu Arg Asp Gly
385 390 395 400
Gly Ala Thr Thr Ala Leu Ala Ile Asn Cys Val Asn Ser Leu Arg Gly
405 410 415
Val Val Pro Gly Gly Ala Arg Arg Arg Asp Ala Phe Ala Ala Ser Leu
420 425 430
Arg Arg Leu Asp Pro Arg Val Val Thr Val Val Glu Glu Glu Ala Asp
435 440 445
Leu Val Ala Phe Asp Pro Gly Ala Pro Glu Glu Ser Gly Asp Thr Glu
450 455 460
Ala Ala Phe Leu Lys Val Phe Gly Glu Gly Leu Arg Phe Phe Ser Ala
465 470 475 480
Tyr Met Asp Ser Leu Glu Glu Ser Phe Pro Lys Thr Ser Asn Glu Arg
485 490 495
Leu Ala Leu Glu Arg Gly Ala Gly Arg Ala Ile Val Asp Leu Val Ser
500 505 510
Cys Pro Ala Ser Glu Ser Met Glu Arg Arg Glu Thr Ala Ala Ser Trp
515 520 525
Ala Arg Arg Met Arg Ser Ser Gly Phe Ser Pro Val Ala Phe Ser Glu
530 535 540
Asp Val Ala Asp Asp Val Arg Ser Leu Leu Arg Arg Tyr Arg Glu Gly
545 550 555 560
Trp Ser Met Arg Asp Ala Gly Leu Asp Asp Ser Ala Ala Gly Ala Gly
565 570 575
Val Phe Leu Ala Trp Lys Glu Gln Pro Leu Val Trp Ala Ser Ala Trp
580 585 590
Arg Pro
<210>224
<211>1554
<212>DNA
<213>莴苣(Lactuca sativa)
<400>224
atggatacta tgtccaccct ccgacaccaa caactcctct ctgattatca acaacaacat 60
catcatcatc atcatcgtca atctttcaac tccaccgtaa gttctagcag tcctagatcc 120
tccaccgatc atcaaacaac caccggcgcc ggccattact accaaccaca agaagaatgt 180
ttccctagta ctttcttcat ggaagaagat gatctctctt cttcttcctc attgcaacaa 240
cactactact cttctaacca gttacaaccc catttccacc accagttttc cgccactcct 300
actcctactt ccaccacaag caccaccacc actcctcctc cacctcccaa gactcatggt 360
ttttcttcta attctgatca taccaacttt aactactcgt cttcccagga tcatatcgct 420
atggagtttt cgaattcgtt ctccgccaac ggctgggcgt cggatgtgtt attggaggct 480
gcgcgtgcgg tggctgataa gaacagcacg cgtctacagc agttgatgtg gatgctcaac 540
gagctcagtt ctccctatgg cgatgtagat gagaagcttt cttgttactt tcttcaagcg 600
ctttacagtc ggatgacgga ttccggtgag ttgaattacc ggaatttatc gtcgatttcg 660
gagaaagtta gcacctttga atcaaccaga gaattggtgc tcaagtttca agaagtcagc 720
ccatggacga cgttcggaca cgttgcgtgt aacggagcga tcttagaggc gttagacggc 780
gagactaaat tgcacattgt tgatgtcagc aacacatact gcacacagtg gccgacgttg 840
ctggaagcca tcgccacccg ggcagatgaa gcaccacatc tccgcctcac gacggtgatc 900
tcctccagat cagccggcag cgacggaata gagaggatca tgcgagaaat agggagtcgg 960
atggaaaaat tcgccaggtt aatgggtgtc ccgtttaaat tcaatgtgat acaccatatc 1020
ggcgatttat ccgatctgaa ttttagtcag ttagctattc aatctgatga agctcttgct 1080
ataaacttaa atggcacgtt acgttctgtt agtaatcacc gtagggatta tttgatctcg 1140
atgtttcgga gtatgaaccc taaaataatg acgatcgtgg aagaagaagc tgatttggaa 1200
gtggggattg atgggtttga attcttgaga ggatttcaag agtgtttgaa atggtttagg 1260
gtttacttcg aagctctcaa cgaaagcttt cctcaaacga gtaccgagag attgatgttg 1320
gagagggagg cggggcgggc tgtgatggac ttggtggcat gctcgccggc gaattccgtg 1380
gagaggcggg agacggctaa ccggtggtcc agtagacttc gtgcaaatgg ttttggtggg 1440
gtttcatata gcgaagaagg gtgcgacgat gtccgggcac tgctgaggag gtataaggaa 1500
gggtggtcaa tggctccgtc ggaaacggcg gccggaattt tttttgatgc gtaa 1554
<210>225
<211>517
<212>PRT
<213>莴苣
<400>225
Met Asp Thr Met Ser Thr Leu Arg His Gln Gln Leu Leu Ser Asp Tyr
1 5 10 15
Gln Gln Gln His His His His His His Arg Gln Ser Phe Asn Ser Thr
20 25 30
Val Ser Ser Ser Ser Pro Arg Ser Ser Thr Asp His Gln Thr Thr Thr
35 40 45
Gly Ala Gly His Tyr Tyr Gln Pro Gln Glu Glu Cys Phe Pro Ser Thr
50 55 60
Phe Phe Met Glu Glu Asp Asp Leu Ser Ser Ser Ser Ser Leu Gln Gln
65 70 75 80
His Tyr Tyr Ser Ser Asn Gln Leu Gln Pro His Phe His His Gln Phe
85 90 95
Ser Ala Thr Pro Thr Pro Thr Ser Thr Thr Ser Thr Thr Thr Thr Pro
100 105 110
Pro Pro Pro Pro Lys Thr His Gly Phe Ser Ser Asn Ser Asp His Thr
115 120 125
Asn Phe Asn Tyr Ser Ser Ser Gln Asp His Ile Ala Met Glu Phe Ser
130 135 140
Asn Ser Phe Ser Ala Asn Gly Trp Ala Ser Asp Val Leu Leu Glu Ala
145 150 155 160
Ala Arg Ala Val Ala Asp Lys Asn Ser Thr Arg Leu Gln Gln Leu Met
165 170 175
Trp Met Leu Asn Glu Leu Ser Ser Pro Tyr Gly Asp Val Asp Glu Lys
180 185 190
Leu Ser Cys Tyr Phe Leu Gln Ala Leu Tyr Ser Arg Met Thr Asp Ser
195 200 205
Gly Glu Leu Asn Tyr Arg Asn Leu Ser Ser Ile Ser Glu Lys Val Ser
210 215 220
Thr Phe Glu Ser Thr Arg Glu Leu Val Leu Lys Phe Gln Glu Val Ser
22 5230 235 240
Pro Trp Thr Thr Phe Gly His Val Ala Cys Asn Gly Ala Ile Leu Glu
245 250 255
Ala Leu Asp Gly Glu Thr Lys Leu His Ile Val Asp Val Ser Asn Thr
260 265 270
Tyr Cys Thr Gln Trp Pro Thr Leu Leu Glu Ala Ile Ala Thr Arg Ala
275 280 285
Asp Glu Ala Pro His Leu Arg Leu Thr Thr Val Ile Ser Ser Arg Ser
290 295 300
Ala Gly Ser Asp Gly Ile Glu Arg Ile Met Arg Glu Ile Gly Ser Arg
305 310 315 320
Met Glu Lys Phe Ala Arg Leu Met Gly Val Pro Phe Lys Phe Asn Val
325 330 335
Ile His His Ile Gly Asp Leu Ser Asp Leu Asn Phe Ser Gln Leu Ala
340 345 350
Ile Gln Ser Asp Glu Ala Leu Ala Ile Asn Leu Asn Gly Thr Leu Arg
355 360 365
Ser Val Ser Asn His Arg Arg Asp Tyr Leu Ile Ser Met Phe Arg Ser
370 375 380
Met Asn Pro Lys Ile Met Thr Ile Val Glu Glu Glu Ala Asp Leu Glu
385 390 395 400
Val Gly Ile Asp Gly Phe Glu Phe Leu Arg Gly Phe Gln Glu Cys Leu
405 410 415
Lys Trp Phe Arg Val Tyr Phe Glu Ala Leu Asn Glu Ser Phe Pro Gln
420 425 430
Thr Ser Thr Glu Arg Leu Met Leu Glu Arg Glu Ala Gly Arg Ala Val
435 440 445
Met Asp Leu Val Ala Cys Ser Pro Ala Asn Ser Val Glu Arg Arg Glu
450 455 460
Thr Ala Asn Arg Trp Ser Ser Arg Leu Arg Ala Asn Gly Phe Gly Gly
465 470 475 480
Val Ser Tyr Ser Glu Glu Gly Cys Asp Asp Val Arg Ala Leu Leu Arg
485 490 495
Arg Tyr Lys Glu Gly Trp Ser Met Ala Pro Ser Glu Thr Ala Ala Gly
500 505 510
Ile Phe Phe Asp Ala
515
<210>226
<211>1515
<212>DNA
<213>蒺藜苜蓿
<400>226
atggatacgc tgtttaggct tgtcaatttt caacaacaac aacaacaata tcaacctgat 60
ccttccctca actccaccac caccttaaca acttctagca gctctagatc ctcaagacaa 120
acaacatatc attattacaa ccaacaagaa gaagacgaag aatgcttcaa caacttttat 180
tacatggatc ataataacaa caatgatgaa gatttatcct catcttcttc taaacaacac 240
tattatactt atccttatgc ttccactact actatcacta ctccaaacac tacttataat 300
actatcaaca cccccaccac aactgataat tactctttct caccctccca tgattacttc 360
aactttgaat tctccggtca ctcttggtca caaaacatcc tccttgaaac tgcacgtgca 420
ttttccgaca ataacacaaa ccgaatccaa caactcatgt ggatgctaaa cgagcttagt 480
accccatatg gtgacacaga tcaaaaactt tcatcttatt ttcttcaagc tttgtttagt 540
cgcatgaacg acgcgggaga tagaacctac aaaaccttaa caaccgcgtc ggaaaaaaca 600
tgttcctttg attcaacgag gaagatgttg ttgaagttcc aagaagttag tccatggaca 660
acgtttggac acgtggcagc taacggtgct atcttagaag cattggaagg taaccctaaa 720
cttcacataa ttgatattag caacacttat tgcactcaat ggccaacact cctcgaagcg 780
ttggccaccc gctctgatga tacgcctcac cttcgtttaa ccactgtagt cactgcaatc 840
agcggtggct cggtgcaaaa agtcatgaag gaaattggtt caaggatgga aaaatttgct 900
aggcttatgg gagtgccgtt taaattcaaa atcattttca gtgatttaag agagttgaat 960
ttgtgtgatt tagatattaa agaagatgaa gctttggcta taaactgtgt aaactcttta 1020
cattcgatat ccggagccgg aaaccaccgt gatttgttta tatctttgct tcgtgggtta 1080
gaacctaggg ttttaactat tgttgaagaa gaagctgatt tagaagtttg ttttggttcg 1140
gatttcgtgg aaggttttaa agagtgttta agatggttta gggtttattt tgaagcactt 1200
gatgaaagtt tttcaagaac aagtagtgag aggttgatgc tagaaagaga agctgggaga 1260
ggtattgttg atttggttgc ttgtgatcct tatgagtcag tggagagacg tgaaacggcg 1320
gcgcggtgga ggaggaggtt gcatggtggc gggtttaata cggtgtcgtt tagtgatgaa 1380
gtttgtgatg atgttagggc tttattgaga aggtataaag aagggtggtc gatgacatcc 1440
tccgatggtg ataccggaat attcttgtcg tggaaggata agccggtggt gtgggccagt 1500
gtatggaggc cttga 1515
<210>227
<211>504
<212>PRT
<213>蒺藜苜蓿
<400>227
Met Asp Thr Leu Phe Arg Leu Val Asn Phe Gln Gln Gln Gln Gln Gln
1 5 10 15
Tyr Gln Pro Asp Pro Ser Leu Asn Ser Thr Thr Thr Leu Thr Thr Ser
20 25 30
Ser Ser Ser Arg Ser Ser Arg Gln Thr Thr Tyr His Tyr Tyr Asn Gln
35 40 45
Gln Glu Glu Asp Glu Glu Cys Phe Asn Asn Phe Tyr Tyr Met Asp His
50 55 60
Asn Asn Asn Asn Asp Glu Asp Leu Ser Ser Ser Ser Ser Lys Gln His
65 70 75 80
Tyr Tyr Thr Tyr Pro Tyr Ala Ser Thr Thr Thr Ile Thr Thr Pro Asn
85 90 95
Thr Thr Tyr Asn Thr Ile Asn Thr Pro Thr Thr Thr Asp Asn Tyr Ser
100 105 110
Phe Ser Pro Ser His Asp Tyr Phe Asn Phe Glu Phe Ser Gly His Ser
115 120 125
Trp Ser Gln Asn Ile Leu Leu Glu Thr Ala Arg Ala Phe Ser Asp Asn
130 135 140
Asn Thr Asn Arg Ile Gln Gln Leu Met Trp Met Leu Asn Glu Leu Ser
145 150 155 160
Thr Pro Tyr Gly Asp Thr Asp Gln Lys Leu Ser Ser Tyr Phe Leu Gln
165 170 175
Ala Leu Phe Ser Arg Met Asn Asp Ala Gly Asp Arg Thr Tyr Lys Thr
180 185 190
Leu Thr Thr Ala Ser Glu Lys Thr Cys Ser Phe Asp Ser Thr Arg Lys
195 200 205
Met Leu Leu Lys Phe Gln Glu Val Ser Pro Trp Thr Thr Phe Gly His
210 215 220
Val Ala Ala Asn Gly Ala Ile Leu Glu Ala Leu Glu Gly Asn Pro Lys
225 230 235 240
Leu His Ile Ile Asp Ile Ser Asn Thr Tyr Cys Thr Gln Trp Pro Thr
245 250 255
Leu Leu Glu Ala Leu Ala Thr Arg Ser Asp Asp Thr Pro His Leu Arg
260 265 270
Leu Thr Thr Val Val Thr Ala Ile Ser Gly Gly Ser Val Gln Lys Val
275 280 285
Met Lys Glu Ile Gly Ser Arg Met Glu Lys Phe Ala Arg Leu Met Gly
290 295 300
Val Pro Phe Lys Phe Lys Ile Ile Phe Ser Asp Leu Arg Glu Leu Asn
305 310 315 320
Leu Cys Asp Leu Asp Ile Lys Glu Asp Glu Ala Leu Ala Ile Asn Cys
325 330 335
Val Asn Ser Leu His Ser Ile Ser Gly Ala Gly Asn His Arg Asp Leu
340 345 350
Phe Ile Ser Leu Leu Arg Gly Leu Glu Pro Arg Val Leu Thr Ile Val
355 360 365
Glu Glu Glu Ala Asp Leu Glu Val Cys Phe Gly Ser Asp Phe Val Glu
370 375 380
Gly Phe Lys Glu Cys Leu Arg Trp Phe Arg Val Tyr Phe Glu Ala Leu
385 390 395 400
Asp Glu Ser Phe Ser Arg Thr Ser Ser Glu Arg Leu Met Leu Glu Arg
405 410 415
Glu Ala Gly Arg Gly Ile Val Asp Leu Val Ala Cys Asp Pro Tyr Glu
420 425 430
Ser Val Glu Arg Arg Glu Thr Ala Ala Arg Trp Arg Arg Arg Leu His
435 440 445
Gly Gly Gly Phe Asn Thr Val Ser Phe Ser Asp Glu Val Cys Asp Asp
450 455 460
Val Arg Ala Leu Leu Arg Arg Tyr Lys Glu Gly Trp Ser Met Thr Ser
465 470 475 480
Ser Asp Gly Asp Thr Gly Ile Phe Leu Ser Trp Lys Asp Lys Pro Val
485 490 495
Val Trp Ala Ser Val Trp Arg Pro
500
<210>228
<211>1539
<212>DNA
<213>马铃薯(Solanum tuberosum)
<400>228
atggatactt tgtttagatt agttaccctt caacaacaac aacaacaaca acaatctgat 60
caatattctt ttaactccag cagaacttca agcagctcta gatcttctaa caaacaaaac 120
acatataatt atcatccaca tcataataat caagacgaag aatgcttcaa ctttttcatg 180
gatgaagatg atttctcttc ttcttcttct aaacacaaca acaactatcc tcctcctcat 240
tacaatcaat atcatcaaca aatttccaac actcccacac cgacaactac aagcagtacc 300
ccgacacaac aatctcaatc tcatcactat gatcatcaat tctccccagc acgtgattta 360
aatctcgaat tcgcttcctc attttccgga aaatgggcga cggacattct tctagaaact 420
tctcgtgcca ttgccgataa gaacagtaca cgtgtccaac aactcatgtg gatgttgaat 480
gagttgagtt ctccatatgg agataccgaa caaaaattgg cttcttattt tcttcaagca 540
ttatttagcc gtatgacgga ttctggcgaa cgatgttatc gtacgttgtt atccgcttcg 600
gataaaacat gttcgttcga gtcaacgagg aaattagttt tgaaatttca ggaagttagc 660
ccttggacga cttttggtca cgttgcatcc aatggtgcaa ttatggaagc cttagaaggt 720
gagtcaaagt tgcatataat tgatattagc aacacttatt gtactcaatg gcctacttta 780
ctagaagcgc tagcaacccg caccgatgaa acaccgcatc tccgcctcac cacggtggtt 840
gccgctagcg gaggtgcagc gtccgtgcaa aaagtgatga aggagattgg taatagaatg 900
gaaaaattcg ctaggcttat gggcgtaccg tttaaattca acgtcattca ccatgcggga 960
aatttatctg agttggatat tggtgcctta gatattaaag gagatgaagc gctagcaatt 1020
aattgtattg gtgcgttaca ttccgttacg ccagctggca atcgaaggga ttatttgata 1080
tcattattta ggaggttaca acctaggatt gttacaattg ttgaagaaga agctgatctt 1140
gatgttggtg ttgatggttt cgattttgtt aaaggttttc aagaatgttt gaaatggctt 1200
agggtttatt ttgaatcatt agacgagagt ttttcgaaaa caagtaacga acgtttgatg 1260
ctagagcgac aagcaggacg ttcgattgtt gatttactag catgcccacc atcggagtca 1320
atggagaggc gggaaacggg ggcaaaatgg tcacatcgta tgcatgcagg agggttcagt 1380
cagcttttat atagtgatga agtttgtgat gatgttagag ctttgttgag aaggtataaa 1440
gatgggtggt cgatgggaca gtgtggggga gattccgccg gaatattctt gtcgtggaag 1500
gaacagccgg tggtgtgggc cagtgcatgg aagccttaa 1539
<210>229
<211>512
<212>PRT
<213>马铃薯
<400>229
Met Asp Thr Leu Phe Arg Leu Val Thr Leu Gln Gln Gln Gln Gln Gln
1 5 10 15
Gln Gln Ser Asp Gln Tyr Ser Phe Asn Ser Ser Arg Thr Ser Ser Ser
20 25 30
Ser Arg Ser Ser Asn Lys Gln Asn Thr Tyr Asn Tyr His Pro His His
35 40 45
Asn Asn Gln Asp Glu Glu Cys Phe Asn Phe Phe Met Asp Glu Asp Asp
50 55 60
Phe Ser Ser Ser Ser Ser Lys His Asn Asn Asn Tyr Pro Pro Pro His
65 70 75 80
Tyr Asn Gln Tyr His Gln Gln Ile Ser Asn Thr Pro Thr Pro Thr Thr
85 90 95
Thr Ser Ser Thr Pro Thr Gln Gln Ser Gln Ser His His Tyr Asp His
100 105 110
Gln Phe Ser Pro Ala Arg Asp Leu Asn Leu Glu Phe Ala Ser Ser Phe
115 120 125
Ser Gly Lys Trp Ala Thr Asp Ile Leu Leu Glu Thr Ser Arg Ala Ile
130 135 140
Ala Asp Lys Asn Ser Thr Arg Val Gln Gln Leu Met Trp Met Leu Asn
145 150 155 160
Glu Leu Ser Ser Pro Tyr Gly Asp Thr Glu Gln Lys Leu Ala Ser Tyr
165 170 175
Phe Leu Gln Ala Leu Phe Ser Arg Met Thr Asp Ser Gly Glu Arg Cys
180 185 190
Tyr Arg Thr Leu Leu Ser Ala Ser Asp Lys Thr Cys Ser Phe Glu Ser
195 200 205
Thr Arg Lys Leu Val Leu Lys Phe Gln Glu Val Ser Pro Trp Thr Thr
210 215 220
Phe Gly His Val Ala Ser Asn Gly Ala Ile Met Glu Ala Leu Glu Gly
225 230 235 240
Glu Ser Lys Leu His Ile Ile Asp Ile Ser Asn Thr Tyr Cys Thr Gln
245 250 255
Trp Pro Thr Leu Leu Glu Ala Leu Ala Thr Arg Thr Asp Glu Thr Pro
260 265 270
His Leu Arg Leu Thr Thr Val Val Ala Ala Ser Gly Gly Ala Ala Ser
275 280 285
Val Gln Lys Val Met Lys Glu Ile Gly Asn Arg Met Glu Lys Phe Ala
290 295 300
Arg Leu Met Gly Val Pro Phe Lys Phe Asn Val Ile His His Ala Gly
305 310 315 320
Asn Leu Ser Glu Leu Asp Ile Gly Ala Leu Asp Ile Lys Gly Asp Glu
325 330 335
Ala Leu Ala Ile Asn Cys Ile Gly Ala Leu His Ser Val Thr Pro Ala
340 345 350
Gly Asn Arg Arg Asp Tyr Leu Ile Ser Leu Phe Arg Arg Leu Gln Pro
355 360 365
Arg Ile Val Thr Ile Val Glu Glu Glu Ala Asp Leu Asp Val Gly Val
370 375 380
Asp Gly Phe Asp Phe Val Lys Gly Phe Gln Glu Cys Leu Lys Trp Leu
385 390 395 400
Arg Val Tyr Phe Glu Ser Leu Asp Glu Ser Phe Ser Lys Thr Ser Asn
405 410 415
Glu Arg Leu Met Leu Glu Arg Gln Ala Gly Arg Ser Ile Val Asp Leu
420 425 430
Leu Ala Cys Pro Pro Ser Glu Ser Met Glu Arg Arg Glu Thr Gly Ala
435 440 445
Lys Trp Ser His Arg Met His Ala Gly Gly Phe Ser Gln Leu Leu Tyr
450 455 460
Ser Asp Glu Val Cys Asp Asp Val Arg Ala Leu Leu Arg Arg Tyr Lys
465 470 475 480
Asp Gly Trp Ser Met Gly Gln Cys Gly Gly Asp Ser Ala Gly Ile Phe
485 490 495
Leu Ser Trp Lys Glu Gln Pro Val Val Trp Ala 8er Ala Trp Lys Pro
500 505 510
<210>230
<211>1485
<212>DNA
<213>巨桉(Eucalyptus grandis)
<400>230
atggatacct tgtttagact agtgagcctt caatcctccg atcagtcctt caactcaagc 60
aggacctcaa gcagctccag atcctcccga caaaaccatc actaccatca ccagccagaa 120
gcggacgaag aatgcctcaa catcttcatg gatgaagaag acttctcctc gtcttcttcc 180
aagcactaca gttatcctta tcaccccgcc accaccagca ccacccctac caccactagt 240
agtactcaca ctccccacac ttatgaccct gccgatgtat ccgtctcgcc cgcccgcgac 300
ctcaacttcg acttctctag caagtgggcc tctgacatcc tcctcgagac ggcccgtgcc 360
atcgtcgaca agaacagcac ccgtgtccag caccttatgt ggatgctcaa tgagctctgc 420
tccccttatg gcgacatcga gcagaagctg gcctcctact tcctccaggc cctgtttagt 480
cgcatgacgg actcgggcga gcggagctac agtgcctggt tggccgcgtc ggacaagacc 540
tgctccttcg agtccacccg gaagatggtg ctcaagttcc aggaggtgag cccctggacc 600
accttcgggc acgtcgcgtg caacggcgca atcatggagg cccttgaggg ggagagcaag 660
ttgcacatag ttgacatcag caacacgtac tgcacccagt ggccaaccct gctcgaggct 720
ctggccaccc ggacggatga gacgccacac ctgcggctca ccaccgtggt ggtgagcaag 780
gcgaacggcg gggcagagac tagtggcgtc gtcgccgtgc agaaggtcat gaaagaaatt 840
gggagccgga tggagaaatt tgcgaggctc atgggtgtgc ccttcaagtt cagcgtcctg 900
taccacagtg gcgacctgtc agagttgaat ttggacgagt tggatatcaa ggaggacgag 960
gcgctggcca tcaactgcgt tggcgcgctg cactcgacaa cgacagtcag caaccgccgg 1020
gactttgtgg tctcatcttt ccgaaggttg caaccgagga taatcacggt tgttgaggag 1080
gaagccgacc tcgacgtggg cgtggatggg atcgaatttg tcaggggttt ccaagaaagc 1140
ctgagatatt ttagggttta cttcgagtcg ttggacgaga gcttccctag aacgagcaac 1200
gagcgcttga tgctcgagcg aggggctggg cgagcggtca tggacctggt ggcatgccca 1260
ccacaccact cggtggagag gcgggagcca gcaagccggt ggtcgaggcg cctgcgagga 1320
ggcgggttta atccttgttt gttcagcgac gaggtatgcg acgacgtgcg tgccttgttg 1380
aggaggtaca aggaagggtg gtcgatgacg ccgtgctctg acgccggaat attcttgacg 1440
tggaaggacc agccggtggt ttgggctagt gcatggaggg cttga 1485
<210>231
<211>494
<212>PRT
<213>巨桉
<400>231
Met Asp Thr Leu Phe Arg Leu Val Ser Leu Gln Ser Ser Asp Gln Ser
1 5 10 15
Phe Asn Ser Ser Arg Thr Ser Ser Ser Ser Arg Ser Ser Arg Gln Asn
20 25 30
His His Tyr His His Gln Pro Glu Ala Asp Glu Glu Cys Leu Asn Ile
35 40 45
Phe Met Asp Glu Glu Asp Phe Ser Ser Ser Ser Ser Lys His Tyr Ser
50 55 60
Tyr Pro Tyr His Pro Ala Thr Thr Ser Thr Thr Pro Thr Thr Thr Ser
65 70 75 80
Ser Thr His Thr Pro His Thr Tyr Asp Pro Ala Asp Val Ser Val Ser
85 90 95
Pro Ala Arg Asp Leu Asn Phe Asp Phe Ser Ser Lys Trp Ala Ser Asp
100 105 110
Ile Leu Leu Glu Thr Ala Arg Ala Ile Val Asp Lys Asn Ser Thr Arg
115 120 125
Val Gln His Leu Met Trp Met Leu Asn Glu Leu Cys Ser Pro Tyr Gly
130 135 140
Asp Ile Glu Gln Lys Leu Ala Ser Tyr Phe Leu Gln Ala Leu Phe Ser
145 150 155 160
Arg Met Thr Asp Ser Gly Glu Arg Ser Tyr Ser Ala Trp Leu Ala Ala
165 170 175
Ser Asp Lys Thr Cys Ser Phe Glu Ser Thr Arg Lys Met Val Leu Lys
180 185 190
Phe Gln Glu Val Ser Pro Trp Thr Thr Phe Gly His Val Ala Cys Asn
195 200 205
Gly Ala Ile Met Glu Ala Leu Glu Gly Glu Ser Lys Leu His Ile Val
210 215 220
Asp Ile Ser Asn Thr Tyr Cys Thr Gln Trp Pro Thr Leu Leu Glu Ala
225 230 235 240
Leu Ala Thr Arg Thr Asp Glu Thr Pro His Leu Arg Leu Thr Thr Val
245 250 255
Val Val Ser Lys Ala Asn Gly Gly Ala Glu Thr Ser Gly Val Val Ala
260 265 270
Val Gln Lys Val Met Lys Glu Ile Gly Ser Arg Met Glu Lys Phe Ala
275 280 285
Arg Leu Met Gly Val Pro Phe Lys Phe Ser Val Leu Tyr His Ser Gly
290 295 300
Asp Leu Ser Glu Leu Asn Leu Asp Glu Leu Asp Ile Lys Glu Asp Glu
305 310 315 320
Ala Leu Ala Ile Asn Cys Val Gly Ala Leu His Ser Thr Thr Thr Val
325 330 335
Ser Asn Arg Arg Asp Phe Val Val Ser Ser Phe Arg Arg Leu Gln Pro
340 345 350
Arg Ile Ile Thr Val Val Glu Glu Glu Ala Asp Leu Asp Val Gly Val
355 360 365
Asp Gly Ile Glu Phe Val Arg Gly Phe Gln Glu Ser Leu Arg Tyr Phe
370 375 380
Arg Val Tyr Phe Glu Ser Leu Asp Glu Ser Phe Pro Arg Thr Ser Asn
385 390 395 400
Glu Arg Leu Met Leu Glu Arg Gly Ala Gly Arg Ala Val Met Asp Leu
405 410 415
Val Ala Cys Pro Pro His His Ser Val Glu Arg Arg Glu Pro Ala Ser
420 425 430
Arg Trp Ser Arg Arg Leu Arg Gly Gly Gly Phe Asn Pro Cys Leu Phe
435 440 445
Ser Asp Glu Val Cys Asp Asp Val Arg Ala Leu Leu Arg Arg Tyr Lys
450 455 460
Glu Gly Trp Ser Met Thr Pro Cys Ser Asp Ala Gly Ile Phe Leu Thr
465 470 475 480
Trp Lys Asp Gln Pro Val Val Trp Ala Ser Ala Trp Arg Ala
485 490
<210>232
<211>1485
<212>DNA
<213>葡萄
<220>
<221>misc_feature
<222>(270)..(270)
<223>n是a、c、g或t
<400>232
atggatacct tgtttagact agtcagtctt caatccgatc aatcctacaa ctccagtaga 60
acttctagca gctctagatc ttctagacaa aaccatcacc accaccacca agaagacgaa 120
gaatgcttca actttttcat ggatgaagaa gacttctctt cttcttcttc caagcactac 180
tatccttatc agcaacaaac ccatccttct tcaccaccac ccccaccacc accaacacca 240
gcactcctac tcatcatgcc tttgagcccn accgaattct ctttctcccc tgcccgtgat 300
ctccacctag aattcaactc ttctgcttcc ggcaagtggg cttccgaaat cctcctggag 360
acagcacgag ccatcgctga taagaacagc gctcgcgttc aacaactcat gtggatgctc 420
aacgagctta gctctcctta tggcgacacc gatcagaagc tcgctgctta cttccttcaa 480
gccttgttta gccgcatgac cgactcgggt gagaggtgtt accggacttt aatatccgca 540
tcggagaaga cttgctcatt cgaatccacc agaaaaatgg tattgaaatt tcaagaggtg 600
agcccttgga ccacgtttgg acatgtagct tgtaatggtg caatcatgga agctcttgaa 660
ggcgagagca agctccatat aattgatata agcaacacct actgcactca gtggcccacc 720
ttgctcgaag ccctagccac ccgcactgac gagacccctc acctccgtct caccaccgtc 780
gtcacaagca aagccggcac cggagggatg gcaccagtgc aaaaactgat gaaagagatt 840
ggaaacagaa tggaaaagtt cgctaggctc atgggtgtgc ccttcaaatt caacgtactg 900
caccactccg gcgacctatc ccatctgaat ttagcagaat tggacatcaa agacgacgag 960
gcactcgccg taaactgcgt cggcgcgttg cactcagtca ccgcagttgg taaccgccga 1020
gacatcgtcg tatcatcttt ccggagattg catccgagga tcatcaccgt cgtggaagaa 1080
gaagctgatc tcgatgtggg cgtggatggg ttcgacttcg tcaaaggttt tcaggaatgc 1140
ttaagatggt ttagggttta cttggagtca ttagacgaga gcttcccacg cactagcaac 1200
gagcgcctca tgctcgaacg cgccgccgga cgmgcaatcg tcgacctggt ggcttgcccg 1260
ccgtcggagt cgatcgaacg gcgcgagacg gccacgcgct ggtckcagcg gctccatgca 1320
agcgggttct ctccggtgtc gtttagcgat gaagtatgcg atgacgtacg tgctctcctg 1380
aggagataca aggagggttg gtcaatgaca cagtcctccg acgccggaat attcctctca 1440
tggaaagacc agccggtggt gtggaccagt gcatggaaac cttga 1485
<210>233
<211>494
<212>PRT
<213>葡萄
<400>233
Met Asp Thr Leu Phe Arg Leu Val Ser Leu Gln Ser Asp Gln Ser Tyr
1 5 10 15
Asn Ser Ser Arg Thr Ser Ser Ser Ser Arg Ser Ser Arg Gln Asn His
20 25 30
His His His His Gln Glu Asp Glu Glu Cys Phe Asn Phe Phe Met Asp
35 40 45
Glu Glu Asp Phe Ser Ser Ser Ser Ser Lys His Tyr Tyr Pro Tyr Gln
50 55 60
Gln Gln Thr His Pro Ser Ser Pro Pro Pro Pro Pro Pro Pro Thr Pro
65 70 75 80
Ala Leu Leu Leu Ile Met Pro Leu Ser Pro Thr Glu Phe Ser Phe Ser
85 90 95
Pro Ala Arg Asp Leu His Leu Glu Phe Asn Ser Ser Ala Ser Gly Lys
100 105 110
Trp Ala Ser Glu Ile Leu Leu Glu Thr Ala Arg Ala Ile Ala Asp Lys
115 120 125
Asn Ser Ala Arg Val Gln Gln Leu Met Trp Met Leu Asn Glu Leu Ser
130 135 140
Ser Pro Tyr Gly Asp Thr Asp Gln Lys Leu Ala Ala Tyr Phe Leu Gln
145 150 155 160
Ala Leu Phe Ser Arg Met Thr Asp Ser Gly Glu Arg Cys Tyr Arg Thr
165 170 175
Leu Ile Ser Ala Ser Glu Lys Thr Cys Ser Phe Glu Ser Thr Arg Lys
180 185 190
Met Val Leu Lys Phe Gln Glu Val Ser Pro Trp Thr Thr Phe Gly His
195 200 205
Val Ala Cys Asn Gly Ala Ile Met Glu Ala Leu Glu Gly Glu Ser Lys
210 215 220
Leu His Ile Ile Asp Ile Ser Asn Thr Tyr Cys Thr Gln Trp Pro Thr
225 230 235 240
Leu Leu Glu Ala Leu Ala Thr Arg Thr Asp Glu Thr Pro His Leu Arg
245 250 255
Leu Thr Thr Val Val Thr Ser Lys Ala Gly Thr Gly Gly Met Ala Pro
260 265 270
Val Gln Lys Leu Met Lys Glu Ile Gly Asn Arg Met Glu Lys Phe Ala
275 280 285
Arg Leu Met Gly Val Pro Phe Lys Phe Asn Val Leu His His Ser Gly
290 295 300
Asp Leu Ser His Leu Asn Leu Ala Glu Leu Asp Ile Lys Asp Asp Glu
305 310 315 320
Ala Leu Ala Val Asn Cys Val Gly Ala Leu His Ser Val Thr Ala Val
325 330 335
Gly Asn Arg Arg Asp Ile Val Val Ser Ser Phe Arg Arg Leu His Pro
340 345 350
Arg Ile Ile Thr Val Val Glu Glu Glu Ala Asp Leu Asp Val Gly Val
355 360 365
Asp Gly Phe Asp Phe Val Lys Gly Phe Gln Glu Cys Leu Arg Trp Phe
370 375 380
Arg Val Tyr Leu Glu Ser Leu Asp Glu Ser Phe Pro Arg Thr Ser Asn
385 390 395 400
Glu Arg Leu Met Leu Glu Arg Ala Ala Gly Arg Ala Ile Val Asp Leu
405 410 415
Val Ala Cys Pro Pro Ser Glu Ser Ile Glu Arg Arg Glu Thr Ala Thr
420 425 430
Arg Trp Ser Gln Arg Leu His Ala Ser Gly Phe Ser Pro Val Ser Phe
435 440 445
Ser Asp Glu Val Cys Asp Asp Val Arg Ala Leu Leu Arg Arg Tyr Lys
450 455 460
Glu Gly Trp Ser Met Thr Gln Ser Ser Asp Ala Gly Ile Phe Leu Set
465 470 475 480
Trp Lys Asp Gln Pro Val Val Trp Thr Ser Ala Trp Lys Pro
485 490
<210>234
<211>1533
<212>DNA
<213>毛果杨
<400>234
atggatacct tgtttaggct agttagtctc caacaacaat ctgaacaatc tttcaactct 60
actagcagaa cctctagtag ctctagatca tcaagacaaa acaacaacca ccaccatcat 120
cactatcaac aagaagacga agaatgcttc aactttttca tggatgagga agacttctct 180
tcatcttctt ctaagcacta ctatcctcct tatcaccaca accaacaaca acaacatcaa 240
caccaaacca ccaccaccac tcctaccact actaccacca acactagcac cccttctact 300
caccatgtcc ttgattccgc tgacttctct ttctcccctt ctcatgacct aaactttgaa 360
ttttccggca agtgggtcac cgatatcctc cttgaatctg cacatgccat cgcggataaa 420
aacagcgctc gtctccagca attgatgtgg atgcttaatg agcttggttc accttatggt 480
gacacagagc aaaaacttgc ttcttatttt ctccaagctt tgtttagccg catgaacgac 540
tccggcgaga gatgctaccg tactttagct tcagcatcag agaaaacttg ctcttttgat 600
tccacaagga aaatggtatt aaagtttcaa gaggtgagtc cttggactac ttttggtcac 660
gtatcttgta atggcgcaat tatggaagca tttgaaggtg aaagcaaatt gcatattatt 720
gatattagta acacatattg tacccaatgg cctactttgc tcgaagccct agcaactcgc 780
actgatgaga caccacactt gaagttaacc accgtagtgg ctagcaaaag tagtggtaat 840
aatattggtt taactagtac aggaggttta gcttcagttc ataaggtaat gaaagaaatt 900
ggcaacagaa tggaaaaatt tgccaggctt atgggagtcc catttaagtt taatgttatc 960
caccatgctg gtgatttatg tgacctaaac ttagctgaat tggatgttaa agatgatgag 1020
gctcttgcta tcaactgtgt tggtgcttta cactcaatca ctccagcttc tcgtcgccga 1080
gattatgtta tatctagttt tagaacattg caaccaagaa tcattactgt tgttgaagaa 1140
gaagctgatc ttgatggtct ggattttgtc aagggttttc aagaatgttt aagatggttt 1200
agggtttact ttgaatcatt ggatgagagc tttccaagaa ccagtaacga acagttgatg 1260
cttgaaagag cagcaggccg cgctatcgtt gacttagtgg catgtcctcc atctgattcg 1320
atcgaaaggc gggaaacagc cacgcgctgg tctggacgcc tccattcatg tggttttagc 1380
ccgataattt tcagtgatga ggtttgtgat gatgtacgcg ccttattgag gaggtataag 1440
gagggttggt caatgacaca gtgcggggat gccggaatat tcttgtgctg gaaggaacag 1500
ccggtggtgt gggctagtgc atggaggccc tga 1533
<210>235
<211>510
<212>PRT
<213>毛果杨
<400>235
Met Asp Thr Leu Phe Arg Leu Val Ser Leu Gln Gln Gln Ser Glu Gln
1 5 10 15
Ser Phe Asn Ser Thr Ser Arg Thr Ser Ser Ser Ser Arg Ser Ser Arg
20 25 30
Gln Asn Asn Asn His His His His His Tyr Gln Gln Glu Asp Glu Glu
35 40 45
Cys Phe Asn Phe Phe Met Asp Glu Glu Asp Phe Ser Ser Ser Ser Ser
50 55 60
Lys His Tyr Tyr Pro Pro Tyr His His Asn Gln Gln Gln Gln His Gln
65 70 75 80
His Gln Thr Thr Thr Thr Thr Pro Thr Thr Thr Thr Thr Asn Thr Ser
85 90 95
Thr Pro Ser Thr His His Val Leu Asp Ser Ala Asp Phe Ser Phe Ser
100 105 110
Pro Ser His Asp Leu Asn Phe Glu Phe Ser Gly Lys Trp Val Thr Asp
115 120 125
Ile Leu Leu Glu Ser Ala His Ala Ile Ala Asp Lys Asn Ser Ala Arg
130 135 140
Leu Gln Gln Leu Met Trp Met Leu Asn Glu Leu Gly Ser Pro Tyr Gly
145 150 155 160
Asp Thr Glu Gln Lys Leu Ala Ser Tyr Phe Leu Gln Ala Leu Phe Ser
165 170 175
Arg Met Asn Asp Ser Gly Glu Arg Cys Tyr Arg Thr Leu Ala Ser Ala
180 185 190
Ser Glu Lys Thr Cys Ser Phe Asp Ser Thr Arg Lys Met Val Leu Lys
195 200 205
Phe Gln Glu Val Ser Pro Trp Thr Thr Phe Gly His Val Ser Cys Asn
210 215 220
Gly Ala Ile Met Glu Ala Phe Glu Gly Glu Ser Lys Leu His Ile Ile
225 230 235 240
Asp Ile Ser Asn Thr Tyr Cys Thr Gln Trp Pro Thr Leu Leu Glu Ala
245 250 255
Leu Ala Thr Arg Thr Asp Glu Thr Pro His Leu Lys Leu Thr Thr Val
260 265 270
Val Ala Ser Lys Ser Ser Gly Asn Asn Ile Gly Leu Thr Ser Thr Gly
275 280 285
Gly Leu Ala Ser Val His Lys Val Met Lys Glu Ile Gly Asn Arg Met
290 295 300
Glu Lys Phe Ala Arg Leu Met Gly Val Pro Phe Lys Phe Asn Val Ile
305 310 315 320
His His Ala Gly Asp Leu Cys Asp Leu Asn Leu Ala Glu Leu Asp Val
325 330 335
Lys Asp Asp Glu Ala Leu Ala Ile Asn Cys Val Gly Ala Leu His Ser
340 345 350
Ile Thr Pro Ala Ser Arg Arg Arg Asp Tyr Val Ile Ser Ser Phe Arg
355 360 365
Thr Leu Gln Pro Arg Ile Ile Thr Val Val Glu Glu Glu Ala Asp Leu
370 375 380
Asp Gly Leu Asp Phe Val Lys Gly Phe Gln Glu Cys Leu Arg Trp Phe
385 390 395 400
Arg Val Tyr Phe Glu Ser Leu Asp Glu Ser Phe Pro Arg Thr Ser Asn
405 410 415
Glu Gln Leu Met Leu Glu Arg Ala Ala Gly Arg Ala Ile Val Asp Leu
420 425 430
Val Ala Cys Pro Pro Set Asp Ser Ile Glu Arg Arg Glu Thr Ala Thr
435 440 445
Arg Trp Ser Gly Arg Leu His Set Cys Gly Phe Ser Pro Ile Ile Phe
450 455 460
Ser Asp Glu Val Cys Asp Asp Val Arg Ala Leu Leu Arg Arg Tyr Lys
465 470 475 480
Glu Gly Trp Ser Met Thr Gln Cys Gly Asp Ala Gly Ile Phe Leu Cys
485 490 495
Trp Lys Glu Gln Pro Val Val Trp Ala Ser Ala Trp Arg Pro
500 505 510
<210>236
<211>1539
<212>DNA
<213>蓖麻(Ricinus communis)
<400>236
atggatacgt tgtttaggct agttagtctt caacaacagc aatctgatca atctttcaat 60
aactctacta gcagaacctc tagcagctct agatcctcaa gacaaaacaa ccaccaccat 120
catcatcatc agtaccaaca agaagacgaa gaatgcttca actttttcat ggatgaagaa 180
gacttctctt cgtcttcttc taaacactac tatccttatc accatcaaca acagcaacaa 240
cacaccaccg ccaccactac tactactact gtgaccactc ctaacactac cactagtact 300
cttgaatcca ctgaccactt ctctttctct ccttcccatg acctaaactt tgacttctcc 360
agcaggtggg caaccgatat cctcctcgaa actgctcgtg ctattgctga taaaaacagt 420
gctcgtgttc agcaactcat gtggatgctt aatgaacttg gctcgcctta tggggacata 480
gacaaaaagc ttgcatcata ctttctacaa gccttgttca gccggatgac tgattccggt 540
gagagatgtt accgtacttt agcctctgca tcggataaaa cttgctcttt cgagtcaaca 600
agaaagatgg tattgaagtt tcaagaggtg agtccttgga ctacttttgg tcacgtatct 660
tgtaatggtg caataatgga agcatttgaa ggtgaaagta aattgcatat tattgatatt 720
agtaacacat attgcactca gtggccaacg ttgctagaag ccctagcaac tcgcaccgat 780
gagacacccc acttgaggct aaccaccatt gtggctagta aaacaaatgg tggtgggggc 840
ggcggcggtg gcaattgtgg tttagtttca gcccagaaag taatgaaaga aattggaagc 900
agaatggaaa aatttgctag gcttatggga gtaccattta aattcaatgt tatacaccat 960
gctggtgact tgtgtgacct gaacttatct gaattggata ttaaagaaga tgaagcttta 1020
gccatcaatt gtatagggtc actgcattca gttacaacag ttgctaatcg tagagattat 1080
ataatatcaa actttagaag attacaacca agaatcatca ctattgttga agaagaagct 1140
gatcttgatg tgggtattga tgggttagac tttgtaagag gttttcaaga atgtttaaga 1200
tggtttaggg tttactttga gtcattagaa gaaagttttt caagaacaag caatgaaaga 1260
ttgatgctag agagaagtgc tggacgtgca atagttgact tggttgcatg tcagccatca 1320
gagtcaattg aacggcggga aaaggcgaca cggtggtcct cacggttgca tgcatgtgga 1380
tatagcccta ttctgtttag tgatgaggta tgcgatgatg tacgcgccct tttgaggagg 1440
tataaggaag gctggtcaat gacacagagc ggccacgccg gaatattcct atgctggaag 1500
gaacagccgg tcgtgtgggc tagtgcatgg aaaccttga 1539
<210>237
<211>512
<212>PRT
<213>蓖麻
<400>237
Met Asp Thr Leu Phe Arg Leu Val Ser Leu Gln Gln Gln Gln Ser Asp
1 5 10 15
Gln Ser Phe Asn Asn Ser Thr Ser Arg Thr Ser Ser Ser Ser Arg Ser
20 25 30
Ser Arg Gln Asn Asn His His His His His His Gln Tyr Gln Gln Glu
35 40 45
Asp Glu Glu Cys Phe Asn Phe Phe Met Asp Glu Glu Asp Phe Ser Ser
50 55 60
Ser Ser Ser Lys His Tyr Tyr Pro Tyr His His Gln Gln Gln Gln Gln
65 70 75 80
His Thr Thr Ala Thr Thr Thr Thr Thr Thr Val Thr Thr Pro Asn Thr
85 90 95
Thr Thr Ser Thr Leu Glu Ser Thr Asp His Phe Ser Phe Ser Pro Ser
100 105 110
His Asp Leu Asn Phe Asp Phe Ser Ser Arg Trp Ala Thr Asp Ile Leu
115 120 125
Leu Glu Thr Ala Arg Ala Ile Ala Asp Lys Asn Ser Ala Arg Val Gln
130 135 140
Gln Leu Met Trp Met Leu Asn Glu Leu Gly Ser Pro Tyr Gly Asp Ile
145 150 155 160
Asp Lys Lys Leu Ala Ser Tyr Phe Leu Gln Ala Leu Phe Ser Arg Met
165 170 175
Thr Asp Ser Gly Glu Arg Cys Tyr Arg Thr Leu Ala Ser Ala Ser Asp
180 185 190
Lys Thr Cys Ser Phe Glu Ser Thr Arg Lys Met Val Leu Lys Phe Gln
195 200 205
Glu Val Ser Pro Trp Thr Thr Phe Gly His Val Ser Cys Asn Gly Ala
210 215 220
Ile Met Glu Ala Phe Glu Gly Glu Ser Lys Leu His Ile Ile Asp Ile
225 230 235 240
Ser Asn Thr Tyr Cys Thr Gln Trp Pro Thr Leu Leu Glu Ala Leu Ala
245 250 255
Thr Arg Thr Asp Glu Thr Pro His Leu Arg Leu Thr Thr Ile Val Ala
260 265 270
Ser Lys Thr Asn Gly Gly Gly Gly Gly Gly Gly Gly Asn Cys Gly Leu
275 280 285
Val Ser Ala Gln Lys Val Met Lys Glu Ile Gly Ser Arg Met Glu Lys
290 295 300
Phe Ala Arg Leu Met Gly Val Pro Phe Lys Phe Asn Val Ile His His
305 310 315 320
Ala Gly Asp Leu Cys Asp Leu Asn Leu Ser Glu Leu Asp Ile Lys Glu
325 330 335
Asp Glu Ala Leu Ala Ile Asn Cys Ile Gly Ser Leu His Ser Val Thr
340 345 350
Thr Val Ala Asn Arg Arg Asp Tyr Ile Ile Ser Asn Phe Arg Arg Leu
355 360 365
Gln Pro Arg Ile Ile Thr Ile Val Glu Glu Glu Ala Asp Leu Asp Val
370 375 380
Gly Ile Asp Gly Leu Asp Phe Val Arg Gly Phe Gln Glu Cys Leu Arg
385 390 395 400
Trp Phe Arg Val Tyr Phe Glu Ser Leu Glu Glu Ser Phe Ser Arg Thr
405 410 415
Ser Asn Glu Arg Leu Met Leu Glu Arg Ser Ala Gly Arg Ala Ile Val
420 425 430
Asp Leu Val Ala Cys Gln Pro Ser Glu Ser Ile Glu Arg Arg Glu Lys
435 440 445
Ala Thr Arg Trp Ser Ser Arg Leu His Ala Cys Gly Tyr Ser Pro Ile
450 455 460
Leu Phe Ser Asp Glu Val Cys Asp Asp Val Arg Ala Leu Leu Arg Arg
465 470 475 480
Tyr Lys Glu Gly Trp Ser Met Thr Gln Ser Gly His Ala Gly Ile Phe
485 490 495
Leu Cys Trp Lys Glu Gln Pro Val Val Trp Ala Ser Ala Trp Lys Pro
500 505 510
<210>238
<211>1512
<212>DNA
<213>大豆
<400>238
atggatacct tgtttagact agtaagtttt catcaacagc aacaaccaca acaacaacct 60
gatccatccc tcaactccac caccagcaga acctccagca gctccagatc ctccagacaa 120
aactactacc cttattccca tcaagaagaa gaagaatgct tcaactttta catggatgaa 180
gaagacttat cctcgtcttc ttccaaacac tactaccacc cttatcaacc ccatcatcat 240
caacaacaca acgtatccaa catttctaca aacaccttca gcaccacccc taacactgat 300
tactcctact cctactcctt ctctcccacg caacctgttc aagacttcaa cttcgaattc 360
tcctccccca actggtccca caacctcctc ctcgaatccg cacgcgccgt cgccgacaac 420
aactccacgc gcctccacca cctcctctgg atgctcaacg agctcagctc cccctacggc 480
gacaccgaac aaaaacttgc tgcgtatttc ctccgagcct tgttcagccg cgtcaccgaa 540
gctggcgacc gaacctaccg aagcttagct tcggcgtctg agaaaacatg ctcatttgaa 600
tccacaagaa aaacggtcct gaaattccaa gaagttagcc cttggactac cttcggacac 660
gtggcatcca atggcgccat cttggaagcc ttggagggaa actccaaatt gcatatactt 720
gacatcagca acacttattg cacccagtgg ccaatgcttc tggaagcatt ggccactcgg 780
agcgaagaaa ctccacacct atgtttaaca acaattgtta ctggttctag aatcggtaac 840
aacgtccagc gcgtgatgaa ggaaatcgga acgcgaatgg agaaattcgc cagactcatg 900
ggcgttcctt ttaagttcaa cgtcgttcat cactacggcg atctatccga gttcaatttc 960
tcagagcttg atatcaaaga cgatgaggct ttggctgtga attgtgtgaa ctcgctgcat 1020
tcggtttccg cgctaggaaa caaccgcgac gcgttgatat cggcgttgca agcgttgcag 1080
ccgcggattg tgacggtggt ggaggaggag gctgatttgg acgttggaat cgacggttac 1140
gagttcgtaa aaggctttga agaaagtttg aggtggttta gggtttactt tgaggctttg 1200
gatgagagtt ttgtgaagac gagcaacgag aggctgatgc tggagcgcgc ggcggggagg 1260
gcggtggtgg acctggtggc gtgctcgccg gcggattccg tggagcgccg cgagacggcg 1320
gcgcggtggg cggcgaggct tcacaacgga gggctaaacg cggcgccgtt tagcgacgaa 1380
gtttgcgacg acgtgagggc gctgttgagg aggtacaagg aagggtggtc gatggcggcg 1440
tgctccgacg ccggaatatt cctctcgtgg aaggacacgc cggtggtgtg ggccagtgca 1500
tggaggccat aa 1512
<210>239
<211>503
<212>PRT
<213>大豆
<400>239
Met Asp Thr Leu Phe Arg Leu Val Ser Phe His Gln Gln Gln Gln Pro
1 5 10 15
Gln Gln Gln Pro Asp Pro Ser Leu Asn Ser Thr Thr Ser Arg Thr Ser
20 25 30
Ser Ser Ser Arg Ser Ser Arg Gln Asn Tyr Tyr Pro Tyr Ser His Gln
35 40 45
Glu Glu Glu Glu Cys Phe Asn Phe Tyr Met Asp Glu Glu Asp Leu Ser
50 55 60
Ser Ser Ser Ser Lys His Tyr Tyr His Pro Tyr Gln Pro His His His
65 70 75 80
Gln Gln His Asn Val Ser Asn Ile Ser Thr Asn Thr Phe Ser Thr Thr
85 90 95
Pro Asn Thr Asp Tyr Ser Tyr Ser Tyr Ser Phe Ser Pro Thr Gln Pro
100 105 110
Val Gln Asp Phe Asn Phe Glu Phe Ser Ser Pro Asn Trp Ser His Asn
115 120 125
Leu Leu Leu Glu Ser Ala Arg Ala Val Ala Asp Asn Asn Ser Thr Arg
130 135 140
Leu His His Leu Leu Trp Met Leu Asn Glu Leu Ser Ser Pro Tyr Gly
145 150 155 160
Asp Thr Glu Gln Lys Leu Ala Ala Tyr Phe Leu Arg Ala Leu Phe Ser
165 170 175
Arg Val Thr Glu Ala Gly Asp Arg Thr Tyr Arg Ser Leu Ala Ser Ala
180 185 190
Ser Glu Lys Thr Cys Ser Phe Glu Ser Thr Arg Lys Thr Val Leu Lys
195 200 205
Phe Gln Glu Val Ser Pro Trp Thr Thr Phe Gly His Val Ala Ser Asn
210 215 220
Gly Ala Ile Leu Glu Ala Leu Glu Gly Asn Ser Lys Leu His Ile Leu
225 230 235 240
Asp Ile Ser Asn Thr Tyr Cys Thr Gln Trp Pro Met Leu Leu Glu Ala
245 250 255
Leu Ala Thr Arg Ser Glu Glu Thr Pro His Leu Cys Leu Thr Thr Ile
260 265 270
Val Thr Gly Ser Arg Ile Gly Asn Asn Val Gln Arg Val Met Lys Glu
275 280 285
Ile Gly Thr Arg Met Glu Lys Phe Ala Arg Leu Met Gly Val Pro Phe
290 295 300
Lys Phe Asn Val Val His His Tyr Gly Asp Leu Ser Glu Phe Asn Phe
305 310 315 320
Ser Glu Leu Asp Ile Lys Asp Asp Glu Ala Leu Ala Val Asn Cys Val
325 330 335
Asn Ser Leu His Ser Val Ser Ala Leu Gly Asn Asn Arg Asp Ala Leu
340 345 350
Ile Ser Ala Leu Gln Ala Leu Gln Pro Arg Ile Val Thr Val Val Glu
355 360 365
Glu Glu Ala Asp Leu Asp Val Gly Ile Asp Gly Tyr Glu Phe Val Lys
370 375 380
Gly Phe Glu Glu Ser Leu Arg Trp Phe Arg Val Tyr Phe Glu Ala Leu
385 390 395 400
Asp Glu Ser Phe Val Lys Thr Ser Asn Glu Arg Leu Met Leu Glu Arg
405 410 415
Ala Ala Gly Arg Ala Val Val Asp Leu Val Ala Cys Ser Pro Ala Asp
420 425 430
Ser Val Glu Arg Arg Glu Thr Ala Ala Arg Trp Ala Ala Arg Leu His
435 440 445
Asn Gly Gly Leu Asn Ala Ala Pro Phe Ser Asp Glu Val Cys Asp Asp
450 455 460
Val Arg Ala Leu Leu Arg Arg Tyr Lys Glu Gly Trp Ser Met Ala Ala
465 470 475 480
Cys Ser Asp Ala Gly Ile Phe Leu Ser Trp Lys Asp Thr Pro Val Val
485 490 495
Trp Ala Ser Ala Trp Arg Pro
500
<210>240
<211>1476
<212>DNA
<213>蒺藜苜蓿
<400>240
atggatacat tgtttagact tgtcagtttt caaccacacc aacaacaaga agatcaatct 60
ctcaactcca ccaccagcag aacaacaaca tcaagtagct ctcgatcctc aaaacaaaac 120
tatcaccaat attacaatcc acaagaagtt gaagaagaat gcttcaactt tttcatggat 180
gaagaagacc tttcctcatc ttcttccaaa cactactatc cttataatca acctcaaacc 240
tcaaatacta ttactacaaa cacccccaca acatcactca caccccctcc accaacagct 300
gatttcacct tcgaattaac cggaaggtgg gccaacaaca tcctcctcga aacagcacga 360
gccatagcag aaaaaaacag cactcgttta caacaactca tgtggatgct taacgaacta 420
agctcccctt atggtgacat agaacaaaaa ctagctgcat attttctcca agccttattc 480
tctcgaatga ccgaagccgg tacccgaacc ttccgaaccc tagcttcagc atcagaaaaa 540
acttgctctt tcgaatcaac aagaaaaacg gttctaaaat tccaagaagt tagtccgtgg 600
acaaccttcg gccatgtcgc atgcaatggc gccattttag aagccttcga aggtgattcc 660
aagctgcaca taattgatat tagtaacact tattgcactc agtggccaac tctttttgaa 720
gcgttggcca ctcgtgctga cgatactcct cacctccgtc taactaccat cgtcactgct 780
ggtggttctg ttcaaaaagt tatgaaggaa attggtgcta gaatggaaaa attcgctaga 840
ctcatgggag ttccatttaa attcaacgtt attcatcact ccggcgatct ttctgatttg 900
aattttttgg atttagatat taaagaagat gaagctttag ctgttaattg tgttaatgct 960
ttgcattcag taacagttgg caatggtaat ggtaacggta ataaccgtcg tgactcgttg 1020
atagcttcgt tgatcgcgtt gcggcctagg atagtaacaa tggttgaaga ggaggctgat 1080
ttaaattttg gtaatgaagg atatgaattt gtgaatggtt ttgaagagtg tttaaggtgg 1140
tttagggttt actttgaagc attagaagag agttttccga agacgagtaa cgagcgtttg 1200
atgcttgaaa gggaagcagg gagagggata gttgatttgg tggcatgtgc tccggcggag 1260
tcgattgaac ggagagaaac tgcggtgagg tggtcacggc gtttgcatgg tcgagggttt 1320
aatacggtgg cgtttagtga ggaagtttgt gatgatgtta gggctttgtt gaggaggtat 1380
aaggaagggt ggtcgatgat acggtgtaat gatgccggaa tattcttgac gtggaaggaa 1440
caaccggtgg tgtgggccag tgcatggagg ccttga 1476
<210>241
<211>491
<212>PRT
<213>蒺藜苜蓿
<400>241
Met Asp Thr Leu Phe Arg Leu Val Ser Phe Gln Pro His Gln Gln Gln
1 5 10 15
Glu Asp Gln Ser Leu Asn Ser Thr Thr Ser Arg Thr Thr Thr Ser Ser
20 25 30
Ser Ser Arg Ser Ser Lys Gln Asn Tyr His Gln Tyr Tyr Asn Pro Gln
35 40 45
Glu Val Glu Glu Glu Cys Phe Asn Phe Phe Met Asp Glu Glu Asp Leu
50 55 60
Ser Ser Ser Ser Ser Lys His Tyr Tyr Pro Tyr Asn Gln Pro Gln Thr
65 70 75 80
Ser Asn Thr Ile Thr Thr Asn Thr Pro Thr Thr Ser Leu Thr Pro Pro
85 90 95
Pro Pro Thr Ala Asp Phe Thr Phe Glu Leu Thr Gly Arg Trp Ala Asn
100 105 110
Asn Ile Leu Leu Glu Thr Ala Arg Ala Ile Ala Glu Lys Asn Ser Thr
115 120 125
Arg Leu Gln Gln Leu Met Trp Met Leu Asn Glu Leu Ser Ser Pro Tyr
130 135 140
Gly Asp Ile Glu Gln Lys Leu Ala Ala Tyr Phe Leu Gln Ala Leu Phe
145 150 155 160
Ser Arg Met Thr Glu Ala Gly Thr Arg Thr Phe Arg Thr Leu Ala Ser
165 170 175
Ala Ser Glu Lys Thr Cys Ser Phe Glu Ser Thr Arg Lys Thr Val Leu
180 185 190
Lys Phe Gln Glu Val Ser Pro Trp Thr Thr Phe Gly His Val Ala Cys
195 200 205
Asn Gly Ala Ile Leu Glu Ala Phe Glu Gly Asp Ser Lys Leu His Ile
210 215 220
Ile Asp Ile Ser Asn Thr Tyr Cys Thr Gln Trp Pro Thr Leu Phe Glu
225 230 235 240
Ala Leu Ala Thr Arg Ala Asp Asp Thr Pro His Leu Arg Leu Thr Thr
245 250 255
Ile Val Thr Ala Gly Gly Ser Val Gln Lys Val Met Lys Glu Ile Gly
260 265 270
Ala Arg Met Glu Lys Phe Ala Arg Leu Met Gly Val Pro Phe Lys Phe
275 280 285
Asn Val Ile His His Ser Gly Asp Leu Ser Asp Leu Asn Phe Leu Asp
290 295 300
Leu Asp Ile Lys Glu Asp Glu Ala Leu Ala Val Asn Cys Val Asn Ala
305 310 315 320
Leu His Ser Val Thr Val Gly Asn Gly Asn Gly Asn Gly Asn Asn Arg
325 330 335
Arg Asp Ser Leu Ile Ala Ser Leu Ile Ala Leu Arg Pro Arg Ile Val
340 345 350
Thr Met Val Glu Glu Glu Ala Asp Leu Asn Phe Gly Asn Glu Gly Tyr
355 360 365
Glu Phe Val Asn Gly Phe Glu Glu Cys Leu Arg Trp Phe Arg Val Tyr
370 375 380
Phe Glu Ala Leu Glu Glu Ser Phe Pro Lys Thr Ser Asn Glu Arg Leu
385 390 395 400
Met Leu Glu Arg Glu Ala Gly Arg Gly Ile Val Asp Leu Val Ala Cys
405 410 415
Ala Pro Ala Glu Ser Ile Glu Arg Arg Glu Thr Ala Val Arg Trp Ser
420 425 430
Arg Arg Leu His Gly Arg Gly Phe Asn Thr Val Ala Phe Ser Glu Glu
435 440 445
Val Cys Asp Asp Val Arg Ala Leu Leu Arg Arg Tyr Lys Glu Gly Trp
450 455 460
Ser Met Ile Arg Cys Asn Asp Ala Gly Ile Phe Leu Thr Trp Lys Glu
465 470 475 480
Gln Pro Val Val Trp Ala Ser Ala Trp Arg Pro
485 490
<210>242
<211>2194
<212>DNA
<213>稻
<400>242
aatccgaaaa gtttctgcac cgttttcacc ccctaactaa caatataggg aacgtgtgct 60
aaatataaaa tgagacctta tatatgtagc gctgataact agaactatgc aagaaaaact 120
catccaccta ctttagtggc aatcgggcta aataaaaaag agtcgctaca ctagtttcgt 180
tttccttagt aattaagtgg gaaaatgaaa tcattattgc ttagaatata cgttcacatc 240
tctgtcatga agttaaatta ttcgaggtag ccataattgt catcaaactc ttcttgaata 300
aaaaaatctt tctagctgaa ctcaatgggt aaagagagag atttttttta aaaaaataga 360
atgaagatat tctgaacgta ttggcaaaga tttaaacata taattatata attttatagt 420
ttgtgcattc gtcatatcgc acatcattaa ggacatgtct tactccatcc caatttttat 480
ttagtaatta aagacaattg acttattttt attatttatc ttttttcgat tagatgcaag 540
gtacttacgc acacactttg tgctcatgtg catgtgtgag tgcacctcct caatacacgt 600
tcaactagca acacatctct aatatcactc gcctatttaa tacatttagg tagcaatatc 660
tgaattcaag cactccacca tcaccagacc acttttaata atatctaaaa tacaaaaaat 720
aattttacag aatagcatga aaagtatgaa acgaactatt taggtttttc acatacaaaa 780
aaaaaaagaa ttttgctcgt gcgcgagcgc caatctccca tattgggcac acaggcaaca 840
acagagtggc tgcccacaga acaacccaca aaaaacgatg atctaacgga ggacagcaag 900
tccgcaacaa ccttttaaca gcaggctttg cggccaggag agaggaggag aggcaaagaa 960
aaccaagcat cctccttctc ccatctataa attcctcccc ccttttcccc tctctatata 1020
ggaggcatcc aagccaagaa gagggagagc accaaggaca cgcgactagc agaagccgag 1080
cgaccgcctt ctcgatccat atcttccggt cgagttcttg gtcgatctct tccctcctcc 1140
acctcctcct cacagggtat gtgcctccct tcggttgttc ttggatttat tgttctaggt 1200
tgtgtagtac gggcgttgat gttaggaaag gggatctgta tctgtgatga ttcctgttct 1260
tggatttggg atagaggggt tcttgatgtt gcatgttatc ggttcggttt gattagtagt 1320
atggttttca atcgtctgga gagctctatg gaaatgaaat ggtttaggga tcggaatctt 1380
gcgattttgt gagtaccttt tgtttgaggt aaaatcagag caccggtgat tttgcttggt 1440
gtaataaagt acggttgttt ggtcctcgat tctggtagtg atgcttctcg atttgacgaa 1500
gctatccttt gtttattccc tattgaacaa aaataatcca actttgaaga cggtcccgtt 1560
gatgagattg aatgattgat tcttaagcct gtccaaaatt tcgcagctgg cttgtttaga 1620
tacagtagtc cccatcacga aattcatgga aacagttata atcctcagga acaggggatt 1680
ccctgttctt ccgatttgct ttagtcccag aatttttttt cccaaatatc ttaaaaagtc 1740
actttctggt tcagttcaat gaattgattg ctacaaataa tgcttttata gcgttatcct 1800
agctgtagtt cagttaatag gtaatacccc tatagtttag tcaggagaag aacttatccg 1860
atttctgatc tccattttta attatatgaa atgaactgta gcataagcag tattcatttg 1920
gattattttt tttattagct ctcacccctt cattattctg agctgaaagt ctggcatgaa 1980
ctgtcctcaa ttttgttttc aaattcacat cgattatcta tgcattatcc tcttgtatct 2040
acctgtagaa gtttcttttt ggttattcct tgactgcttg attacagaaa gaaatttatg 2100
aagctgtaat cgggatagtt atactgcttg ttcttatgat tcatttcctt tgtgcagttc 2160
ttggtgtagc ttgccacttt caccagcaaa gttc 2194
<210>243
<211>60
<212>DNA
<213>人工序列
<220>
<223>引物:prm07341
<400>243
ggggacaagt ttgtacaaaa aagcaggctt aaacaatgga tactctcttt agactagtca 60
<210>244
<211>50
<212>DNA
<213>人工序列
<220>
<223>引物:prm07342
<400>244
ggggaccact ttgtacaaga aagctgggta aataaaaaca accctttacg 50
Claims (24)
1.用于相对于对照植物增强植物中产量相关性状的方法,其包括调节选自以下的核酸的表达:
a)在植物中,编码TCP1或TCP2多肽的核酸;
b)在植物中,编码底物蛋白-样多肽的核酸,其中所述底物蛋白-样多肽包含ENTH结构域,
c)在植物种子中,编码tRNAδ(2)-异戊烯焦磷酸转移酶(IPPT)多肽的核酸序列,所述IPPT多肽包含:(i)InterPro登录号IPR002627的tRNA异戊烯基转移酶结构域;和(ii)N端ATP/GTP结合位点基序A(P-环),和任选选择具有增强的产量相关性状的植物,和
d)在生长于亚最佳养分可利用性条件下的植物中,编码SHR多肽的核酸。
2.根据权利要求1的用于相对于对照植物增强植物中产量相关性状的方法,其包含调节编码TCP1或TCP2多肽的核酸在植物中的表达,所述TCP1多肽包含:
(i)与图1所示任一序列的TCP结构域以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的TCP结构域;和
(ii)与图1所示任一序列的结构域A以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iii)与图1所示任一序列的结构域B以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iv)与图1所示任一序列的结构域C以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域,
且所述TCP2多肽包含:
(i)与图2所示任意序列的TCP结构域以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的TCP结构域;和
(ii)与图2所示任意序列的结构域1以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iii)与图2所示任意序列的结构域2以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;和
(iv)与图2所示任意序列的结构域3以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域。
3.根据权利要求2的方法。其中所述TCP2多肽包含:
(v)与图2所示任意序列的结构域4以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域;
(vi)与图2所示任意序列的结构域5以增加的优选顺序具有至少50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或更高序列同一性的结构域。
4.根据权利要求2或3的方法,其中所述TCP1多肽在构建诸如图1所示的TCP系统树时,倾向于与包含SEQ ID NO:2所示的多肽序列的TCP进化支聚簇,而不与任何其他TCP进化支聚簇。
5.根据权利要求2或3的方法,其中所述TCP2多肽在构建诸如图2所示的TCP系统树时,倾向于与包含SEQ ID NO:4所示的多肽序列的TCP进化支聚簇,而不与任何其他TCP进化支聚簇。
6.根据任一前述权利要求的方法,其中所述核酸序列编码SEQ IDNO:2或4的直向同源物或旁系同源物。
7.根据任一前述权利要求的方法,其中所述调节的表达是编码TCP1或TCP2多肽的核酸增加的表达。
8.根据权利要求7的方法,其中所述增加的表达通过T-DNA活化标签技术、TILLING或同源重组中的一种或多种实现。
9.根据权利要求7的方法,其中所述增加的表达通过在植物中引入和表达编码TCP1或TCP2多肽的核酸序列实现。
10.根据任一前述权利要求的方法,其中所述增强的产量相关性状包括相对于对照植物的提高的种子重量。
11.根据权利要求9或10的方法,其中所述核酸序列与组成型启动子有效连接,优选与HMGP(高迁移率族蛋白)启动子或至GOS2启动子有效连。
12.根据权利要求8至10中任一项的方法,其中所述编码TCP1或TCP2多肽的核酸序列优选是植物来源的,进一步优选来自双子叶植物,更优选来自苜蓿科,最优选来自苜蓿或蒺藜苜蓿。
13.根据权利要求1至12中任一项的方法可获得的植物、其包括种子的部分,其中所述的植物或其部分包含编码TCP1或TCP2多肽的核酸转基因。
14.构建体,其包含:
(i)编码TCP1或TCP2多肽的核酸序列;
(ii)能够驱动(i)的核酸序列表达的一个或多个调控序列;和任选地
(iii)转录终止序列。
15.根据权利要求14的构建体,其中所述一个或多个调控序列至少是组成型启动子,优选是HMGP或GOS2启动子。
16.根据权利要求14或15的构建体在制备植物中的用途,所述植物相对于对照植物具有提高的产量,尤其具有提高的种子产量。
17.用根据权利要求14或15的构建体转化的植物、植物部分或植物细胞。
18.用于制备相对于对照植物具有提高的种子产量的转基因植物的方法,所述方法包括:
(i)在植物或植物细胞中引入并表达编码TCP1或TCP2多肽的核酸序列;和
(ii)在促进植物生长和发育的条件下培育所述植物细胞。
19.转基因植物,其相对于对照植物具有因编码TCP1或TCP2多肽的核酸的表达增加引起的提高的产量,尤其提高的种子产量,或者从所述转基因植物衍生的转基因植物细胞。
20.根据权利要求18的转基因植物,其中所述提高的种子产量是以下一项或多项:(i)提高的种子重量;(ii)提高的收获指数;(iii)提高的千粒重;(iv)提高的每穗花数;(v)提高的饱满率;或(vii)提高的饱满种子数。
21.根据权利要求13、17、19或20中任一项的转基因植物或从所述转基因植物衍生的转基因植物细胞,其中所述的植物是作物植物或单子叶植物或谷物植物,如稻、玉米、小麦、大麦、粟、黑麦、高粱和燕麦。
22.根据权利要求21的植物的可收获部分,其中所述的可收获部分优选是种子。
23.产物,其衍生自根据权利要求21的植物和/或根据权利要求22的植物的可收获部分。
24.编码TCP1或TCP2多肽的核酸在提高植物的产量、尤其是种子产量中的用途。
Applications Claiming Priority (17)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP07123820.8 | 2007-12-20 | ||
EP07123820 | 2007-12-20 | ||
EP07124036.0 | 2007-12-21 | ||
EP07124036 | 2007-12-21 | ||
EP07124011 | 2007-12-21 | ||
EP07124011.3 | 2007-12-21 | ||
EP07025090 | 2007-12-24 | ||
EP07025090.7 | 2007-12-24 | ||
US2715508P | 2008-02-08 | 2008-02-08 | |
US2710508P | 2008-02-08 | 2008-02-08 | |
US61/027,105 | 2008-02-08 | ||
US61/027,155 | 2008-02-08 | ||
US2751308P | 2008-02-11 | 2008-02-11 | |
US2749908P | 2008-02-11 | 2008-02-11 | |
US61/027,513 | 2008-02-11 | ||
US61/027,499 | 2008-02-11 | ||
PCT/EP2008/068129 WO2009080802A2 (en) | 2007-12-20 | 2008-12-22 | Plants having enhanced yield-related traits and a method for making the same |
Publications (1)
Publication Number | Publication Date |
---|---|
CN101969759A true CN101969759A (zh) | 2011-02-09 |
Family
ID=40801617
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2008801202018A Pending CN101969759A (zh) | 2007-12-20 | 2008-12-22 | 具有增强的产量相关性状的植物及其制备方法 |
Country Status (8)
Country | Link |
---|---|
US (2) | US8575421B2 (zh) |
EP (1) | EP2240009A2 (zh) |
CN (1) | CN101969759A (zh) |
AR (1) | AR072133A1 (zh) |
AU (1) | AU2008339968A1 (zh) |
CA (1) | CA2708506A1 (zh) |
DE (1) | DE112008003316T5 (zh) |
WO (1) | WO2009080802A2 (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109136147A (zh) * | 2018-09-21 | 2019-01-04 | 南京农业大学 | 一种多重重金属耐性产吲哚乙酸的菌株及其应用 |
CN111087459A (zh) * | 2020-01-14 | 2020-05-01 | 上海交通大学 | 一种青蒿TCP类转录因子AaTCP15及其应用 |
CN114181290A (zh) * | 2020-09-15 | 2022-03-15 | 中国科学院遗传与发育生物学研究所 | 一个调控植物氮素利用效率和产量的蛋白质及其应用 |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2008339968A1 (en) | 2007-12-20 | 2009-07-02 | Basf Plant Science Gmbh | Plants having enhanced yield-related traits and a method for making the same |
BR112012018108A2 (pt) | 2010-01-22 | 2015-10-20 | Bayer Ip Gmbh | combinações acaricidas e/ou inseticidas de ingredientes ativos |
CN103298943A (zh) * | 2010-11-10 | 2013-09-11 | 巴斯夫植物科学有限公司 | 产量相关性状增强的植物及其制备方法 |
CN103533827A (zh) * | 2011-05-11 | 2014-01-22 | 巴斯夫植物科学有限公司 | 具有增强的产量相关性状的植物和用于产生该植物的方法 |
US9265252B2 (en) | 2011-08-10 | 2016-02-23 | Bayer Intellectual Property Gmbh | Active compound combinations comprising specific tetramic acid derivatives |
Family Cites Families (75)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4962028A (en) | 1986-07-09 | 1990-10-09 | Dna Plant Technology Corporation | Plant promotors |
US5004863B2 (en) | 1986-12-03 | 2000-10-17 | Agracetus | Genetic engineering of cotton plants and lines |
US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
US5116742A (en) | 1986-12-03 | 1992-05-26 | University Patents, Inc. | RNA ribozyme restriction endoribonucleases and methods |
JP2996995B2 (ja) | 1988-06-01 | 2000-01-11 | ザ テキサス エイ アンド エム ユニヴァーシティ システム | 茎頂による植物の形質転換方法 |
WO1993022443A1 (en) | 1992-04-24 | 1993-11-11 | Sri International | In vivo homologous sequence targeting in eukaryotic cells |
DE69333937T2 (de) | 1992-06-29 | 2006-08-31 | Gene Shears Pty. Ltd. | Nukleinsäuren und sie verwendende verfahren zur bekämpfung viraler pathogene |
US5401836A (en) | 1992-07-16 | 1995-03-28 | Pioneer Hi-Bre International, Inc. | Brassica regulatory sequence for root-specific or root-abundant gene expression |
JPH08503853A (ja) | 1992-11-30 | 1996-04-30 | チューア,ナム−ハイ | 植物における組織−及び発生−特異的な発現を付与する発現モチーフ |
JPH09505461A (ja) | 1993-07-22 | 1997-06-03 | ジーン シェアーズ プロプライアタリー リミティド | Dnaウィルスリボザイム |
WO1995014098A1 (en) | 1993-11-19 | 1995-05-26 | Biotechnology Research And Development Corporation | Chimeric regulatory regions and gene cassettes for expression of genes in plants |
PT733059E (pt) | 1993-12-09 | 2001-03-30 | Univ Jefferson | Compostos e metodos para mutacoes dirigidas ao local em celulas eucarioticas |
US6395547B1 (en) | 1994-02-17 | 2002-05-28 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
AU718082B2 (en) | 1995-10-06 | 2000-04-06 | Plant Genetic Systems N.V. | Seed shattering |
US7390937B2 (en) | 1996-02-14 | 2008-06-24 | The Governors Of The University Of Alberta | Plants with enhanced levels of nitrogen utilization proteins in their root epidermis and uses thereof |
GB9607517D0 (en) | 1996-04-11 | 1996-06-12 | Gene Shears Pty Ltd | The use of DNA Sequences |
GB9703146D0 (en) | 1997-02-14 | 1997-04-02 | Innes John Centre Innov Ltd | Methods and means for gene silencing in transgenic plants |
GB9710475D0 (en) | 1997-05-21 | 1997-07-16 | Zeneca Ltd | Gene silencing |
GB9720148D0 (en) | 1997-09-22 | 1997-11-26 | Innes John Centre Innov Ltd | Gene silencing materials and methods |
JP5015373B2 (ja) | 1998-04-08 | 2012-08-29 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション | 改良表現型を得るための方法及び手段 |
US6764851B2 (en) | 1998-06-26 | 2004-07-20 | Iowa State University Research Foundation, Inc. | Materials and methods for the alteration of enzyme and acetyl CoA levels in plants |
US6555732B1 (en) | 1998-09-14 | 2003-04-29 | Pioneer Hi-Bred International, Inc. | Rac-like genes and methods of use |
US20030121070A1 (en) * | 2000-08-22 | 2003-06-26 | Luc Adam | Genes for modifying plant traits IV |
US20040031072A1 (en) | 1999-05-06 | 2004-02-12 | La Rosa Thomas J. | Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement |
KR100455617B1 (ko) | 1999-07-22 | 2004-11-06 | 독립행정법인농업생물자원연구소 | 단자엽 식물의 초신속 형질전환 방법 |
CA2379498C (en) | 1999-08-26 | 2012-12-18 | Basf Plant Science Gmbh | Plant gene expression, controlled by constitutive plant v-atpase promoters |
US6927320B1 (en) | 2000-05-24 | 2005-08-09 | New York University | Short-root gene, promoter, and uses thereof |
US20050108791A1 (en) | 2001-12-04 | 2005-05-19 | Edgerton Michael D. | Transgenic plants with improved phenotypes |
US7235710B2 (en) | 2003-01-21 | 2007-06-26 | Cropdesign N.V. | Regulatory sequence |
EP2180055B1 (en) | 2003-02-04 | 2011-10-19 | CropDesign N.V. | Rice promoters |
IL156719A0 (en) | 2003-06-30 | 2004-01-04 | Axiomic Technologies Inc | A multi-stage open ion system in various topologies |
US7705201B2 (en) | 2004-06-23 | 2010-04-27 | Monsanto Technology Llc | Transgenic plants expressing cytokinin biosynthetic genes and methods of use therefor |
EP2489726A3 (en) | 2005-01-12 | 2012-11-28 | Monsanto Technology LLC | Genes and uses for plant improvement |
EP2327770A1 (en) | 2005-11-07 | 2011-06-01 | CropDesign N.V. | Use of Group I shaggy-like kinases for increasing salt-stress resistance and yield of plants |
US8853492B2 (en) * | 2005-11-07 | 2014-10-07 | Cropdesign N.V. | Plants having improved growth characteristics and a method for making the same |
AR059650A1 (es) * | 2006-02-28 | 2008-04-16 | Cropdesign Nv | Plantas que presentan aumento en el rendimiento y metodo para lograrlo |
US8642838B2 (en) | 2006-03-31 | 2014-02-04 | Basf Plant Science Gmbh | Plants having enhanced yield-related traits and a method for making the same |
US20090241218A1 (en) * | 2006-05-30 | 2009-09-24 | Cropdesign N.V. | Plants having enhanced yield-related traits and a method for making the same |
EP2054516B1 (en) * | 2006-08-02 | 2014-01-08 | CropDesign N.V. | Plants having improved characteristics and a method for making the same |
CA2664729A1 (en) * | 2006-11-16 | 2008-05-22 | Basf Plant Science Gmbh | Plants having enhanced yield-related traits and a method for making the same using consensus sequences from the yabby protein family |
US8350119B2 (en) * | 2006-11-24 | 2013-01-08 | Cropdesign N.V. | Transgenic plants comprising as transgene A class I TCP or Clavata 1 (CLV1) or CAH3 polypeptide having increased seed yield and a method for making the same |
WO2008071767A1 (en) * | 2006-12-15 | 2008-06-19 | Cropdesign N.V. | Plants having enhanced seed yield-related traits and a method for making the same |
CN101583720A (zh) * | 2006-12-21 | 2009-11-18 | 巴斯福植物科学有限公司 | 具有增强的产量相关性状的植物和用于产生该植物的方法 |
EP2548963A1 (en) * | 2007-01-30 | 2013-01-23 | CropDesign N.V. | Plants having enhanced yield-related traits and a method for making the same |
DE112008000275T5 (de) * | 2007-01-31 | 2009-12-31 | Basf Plant Science Gmbh | Pflanzen mit verbesserten Ertragsmerkmalen und/oder erhöhter Resistenz gegen abiotischen Stress und Verfahren zu ihrer Herstellung |
EP2069509B1 (en) * | 2007-05-03 | 2013-09-18 | BASF Plant Science GmbH | Plants having enhanced yield-related traits and a method for making the same |
WO2009003977A2 (en) * | 2007-06-29 | 2009-01-08 | Basf Plant Science Gmbh | Plants having enhanced yield-related traits and a method for making the same |
ES2440265T3 (es) * | 2007-07-20 | 2014-01-28 | Basf Plant Science Gmbh | Plantas que tienen un aumento de características relacionadas con el rendimiento y un método para elaboración de las mismas |
AR070640A1 (es) * | 2007-07-20 | 2010-04-28 | Cropdesign Nv | Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para obtenerlas |
AR067748A1 (es) * | 2007-07-31 | 2009-10-21 | Basf Plant Science Gmbh | Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para obtenerlas |
WO2009034188A1 (en) * | 2007-09-14 | 2009-03-19 | Basf Plant Science Gmbh | Plants having increased yield-related traits and a method for making the same |
MX2010002463A (es) * | 2007-09-21 | 2010-03-29 | Basf Plant Science Gmbh | Plantas con rasgos relacionados con un rendimiento aumentado y un metodo para producirlas. |
CA2703827A1 (en) * | 2007-10-29 | 2009-05-07 | Basf Plant Science Gmbh | Plants having enhanced yield-related traits and a method for making the same |
MX2010004699A (es) * | 2007-11-22 | 2010-05-14 | Cropdesign Nv | Plantas que tienen rasgos incrementados relacionados con el rendimiento y un metodo para producirlas. |
AR071545A1 (es) * | 2007-11-26 | 2010-06-30 | Basf Plant Science Gmbh | Plantas con rasgos aumentados relacionados con el rendimiento y un metodo para desarrollarlas |
EP2222857A2 (en) * | 2007-12-19 | 2010-09-01 | BASF Plant Science GmbH | Plants with increased yield and/or increased tolerance to environmental stress (iy-bm) |
AU2008339968A1 (en) | 2007-12-20 | 2009-07-02 | Basf Plant Science Gmbh | Plants having enhanced yield-related traits and a method for making the same |
CN101965405B (zh) * | 2008-01-25 | 2014-03-05 | 巴斯夫植物科学有限公司 | 具有增强的产量相关性状的植物和用于制备该植物的方法 |
AR070693A1 (es) * | 2008-01-31 | 2010-04-28 | Basf Plant Science Gmbh | Plantas que tienen rasgos aumentados relacionados con el rendimiento y un metodo para obtenerlas a partir de la expresion del polipeptido de nitrato 2 (nrt2) |
BRPI0910576A2 (pt) * | 2008-04-16 | 2015-08-04 | Basf Plant Science Gmbh | Métodos para intensificar e/ou aumentar características relacionadas com rendimento em plantas, e para a produção de uma planta transgênica apresentando rendimento aumentado, construção, usos de uma construção e de um ácido nucleico que codifica um polipeptídeo de ornitina descarboxilase, planta, parte de planta ou célula de planta, planta transgênica, partes colhíveis de uma planta, e, produtos. |
EP2274431A1 (en) * | 2008-05-05 | 2011-01-19 | BASF Plant Science GmbH | Plants having enhanced yield-related traits and a method for making the same |
CN103834683A (zh) * | 2008-06-20 | 2014-06-04 | 巴斯夫植物科学有限公司 | 具有增强的产量相关性状的植物和产生所述植物的方法 |
MX2010013622A (es) * | 2008-06-26 | 2011-02-15 | Basf Plant Science Gmbh | Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para obtenerlas. |
AU2009265701A1 (en) * | 2008-07-04 | 2010-01-07 | Basf Plant Science Gmbh | Plants having enhanced yield-related traits and a method for making the same by overexpressing a polynucleotide encoding a TFL1-like protein |
MX2011000483A (es) * | 2008-07-17 | 2011-02-24 | Basf Plant Science Gmbh | Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para producir las mismas. |
CN102144033A (zh) * | 2008-07-31 | 2011-08-03 | 巴斯夫植物科学有限公司 | 具有修饰的生长特征的植物及其制备方法 |
AU2009284263B2 (en) * | 2008-08-20 | 2015-04-09 | Basf Plant Science Gmbh | Plants having enhanced yield-related traits and a method for making the same |
BRPI0917356A2 (pt) * | 2008-08-29 | 2019-09-24 | Basf Plant Science Co Gmbh | método para realçar características relacionadas com o rendimento em uma planta em relação às plantas de controle, construção, uso de uma construção, planta, parte de planta ou célula de planta, método para a produção de uma planta transgênica, partes colhíveis de uma planta, produtos, e, uso de qualquer dois ou três ácidos nucleicos |
CN102224247B (zh) * | 2008-09-24 | 2016-09-28 | 巴斯夫植物科学有限公司 | 具有改良的产量相关性状的植物和用于制备该植物的方法 |
AU2009306575B2 (en) * | 2008-10-23 | 2016-04-14 | Basf Plant Science Gmbh | Plants with increased yield (NUE) |
MX2011004785A (es) * | 2008-11-12 | 2011-06-16 | Basf Plant Science Gmbh | Plantas que tienen mejor tolerancia al estres abiotico y/o mejores rasgos relacionados con el rendimiento y un metodo para producirlas. |
BRPI0922553A2 (pt) * | 2008-12-03 | 2013-05-07 | Basf Plant Science Gmbh | mÉtodo para aumentar a tolerÂncia a estresse abiàtico e/ou para aumentar uma caracterÍstica relacionada ao rendimento em uma planta, construto, planta, cÉlula de planta ou parte da mesma, incluindo sementes, partes cultivÁveis da dita planta, produtos derivados de dita planta e/ou de ditas partes cultivÁveis, mÉtodo para a produÇço de plantas transgÊnicas, uso de um construto ou de um Ácido nucleico, molÉcula de Ácido nucleico isolada, e, polipeptÍdeo isolado |
AU2009328306A1 (en) * | 2008-12-17 | 2011-07-14 | Basf Plant Science Gmbh | Plants having enhanced yield-related traits and/or abiotic stress tolerance and a method for making the same |
US8778660B2 (en) * | 2010-04-24 | 2014-07-15 | Newleaf Symbiotics, Inc. | Method for increasing algae growth and the use thereof in production of algae-derived biofuels and other chemical |
-
2008
- 2008-12-22 AU AU2008339968A patent/AU2008339968A1/en not_active Abandoned
- 2008-12-22 US US12/808,208 patent/US8575421B2/en not_active Expired - Fee Related
- 2008-12-22 EP EP08865904A patent/EP2240009A2/en not_active Withdrawn
- 2008-12-22 DE DE112008003316T patent/DE112008003316T5/de not_active Withdrawn
- 2008-12-22 CN CN2008801202018A patent/CN101969759A/zh active Pending
- 2008-12-22 WO PCT/EP2008/068129 patent/WO2009080802A2/en active Application Filing
- 2008-12-22 CA CA2708506A patent/CA2708506A1/en not_active Abandoned
- 2008-12-23 AR ARP080105695A patent/AR072133A1/es not_active Application Discontinuation
-
2013
- 2013-09-24 US US14/035,211 patent/US20140096283A1/en not_active Abandoned
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109136147A (zh) * | 2018-09-21 | 2019-01-04 | 南京农业大学 | 一种多重重金属耐性产吲哚乙酸的菌株及其应用 |
CN111087459A (zh) * | 2020-01-14 | 2020-05-01 | 上海交通大学 | 一种青蒿TCP类转录因子AaTCP15及其应用 |
CN111087459B (zh) * | 2020-01-14 | 2023-04-07 | 上海交通大学 | 一种青蒿TCP类转录因子AaTCP15在调控青蒿素表达量中的应用 |
CN114181290A (zh) * | 2020-09-15 | 2022-03-15 | 中国科学院遗传与发育生物学研究所 | 一个调控植物氮素利用效率和产量的蛋白质及其应用 |
WO2022057780A1 (zh) * | 2020-09-15 | 2022-03-24 | 中国科学院遗传与发育生物学研究所 | 一个调控植物氮素利用效率和产量的蛋白质及其应用 |
CN114181290B (zh) * | 2020-09-15 | 2023-10-27 | 中国科学院遗传与发育生物学研究所 | 一个调控植物氮素利用效率和产量的蛋白质及其应用 |
Also Published As
Publication number | Publication date |
---|---|
CA2708506A1 (en) | 2009-07-02 |
AR072133A1 (es) | 2010-08-11 |
DE112008003316T5 (de) | 2011-05-05 |
WO2009080802A3 (en) | 2010-09-10 |
US8575421B2 (en) | 2013-11-05 |
AU2008339968A1 (en) | 2009-07-02 |
US20100269219A1 (en) | 2010-10-21 |
US20140096283A1 (en) | 2014-04-03 |
WO2009080802A2 (en) | 2009-07-02 |
EP2240009A2 (en) | 2010-10-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101495640B (zh) | 具有增强的产量相关性状的伸展蛋白受体样激酶受调节表达的植物和用于产生该植物的方法 | |
KR101662483B1 (ko) | 향상된 수확량 관련 형질을 갖는 식물 및 이의 제조 방법 | |
CN101842489B (zh) | 具有增强的产量相关性状的植物和用于制备该植物的方法 | |
EP2004829B1 (en) | Plants having enhanced yield-related traits and a method for making the same | |
AU2009284263B2 (en) | Plants having enhanced yield-related traits and a method for making the same | |
KR101754083B1 (ko) | 향상된 수확량 관련 형질을 갖는 식물 및 이의 제조 방법 | |
KR101255415B1 (ko) | 향상된 수확량 관련 형질을 갖는 식물 및 이의 제조 방법 | |
KR101255413B1 (ko) | 향상된 수확량 관련 형질을 갖는 식물 및 이의 제조 방법 | |
CN101952441B (zh) | 具有增强的产量相关性状的植物及其制备方法 | |
EP2508610A1 (en) | Plants having enhanced yield-related traits and a method for making the same | |
CN101883783A (zh) | 具有增强的产量相关性状的植物及其制备方法 | |
CN101583720A (zh) | 具有增强的产量相关性状的植物和用于产生该植物的方法 | |
EP2711424A2 (en) | Plants having enhanced yield-related traits and a method for making the same | |
KR20120126061A (ko) | 향상된 수확량 관련 형질을 갖는 식물 및 이의 제조 방법 | |
CN101855355A (zh) | 具有提高的产量相关性状的植物和用于制备该植物的方法 | |
BRPI0718977A2 (pt) | Método para aumentar rendimento de sementes em plantas em relação às plantas de controle, construção, uso da mesma, planta, parte de planta ou célula de planta, método para a produção de uma planta transgênica tendo redimento aumentado de sementes em relação às plantas de controle, planta transgênica, partes colhíveis de uma planta, produtos, e, uso de um ácido nucleico | |
MX2010010735A (es) | Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para obtenerlas. | |
CN101868544A (zh) | 具有提高的产量相关性状的植物和用于制备该植物的方法 | |
KR101429468B1 (ko) | 향상된 수확량 관련 형질을 갖는 식물 및 이의 제조 방법 | |
CN101605902A (zh) | 具有增强的产量相关性状和/或提高的非生物胁迫抗性的植物和制备该植物的方法 | |
CN101563461A (zh) | 具有改良特征的植物及其制备方法 | |
CN101969759A (zh) | 具有增强的产量相关性状的植物及其制备方法 | |
CN101778942A (zh) | 产率相关性状增强的植物及制备其的方法 | |
CN101595222B (zh) | 具有改良的种子产量相关性状的植物及其制备方法 | |
CN101668859A (zh) | 具有增强的产量相关性状的植物及其制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C12 | Rejection of a patent application after its publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20110209 |