CN101946665B - Method for cuttage and culture of seedling of Melia azedarach Linn - Google Patents
Method for cuttage and culture of seedling of Melia azedarach Linn Download PDFInfo
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- CN101946665B CN101946665B CN201010245963XA CN201010245963A CN101946665B CN 101946665 B CN101946665 B CN 101946665B CN 201010245963X A CN201010245963X A CN 201010245963XA CN 201010245963 A CN201010245963 A CN 201010245963A CN 101946665 B CN101946665 B CN 101946665B
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Abstract
The invention discloses a method for cuttage and culture of seedling of Melia azedarach Linn, comprising the following steps: (1) preparing a cuttage matrix; (2) preparing rooting agent; (3) selecting materials and cutting; and (4) managing and transplanting after cottage. The method for cuttage and culture of seedling of the invention can keep the excellent performance of the stock plant of the Melia azedarach Linn, and has the advantages of high reproduction coefficient, strong and tidy plant growth, simple and convenient operation and the like. Compared with the root buried seedling culture method, the invention improves the nursery stock breeding amount by above 80%, the operation program of the cuttage technology is easy to popularize and apply, the cost is one tenth of that of propagation by tissue culture, and the invention has the important practical and popularizing meaning on the Melia azedarach Linn nursery stock breeding.
Description
Technical field
The present invention relates to a plant species asexual reproduction method, specifically relate to a kind of China tree cuttage and seedling culture method.
Background technology
The breeding of China tree is main with planting seed; Soil requires high, need draining good, irrigate deep easily fertile sandy loam, at spring or planted in fall; Kind of the rate that goes out of the China tree of this kind method breeding is 25%～40%; Germination rate is 60%～80%, and every mu of seeding quantity 15～20kg needs 40～50 talentes to begin to germinate approximately; In addition, because margosa root sprout tiller strength is strong, can also carry out breeding and seedling with chinaberry root; In recent years, the scholar is arranged, use the suitable culture base, through control illumination, damp condition tissue culture propagating China tree with China tree grow thickly bud and effective tender stem.In the above-mentioned propagation method, the seminal propagation of China tree, it is all lower that it goes out kind of a rate, germination rate, and China tree kind shell is harder, and the unearthed time of seed sprouting is longer, influence growing of China tree, and seminal propagation can not keep breeding original excellent characteristic; Though China tree is buried the hereditary quality that root propagation can keep maternal plant, root quantity is limited, gets the root difficulty, has limited the production of China tree large-scale breeding; And the in vitro tissue cultivation of China tree need be under aseptic condition, screening suitable culture base, and in adapt circumstance, carry out continuous culture and grow up to plant, the method culture technique is high, complicated operation is strict, and production cost is high.
Summary of the invention
In order to overcome the deficiency of prior art, the object of the present invention is to provide a kind of simple to operate, cost is low, reproductive efficiency is high China tree cuttage and seedling culture method.
The present invention realizes through following technical scheme:
A kind of China tree cuttage and seedling culture method is characterized in that, may further comprise the steps:
(1) preparation cutting medium: perlite and peat are mixed and made into cutting medium by 2: 1～1: 1 volume ratio, and utilization is diluted 800～1000 times broad-spectrum germicide spray solution cutting medium it is carried out disinfection;
(2) preparation root-growing agent: indolebutyric acid 800～1000mg is dissolved in 95% the ethanol of 10～20ml, adds ammonium molybdate 5～10mg, lanthanum nitrate 25～50mg and sucrose 10～30mg then, add water to 1kg at last, mix the back and form root-growing agent;
(3) selection and cuttage: in first arrival in June cloudy day or early morning by the end of July, temperature is 20～30 ℃, and humidity is 75%～90%; Clip process in early spring is cut a branch of doing back China tree rudiment growing to 20～25cm; Its clip length is 8～10cm, and clip is smooth up and down, and last clip is apart from leaf bud 1cm ± 0.2cm; Keeping compound leaf is the slotting fringe of branch section work of 1～2 vanelets; The base portion of inserting fringe is dipped 1～2min sterilization in 0.1% liquor potassic permanganate, after in the root-growing agent that step (2) prepares, dipping rapidly then, in the cutting medium that inserting step (1) configures;
(4) insert the back management and transplant: place the full exposure environment inserting good slotting fringe in the step (3); Utilize intermittent automatic sprayer; Whenever at a distance from 5～15min spraying 1 time, each spray time is 10～20s, regulates the Time And Frequency that intermittent automatic sprayer is sprayed according to temperature and illumination condition; Keep inserting the moisture content on fringe blade face; Insert the fringe base portion after 10～15 days and begin to form callus, grow fibrous root from the callus of slotting fringe base portion or the skin zone of slotting fringe base portion in the time of 20～25 days, transplant after 60 days.
Further, the broad-spectrum germicide described in the step (1) is carbendazim or tpn solution.
The invention has the beneficial effects as follows:
1, adopt cuttage and seedling culture method of the present invention can keep the merit of China tree maternal plant, and the breeding cycle is short, seedling growth is fast, is the valid approach of China tree breeding vegetative propagation, the descendant inheritting variation problem of avoiding China tree seminal propagation to produce;
2, adopt cuttage and seedling culture method of the present invention and China tree to bury root and grow seedlings and compare, have advantages such as reproduction rate height, plant strain growth be healthy and strong, neat, easy and simple to handle;
3, described cuttage and seedling culture method of the present invention is compared with the China tree method for tissue culture; Have that cost is low, specification requirement is easy; The characteristics that are prone to apply; Overcome that China tree tissue culture one-time investment is big, cost is high, technical step is numerous and diverse, that technology is prone to biography property is poor, on producing, can not directly utilize test-tube plantlet, transplanting survival rate low, promote shortcomings such as difficulty is big;
In sum: adopt cuttage and seedling culture method of the present invention can keep the merit of China tree maternal plant; Have advantages such as reproduction coefficient height, plant strain growth be healthy and strong, neat, easy and simple to handle; Its seedling propagation quantity is buried the root seedling-cultivating method and is improved more than 80%; Easy being prone to of cuttage technique operation sequence applied, and its cost is 1/10 of a tissue culture propagating, in the production of China tree seedling propagation, has important practice and dissemination.
Embodiment
To combine specific embodiment to specify embodiment of the present invention below:
The China tree cuttage and seedling culture method is characterized in that, may further comprise the steps:
(1) preparation cutting medium: perlite and peat are mixed and made into cutting medium by 2: 1～1: 1 volume ratio; Utilize the broad-spectrum germicide spray solution cutting medium of 800～1000 times of dilutions that it is carried out disinfection; Wherein, Broad-spectrum germicide can be carbendazim, also can be tpn solution;
(2) preparation root-growing agent: indolebutyric acid 800～1000mg is dissolved in 95% the ethanol of 10～20ml, adds ammonium molybdate 5～10mg, lanthanum nitrate 25～50mg and sucrose 10～30mg then, add water to 1kg at last, mix the back and form root-growing agent;
(3) selection and cuttage: in first arrival in June cloudy day or early morning by the end of July, temperature is 20～30 ℃, and humidity is 75%～90%; Clip process in early spring is cut a branch of doing back China tree rudiment growing to 20～25cm; Its clip length is 8～10cm, and clip is smooth up and down, and last clip is apart from leaf bud 1cm ± 0.2cm; Keeping compound leaf is the slotting fringe of branch section work of 1～2 vanelets; The base portion of inserting fringe is dipped 1～2min sterilization in 0.1% liquor potassic permanganate, after in the root-growing agent that step (2) prepares, dipping rapidly then, in the cutting medium that inserting step (1) configures;
(4) insert the back management and transplant: place the full exposure environment inserting good slotting fringe in the step (3); Utilize intermittent automatic sprayer; Whenever at a distance from 5～15min spraying 1 time, each spray time is 10～20s, regulates the Time And Frequency that intermittent automatic sprayer is sprayed according to temperature and illumination condition; Keep inserting the moisture content on fringe blade face; Insert the fringe base portion after 10～15 days and begin to form callus, grow fibrous root from the callus of slotting fringe base portion or the skin zone of slotting fringe base portion in the time of 20～25 days, transplant after 60 days.
Specific embodiment is following:
Embodiment 1:
Perlite and peat are mixed and made into cutting medium by 2: 1 volume ratio, in the plastic seeding culturing case of the 50cm * 80cm that packs into * 15cm (length * wide * height), with the carbendazim solution spray disinfectant of 800 times of dilutions; Will be with indolebutyric acid (IBA) 800mg, the ammonium molybdate ((NH of the dissolve with ethanol of 10～20ml95%
4)
6Mo
7O
244H
2O) 10mg lanthanum nitrate (La (NO
3)
3) 25mg and sucrose 30mg, and then add water to 1kg, mix the back and form root-growing agent; In first arrival in June cloudy day or early morning by the end of July, temperature is 20～30 ℃, and humidity is 75%～90%; Clip process in early spring is cut a branch of doing back China tree rudiment growing to 20～25cm, and its clip length is 8～10cm, and clip is smooth up and down; Last clip is apart from leaf bud 1cm ± 0.2cm, and keeping compound leaf is the slotting fringe of branch section work of 1～2 vanelets, and the base portion of inserting fringe is dipped 1～2min sterilization in 0.1% liquor potassic permanganate; After in the root-growing agent for preparing, dipping rapidly then, insert in the plastic seeding culturing case of the cutting medium that configures, place then under the full exposure condition; Utilize intermittent automatic sprayer, whenever at a distance from 5～15min spraying 1 time, each spray time is 10～20s; Regulate the Time And Frequency of spraying according to temperature and illumination, keep inserting fringe blade face moisture content, insert the fringe base portion about 15 days and begin to form callus; Skin zone from callus or slotting fringe base portion about 25 days grows fibrous root, and rooting rate can be transplanted after reaching 80.8%, 60 day.
Embodiment 2.
Perlite and peat are mixed and made into cutting medium by 1: 1 volume ratio, in the plastic seeding culturing case of the 50cm * 80cm that packs into * 15cm (length * wide * height), with the carbendazim solution spray disinfectant of 1000 times of dilutions; Will be with indolebutyric acid (IBA) 1000mg, the ammonium molybdate ((NH of the dissolve with ethanol of 10～20ml95%
4)
6Mo
7O
244H
2O) 5mg, lanthanum nitrate (La (NO
3)
3) 50mg and sucrose 10mg, add water to 1kg then, mix the back and form root-growing agent; In first arrival in June cloudy day or early morning by the end of July, temperature is 20～30 ℃, and humidity is 75%～90%; Clip process in early spring is cut a branch of doing back China tree rudiment growing to 20～25cm, and its clip length is 8～10cm, and clip is smooth up and down; Last clip is apart from leaf bud 1cm ± 0.2cm, and keeping compound leaf is the slotting fringe of branch section work of 1～2 vanelets, and the base portion of inserting fringe is dipped 1～2min sterilization in 0.1% liquor potassic permanganate; After in the root-growing agent for preparing, dipping rapidly then, insert in the plastic seeding culturing case of the cutting medium that configures, place the full exposure environment; Utilize intermittent automatic sprayer, whenever at a distance from 5～15min spraying 1 time, each spray time is 10～20s; Regulate the Time And Frequency of spraying according to temperature and illumination, keep inserting fringe blade face moisture content, insert the fringe base portion about 10 days and begin to form callus; Skin zone from callus or slotting fringe base portion about 20 days grows fibrous root, and rooting rate can be transplanted after reaching 88.9%, 60 day.
Embodiment 3
Perlite and peat are mixed and made into cutting medium by 1.5: 1 volume ratio, in the plastic seeding culturing case of the 50cm * 80cm that packs into * 15cm (length * wide * height), with the carbendazim solution spray disinfectant of 1000 times of dilutions; Will be with indolebutyric acid (IBA) 900mg, the ammonium molybdate ((NH of the dissolve with ethanol of 10～20ml95%
4)
6Mo
7O
244H
2O) 7.5mg, lanthanum nitrate (La (NO
3)
3) 37.5mg and sucrose 20mg, add water to 1kg then, mix the back and form root-growing agent; In first arrival in June cloudy day or early morning by the end of July, temperature is 20～30 ℃, and humidity is 75%～90%; Clip process in early spring is cut a branch of doing back China tree rudiment growing to 20～25cm, and its clip length is 8～10cm, and clip is smooth up and down; Last clip is apart from leaf bud 1cm ± 0.2cm, and keeping compound leaf is the slotting fringe of branch section work of 1～2 vanelets, and the base portion of inserting fringe is dipped 1～2min sterilization in 0.1% liquor potassic permanganate; After in the root-growing agent for preparing, dipping rapidly then, insert in the plastic seeding culturing case of the cutting medium that configures, place the full exposure environment; Utilize intermittent automatic sprayer, whenever at a distance from 5～15min spraying 1 time, each spray time is 10～20s; Regulate the Time And Frequency of spraying according to temperature and illumination, keep inserting fringe blade face moisture content, insert the fringe base portion about 10 days and begin to form callus; Skin zone from callus or slotting fringe base portion about 20 days grows fibrous root, and rooting rate can be transplanted after reaching 85.6%, 60 day.
Be preferred embodiment wherein with embodiment 3.
Below disclose the present invention with preferred embodiment, so it is not in order to restriction the present invention, and all employings are equal to replacement or the technical scheme that obtained of equivalent transformation mode, all drop within protection scope of the present invention.
Claims (2)
1. a China tree cuttage and seedling culture method is characterized in that, may further comprise the steps:
(1) preparation cutting medium: perlite and peat are mixed and made into cutting medium by 2: 1～1: 1 volume ratio, and utilization is diluted 800～1000 times broad-spectrum germicide spray solution cutting medium it is carried out disinfection;
(2) preparation root-growing agent: indolebutyric acid 800～1000mg is dissolved in 95% the ethanol of 10～20ml, adds (NH then
4)
6Mo
7O
244H
2O5～10mg, La (NO
3)
325～50mg and sucrose 10～30mg add water to 1kg at last, mix the back and form root-growing agent;
(3) selection and cuttage: in first arrival in June cloudy day or early morning by the end of July, temperature is 20～30 ℃, and humidity is 75%～90%; Clip process in early spring is cut a branch of doing back China tree rudiment growing to 20～25cm; Its clip length is 8～10cm, and clip is smooth up and down, and last clip is apart from leaf bud 1cm ± 0.2cm; Keeping compound leaf is the slotting fringe of branch section work of 1～2 vanelets; The base portion of inserting fringe is dipped 1～2min sterilization in 0.1% liquor potassic permanganate, after in the root-growing agent that step (2) prepares, dipping rapidly then, in the cutting medium that inserting step (1) configures;
(4) insert the back management and transplant: place the full exposure environment inserting good slotting fringe in the step (3); Utilize intermittent automatic sprayer; Whenever at a distance from 5～15min spraying 1 time, each spray time is 10～20s, regulates the Time And Frequency that intermittent automatic sprayer is sprayed according to temperature and illumination condition; Keep inserting the moisture content on fringe blade face; Insert the fringe base portion after 10～15 days and begin to form callus, grow fibrous root from the callus of slotting fringe base portion or the skin zone of slotting fringe base portion in the time of 20～25 days, transplant after 60 days.
2. China tree cuttage and seedling culture method according to claim 1 is characterized in that, the broad-spectrum germicide described in the step (1) is carbendazim or tpn solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010245963XA CN101946665B (en) | 2010-08-05 | 2010-08-05 | Method for cuttage and culture of seedling of Melia azedarach Linn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010245963XA CN101946665B (en) | 2010-08-05 | 2010-08-05 | Method for cuttage and culture of seedling of Melia azedarach Linn |
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| CN101946665A CN101946665A (en) | 2011-01-19 |
| CN101946665B true CN101946665B (en) | 2012-04-18 |
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| CN102668853B (en) * | 2012-05-31 | 2013-10-23 | 安徽徽王食品有限公司 | Blueberry twig plant cuttage root-taking cultivation method |
| CN102919023B (en) * | 2012-11-09 | 2014-03-26 | 中国科学院华南植物园 | Method for breeding bauhinia galpinii efficiently and rapidly |
| CN103329711B (en) * | 2013-07-01 | 2015-09-16 | 江苏省林业科学研究院 | A kind of Qinggang softwood cutting propagation method |
| CN103548543B (en) * | 2013-11-08 | 2015-06-17 | 南京大渊美容保健有限公司 | Culture method for cuttage of melia azedarach seedlings |
| CN103749129B (en) * | 2014-01-15 | 2015-05-13 | 广西壮族自治区林业科学研究院 | Method for asexually and rapidly propagating homalium hainanense |
| CN104082020A (en) * | 2014-07-22 | 2014-10-08 | 江苏绿苑园林建设有限公司 | Bridal wreath stiff wood cutting seedling culture method |
| CN104067846A (en) * | 2014-07-22 | 2014-10-01 | 江苏绿苑园林建设有限公司 | Edgeworthia chrysantha lindl softwood cutting propagation method |
| CN104756820B (en) * | 2015-03-23 | 2018-04-06 | 王丽 | A kind of salt-soda soil chinaberry root inserts method of forestation |
| CN105284534A (en) * | 2015-11-03 | 2016-02-03 | 大新县生产力促进中心 | Planting method for rucea javanica |
| CN105557381A (en) * | 2016-01-26 | 2016-05-11 | 赵兰 | Cutting propagation cultivation method of Chinaberry trees |
| CN107231926A (en) * | 2017-07-07 | 2017-10-10 | 广西壮族自治区林业科学研究院 | Sinocalamus latiflorus stays section water storage cuttage breeding method |
| CN107455204A (en) * | 2017-08-08 | 2017-12-12 | 深圳市方森园林花卉有限公司 | A kind of method for improving art printing paper fan cuttage survival rate |
| CN109717077B (en) * | 2019-01-24 | 2021-11-30 | 安徽农业大学 | Culture method of melia azedarach callus |
| CN112586207A (en) * | 2020-12-10 | 2021-04-02 | 四川省农业科学院经济作物育种栽培研究所 | Efficient cutting propagation method for plants |
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| CN101473743B (en) * | 2009-01-16 | 2011-06-15 | 江苏省林业科学研究院 | Cuttage and seedling culture method without transplantation |
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