CN101939423A - 用于捕获和修饰基因组dna大片段和用合成叶绿体构建生物体的系统 - Google Patents

用于捕获和修饰基因组dna大片段和用合成叶绿体构建生物体的系统 Download PDF

Info

Publication number
CN101939423A
CN101939423A CN2008801097736A CN200880109773A CN101939423A CN 101939423 A CN101939423 A CN 101939423A CN 2008801097736 A CN2008801097736 A CN 2008801097736A CN 200880109773 A CN200880109773 A CN 200880109773A CN 101939423 A CN101939423 A CN 101939423A
Authority
CN
China
Prior art keywords
carrier
chloroplast
organism
dna
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2008801097736A
Other languages
English (en)
Chinese (zh)
Inventor
M·门德斯
B·奥奈尔
K·米克尔森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sapphire Energy Inc
Original Assignee
Sapphire Energy Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sapphire Energy Inc filed Critical Sapphire Energy Inc
Publication of CN101939423A publication Critical patent/CN101939423A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214Plastid transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2008801097736A 2007-10-05 2008-10-06 用于捕获和修饰基因组dna大片段和用合成叶绿体构建生物体的系统 Pending CN101939423A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US97802407P 2007-10-05 2007-10-05
US60/978,024 2007-10-05
PCT/US2008/011540 WO2009045550A2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts

Publications (1)

Publication Number Publication Date
CN101939423A true CN101939423A (zh) 2011-01-05

Family

ID=40042337

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2008801097736A Pending CN101939423A (zh) 2007-10-05 2008-10-06 用于捕获和修饰基因组dna大片段和用合成叶绿体构建生物体的系统

Country Status (12)

Country Link
EP (1) EP2195426B1 (enExample)
JP (1) JP5666908B2 (enExample)
KR (1) KR101389254B1 (enExample)
CN (1) CN101939423A (enExample)
AU (1) AU2008307471B2 (enExample)
CA (1) CA2698627A1 (enExample)
DK (1) DK2195426T3 (enExample)
GB (2) GB2460351B (enExample)
IL (1) IL204250A (enExample)
MX (1) MX2010003157A (enExample)
NZ (1) NZ583682A (enExample)
WO (1) WO2009045550A2 (enExample)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104277974A (zh) * 2013-07-03 2015-01-14 中国科学院植物研究所 一种分离产氢衣藻叶绿体的方法
CN104419701A (zh) * 2013-08-29 2015-03-18 天津大学 多片段dna的酵母快速组装方法
CN110669789A (zh) * 2019-12-04 2020-01-10 中国科学院烟台海岸带研究所 一种雨生红球藻叶绿体表达系统及其应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
CN115948450A (zh) * 2022-08-01 2023-04-11 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
CN116218750A (zh) * 2022-10-14 2023-06-06 天津科技大学 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2010003644A (es) 2007-10-08 2010-06-23 Synthetic Genomics Inc Ensamble de acidos nucleicos grandes.
CA2773675A1 (en) * 2009-09-15 2011-03-24 Sapphire Energy, Inc. A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
JP6226464B2 (ja) * 2013-09-30 2017-11-08 花王株式会社 チオエステラーゼ改変体を用いた脂質の製造方法
KR101946180B1 (ko) 2014-08-25 2019-02-08 지니위브 바이오사이언시스, 인코포레이티드 비-복제 형질도입 입자 및 형질도입 입자-기반 리포터 시스템
US11008602B2 (en) 2017-12-20 2021-05-18 Roche Molecular Systems, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
CN109536525B (zh) * 2019-02-20 2019-08-23 中国科学院烟台海岸带研究所 一种杜氏盐藻叶绿体同源重组空载体及其应用
CN111440733A (zh) * 2020-02-07 2020-07-24 天津大学 产松油醇的重组酿酒酵母及构建方法及应用
CN118256412B (zh) * 2024-04-01 2024-09-27 湖北大学 运动发酵单胞菌的工程菌株、制备方法及应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5866404A (en) * 1995-12-06 1999-02-02 Yale University Yeast-bacteria shuttle vector
US5976846A (en) 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
NZ502742A (en) * 1997-08-07 2004-04-30 Univ Auburn Universal chloroplast integration and expression vectors, transformed plants and products thereof
AU5217599A (en) * 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
CA2336590A1 (en) * 1999-06-07 2000-12-14 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
CN1997743B (zh) * 2004-03-03 2011-12-21 国立大学法人奈良先端科学技术大学院大学 通过叶绿体技术提高植物生产能力的方法
EP1629096A1 (en) * 2004-05-10 2006-03-01 BASF Plant Science GmbH Methods for assembling multiple expression constructs
DK2162537T3 (da) * 2007-06-01 2012-04-23 Sapphire Energy Inc Anvendelse af genetisk modificerede organismer til at frembringe biomasse-nedbrydende enzymer.
WO2009103065A2 (en) * 2008-02-15 2009-08-20 Ramesha Chakkodabylu S Compositions and methods for production of biofuels

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104277974A (zh) * 2013-07-03 2015-01-14 中国科学院植物研究所 一种分离产氢衣藻叶绿体的方法
CN104277974B (zh) * 2013-07-03 2017-09-15 中国科学院植物研究所 一种分离产氢衣藻叶绿体的方法
CN104419701A (zh) * 2013-08-29 2015-03-18 天津大学 多片段dna的酵母快速组装方法
CN110669789A (zh) * 2019-12-04 2020-01-10 中国科学院烟台海岸带研究所 一种雨生红球藻叶绿体表达系统及其应用
CN110669789B (zh) * 2019-12-04 2020-04-14 中国科学院烟台海岸带研究所 一种雨生红球藻叶绿体表达系统及其应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
CN115948450A (zh) * 2022-08-01 2023-04-11 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
WO2024026924A1 (zh) * 2022-08-01 2024-02-08 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
WO2024026923A1 (zh) * 2022-08-01 2024-02-08 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
CN115948450B (zh) * 2022-08-01 2024-02-13 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
CN116218750A (zh) * 2022-10-14 2023-06-06 天津科技大学 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用
CN116218750B (zh) * 2022-10-14 2024-11-26 天津科技大学 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用

Also Published As

Publication number Publication date
DK2195426T3 (da) 2013-09-08
WO2009045550A3 (en) 2009-07-30
EP2195426A2 (en) 2010-06-16
KR101389254B1 (ko) 2014-04-24
GB2453648C (en) 2012-12-05
JP2010539979A (ja) 2010-12-24
CA2698627A1 (en) 2009-04-09
GB2460351A (en) 2009-12-02
GB2453648B (en) 2011-01-05
WO2009045550A2 (en) 2009-04-09
MX2010003157A (es) 2010-05-21
NZ583682A (en) 2012-07-27
EP2195426A4 (en) 2010-12-01
AU2008307471A1 (en) 2009-04-09
IL204250A (en) 2012-12-31
GB0913302D0 (en) 2009-09-02
GB2460351B (en) 2011-03-30
EP2195426B1 (en) 2013-06-12
JP5666908B2 (ja) 2015-02-12
GB2453648C2 (en) 2012-12-19
GB0818260D0 (en) 2008-11-12
GB2453648A (en) 2009-04-15
GB2453648A8 (en) 2010-11-17
AU2008307471B2 (en) 2013-05-02
KR20100085930A (ko) 2010-07-29

Similar Documents

Publication Publication Date Title
CN101939423A (zh) 用于捕获和修饰基因组dna大片段和用合成叶绿体构建生物体的系统
KR101794298B1 (ko) 폴리뉴클레오타이드의 집합체 조성물 및 이에 대한 방법
CN101939434B (zh) 用于在大豆中提高种子贮藏油脂的生成和改变脂肪酸谱的来自解脂耶氏酵母的dgat基因
KR102147005B1 (ko) Fad2 성능 유전자좌 및 표적화 파단을 유도할 수 있는 상응하는 표적 부위 특이적 결합 단백질
CN101889088B (zh) 从植物基因组中切除核酸序列的方法
KR102274445B1 (ko) 게놈 삽입을 위한 방법
AU2024205703A1 (en) Wheat stem rust resistance genes and methods of use
CN101827938A (zh) 涉及rt1基因、相关的构建体和方法的具有改变的根构造的植物
CN113621642A (zh) 一种用于农作物杂交育种制种的遗传智能化育制种系统及其应用
CN101815432A (zh) 涉及编码核苷二磷酸激酶(ndk)多肽及其同源物的基因的用于修改植物根构造的方法
CN109982560A (zh) 同时基因编辑和单倍体诱导
KR20100037031A (ko) 유전자 녹아웃 중온성 및 호열성 생물체, 및 이의 사용 방법
KR20130032897A (ko) 알코올 발효 시의 알코올 에스테르의 생성 및 원위치에서의 생성물 제거
CA2730126C (en) Plant regulatory sequence
IL236992A (en) Genetically modified cyanobacteria that produce ethanol
KR20140099224A (ko) 케토-아이소발레레이트 데카르복실라제 효소 및 이의 이용 방법
CN108138121A (zh) 用微生物高水平生产长链二羧酸
CN115997023A (zh) 与大豆中疾病抗性相关联的新颖的抗性基因
CN101842488B (zh) 使用转多基因改变α-和β-生育三烯酚含量的组成和方法
KR20180137558A (ko) 유전자내 식물 형질전환을 위한 구조체 및 벡터
CN114085858B (zh) L-丝氨酸生产菌及其构建方法
CN101883843A (zh) 破坏过氧化物酶体生物合成因子蛋白(pex)以改变含油真核生物中多不饱和脂肪酸和总脂质含量
CN101918560B (zh) 在氮限制条件下具有改变的农学特性的植物以及涉及编码lnt2多肽及其同源物的基因的相关构建体和方法
CN101868545B (zh) 具有改变的根构造的植物、涉及编码富含亮氨酸重复序列激酶(llrk)多肽及其同源物的基因的相关构建体和方法
GB2475435A (en) Producing a product using shuttle vectors containing essential chloroplast genes

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20110105