MX2010003157A - Sistema para capturar y modificar piezas grandes de adn genomico y construir organismos con cloroplastos sinteticos. - Google Patents

Sistema para capturar y modificar piezas grandes de adn genomico y construir organismos con cloroplastos sinteticos.

Info

Publication number
MX2010003157A
MX2010003157A MX2010003157A MX2010003157A MX2010003157A MX 2010003157 A MX2010003157 A MX 2010003157A MX 2010003157 A MX2010003157 A MX 2010003157A MX 2010003157 A MX2010003157 A MX 2010003157A MX 2010003157 A MX2010003157 A MX 2010003157A
Authority
MX
Mexico
Prior art keywords
genomes
capturing
genomic dna
large pieces
minimal
Prior art date
Application number
MX2010003157A
Other languages
English (en)
Inventor
Bryan O'neill
Michael Mendez
Kari Mikkelson
Original Assignee
Sapphire Energy Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sapphire Energy Inc filed Critical Sapphire Energy Inc
Publication of MX2010003157A publication Critical patent/MX2010003157A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Nutrition Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere al análisis funcional de genes, frecuentemente que requiere la manipulación de regiones genómicas grandes. Se describe un vector de lanzadera de levadura-bacteria que se puede usar para clonar grandes regiones de ADN por recombinación homóloga. También se describe un método para aislar genomas completos, que incluye genomas de cloroplastos o porciones grandes del mismo, y manipulación del mismo. También descritos están métodos para determinar los genomas mínimos requerimientos de trayectoria mínima y genomas de organelo mínimos.
MX2010003157A 2007-10-05 2008-10-06 Sistema para capturar y modificar piezas grandes de adn genomico y construir organismos con cloroplastos sinteticos. MX2010003157A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97802407P 2007-10-05 2007-10-05
PCT/US2008/011540 WO2009045550A2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts

Publications (1)

Publication Number Publication Date
MX2010003157A true MX2010003157A (es) 2010-05-21

Family

ID=40042337

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010003157A MX2010003157A (es) 2007-10-05 2008-10-06 Sistema para capturar y modificar piezas grandes de adn genomico y construir organismos con cloroplastos sinteticos.

Country Status (12)

Country Link
EP (1) EP2195426B1 (es)
JP (1) JP5666908B2 (es)
KR (1) KR101389254B1 (es)
CN (1) CN101939423A (es)
AU (1) AU2008307471B2 (es)
CA (1) CA2698627A1 (es)
DK (1) DK2195426T3 (es)
GB (2) GB2453648C2 (es)
IL (1) IL204250A (es)
MX (1) MX2010003157A (es)
NZ (1) NZ583682A (es)
WO (1) WO2009045550A2 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5618413B2 (ja) 2007-10-08 2014-11-05 シンセティック ゲノミクス、インク． 大型核酸のアッセンブリー
CN102630140A (zh) * 2009-09-15 2012-08-08 蓝宝石能源公司 用于转化栅藻和杜氏藻叶绿体基因组的系统
CN104277974B (zh) * 2013-07-03 2017-09-15 中国科学院植物研究所 一种分离产氢衣藻叶绿体的方法
CN104419701B (zh) * 2013-08-29 2019-01-11 天津大学 多片段dna的酵母快速组装方法
JP6226464B2 (ja) * 2013-09-30 2017-11-08 花王株式会社 チオエステラーゼ改変体を用いた脂質の製造方法
CA2957938A1 (en) * 2014-08-25 2016-03-03 GeneWeave Biosciences, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
US11008602B2 (en) 2017-12-20 2021-05-18 Roche Molecular Systems, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
CN109536525B (zh) * 2019-02-20 2019-08-23 中国科学院烟台海岸带研究所 一种杜氏盐藻叶绿体同源重组空载体及其应用
CN110669789B (zh) * 2019-12-04 2020-04-14 中国科学院烟台海岸带研究所 一种雨生红球藻叶绿体表达系统及其应用
CN111440733A (zh) * 2020-02-07 2020-07-24 天津大学 产松油醇的重组酿酒酵母及构建方法及应用
CN115948450B (zh) * 2022-08-01 2024-02-13 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
CN116218750A (zh) * 2022-10-14 2023-06-06 天津科技大学 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5866404A (en) * 1995-12-06 1999-02-02 Yale University Yeast-bacteria shuttle vector
US5976846A (en) 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
EP2319933B1 (en) * 1997-08-07 2014-10-15 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
WO2000006715A1 (en) * 1998-07-27 2000-02-10 Genotypes Inc. Automatic eukaryotic artificial chromosome vector
WO2000075299A1 (en) * 1999-06-07 2000-12-14 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
BRPI0508271A (pt) * 2004-03-03 2007-08-07 Nat Univ Corp Nara Inst método para aumentar a produtividade de plantas por tecnologia de cloroplasto
WO2005108568A1 (en) * 2004-05-10 2005-11-17 Basf Plant Science Gmbh Methods for assembling multiple expression constructs
SG178735A1 (en) * 2007-06-01 2012-03-29 Scripps Research Inst High throughput screening of genetically modified photosynthetic organisms
US20090209015A1 (en) * 2008-02-15 2009-08-20 Ramesha Chakkodabylu S Compositions and methods for production of biofuels

Also Published As

Publication number Publication date
EP2195426B1 (en) 2013-06-12
GB2453648A8 (en) 2010-11-17
CN101939423A (zh) 2011-01-05
JP2010539979A (ja) 2010-12-24
CA2698627A1 (en) 2009-04-09
EP2195426A4 (en) 2010-12-01
AU2008307471A1 (en) 2009-04-09
IL204250A (en) 2012-12-31
GB0818260D0 (en) 2008-11-12
GB2453648C (en) 2012-12-05
JP5666908B2 (ja) 2015-02-12
GB0913302D0 (en) 2009-09-02
GB2453648B (en) 2011-01-05
DK2195426T3 (da) 2013-09-08
KR20100085930A (ko) 2010-07-29
GB2453648A (en) 2009-04-15
WO2009045550A2 (en) 2009-04-09
GB2460351B (en) 2011-03-30
WO2009045550A3 (en) 2009-07-30
GB2460351A (en) 2009-12-02
GB2453648C2 (en) 2012-12-19
AU2008307471B2 (en) 2013-05-02
EP2195426A2 (en) 2010-06-16
NZ583682A (en) 2012-07-27
KR101389254B1 (ko) 2014-04-24

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