DK2195426T3 - System til opsamling og modificering af store stykker af genomisk DNA og konstruktion af organismer med syntetiske kloroplastre. - Google Patents

System til opsamling og modificering af store stykker af genomisk DNA og konstruktion af organismer med syntetiske kloroplastre.

Info

Publication number
DK2195426T3
DK2195426T3 DK08836521.8T DK08836521T DK2195426T3 DK 2195426 T3 DK2195426 T3 DK 2195426T3 DK 08836521 T DK08836521 T DK 08836521T DK 2195426 T3 DK2195426 T3 DK 2195426T3
Authority
DK
Denmark
Prior art keywords
collecting
genomic dna
large pieces
chloroplasts
modifying large
Prior art date
Application number
DK08836521.8T
Other languages
English (en)
Inventor
Michael Mendez
Bryan O'neill
Kari Mikkelson
Original Assignee
Sapphire Energy Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sapphire Energy Inc filed Critical Sapphire Energy Inc
Application granted granted Critical
Publication of DK2195426T3 publication Critical patent/DK2195426T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK08836521.8T 2007-10-05 2008-10-06 System til opsamling og modificering af store stykker af genomisk DNA og konstruktion af organismer med syntetiske kloroplastre. DK2195426T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97802407P 2007-10-05 2007-10-05
PCT/US2008/011540 WO2009045550A2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts

Publications (1)

Publication Number Publication Date
DK2195426T3 true DK2195426T3 (da) 2013-09-08

Family

ID=40042337

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08836521.8T DK2195426T3 (da) 2007-10-05 2008-10-06 System til opsamling og modificering af store stykker af genomisk DNA og konstruktion af organismer med syntetiske kloroplastre.

Country Status (12)

Country Link
EP (1) EP2195426B1 (da)
JP (1) JP5666908B2 (da)
KR (1) KR101389254B1 (da)
CN (1) CN101939423A (da)
AU (1) AU2008307471B2 (da)
CA (1) CA2698627A1 (da)
DK (1) DK2195426T3 (da)
GB (2) GB2460351B (da)
IL (1) IL204250A (da)
MX (1) MX2010003157A (da)
NZ (1) NZ583682A (da)
WO (1) WO2009045550A2 (da)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5618413B2 (ja) 2007-10-08 2014-11-05 シンセティック ゲノミクス、インク． 大型核酸のアッセンブリー
US20120252054A1 (en) * 2009-09-15 2012-10-04 Sapphire Energy Inc System for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
CN104277974B (zh) * 2013-07-03 2017-09-15 中国科学院植物研究所 一种分离产氢衣藻叶绿体的方法
CN104419701B (zh) * 2013-08-29 2019-01-11 天津大学 多片段dna的酵母快速组装方法
JP6226464B2 (ja) * 2013-09-30 2017-11-08 花王株式会社 チオエステラーゼ改変体を用いた脂質の製造方法
JP2017525377A (ja) * 2014-08-25 2017-09-07 ジーンウィーブ バイオサイエンシズ，インコーポレイティド 非複製的形質導入粒子及び形質導入粒子に基づくレポーターシステム
US11008602B2 (en) 2017-12-20 2021-05-18 Roche Molecular Systems, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
CN109536525B (zh) * 2019-02-20 2019-08-23 中国科学院烟台海岸带研究所 一种杜氏盐藻叶绿体同源重组空载体及其应用
CN110669789B (zh) * 2019-12-04 2020-04-14 中国科学院烟台海岸带研究所 一种雨生红球藻叶绿体表达系统及其应用
CN111440733A (zh) * 2020-02-07 2020-07-24 天津大学 产松油醇的重组酿酒酵母及构建方法及应用
CN115948450B (zh) * 2022-08-01 2024-02-13 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
CN116218750A (zh) * 2022-10-14 2023-06-06 天津科技大学 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5866404A (en) * 1995-12-06 1999-02-02 Yale University Yeast-bacteria shuttle vector
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
JP2002524023A (ja) * 1997-08-07 2002-08-06 オーバーン・ユニバーシティ 万能葉緑体組込および発現ベクター、その形質転換植物ならびに生産物
US6670154B1 (en) * 1998-07-27 2003-12-30 Ronald A. Hitzeman Automatic eukaryotic artificial chromosome vector
WO2000075299A1 (en) * 1999-06-07 2000-12-14 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
WO2005085454A2 (en) * 2004-03-03 2005-09-15 National University Corporation NARA Institute of Science and Technology Method for improving productivity of plant by chloroplast technology
EP1629096A1 (en) * 2004-05-10 2006-03-01 BASF Plant Science GmbH Methods for assembling multiple expression constructs
CN101896603A (zh) * 2007-06-01 2010-11-24 蓝宝石能源公司 使用遗传修饰的生物产生生物质降解酶
WO2009103065A2 (en) * 2008-02-15 2009-08-20 Ramesha Chakkodabylu S Compositions and methods for production of biofuels

Also Published As

Publication number Publication date
WO2009045550A2 (en) 2009-04-09
CA2698627A1 (en) 2009-04-09
GB2453648C (en) 2012-12-05
EP2195426A2 (en) 2010-06-16
GB2453648A8 (en) 2010-11-17
AU2008307471B2 (en) 2013-05-02
GB2453648B (en) 2011-01-05
AU2008307471A1 (en) 2009-04-09
EP2195426A4 (en) 2010-12-01
GB0818260D0 (en) 2008-11-12
GB2460351B (en) 2011-03-30
GB2460351A (en) 2009-12-02
GB2453648C2 (en) 2012-12-19
GB0913302D0 (en) 2009-09-02
IL204250A (en) 2012-12-31
MX2010003157A (es) 2010-05-21
NZ583682A (en) 2012-07-27
JP5666908B2 (ja) 2015-02-12
WO2009045550A3 (en) 2009-07-30
CN101939423A (zh) 2011-01-05
EP2195426B1 (en) 2013-06-12
GB2453648A (en) 2009-04-15
KR101389254B1 (ko) 2014-04-24
KR20100085930A (ko) 2010-07-29
JP2010539979A (ja) 2010-12-24

Similar Documents

Publication Publication Date Title
DK2195426T3 (da) System til opsamling og modificering af store stykker af genomisk DNA og konstruktion af organismer med syntetiske kloroplastre.
DK2195466T3 (da) Fremgangsmåde til opbevaring af biologiske prøver
DK2522717T3 (da) Oligopeptid-frie cellekulturmedier
BRPI0812233A2 (pt) Métodos e dispositivo para substituição exata de dna alvo em organismos eucarióticos.
DK2558579T3 (da) Capping-tilbøjelige rna-polymeraseenzymer og anvendelser deraf
DK2601611T3 (da) Fremgangsmåder til forudsigelse af stabilitet og smeltetemperaturer for nukleinsyreduplekser
DK2129797T3 (da) Nukleinsyrepåvisning
BRPI0923159A2 (pt) gene de delta-endotoxina axmi-150 e métodos para sua utilização.
FR2929502B1 (fr) Implant nucleique.
BRPI0818458A2 (pt) Captura de carbono otimizada em fermentação
DK3192865T3 (da) Enkelt pluripotent stamcelle-kultur
BRPI1012765A2 (pt) sistemas e método de coleta de algas.
DK2195548T3 (da) Leje, og fremgangsmåde og håndtering af lejet
DK1984502T3 (da) Nukleinsyre
DK2143495T3 (da) Container crusher til selektiv indsamling af fast affald og forskellige tilpasninger for hver af de forskellige typer affald, herunder huscontainer-crusher
DK2126602T3 (da) Tilvejebringelse af hjælpedata
ITTO20100791A1 (it) Sistema di innesto a frizione a dischi multipli
BRPI0816776A2 (pt) métodos para usar marcadores genéticos e interações epistáticas relacionadas
BRPI1010843A2 (pt) plantas tolerantes à seca
DK2080811T3 (da) Fremgangsmåde til evaluering af DNA-integriteten af mikroorganismer
DE602007008627D1 (de) Zellkulturmedium
DK2214929T3 (da) Køretøj til håndtering af styrtgods
BRPI0813726A2 (pt) Célula de lítio
DK2200910T3 (da) Affaldsopbevaring
DK2217208T3 (da) Nucleinsyrekompleks og nucleinsyrefremføringssammensætning