MX2010003644A - Ensamble de acidos nucleicos grandes. - Google Patents

Ensamble de acidos nucleicos grandes.

Info

Publication number
MX2010003644A
MX2010003644A MX2010003644A MX2010003644A MX2010003644A MX 2010003644 A MX2010003644 A MX 2010003644A MX 2010003644 A MX2010003644 A MX 2010003644A MX 2010003644 A MX2010003644 A MX 2010003644A MX 2010003644 A MX2010003644 A MX 2010003644A
Authority
MX
Mexico
Prior art keywords
assembly
nucleic acids
large nucleic
cassettes
recombination
Prior art date
Application number
MX2010003644A
Other languages
English (en)
Inventor
J Craig Venter
Daniel G Gibson
Gwynedd A Benders
Lei Young
John I Glass
Clyde A Hutchison Iii
Hamilton O Smith
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of MX2010003644A publication Critical patent/MX2010003644A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se describe un método para ensamblar cualquier molécula de ácido nucleico deseada al combinar casetes in vitro para formar ensambles que además se combinan in vivo, o al ensamblar números grandes de fragmentos de DNA por recombinación en un cultivo de levadura para obtener moléculas de DNA deseadas de tamaño sustancial.
MX2010003644A 2007-10-08 2008-10-07 Ensamble de acidos nucleicos grandes. MX2010003644A (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US97838807P 2007-10-08 2007-10-08
US98354907P 2007-10-29 2007-10-29
US6221408P 2008-01-23 2008-01-23
US2339208P 2008-01-24 2008-01-24
US9627008P 2008-09-11 2008-09-11
PCT/US2008/079109 WO2009048885A2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Publications (1)

Publication Number Publication Date
MX2010003644A true MX2010003644A (es) 2010-06-23

Family

ID=40549815

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010003644A MX2010003644A (es) 2007-10-08 2008-10-07 Ensamble de acidos nucleicos grandes.

Country Status (11)

Country Link
US (1) US9593329B2 (es)
EP (1) EP2207899B1 (es)
JP (1) JP5618413B2 (es)
CN (1) CN101932725A (es)
AU (1) AU2008311000B2 (es)
CA (1) CA2701923C (es)
DK (1) DK2207899T3 (es)
IL (1) IL204892B (es)
MX (1) MX2010003644A (es)
MY (1) MY161418A (es)
WO (1) WO2009048885A2 (es)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US9234226B2 (en) 2008-10-14 2016-01-12 Wayne M. Barnes In vitro recombination methods using 5′ exonuclease
BRPI0922187B1 (pt) 2008-11-19 2021-05-04 Amyris, Inc Métodos para gerar um polinucleotídeo e para gerar uma célula hospedeira
CN105274004B (zh) 2009-03-06 2019-08-09 合成基因组股份有限公司 用于克隆和操作基因组的方法
CN104862335A (zh) 2009-07-31 2015-08-26 诺华股份有限公司 反向遗传系统
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
EP2542676A1 (en) * 2010-03-05 2013-01-09 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2012051327A2 (en) 2010-10-12 2012-04-19 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
EP3964285A1 (en) 2011-09-26 2022-03-09 Thermo Fisher Scientific Geneart GmbH High efficiency, small volume nucleic acid synthesis
CN103975063A (zh) * 2011-11-23 2014-08-06 帝斯曼知识产权资产管理有限公司 核酸组装系统
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
WO2014036404A2 (en) * 2012-08-31 2014-03-06 Synthetic Genomics, Inc. Crowding agent-induced nucleic acid transfer into a recipient host cell
DK2931913T3 (en) * 2012-12-13 2018-11-26 Synthetic Genomics Inc PEG-MEDIATED COMPOSITION OF NUCLEIC ACID MOLECULES
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
BR112015017420A2 (pt) * 2013-01-23 2017-07-11 Novartis Ag recombinação de vírus influenza
US20170198268A1 (en) 2014-07-09 2017-07-13 Gen9, Inc. Compositions and Methods for Site-Directed DNA Nicking and Cleaving
LT3557262T (lt) 2014-12-09 2022-11-10 Life Technologies Corporation Didelio efektyvumo nukleorūgščių sintezė mažame tūryje
CN106282224B (zh) * 2015-06-26 2020-05-01 深圳华大生命科学研究院 在靶细胞中构建人工染色体的方法以及真核细胞
EP3359669B1 (en) 2015-10-06 2020-05-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
CA3018199A1 (en) 2016-03-23 2017-09-28 Synthetic Genomics, Inc. Generation of synthetic genomes
EP3541954A1 (en) * 2016-11-21 2019-09-25 Novozymes A/S Yeast cell extract assisted construction of dna molecules
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
EP3814494B1 (en) 2018-06-29 2023-11-01 Thermo Fisher Scientific GENEART GmbH High throughput assembly of nucleic acid molecules
WO2020020608A1 (en) * 2018-07-23 2020-01-30 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
WO2020224987A1 (en) * 2019-05-06 2020-11-12 Dsm Ip Assets B.V. Multipartite crispr donor
EP4114972A1 (en) 2020-03-06 2023-01-11 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN113380322B (zh) * 2021-06-25 2023-10-24 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法

Family Cites Families (13)

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US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
AU6487196A (en) 1996-07-09 1998-02-02 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Transformation-associated recombination cloning
DE69830065T2 (de) * 1997-09-16 2006-01-19 Egea Biosciences, LLC, San Diego Methoden zur kompletten chemischen synthese und zusammensetzung von genen und genomen
US6670154B1 (en) * 1998-07-27 2003-12-30 Ronald A. Hitzeman Automatic eukaryotic artificial chromosome vector
US7083971B1 (en) * 1999-06-07 2006-08-01 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
US7822969B2 (en) 2001-04-16 2010-10-26 Digimarc Corporation Watermark systems and methods
US20040248289A1 (en) * 2001-10-04 2004-12-09 Vladimir Noskov Tandem repeat markers
WO2004113534A1 (en) * 2003-05-22 2004-12-29 University Of California Method for producing a synthetic gene or other dna sequence
MY143596A (en) * 2005-08-11 2011-06-15 Synthetic Genomics Inc In vitro recombination method
CA2618665C (en) * 2005-08-11 2012-11-13 J. Craig Venter Institute Method for in vitro recombination
JP2009511051A (ja) 2005-10-12 2009-03-19 ザ ジェイ． クレイグ ベンター インスティテュート 最小限の細菌ゲノム
NZ583682A (en) 2007-10-05 2012-07-27 Sapphire Energy Inc System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts

Also Published As

Publication number Publication date
IL204892A0 (en) 2010-11-30
AU2008311000A1 (en) 2009-04-16
AU2008311000A2 (en) 2010-06-03
CA2701923A1 (en) 2009-04-16
EP2207899A4 (en) 2010-12-01
JP2010539994A (ja) 2010-12-24
JP5618413B2 (ja) 2014-11-05
US9593329B2 (en) 2017-03-14
CN101932725A (zh) 2010-12-29
WO2009048885A2 (en) 2009-04-16
AU2008311000B2 (en) 2013-12-19
WO2009048885A3 (en) 2009-12-30
IL204892B (en) 2018-02-28
EP2207899A2 (en) 2010-07-21
MY161418A (en) 2017-04-14
DK2207899T3 (en) 2018-09-03
CA2701923C (en) 2018-07-10
EP2207899B1 (en) 2018-04-18
US20090275086A1 (en) 2009-11-05

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