JP5666908B2 - ゲノムｄｎａの大きな断片の捕捉および修飾、ならびに合成葉緑体を有する生物を構築するための系 - Google Patents

ゲノムｄｎａの大きな断片の捕捉および修飾、ならびに合成葉緑体を有する生物を構築するための系 Download PDF

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JP5666908B2
JP5666908B2 JP2010528006A JP2010528006A JP5666908B2 JP 5666908 B2 JP5666908 B2 JP 5666908B2 JP 2010528006 A JP2010528006 A JP 2010528006A JP 2010528006 A JP2010528006 A JP 2010528006A JP 5666908 B2 JP5666908 B2 JP 5666908B2
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yeast
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vectors
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chloroplast
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JP2010539979A5 (enExample
JP2010539979A (ja
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メンデス，マイケル
オニール，ブライアン
ミッケルソン，カリ
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サファイア エナジー，インコーポレイティド
サファイア エナジー，インコーポレイティド
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

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JP2010528006A 2007-10-05 2008-10-06 ゲノムｄｎａの大きな断片の捕捉および修飾、ならびに合成葉緑体を有する生物を構築するための系 Expired - Fee Related JP5666908B2 (ja)

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Application Number Priority Date Filing Date Title
US97802407P 2007-10-05 2007-10-05
US60/978,024 2007-10-05
PCT/US2008/011540 WO2009045550A2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts

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JP2010539979A JP2010539979A (ja) 2010-12-24
JP2010539979A5 JP2010539979A5 (enExample) 2011-11-24
JP5666908B2 true JP5666908B2 (ja) 2015-02-12

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EP (1) EP2195426B1 (enExample)
JP (1) JP5666908B2 (enExample)
KR (1) KR101389254B1 (enExample)
CN (1) CN101939423A (enExample)
AU (1) AU2008307471B2 (enExample)
CA (1) CA2698627A1 (enExample)
DK (1) DK2195426T3 (enExample)
GB (2) GB2460351B (enExample)
IL (1) IL204250A (enExample)
MX (1) MX2010003157A (enExample)
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MX2010003644A (es) 2007-10-08 2010-06-23 Synthetic Genomics Inc Ensamble de acidos nucleicos grandes.
CA2773675A1 (en) * 2009-09-15 2011-03-24 Sapphire Energy, Inc. A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
CN104277974B (zh) * 2013-07-03 2017-09-15 中国科学院植物研究所 一种分离产氢衣藻叶绿体的方法
CN104419701B (zh) * 2013-08-29 2019-01-11 天津大学 多片段dna的酵母快速组装方法
JP6226464B2 (ja) * 2013-09-30 2017-11-08 花王株式会社 チオエステラーゼ改変体を用いた脂質の製造方法
KR101946180B1 (ko) 2014-08-25 2019-02-08 지니위브 바이오사이언시스, 인코포레이티드 비-복제 형질도입 입자 및 형질도입 입자-기반 리포터 시스템
US11008602B2 (en) 2017-12-20 2021-05-18 Roche Molecular Systems, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
CN109536525B (zh) * 2019-02-20 2019-08-23 中国科学院烟台海岸带研究所 一种杜氏盐藻叶绿体同源重组空载体及其应用
CN110669789B (zh) * 2019-12-04 2020-04-14 中国科学院烟台海岸带研究所 一种雨生红球藻叶绿体表达系统及其应用
CN111440733A (zh) * 2020-02-07 2020-07-24 天津大学 产松油醇的重组酿酒酵母及构建方法及应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
CN115948450B (zh) * 2022-08-01 2024-02-13 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
CN116218750B (zh) * 2022-10-14 2024-11-26 天津科技大学 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用
CN118256412B (zh) * 2024-04-01 2024-09-27 湖北大学 运动发酵单胞菌的工程菌株、制备方法及应用

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Publication number Priority date Publication date Assignee Title
US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5866404A (en) * 1995-12-06 1999-02-02 Yale University Yeast-bacteria shuttle vector
US5976846A (en) 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
NZ502742A (en) * 1997-08-07 2004-04-30 Univ Auburn Universal chloroplast integration and expression vectors, transformed plants and products thereof
AU5217599A (en) * 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
CA2336590A1 (en) * 1999-06-07 2000-12-14 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
CN1997743B (zh) * 2004-03-03 2011-12-21 国立大学法人奈良先端科学技术大学院大学 通过叶绿体技术提高植物生产能力的方法
EP1629096A1 (en) * 2004-05-10 2006-03-01 BASF Plant Science GmbH Methods for assembling multiple expression constructs
DK2162537T3 (da) * 2007-06-01 2012-04-23 Sapphire Energy Inc Anvendelse af genetisk modificerede organismer til at frembringe biomasse-nedbrydende enzymer.
WO2009103065A2 (en) * 2008-02-15 2009-08-20 Ramesha Chakkodabylu S Compositions and methods for production of biofuels

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DK2195426T3 (da) 2013-09-08
WO2009045550A3 (en) 2009-07-30
EP2195426A2 (en) 2010-06-16
KR101389254B1 (ko) 2014-04-24
GB2453648C (en) 2012-12-05
JP2010539979A (ja) 2010-12-24
CA2698627A1 (en) 2009-04-09
GB2460351A (en) 2009-12-02
GB2453648B (en) 2011-01-05
WO2009045550A2 (en) 2009-04-09
MX2010003157A (es) 2010-05-21
NZ583682A (en) 2012-07-27
EP2195426A4 (en) 2010-12-01
CN101939423A (zh) 2011-01-05
AU2008307471A1 (en) 2009-04-09
IL204250A (en) 2012-12-31
GB0913302D0 (en) 2009-09-02
GB2460351B (en) 2011-03-30
EP2195426B1 (en) 2013-06-12
GB2453648C2 (en) 2012-12-19
GB0818260D0 (en) 2008-11-12
GB2453648A (en) 2009-04-15
GB2453648A8 (en) 2010-11-17
AU2008307471B2 (en) 2013-05-02
KR20100085930A (ko) 2010-07-29

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