AU2008307471B2 - System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts - Google Patents

System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts Download PDF

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Publication number
AU2008307471B2
AU2008307471B2 AU2008307471A AU2008307471A AU2008307471B2 AU 2008307471 B2 AU2008307471 B2 AU 2008307471B2 AU 2008307471 A AU2008307471 A AU 2008307471A AU 2008307471 A AU2008307471 A AU 2008307471A AU 2008307471 B2 AU2008307471 B2 AU 2008307471B2
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AU
Australia
Prior art keywords
dna
yeast
vector
chloroplast
vectors
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Ceased
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AU2008307471A
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English (en)
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AU2008307471A1 (en
Inventor
Mike Mendez
Kari Mikkelson
Bryan O'neill
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Renew Biopharma Inc
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Renew Biopharma Inc
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Publication of AU2008307471A1 publication Critical patent/AU2008307471A1/en
Priority to AU2013203045A priority Critical patent/AU2013203045B2/en
Application granted granted Critical
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Assigned to RENEW BIOPHARMA, INC. reassignment RENEW BIOPHARMA, INC. Request for Assignment Assignors: SAPPHIRE ENERGY, INC.
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2008307471A 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts Ceased AU2008307471B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2013203045A AU2013203045B2 (en) 2007-10-05 2013-04-03 System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US97802407P 2007-10-05 2007-10-05
US60/978,024 2007-10-05
PCT/US2008/011540 WO2009045550A2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2013203045A Division AU2013203045B2 (en) 2007-10-05 2013-04-03 System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts

Publications (2)

Publication Number Publication Date
AU2008307471A1 AU2008307471A1 (en) 2009-04-09
AU2008307471B2 true AU2008307471B2 (en) 2013-05-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008307471A Ceased AU2008307471B2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts

Country Status (12)

Country Link
EP (1) EP2195426B1 (enExample)
JP (1) JP5666908B2 (enExample)
KR (1) KR101389254B1 (enExample)
CN (1) CN101939423A (enExample)
AU (1) AU2008307471B2 (enExample)
CA (1) CA2698627A1 (enExample)
DK (1) DK2195426T3 (enExample)
GB (2) GB2460351B (enExample)
IL (1) IL204250A (enExample)
MX (1) MX2010003157A (enExample)
NZ (1) NZ583682A (enExample)
WO (1) WO2009045550A2 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2010003644A (es) 2007-10-08 2010-06-23 Synthetic Genomics Inc Ensamble de acidos nucleicos grandes.
CA2773675A1 (en) * 2009-09-15 2011-03-24 Sapphire Energy, Inc. A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
CN104277974B (zh) * 2013-07-03 2017-09-15 中国科学院植物研究所 一种分离产氢衣藻叶绿体的方法
CN104419701B (zh) * 2013-08-29 2019-01-11 天津大学 多片段dna的酵母快速组装方法
JP6226464B2 (ja) * 2013-09-30 2017-11-08 花王株式会社 チオエステラーゼ改変体を用いた脂質の製造方法
KR101946180B1 (ko) 2014-08-25 2019-02-08 지니위브 바이오사이언시스, 인코포레이티드 비-복제 형질도입 입자 및 형질도입 입자-기반 리포터 시스템
US11008602B2 (en) 2017-12-20 2021-05-18 Roche Molecular Systems, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
CN109536525B (zh) * 2019-02-20 2019-08-23 中国科学院烟台海岸带研究所 一种杜氏盐藻叶绿体同源重组空载体及其应用
CN110669789B (zh) * 2019-12-04 2020-04-14 中国科学院烟台海岸带研究所 一种雨生红球藻叶绿体表达系统及其应用
CN111440733A (zh) * 2020-02-07 2020-07-24 天津大学 产松油醇的重组酿酒酵母及构建方法及应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
CN115948450B (zh) * 2022-08-01 2024-02-13 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
CN116218750B (zh) * 2022-10-14 2024-11-26 天津科技大学 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用
CN118256412B (zh) * 2024-04-01 2024-09-27 湖北大学 运动发酵单胞菌的工程菌株、制备方法及应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005108568A1 (en) * 2004-05-10 2005-11-17 Basf Plant Science Gmbh Methods for assembling multiple expression constructs

Family Cites Families (9)

* Cited by examiner, † Cited by third party
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US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5866404A (en) * 1995-12-06 1999-02-02 Yale University Yeast-bacteria shuttle vector
US5976846A (en) 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
NZ502742A (en) * 1997-08-07 2004-04-30 Univ Auburn Universal chloroplast integration and expression vectors, transformed plants and products thereof
AU5217599A (en) * 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
CA2336590A1 (en) * 1999-06-07 2000-12-14 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
CN1997743B (zh) * 2004-03-03 2011-12-21 国立大学法人奈良先端科学技术大学院大学 通过叶绿体技术提高植物生产能力的方法
DK2162537T3 (da) * 2007-06-01 2012-04-23 Sapphire Energy Inc Anvendelse af genetisk modificerede organismer til at frembringe biomasse-nedbrydende enzymer.
WO2009103065A2 (en) * 2008-02-15 2009-08-20 Ramesha Chakkodabylu S Compositions and methods for production of biofuels

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005108568A1 (en) * 2004-05-10 2005-11-17 Basf Plant Science Gmbh Methods for assembling multiple expression constructs

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Gupta M et al, Plant Molecular Biology, 1991, 17(3):361-369 *

Also Published As

Publication number Publication date
DK2195426T3 (da) 2013-09-08
WO2009045550A3 (en) 2009-07-30
EP2195426A2 (en) 2010-06-16
KR101389254B1 (ko) 2014-04-24
GB2453648C (en) 2012-12-05
JP2010539979A (ja) 2010-12-24
CA2698627A1 (en) 2009-04-09
GB2460351A (en) 2009-12-02
GB2453648B (en) 2011-01-05
WO2009045550A2 (en) 2009-04-09
MX2010003157A (es) 2010-05-21
NZ583682A (en) 2012-07-27
EP2195426A4 (en) 2010-12-01
CN101939423A (zh) 2011-01-05
AU2008307471A1 (en) 2009-04-09
IL204250A (en) 2012-12-31
GB0913302D0 (en) 2009-09-02
GB2460351B (en) 2011-03-30
EP2195426B1 (en) 2013-06-12
JP5666908B2 (ja) 2015-02-12
GB2453648C2 (en) 2012-12-19
GB0818260D0 (en) 2008-11-12
GB2453648A (en) 2009-04-15
GB2453648A8 (en) 2010-11-17
KR20100085930A (ko) 2010-07-29

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Owner name: RENEW BIOPHARMA, INC.

Free format text: FORMER OWNER(S): SAPPHIRE ENERGY, INC.

MK14 Patent ceased section 143(a) (annual fees not paid) or expired