GB2460351B - System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts - Google Patents

System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts

Info

Publication number
GB2460351B
GB2460351B GB0913302A GB0913302A GB2460351B GB 2460351 B GB2460351 B GB 2460351B GB 0913302 A GB0913302 A GB 0913302A GB 0913302 A GB0913302 A GB 0913302A GB 2460351 B GB2460351 B GB 2460351B
Authority
GB
United Kingdom
Prior art keywords
capturing
genomic dna
large pieces
chloroplasts
modifying large
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB0913302A
Other languages
English (en)
Other versions
GB2460351A (en
GB0913302D0 (en
Inventor
Michael Mendez
Bryan O'neill
Kari Mikkelson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sapphire Energy Inc
Original Assignee
Sapphire Energy Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sapphire Energy Inc filed Critical Sapphire Energy Inc
Priority to GB0818260A priority Critical patent/GB2453648C2/en
Priority to GB1100928.9A priority patent/GB2475435B/en
Publication of GB0913302D0 publication Critical patent/GB0913302D0/en
Publication of GB2460351A publication Critical patent/GB2460351A/en
Application granted granted Critical
Publication of GB2460351B publication Critical patent/GB2460351B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB0913302A 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts Expired - Fee Related GB2460351B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB0818260A GB2453648C2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces ofgenomic DNA and constructing organisms with synth etic chloroplasts
GB1100928.9A GB2475435B (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97802407P 2007-10-05 2007-10-05
GB0818260A GB2453648C2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces ofgenomic DNA and constructing organisms with synth etic chloroplasts

Publications (3)

Publication Number Publication Date
GB0913302D0 GB0913302D0 (en) 2009-09-02
GB2460351A GB2460351A (en) 2009-12-02
GB2460351B true GB2460351B (en) 2011-03-30

Family

ID=40042337

Family Applications (2)

Application Number Title Priority Date Filing Date
GB0913302A Expired - Fee Related GB2460351B (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts
GB0818260A Expired - Fee Related GB2453648C2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces ofgenomic DNA and constructing organisms with synth etic chloroplasts

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB0818260A Expired - Fee Related GB2453648C2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces ofgenomic DNA and constructing organisms with synth etic chloroplasts

Country Status (12)

Country Link
EP (1) EP2195426B1 (enExample)
JP (1) JP5666908B2 (enExample)
KR (1) KR101389254B1 (enExample)
CN (1) CN101939423A (enExample)
AU (1) AU2008307471B2 (enExample)
CA (1) CA2698627A1 (enExample)
DK (1) DK2195426T3 (enExample)
GB (2) GB2460351B (enExample)
IL (1) IL204250A (enExample)
MX (1) MX2010003157A (enExample)
NZ (1) NZ583682A (enExample)
WO (1) WO2009045550A2 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2010003644A (es) 2007-10-08 2010-06-23 Synthetic Genomics Inc Ensamble de acidos nucleicos grandes.
CA2773675A1 (en) * 2009-09-15 2011-03-24 Sapphire Energy, Inc. A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
CN104277974B (zh) * 2013-07-03 2017-09-15 中国科学院植物研究所 一种分离产氢衣藻叶绿体的方法
CN104419701B (zh) * 2013-08-29 2019-01-11 天津大学 多片段dna的酵母快速组装方法
JP6226464B2 (ja) * 2013-09-30 2017-11-08 花王株式会社 チオエステラーゼ改変体を用いた脂質の製造方法
KR101946180B1 (ko) 2014-08-25 2019-02-08 지니위브 바이오사이언시스, 인코포레이티드 비-복제 형질도입 입자 및 형질도입 입자-기반 리포터 시스템
US11008602B2 (en) 2017-12-20 2021-05-18 Roche Molecular Systems, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
CN109536525B (zh) * 2019-02-20 2019-08-23 中国科学院烟台海岸带研究所 一种杜氏盐藻叶绿体同源重组空载体及其应用
CN110669789B (zh) * 2019-12-04 2020-04-14 中国科学院烟台海岸带研究所 一种雨生红球藻叶绿体表达系统及其应用
CN111440733A (zh) * 2020-02-07 2020-07-24 天津大学 产松油醇的重组酿酒酵母及构建方法及应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
CN115948450B (zh) * 2022-08-01 2024-02-13 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
CN116218750B (zh) * 2022-10-14 2024-11-26 天津科技大学 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用
CN118256412B (zh) * 2024-04-01 2024-09-27 湖北大学 运动发酵单胞菌的工程菌株、制备方法及应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US20020132348A1 (en) * 1995-12-06 2002-09-19 Bradshaw M. Suzanne New yeast-bacteria shuttle vector
US20050019924A1 (en) * 1998-07-27 2005-01-27 Hitzeman Ronald A. Automatic eukaryotic artificial chromosome vector
WO2005085454A2 (en) * 2004-03-03 2005-09-15 National University Corporation NARA Institute of Science and Technology Method for improving productivity of plant by chloroplast technology
WO2005108568A1 (en) * 2004-05-10 2005-11-17 Basf Plant Science Gmbh Methods for assembling multiple expression constructs
WO2009103065A2 (en) * 2008-02-15 2009-08-20 Ramesha Chakkodabylu S Compositions and methods for production of biofuels

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5976846A (en) 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
CA2336590A1 (en) * 1999-06-07 2000-12-14 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
DK2162537T3 (da) * 2007-06-01 2012-04-23 Sapphire Energy Inc Anvendelse af genetisk modificerede organismer til at frembringe biomasse-nedbrydende enzymer.

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020132348A1 (en) * 1995-12-06 2002-09-19 Bradshaw M. Suzanne New yeast-bacteria shuttle vector
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US20050019924A1 (en) * 1998-07-27 2005-01-27 Hitzeman Ronald A. Automatic eukaryotic artificial chromosome vector
WO2005085454A2 (en) * 2004-03-03 2005-09-15 National University Corporation NARA Institute of Science and Technology Method for improving productivity of plant by chloroplast technology
WO2005108568A1 (en) * 2004-05-10 2005-11-17 Basf Plant Science Gmbh Methods for assembling multiple expression constructs
WO2009103065A2 (en) * 2008-02-15 2009-08-20 Ramesha Chakkodabylu S Compositions and methods for production of biofuels

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Acta Genetica Sinica, Vol. 13, 1986, Li et al, 'Molecular cloning of the 4.1 kilobase Sal-1 fragment of the spinach chloroplast DNA', pp. 11-16, English Abstract only *
Molecular and General Genetics, Vol. 195, 1984, Ohtani et al, 'Location and nucleotide sequence of a tobacco chloroplast DNA segment capable of replication in yeast', pp. 1-4 *
Plant Molecular Biology, Vol. 17, 1991, Gupta et al, 'Entire maize chloroplast genome is stably maintained in a yeast artificial chromosome', pp. 361-369 *
PNAS, Vol. 78, 1981, Erion et al, 'Cloning, mapping, and in vitro transcription-translation of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from spinach chloroplasts', pp. 3459-3463 *

Also Published As

Publication number Publication date
DK2195426T3 (da) 2013-09-08
WO2009045550A3 (en) 2009-07-30
EP2195426A2 (en) 2010-06-16
KR101389254B1 (ko) 2014-04-24
GB2453648C (en) 2012-12-05
JP2010539979A (ja) 2010-12-24
CA2698627A1 (en) 2009-04-09
GB2460351A (en) 2009-12-02
GB2453648B (en) 2011-01-05
WO2009045550A2 (en) 2009-04-09
MX2010003157A (es) 2010-05-21
NZ583682A (en) 2012-07-27
EP2195426A4 (en) 2010-12-01
CN101939423A (zh) 2011-01-05
AU2008307471A1 (en) 2009-04-09
IL204250A (en) 2012-12-31
GB0913302D0 (en) 2009-09-02
EP2195426B1 (en) 2013-06-12
JP5666908B2 (ja) 2015-02-12
GB2453648C2 (en) 2012-12-19
GB0818260D0 (en) 2008-11-12
GB2453648A (en) 2009-04-15
GB2453648A8 (en) 2010-11-17
AU2008307471B2 (en) 2013-05-02
KR20100085930A (ko) 2010-07-29

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20181006