CN101918559A - 修饰植物中的糖蛋白产生 - Google Patents

修饰植物中的糖蛋白产生 Download PDF

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Publication number
CN101918559A
CN101918559A CN2008801031743A CN200880103174A CN101918559A CN 101918559 A CN101918559 A CN 101918559A CN 2008801031743 A CN2008801031743 A CN 2008801031743A CN 200880103174 A CN200880103174 A CN 200880103174A CN 101918559 A CN101918559 A CN 101918559A
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China
Prior art keywords
nucleotide sequence
plant
protein
interest
seq
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Pending
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CN2008801031743A
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Chinese (zh)
Inventor
马克-安德烈·德奥斯特
埃斯泰勒·马凯-布卢安
穆里尔·巴尔多
卡罗勒·比雷尔
卢瓦克·法耶
帕特里斯·勒鲁热
路易斯-菲利普·韦齐纳
韦罗妮克·戈莫尔德
斯特凡妮·阿奎
克里斯托夫·里韦
托马斯·帕卡莱特
克里斯托夫·苏鲁耶
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medicago Inc
Centre National de la Recherche Scientifique CNRS
Universite de Rouen
Original Assignee
Medicago Inc
Centre National de la Recherche Scientifique CNRS
Universite de Rouen
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Application filed by Medicago Inc, Centre National de la Recherche Scientifique CNRS, Universite de Rouen filed Critical Medicago Inc
Priority to CN201510364867.XA priority Critical patent/CN104962579A/zh
Publication of CN101918559A publication Critical patent/CN101918559A/zh
Pending legal-status Critical Current

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nutrition Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cultivation Of Plants (AREA)
CN2008801031743A 2007-06-15 2008-06-13 修饰植物中的糖蛋白产生 Pending CN101918559A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510364867.XA CN104962579A (zh) 2007-06-15 2008-06-13 修饰植物中的糖蛋白产生

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94434407P 2007-06-15 2007-06-15
US60/944,344 2007-06-15
PCT/CA2008/001139 WO2008151440A1 (en) 2007-06-15 2008-06-13 Modifying glycoprotein production in plants

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN201510364867.XA Division CN104962579A (zh) 2007-06-15 2008-06-13 修饰植物中的糖蛋白产生
CN2013101091944A Division CN103382487A (zh) 2007-06-15 2008-06-13 修饰植物中的糖蛋白产生

Publications (1)

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CN101918559A true CN101918559A (zh) 2010-12-15

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Family Applications (3)

Application Number Title Priority Date Filing Date
CN2008801031743A Pending CN101918559A (zh) 2007-06-15 2008-06-13 修饰植物中的糖蛋白产生
CN201510364867.XA Pending CN104962579A (zh) 2007-06-15 2008-06-13 修饰植物中的糖蛋白产生
CN2013101091944A Pending CN103382487A (zh) 2007-06-15 2008-06-13 修饰植物中的糖蛋白产生

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CN201510364867.XA Pending CN104962579A (zh) 2007-06-15 2008-06-13 修饰植物中的糖蛋白产生
CN2013101091944A Pending CN103382487A (zh) 2007-06-15 2008-06-13 修饰植物中的糖蛋白产生

Country Status (24)

Country Link
US (1) US20110008837A1 (enExample)
EP (1) EP2155880B1 (enExample)
JP (1) JP5783720B2 (enExample)
KR (2) KR20130075787A (enExample)
CN (3) CN101918559A (enExample)
AU (1) AU2008261527C1 (enExample)
BR (1) BRPI0812538B1 (enExample)
CA (2) CA2795379A1 (enExample)
DK (1) DK2155880T3 (enExample)
EG (1) EG26600A (enExample)
ES (1) ES2624776T3 (enExample)
HU (1) HUE031698T2 (enExample)
IL (1) IL202568A (enExample)
MA (1) MA31516B1 (enExample)
MX (1) MX2009013665A (enExample)
NZ (2) NZ598417A (enExample)
PL (1) PL2155880T3 (enExample)
PT (1) PT2155880T (enExample)
RU (2) RU2499053C2 (enExample)
SG (1) SG185249A1 (enExample)
SI (1) SI2155880T1 (enExample)
TN (1) TN2009000512A1 (enExample)
WO (1) WO2008151440A1 (enExample)
ZA (1) ZA201000207B (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
EP2238253B1 (en) 2007-11-27 2012-09-12 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
CA2730171C (en) 2008-07-08 2016-10-11 Medicago Inc. Soluble recombinant influenza antigens
ES2593277T3 (es) 2008-07-18 2016-12-07 Medicago Inc. Nuevo epítopo de inmunización contra el virus de la influenza
NZ597401A (en) 2009-06-24 2013-09-27 Medicago Inc Chimeric influenza virus-like particles comprising hemagglutinin
DK2480560T3 (en) 2009-09-22 2018-04-16 Medicago Inc PROCEDURE FOR THE PREPARATION OF PLANT DERIVATIVE PROTEINS
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
CA2839932C (en) * 2011-06-07 2022-07-05 Ibio, Inc. In vivo de-glycosylation of recombinant proteins by co-expression with pngase f
EP2551348B1 (en) 2011-07-29 2014-09-24 Icon Genetics GmbH Production of galactosylated N-glycans in plants
CN116218900A (zh) 2011-09-30 2023-06-06 麦迪卡格公司 增加植物中病毒样颗粒的产率
WO2013169009A1 (ko) * 2012-05-08 2013-11-14 경상대학교 산학협력단 고-만노오스형 n-글라이칸을 생산하는 식물체 및 이를 이용한 고-만노오스형 n-글라이칸의 생산방법
ES2803508T3 (es) 2013-03-28 2021-01-27 Medicago Inc Producción de partículas similares al virus de la gripe en plantas
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
US11959088B2 (en) 2014-07-11 2024-04-16 Aramis Biotechnologies Inc. Modifying protein production in plants
CN107810271B (zh) * 2014-11-20 2021-09-28 耶路撒冷希伯来大学伊萨姆研发公司 用于在植物细胞中生产具有改变的糖基化模式的多肽的组合物和方法
US11499160B2 (en) 2016-12-01 2022-11-15 Plantform Corporation Transgenic plant with reduced fucosyltransferase and xylosyltransferase activity
US11649465B2 (en) * 2017-09-11 2023-05-16 R.J. Reynolds Tobacco Company Methods and compositions for increasing expression of genes of interest in a plant by co-expression with p21
EP4265104A4 (en) * 2021-01-15 2024-12-11 Denka Company Limited METHOD FOR DETERMINING CULTURE CONDITIONS, AND METHOD FOR PRODUCING DESIRED PROTEIN OR DESIRED PEPTIDE

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5312746A (en) * 1993-01-08 1994-05-17 Life Technologies, Inc. Cloning and expressing restriction endonucleases and modification methylases from caryophanon
GB9524350D0 (en) * 1995-11-29 1996-01-31 Lynxvale Ltd Enhancer-increased gene expression in plants
US5945326A (en) * 1997-03-20 1999-08-31 New England Biolabs, Inc. Method for cloning and producing the Spel restriction endonuclease
US20030175884A1 (en) * 2001-08-03 2003-09-18 Pablo Umana Antibody glycosylation variants having increased antibody-dependent cellular cytotoxicity
DK1137789T3 (da) * 1998-12-09 2010-11-08 Phyton Holdings Llc Fremgangsmåde til fremstilling af et glycoprotein med glycosylering af human type
MXPA02003455A (es) * 1999-10-04 2002-10-23 Medicago Inc Promotor para regular la expresion de genes foraneos.
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
CA2388432A1 (en) * 1999-10-21 2001-04-26 Monsanto Company Post-translational modification of recombinant proteins produced in plants
AU1738301A (en) * 1999-10-26 2001-05-08 Id-Lelystad, Instituut Voor Dierhouderij En Diergezondheid B.V. Gonadotrophins in plants
DK1772518T3 (da) * 1999-10-26 2010-11-22 Stichting Dienst Landbouwkundi Pattedyrsagtig glykosylering i planter
US20040214273A1 (en) * 2001-01-19 2004-10-28 Kazuhito Fujiyama Method for secretory production of glycoprotein having human-type sugar chain using plant cell
US20030035774A1 (en) 2001-07-18 2003-02-20 Adjei Akwete L. Salt/ion pair medicinal aerosol formulation
ES2563402T3 (es) * 2002-02-13 2016-03-15 Wisconsin Alumni Research Foundation Señal para el empaquetamiento de vectores del virus de la gripe
KR101060361B1 (ko) * 2002-03-19 2011-08-29 스티칭 디엔스트 랜드보위쿤디그 온데조에크 식물에서 글리칸 프로세싱의 최적화
WO2003078614A2 (en) * 2002-03-19 2003-09-25 Plant Research International B.V. Gntiii (udp-n-acethylglucosamine:beta-d mannoside beta (1,4)-n-acethylglucosaminy ltransferase iii) expression in plants
CN1761746B (zh) * 2003-01-22 2016-03-23 罗氏格黎卡特股份公司 融合构建体及其用来生产Fc受体结合亲和性和效应子功能提高的抗体的用途
PL222220B1 (pl) 2003-01-22 2016-07-29 Glycart Biotechnology Ag Komórka gospodarza i sposób wytwarzania polipeptydu w komórce gospodarza
WO2004098533A2 (en) * 2003-05-05 2004-11-18 Boyce Thompson Institute For Plant Research Vectors and cells for preparing immunoprotective compositions derived from transgenic plants
JP2006212019A (ja) * 2004-04-30 2006-08-17 National Institute Of Agrobiological Sciences 植物を用いたユビキノン−１０の製造方法

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Publication number Publication date
TN2009000512A1 (en) 2011-03-31
AU2008261527A8 (en) 2010-01-21
CA2700180A1 (en) 2008-12-18
ES2624776T3 (es) 2017-07-17
MA31516B1 (fr) 2010-07-01
PL2155880T3 (pl) 2017-06-30
CA2795379A1 (en) 2008-12-18
NZ598417A (en) 2013-06-28
BRPI0812538A2 (pt) 2014-09-30
AU2008261527A1 (en) 2008-12-18
AU2008261527B8 (en) 2014-04-24
EP2155880A4 (en) 2010-08-04
JP5783720B2 (ja) 2015-09-24
JP2010531136A (ja) 2010-09-24
RU2010101024A (ru) 2011-07-20
SI2155880T1 (sl) 2017-03-31
AU2008261527B2 (en) 2014-04-10
RU2499053C2 (ru) 2013-11-20
AU2008261527C1 (en) 2014-09-04
ZA201000207B (en) 2013-09-25
MX2009013665A (es) 2010-06-02
KR101385050B1 (ko) 2014-05-13
KR20100037602A (ko) 2010-04-09
SG185249A1 (en) 2012-11-29
DK2155880T3 (en) 2016-12-05
PT2155880T (pt) 2016-11-18
EP2155880B1 (en) 2016-08-10
BRPI0812538B1 (pt) 2021-03-16
CA2700180C (en) 2013-01-29
KR20130075787A (ko) 2013-07-05
EG26600A (en) 2014-03-23
WO2008151440A8 (en) 2010-03-04
CN104962579A (zh) 2015-10-07
IL202568A0 (en) 2011-08-01
HUE031698T2 (en) 2017-07-28
IL202568A (en) 2015-02-26
CN103382487A (zh) 2013-11-06
WO2008151440A4 (en) 2009-02-26
EP2155880A1 (en) 2010-02-24
US20110008837A1 (en) 2011-01-13
WO2008151440A1 (en) 2008-12-18
NZ581944A (en) 2012-03-30
RU2013137208A (ru) 2015-02-20

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Application publication date: 20101215