MX2009013665A - Produccion modificada de glicoproteinas en plantas. - Google Patents

Produccion modificada de glicoproteinas en plantas.

Info

Publication number
MX2009013665A
MX2009013665A MX2009013665A MX2009013665A MX2009013665A MX 2009013665 A MX2009013665 A MX 2009013665A MX 2009013665 A MX2009013665 A MX 2009013665A MX 2009013665 A MX2009013665 A MX 2009013665A MX 2009013665 A MX2009013665 A MX 2009013665A
Authority
MX
Mexico
Prior art keywords
plant
nucleotide sequence
protein
interest
plants
Prior art date
Application number
MX2009013665A
Other languages
English (en)
Inventor
Marc-Andre D Aoust
Estelle Marquet-Blouin
Muriel Bardor
Carole Burel
Loic Faye
Patrice Lerouge
Louis-Philippe Vezina
Veronique Gomord
Stephanie Aquin
Christophe Rihouey
Thomas Paccalet
Christophe Sourrouille
Original Assignee
Medicago Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medicago Inc filed Critical Medicago Inc
Publication of MX2009013665A publication Critical patent/MX2009013665A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Abstract

Se proporciona un método para sintetizar una proteína de interés con un perfil modificado de N-glicosilación dentro de una planta, una porción de una planta, o una célula vegetal. El método comprende co-expresar dentro de una planta una secuencia de nucleótido que codifica una primera secuencia de nucleótido que codifica una proteína híbrida (GNT1-GalT) que comprende un dominio de CTS de Nacetilgiucosaminil transferasa (GNT1) fusionado con un dominio catalítico de beta-1,4galactosiltransfera sa (GalT), la primera secuencia de nucleótido se encuentra operativamente ligada con una primera región reguladora activa en la planta, y una segunda secuencia de nucleótido para codificar la proteína de interés, la segunda secuencia de nucleótido se encuentra operativamente ligada con una segunda región reguladora que es activa en la planta. La primera y segunda secuencia de nucleótido se co-expresan para sintetizar una proteína de interés que comprende glicanos con el perfil modificado de N-glicosilación dentro de la planta, la porción de la planta, o la célula vegetal.
MX2009013665A 2007-06-15 2008-06-13 Produccion modificada de glicoproteinas en plantas. MX2009013665A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94434407P 2007-06-15 2007-06-15
PCT/CA2008/001139 WO2008151440A1 (en) 2007-06-15 2008-06-13 Modifying glycoprotein production in plants

Publications (1)

Publication Number Publication Date
MX2009013665A true MX2009013665A (es) 2010-06-02

Family

ID=40129187

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009013665A MX2009013665A (es) 2007-06-15 2008-06-13 Produccion modificada de glicoproteinas en plantas.

Country Status (24)

Country Link
US (1) US20110008837A1 (es)
EP (1) EP2155880B1 (es)
JP (1) JP5783720B2 (es)
KR (2) KR101385050B1 (es)
CN (3) CN103382487A (es)
AU (1) AU2008261527C1 (es)
BR (1) BRPI0812538B1 (es)
CA (2) CA2700180C (es)
DK (1) DK2155880T3 (es)
EG (1) EG26600A (es)
ES (1) ES2624776T3 (es)
HU (1) HUE031698T2 (es)
IL (1) IL202568A (es)
MA (1) MA31516B1 (es)
MX (1) MX2009013665A (es)
NZ (2) NZ598417A (es)
PL (1) PL2155880T3 (es)
PT (1) PT2155880T (es)
RU (2) RU2499053C2 (es)
SG (1) SG185249A1 (es)
SI (1) SI2155880T1 (es)
TN (1) TN2009000512A1 (es)
WO (1) WO2008151440A1 (es)
ZA (1) ZA201000207B (es)

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CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
CN103122354B (zh) 2007-11-27 2018-03-23 麦迪卡格公司 表达血凝素之转基因植物中生产的重组流感病毒样颗粒(vlp)
CA2730171C (en) 2008-07-08 2016-10-11 Medicago Inc. Soluble recombinant influenza antigens
CN102165062B (zh) 2008-07-18 2017-09-08 麦迪卡格公司 新的流感病毒免疫表位
JP5871796B2 (ja) 2009-06-24 2016-03-01 メディカゴ インコーポレイテッド ヘマグルチニンを含むキメラインフルエンザウイルス様粒子
JP5551780B2 (ja) 2009-09-22 2014-07-16 メディカゴ インコーポレイテッド 植物由来のタンパク質を調製する方法
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
BR112013031564B1 (pt) * 2011-06-07 2021-11-03 Ibio, Inc Métodos para geração e expressão de um polipeptídio e sistema de expressão
US9181532B2 (en) 2011-07-29 2015-11-10 Icon Genetics Gmbh Production of galactosylated N-glycans in plants
US11155581B2 (en) 2011-09-30 2021-10-26 Medicago Inc. Increasing virus-like particle yield in plants
KR20130125337A (ko) * 2012-05-08 2013-11-18 경상대학교산학협력단 고-만노오스형 n-글라이칸을 생산하는 식물체 및 이를 이용한 고-만노오스형 n-글라이칸의 생산방법
CN112592389A (zh) 2013-03-28 2021-04-02 莫迪卡戈公司 植物中流感样病毒颗粒的产生
ES2787042T3 (es) 2014-07-11 2020-10-14 Medicago Inc Modificación de la producción de proteínas en plantas
EP3221455B1 (en) 2014-11-20 2020-08-12 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
WO2018098572A1 (en) 2016-12-01 2018-06-07 Plantform Corporation Transgenic plant with reduced fucosyltransferase and xylosyltransferase activity
WO2019049111A1 (en) * 2017-09-11 2019-03-14 R. J. Reynolds Tobacco Company METHODS AND COMPOSITIONS FOR INCREASING THE EXPRESSION OF GENES OF INTEREST IN A PLANT BY CO-EXPRESSION WITH P21
US20240057540A1 (en) * 2021-01-15 2024-02-22 Denka Company Limited Method for Determining Conditions for Cultivation, and Method for Producing Desired Protein or Desired Peptide

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Also Published As

Publication number Publication date
CA2700180C (en) 2013-01-29
DK2155880T3 (en) 2016-12-05
NZ581944A (en) 2012-03-30
AU2008261527B8 (en) 2014-04-24
MA31516B1 (fr) 2010-07-01
WO2008151440A8 (en) 2010-03-04
EG26600A (en) 2014-03-23
PL2155880T3 (pl) 2017-06-30
SI2155880T1 (sl) 2017-03-31
US20110008837A1 (en) 2011-01-13
IL202568A0 (en) 2011-08-01
KR20100037602A (ko) 2010-04-09
CN103382487A (zh) 2013-11-06
AU2008261527A8 (en) 2010-01-21
JP5783720B2 (ja) 2015-09-24
WO2008151440A1 (en) 2008-12-18
EP2155880A1 (en) 2010-02-24
KR101385050B1 (ko) 2014-05-13
TN2009000512A1 (en) 2011-03-31
CN101918559A (zh) 2010-12-15
BRPI0812538A2 (pt) 2014-09-30
ZA201000207B (en) 2013-09-25
JP2010531136A (ja) 2010-09-24
CN104962579A (zh) 2015-10-07
HUE031698T2 (en) 2017-07-28
EP2155880B1 (en) 2016-08-10
EP2155880A4 (en) 2010-08-04
RU2013137208A (ru) 2015-02-20
ES2624776T3 (es) 2017-07-17
AU2008261527B2 (en) 2014-04-10
CA2700180A1 (en) 2008-12-18
KR20130075787A (ko) 2013-07-05
BRPI0812538B1 (pt) 2021-03-16
IL202568A (en) 2015-02-26
WO2008151440A4 (en) 2009-02-26
SG185249A1 (en) 2012-11-29
CA2795379A1 (en) 2008-12-18
RU2010101024A (ru) 2011-07-20
PT2155880T (pt) 2016-11-18
AU2008261527A1 (en) 2008-12-18
AU2008261527C1 (en) 2014-09-04
RU2499053C2 (ru) 2013-11-20
NZ598417A (en) 2013-06-28

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