CN101848994A - 有效建立诱导的多能干细胞的方法 - Google Patents

有效建立诱导的多能干细胞的方法 Download PDF

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CN101848994A
CN101848994A CN200980100065A CN200980100065A CN101848994A CN 101848994 A CN101848994 A CN 101848994A CN 200980100065 A CN200980100065 A CN 200980100065A CN 200980100065 A CN200980100065 A CN 200980100065A CN 101848994 A CN101848994 A CN 101848994A
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cells
oct3
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山中伸弥
吉田善纪
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Kyoto University NUC
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CN200980100065A 2008-07-30 2009-07-30 有效建立诱导的多能干细胞的方法 Pending CN101848994A (zh)

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US14117708P 2008-12-29 2008-12-29
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US20393108P 2008-12-30 2008-12-30
US61/203931 2008-12-30
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104894060A (zh) * 2014-03-03 2015-09-09 中国科学院上海生命科学研究院 诱导体细胞转分化为神经干细胞的方法及其应用
CN106459909A (zh) * 2014-01-13 2017-02-22 利昂贝拉尔中心 生产诱导多能细胞的方法
US11230696B2 (en) 2015-07-29 2022-01-25 Hadasit Medical Research Services And Development Ltd. Large scale production of retinal pigment epithelial cells

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010042490A1 (en) * 2008-10-06 2010-04-15 Boston Medical Center Corporation A single lentiviral vector system for induced pluripotent (ips) stem cells derivation
US10328103B2 (en) 2009-01-03 2019-06-25 Ray C. Wasielewski Medical treatment composition comprising mammalian dental pulp stem cells
US8470308B2 (en) * 2009-01-03 2013-06-25 Ray C. Wasielewski Enhanced medical implant comprising disrupted tooth pulp and tooth particles
JP5637354B2 (ja) * 2009-03-30 2014-12-10 独立行政法人産業技術総合研究所 精製転写因子の調製法と細胞導入技術
AU2010279913B2 (en) 2009-08-07 2016-04-28 Kyoto University Method of efficiently establishing induced pluripotent stem cells
EP2470664A4 (en) 2009-08-27 2013-01-16 Synaptic Res Llc NEW PROTEIN RELEASE SYSTEM FOR GENERATING INDUCED PLURIPOTENTAL STEM CELLS OR TISSUE-SPECIFIC CELLS
WO2011032166A2 (en) * 2009-09-14 2011-03-17 The Johns Hopkins University Reprogramming blood cells to pluripotent and multipotent stem cells
US20120276070A1 (en) * 2009-11-17 2012-11-01 Vitro Diagnositics, Inc. Induced Pluripotent Stem Cells and Related Methods
CA2802249A1 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
WO2011160128A2 (en) * 2010-06-18 2011-12-22 Cellular Dynamics International, Inc. Cardiomyocyte medium with dialyzed serum
JP6029137B2 (ja) 2010-12-03 2016-11-24 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
KR20200113286A (ko) 2010-12-22 2020-10-06 페이트 세러퓨틱스, 인코포레이티드 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼
EP3608423A1 (en) 2011-07-25 2020-02-12 Kyoto University Method for screening induced pluripotent stem cells
US20140227780A1 (en) * 2011-10-03 2014-08-14 Nissan Chemical Industries, Ltd. Method for producing megakaryocytes and/or platelets from pluripotent stem cells
JP6162604B2 (ja) 2011-10-21 2017-07-12 国立大学法人京都大学 層流による多能性維持単一分散細胞培養法
JP5999658B2 (ja) 2011-11-25 2016-09-28 国立大学法人京都大学 多能性幹細胞の培養方法
KR101930718B1 (ko) 2012-03-28 2018-12-19 가부시키가이샤 쿠오리맨 앤 코. 불사화 줄기세포 및 그 생산물을 유효 성분으로 하는 의약 조성물 및 의약 제제
JP6381442B2 (ja) 2012-05-23 2018-08-29 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
JPWO2013183718A1 (ja) * 2012-06-06 2016-02-01 国立大学法人京都大学 スクリーニング方法、タンパク質の不安定性及び／又は安定性を誘導する物質、及び、タンパク質の活性評価
EP2948776B8 (en) 2013-01-25 2020-02-26 Xcell Biosciences, Inc. Methods, compositions, kits, and systems for selective enrichment of target cells
EP2955223B1 (en) 2013-02-08 2019-12-18 Kyoto University Production methods for megakaryocytes and platelets
EP2966163B1 (en) 2013-03-06 2018-01-17 Kyoto University Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells
US9790468B2 (en) 2013-03-15 2017-10-17 Avita Iinternational Ltd. Multifunctional immature dental pulp stem cells and therapeutic applications
WO2014148646A1 (ja) 2013-03-21 2014-09-25 国立大学法人京都大学 神経分化誘導用の多能性幹細胞
EP2980207B1 (en) 2013-03-25 2018-12-05 Foundation for Biomedical Research and Innovation at Kobe Cell sorting method
EP2985344B1 (en) 2013-04-12 2018-07-18 Kyoto University Method for inducing alveolar epithelium progenitor cells
US9822342B2 (en) 2013-05-14 2017-11-21 Kyoto University Method of efficiently inducing cardiomyocytes
JP6378183B2 (ja) 2013-08-07 2018-08-22 国立大学法人京都大学 膵ホルモン産生細胞の製造法
JP6558580B2 (ja) 2013-09-05 2019-08-14 国立大学法人京都大学 新規ドーパミン産生神経前駆細胞の誘導方法
JP6635505B2 (ja) 2013-11-01 2020-01-29 国立大学法人京都大学 新規軟骨細胞誘導方法
CN106102754B (zh) * 2014-01-24 2020-04-17 石匠株式会社 癌治疗用医药组合物以及将该组合物作为有效成分的癌治疗用医药制剂
KR102460549B1 (ko) * 2014-03-04 2022-10-28 페이트 세러퓨틱스, 인코포레이티드 개선된 재프로그래밍 방법 및 세포 배양 플랫폼
EP3170897B1 (en) 2014-07-18 2020-05-13 Kyoto University Method for inducing t cells for immunocytotherapy from pluripotent stem cells
KR20250007036A (ko) 2015-10-16 2025-01-13 페이트 세러퓨틱스, 인코포레이티드 기저 상태 다능성의 유도 및 유지를 위한 플랫폼
WO2017183736A1 (ja) 2016-04-22 2017-10-26 国立大学法人京都大学 ドーパミン産生神経前駆細胞の製造方法
WO2017223199A1 (en) * 2016-06-22 2017-12-28 Xcell Biosciences, Inc. Methods for increasing cell culture transfection efficiency and cellular reprogramming
EP4595951A3 (en) 2016-11-10 2025-08-27 ViaCyte, Inc. Pdx1 pancreatic endoderm cells in cell delivery devices
JP7285015B2 (ja) 2018-07-19 2023-06-01 国立大学法人京都大学 多能性幹細胞由来の板状軟骨およびその製造方法
WO2020022261A1 (ja) 2018-07-23 2020-01-30 国立大学法人京都大学 新規腎前駆細胞マーカーおよびそれを利用した腎前駆細胞の濃縮方法
EP3900787A4 (en) 2018-12-21 2022-02-23 Kyoto University LUBRICIN-LOCALIZED CARTILE-LIKE TISSUE, METHOD OF MANUFACTURE THEREOF AND COMPOSITION THEREOF FOR TREATMENT OF ARTICULATE-CARTILAGE DEFECTS
WO2020227677A1 (en) * 2019-05-09 2020-11-12 Figene, Llc Fibroblast generated patient-specific vaccines
EP3969571A4 (en) 2019-05-14 2023-05-24 Aleph Farms Ltd. AGGREGATES OF PLURIPOTENT CELLS AND THEIR USE
WO2021117886A1 (ja) 2019-12-12 2021-06-17 国立大学法人千葉大学 巨核球および血小板を含む凍結乾燥製剤
JP7429294B2 (ja) 2020-07-13 2024-02-07 国立大学法人京都大学 骨格筋前駆細胞及びその精製方法、筋原性疾患を治療するための組成物、並びに骨格筋前駆細胞を含む細胞群の製造方法
EP4180514A4 (en) 2020-07-20 2024-09-04 Aichi Medical University COMPOSITION FOR UNDIFFERENTIATED MAINTENANCE CULTURE OF PLURIPOTENT CELLS, MEDIUM FOR UNDIFFERENTIATED MAINTENANCE CULTURE OF PLURIPOTENT CELLS, METHOD FOR MAINTENANCE CULTURE IN UNDIFFERENTIATED STATE OF PLURIPOTENT CELLS, AND METHOD FOR PRODUCING PLURIPOTENT CELLS
EP4477746A1 (en) 2022-02-09 2024-12-18 Sumitomo Pharma Co., Ltd. Method for assessing differentiation potential of cells in culture broth in differentiation of pluripotent stem cells into neural cells of midbrain floor plate region
WO2024248017A1 (ja) 2023-05-29 2024-12-05 国立研究開発法人理化学研究所 心筋細胞を成熟させる方法及び成熟した心筋細胞の製造方法

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999020741A1 (en) 1997-10-23 1999-04-29 Geron Corporation Methods and materials for the growth of primate-derived primordial stem cells
US20020090722A1 (en) * 2000-06-15 2002-07-11 Tanja Dominko Pluripotent mammalian cells
PT1572984E (pt) 2002-12-16 2016-06-03 Technion Res & Dev Foundation Sistema de cultura para células estaminais embrionárias humanas sem células alimentadoras e sem xeno
WO2006019366A1 (en) 2003-03-28 2006-02-23 Wisconsin Alumni Research Foundation Physiochemical culture conditions for embryonic stem cells
GB2432835B (en) 2004-09-08 2009-04-08 Wisconsin Alumni Res Found Culturing human embryonic stem cells
US20060275899A1 (en) 2004-12-30 2006-12-07 Stemlifeline, Inc. Methods and compositions relating to embryonic stem cell lines
ZA200804673B (en) 2005-12-13 2009-11-25 Univ Kyoto Nuclear reprogramming factor
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
CN101605885B (zh) 2007-01-04 2013-03-27 卡尔·特吕格瓦松 能够使多能干细胞增殖的组合物及方法
WO2008118820A2 (en) * 2007-03-23 2008-10-02 Wisconsin Alumni Research Foundation Somatic cell reprogramming
US20120282229A1 (en) 2007-08-01 2012-11-08 Christian Kannemeier Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state
WO2009073523A2 (en) 2007-11-29 2009-06-11 Children's Hospital Of Orange County De-differentiation of human cells
WO2009111087A1 (en) 2008-03-07 2009-09-11 Worcester Polytechnic Institute Novel use of basic fibroblast growth factor in the de-differentiation of animal connective tissue cells
US20100279404A1 (en) 2008-05-02 2010-11-04 Shinya Yamanaka Method of nuclear reprogramming
WO2009142717A2 (en) 2008-05-19 2009-11-26 President And Fellows Of Harvard College Methods and products for dedifferentiation of cells
JP5340265B2 (ja) 2008-06-27 2013-11-13 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
EP2333050A4 (en) 2008-09-04 2013-07-03 Riken IPS CELLS FROM B-CELLS AND ITS APPLICATION
US8945922B2 (en) 2008-09-08 2015-02-03 Riken Generating a mature NKT cell from a reprogrammed somatic cell with a T-cell antigen receptor α-chain region rearranged to uniform Va-Ja in a NKT-cell specific way
CN101492676B (zh) * 2008-09-16 2011-02-16 中国科学院广州生物医药与健康研究院 用脑膜细胞生成诱导的多能性干细胞的方法及其用途
AU2010279913B2 (en) 2009-08-07 2016-04-28 Kyoto University Method of efficiently establishing induced pluripotent stem cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRIAN KEITH, ET AL: "Hypoxia-Inducible Factors, Stem Cells, and Cancer", 《CELL》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106459909A (zh) * 2014-01-13 2017-02-22 利昂贝拉尔中心 生产诱导多能细胞的方法
CN104894060A (zh) * 2014-03-03 2015-09-09 中国科学院上海生命科学研究院 诱导体细胞转分化为神经干细胞的方法及其应用
CN104894060B (zh) * 2014-03-03 2018-11-06 中国科学院上海生命科学研究院 诱导体细胞转分化为神经干细胞的方法及其应用
US11230696B2 (en) 2015-07-29 2022-01-25 Hadasit Medical Research Services And Development Ltd. Large scale production of retinal pigment epithelial cells

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CA2697621C (en) 2017-01-17
WO2010013845A1 (en) 2010-02-04
JP2011529329A (ja) 2011-12-08
EP2307539A1 (en) 2011-04-13
CA2697621A1 (en) 2010-02-04
KR20110036523A (ko) 2011-04-07
JP5562231B2 (ja) 2014-07-30
US20110039338A1 (en) 2011-02-17
EP2307539B1 (en) 2014-11-19
KR101685209B1 (ko) 2016-12-09
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US9528092B2 (en) 2016-12-27

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