JP5562231B2 - 効率的な人工多能性幹細胞の樹立方法 - Google Patents
効率的な人工多能性幹細胞の樹立方法 Download PDFInfo
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- JP5562231B2 JP5562231B2 JP2010506476A JP2010506476A JP5562231B2 JP 5562231 B2 JP5562231 B2 JP 5562231B2 JP 2010506476 A JP2010506476 A JP 2010506476A JP 2010506476 A JP2010506476 A JP 2010506476A JP 5562231 B2 JP5562231 B2 JP 5562231B2
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/867—Retroviral vectors
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/604—Klf-4
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/606—Transcription factors c-Myc
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
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- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Transplantation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8484208P | 2008-07-30 | 2008-07-30 | |
| US61/084,842 | 2008-07-30 | ||
| US14117708P | 2008-12-29 | 2008-12-29 | |
| US61/141,177 | 2008-12-29 | ||
| US20393108P | 2008-12-30 | 2008-12-30 | |
| US61/203,931 | 2008-12-30 | ||
| PCT/JP2009/063906 WO2010013845A1 (en) | 2008-07-30 | 2009-07-30 | Method of efficiently establishing induced pluripotent stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011529329A JP2011529329A (ja) | 2011-12-08 |
| JP2011529329A5 JP2011529329A5 (enExample) | 2012-08-23 |
| JP5562231B2 true JP5562231B2 (ja) | 2014-07-30 |
Family
ID=41610532
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010506476A Active JP5562231B2 (ja) | 2008-07-30 | 2009-07-30 | 効率的な人工多能性幹細胞の樹立方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9528092B2 (enExample) |
| EP (1) | EP2307539B1 (enExample) |
| JP (1) | JP5562231B2 (enExample) |
| KR (1) | KR101685209B1 (enExample) |
| CN (2) | CN107012123A (enExample) |
| CA (1) | CA2697621C (enExample) |
| WO (1) | WO2010013845A1 (enExample) |
Families Citing this family (49)
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| WO2010042490A1 (en) * | 2008-10-06 | 2010-04-15 | Boston Medical Center Corporation | A single lentiviral vector system for induced pluripotent (ips) stem cells derivation |
| US10328103B2 (en) | 2009-01-03 | 2019-06-25 | Ray C. Wasielewski | Medical treatment composition comprising mammalian dental pulp stem cells |
| US8470308B2 (en) * | 2009-01-03 | 2013-06-25 | Ray C. Wasielewski | Enhanced medical implant comprising disrupted tooth pulp and tooth particles |
| JP5637354B2 (ja) * | 2009-03-30 | 2014-12-10 | 独立行政法人産業技術総合研究所 | 精製転写因子の調製法と細胞導入技術 |
| CN102625837B (zh) | 2009-08-07 | 2015-01-14 | 国立大学法人京都大学 | 有效建立诱导的多能干细胞的方法 |
| JP2013503198A (ja) | 2009-08-27 | 2013-01-31 | シナプティック リサーチ,リミテッド ライアビリティ カンパニー | 人工多能性幹(iPS)細胞または組織特異的細胞を誘導するための新規タンパク質送達系 |
| CN102803476A (zh) * | 2009-09-14 | 2012-11-28 | 程临钊 | 血细胞重编程产生多潜能干细胞和多功能干细胞 |
| WO2011062963A2 (en) * | 2009-11-17 | 2011-05-26 | Vitro Diagnositics, Inc. | Induced puripotent stem cells and related methods |
| CN103003416B (zh) | 2010-06-15 | 2016-11-16 | 细胞动力学国际有限公司 | 从小体积的外周血产生诱导性多潜能干细胞 |
| US9365826B2 (en) * | 2010-06-18 | 2016-06-14 | Cellular Dynamics International, Inc. | Cardiomyocyte medium with dialyzed serum |
| US9506039B2 (en) | 2010-12-03 | 2016-11-29 | Kyoto University | Efficient method for establishing induced pluripotent stem cells |
| KR20140063501A (ko) | 2010-12-22 | 2014-05-27 | 페이트 세러퓨틱스, 인코포레이티드 | 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼 |
| WO2013014929A1 (en) | 2011-07-25 | 2013-01-31 | Kyoto University | Method for screening induced pluripotent stem cells |
| RU2014117642A (ru) * | 2011-10-03 | 2015-11-10 | Ниссан Кемикал Индастриз, Лтд. | Способ получения мегакариоцитов и/или тромбоцитов из плюрипотентных стволовых клеток |
| WO2013058403A1 (ja) | 2011-10-21 | 2013-04-25 | 国立大学法人京都大学 | 層流による多能性維持単一分散細胞培養法 |
| US20140329317A1 (en) | 2011-11-25 | 2014-11-06 | Kyoto University | Method for culturing pluripotent stem cell |
| EP2832851B1 (en) * | 2012-03-28 | 2019-04-17 | Quarrymen & Co. Inc. | Immortalized stem cells and medicinal composition and medicinal preparation comprising product thereof as active ingredient |
| CN104471060B (zh) | 2012-05-23 | 2021-11-05 | 国立大学法人京都大学 | 用于建立诱导性多能干细胞的高效方法 |
| WO2013183718A1 (ja) * | 2012-06-06 | 2013-12-12 | 国立大学法人京都大学 | スクリーニング方法、タンパク質の不安定性及び/又は安定性を誘導する物質、及び、タンパク質の活性評価 |
| WO2014117021A2 (en) | 2013-01-25 | 2014-07-31 | Xcell Biosciences, Inc. | Methods, compositions, kits, and systems for selective enrichment of target cells |
| WO2014123242A1 (ja) | 2013-02-08 | 2014-08-14 | 国立大学法人京都大学 | 巨核球及び血小板の製造方法 |
| WO2014136581A1 (ja) | 2013-03-06 | 2014-09-12 | 国立大学法人京都大学 | 多能性幹細胞の培養システム及び多能性幹細胞の継代方法 |
| US9790468B2 (en) | 2013-03-15 | 2017-10-17 | Avita Iinternational Ltd. | Multifunctional immature dental pulp stem cells and therapeutic applications |
| WO2014148646A1 (ja) | 2013-03-21 | 2014-09-25 | 国立大学法人京都大学 | 神経分化誘導用の多能性幹細胞 |
| US10072242B2 (en) | 2013-03-25 | 2018-09-11 | Foundation For Biomedical Research And Innovation At Kobe | Cell sorting method |
| EP2985344B1 (en) | 2013-04-12 | 2018-07-18 | Kyoto University | Method for inducing alveolar epithelium progenitor cells |
| EP2998391B1 (en) | 2013-05-14 | 2020-02-19 | Kyoto University | Efficient myocardial cell induction method |
| JP6378183B2 (ja) | 2013-08-07 | 2018-08-22 | 国立大学法人京都大学 | 膵ホルモン産生細胞の製造法 |
| ES2721440T3 (es) | 2013-09-05 | 2019-07-31 | Univ Kyoto | Nuevo método para inducir células precursoras neurales productoras de dopamina |
| EP3064577B1 (en) | 2013-11-01 | 2020-09-09 | Kyoto University | Novel chondrocyte induction method |
| FR3016372B1 (fr) * | 2014-01-13 | 2018-01-19 | Centre Leon Berard | Procede de preparation de cellules pluripotentes induites |
| KR102297734B1 (ko) * | 2014-01-24 | 2021-09-03 | 가부시키가이샤 쿠오리맨 앤 코. | 암 치료용 의약 조성물 및 그 조성물을 유효 성분으로 하는 암 치료용 의약 제제 |
| CN104894060B (zh) * | 2014-03-03 | 2018-11-06 | 中国科学院上海生命科学研究院 | 诱导体细胞转分化为神经干细胞的方法及其应用 |
| ES2966757T3 (es) * | 2014-03-04 | 2024-04-24 | Fate Therapeutics Inc | Métodos de reprogramación mejorados y plataformas de cultivo celular |
| SG10201900323SA (en) | 2014-07-18 | 2019-02-27 | Univ Kyoto | Method for inducing t cells for cell-based immunotherapy from pluripotent stem cells |
| US11230696B2 (en) | 2015-07-29 | 2022-01-25 | Hadasit Medical Research Services And Development Ltd. | Large scale production of retinal pigment epithelial cells |
| WO2017066634A1 (en) | 2015-10-16 | 2017-04-20 | Fate Therapeutics, Inc. | Platform for the induction & maintenance of ground state pluripotency |
| KR102312123B1 (ko) | 2016-04-22 | 2021-10-13 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 도파민 생산 신경전구세포의 제조방법 |
| WO2017223199A1 (en) * | 2016-06-22 | 2017-12-28 | Xcell Biosciences, Inc. | Methods for increasing cell culture transfection efficiency and cellular reprogramming |
| CA3042495A1 (en) | 2016-11-10 | 2018-05-17 | Viacyte, Inc. | Pdx1 pancreatic endoderm cells in cell delivery devices and methods thereof |
| EP3824912A4 (en) | 2018-07-19 | 2022-04-20 | Kyoto University | PLATEFORM CARTILAGE DERIVED FROM PLURIPOTENTS STEM CELLS AND METHODS FOR PRODUCTION OF PLATEFORM CARTILAGE |
| US20210332329A1 (en) | 2018-07-23 | 2021-10-28 | Kyoto University | Novel renal progenitor cell marker and method for concentrating renal progenitor cells using same |
| WO2020130147A1 (ja) | 2018-12-21 | 2020-06-25 | 国立大学法人京都大学 | ルブリシン局在軟骨様組織、その製造方法及びそれを含む関節軟骨損傷治療用組成物 |
| US20220313734A1 (en) * | 2019-05-09 | 2022-10-06 | Figene, Llc | Fibroblast generated patient-specific vaccines |
| AU2020273728A1 (en) | 2019-05-14 | 2021-11-18 | Aleph Farms Ltd. | Pluripotent cell aggregates and use thereof |
| WO2021117886A1 (ja) | 2019-12-12 | 2021-06-17 | 国立大学法人千葉大学 | 巨核球および血小板を含む凍結乾燥製剤 |
| WO2022014604A1 (ja) | 2020-07-13 | 2022-01-20 | 国立大学法人京都大学 | 骨格筋前駆細胞及びその精製方法、筋原性疾患を治療するための組成物、並びに骨格筋前駆細胞を含む細胞群の製造方法 |
| CN116134130A (zh) | 2020-07-20 | 2023-05-16 | 学校法人爱知医科大学 | 多能细胞的未分化维持培养用组合物、多能细胞的未分化维持培养用培养基、多能细胞的未分化状态下的维持培养方法、和多能细胞的制造方法 |
| EP4477746A1 (en) | 2022-02-09 | 2024-12-18 | Sumitomo Pharma Co., Ltd. | Method for assessing differentiation potential of cells in culture broth in differentiation of pluripotent stem cells into neural cells of midbrain floor plate region |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU729377B2 (en) | 1997-10-23 | 2001-02-01 | Asterias Biotherapeutics, Inc. | Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture |
| AU6842001A (en) * | 2000-06-15 | 2001-12-24 | Tanja Dominko | Pluripotent mammalian cells |
| KR101156745B1 (ko) | 2002-12-16 | 2012-06-15 | 테크니온 리서치 앤드 디벨롭먼트 파운데이션 리미티드 | 지지세포 비함유, 이종 비함유의 인간 배아줄기세포의 조제방법 및 이들을 사용하여 조제되는 줄기세포 배양물 |
| WO2006019366A1 (en) | 2003-03-28 | 2006-02-23 | Wisconsin Alumni Research Foundation | Physiochemical culture conditions for embryonic stem cells |
| BRPI0514641A (pt) | 2004-09-08 | 2008-06-17 | Wisconsin Alumni Res Found | cultivação das células tronco embriÈnica humana |
| WO2006073911A1 (en) * | 2004-12-30 | 2006-07-13 | Stemlifeline, Inc. | Methods and compositions relating to embryonic stem cell lines |
| WO2007069666A1 (ja) * | 2005-12-13 | 2007-06-21 | Kyoto University | 核初期化因子 |
| US8278104B2 (en) * | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
| US20090227032A1 (en) * | 2005-12-13 | 2009-09-10 | Kyoto University | Nuclear reprogramming factor and induced pluripotent stem cells |
| US8722405B2 (en) | 2007-01-04 | 2014-05-13 | Karl Tryggvason | Composition and method for enabling proliferation of pluripotent stem cells |
| SG193652A1 (en) | 2007-03-23 | 2013-10-30 | Wisconsin Alumni Res Found | Somatic cell reprogramming |
| WO2009032456A2 (en) | 2007-08-01 | 2009-03-12 | Primegen Biotech Llc | Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state |
| EP2227540A4 (en) | 2007-11-29 | 2011-11-02 | Children S Hospital Of Orange County | DIFFERENTIATION OF HUMAN CELLS |
| US9290740B2 (en) | 2008-03-07 | 2016-03-22 | Worcester Polytechnic Institute | Use of basic fibroblast growth factor in the de-differentiation of animal connective tissue cells |
| CA2695522C (en) * | 2008-05-02 | 2019-01-15 | Kyoto University | Method of nuclear reprogramming |
| WO2009142717A2 (en) * | 2008-05-19 | 2009-11-26 | President And Fellows Of Harvard College | Methods and products for dedifferentiation of cells |
| CN101835890B (zh) * | 2008-06-27 | 2013-07-24 | 国立大学法人京都大学 | 有效建立诱导的多能干细胞的方法 |
| JPWO2010027062A1 (ja) * | 2008-09-04 | 2012-02-02 | 独立行政法人理化学研究所 | B細胞由来iPS細胞およびその用途 |
| EP2336303B1 (en) | 2008-09-08 | 2015-07-15 | Riken | NKT CELL-DERIVED iPS CELLS AND NKT CELLS DERIVED THEREFROM |
| CN101492676B (zh) * | 2008-09-16 | 2011-02-16 | 中国科学院广州生物医药与健康研究院 | 用脑膜细胞生成诱导的多能性干细胞的方法及其用途 |
| CN102625837B (zh) * | 2009-08-07 | 2015-01-14 | 国立大学法人京都大学 | 有效建立诱导的多能干细胞的方法 |
-
2009
- 2009-07-30 CN CN201611216942.9A patent/CN107012123A/zh active Pending
- 2009-07-30 US US12/672,222 patent/US9528092B2/en active Active
- 2009-07-30 CN CN200980100065A patent/CN101848994A/zh active Pending
- 2009-07-30 KR KR1020107002641A patent/KR101685209B1/ko active Active
- 2009-07-30 JP JP2010506476A patent/JP5562231B2/ja active Active
- 2009-07-30 EP EP09803075.2A patent/EP2307539B1/en active Active
- 2009-07-30 WO PCT/JP2009/063906 patent/WO2010013845A1/en not_active Ceased
- 2009-07-30 CA CA2697621A patent/CA2697621C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2307539A1 (en) | 2011-04-13 |
| KR101685209B1 (ko) | 2016-12-09 |
| JP2011529329A (ja) | 2011-12-08 |
| WO2010013845A1 (en) | 2010-02-04 |
| CN107012123A (zh) | 2017-08-04 |
| EP2307539B1 (en) | 2014-11-19 |
| CN101848994A (zh) | 2010-09-29 |
| US9528092B2 (en) | 2016-12-27 |
| US20110039338A1 (en) | 2011-02-17 |
| CA2697621C (en) | 2017-01-17 |
| KR20110036523A (ko) | 2011-04-07 |
| CA2697621A1 (en) | 2010-02-04 |
| EP2307539A4 (en) | 2012-10-24 |
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