JP5562231B2 - 効率的な人工多能性幹細胞の樹立方法 - Google Patents

効率的な人工多能性幹細胞の樹立方法 Download PDF

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JP5562231B2
JP5562231B2 JP2010506476A JP2010506476A JP5562231B2 JP 5562231 B2 JP5562231 B2 JP 5562231B2 JP 2010506476 A JP2010506476 A JP 2010506476A JP 2010506476 A JP2010506476 A JP 2010506476A JP 5562231 B2 JP5562231 B2 JP 5562231B2
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伸弥 山中
善紀 吉田
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Kyoto University NUC
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2510/00—Genetically modified cells

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JP2010506476A 2008-07-30 2009-07-30 効率的な人工多能性幹細胞の樹立方法 Active JP5562231B2 (ja)

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Application Number Priority Date Filing Date Title
US8484208P 2008-07-30 2008-07-30
US61/084,842 2008-07-30
US14117708P 2008-12-29 2008-12-29
US61/141,177 2008-12-29
US20393108P 2008-12-30 2008-12-30
US61/203,931 2008-12-30
PCT/JP2009/063906 WO2010013845A1 (en) 2008-07-30 2009-07-30 Method of efficiently establishing induced pluripotent stem cells

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JP2011529329A5 JP2011529329A5 (enExample) 2012-08-23
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EP (1) EP2307539B1 (enExample)
JP (1) JP5562231B2 (enExample)
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CN (2) CN101848994A (enExample)
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Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010042490A1 (en) * 2008-10-06 2010-04-15 Boston Medical Center Corporation A single lentiviral vector system for induced pluripotent (ips) stem cells derivation
US8470308B2 (en) * 2009-01-03 2013-06-25 Ray C. Wasielewski Enhanced medical implant comprising disrupted tooth pulp and tooth particles
US10328103B2 (en) 2009-01-03 2019-06-25 Ray C. Wasielewski Medical treatment composition comprising mammalian dental pulp stem cells
JP5637354B2 (ja) * 2009-03-30 2014-12-10 独立行政法人産業技術総合研究所 精製転写因子の調製法と細胞導入技術
WO2011016588A1 (en) 2009-08-07 2011-02-10 Kyoto University Method of efficiently establishing induced pluripotent stem cells
CN102612562A (zh) 2009-08-27 2012-07-25 色奈普提科研究有限公司 一种新的用于生成诱导性多能干(iPS)细胞或组织特异性细胞的蛋白质递送系统
CN102803476A (zh) * 2009-09-14 2012-11-28 程临钊 血细胞重编程产生多潜能干细胞和多功能干细胞
US20120276070A1 (en) * 2009-11-17 2012-11-01 Vitro Diagnositics, Inc. Induced Pluripotent Stem Cells and Related Methods
WO2011159684A2 (en) * 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
EP2582792B1 (en) * 2010-06-18 2018-10-31 FUJIFILM Cellular Dynamics, Inc. Cardiomyocyte medium with dialyzed serum
US9506039B2 (en) 2010-12-03 2016-11-29 Kyoto University Efficient method for establishing induced pluripotent stem cells
AU2011349446C1 (en) 2010-12-22 2016-01-21 Fate Therapauetics, Inc. Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs
CN103703130A (zh) 2011-07-25 2014-04-02 国立大学法人京都大学 筛选诱导的多能干细胞的方法
EP2765187B1 (en) * 2011-10-03 2018-08-08 Nissan Chemical Industries, Ltd. Method for producing megakaryocytes and/or platelets from pluripotent stem cells
WO2013058403A1 (ja) 2011-10-21 2013-04-25 国立大学法人京都大学 層流による多能性維持単一分散細胞培養法
US20140329317A1 (en) 2011-11-25 2014-11-06 Kyoto University Method for culturing pluripotent stem cell
IN2014DN08862A (enExample) * 2012-03-28 2015-05-22 Quarrymen Corp
AU2013264708B2 (en) 2012-05-23 2019-03-07 Kyoto University Highly efficient method for establishing artificial pluripotent stem cell
JPWO2013183718A1 (ja) * 2012-06-06 2016-02-01 国立大学法人京都大学 スクリーニング方法、タンパク質の不安定性及び／又は安定性を誘導する物質、及び、タンパク質の活性評価
WO2014117021A2 (en) 2013-01-25 2014-07-31 Xcell Biosciences, Inc. Methods, compositions, kits, and systems for selective enrichment of target cells
US20160002599A1 (en) 2013-02-08 2016-01-07 Kyoto University Production methods for megakaryocytes and platelets
EP2966163B1 (en) 2013-03-06 2018-01-17 Kyoto University Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells
US9790468B2 (en) 2013-03-15 2017-10-17 Avita Iinternational Ltd. Multifunctional immature dental pulp stem cells and therapeutic applications
US10519421B2 (en) 2013-03-21 2019-12-31 Kyoto University Induction of motor neurons from pluripotent stem cells
US10072242B2 (en) 2013-03-25 2018-09-11 Foundation For Biomedical Research And Innovation At Kobe Cell sorting method
CA2909230C (en) 2013-04-12 2021-06-15 Kyoto University Method for inducing alveolar epithelial progenitor cells
JP6429280B2 (ja) 2013-05-14 2018-11-28 国立大学法人京都大学 効率的な心筋細胞の誘導方法
WO2015020113A1 (ja) 2013-08-07 2015-02-12 国立大学法人京都大学 膵ホルモン産生細胞の製造法
CN105849255A (zh) 2013-09-05 2016-08-10 国立大学法人京都大学 新的产多巴胺神经前体细胞诱导方法
KR102219743B1 (ko) 2013-11-01 2021-02-23 고쿠리츠 다이가쿠 호진 교토 다이가쿠 신규 연골 세포 유도 방법
FR3016372B1 (fr) * 2014-01-13 2018-01-19 Centre Leon Berard Procede de preparation de cellules pluripotentes induites
CN106102754B (zh) * 2014-01-24 2020-04-17 石匠株式会社 癌治疗用医药组合物以及将该组合物作为有效成分的癌治疗用医药制剂
CN104894060B (zh) * 2014-03-03 2018-11-06 中国科学院上海生命科学研究院 诱导体细胞转分化为神经干细胞的方法及其应用
SG11201606934SA (en) * 2014-03-04 2016-09-29 Fate Therapeutics Inc Improved reprogramming methods and cell culture platforms
SG10201900323SA (en) 2014-07-18 2019-02-27 Univ Kyoto Method for inducing t cells for cell-based immunotherapy from pluripotent stem cells
JP7055095B2 (ja) * 2015-07-29 2022-04-15 ハダシット メディカル リサーチ サービシーズ アンド ディベロップメント リミテッド 網膜色素上皮細胞の大規模生産
CA3001917A1 (en) 2015-10-16 2017-04-20 Fate Therapeutics, Inc. Platform for the induction & maintenance of ground state pluripotency
WO2017183736A1 (ja) 2016-04-22 2017-10-26 国立大学法人京都大学 ドーパミン産生神経前駆細胞の製造方法
WO2017223199A1 (en) * 2016-06-22 2017-12-28 Xcell Biosciences, Inc. Methods for increasing cell culture transfection efficiency and cellular reprogramming
JP7088564B2 (ja) 2016-11-10 2022-06-21 ヴィアサイト，インコーポレイテッド 細胞送達装置におけるｐｄｘ１膵臓内胚葉細胞及びその方法
CA3103823A1 (en) * 2018-06-14 2019-12-19 Figene, Llc Use of fibroblasts and/or modified fibroblasts for three dimensional tissue printing
EP3824912A4 (en) 2018-07-19 2022-04-20 Kyoto University PLATEFORM CARTILAGE DERIVED FROM PLURIPOTENTS STEM CELLS AND METHODS FOR PRODUCTION OF PLATEFORM CARTILAGE
JP7357369B2 (ja) 2018-07-23 2023-10-06 国立大学法人京都大学 新規腎前駆細胞マーカーおよびそれを利用した腎前駆細胞の濃縮方法
JP7224001B2 (ja) 2018-12-21 2023-02-17 国立大学法人京都大学 ルブリシン局在軟骨様組織、その製造方法及びそれを含む関節軟骨損傷治療用組成物
WO2020227677A1 (en) * 2019-05-09 2020-11-12 Figene, Llc Fibroblast generated patient-specific vaccines
WO2020230138A1 (en) 2019-05-14 2020-11-19 Aleph Farms Ltd. Pluripotent cell aggregates and use thereof
US20230030814A1 (en) 2019-12-12 2023-02-02 National University Corporation Chiba University Freeze-Dried Preparations Comprising Megakaryocytes and Platelets
US20230256024A1 (en) 2020-07-13 2023-08-17 Kyoto University Skeletal muscle precursor cells and method for purifying same, composition for treating myogenic diseases, and method for producing cell group containing skeletal muscle precursor cells
EP4180514A4 (en) 2020-07-20 2024-09-04 Aichi Medical University COMPOSITION FOR UNDIFFERENTIATED MAINTENANCE CULTURE OF PLURIPOTENT CELLS, MEDIUM FOR UNDIFFERENTIATED MAINTENANCE CULTURE OF PLURIPOTENT CELLS, METHOD FOR MAINTENANCE CULTURE IN UNDIFFERENTIATED STATE OF PLURIPOTENT CELLS, AND METHOD FOR PRODUCING PLURIPOTENT CELLS
EP4477746A4 (en) 2022-02-09 2026-02-18 Racthera Co Ltd METHOD FOR EVALUATING THE DIFFERENTIATION POTENTIAL OF CELLS IN A CULTURE BROTH IN THE DIFFERENCE OF MULTIPOTENTED STEM CELLS INTO NEURAL CELLS OF THE MENSCEPHAL FLOOR PLAQUE REGION
EP4722345A1 (en) 2023-05-29 2026-04-08 Riken Method for maturing cardiomyocytes, and method for producing matured cardiomyocytes

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3880795B2 (ja) 1997-10-23 2007-02-14 ジェロン・コーポレーション フィーダー細胞を含まない培養物中で、霊長類由来始原幹細胞を増殖させるための方法
WO2001096532A2 (en) * 2000-06-15 2001-12-20 Tanja Dominko Method of generating pluripotent mammalian cells by fusion of a cytoplast fragment with a karyoplast
EP1572984B8 (en) 2002-12-16 2016-06-01 Technion Research & Development Foundation Ltd. Feeder-free, xeno-free culture system for human embryonic stem cells
US20040224401A1 (en) 2003-03-28 2004-11-11 Ludwig Tenneille E. Physiochemical culture conditions for embryonic stem cells
DK3196296T3 (en) 2004-09-08 2019-02-04 Wisconsin Alumini Res Foundation Cultivation of human embryonic stem cells
US20060275899A1 (en) 2004-12-30 2006-12-07 Stemlifeline, Inc. Methods and compositions relating to embryonic stem cell lines
US20090227032A1 (en) * 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
CN101864392B (zh) * 2005-12-13 2016-03-23 国立大学法人京都大学 核重新编程因子
US8278104B2 (en) * 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
CN101605885B (zh) 2007-01-04 2013-03-27 卡尔·特吕格瓦松 能够使多能干细胞增殖的组合物及方法
EP3399025B1 (en) * 2007-03-23 2025-05-07 Wisconsin Alumni Research Foundation Somatic cell reprogramming
US20120282229A1 (en) 2007-08-01 2012-11-08 Christian Kannemeier Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state
WO2009073523A2 (en) 2007-11-29 2009-06-11 Children's Hospital Of Orange County De-differentiation of human cells
US9290740B2 (en) 2008-03-07 2016-03-22 Worcester Polytechnic Institute Use of basic fibroblast growth factor in the de-differentiation of animal connective tissue cells
CN101855350B (zh) * 2008-05-02 2014-12-31 国立大学法人京都大学 核重编程序方法
WO2009142717A2 (en) * 2008-05-19 2009-11-26 President And Fellows Of Harvard College Methods and products for dedifferentiation of cells
KR101606943B1 (ko) * 2008-06-27 2016-03-28 고쿠리츠 다이가쿠 호진 교토 다이가쿠 유도된 다능성 줄기 세포의 효율적인 확립 방법
US20110231944A1 (en) * 2008-09-04 2011-09-22 Riken B cell-derived ips cells and application thereof
JP5652783B2 (ja) 2008-09-08 2015-01-14 独立行政法人理化学研究所 ＮＫＴ細胞由来ｉＰＳ細胞およびそれ由来のＮＫＴ細胞
CN101492676B (zh) * 2008-09-16 2011-02-16 中国科学院广州生物医药与健康研究院 用脑膜细胞生成诱导的多能性干细胞的方法及其用途
WO2011016588A1 (en) * 2009-08-07 2011-02-10 Kyoto University Method of efficiently establishing induced pluripotent stem cells

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CA2697621A1 (en) 2010-02-04
KR101685209B1 (ko) 2016-12-09
KR20110036523A (ko) 2011-04-07
US9528092B2 (en) 2016-12-27
US20110039338A1 (en) 2011-02-17
EP2307539A1 (en) 2011-04-13
JP2011529329A (ja) 2011-12-08
CN101848994A (zh) 2010-09-29
EP2307539B1 (en) 2014-11-19
WO2010013845A1 (en) 2010-02-04
CA2697621C (en) 2017-01-17
EP2307539A4 (en) 2012-10-24
CN107012123A (zh) 2017-08-04

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