KR101685209B1 - 유도된 다능성 줄기 세포의 효율적인 확립 방법 - Google Patents

유도된 다능성 줄기 세포의 효율적인 확립 방법 Download PDF

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KR101685209B1
KR101685209B1 KR1020107002641A KR20107002641A KR101685209B1 KR 101685209 B1 KR101685209 B1 KR 101685209B1 KR 1020107002641 A KR1020107002641 A KR 1020107002641A KR 20107002641 A KR20107002641 A KR 20107002641A KR 101685209 B1 KR101685209 B1 KR 101685209B1
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신야 야마나카
요시노리 요시다
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고쿠리츠 다이가쿠 호진 교토 다이가쿠
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KR1020107002641A 2008-07-30 2009-07-30 유도된 다능성 줄기 세포의 효율적인 확립 방법 Active KR101685209B1 (ko)

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US8484208P 2008-07-30 2008-07-30
US61/084,842 2008-07-30
US14117708P 2008-12-29 2008-12-29
US61/141,177 2008-12-29
US20393108P 2008-12-30 2008-12-30
US61/203,931 2008-12-30
PCT/JP2009/063906 WO2010013845A1 (en) 2008-07-30 2009-07-30 Method of efficiently establishing induced pluripotent stem cells

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CN (2) CN101848994A (enExample)
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Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010042490A1 (en) * 2008-10-06 2010-04-15 Boston Medical Center Corporation A single lentiviral vector system for induced pluripotent (ips) stem cells derivation
US10328103B2 (en) 2009-01-03 2019-06-25 Ray C. Wasielewski Medical treatment composition comprising mammalian dental pulp stem cells
US8470308B2 (en) * 2009-01-03 2013-06-25 Ray C. Wasielewski Enhanced medical implant comprising disrupted tooth pulp and tooth particles
JP5637354B2 (ja) * 2009-03-30 2014-12-10 独立行政法人産業技術総合研究所 精製転写因子の調製法と細胞導入技術
WO2011016588A1 (en) 2009-08-07 2011-02-10 Kyoto University Method of efficiently establishing induced pluripotent stem cells
US8420352B2 (en) 2009-08-27 2013-04-16 Synaptic Research, Llc Protein delivery system to generate pluripotent stem (iPS) cells or tissue specific cells
US20120301438A1 (en) * 2009-09-14 2012-11-29 The Johns Hopkins University Reprogramming Blood Cells to Pluripotent and Multipotent Stem Cells
WO2011062963A2 (en) * 2009-11-17 2011-05-26 Vitro Diagnositics, Inc. Induced puripotent stem cells and related methods
US8691574B2 (en) * 2010-06-15 2014-04-08 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
KR101861171B1 (ko) * 2010-06-18 2018-05-31 후지필름 셀룰러 다이내믹스, 인코포레이티드 투석된 혈청이 있는 심근세포 배지
EP2647699B1 (en) 2010-12-03 2020-04-01 Kyoto University Efficient method for establishing induced pluripotent stem cells
CN106893692B (zh) 2010-12-22 2021-11-26 菲特治疗公司 用于单细胞分选与增强ipsc重新编程的细胞培养平台
CN107988381B (zh) 2011-07-25 2021-05-28 国立大学法人京都大学 筛选诱导的多能干细胞的方法
US20140227780A1 (en) * 2011-10-03 2014-08-14 Nissan Chemical Industries, Ltd. Method for producing megakaryocytes and/or platelets from pluripotent stem cells
JP6162604B2 (ja) 2011-10-21 2017-07-12 国立大学法人京都大学 層流による多能性維持単一分散細胞培養法
WO2013077423A1 (ja) 2011-11-25 2013-05-30 国立大学法人京都大学 多能性幹細胞の培養方法
RU2619221C2 (ru) * 2012-03-28 2017-05-12 Кварримен Корпорейшн Иммортализованная стволовая клетка и лекарственная композиция и лекарственный препарат, содержащие ее в качестве активного ингредиента
EP2853592B1 (en) 2012-05-23 2019-02-20 Kyoto University Highly efficient method for establishing artificial pluripotent stem cell
WO2013183718A1 (ja) * 2012-06-06 2013-12-12 国立大学法人京都大学 スクリーニング方法、タンパク質の不安定性及び/又は安定性を誘導する物質、及び、タンパク質の活性評価
CA2895791A1 (en) 2013-01-25 2014-07-31 Xcell Biosciences, Inc. Methods, compositions, kits, and systems for selective enrichment of target cells
JP6495658B2 (ja) 2013-02-08 2019-04-03 国立大学法人京都大学 巨核球及び血小板の製造方法
WO2014136581A1 (ja) 2013-03-06 2014-09-12 国立大学法人京都大学 多能性幹細胞の培養システム及び多能性幹細胞の継代方法
US9790468B2 (en) 2013-03-15 2017-10-17 Avita Iinternational Ltd. Multifunctional immature dental pulp stem cells and therapeutic applications
EP2977449B1 (en) 2013-03-21 2020-02-26 Kyoto University Pluripotent stem cell for neuronal differentiation induction
WO2014157257A1 (ja) 2013-03-25 2014-10-02 公益財団法人先端医療振興財団 細胞の選別方法
JP6461787B2 (ja) 2013-04-12 2019-01-30 国立大学法人京都大学 肺胞上皮前駆細胞の誘導方法
JP6429280B2 (ja) 2013-05-14 2018-11-28 国立大学法人京都大学 効率的な心筋細胞の誘導方法
US9796962B2 (en) 2013-08-07 2017-10-24 Kyoto University Method for generating pancreatic hormone-producing cells
SG11201601720RA (en) 2013-09-05 2016-04-28 Univ Kyoto New method for inducing dopamine-producing neural precursor cells
JP6635505B2 (ja) 2013-11-01 2020-01-29 国立大学法人京都大学 新規軟骨細胞誘導方法
FR3016372B1 (fr) * 2014-01-13 2018-01-19 Centre Leon Berard Procede de preparation de cellules pluripotentes induites
EP3093023B1 (en) * 2014-01-24 2020-11-04 Quarrymen & Co. Inc. Pharmaceutical composition for cancer treatment
CN104894060B (zh) * 2014-03-03 2018-11-06 中国科学院上海生命科学研究院 诱导体细胞转分化为神经干细胞的方法及其应用
SG10201807292YA (en) * 2014-03-04 2018-09-27 Fate Therapeutics Inc Improved reprogramming methods and cell culture platforms
US12241089B2 (en) 2014-07-18 2025-03-04 Kyoto University Method for inducing t cells for cell-based immunotherapy from pluripotent stem cells
US11230696B2 (en) 2015-07-29 2022-01-25 Hadasit Medical Research Services And Development Ltd. Large scale production of retinal pigment epithelial cells
AU2016338680B2 (en) 2015-10-16 2022-11-17 Fate Therapeutics, Inc. Platform for the induction and maintenance of ground state pluripotency
US11898163B2 (en) 2016-04-22 2024-02-13 Kyoto University Method for producing dopaminergic neuron progenitor cell
WO2017223199A1 (en) * 2016-06-22 2017-12-28 Xcell Biosciences, Inc. Methods for increasing cell culture transfection efficiency and cellular reprogramming
JP7088564B2 (ja) 2016-11-10 2022-06-21 ヴィアサイト,インコーポレイテッド 細胞送達装置におけるpdx1膵臓内胚葉細胞及びその方法
EP3824912A4 (en) 2018-07-19 2022-04-20 Kyoto University PLATEFORM CARTILAGE DERIVED FROM PLURIPOTENTS STEM CELLS AND METHODS FOR PRODUCTION OF PLATEFORM CARTILAGE
US20210332329A1 (en) 2018-07-23 2021-10-28 Kyoto University Novel renal progenitor cell marker and method for concentrating renal progenitor cells using same
EP3900787A4 (en) 2018-12-21 2022-02-23 Kyoto University LUBRICIN-LOCALIZED CARTILE-LIKE TISSUE, METHOD OF MANUFACTURE THEREOF AND COMPOSITION THEREOF FOR TREATMENT OF ARTICULATE-CARTILAGE DEFECTS
CA3139836A1 (en) * 2019-05-09 2020-11-12 Figene, Llc Fibroblast generated patient-specific vaccines
EP3969571A4 (en) 2019-05-14 2023-05-24 Aleph Farms Ltd. AGGREGATES OF PLURIPOTENT CELLS AND THEIR USE
EP4074321A4 (en) 2019-12-12 2024-01-03 National University Corporation Chiba University Freeze-dried preparation containing megakaryocytes and platelets
EP4180516A4 (en) 2020-07-13 2024-01-17 Kyoto University SKELETON MUSCLE PRECURSOR CELLS AND METHOD FOR PURIFICATION THEREOF, COMPOSITION FOR THE TREATMENT OF MYOGENIC DISEASES AND METHOD FOR PRODUCING SKELETON MUSCLE PRECURSOR CELLS CONTAINING CELL GROUPS
JPWO2022019152A1 (enExample) 2020-07-20 2022-01-27
JPWO2023153464A1 (enExample) 2022-02-09 2023-08-17

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006073911A1 (en) * 2004-12-30 2006-07-13 Stemlifeline, Inc. Methods and compositions relating to embryonic stem cell lines

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1025204A4 (en) 1997-10-23 2001-02-28 Geron Corp Methods and materials for the growth of primate-derived primordial stem cells
WO2001096532A2 (en) * 2000-06-15 2001-12-20 Tanja Dominko Method of generating pluripotent mammalian cells by fusion of a cytoplast fragment with a karyoplast
EP2457999B1 (en) 2002-12-16 2018-10-17 Technion Research & Development Foundation Ltd. Culture medium for pluripotent stem cells
WO2006019366A1 (en) 2003-03-28 2006-02-23 Wisconsin Alumni Research Foundation Physiochemical culture conditions for embryonic stem cells
US7442548B2 (en) 2004-09-08 2008-10-28 Wisconsin Alumni Research Foundation Culturing human embryonic stem cells in medium containing pipecholic acid and gamma amino butyric acid
KR101420740B1 (ko) 2005-12-13 2014-07-17 교또 다이가꾸 핵초기화 인자
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
DK2137295T3 (en) 2007-01-04 2018-06-25 Karl Tryggvason Use of a composition and method for enabling proliferation of pluripotent stem cells
EP2137296A2 (en) * 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Somatic cell reprogramming
US20120282229A1 (en) 2007-08-01 2012-11-08 Christian Kannemeier Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state
US20110033931A1 (en) 2007-11-29 2011-02-10 Children's Hospital Of Orange County De-differentiation of human cells
US9290740B2 (en) 2008-03-07 2016-03-22 Worcester Polytechnic Institute Use of basic fibroblast growth factor in the de-differentiation of animal connective tissue cells
ES2722198T3 (es) 2008-05-02 2019-08-08 Univ Kyoto Método de reprogramación nuclear
WO2009142717A2 (en) * 2008-05-19 2009-11-26 President And Fellows Of Harvard College Methods and products for dedifferentiation of cells
KR101606943B1 (ko) 2008-06-27 2016-03-28 고쿠리츠 다이가쿠 호진 교토 다이가쿠 유도된 다능성 줄기 세포의 효율적인 확립 방법
US20110231944A1 (en) 2008-09-04 2011-09-22 Riken B cell-derived ips cells and application thereof
ES2549155T3 (es) 2008-09-08 2015-10-23 Riken Células iPS obtenidas a partir de células NKT y células NKT obtenidas a partir de las mismas
CN101492676B (zh) * 2008-09-16 2011-02-16 中国科学院广州生物医药与健康研究院 用脑膜细胞生成诱导的多能性干细胞的方法及其用途
WO2011016588A1 (en) 2009-08-07 2011-02-10 Kyoto University Method of efficiently establishing induced pluripotent stem cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006073911A1 (en) * 2004-12-30 2006-07-13 Stemlifeline, Inc. Methods and compositions relating to embryonic stem cell lines

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Cell. 131(5):861-872(2007.11.30.)*
Mol. Pharmacol. 69(4):1304-1310(2006.04.)*

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KR20110036523A (ko) 2011-04-07
CN101848994A (zh) 2010-09-29
EP2307539A1 (en) 2011-04-13
US20110039338A1 (en) 2011-02-17
CA2697621C (en) 2017-01-17
US9528092B2 (en) 2016-12-27
WO2010013845A1 (en) 2010-02-04
EP2307539A4 (en) 2012-10-24
JP2011529329A (ja) 2011-12-08
CA2697621A1 (en) 2010-02-04
EP2307539B1 (en) 2014-11-19
CN107012123A (zh) 2017-08-04
JP5562231B2 (ja) 2014-07-30

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