CN101768616B - 含稳定聚合酶的反应化合物的干组合物 - Google Patents

含稳定聚合酶的反应化合物的干组合物 Download PDF

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Publication number
CN101768616B
CN101768616B CN200910263837.4A CN200910263837A CN101768616B CN 101768616 B CN101768616 B CN 101768616B CN 200910263837 A CN200910263837 A CN 200910263837A CN 101768616 B CN101768616 B CN 101768616B
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liquid mixture
dry composition
polymerase
aptamer
pcr
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CN101768616A (zh
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T·哈斯林格
T·梅茨勒
A·佩塞尼
H·索贝克
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F Hoffmann La Roche AG
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200910263837.4A 2008-12-19 2009-12-17 含稳定聚合酶的反应化合物的干组合物 Active CN101768616B (zh)

Applications Claiming Priority (2)

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EP08022082.5 2008-12-19
EP08022082 2008-12-19

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CN101768616A CN101768616A (zh) 2010-07-07
CN101768616B true CN101768616B (zh) 2014-07-09

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US (3) US8652811B2 (OSRAM)
EP (1) EP2202302B1 (OSRAM)
JP (2) JP5731113B2 (OSRAM)
CN (1) CN101768616B (OSRAM)
CA (1) CA2688174C (OSRAM)
ES (1) ES2394754T3 (OSRAM)

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CN103849548A (zh) 2012-12-03 2014-06-11 三星电子株式会社 用于扩增核酸的试剂容器及其制备方法、存储试剂的方法和用于核酸分析的微流体系统
EP3249054A1 (en) 2012-12-20 2017-11-29 Biomatrica, INC. Formulations and methods for stabilizing pcr reagents
JP5904153B2 (ja) * 2013-03-29 2016-04-13 ソニー株式会社 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ
CA2912307C (en) 2013-06-11 2022-01-11 Biocartis Nv Biomolecule drying process for long-term storage
US10036061B2 (en) 2013-12-20 2018-07-31 Roche Molecular Systems, Inc. Oligonucleotide inhibitor of DNA polymerases
EP3154338B1 (en) 2014-06-10 2020-01-29 Biomatrica, INC. Stabilization of thrombocytes at ambient temperatures
EP3015553A1 (de) * 2014-10-30 2016-05-04 Biotecon Diagnostics GmbH Stabilisierte Reaktionsmischung
KR102787387B1 (ko) 2015-12-08 2025-03-26 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
GB201716963D0 (en) * 2017-10-16 2017-11-29 Biofortuna Ltd Compositions and methods for use in stabilising nucleic acid amplification assays
CN109486824B (zh) * 2018-11-20 2021-11-16 安徽省昂普拓迈生物科技有限责任公司 一种特异性结合Taq酶的核酸适配体及其筛选方法、应用
CN111961709A (zh) * 2020-01-21 2020-11-20 上海酷乐生物科技有限公司 一种用于口腔拭子直接荧光pcr扩增的试剂和试剂盒
WO2024106507A1 (ja) * 2022-11-18 2024-05-23 日油株式会社 核酸増幅用の乾燥組成物およびそれを用いた核酸増幅法
CN115851889B (zh) * 2022-11-30 2025-12-05 北京全式金生物技术股份有限公司 松三糖和可逆结合因子Oligo在提升荧光定量PCR全预混体系的保存稳定性中的应用

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Also Published As

Publication number Publication date
CA2688174C (en) 2018-08-07
US9163286B2 (en) 2015-10-20
JP2015091261A (ja) 2015-05-14
US20100159529A1 (en) 2010-06-24
JP5731113B2 (ja) 2015-06-10
US20140162267A1 (en) 2014-06-12
US20160237473A1 (en) 2016-08-18
EP2202302B1 (en) 2012-09-12
ES2394754T3 (es) 2013-02-05
US9631233B2 (en) 2017-04-25
CN101768616A (zh) 2010-07-07
JP2010142229A (ja) 2010-07-01
US8652811B2 (en) 2014-02-18
CA2688174A1 (en) 2010-06-19
EP2202302A1 (en) 2010-06-30

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