ES2394754T3 - Composición seca de compuestos de reacción con una polimerasa estabilizada - Google Patents
Composición seca de compuestos de reacción con una polimerasa estabilizada Download PDFInfo
- Publication number
- ES2394754T3 ES2394754T3 ES09015595T ES09015595T ES2394754T3 ES 2394754 T3 ES2394754 T3 ES 2394754T3 ES 09015595 T ES09015595 T ES 09015595T ES 09015595 T ES09015595 T ES 09015595T ES 2394754 T3 ES2394754 T3 ES 2394754T3
- Authority
- ES
- Spain
- Prior art keywords
- reaction compounds
- dry composition
- aptamer
- liquid mixture
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08022082 | 2008-12-19 | ||
| EP08022082 | 2008-12-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2394754T3 true ES2394754T3 (es) | 2013-02-05 |
Family
ID=40510578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09015595T Active ES2394754T3 (es) | 2008-12-19 | 2009-12-17 | Composición seca de compuestos de reacción con una polimerasa estabilizada |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US8652811B2 (OSRAM) |
| EP (1) | EP2202302B1 (OSRAM) |
| JP (2) | JP5731113B2 (OSRAM) |
| CN (1) | CN101768616B (OSRAM) |
| CA (1) | CA2688174C (OSRAM) |
| ES (1) | ES2394754T3 (OSRAM) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011140489A2 (en) * | 2010-05-06 | 2011-11-10 | Ibis Biosciences, Inc. | Integrated sample preparation systems and stabilized enzyme mixtures |
| EP2598661B1 (en) | 2010-07-26 | 2017-09-27 | Biomatrica, INC. | Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures |
| CA2806670A1 (en) | 2010-07-26 | 2012-02-09 | Biomatrica, Inc. | Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures |
| EP2625284B1 (en) | 2010-10-04 | 2015-01-28 | Roche Diagniostics GmbH | Method for cell lysis in a rt-pcr reaction buffer |
| CN103124797B (zh) | 2010-10-04 | 2015-04-22 | 霍夫曼-拉罗奇有限公司 | 用于在相同反应容器内的细胞裂解和pcr的方法 |
| JP2013005796A (ja) * | 2011-05-26 | 2013-01-10 | Arkray Inc | 乾燥試薬、乾燥試薬キット、試薬器、乾燥試薬の製造方法 |
| EP2574931B1 (en) | 2011-09-29 | 2017-03-22 | Qiagen GmbH | Dry composition comprising a control dye |
| WO2013053855A1 (en) * | 2011-10-11 | 2013-04-18 | Qiagen Gmbh | Sample processing method and sample processing cartridge |
| WO2013068107A1 (en) | 2011-11-07 | 2013-05-16 | Qiagen Gmbh | Lysis method and lysis composition |
| EP2730653A1 (en) | 2012-11-07 | 2014-05-14 | QIAGEN GmbH | Method for lysing a fixed biological sample |
| CN103849548A (zh) | 2012-12-03 | 2014-06-11 | 三星电子株式会社 | 用于扩增核酸的试剂容器及其制备方法、存储试剂的方法和用于核酸分析的微流体系统 |
| EP3249054A1 (en) | 2012-12-20 | 2017-11-29 | Biomatrica, INC. | Formulations and methods for stabilizing pcr reagents |
| JP5904153B2 (ja) * | 2013-03-29 | 2016-04-13 | ソニー株式会社 | 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ |
| CA2912307C (en) | 2013-06-11 | 2022-01-11 | Biocartis Nv | Biomolecule drying process for long-term storage |
| US10036061B2 (en) * | 2013-12-20 | 2018-07-31 | Roche Molecular Systems, Inc. | Oligonucleotide inhibitor of DNA polymerases |
| JP6661554B2 (ja) | 2014-06-10 | 2020-03-11 | バイオマトリカ，インク． | 周囲温度における血小板の安定化 |
| EP3015553A1 (de) * | 2014-10-30 | 2016-05-04 | Biotecon Diagnostics GmbH | Stabilisierte Reaktionsmischung |
| CN113588501B (zh) | 2015-12-08 | 2024-12-17 | 生物马特里卡公司 | 降低红细胞沉降速率 |
| GB201716963D0 (en) * | 2017-10-16 | 2017-11-29 | Biofortuna Ltd | Compositions and methods for use in stabilising nucleic acid amplification assays |
| CN109486824B (zh) * | 2018-11-20 | 2021-11-16 | 安徽省昂普拓迈生物科技有限责任公司 | 一种特异性结合Taq酶的核酸适配体及其筛选方法、应用 |
| CN111961709A (zh) * | 2020-01-21 | 2020-11-20 | 上海酷乐生物科技有限公司 | 一种用于口腔拭子直接荧光pcr扩增的试剂和试剂盒 |
| EP4621069A1 (en) * | 2022-11-18 | 2025-09-24 | NOF Corporation | Dry composition for nucleic acid amplification uses, and nucleic acid amplification method using same |
| CN115851889B (zh) * | 2022-11-30 | 2025-12-05 | 北京全式金生物技术股份有限公司 | 松三糖和可逆结合因子Oligo在提升荧光定量PCR全预混体系的保存稳定性中的应用 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3300474A (en) | 1964-02-12 | 1967-01-24 | Pharmacia Ab | Sucrose ether copolymerizates |
| US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| GB8826429D0 (en) | 1988-11-11 | 1988-12-14 | Univ Leeds Ind Service Ltd | Enzyme stabilisation systems |
| GB8903593D0 (en) | 1989-02-16 | 1989-04-05 | Pafra Ltd | Storage of materials |
| ES2110444T3 (es) | 1990-06-11 | 1998-02-16 | Nexstar Pharmaceuticals Inc | Ligandos de acidos nucleicos. |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5200399A (en) | 1990-09-14 | 1993-04-06 | Boyce Thompson Institute For Plant Research, Inc. | Method of protecting biological materials from destructive reactions in the dry state |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5565318A (en) | 1994-09-02 | 1996-10-15 | Pharmacia Biotech, Inc. | Room temperature stable reagent semi-spheres |
| US5593824A (en) | 1994-09-02 | 1997-01-14 | Pharmacia Biotech, Inc. | Biological reagent spheres |
| ES2434258T3 (es) | 1996-06-04 | 2013-12-16 | University Of Utah Research Foundation | Aparato para llevar a cabo la PCR y monitorización de la reacción en tiempo real durante ciclos de temperatura |
| ATE428801T1 (de) | 1996-06-04 | 2009-05-15 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| EP1002875A1 (en) * | 1998-11-17 | 2000-05-24 | Mira Diagnostica GmbH | An oligonucleotide inhibitor of thermostable polymerase |
| PT1232502E (pt) | 1999-11-17 | 2006-05-31 | Roche Diagnostics Gmbh | Particulas de vidro magneticas, metodo para a sua preparacao e suas utilizacoes |
| ES2294287T5 (es) | 2002-01-08 | 2015-10-27 | F. Hoffmann-La Roche Ag | Empleo de un material de sílice en una reacción de amplificación |
| US7407747B2 (en) * | 2002-10-15 | 2008-08-05 | Applera Corporation | Method for drying dye-terminator sequencing reagents |
| US7820808B2 (en) * | 2003-04-01 | 2010-10-26 | Eragen Biosciences, Inc. | Polymerase inhibitor and method of using same |
| EP1502958A1 (en) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | New detection format for hot start real time polymerase chain reaction |
| EP1674569A1 (en) | 2003-09-30 | 2006-06-28 | Riken | Support having enzyme immobilized thereon, print, reagent kit, process for producing the support, method of preserving enzyme and method of revitalizing enzyme |
| US20060099567A1 (en) * | 2004-04-08 | 2006-05-11 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
| WO2006131391A1 (en) * | 2005-06-10 | 2006-12-14 | Epigenomics Ag | Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients |
| WO2007106580A2 (en) * | 2006-03-15 | 2007-09-20 | Micronics, Inc. | Rapid magnetic flow assays |
| WO2007137291A1 (en) * | 2006-05-23 | 2007-11-29 | Molecular Detection, Inc. | Ambient temperature stable kits for molecular diagnostics |
| CN104774924B (zh) | 2006-09-18 | 2021-04-13 | 通用电气医疗集团生物科学公司 | 用玻璃覆盖的生物学试剂的制备 |
| DE102006056790B3 (de) * | 2006-12-01 | 2008-01-17 | IfP Privates Institut für Produktqualität GmbH | Laboreinmalartikel für Analyse und Diagnosik |
| WO2008155529A1 (en) * | 2007-06-16 | 2008-12-24 | Enigma Diagnostics Limited | Compositions |
-
2009
- 2009-12-11 CA CA2688174A patent/CA2688174C/en active Active
- 2009-12-17 ES ES09015595T patent/ES2394754T3/es active Active
- 2009-12-17 JP JP2009286615A patent/JP5731113B2/ja active Active
- 2009-12-17 EP EP09015595A patent/EP2202302B1/en active Active
- 2009-12-17 CN CN200910263837.4A patent/CN101768616B/zh active Active
- 2009-12-18 US US12/641,747 patent/US8652811B2/en active Active
-
2014
- 2014-01-31 US US14/169,993 patent/US9163286B2/en active Active
-
2015
- 2015-01-09 JP JP2015003548A patent/JP2015091261A/ja active Pending
- 2015-09-16 US US14/855,915 patent/US9631233B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US9631233B2 (en) | 2017-04-25 |
| US20140162267A1 (en) | 2014-06-12 |
| CN101768616A (zh) | 2010-07-07 |
| US9163286B2 (en) | 2015-10-20 |
| JP5731113B2 (ja) | 2015-06-10 |
| US8652811B2 (en) | 2014-02-18 |
| US20100159529A1 (en) | 2010-06-24 |
| EP2202302A1 (en) | 2010-06-30 |
| CA2688174A1 (en) | 2010-06-19 |
| CN101768616B (zh) | 2014-07-09 |
| EP2202302B1 (en) | 2012-09-12 |
| CA2688174C (en) | 2018-08-07 |
| JP2015091261A (ja) | 2015-05-14 |
| JP2010142229A (ja) | 2010-07-01 |
| US20160237473A1 (en) | 2016-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2394754T3 (es) | Composición seca de compuestos de reacción con una polimerasa estabilizada | |
| ES3022638T3 (en) | Recombinase polymerase amplification reagents | |
| AU2010258715B2 (en) | Rehydratable matrices for dry storage of TAQ polymerase in a microfluidic device | |
| US8911938B2 (en) | Reaction chamber having pre-stored reagents | |
| KR20190096989A (ko) | 핵산 샘플을 프로세싱하는 방법 | |
| JP2009538146A (ja) | 室温で安定な分子診断用キット | |
| US10525471B2 (en) | Target analysis chip and target analysis method | |
| KR20240158392A (ko) | 서열 특이적 오류가 감소된 조작된 중합효소 | |
| CA2761059C (en) | A dried and stabilized ready-to-use composition containing nucleic acid polymerization enzymes for molecular biology applications | |
| ES2674522T3 (es) | Composición liofilizada | |
| US10550423B2 (en) | Macromolecule delivery to nanowells | |
| ES2717538T3 (es) | Mezclas de reacción estables | |
| JP5608998B2 (ja) | 保存安定性に優れた核酸増幅検出試薬キット | |
| WO2017184028A1 (en) | Stabilized mixture of reagents for molecular diagnostics | |
| US20180291413A1 (en) | Devices and methods for producing nucleic acids and proteins | |
| JP5608997B2 (ja) | 保存安定性に優れた核酸増幅検出試薬キット | |
| Pavankumar et al. | Direct fluorescent imaging of translocation and unwinding by individual DNA helicases | |
| WO2024085132A1 (ja) | 生体物質処理方法及び反応検出方法並びに生体物質処理装置及び反応検出装置 | |
| JP2008079506A (ja) | ポリメラーゼ反応安定剤 |