CN101501216B - 制备重组人凝血酶的方法 - Google Patents

制备重组人凝血酶的方法 Download PDF

Info

Publication number
CN101501216B
CN101501216B CN2007800143376A CN200780014337A CN101501216B CN 101501216 B CN101501216 B CN 101501216B CN 2007800143376 A CN2007800143376 A CN 2007800143376A CN 200780014337 A CN200780014337 A CN 200780014337A CN 101501216 B CN101501216 B CN 101501216B
Authority
CN
China
Prior art keywords
human
gly
thrombin
glu
ser
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2007800143376A
Other languages
English (en)
Chinese (zh)
Other versions
CN101501216A (zh
Inventor
K·奥斯瑟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Humagene Inc
Original Assignee
Humagene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Humagene Inc filed Critical Humagene Inc
Publication of CN101501216A publication Critical patent/CN101501216A/zh
Application granted granted Critical
Publication of CN101501216B publication Critical patent/CN101501216B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Diabetes (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
CN2007800143376A 2006-03-06 2007-03-06 制备重组人凝血酶的方法 Expired - Fee Related CN101501216B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US77947406P 2006-03-06 2006-03-06
US60/779,474 2006-03-06
US84394506P 2006-09-12 2006-09-12
US60/843,945 2006-09-12
PCT/US2007/005845 WO2007103447A2 (en) 2006-03-06 2007-03-06 A method for the preparation of recombinant human thrombin

Publications (2)

Publication Number Publication Date
CN101501216A CN101501216A (zh) 2009-08-05
CN101501216B true CN101501216B (zh) 2013-10-02

Family

ID=38475538

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2007800143376A Expired - Fee Related CN101501216B (zh) 2006-03-06 2007-03-06 制备重组人凝血酶的方法

Country Status (8)

Country Link
US (2) US20100159512A1 (enExample)
EP (2) EP1996186A4 (enExample)
JP (1) JP2009528843A (enExample)
CN (1) CN101501216B (enExample)
AU (1) AU2007223880A1 (enExample)
CA (1) CA2644926A1 (enExample)
RU (1) RU2008139428A (enExample)
WO (1) WO2007103447A2 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009223115B2 (en) 2008-03-14 2015-09-24 Humanzyme, Inc. Recombinant production of authentic human proteins using human cell expression systems
EP3225252A1 (en) 2008-05-02 2017-10-04 Laboratoire Français du Fractionnement et des Biotechnologies Treatment of bleeding with low half-life fibrinogen
NZ590871A (en) * 2008-07-09 2012-07-27 Profibrix Bv Recombinant fibrinogen
FR2942232B1 (fr) * 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine de la coagulation et procedes pour sa mise en oeuvre
US20120164111A1 (en) 2009-04-14 2012-06-28 Kurt Osther Novel post-translational fibrinogen variants
PL2624859T3 (pl) * 2010-10-06 2017-09-29 Medimmune Limited Czynnik ii samodzielnie lub w skojarzeniu z dalszymi czynnikami do leczenia zaburzeń hemostazy powiązanych z koagulopatią z rozcieńczenia
JP5839810B2 (ja) 2011-03-03 2016-01-06 日本製粉株式会社 フィブリノゲンを産生するトランスジェニックカイコ
CN102690803B (zh) * 2011-03-24 2015-04-29 苏州泽璟生物制药有限公司 一种重组人凝血酶在动物细胞内的高效表达和生产方法
EP2556842A1 (en) 2011-08-11 2013-02-13 Bioftalmik, S.L. Composition in the form of film comprising fibrinogen and a fibrinogen activator and the applications thereof
HK1199908A1 (en) 2011-09-06 2015-07-24 Medimmune, Llc Methods for processing coagulation factors
WO2014115087A2 (en) * 2013-01-22 2014-07-31 Cencor Biotech Ltd. A method for the preparation of recombinant human prothrombin and fibrinogen
EP3274367A1 (en) * 2015-03-23 2018-01-31 Dana Genetic A/S Recombinant soluble human tissue factor, method of its production and uses thereof
WO2016176440A2 (en) * 2015-04-28 2016-11-03 Saint Louis University Thrombin-thrombomodulin fusion proteins as protein c activators
WO2017189943A1 (en) * 2016-04-28 2017-11-02 Saint Louis University Thrombin-thrombomodulin fusion proteins as a powerful anticoagulant
BR102017004788B1 (pt) * 2017-03-09 2021-03-23 Instituto De Biologia Molecular Do Paraná - Ibmp. Método de produção de cola de fibrina de origem recombinante
EP3618853B1 (en) * 2018-04-24 2020-09-09 Fibriant B.V. Fibrinogen gamma prime variants for use in treating an infection
JP2025519280A (ja) 2022-05-17 2025-06-25 ユリウス－マキシミリアンズ－ユニヴァーシタエト ビュルツブルク 手術創ケア用のフィブリンシーラントのための新規組換えフィブリノーゲンバリアント
CN116333094B (zh) * 2023-02-04 2024-03-29 山东多美康生物医药有限公司 一种重组人源化I型胶原蛋白α1及表达载体和应用
CN116218864B (zh) * 2023-04-10 2024-01-09 山东多美康生物医药有限公司 一种重组Ⅲ型人源化胶原蛋白α1及其表达载体和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1074438A (zh) * 1991-11-12 1993-07-21 伊莱利利公司 抗血栓剂
US5476777A (en) * 1991-12-31 1995-12-19 Zymogenetics, Inc. Methods for producing thrombin
CN1127509A (zh) * 1993-06-03 1996-07-24 阿斯特拉公司 新的肽衍生物
CN1139455A (zh) * 1993-11-12 1997-01-01 吉里德科学公司 凝血酶突变体

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US722366A (en) 1901-09-09 1903-03-10 Lars Peter Larsen Regenerative apparatus for the simultaneous warming and cooling of milk.
ATE20824T1 (de) 1981-06-25 1986-08-15 Serapharm Gmbh & Co Kg Angereichertes plasmaderivat zur unterstuetzung von wundverschluss und wundheilung.
US4442655A (en) 1981-06-25 1984-04-17 Serapharm Michael Stroetmann Fibrinogen-containing dry preparation, manufacture and use thereof
US4546082A (en) 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
US4713339A (en) 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
US4870008A (en) 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
US4613572A (en) 1983-08-15 1986-09-23 Kansas State University Research Foundation Yeast BAR1 gene plasmid
US4931373A (en) 1984-05-25 1990-06-05 Zymogenetics, Inc. Stable DNA constructs for expression of α-1 antitrypsin
EP0188555A4 (en) 1984-07-09 1988-04-18 Integrated Genetics Inc YEAST CLONING VEHICLE.
JPS6144825A (ja) 1984-08-09 1986-03-04 Unitika Ltd 止血剤
EP0243465A1 (en) 1985-10-25 1987-11-04 Vivian L. Mackay Method of using bar1 for secreting foreign proteins
US4965203A (en) 1987-01-28 1990-10-23 Warner-Lambert Company Purified thrombin preparations
US5572692A (en) 1991-12-24 1996-11-05 Intel Corporation Memory configuration decoding system having automatic row base address generation mechanism for variable memory devices with row access interleaving
US5527692A (en) 1991-12-31 1996-06-18 Zymogenetics, Inc. Methods for producing thrombin
IL102929A (en) * 1992-08-24 1996-11-14 Interpharm Lab Ltd Serum-free medium for mammalian cells
US5968817A (en) * 1993-03-15 1999-10-19 The Scripps Research Institute DNA encoding serotonin receptors
US5639940A (en) 1994-03-03 1997-06-17 Pharmaceutical Proteins Ltd. Production of fibrinogen in transgenic animals
DE19605126A1 (de) * 1996-02-12 1997-08-14 Basf Ag Thrombinmuteine als Antidot für Thrombininhibitoren
US6037457A (en) 1997-01-31 2000-03-14 The University Of North Carolina At Chapel Hill Method for recombinant fibrinogen production
US6780411B2 (en) 1998-05-01 2004-08-24 Zymogenetics, Inc. Tissue sealant compositions
AT409379B (de) * 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
CA2446185C (en) * 2001-05-07 2013-06-18 National Research Council Of Canada Enhanced production of recombinant proteins by transient transfection of suspension-growing mammalian cells
CA2452767C (en) * 2001-07-06 2013-03-19 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Genetic recombinant ecarin and process for preparing the same
KR20040032147A (ko) * 2001-07-06 2004-04-14 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 유전자 재조합 기술에 의한 인간 트롬빈 제조 방법
WO2003052059A2 (en) * 2001-12-14 2003-06-26 Eli Lilly And Company Recombinant bovine thrombin
WO2004007714A1 (ja) * 2002-07-17 2004-01-22 Asahi Kasei Pharma Corporation ヒトプロトロンビン類似体およびヒトメゾトロンビン類似体
JP3994159B2 (ja) * 2003-03-24 2007-10-17 農林水産省動物医薬品検査所長 動物由来成分無添加培養液およびそれを用いる細胞系の作出方法
GB0324044D0 (en) * 2003-10-14 2003-11-19 Astrazeneca Ab Protein
EP1676911B1 (en) * 2003-10-24 2010-04-21 Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute Novel recombinant animal cells with high protein production, method of constructing the same and method of mass protein production using the same
MX2007001294A (es) * 2004-08-17 2008-03-04 Zlb Behring Gmbh Polipeptidos modificados que dependen de vitamina k.
WO2008008862A2 (en) * 2006-07-11 2008-01-17 Invitrogen Corporation Proteome standards for mass spectrometry

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1074438A (zh) * 1991-11-12 1993-07-21 伊莱利利公司 抗血栓剂
US5476777A (en) * 1991-12-31 1995-12-19 Zymogenetics, Inc. Methods for producing thrombin
CN1127509A (zh) * 1993-06-03 1996-07-24 阿斯特拉公司 新的肽衍生物
CN1139455A (zh) * 1993-11-12 1997-01-01 吉里德科学公司 凝血酶突变体

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
第6栏第50-54段.

Also Published As

Publication number Publication date
JP2009528843A (ja) 2009-08-13
US20100159512A1 (en) 2010-06-24
EP1996186A4 (en) 2009-07-22
EP2407561A2 (en) 2012-01-18
WO2007103447A2 (en) 2007-09-13
CN101501216A (zh) 2009-08-05
CA2644926A1 (en) 2007-09-13
US20130017591A1 (en) 2013-01-17
RU2008139428A (ru) 2010-05-20
WO2007103447A3 (en) 2008-12-11
EP2407561A3 (en) 2012-05-30
WO2007103447A8 (en) 2008-05-15
AU2007223880A1 (en) 2007-09-13
EP1996186A2 (en) 2008-12-03

Similar Documents

Publication Publication Date Title
CN101501216B (zh) 制备重组人凝血酶的方法
KR101273229B1 (ko) 변형된 활성화 특성을 갖는 응고 인자 ｘ 폴리펩타이드
ES2315022T3 (es) Metodo para la produccion de fvii.
JPH09509045A (ja) トロンビン変異体
US20090169553A1 (en) Novel Protein Fusion/Tag Technology
US6171842B1 (en) Chimeric serine proteases
US20020064517A1 (en) Fibrin sealant as a transfection/transformation vehicle for gene therapy
JP5823088B2 (ja) 止血を調節する組成物および方法
JPH04505554A (ja) 可溶性トロンボモジュリン類似体
US20150337284A1 (en) Factor ix variants
AU753458B2 (en) Fibrinogen-converting enzyme hybrids
US7993893B2 (en) Haemocoagulase
KR20160103965A (ko) 인자 vii을 포함하는 융합 단백질의 분리 및 정제 방법
KR20000047835A (ko) 혈액 응고 인자 및 ｐ-셀렉틴의 ｄｎａ 작제물
EP0927764B1 (de) Chimäre Serinproteasen
WO2014115087A2 (en) A method for the preparation of recombinant human prothrombin and fibrinogen
CN120400110A (zh) 一种经修饰的重组人凝血酶及其制备方法、用途
JP2012509670A (ja) 血液凝固促進剤としてのヘビ第五因子及び使用方法
HK1151184B (en) Recombinant fibrinogen
EP1208212A1 (en) Fibrin sealant as a transfection/transformation vehicle for gene therapy
HK1151184A1 (zh) 重组纤维蛋白原
JPH01104175A (ja) 組換え精製プロテア−ゼネキシン
JPH04182436A (ja) 医薬組成物

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20131002

Termination date: 20160306