CN101455178A - Culture method of new species hybrid cymbidium - Google Patents
Culture method of new species hybrid cymbidium Download PDFInfo
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- CN101455178A CN101455178A CNA2007101508853A CN200710150885A CN101455178A CN 101455178 A CN101455178 A CN 101455178A CN A2007101508853 A CNA2007101508853 A CN A2007101508853A CN 200710150885 A CN200710150885 A CN 200710150885A CN 101455178 A CN101455178 A CN 101455178A
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Abstract
The present invention relating to the field of flower technology provides a method of cultivating a cymbidium hybridum of a new variety. The cultivating method comprises steps of aseptic inoculation, aseptic culture in laboratory, transplanting, small seedling cultivating, big seedling cultivating and flowering cultivating. The method of cultivating a cymbidium hybridum of a new variety has a simple operation and a low cost, can cultivate the cymbidium hybridum variety that is drought-resistant, heat-resisting and suitable for native planting and breeding. Therefore, in order to satisfy the market demand,the cymbidium hybridum can be popularized and spread in China market.
Description
Technical field
The present invention relates to field of flower technology, especially a kind of cultural method of new varieties hybrid cymbidium.
Background technology
Hybrid cymbidium has another name called Cymbidium hookerianum, yellow cicada orchid, is a kind of good living tropically orchid.It with the numerous crowd of designs and varieties, spend big and many, the florescence of blossoming is celebrated for a long time, bloom can from November until May in next year.Current, the hybrid cymbidium in the Spring Festival flowers is many from Korea S's import, and price is higher.In fact, the cultivation difficulty of hybrid cymbidium as long as understand its habit, gives relative measures unlike other orchid height, can successfully cultivate.Hybrid cymbidium hybrid generation phenotype is analyzed and cultivated, through further screening, the big flowers orchid species that acquisition has drought-resistant heat-resistant quality, is suitable for the native country plantation and cultivates, the demand of catering to market makes hybrid cymbidium be popularized on Chinese market and promote.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of cultural method of new varieties hybrid cymbidium is provided, this method is simple to operate, and is with low cost, can make hybrid cymbidium be popularized on Chinese market and promote.
The present invention is achieved through the following technical solutions:
1. the cultural method of a new varieties hybrid cymbidium, this cultural method may further comprise the steps:
(1) aseptic inoculation: get a pollination 4-10 month uncracked capsule and in flowing water, rinse well, wipe out up and down about each 0.5-2cm of two ends, in superclean bench,, use 0.1% HgCl with 75% alcohol-pickled 1-2min
2Sterilization 10-15min with aseptic water washing 4-7 time, gets the aseptic operation cutter capsule is vertically cut away half, and is sprinkling upon on the medium with the aseptic sub-general seed wherein of taking the photograph, and utilizes the moisture of media surface that seed is rocked it is evenly distributed as far as possible;
Above-mentioned medium is the 1/2MS medium of additional NAA 0.5mg/L;
(2) aseptic culture in laboratory: under 25 ℃ of conditions, secretly cultivate, hybrid seed is sprouted successively behind the 50-70d, form the spherical protocorm of white circular of the about 0.5mm of diameter, the close hair of taking root of appearance, after this under the 8000-12000lx illumination condition, cultivate, protocorm transfers to green and constantly grows up after a couple of days, protocorm is taken out from medium, cut into fritter, be seeded in the MS medium that adds 6-benzyl aminoadenine 2mg/L and methyl 0.2mg/L, make protocorm propagation, protocorm is continued to cultivate in proliferated culture medium, form bud on the protocorm top behind the 18-25d, partly change root bastem;
(3) transplant: will have the medium bottle cap of the seedling of taking root to open, hardening 3-5d in the cultivation greenhouse, be with head thin sebific duct long forceps seedling is pressed from both sides out from bottle, clean the medium of shoot root portion, seedling need be with 0.05% liquor potassic permanganate immersion 4-7min;
With the thick clean cobble of seedling-cultivating tray bottom shop one deck 3-5cm, repave the liver moss of one deck 1-3cm, each seedling root neatly is emitted on Miao Panzhong with behind the liver moss parcel, require liver moss to be wrapping to the rhizome junction, each seedling dish 50-60 test-tube plantlet of planting planted to finish to water and is placed on the cultivating stand in greenhouse after permeable;
(4) seedling is cultivated: continue under the following conditions to cultivate:
In the daytime: optimum temperature 18-22 ℃, can not surpass 22 ℃, can not be lower than 15 ℃;
Night: optimum temperature 12-18 ℃, can not surpass 18 ℃, can not be lower than 10 ℃.
Intensity of illumination: 8000-12000lx.
Humidity remains on 60-70%.
(5) seedlings are cultivated: height of seedling 15-20cm behind the test-tube seedling transplanting 50-70d, at this moment need the seedling branch is planted in the nutritive cube, adopt nutritive cube, bottom pad one deck cobble, put into one deck Chinese larch bark, put into 10 Magamp K slow-release fertilizers then, matrix adopts particulate Chinese larch bark, before transplanting bark is soaked 20-25h, seedling is taken out from the seedling dish, with the whole embeddings of root, water permeable with soaked potanin larch bark, shading 90%, air humidity remains on 80-90% in 1 week, temperature between daytime 22-30 ℃, 18-22 ℃ of night, 1 week back assurance intensity of illumination 20000-30000lx, air humidity remains on 70%-80%.
(6) strain of blooming is cultivated:
Optimum humidity is 60%-70%, the highlyest surpasses 80%, minimumly is not lower than 50%.
Intensity of illumination: 25000-35000lx.
In the daytime: optimum temperature 22-25 ℃, can not surpass 30 ℃, can not be lower than 18 ℃;
Night: optimum temperature 15-20 ℃, can not surpass 25 ℃, can not be lower than 10 ℃.
Potted plant hybrid cymbidium matrix uses concentration below 0.1% with the mixture of fern root, liver moss and bark piece, and nitrogen, phosphorus, potassium ratio are 1: 1: 1 composite fertilizer, spray weekly 1 time.
And described intensity of illumination should be kept 8-13h every day.
Advantage of the present invention and beneficial effect are:
The cultural method of this new varieties hybrid cymbidium is simple to operate, and is with low cost, can turn out drought-resistant heat-resisting, and the big flowers orchid species that is suitable for native country plantation and cultivates, the demand of catering to market makes hybrid cymbidium be popularized on Chinese market and promote.
Embodiment
The present invention is described in further detail by following examples, but the technology contents that present embodiment is narrated is illustrative, rather than determinate, should not limit to protection scope of the present invention according to this.
A kind of cultural method of new varieties hybrid cymbidium, this cultural method may further comprise the steps:
(1) aseptic inoculation: get 6 months uncracked capsules of pollination and in flowing water, rinse well, wipe out up and down each 1cm of two ends, in superclean bench,, use 0.1% HgCl with 75% alcohol-pickled 1min
2Sterilization 11min with aseptic water washing 5 times, gets the aseptic operation cutter capsule is vertically cut away half, and is sprinkling upon on the medium with the aseptic son general seed wherein of taking the photograph, and utilizes a little moisture of media surface that seed is rocked it is evenly distributed as far as possible.
Above-mentioned medium is the 1/2MS medium of additional NAA 0.5mg/L.
(2) aseptic culture in laboratory: under 25 ℃ of conditions, secretly cultivate, hybrid seed is sprouted successively behind the 60d, form the spherical protocorm of white circular of the about 0.5mm of diameter, the close hair of taking root of appearance, after this under the 10000lx illumination condition, cultivate, protocorm transfers to green and constantly grows up after a couple of days, protocorm is taken out from medium, cut into fritter, be seeded in the MS medium that adds 6-benzyl aminoadenine 2mg/L and methyl 0.2mg/L, make protocorm propagation, protocorm is continued to cultivate in proliferated culture medium, form bud on the protocorm top through 20d, partly change root bastem.
In the daytime: optimum temperature 22-28 ℃, 18-30 ℃ of acceptable temperature range;
Night: optimum temperature 18-22 ℃, 15-25 ℃ of acceptable temperature range.
In the daytime: optimum temperature 18-22 ℃, can not surpass 22 ℃, can not be lower than 15 ℃;
Night: optimum temperature 12-18 ℃, can not surpass 18 ℃, can not be lower than 10 ℃.
(3) transplant: will have the medium bottle cap of the seedling of taking root to open, hardening 4d in the cultivation greenhouse, be with head thin sebific duct long forceps seedling is pressed from both sides out from bottle, clean the medium of shoot root portion, seedling need soak 5min with potassium permanganate weak solution (0.05%), can improve transplanting survival rate.
With 40cm * 30cm, the thick clean cobble of plastic seeding culturing tray bottom shop one deck 4cm of high 12cm, repave the liver moss of one deck 2cm, each seedling root neatly is emitted on Miao Panzhong with behind the liver moss parcel, require liver moss to be wrapping to the rhizome junction, each seedling dish 45 test-tube plantlets of planting are planted to finish to water and are placed on the cultivating stand in greenhouse after permeable.
(4) seedling is cultivated: continue under the following conditions to cultivate:
In the daytime: 22 ℃;
Night: 15 ℃;
Illumination: 10000lx;
Humidity remains on 65%;
(5) seedlings are cultivated: height of seedling 15-20cm behind the test-tube seedling transplanting 60d, at this moment need the seedling branch is planted in the nutritive cube, adopt 8cm black nutritive alms bowl, bottom pad one deck cobble is put into skim Chinese larch bark, puts into 10 Magamp K slow-release fertilizers then, matrix adopts particulate Chinese larch bark, before transplanting bark is soaked 24h. seedling is taken out from the seedling dish, with the whole embeddings of root, water permeable with soaked potanin larch bark, shading 90%, air humidity remains on 90% in 1 week, temperature between daytime 25 ℃, 20 ℃ of nights, 1 week back assurance luminous intensity 30000lx, air humidity remains on 70%,, do not make matrix drying in the basin.
(6) strain of blooming is cultivated:
Optimum humidity is 60%-70%, the highlyest surpasses 80%, minimumly is not lower than 50%.
Optimal light impinges upon 25000-35000lx.
In the daytime: optimum temperature 22-25 ℃, can not surpass 28 ℃, can not be lower than 18 ℃;
Night: optimum temperature 15-20 ℃, can not surpass 22 ℃, can not be lower than 10 ℃.
The high tube of potted plant hybrid cymbidium 15cm-20cm commonly used flowerpot, every potted plant 2-4 young plant, the mixture of potting media fern root, liver moss and bark piece, vegetative period, every first quarter moon fertilising was 1 time, also usable concentration is below 0.1%, and nitrogen, phosphorus, potassium ratio are 1: 1: 1 composite fertilizer, sprays weekly 1 time.It is loose that pseudobulb is enriched, and just can impel flower bud differentiation, blooms more.But the kind that rudiment power is strong will be controlled the leaf bud growth, and nutrient is too much consumed, and just must carry out bud picking, a pseudobulb given birth to a leaf bud.
Should keep every day more than the above-mentioned intensity of illumination 10h, minimum will keeping more than the 8h, otherwise growth result will significantly reduce.
Claims (2)
1. the cultural method of a new varieties hybrid cymbidium, it is characterized in that: this cultural method may further comprise the steps:
(1) aseptic inoculation: get a pollination 4-10 month uncracked capsule and in flowing water, rinse well, wipe out up and down about each 0.5-2cm of two ends, in superclean bench,, use 0.1%HgCl with 75% alcohol-pickled 1-2min
2Sterilization 10-15min with aseptic water washing 4-7 time, gets the aseptic operation cutter capsule is vertically cut away half, and is sprinkling upon on the medium with the aseptic sub-general seed wherein of taking the photograph, and utilizes the moisture of media surface that seed is rocked it is evenly distributed as far as possible;
Above-mentioned medium is the 1/2MS medium of additional NAA 0.5mg/L;
(2) aseptic culture in laboratory: under 25 ℃ of conditions, secretly cultivate, hybrid seed is sprouted successively behind the 50-70d, form the spherical protocorm of white circular of the about 0.5mm of diameter, the close hair of taking root of appearance, after this under the 8000-120001x illumination condition, cultivate, protocorm transfers to green and constantly grows up after a couple of days, protocorm is taken out from medium, cut into fritter, be seeded in the MS medium that adds 6-benzyl aminoadenine 2mg/L and methyl 0.2mg/L, make protocorm propagation, protocorm is continued to cultivate in proliferated culture medium, form bud on the protocorm top behind the 18-25d, partly change root bastem;
(3) transplant: will have the medium bottle cap of the seedling of taking root to open, hardening 3-5d in the cultivation greenhouse, be with head thin sebific duct long forceps seedling is pressed from both sides out from bottle, clean the medium of shoot root portion, seedling need be with 0.05% liquor potassic permanganate immersion 4-7min;
With the thick clean cobble of seedling-cultivating tray bottom shop one deck 3-5cm, repave the liver moss of one deck 1-3cm, each seedling root neatly is emitted on Miao Panzhong with behind the liver moss parcel, require liver moss to be wrapping to the rhizome junction, each seedling dish 50-60 test-tube plantlet of planting planted to finish to water and is placed on the cultivating stand in greenhouse after permeable;
(4) seedling is cultivated: continue under the following conditions to cultivate;
In the daytime: optimum temperature 18-22 ℃, can not surpass 22 ℃, can not be lower than 15 ℃;
Night: optimum temperature 12-18 ℃, can not surpass 18 ℃, can not be lower than 10 ℃.
Intensity of illumination: 8 000-12000 1x.
Humidity remains on 60-70%.
(5) seedlings are cultivated: height of seedling 15-20cm behind the test-tube seedling transplanting 50-70d, at this moment need the seedling branch is planted in the nutritive cube, adopt nutritive cube, bottom pad one deck cobble, put into one deck Chinese larch bark, put into 10 Magamp K slow-release fertilizers then, matrix adopts particulate Chinese larch bark, before transplanting bark is soaked 20-25h, seedling is taken out from the seedling dish, with the whole embeddings of root, water permeable with soaked potanin larch bark, shading 90%, air humidity remains on 80-90% in 1 week, temperature between daytime 22-30 ℃, 18-22 ℃ of night, 1 week back assurance intensity of illumination 20000-300001x, air humidity remains on 70%-80%.
(6) strain of blooming is cultivated:
Optimum humidity is 60%-70%, the highlyest surpasses 80%, minimumly is not lower than 50%.
Intensity of illumination: 25 000-35000 1x.
In the daytime: optimum temperature 22-25 ℃, can not surpass 30 ℃, can not be lower than 18 ℃;
Night: optimum temperature 15-20 ℃, can not surpass 25 ℃, can not be lower than 10 ℃.
Potted plant hybrid cymbidium matrix uses concentration below 0.1% with the mixture of fern root, liver moss and bark piece, and nitrogen, phosphorus, potassium ratio are 1: 1: 1 composite fertilizer, spray weekly 1 time.
2. the cultural method of new varieties hybrid cymbidium according to claim 1 is characterized in that: described intensity of illumination all should be kept 8-13h every day.
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| CNA2007101508853A CN101455178A (en) | 2007-12-11 | 2007-12-11 | Culture method of new species hybrid cymbidium |
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| CN102283127A (en) * | 2011-07-21 | 2011-12-21 | 福建省亚热带植物研究所 | Culture medium synchronously used for induction and multiplication of Cymbidium hybridum stem tip Caespitose shoots and use method thereof |
| CN102487800A (en) * | 2011-12-23 | 2012-06-13 | 贵州省农作物品种资源研究所 | Artificial cultivation method for wild paphiopedilum hirsutissimum |
| CN102511293A (en) * | 2011-12-29 | 2012-06-27 | 贵州省农作物品种资源研究所 | Artificial cultivation method for wild Coelogyne fimbriata |
| CN102668955A (en) * | 2012-05-25 | 2012-09-19 | 北京林大林业科技股份有限公司 | Production method of novel flower environment-friendly medium |
| CN102668954A (en) * | 2012-05-25 | 2012-09-19 | 北京林大林业科技股份有限公司 | Method for changing flower shape |
| CN102754599A (en) * | 2012-07-16 | 2012-10-31 | 上海市农业科学院 | Method for quickly breeding cymbidium hybridium by use of root inducing protocorm |
| CN103270878A (en) * | 2013-05-28 | 2013-09-04 | 磐安县大盘山兰蕙专业合作社 | Wild orchid transfer and culture method |
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| CN103430842A (en) * | 2013-08-07 | 2013-12-11 | 广东省农业科学院环境园艺研究所 | Quick propagation method of hybrid orchid tissue culture |
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| CN104521713A (en) * | 2014-12-31 | 2015-04-22 | 吴中区金庭小美华家庭农场 | Cultivation method for cymbidium |
| CN105409516A (en) * | 2015-11-16 | 2016-03-23 | 浙江农林大学 | Year-round production and cultivation method of Cymbidium hybridum |
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| CN102283127A (en) * | 2011-07-21 | 2011-12-21 | 福建省亚热带植物研究所 | Culture medium synchronously used for induction and multiplication of Cymbidium hybridum stem tip Caespitose shoots and use method thereof |
| CN102487800A (en) * | 2011-12-23 | 2012-06-13 | 贵州省农作物品种资源研究所 | Artificial cultivation method for wild paphiopedilum hirsutissimum |
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| CN102668955A (en) * | 2012-05-25 | 2012-09-19 | 北京林大林业科技股份有限公司 | Production method of novel flower environment-friendly medium |
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| CN102668955B (en) * | 2012-05-25 | 2013-05-08 | 北京林大林业科技股份有限公司 | Production method of novel flower environment-friendly medium |
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| CN102754599B (en) * | 2012-07-16 | 2013-09-18 | 上海市农业科学院 | Method for quickly breeding cymbidium hybridium by use of root inducing protocorm |
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| CN103270878B (en) * | 2013-05-28 | 2015-08-05 | 磐安县大盘山兰蕙专业合作社 | One is gone down the hill blue transformation cultural method |
| CN103329807B (en) * | 2013-07-16 | 2015-04-15 | 上海市农业科学院 | Method for rapidly breeding hybrid orchid by root, as well as culture medium |
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| CN105409516A (en) * | 2015-11-16 | 2016-03-23 | 浙江农林大学 | Year-round production and cultivation method of Cymbidium hybridum |
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| CN105766656A (en) * | 2016-04-28 | 2016-07-20 | 广西壮族自治区药用植物园 | One-step seedling method for rapid propagation of pseudobulbe of phaius tankervilleae |
| CN105766656B (en) * | 2016-04-28 | 2018-02-27 | 广西壮族自治区药用植物园 | The forming seedling through one step culture method that mun orchid pseudobulb is quickly bred |
| CN106069159A (en) * | 2016-07-22 | 2016-11-09 | 深圳市仙湖植物园管理处 | The inoculation method of blood aspidistra |
| CN106069704A (en) * | 2016-07-22 | 2016-11-09 | 董忠 | Full-automatic photovoltaic vegetable planting machine |
| CN106212231A (en) * | 2016-07-27 | 2016-12-14 | 江苏明盛农林生态科技有限公司 | A kind of method cultivating white edge Herba Cymbidii Goeringii |
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| CN106258960B (en) * | 2016-08-10 | 2018-08-31 | 山东省农作物种质资源中心 | A kind of orchid seed sprouting quick-breeding method |
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