CN101379185B - 新方法 - Google Patents

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Publication number
CN101379185B
CN101379185B CN200780004615XA CN200780004615A CN101379185B CN 101379185 B CN101379185 B CN 101379185B CN 200780004615X A CN200780004615X A CN 200780004615XA CN 200780004615 A CN200780004615 A CN 200780004615A CN 101379185 B CN101379185 B CN 101379185B
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gene
thymidine
thf
plasmid
coli
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Chinese (zh)
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CN101379185A (zh
Inventor
戴维·M·安德森
安德鲁·J·科利斯
刘林
瑟吉·波德科维罗夫
克里斯托弗·普雷斯顿
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Glaxo Group Ltd
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Glaxo Group Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/385—Pyrimidine nucleosides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Virology (AREA)
  • Public Health (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • AIDS & HIV (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200780004615XA 2006-02-06 2007-02-05 新方法 Active CN101379185B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0602339.4 2006-02-06
GBGB0602339.4A GB0602339D0 (en) 2006-02-06 2006-02-06 Novel process
PCT/EP2007/051072 WO2007090810A1 (en) 2006-02-06 2007-02-05 Novel process

Publications (2)

Publication Number Publication Date
CN101379185A CN101379185A (zh) 2009-03-04
CN101379185B true CN101379185B (zh) 2012-08-29

Family

ID=36101118

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200780004615XA Active CN101379185B (zh) 2006-02-06 2007-02-05 新方法

Country Status (9)

Country Link
US (1) US8263384B2 (https=)
EP (2) EP1981972B1 (https=)
JP (1) JP5230447B2 (https=)
KR (1) KR101371954B1 (https=)
CN (1) CN101379185B (https=)
ES (1) ES2410857T3 (https=)
GB (1) GB0602339D0 (https=)
SI (1) SI1981972T1 (https=)
WO (1) WO2007090810A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7572065B2 (en) 2007-01-24 2009-08-11 Adc Telecommunications, Inc. Hardened fiber optic connector
JP2010518837A (ja) * 2007-02-19 2010-06-03 エボニック デグサ ゲーエムベーハー ホルメート−ｔｈｆ−シンテターゼおよび／またはグリシン開裂活性を有するコリネ型細菌
CN113755416B (zh) * 2020-06-05 2023-08-15 苏州华赛生物工程技术有限公司 具有新合成路径生产β-胸苷的重组微生物及生产β-胸苷的方法
CN111748537B (zh) * 2020-08-04 2021-11-26 浙江华睿生物技术有限公司 一种尿苷磷酸酶突变体及其应用
KR102398032B1 (ko) * 2020-09-22 2022-05-19 비피진 주식회사 암 치료를 위한 5''-뉴클레오티다아제 변이체
CN112126666B (zh) * 2020-09-22 2022-04-08 廊坊梅花生物技术开发有限公司 核苷高产菌及其构建方法与应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE68916708T2 (de) 1988-05-31 1994-12-01 Zeneca Ltd Herstellung eines Desoxyribonucleosids.
US5213972A (en) * 1989-12-08 1993-05-25 Chemgen Corporation Fermentation process for the production of pyrimidine deoxyribonucleosides
JP3916345B2 (ja) 1999-06-26 2007-05-16 均 西谷 両開きドア
DE60035781T2 (de) * 1999-07-01 2008-08-07 Glaxo Group Ltd., Greenford Vektoren, zellen und verfahren zur herstellung von pyrimidindeoxyribonucleosiden

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Sarla Purohit and Christopher K.Mathews.Nucleotide sequence reveals overlap between T4 phage genes encoding dihydrofolate reductase and thymidylate synthase.《The Journal of Biological Chemistry》.1984,第259卷(第10期),6261-6266. *
沈荣坤等.应用大肠杆菌的核苷磷酸化酶合成胸苷.《华东理工大学学报》.1996,第22卷(第6期),701-706. *

Also Published As

Publication number Publication date
GB0602339D0 (en) 2006-03-15
EP2287294A1 (en) 2011-02-23
JP5230447B2 (ja) 2013-07-10
EP1981972A1 (en) 2008-10-22
WO2007090810A1 (en) 2007-08-16
US20090081735A1 (en) 2009-03-26
JP2009525738A (ja) 2009-07-16
KR101371954B1 (ko) 2014-03-07
US8263384B2 (en) 2012-09-11
KR20080093118A (ko) 2008-10-20
EP1981972B1 (en) 2013-03-20
ES2410857T3 (es) 2013-07-03
CN101379185A (zh) 2009-03-04
SI1981972T1 (sl) 2013-07-31

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