CN101313664A - Process for preparing corn hybrid Liangyu 88 - Google Patents
Process for preparing corn hybrid Liangyu 88 Download PDFInfo
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- CN101313664A CN101313664A CNA2008100122183A CN200810012218A CN101313664A CN 101313664 A CN101313664 A CN 101313664A CN A2008100122183 A CNA2008100122183 A CN A2008100122183A CN 200810012218 A CN200810012218 A CN 200810012218A CN 101313664 A CN101313664 A CN 101313664A
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Abstract
A method for making corn hybrid Liangyu No. 88 comprises the following steps that: a male parent and a female parent are selected; the female parent is selected from a self-bred inbred line M54, while the male parent is selected from a self-bred inbred line S12; a first stage of self-bred inbred line S12 male parent is sown with the sowing quantity occupying 50 percent of the total sowing quantity of the male parent; when the first stage male parent takes root, a second stage of self-bred inbred line S12 male parent is sown with the sowing quantity occupying 50 percent of the total sowing quantity of the male parent; when the second stage male parent takes root, the female parent is sown with the sowing ratio of the male parent to the female parent equal to 1:5; a safety partition zone is arranged between the male parent and the female parent with the distance of the partition zone more than or equal to 300m, and no non-male parent corn is allowed to be sown in the partition zone; 75,000 to 90,000 seedlings are reserved in every hectare; all abnormal seedlings, heterologous seedlings, weak seedlings and sick seedlings are pulled out not according with parental typical characteristics before seedling stage, jointing stage and tasseling stage; and before the female parent spins, a male ear of the female parent is taken out in time along with a leaf so as to prevent the occurrence of selfing phenomenon, thereby breeding a new corn variety, i.e. Liangyu No. 88. The output of the bred Liangyu No. 88 is more than 20 percent higher than the prior production capacity, and the bred Liangyu No. 88 has the advantages of stable output, high quality and wide adaptability, etc.
Description
Technical field:
The invention belongs to corn hybrid use of advantage field.
Background technology:
Corn is China's staple food crop, its year cultivated area and gross yield all name occupy second.Be very important grain ration, feed, the raw material of industry, fuel, medical material, in national economy is produced, occupy critical role, brought into play huge effect for ensureing national food safety.And the utilization of corn hybrid advantage effectively raises the per unit area yield of corn, is the improvement corn quality, increases a kind of very important technological means and the highly efficient and productive approach of corn yield.
Since the eighties of last century the eighties, China's Maize Production had both turned to use single cross hybrid, for this reason, it is breeding goal that corn breeding research also turns to the good selfing of comprehensive agronomy proterties good with the seed selection proterties, that horizontal resistance is strong, coordinate force is high synchronously, is intended to assembly and goes out high yield, stable yields, high-quality, many anti-superior corn hybrid new breeds.
Summary of the invention:
The object of the present invention is to provide a kind of new method that is higher than the production corn hybrid seed of existing production capacity more than 20%.
The technical scheme that adopts is:
It is maternal adopting known self-fertile selfing line M 54, self-fertile inbred line S12 is that male parent is hybridized, combine " good beautiful No. 88 " corn hybridization new varieties, take to be provided with secure isolation zone, carry out proper seeding, rational close planting, suitable row ratio, roguing, the maternal castration, hybrid seed production is carried out in multinomial measures such as in good time results.
Safety described in the aforementioned production method is isolated into distance isolation, and isolation distance must guarantee must not plant non-male parent corn in the isolated area more than 300 meters;
Proper seeding described in the aforementioned production method accounts for 50% for broadcasting the first phase male parent earlier, broadcasts the second stage of male parent (50%) after the first phase male parent is taken root, and after the second stage of male parent is taken root, broadcasts female parent again;
Rational close planting described in the aforementioned production method is per hectare 75000～90000 strains of keeping a full stand of seedings, and can obtain per hectare and reach hybrid seed yield more than 7500 kilograms;
Suitable row described in the aforementioned production method is more capable of being 1: 5 than what be meant this sowing of father and mother;
Roguing described in the aforementioned production method is meant in seedling stage, shooting stage, take out and will not meet the assorted strain of parent's characteristic feature characteristic, different strain, weak strain, diseased plant male early stage and all pull out, and guarantees the seed production purity and the quality of production.
Female parent described in the aforementioned production method castrates and is meant before female parent is weaved silk in time, totally, thoroughly maternal tassel band leaf is extracted, prevent the selfing phenomenon, influence the purity of hybrid seed.
The good beautiful No. 88 maternal selfing line M 54 of corn hybrid seed is by after introducing american corn crossbreed 1160R, 1140D, 1132X and the mixed powder of German corn hybrid seed KW456 enforcement equivalent, hybridize with its fine individual plant respectively again, carried out monoseeding in 1 year, field strict rejecting selfing strain, select the strong fine individual plant of hybrid vigour to carry out adopting pedigree method after the selfing, continuous 8 generations selfing is bred.
The male parent inbred line S12 that corn hybrid seed is good beautiful No. 88 is that continuous selfing is bred through 6 generations with after tucking in H201 * pellet 340/ and tucking in H204 and hybridize.
Good beautiful No. 88 feature of corn hybrid seed described in the present invention is:
1, tree characteristics: well developed root system, seedling leaf sheath purple, early onset is good, and it is strong to encircle native ability, is suitable for smart amount of mechanization or monoseeding.Become plant height 270-292cm, fringe position 90-99cm, complete stool blade 20-21 sheet.The fringe lower blade is open and flat, and fringe upper blade joint is long, leaf is dredged, upper punch, the delicate and pretty compactness of plant type, and the tassel branch is few, and lodging resistance is good, anti-disease, anti-lean, drought resistance is strong.
2, fruit ear and grain characters: long cartridge type in the fruit ear, spike length 19-21cm, the thick 5.0-5.4cm of fringe, tassel row number 16～20 row.The seed yellow, dark horse flute profile, the cob redness, about thousand kernel weight 320-350g, seed-producing rate 88-90% is about unit weight 750g.
3, resistance: the high Chinese People's Anti-Japanese Military and Political College, helminthosporium maydis, graywall, Curvularia lunata leaf spot, head smut, stem rot, bacterial wilt, virus disease.
4, the phenophase: breeding time, east North China spring sowing was 128-130 days, the Yellow River and Huai He River sea summer sowing 96-103 days.Kind of a Zheng Dan 958 compares short 2-3 days with contrast.
5, output performance
Provincial regional test
Participated in late-maturing group of Liaoning Province's preliminary experiment in 2006, in 426 kinds of participating in the experiment then, comparison is shone under kind of 4 days the situation of red beautiful No. 39 precocities, per mu yield 744.8kg, and kind of a volume increase 16.7% is shone in comparison, and output occupies the 2nd.
Participated in the late-maturing B group of Liaoning Province's regional test in 2007, per mu yield 683.1kg, kind of red beautiful No. 39 per mu yield 585.7kg are shone in comparison, volume increase 16.6%, output occupies the 2nd.
Participated in the main producing region preliminary experiment of national corn in 2007, with the recommended promotion of the primary outstanding representation of its overall ranking to the regional test in Si Da district, national corn main producing region:
(1) with 785.7 kilograms of per mu yields, than CK volume increase 4.7%, the achievement of the position of being number three is promoted to eastern North China regional test.
(2) with 628.5 kilograms of per mu yields, than CK volume increase 3.64%, the achievement of the position of being number four is promoted to the test of the Yellow River and Huai He River sea region.
(3) with 538.9 kilograms of per mu yields, than CK volume increase 5.11%, the achievement of the position of being number three is promoted to the test of southwestern area.
(4) with 986.3 kilograms of per mu yields, than CK volume increase 18.48%, the achievement of the position of ranking the first is promoted to the northwest regional test.
6, resistance is identified:
Prove through the qualification result of participating in the experiment in 2007: good beautiful No. 88 high resistant to lodging, high anti----in the anti-main disease of multiple corn:
Table one resists down excellent and the disease resistance performance for good beautiful No. 88 corns
| The zone | Breeding time (my god) | Lodging % | Roll over % | Leaf blight (level) | Helminthosporium maydis (level) | Graywall (level) | Curved spore graywall (level) | Banded sclerotial blight (level) | Stem rot % | Head smut % | Smut % | Anti-maize borer (level) |
| Liaoning Province | 130 | 0.2 | 0.0 | 1-3 | 1 | 1-3 | 1-3 | 4.8 | 0.1 | 1 | ||
| North China, east | 129.8 | 0.8 | 0.6 | 1.4 | 0.6 | 1.6 | ||||||
| The Yellow River and Huai He River sea | 100.9 | 2.0 | 1.7 | 1.7 | 1.3 | 7.7 | ||||||
| The southwest district | 110.8 | 6.0 | 0.3 | 1.0 | 2.0 | |||||||
| The northwest | 132.8 | 0.0 | 0.0 | 1.0 | 0.0 | |||||||
| Henan Province | 99 | 6.2 | 1.5 | 2.8 | 6.8 | 0.5 |
7, culture technique main points
(1), choosing ground and fertilising
The anti-close type kind of suitable smart amount of this corn hybridization kind or monoseeding, yield stability is good, and yielding ability is strong, should select the plantation of medium above fertility plot.Mu is executed farmyard manure 2000～3000kg, and composite fertilizer 25kg makees base fertilizer, and two ammonium 5kg do seed manure, and the shooting stage imposes urea 25～30kg, or the disposable three material composite fertilizer 50kg that apply.
(2), plantation form and density
This crossbreed is planted clearly, all can interplant, and the clear mu zone, eastern North China of keeping a full stand of seedings of planting is the 3800-4000 strain, and the Yellow River and Huai He River sea region is the 4000-4500 strain.
(3), the extermination of disease and insect pest
Can adopt seed pelleting dressing seed control soil insect during sowing, the typhon mouth phase is with granule or oophagous trichogrammae control of maize snout moth's larva.
(4), seeding technique
The production of hybrid seeds will be selected the better-than-average fertility plot of irrigation conditions, isolation distance guarantees more than 300 meters, father and mother's one's own profession was advisable than 1: 5, should broadcast the first phase male parent earlier, treat after 3-5 days to broadcast the second stage of male parent after the first phase male parent is taken root, treat to broadcast female parent again after the second stage of male parent is taken root, the per hectare 75000-90000 strain of keeping a full stand of seedings all can, general plot yield level can guarantee 500 kilograms, and the high yield plot can reach more than 600 kilograms.
(5), suitable planting area
According to the result of the test checking in national corn main producing region prerun, the examination of provinces and regions, Liaoning, Beijing, Hebei province, Henan Province, Shandong Province, Shaanxi Province, this kind can be in North China, China east, the Yellow River and Huai He River sea, southwest, northwest, Beijing-Tianjin-Tangshan etc. regional suitable planting Zheng Dan 958 and earlier beautiful 335 area test widely, demonstrate and plant experimentally.
No. 88 corn yield height of the breeding of cultivating are higher than existing production capacity more than 20%, and have stable yields, high-quality, advantages such as wide adaptability.
Description of drawings
Fig. 1 is good beautiful No. 88 corn hybridizations promotion collection of illustrative plates of participating in the experiment.
Detailed description of main elements:
1 is (U.S.) 1160R, 1140D, 1132X, (moral) KW456;
2 tuck in H204 for tucking in H201 * pellet 340/;
3 is the test of 2004 Nian Dong companies anharmonic ratio wide in variety;
4 is DL05 (good beautiful No. 88) in 2005;
5 is multiple spot test inside and outside the province in 2006;
6 is Liaoning Province's preliminary experiment in 2006;
7 is Liaoning Province's regional test in 2007; Country's corn main producing region preliminary experiment; Highly dense group of preliminary experiment of Beijing's summer sowing; Highly dense group of preliminary experiment of Henan Province's summer sowing; Highly dense group of preliminary experiment of Shandong Province's summer sowing;
8 is winter in 2008 Liaoning Province's regional test; Liaoning Province's production test; The four big area tests of country corn main producing region; The test of zone, Beijing; Henan Province's regional test; Shandong Province's regional test;
9 is 8 generations of M54 selfing;
10 is 6 generations of S121 selfing.
Embodiment
Embodiment one
Produced good beautiful No. 88 corn hybrid seeds in Jiuquan, Gansu Province area in 2007, production of hybrid seeds district adopts distance isolation, and isolation distance is 300 meters, i.e. the maize planting not in 300 meters in production of hybrid seeds district;
Sowing on April 28th, 2007 first phase male parent is whenever broadcast 3 meters, and empty 3 meters, the first phase male parent shows money or valuables one carries unintentionally, takes root during May 4, broadcasts the second stage of male parent at former empty 3 meters, when Mays 9, the Nikkei field investigation second phase, male parent was taken root, begins to broadcast female parent, male parent sowing 1 row, maternal sowing 5 row.Planting density is per hectare 75000 strains, and seeding row spacing is 24cm * 55cm, and it is 62500 strains that female parent is kept a full stand of seedings, and it is 12500 strains that male parent is kept a full stand of seedings.
May 29 was carried out thinning, and every cave keeps a strain, and unnecessary plant is removed, and kept the healthy seedling of neat and consistent.June 20---before July 15, female parent was taken out hero, carry out rogueing at twice and rogue, pull out the assorted strain that does not meet the parental inbred line characteristic feature, different strain, weak strain, diseased plant.July 20---carry out artificial emasculation every day July 28, promptly before female parent is weaved silk, in time maternal tassel all pulled out.After treating that pollination finishes, in time male parent is cut off, September 26---the fruit ear of female parent gathered in the crops in 28 in good time, carry out nature and dry or implements after the artificial drying threshing and put in storage and carry out safe storage.
Good beautiful No. 88 per mu yields were 500 kilograms, reached 32% than its average amount of increase in production through comparing for 378 kilograms/mu with first beautiful 335 hybrid seed yields of similar type kind in 2007.
Claims (4)
1, good beautiful No. 88 production method of a kind of corn hybrid seed is characterized in that:
1) select male parent and female parent, female parent is selected the self-fertile selfing line M 54 for use, and male parent is selected self-fertile inbred line S12 for use;
2) sow first phase self-fertile inbred line S12 male parent earlier, seeding quantity accounts for male parent sowing total amount 50%, the second stage of self-fertile inbred line S12 male parent of sowing after the first phase male parent is taken root, seeding quantity is 50% of a male parent sowing total amount, after the second stage of male parent is taken root, sowing is maternal again, and male parent and maternal seeding quantity proportioning are 1: 5;
3) between male parent and the female parent secure isolation zone is set, isolated area distance 〉=300m must not plant non-male parent corn in the isolated area;
4) the per hectare 75000-90000 strain of keeping a full stand of seedings;
5) will not meeting the assorted strain of parent's characteristic feature, different strain, weak strain, diseased plant before seedling stage, shooting stage, tasseling stage all extracts;
6) in time maternal tassel band leaf is extracted before female parent is weaved silk, prevent the selfing phenomenon, the corn variety of cultivating is good beautiful No. 88.
2, good beautiful No. 88 production method of a kind of corn hybrid seed according to claim 1, it is characterized in that: execute farmyard manure 2000-3000kg for every mu, composite fertilizer 25kg makees base fertilizer, and diamines 5kg makes seed manure, shooting stage imposes urea 25-30kg, or the disposable three material composite fertilizer 50kg that apply.
3, good beautiful No. 88 production method of a kind of corn hybrid seed according to claim 1 is characterized in that: sow the second stage of male parent after 3-5 days in sowing first phase male parent.
4, good beautiful No. 88 production method of a kind of corn hybrid seed according to claim 1 is characterized in that:
Every sowing 3m during sowing first phase male parent, empty 3m, be separated by 5 days in the second stage of male parent of former empty 3m place sowing, it is maternal to begin sowing after the field investigation second phase, male parent was taken root, male parent sowing 1 row, maternal sowing 5 row, strain density is per hectare 75000 strains, and seeding row spacing is 24cm * 55cm, and it is 62500 strains that female parent is kept a full stand of seedings, it is 12500 strains that male parent is kept a full stand of seedings, the maternal kind after 20 days carried out the field seedling, and unnecessary plant is removed in the strain of keeping a full stand of seedings of every cave, the healthy seedling that keeps neat and consistent, before female parent is taken out hero, carry out rogueing at twice and rogue, pull out the assorted strain that does not meet the parental inbred line characteristic feature, different strain, weak strain, diseased plant, before female parent is weaved silk, in time maternal tassel is all pulled out, waited to pollinate after the end, in time male parent is cut off, the maternal fruit ear in maternal ripe back is gathered in the crops in good time, carries out nature and dries or implements after the artificial drying threshing and put in storage and carry out safe storage.
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| CNA2008100122183A CN101313664A (en) | 2008-07-07 | 2008-07-07 | Process for preparing corn hybrid Liangyu 88 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105794629A (en) * | 2016-03-24 | 2016-07-27 | 甘肃省敦煌种业股份有限公司研究院 | Production method of hybrid corn seeds |
| CN106319037A (en) * | 2015-07-10 | 2017-01-11 | 沈阳化工大学 | SSR molecular labeling kit for detecting purity of maize variety Liangyu 88# |
| CN112136684A (en) * | 2020-09-21 | 2020-12-29 | 连云港市农业科学院 | Efficient testing and matching method for corn line selection |
| CN113994885A (en) * | 2021-11-17 | 2022-02-01 | 安徽荃银高科种业股份有限公司 | Method for seed production, cost saving, impurity removal, castration and purity preservation of hybrid corn |
-
2008
- 2008-07-07 CN CNA2008100122183A patent/CN101313664A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106319037A (en) * | 2015-07-10 | 2017-01-11 | 沈阳化工大学 | SSR molecular labeling kit for detecting purity of maize variety Liangyu 88# |
| CN105794629A (en) * | 2016-03-24 | 2016-07-27 | 甘肃省敦煌种业股份有限公司研究院 | Production method of hybrid corn seeds |
| CN112136684A (en) * | 2020-09-21 | 2020-12-29 | 连云港市农业科学院 | Efficient testing and matching method for corn line selection |
| CN113994885A (en) * | 2021-11-17 | 2022-02-01 | 安徽荃银高科种业股份有限公司 | Method for seed production, cost saving, impurity removal, castration and purity preservation of hybrid corn |
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Open date: 20081203 |