CN101233868A - Pueraria peduncularis extract for preventing and controlling plant disease - Google Patents
Pueraria peduncularis extract for preventing and controlling plant disease Download PDFInfo
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- CN101233868A CN101233868A CNA2008100581428A CN200810058142A CN101233868A CN 101233868 A CN101233868 A CN 101233868A CN A2008100581428 A CNA2008100581428 A CN A2008100581428A CN 200810058142 A CN200810058142 A CN 200810058142A CN 101233868 A CN101233868 A CN 101233868A
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- plant disease
- bitter pueraria
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Abstract
The invention relates to pueraria peduncularis extract used for preventing and treating plant diseases. Root, stem or leaf of the pueraria peduncularis are taken as raw materials and are dried, smashed and extracted to obtain the peduncularis used for preventing and treating the plant diseases. The pueraria peduncularis extract of the invention is used for preventing and treating the plant diseases with strong prevention and treatment effects and has effect on diversified plant diseases. As the extract adopts the pueraria peduncularis as the raw material, the extract can be degraded successfully in nature without residue and environment pollution; meanwhile, pathogenic bacteria are not easy to generate drug resistance so that the pueraria peduncularis extract is safe for the non-target organism and is environment friendly.
Description
Technical field
The invention belongs to pesticide field, relate in particular to bitter pueraria root extract and be used for control of plant disease.
Background technology
Along with the development of society, the raising of people's living standard, pattern of farming has had very big adjustment, and planting patterns has had very big change.Vegetables, fruit cultivated area sharply increase; Planting patterns is sparsely developed to short and small intensive direction by tall and big; The greenhouse is widely applied; Cropping index improves greatly.These all provide advantageous conditions for the generation development of disease.The generation of disease is more and more serious, has had a strong impact on the yield and quality of crops.Simultaneously, existing many bactericidal agent for preventing and treating effects are still very undesirable, and many diseases still can not get effective control, and, the traditional chemical bactericide can not adapt to the development of society owing to have contaminated environment,, easy various disadvantages such as develop immunity to drugs harmful to beneficial organism.New and effective, low toxicity, low-residual, receive much attention with the research of eco-friendly natural source bactericide.
Plant and germ have passed through long-term coevolution, with the process of germ struggle in, formed the effective mechanism that a cover prevents germ harm.Plant produce to suppress the material of germ growth, is plant to one of important defence measure of germ harm.These materials have its degradation pathway smoothly at natural world, are difficult for causing residue problem, can not cause environmental pollution; Simultaneously, pathogen is had multiple action target and unique mechanism of action, pathogen is difficult for developing immunity to drugs, to non-target organism safety; Content is low in plant corpus, generally pathogen is had very strong control action.According to these compounds, can develop efficient, low toxicity, wide spectrum, be difficult for producing resistance, with eco-friendly new type bactericide.The vegetalitas bactericide is an important directions of bactericide research and development, is to solve the important measures that current agricultural chemicals causes a series of problems.
Summary of the invention
The invention provides bitter pueraria root extract and be used for control of plant disease, overcome the deficiency that traditional agricultural chemicals is used for control of plant disease.
Bitter pueraria root extract is used for control of plant disease, the application of promptly bitter pueraria root extract in control of plant disease.
The extract of root, stem or leaf that described bitter pueraria root extract is bitter Pueraria lobota.
Described bitter pueraria root extract obtains according to following steps:
(1) root, stem or the leaf of the bitter Pueraria lobota of collection dry in the shade, and place 10 ℃---and 100 ℃ of drying boxes are dry, pulverize with cracker then, get bitter kudzuvine root starch;
(2)---solvent of 30 times of volumes is at 10 ℃---100 ℃ are adopted specific extracting method to extract down 1 minute---30 days with 1 with bitter kudzuvine root starch;
(3) extract after will filtering is at 10 ℃---and 100 ℃ concentrate down, promptly get bitter pueraria root extract.
Described extracting method is soxhlet extraction, extraction, ultrasonic extraction, decocting method or percolation.
Described solvent is methyl alcohol, ethanol, chloroform, acetone or water.
The invention has the beneficial effects as follows: bitter pueraria root extract provided by the present invention is used for control of plant disease, has stronger preventive and therapeutic effect, and plurality of plant diseases there is effect, because of it is a raw material with the bitter Pueraria lobota of plant, at natural world its degradation pathway is smoothly arranged, be difficult for causing residue problem, can not cause environmental pollution; Simultaneously, pathogen is difficult for developing immunity to drugs, to non-target organism safety, with environmental friendliness.
Embodiment
Embodiment one:
Gather bitter Ge Ye, dry in the shade, place 10 ℃ of drying boxes dry, cracker is pulverized.Gained Eupatorium adenophorum powder extracted 30 days with extraction under 10 ℃ with the methyl alcohol of 1 times of volume.Extract after filtering is concentrated down at 60 ℃, promptly get bitter Ge Ye methanolic extract.
Water is mixed with soup to bitter Ge Ye methanolic extract, at 2000g/hm
2Consumption under, to being respectively 62.3%, 83.5%, 80.2%, 85.6 and 77.3% in 10 days behind the control efficiency medicine of rice sheath blight disease, pear scab, wheat powdery mildew, bacterial wilt of peanut, tobacco mosaic.
Embodiment two:
Gather omei mountain kudzuvine root, dry in the shade, place 50 ℃ of drying boxes dry, cracker is pulverized.Gained Eupatorium adenophorum powder extracted 5 days with soxhlet extraction under 70 ℃ with the chloroform of 5 times of volumes.Extract after filtering is concentrated down at 50 ℃, promptly get omei mountain kudzuvine root chloroform extract.
Water is mixed with soup to omei mountain kudzuvine root chloroform extract, at 2000g/hm
2Consumption under, to being respectively 73.5%, 79.5%, 76.4%, 83.7 and 72.6% in 10 days behind the control efficiency medicine of rice sheath blight disease, pear scab, wheat powdery mildew, bacterial wilt of peanut, tobacco mosaic.
Embodiment three:
Gather bitter Pueraria lobota stem, dry in the shade, place 100 ℃ of drying boxes dry, cracker is pulverized.Gained Eupatorium adenophorum powder extracted 1 minute with ultrasonic extraction under 25 ℃ with 3 times of volume of ethanol.Extract after filtering is concentrated down at 60 ℃, promptly get bitter Pueraria lobota stem ethanol extract.
Water is mixed with soup to bitter Pueraria lobota stem ethanol extract, at 2000g/hm
2Consumption under, to being respectively 67.5%, 80.2%, 78.4%, 84.5 and 73.56% in 10 days behind the control efficiency medicine of rice sheath blight disease, pear scab, wheat powdery mildew, bacterial wilt of peanut, tobacco mosaic.
Embodiment four:
Gather omei mountain kudzuvine root, dry in the shade, place 50 ℃ of drying boxes dry, cracker is pulverized.Gained Eupatorium adenophorum powder extracted 30 minutes with decocting method under 100 ℃ with the water of 30 times of volumes.Extract after filtering is concentrated down at 100 ℃, promptly get the omei mountain kudzuvine root water extract.
Water is mixed with soup to the omei mountain kudzuvine root water extract, at 2000g/hm
2Consumption under, to being respectively 77.6%, 78.1%, 776.8%, 86.0 and 79.5% in 10 days behind the control efficiency medicine of rice sheath blight disease, pear scab, wheat powdery mildew, bacterial wilt of peanut, tobacco mosaic.
Embodiment five:
Gather omei mountain kudzuvine root, dry in the shade, place 50 ℃ of drying boxes dry, cracker is pulverized.Gained Eupatorium adenophorum powder is a solvent with acetone, extracts 7 days with percolation under the normal temperature.Extract after filtering is concentrated down at 50 ℃, promptly get the omei mountain kudzuvine root acetone extract.
Water is mixed with soup to the omei mountain kudzuvine root acetone extract, at 2000g/hm
2Consumption under, to being respectively 74.2%, 85.8%, 75.8%, 84.7 and 76.7% in 10 days behind the control efficiency medicine of rice sheath blight disease, pear scab, wheat powdery mildew, bacterial wilt of peanut, tobacco mosaic.
The bitter pueraria root extract of table 1 is to the preventive and therapeutic effect of rice sheath blight disease and early blight of tomato
| Handle | Control efficiency (%) to rice sheath blight disease | Control efficiency (%) to pear scab | Control efficiency (%) to wheat powdery mildew | Control efficiency (%) to bacterial wilt of peanut | Control efficiency (%) to tobacco mosaic |
| Bitter Ge Ye methanolic extract 2000g/hm 2Omei mountain kudzuvine root chloroform extract 2000g/hm 2Bitter Pueraria lobota stem ethanol extract 2000g/hm 2Omei mountain kudzuvine root water extract 2000g/hm 2Omei mountain kudzuvine root acetone extract 2000g/hm 2Jinggangmeisu 750g/hm 2Flusilazole 80g/hm 2Triazolone 100g/hm 2Captan 1950g/hm 2Virus must restrain 500g/hm 2 | 62.3 73.5 67.5 77.6 74.2 75.8 - - - - | 83.5 79.5 80.2 78.1 85.8 - 92.6 - - - | 80.2 76.4 78.4 76.8 75.8 - - 87.9 - - | 85.6 83.7 84.5 86.0 84.7 - - - 94.8 - | 77.3 72.6 73.5 79.5 76.7 - - - - 83.2 |
Claims (5)
1, bitter pueraria root extract is used for control of plant disease, it is characterized in that the application of bitter pueraria root extract in control of plant disease.
2, bitter pueraria root extract according to claim 1 is used for control of plant disease, it is characterized in that described bitter pueraria root extract is the extract of the root of bitter Pueraria lobota, stem or leaf.
3, bitter pueraria root extract according to claim 1 is used for control of plant disease, it is characterized in that described bitter pueraria root extract obtains according to following steps:
(1) root, stem or the leaf of the bitter Pueraria lobota of collection dry in the shade, and place 10 ℃---and 100 ℃ of drying boxes are dry, pulverize with cracker then, get bitter kudzuvine root starch;
(2)---solvent of 30 times of volumes is at 10 ℃---100 ℃ are adopted specific extracting method to extract down 1 minute---30 days with 1 with bitter kudzuvine root starch;
(3) extract after will filtering is at 10 ℃---and 100 ℃ concentrate down, promptly get bitter pueraria root extract.
4, bitter pueraria root extract according to claim 3 is used for control of plant disease, it is characterized in that described extracting method is soxhlet extraction, extraction, ultrasonic extraction, decocting method or percolation.
5, bitter pueraria root extract according to claim 3 is used for control of plant disease, it is characterized in that described solvent is methyl alcohol, ethanol, chloroform, acetone or water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100581428A CN101233868A (en) | 2008-03-04 | 2008-03-04 | Pueraria peduncularis extract for preventing and controlling plant disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100581428A CN101233868A (en) | 2008-03-04 | 2008-03-04 | Pueraria peduncularis extract for preventing and controlling plant disease |
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| Publication Number | Publication Date |
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| CN101233868A true CN101233868A (en) | 2008-08-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2008100581428A Pending CN101233868A (en) | 2008-03-04 | 2008-03-04 | Pueraria peduncularis extract for preventing and controlling plant disease |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106857696A (en) * | 2017-04-11 | 2017-06-20 | 四川农业大学 | A kind of Pesticidal combination containing bitter pueraria root extract and sanguinarine and application thereof |
| CN106857697A (en) * | 2017-04-11 | 2017-06-20 | 四川农业大学 | A kind of Pesticidal combination containing bitter pueraria root extract and jervine and application thereof |
| CN106900756A (en) * | 2017-04-11 | 2017-06-30 | 四川农业大学 | Insecticidal composition of pueraria peduncularis extract and azadirachtin for preventing and treating agricultural pests and application thereof |
| CN106900757A (en) * | 2017-04-11 | 2017-06-30 | 四川农业大学 | A kind of Pesticidal combination of the bitter pueraria root extract for preventing and treating agricultural pests and Osthole and application thereof |
| CN106973949A (en) * | 2017-04-11 | 2017-07-25 | 四川农业大学 | A kind of Pesticidal combination comprising bitter pueraria root extract and matrine and application thereof |
| CN108041084A (en) * | 2017-12-28 | 2018-05-18 | 成都新朝阳作物科学有限公司 | The prevention mushroom pesticide and its production method produced with bitter Pueraria lobota and purple a species of orchid Chinese scholartree |
-
2008
- 2008-03-04 CN CNA2008100581428A patent/CN101233868A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106857696A (en) * | 2017-04-11 | 2017-06-20 | 四川农业大学 | A kind of Pesticidal combination containing bitter pueraria root extract and sanguinarine and application thereof |
| CN106857697A (en) * | 2017-04-11 | 2017-06-20 | 四川农业大学 | A kind of Pesticidal combination containing bitter pueraria root extract and jervine and application thereof |
| CN106900756A (en) * | 2017-04-11 | 2017-06-30 | 四川农业大学 | Insecticidal composition of pueraria peduncularis extract and azadirachtin for preventing and treating agricultural pests and application thereof |
| CN106900757A (en) * | 2017-04-11 | 2017-06-30 | 四川农业大学 | A kind of Pesticidal combination of the bitter pueraria root extract for preventing and treating agricultural pests and Osthole and application thereof |
| CN106973949A (en) * | 2017-04-11 | 2017-07-25 | 四川农业大学 | A kind of Pesticidal combination comprising bitter pueraria root extract and matrine and application thereof |
| CN106973949B (en) * | 2017-04-11 | 2019-06-11 | 四川农业大学 | A kind of Pesticidal combination and application thereof comprising bitter pueraria root extract and matrine |
| CN106857697B (en) * | 2017-04-11 | 2019-06-11 | 四川农业大学 | A kind of Pesticidal combination and application thereof containing bitter pueraria root extract and jervine |
| CN106900756B (en) * | 2017-04-11 | 2019-06-11 | 四川农业大学 | Insecticidal composition of pueraria peduncularis extract and azadirachtin for preventing and treating agricultural pests and application thereof |
| CN106857696B (en) * | 2017-04-11 | 2019-06-11 | 四川农业大学 | A kind of Pesticidal combination and application thereof containing bitter pueraria root extract and sanguinarine |
| CN106900757B (en) * | 2017-04-11 | 2019-09-06 | 四川农业大学 | A kind of bitter pueraria root extract and the Pesticidal combination of Osthole and application thereof for preventing and treating agricultural pests |
| CN108041084A (en) * | 2017-12-28 | 2018-05-18 | 成都新朝阳作物科学有限公司 | The prevention mushroom pesticide and its production method produced with bitter Pueraria lobota and purple a species of orchid Chinese scholartree |
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Open date: 20080806 |