CN106857697B - A kind of Pesticidal combination and application thereof containing bitter pueraria root extract and jervine - Google Patents
A kind of Pesticidal combination and application thereof containing bitter pueraria root extract and jervine Download PDFInfo
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- CN106857697B CN106857697B CN201710233208.1A CN201710233208A CN106857697B CN 106857697 B CN106857697 B CN 106857697B CN 201710233208 A CN201710233208 A CN 201710233208A CN 106857697 B CN106857697 B CN 106857697B
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- pesticidal combination
- jervine
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- root extract
- pueraria root
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- 239000000284 extract Substances 0.000 title claims abstract description 53
- 241000219780 Pueraria Species 0.000 title claims abstract description 52
- CLEXYFLHGFJONT-DNMILWOZSA-N Jervine Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H](C(=O)C2=C3C)[C@@H]1[C@@H]2CC[C@@]13O[C@@H]2C[C@H](C)CN[C@H]2[C@H]1C CLEXYFLHGFJONT-DNMILWOZSA-N 0.000 title claims abstract description 38
- CLEXYFLHGFJONT-UHFFFAOYSA-N jervine Natural products C1C=C2CC(O)CCC2(C)C(C(=O)C2=C3C)C1C2CCC13OC2CC(C)CNC2C1C CLEXYFLHGFJONT-UHFFFAOYSA-N 0.000 title claims abstract description 38
- QRXOCOSLDOPPKH-UHFFFAOYSA-N jervine sulfate Natural products CC1CNC2C(C1)OC3(CCC4=C(C3C)C(=O)C5C4CC=C6CC(O)CCC56C)C2C QRXOCOSLDOPPKH-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 230000000361 pesticidal effect Effects 0.000 title claims abstract description 20
- 239000004530 micro-emulsion Substances 0.000 claims abstract description 22
- 241001151957 Aphis aurantii Species 0.000 claims abstract description 16
- 241001477931 Mythimna unipuncta Species 0.000 claims abstract description 14
- 240000008042 Zea mays Species 0.000 claims abstract description 14
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 14
- 241000500437 Plutella xylostella Species 0.000 claims abstract description 12
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 11
- 240000007124 Brassica oleracea Species 0.000 claims abstract description 10
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims abstract description 10
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims abstract description 10
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims abstract description 10
- 241000255972 Pieris <butterfly> Species 0.000 claims abstract description 10
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims abstract description 10
- 235000009973 maize Nutrition 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 239000000470 constituent Substances 0.000 claims abstract description 8
- 241000086608 Empoasca vitis Species 0.000 claims abstract description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 42
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical group OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 33
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 14
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 14
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 14
- 230000002265 prevention Effects 0.000 claims description 13
- 239000012141 concentrate Substances 0.000 claims description 6
- 238000002386 leaching Methods 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- 239000003995 emulsifying agent Substances 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- -1 polyoxyethylene Polymers 0.000 claims description 4
- 239000012752 auxiliary agent Substances 0.000 claims description 3
- 239000006184 cosolvent Substances 0.000 claims description 3
- 239000003480 eluent Substances 0.000 claims description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 3
- 239000012046 mixed solvent Substances 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims 1
- 241000607479 Yersinia pestis Species 0.000 abstract description 19
- 239000002917 insecticide Substances 0.000 abstract description 11
- 230000002195 synergetic effect Effects 0.000 abstract description 5
- 230000002829 reductive effect Effects 0.000 abstract description 4
- 231100000053 low toxicity Toxicity 0.000 abstract description 2
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- 230000001988 toxicity Effects 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 241000409991 Mythimna separata Species 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 241001046947 Ectropis obliqua Species 0.000 description 4
- 241000255969 Pieris brassicae Species 0.000 description 4
- 244000046146 Pueraria lobata Species 0.000 description 4
- 235000010575 Pueraria lobata Nutrition 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 3
- 240000003553 Leptospermum scoparium Species 0.000 description 3
- 235000015459 Lycium barbarum Nutrition 0.000 description 3
- 239000002552 dosage form Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000000855 fungicidal effect Effects 0.000 description 3
- 239000000417 fungicide Substances 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000004550 soluble concentrate Substances 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 230000009471 action Effects 0.000 description 2
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
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- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000008177 pharmaceutical agent Substances 0.000 description 2
- 239000002574 poison Substances 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
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- 238000001228 spectrum Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 241000198596 Alternaria tomatophila Species 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241000931705 Cicada Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000578422 Graphosoma lineatum Species 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 241000500441 Plutellidae Species 0.000 description 1
- 208000001431 Psychomotor Agitation Diseases 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- 241000167882 Rhopalosiphum maidis Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 241001163248 Schoenocaulon officinale Species 0.000 description 1
- 241001414989 Thysanoptera Species 0.000 description 1
- 241000489523 Veratrum Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000013473 artificial intelligence Methods 0.000 description 1
- 239000002021 butanolic extract Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 description 1
- 150000004657 carbamic acid derivatives Chemical class 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- RECVMTHOQWMYFX-UHFFFAOYSA-N oxygen(1+) dihydride Chemical compound [OH2+] RECVMTHOQWMYFX-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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- 239000007921 spray Substances 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
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- 235000013311 vegetables Nutrition 0.000 description 1
- 229940057613 veratrum Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of Pesticidal combination, the active constituent in the Pesticidal combination is the composition that weight ratio is 1:6~6:1 hardship pueraria root extract and jervine, and the Pesticidal combination is further configured to microemulsion.Pesticidal combination of the invention compared to single agent for, have certain synergistic effect, while there is low cost, low toxicity, the advantages that pest resistance to insecticide can be reduced, and the agricultural pests such as cabbage pieris, diamondback moth, maize army worm, tea aphid, smaller green leaf hopper can be effectively prevented.
Description
Technical field
The invention belongs to technical field of pesticide, it is related to a kind of for preventing and treating the pharmaceutical agent combinations of agricultural pests, relates more specifically to
Pharmaceutical agent combinations are configured to microemulsion, for the agricultural evil such as maize army worm, cabbage pieris, diamondback moth, tea aphid, tea lesser leafhopper
The prevention and treatment of worm.
Technical background
Diseases and pests of agronomic crop is one of the Main Agricultural disaster in China, and, influence more with type is greatly and outburst often is caused disaster
The characteristics of, occurrence scope and severity often result in heavy losses to Chinese national economy, particularly agricultural production.As tea is small
Greenery cicada can seriously cause harm Summer-autumn tea, aggrieved tea tree bud-leaf rolls up, hardens, just, blade tip and leaf margin it is reddish brown dried-up, the growth of the bud tip is slow
Slowly, very big to tea yield and qualitative effects.
Agricultural pests can be prevented and treated using the methods of physics, chemistry, biology and agricultural in production at present, wherein chemistry kills
Worm agent have efficiently, quickly, commercialized degree is high, can apply immediately after kind and dosage form multiplicity, purchase in field, method of administration
The advantages that easy, becomes the measure that most pest controls is applied in production.Including organophosphorus insecticides, carbamates
Insecticide, nicotinic insecticide etc., play the important and pivotal role in agricultural insect pests control.But chemical insecticide has wide spectrum
Property, easily kill off the insect pests natural enemy while killing off the insect pests, and is also easy to produce the rampant and minor pests again to kill off the insect pests and rises to Main Harmful
Worm.The use of chemical insecticide also easily causes pollution problem.Therefore, low toxicity, low-residual, degradable plant insecticide etc. are raw
Object insecticide can make up the deficiency of chemical pesticide.
Bitter Pueraria lobota category pulse family Pueraria lobota category, alias Yunnan kudzu are distributed widely in the ground such as China Tibet, Yunnan, Sichuan, Guangxi, Pueraria lobota
The pharmacological activity of platymiscium is very strong, omei mountain kudzuvine root energy desinsection, and flower can relieve the effect of alcohol, relieving restlessness heat.Wang Jing et al. presses down to bitter pueraria root extract
Find bitter pueraria root extract to rice sheath blight disease, inner scab, oat reaping hook germ, early blight of tomato in the active Primary Study of bacterium
The mycelia growth of bacterium, tobacco brown spot pathogen has very strong inhibiting effect, and with the raising of concentration, inhibiting effect enhancing；
Shi Rui et al. has found that bitter Pueraria lobota n-butanol extract hangs pipe to corn in insecticidal activity research of the bitter kudzu Chenopodiaceae to maize aphids
Aphid has very strong contact toxicity, LD50Value is 14.55mg/L.Jervine is to be present in Liliaceae Veratrum and sabadilla category plant
Insecticide active substance in object has insect and tags and stomach poison function.Can be used for preventing and treating cabbage caterpillar, aphid, leafhopper, thrips and
The agricultural pests such as stinkbug.Jervine is pure plant source preparation, no drug resistance.By the strong bitter pueraria root extract of quick-acting and holding effect
The phase broad spectrum pesticide jervine of length is compounded, and vegetable insecticide can be improved to the effect of control of insect.Currently, not yet sending out
Now bitter pueraria root extract is carried out to compound the relevant report for being used for agricultural insect pests control with jervine.
Summary of the invention
The present invention provides the complex composition of bitter pueraria root extract and jervine, and the two has synergistic effect, further, will
The two is prepared into microemulsion, the agricultures such as prevention and treatment cabbage pieris, diamondback moth, maize army worm, tea tree tea geometrid, tea aphid, smaller green leaf hopper
Industry pest.
Based on this purpose, the technical solution of the present invention is as follows:
A kind of Pesticidal combination, which is characterized in that it is 1:6~6 that the active constituent in the Pesticidal combination, which is weight ratio:
The composition of 1 bitter pueraria root extract and jervine.
Preferably, the weight ratio of the two is 1:3~3:1；It is highly preferred that the weight ratio of the two is 1:3.
Further, the present invention also provides a kind of microemulsions, which is characterized in that it includes above-mentioned Pesticidal combination and helps
Agent；Wherein the dosage of Pesticidal combination is 1~20 weight %, and auxiliary agent is 20~40 weight % of solvent, 10~25 weight of emulsifier
% is measured, 5~15 weight % of cosolvent, 3~5 weight % of antifreezing agent, remaining supplies 100 weight % with water.
The solvent is methanol.
The emulsifier is styryl phenyl polyoxyethylene ether, alkyl phenol polyoxyethylene ether, polyaryl phenol polyethenoxy
The combination of one or more of ether phosphate.
The cosolvent is isopropanol, one kind of n-butanol or mixture.
The antifreezing agent is ethylene glycol.
Preferably, the dosage of effective component is 4~16 weight %；It is highly preferred that the dosage of effective component is 10 weight %；
Further, the present invention provides above-mentioned Pesticidal combination and/or microemulsion prevents and treats cabbage pieris, diamondback moth, jade
The purposes of rice mythimna separata, tea aphid or smaller green leaf hopper.
The bitter pueraria root extract is dried in 80 DEG C of baking ovens the preparation method comprises the following steps: the root of bitter Pueraria lobota to be cut into the segment of l-2cm long
It is dry, it crushed 50 meshes, collect spare.Refluxing extraction is carried out at 70 DEG C using 75% ethanol water, each 8h extracts 3
It is secondary, leaching liquor is collected, residue is filtered to remove, leaching liquor is concentrated under reduced pressure, collection obtains dry medicinal extract.Medicinal extract is diluted with water,
With extracting n-butyl alcohol, the ratio of extractant and concentrate aqueous is 4:1, and extract liquor is concentrated and dried, concentrate AB-8 resin
Static Adsorption is stayed overnight.The mixed solvent elution for being 3:7 with water and methanol ratio, eluent is concentrated and dried, and as bitter Pueraria lobota is extracted
Object.
Unless otherwise indicated, " % " in this specification is " weight percent ".
The preparation method of the application microemulsion: each components in certain proportion measured is put into emulsification cutter；High speed is cut
It cuts 30 minutes, the product can be obtained, used by spraying after dilution.
Microemulsion is a kind of novel environment friendly dosage form of water baseization, and powder is fine, is easy to permeate insect body wall, thus to nocuousness
Insect plays better toxic action.Effective component used in microemulsion of the invention is plant medical agent, to non-target organism and ring
Border safety, to person poultry harmless, is suitble to use in various crop, and it is viscous to effectively prevent cabbage pieris, diamondback moth, corn
The agricultural pests such as worm, tea tree tea geometrid, tea aphid, smaller green leaf hopper.Meanwhile using mixed dosage form, compared to single agent for, insecticidal spectrum
Extensively, there is synergistic effect, and use cost can be effectively reduced, reduce pest resistance to insecticide.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention with following specific example into
Row explanation, but the present invention is not limited to these specific embodiments.
The root of bitter Pueraria lobota: being cut into the segment of l-2cm long by bitter pueraria root extract preparation method, is dried in 80 DEG C of baking ovens, is crushed
50 meshes are crossed, are collected spare.Refluxing extraction is carried out at 70 DEG C using 75% ethanol water, each 8h is extracted 3 times, is collected
Leaching liquor is filtered to remove residue, and leaching liquor is concentrated under reduced pressure, and collection obtains dry medicinal extract.Medicinal extract is diluted with water, n-butanol is used
The ratio of extraction, extractant and concentrate aqueous is 4:1, and extract liquor is concentrated and dried, concentrate AB-8 resin Static Adsorption
Overnight.The mixed solvent elution for being 3:7 with water and methanol ratio, eluent is concentrated and dried, as bitter pueraria root extract.
One, embodiment is prepared
Embodiment 1:
Bitter pueraria root extract 1.43wt%；Jervine 8.57wt%；Methanol 30wt%, styryl phenyl polyoxyethylene ether
10wt%；Isopropanol 10wt%；Ethylene glycol 3wt%；Surplus is supplied with water.
Embodiment 2:
Bitter pueraria root extract 2.5wt%；Jervine 7.5wt%；Methanol 30wt%, styryl phenyl polyoxyethylene ether
10wt%；Isopropanol 10wt%；Ethylene glycol 3wt%；Surplus is supplied with water.
Embodiment 3:
Bitter pueraria root extract 5wt%；Jervine 5wt%；Methanol 30wt%, styryl phenyl polyoxyethylene ether 10wt%；
Isopropanol 10wt%；Ethylene glycol 3wt%；Surplus is supplied with water.
Embodiment 4:
Bitter pueraria root extract 7.5wt%；Jervine 2.5wt%；Methanol 30wt%, styryl phenyl polyoxyethylene ether
10wt%；Isopropanol 10wt%；Ethylene glycol 3wt%；Surplus is supplied with water.
Embodiment 5:
Bitter pueraria root extract 8.57wt%；Jervine 1.43wt%；Methanol 30wt%, styryl phenyl polyoxyethylene ether
10wt%；Isopropanol 10wt%；Ethylene glycol 3wt%；Surplus is supplied with water.
Embodiment 6:
Bitter pueraria root extract 1wt%；Jervine 3wt%；Methanol 30wt%, styryl phenyl polyoxyethylene ether 10wt%；
Isopropanol 10wt%；Ethylene glycol 3wt%；Surplus is supplied with water.
Embodiment 7:
Bitter pueraria root extract 2wt%；Jervine 6wt%；Methanol 30wt%, styryl phenyl polyoxyethylene ether 10wt%；
Isopropanol 10wt%；Ethylene glycol 3wt%；Surplus is supplied with water.
Embodiment 8:
Bitter pueraria root extract 3wt%；Jervine 9wt%；Methanol 30wt%, styryl phenyl polyoxyethylene ether 10wt%；
Isopropanol 10wt%；Ethylene glycol 3wt%；Surplus is supplied with water.
Embodiment 9:
Bitter pueraria root extract 4wt%；Jervine 12wt%；Methanol 30wt%, styryl phenyl polyoxyethylene ether 10wt%；
Isopropanol 10wt%；Ethylene glycol 3wt%；Surplus is supplied with water.
Two, biological Examples
1, indoor bactericidal activity measures
1.1 Study on Fungicide Toxicity
Referring to farm-chemical indoor determination test rule, using dip method measurement for examination single dose to the poison of 3 age armyworm larvaes
Kill activity.Bitter pueraria root extract and jervine are respectively set into 5 concentration for the treatment of (being diluted with dimethyl sulfoxide solvent).Take 3 age of mythimna separata young
Worm impregnates 10s in each concentration liquid, takes out, blots extra medical fluid with blotting paper, be placed in the clean container for being placed with fresh food
In.Dimethyl sulfoxide compares.Each concentration handles 20, is repeated 4 times.Then processed test worm is put into artificial intelligence gas
Wait raising in case culture (28 DEG C ± 1 DEG C, RH=80%).Number of dead and live insects is checked after 72h, cannot normally creep as death.Meter
The insect population death rate, corrected mortality are calculated, and finds out by probit analysis method the toxicity regression curve and LC of two single doses50Value.
The complex role research of 1.2 bitter pueraria root extract objects and jervine
Bitter pueraria root extract and jervine are compounded: two kinds of raw medicines are configured to 6:1,3:1,1:1,1 by effective component:
3,1:6 totally 5 proportions (mass ratio).Above-mentioned formula presses Study on Fungicide Toxicity method respectively and carries out toxicity test.
Mixture synergy is evaluated, with LC50Based on value, mixture joint is evaluated by calculating mixture co-toxicity coefficient
Type of action.
CTC>=120 are synergistic effect, and 80≤CTC<120 are summation action, and CTC<80 are antagonism.
2. test result
2.1 Study on Fungicide Toxicity results
The Toxicity Determination result of the 12 kinds of medicaments of table to mythimna separata
As it can be seen from table 1 LC of the bitter Pueraria lobota crude extract to 3 age armyworm larvaes50For 257.7427 μ g/mL；Jervine is to 3 ages
The LC of armyworm larvae50For 112.9140 μ g/mL.
The bitter pueraria root extract of table 2 compounds the The Toxicity Determination result to 3 age armyworm larvaes with jervine
As shown in Table 2, bitter pueraria root extract and all proportions of jervine compounding show certain synergistic effect, but not
With compound proportion coefficient of synergism difference.Wherein, when the compound proportion of bitter Pueraria lobota crude extract and jervine is 1:3, co-toxicity coefficient
Highest is 221.18.
Two, field experiment
1. test method
Cabbage caterpillar and diamondback moth test are carried out in Chengdu double fluid area；Maize army worm test is carried out in Renshou County；Tea geometrid
Carry out with the test of tea aphid in Ya'an Mingshan County.Experimental field soil types is clay, and soil fertility is medium, cell cultivation condition one
It causes.Plot area 20m2, centre setting isolation strip.It is each to implement in cabbage caterpillar, diamondback moth, mythimna separata and tea geometrid controlling experiment
Example presses effective component 20g/hm2Dosage it is spraying against plant, medicament control is 0.5% jervine soluble concentrate and 10%
Bitter pueraria root extract microemulsion, dosage are effective component 20g/hm2；In tea aphid, tea lesser leafhopper controlling experiment, each embodiment
By effective component 2g/hm2Dosage it is spraying against plant, medicament control is that 0.5% jervine soluble concentrate and 10% are bitter
Pueraria root extract microemulsion, dosage are effective component 2g/hm2；, to spray clear water as blank control, test every processing and repeat 4
Secondary, random district's groups carry out cell arrangement.1d, 3d, 7d and 14d investigate the borer population in each cell after application, according to clear water check plot
Control efficiency is calculated with the borer population for the treatment of region, and carries out significance difference analysis.
In formula: TaThe individual amount survived after the prevention and treatment of-treatment region；TbThe individual amount survived before the prevention and treatment of-treatment region；
CaThe individual amount survived after the prevention and treatment of-check plot；CbThe individual amount survived before the prevention and treatment of-check plot.
The preparation of 10% bitter pueraria root extract microemulsion: weighing omei mountain kudzuvine root extract 10kg, be dissolved in 20kg n-butanol, then
Alkyl phenol polyoxyethylene ether 5kg, calcium dodecyl benzene sulfonate 15kg is added, is mixed in the case where temperature is 45 DEG C of stirring condition；?
While stirring, water ion water 50kg is slowly added dropwise, continues to stir 1h after completion of dropwise addition to get to 10% bitter pueraria root extract micro emulsion
Agent 100kg.Resulting preparation meets national microemulsion performance indicator requirement on the Testing index such as stability, appearance.
2. test result
9 complex microemulsions of bitter pueraria root extract and jervine are determined to maize army worm, cabbage pieris, diamondback moth, tea
Aphid, tea lesser leafhopper field control effect, be shown in Table 3, table 4, table 5, table 6 and table 7.
As can be seen that different embodiments are equal to maize army worm, cabbage pieris and diamondback moth from 5 table of table 3, table 4 and table
There is different degrees of preventive and therapeutic effect.Wherein, when bitter pueraria root extract and jervine effective component are 10wt%, matching in the two is 1:
(embodiment 2, best to the above pest controling effect, being higher than other proportions, (embodiment 1, embodiment 3, embodiment 4 are implemented when 3
Example 5 and comparison medicament processing).When active constituent content is lower than 10wt% (embodiment 6 and embodiment 7), to maize army worm etc.
The control efficiency of insect pest is decreased obviously；It is viscous to corn when active constituent content is higher than 10wt% (embodiment 8 and embodiment 9)
The control efficiency of the insect pests such as worm is without significantly improving.It can thus be seen that when bitter pueraria root extract and jervine effective component are
10wt%, and it is best available component content and proportion that the two proportion, which is 1:3, to Corn mythimna separata, cabbage pieris, diamondback moth
There is good control efficiency.
From 7 table of table 6 and table data can be seen that different embodiments have to tea aphid, tea lesser leafhopper it is different degrees of
Preventive and therapeutic effect, effect is obviously higher than 0.5% jervine soluble concentrate of comparison medicament and 10% bitter pueraria root extract microemulsion.Its
In, when bitter pueraria root extract and jervine effective component are 10wt%, when the two proportion is 1:3 (embodiment 2), to tea aphid, tea
Smaller green leaf hopper control efficiency is best, is higher than other proportions (embodiment 1, embodiment 3, embodiment 4, at embodiment 5 and comparison medicament
Reason).When active constituent content is lower than 10wt% (embodiment 6 and embodiment 7), to the control efficiency of tea aphid, tea lesser leafhopper
It is remarkably decreased；Prevention and treatment when active constituent content is higher than 10wt% (embodiment 8 and embodiment 9), to tea aphid, tea lesser leafhopper
Effect is also significantly lower than embodiment 2.
It can thus be seen that when bitter pueraria root extract and jervine effective component are 10wt%, and it is most that the two proportion, which is 1:3,
Good active constituent content and proportion have good control efficiency to tea aphid, tea lesser leafhopper.
These results suggest that when bitter pueraria root extract and jervine effective component are 10wt%, when the two proportion is 1:3
(embodiment 2) has good field control effect to maize army worm, cabbage pieris, diamondback moth, tea aphid, tea lesser leafhopper, and
Better than other embodiments (embodiment 1, embodiment 3, embodiment 4, embodiment 5, embodiment 6, embodiment 7, embodiment 8, embodiment
9 and comparison medicament processing).
The field efficacy of the different embodiment processing prevention and treatment maize army worms of table 3
The field efficacy of the different embodiment processing prevention and treatment cabbage caterpillars of table 4
The field efficacy of the different embodiment processing prevention and treatment diamondback moths of table 5
The field efficacy of the different embodiment processing prevention and treatment tea aphids of table 6
The field efficacy of the different embodiment processing prevention and treatment tea lesser leafhoppers of table 7
A kind of Pesticidal combination and application thereof containing bitter pueraria root extract and jervine of the invention has passed through specific real
Example is applied to be described.Those skilled in the art can use for reference the links such as the appropriate feed change of the contents of the present invention, process conditions
To realize that corresponding other purposes, correlation change all without departing from the contents of the present invention, all similar substitutions and modifications pair
It is it will be apparent that being considered as being included within the scope of the present invention for those skilled in the art.
Claims (10)
1. a kind of Pesticidal combination, which is characterized in that the active constituent in the Pesticidal combination is that weight ratio is 1:6~6:1
The composition of bitter pueraria root extract and jervine；
The root of bitter Pueraria lobota is cut into the segment of l-2cm long, is dried in 80 DEG C of baking ovens, crushed 50 meshes, collects spare, use
75% ethanol water carries out refluxing extraction at 70 DEG C, and each 8h is extracted 3 times, collects leaching liquor, is filtered to remove residue, subtracts
Pressure concentration leaching liquor, collection obtains dry medicinal extract, medicinal extract is diluted with water, with extracting n-butyl alcohol, extractant and concentrate
The ratio of aqueous is 4:1, and extract liquor is concentrated and dried, and concentrate is stayed overnight with AB-8 resin Static Adsorption.It is with water and methanol ratio
The mixed solvent of 3:7 elutes, and eluent is concentrated and dried, as bitter pueraria root extract.
2. Pesticidal combination according to claim 1, which is characterized in that the weight ratio of bitter pueraria root extract and jervine is 1:
3~3:1.
3. Pesticidal combination according to claim 2, which is characterized in that the weight ratio of bitter pueraria root extract and jervine is 1:
3。
4. a kind of microemulsion, which is characterized in that it includes the claims 1 to 3 any Pesticidal combinations and auxiliary agent；Wherein
The dosage of Pesticidal combination is 1~20 weight %, and auxiliary agent is 20~40 weight % of solvent, 10~25 weight % of emulsifier, hydrotropy
5~15 weight % of agent, 3~5 weight % of antifreezing agent, remaining supplies 100 weight % with water.
5. microemulsion according to claim 4, which is characterized in that the solvent is methanol.
6. microemulsion according to claim 4, which is characterized in that the emulsifier is styryl phenyl polyoxyethylene
The combination of one or more of ether, alkyl phenol polyoxyethylene ether, polyarylphenol-polyoxyethylene ether phosphate.
7. microemulsion according to claim 4, which is characterized in that the cosolvent be isopropanol, n-butanol one kind or
Mixture.
8. microemulsion according to claim 4, which is characterized in that the antifreezing agent is ethylene glycol.
9. microemulsion according to claim 4, which is characterized in that the dosage of Pesticidal combination is 5~15 weight %.
10. Pesticidal combination according to any one of claims 1 to 3 or any microemulsion prevention and treatment of claim 4 to 9
Cabbage pieris, diamondback moth, maize army worm, tea aphid or smaller green leaf hopper purposes.
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