CN103238639B - Plant fungicide for preventing and treating chill continuous cropping withering disease and preparation method thereof - Google Patents
Plant fungicide for preventing and treating chill continuous cropping withering disease and preparation method thereof Download PDFInfo
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- CN103238639B CN103238639B CN201310200593.1A CN201310200593A CN103238639B CN 103238639 B CN103238639 B CN 103238639B CN 201310200593 A CN201310200593 A CN 201310200593A CN 103238639 B CN103238639 B CN 103238639B
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Abstract
The invention discloses a plant fungicide for preventing and treating chill continuous cropping withering disease and a preparation method of the plant fungicide, and relates to a disease preventing fungicide and a preparation method of the disease preventing fungicide. The plant fungicide comprises the following raw materials in percentage by weight: 40-50wt% of cornstalk, 50-60wt% of water and 100-1,000ml of ethanol. The preparation method comprises the following steps of: weighing the raw materials, crushing the cornstalk, soaking the cornstalk into water, extracting biochemical substances of the cornstalk by using a pressurizing percolation method, absorbing by using an XAD-4 resin column, separating phenolic acid substances, and concentrating an ethanol separating agent of the cornstalk by using a vacuum freezing drying machine so as to obtain an additive for slowing autotoxicity of the chill. The additive is non-toxic, harmless, safe and effective, both the problems of phytotoxicity of chemical medicines and pollution to the environment are avoided, and the chill continuous cropping withering disease is effectively prevented and treated, and meanwhile the additive is simple and convenient to apply when being used for overcoming the chill continuous cropping disease, and is applicable to modern vegetable production modes when being compared with crop rotation.
Description
Technical field
The present invention relates to a kind of disease prevention microbial inoculum and preparation method thereof, particularly relate to a kind of plant microbial inoculum of preventing and treating capsicum continuous cropping wilt disease and preparation method thereof.
Background technology
Capsicum is a kind of important solanaceous vegetables, is of high nutritive value, and wherein ascorbic content occupies first in vegetables.And the features such as the capsaicine extracting from capsicum, capsicum red pigment and capsicum seed oil are natural because having, nontoxic, have no side effect are with a wide range of applications in food, medical treatment, cosmetic and military industry.Therefore, capsicum has now become a kind of global the third-largest vegetable crop that is only second to beans, tomato, at home, is the second largest vegetable crop that is only second to Chinese cabbage.The cultivated area of China capsicum is increased to 1,300,000 hectares in 2003, accounts for world capsicum and produces 35% of area (3,700,000 hectares); China's capsicum gross yield is produced more than 2,800 ten thousand tons per year in 2005, occupies first place in the world, and accounts for half that world capsicum always produces (6,100 ten thousand tons).In global range, Asia is to produce in the world, consume the region that capsicum is maximum, is secondly Africa, and European capsicum industry proportion is minimum, but started in recent years not block, the ground such as arna A Lusi is as the major production base of capsicum, pay attention to capsicum and produce.
Along with the continuous expansion of pepper planting area and continuous cultivation throughout the year, capsicum aborning Soil-sickness Problem is day by day serious, shows as that vine growth and development is bad, disease increases the weight of, degradation under yield and quality.According to investigations, the pepper producing area continuous cropping time limit is generally 5～10 years, reaches at most 20 years, and the incidence of disease is up to 88%, dead plant rate 38%～85%.According to another report, 1 annual production of capsicum continuous cropping declines 10%～15%, and 2 years falls of continuous cropping reduce 30%～50% in 20%～30%, 3 years.Therefore, research overcomes the continuous cropping diseases that capsicum continuous cropping obstacle causes, is current capsicum production problem demanding prompt solution, also for overcoming the research of other solanaceous vegetables continuous cropping diseases, lays the foundation simultaneously.
At present, the method that overcomes capsicum wilt both at home and abroad mainly contains crop rotation, grafting and root-changing, breeding resistant variety, chemical pesticide sterilizing etc.Crop rotation is the most traditional effective method, has increased edaphon total amount, has reduced the incidence of disease.About the reason of crop rotation anti obstacle to continuous cropping, as far back as 20th century 70, the eighties, just there is report, gramineous crop and gourd, fruit and vegetable crop rotation anti obstacle to continuous cropping reason are that stalk decomposes the phenol acid substance producing, and have alleviated gourd, fruit and vegetable disease, have promoted continuous cropping plant strain growth.And specialization and the industrialized development of producing along with vegetables, crop rotation is infeasible.Grafting utilizes the advantageous feature of stock, can strengthen crop disease-resistant ability, since the eighties in last century, the countries such as Japan, Korea S begin one's study, develop graft technology, adopt disease-resistant wildness eggplant as stock, carry out grafting with cultivar, application has obtained good protection effect after producing.Enter after the nineties, China also starts to apply gradually Grafted Eggplant technology on producing, and its protection effect of the eggplant after grafting can reach more than 95%, and effect of increasing production reaches more than 30%, but graft technology is strong, is difficult for promoting.Breeding resistant variety be the important topic that vegetable disease-resistant cultivation and breeding for disease resistance field are paid close attention to, but resistance and high yield, the high-quality of kind is not also reached organic unification at present always.Chemical pesticide instant effect, successful, for capsicum wilt, before field planting, carries out soil disinfection with 1000 times of liquid of 70% fenaminosulf soluble powder, during transplanting, with 800 times of immersion root 10-15 minute of 70% fenaminosulf soluble powder.Their early stage, 500 times of liquid spraying controls of available 50% carbendazol wettable powder, but Pesticide Residue is serious.
Therefore, seek a kind of plant microbial inoculum of nontoxic, safe and effective, the control capsicum continuous cropping wilt disease that operation is simple, by significant to the control of vegetable crop continuous cropping diseases.
Summary of the invention
The object of the present invention is to provide a kind of plant microbial inoculum of preventing and treating capsicum continuous cropping wilt disease and preparation method thereof, this additive is nontoxic, has both avoided poisoning and the pollution problem to environment thereof of chemicals, effectively prevents and treats again capsicum continuous cropping wilt disease and occurs.The method is more suitable for the modern vegetables mode of production, and the effect of anti-capsicum continuous cropping wilt disease is close to crop rotation.
The object of the invention is to be achieved through the following technical solutions:
A kind of plant microbial inoculum of preventing and treating capsicum continuous cropping wilt disease, the phenolic acid that this plant microbial inoculum is maize straw, raw material comprise maize straw, water and the ethanol of pulverizing, its weight percent content is: the maize straw 40-50wt% of pulverizing, water 50-60wt%, ethanol 100-1000 milliliter.
A plant bacterial preparation process of preventing and treating capsicum continuous cropping wilt disease, described method comprises the process of being prepared as follows:
(1) take the crushing maize stalk that percentage by weight is 50wt%, the water that is 50wt% by itself and percentage by weight is mixed thoroughly, makes water saturates maize straw, and soaking the time of invading is 30 minutes;
(2) pressurization percolation extracts maize straw biochemical substances;
(3) leaching liquor after extracting, in optimum temperature: 25 ℃, adsorption liquid pH 5-8, absorption flow velocity is, under the condition of 3 ms/h-12 ms/h, through XAD-4 resin column, to adsorb;
(4) take 100-1000 milliliter ethanol as resolving agent, resolving flow velocity is 2 ms/h-3 ms/h, and phenol acid substance is resolved;
(5) ethanol of the concentrated maize straw of vacuum freeze drier is resolved be the eased additive of chilli autotoxicity of agent.
Advantage of the present invention and effect are:
1, this additive is nontoxic, safe and effective, has both avoided poisoning and the pollution problem to environment thereof of chemicals, effectively prevents and treats again capsicum continuous cropping wilt disease and occurs.Phenol acid substance in maize straw has bactericidal action to capsicum continuous cropping wilt, it directly affects the activity of pathogen, in addition, the phenol acid substance in maize straw also suppresses the activity of capsicum wilt pathogen indirectly by changing rhizospheric microorganism diversity and other rhizosphere soil biological factor.The phenol acid substance of mixed with rice bran derives from maize straw, so it is nontoxic, safely and effectively, has both avoided poisoning and the pollution problem to environment thereof of chemicals, effectively prevents and treats again capsicum continuous cropping wilt disease and occurs.
2, this additive is easy to implement when overcoming capsicum continuous cropping diseases, makes comparisons and is more suitable for the modern vegetables mode of production with wheel.The additive that utilizes the principle that counteracts each other between crop to prepare, the effect of anti-capsicum continuous cropping wilt disease is close to crop rotation, but it is easy to implement, can directly apply in capsicum continuous cropping soil, be convenient to the pepper producing area being more suitable in environmental conditions such as soil, weathers, sustainable development capsicum industry, and then make relevant corollary equipment, technology, production and marketing link be unlikely to be interrupted because of other crops of crop rotation, make capsicum industry to modernization mode developments such as specialization, industrialization.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
Embodiment 1:
The phenolic acid that the plant microbial inoculum of control capsicum continuous cropping wilt disease is maize straw.Raw material content is: 1 kilogram of the maize straw of pulverizing, 1 kilogram, 100 milliliters of ethanol.
Its preparation method:
(1), to take weight be the crushing maize stalk of 1 kilogram, the water that is 1 kilogram by itself and weight is mixed thoroughly, makes water saturates maize straw, soaking the time of invading is 30 min;
(2), pressurization percolation extracts maize straw biochemical substances;
(3) leaching liquor, after extracting, in optimum temperature: 25 ℃, adsorption liquid pH 5-8, absorption flow velocity is, under the condition of 3 ms/h-12 ms/h, through XAD-4 resin column, to adsorb.
(4), take 100ml ethanol as resolving agent, resolving flow velocity is 2 ms/h-3 ms/h, and phenol acid substance is resolved;
(5), the ethanol of the concentrated maize straw of vacuum freeze drier is resolved agent.The additive of chilli autotoxicity is eased.
Embodiment 2:
The phenolic acid that the plant microbial inoculum of control capsicum continuous cropping wilt disease is maize straw.Raw material content is: the maize straw double centner of pulverizing, double centner, ethanol 1000ml.
Its preparation method:
(1), take the crushing maize stalk that weight is double centner, the water that is double centner by itself and weight is mixed thoroughly, makes water saturates maize straw, soaking the time of invading is 30 minutes;
(2), pressurization percolation extracts maize straw biochemical substances;
(3) leaching liquor, after extracting, in optimum temperature: 25 ℃, adsorption liquid pH 5-8, absorption flow velocity is, under the condition of 3 ms/h-12 ms/h, through XAD-4 resin column, to adsorb.
(4), take 1000ml ethanol as resolving agent, resolving flow velocity is 2 ms/h-3 ms/h, and phenol acid substance is resolved;
(5), the ethanol of the concentrated maize straw of vacuum freeze drier is resolved agent.The additive of chilli autotoxicity is eased.
Claims (1)
1. a plant microbial inoculum of preventing and treating capsicum continuous cropping wilt disease, it is characterized in that, the phenolic acid that this plant microbial inoculum is maize straw, raw material comprise maize straw, water and the ethanol of pulverizing, its weight percent content is: the maize straw 40-50wt% of pulverizing, water 50-60wt%, ethanol 100-1000 milliliter;
Described plant microbial inoculum is adopted preparation with the following method:
(1) take the crushing maize stalk that percentage by weight is 40-50wt%, the water that is 50-60wt% by itself and percentage by weight is mixed thoroughly, makes water saturates maize straw, and soaking the time of invading is 30 minutes;
(2) pressurization percolation extracts maize straw biochemical substances;
(3) leaching liquor after extracting, in optimum temperature: 25 ℃, adsorption liquid pH 5-8, absorption flow velocity is, under the condition of 3 ms/h-12 ms/h, through XAD-4 resin column, to adsorb;
(4) take 100-1000 milliliter ethanol as resolving agent, resolving flow velocity is 2 ms/h-3 ms/h, and phenol acid substance is resolved;
(5) ethanol of the concentrated maize straw of vacuum freeze drier is resolved be the eased additive of chilli autotoxicity of agent.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310200593.1A CN103238639B (en) | 2013-05-27 | 2013-05-27 | Plant fungicide for preventing and treating chill continuous cropping withering disease and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104098407B (en) * | 2014-07-08 | 2016-07-06 | 淮阴工学院 | Fructus Capsici continuous cropping Auto toxicity attapulgite clay base renovation agent, its preparation method and seedling medium preparation method thereof |
| CN104387266B (en) * | 2014-12-10 | 2016-03-30 | 江苏科技大学 | A kind of method preparing the active phenolic acid of stalk |
| CN106085919A (en) * | 2016-08-03 | 2016-11-09 | 界首市沃土生物科技有限公司 | A kind of microbial bacterial agent improving Fructus Capsici continuous cropping obstacle |
| CN109438111A (en) * | 2018-11-15 | 2019-03-08 | 贵州品红农业开发有限公司 | A kind of soil conditioner and preparation method thereof on pepper planting ground |
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| CN1267457A (en) * | 1999-03-23 | 2000-09-27 | 郝锁振 | Preparation of cotton wilt preventing and treating germicide with crop straw |
| CN101723719B (en) * | 2009-11-27 | 2013-03-27 | 沈阳大学 | Additive for relieving chilli autotoxicity and preparation method thereof |
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