CN104351329B - Composite leaching vegetable fresh-keeping agent for tragus berteronianus and preparation method of fresh-keeping agent - Google Patents
Composite leaching vegetable fresh-keeping agent for tragus berteronianus and preparation method of fresh-keeping agent Download PDFInfo
- Publication number
- CN104351329B CN104351329B CN201410638904.7A CN201410638904A CN104351329B CN 104351329 B CN104351329 B CN 104351329B CN 201410638904 A CN201410638904 A CN 201410638904A CN 104351329 B CN104351329 B CN 104351329B
- Authority
- CN
- China
- Prior art keywords
- rhizome
- extract
- preparation
- fresh
- radix semiaquilegiae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention discloses a composite leaching vegetable fresh-keeping agent for tragus berteronianus and a preparation method of the fresh-keeping agent. The composite leaching vegetable fresh-keeping agent is prepared by the following steps: carrying out pretreatment on tragus berteronianus leaves, radix semiaquilegiae roots and stems and lotus persimmon leaves, then preparing extracting solutions, and concentrating and drying the mixture after mixing the extracting solutions. The composite leaching vegetable fresh-keeping agent for tragus berteronianus, disclosed by the invention, is prepared from pure natural plants in a composite leaching manner, is green, low in cost, safe, reliable, good in preservation effect, and free of harmful residues, can replace an existing chemical preservative agent in the market as a common preservative agent, is convenient to use, and adapts to vegetable preservation in all scales from small farmers to large commercial tenants.
Description
Technical field
The present invention relates to agent keeping vegetable fresh technical field, more particularly to a kind of lousewort be combined extraction agent keeping vegetable fresh and
Its preparation method.
Background technology
China is vegetables big producing country, and meanwhile, vegetables industry has also become the pillar of China's agricultural and rural economy development
Industry, cultivated area is only second to grain together with fruit, becomes Chinese second largest crops.Due to people's living standard significantly
Improve, the consciousness of health diet is thin, and operating pressure increases, amount of exercise reduces, and therefore, leads to hypertension, high fat of blood, fertilizer
The crowd of the wealth and rank type disease such as fat disease dramatically increases.In recent years, increasing people notices that rich man's disease seriously threatens us
Health, and the understanding that people live to health diet steps up.Health diet then requires us to take in animality egg less
White matter, eats vegetable and fruit more, therefore, the consumption figure cumulative year after year of vegetables.But due to physiological aging, micro- life before and after vegetables harvesting
The many reasons such as thing infringement and mechanical damage, perishable rotten, not storage tolerance.According to ASSOCIATE STATISTICS, Chinese fresh vegetables at this stage
The rotten proportion of goods damageds up to 40%～50%, and developed country's average loss rate is less than 7%.
At present, conventional fresh-keeping of vegetables method remains and adopts cryopreservation, and domestic Vegetables Production and Sale pattern is still " little
Produce, big market " pattern, it is relatively costly to which results in cryopreservation, and impracticable to smallholder.In recent years, businessman
Fresh-keeping effect and product quality, in order to extend raw vegetable postharvest storage fresh keeping time, improve in peasant household, add harmful in vegetables
Chemical substance carries out fresh-keeping, the such as fresh-keeping of vegetables security incident such as blue vitriol leek, formaldehyde Chinese cabbage, malicious ginger.Although this method can have
Effect reduces the vegetables proportion of goods damageds, but there is obvious food-safety problem, damages health, even leads to death.Therefore,
It is combined extraction fresh-keeping of vegetables in the urgent need to studying a kind of natural plants green, with low cost, safe and reliable, that fresh-keeping effect is good
Agent.
Content of the invention
The purpose of the present invention is aiming at perishable rotten after vegetables are adopted, and loss amount is big, and the one-tenth of existing preservation method
Basis, effect, safety problem, provide a kind of natural plants green, with low cost, safe and reliable, that fresh-keeping effect is good to be combined leaching
Carry agent keeping vegetable fresh, specially a kind of lousewort is combined extraction agent keeping vegetable fresh and preparation method thereof.
Heretofore described corn alcohol solution is the alcoholic solution being produced and processed out for raw material with corn.
Lotus flower persimmon is a kind of persimmon, belongs to Ebenaceae plant berry fruits, and lotus flower persimmon leaf of the present invention is above-mentioned
The leaf of plant lotus flower persimmon.
A kind of lousewort of the present invention is combined extraction agent keeping vegetable fresh and preparation method thereof technical scheme, a kind of lousewort
Louse blade of grass, radix semiaquilegiae rhizome, three kinds of raw material of lotus flower persimmon leaf are carried out pre-processing laggard by compound extraction agent keeping vegetable fresh respectively
Prepared by row extract, then obtain compound extraction agent keeping vegetable fresh by carrying out concentrate drying after the extract mixing of three.
Raw material are included by weight：45～60 parts of louse blade of grass, 30～40 parts of radix semiaquilegiae rhizome, lotus flower persimmon leaf 70～
90 parts.
A kind of described lousewort is combined the preparation method of extraction agent keeping vegetable fresh, comprises the following steps：
(1) louse blade of grass pretreatment
Weigh 45～60 parts of louse blade of grass, cleaning, draining post-drying, dried louse blade of grass is pulverized, screened 200
～300 mesh, standby；
(2) preparation of lousewort leaf extract
The louse blade of grass powder of preparation in step (1) is used corn alcohol solution to soak, then adopt ultrasonic wave extraction,
Centrifugation, standing, take supernatant that lousewort leaf extract is obtained, standby；
(3) radix semiaquilegiae rhizome pretreatment
Weigh 30～40 parts of radix semiaquilegiae rhizome mixture, cleaning, draining post-drying, by dried radix semiaquilegiae rhizome powder
Broken, screened 200～300 mesh, standby；
(4) preparation of radix semiaquilegiae rhizome extract
Above-mentioned radix semiaquilegiae rhizome powder is carried out microwave treatment using after ethanol immersion, the crude extract mistake after microwave treatment
AB-8 macroporous adsorbent resin column chromatography, collection lower column liquid, prepared radix semiaquilegiae rhizome extract, standby；
(5) preparation of lotus flower persimmon leaf extract
Weigh 70～90 parts of lotus flower persimmon leaf, be soaked in water, clean post-drying, dried lotus flower persimmon leaf is poured into pulverizer
Inside pulverized, screened 200～300 mesh, entered supercritical CO2Extract in abstraction pool, prepared lotus flower persimmon leaf extract, standby
With；
(6) preparation of compound extractant concentrate
By the radix semiaquilegiae rhizome extract being obtained in the lousewort leaf extract being obtained in step (2), step (4), step
(5) concentrated after the common mixing of lotus flower persimmon leaf extract being obtained in, prepared compound extractant concentrate, standby；
(7) it is combined the preparation of extraction agent keeping vegetable fresh
The compound extractant concentrate being obtained in step (6) is poured in vacuum drying container tank, sets and adjust dry temperature
Spend for 70～90 DEG C, vacuum is 0.08-0.095, after 24～48 h are dried, take out powder, compound extraction fresh-keeping of vegetables is obtained
Agent.
Step（2）It is specially：The louse blade of grass powder of preparation in step (1) is pressed louse blade of grass powder:Corn alcohol is molten
Liquid weight is than for 1～3:200～300 addition spirituosity mass concentrations be 60～70% corn alcohol solution mixing, soak 5～
10 h, are transferred in ultrasonic extractor, and regulation mixing speed is 60～80 rmp, and ultrasonic frequency is 40 KHZ, and temperature is
45～50 DEG C, and extract 1～1.5 h and obtain coarse body fluid, crude extract is poured in centrifuge, is centrifuged 8 with 3500～4000 rmp
～12 min, stand 2～4 h under room temperature, take supernatant that lousewort leaf extract is obtained.
Step（4）It is specially：By step（3）The radix semiaquilegiae rhizome powder obtaining is by radix semiaquilegiae rhizome powder:Ethanol weight
Than for 1～3:100～300 addition weight/mass percentage composition are the ethanol solution of 60～70 %, carry out at microwave after soaking 6～12 h
Reason, microwave power is 360～540 W, and the microwave time is 8～15 min, and the crude extract after microwave treatment crosses AB-8 macroporous absorption
Resin column chromatography, collects lower column liquid, prepared radix semiaquilegiae rhizome extract.
Step（5）It is specially：Weigh 70～90 parts of lotus flower persimmon leaf, be soaked in water 1～2 h, then cleans up, and is transferred to 50
Dry 50～60 min in～60 DEG C of drying box, dried lotus flower persimmon leaf pulverized, screened 200～300 mesh,
Pour supercritical CO again into2In abstraction pool, adjustment equipment extracting pressure is 20～30 MPa, CO2Flow velocity is 10～20 kg/h, temperature
For 40～60 DEG C, dynamic extraction time is 30～50 min, prepared lotus flower persimmon leaf extract.
A kind of described lousewort is combined the preparation method of extraction agent keeping vegetable fresh, specially：
(1) louse blade of grass pretreatment
Weigh 45～60 parts of louse blade of grass, put in pond and clean 10～15 min with Water spray, pull out and put in Dropbox
Draining 20～30 min, transfers in 50～65 DEG C of drying box and dries 40～50 min, dried louse blade of grass is fallen
Enter in pulverizer and pulverized, screened 200～300 mesh, standby；
(2) preparation of lousewort leaf extract
The louse blade of grass powder of preparation in step (1) is pressed louse blade of grass powder:Corn alcohol solution weight is than for 1～3:
200～300 addition spirituosity mass concentrations are 60～70% corn alcohol solution mixing, soak 5～10 h, are transferred to ultrasonic
In ripple extractor, power-on, regulation mixing speed is 60～80 rmp, and ultrasonic frequency is 40 KHZ, and temperature is 45～50
DEG C, and after extracting 1～1.5 h, close power supply, crude extract is poured in centrifuge, it is centrifuged 8～12 with 3500～4000 rmp
Min, stands 2～4 h under room temperature, takes supernatant that lousewort leaf extract is obtained, standby；
(3) radix semiaquilegiae rhizome pretreatment
Weigh 30～40 parts of radix semiaquilegiae rhizome mixture, be transferred in pond and clean 6～10 min with running water, pull out and put
Enter draining 10～20 min in Dropbox, transfer in 60～70 DEG C of drying box and dry 30～40 min, by dried sky
Certain herbaceous plants with big flowers rhizome is poured in pulverizer and is pulverized, and screens 200～300 mesh, standby；
(4) preparation of radix semiaquilegiae rhizome extract
Above-mentioned radix semiaquilegiae rhizome powder is poured in holding vessel, and presses radix semiaquilegiae rhizome powder:Ethanol weight is than for 1～3:
The ethanol solution of 100～300 addition weight/mass percentage composition 60～70 %, carries out microwave treatment, microwave power after soaking 6～12 h
For 360～540 W, the microwave time is 8～15 min, and the crude extract after microwave treatment crosses AB-8 macroporous adsorbent resin column chromatography,
Collection lower column liquid, prepared radix semiaquilegiae rhizome extract, standby；
(5) preparation of lotus flower persimmon leaf extract
Weigh 70～90 parts of lotus flower persimmon leaf, be transferred in pond and soak 1～2 h with running water, then clean up, be transferred to
Dry 50～60 min in 50～60 DEG C of drying box, dried lotus flower persimmon leaf is poured in pulverizer and is pulverized, screening
Cross 200～300 mesh, then pour supercritical CO into2In abstraction pool, adjustment equipment extracting pressure is 20～30 MPa, CO2Flow velocity is 10
～20 kg/h, temperature is 40～60 DEG C, and dynamic extraction time is 30～50 min, and prepared lotus flower persimmon leaf extract is standby；
(6) preparation of compound extractant concentrate
By the radix semiaquilegiae rhizome extract being obtained in the lousewort leaf extract being obtained in step (2), step (4), step
(5) the common mixing of lotus flower persimmon leaf extract being obtained in is poured in concentration tank, sets vacuum as -0.045～-0.075 Mpa,
Bath temperature is 70～80 DEG C, when being concentrated into proportion for 1.2～1.4, stops concentrating, prepared compound extractant concentrate, standby
With；
(7) it is combined the preparation of extraction agent keeping vegetable fresh
The compound extractant concentrate being obtained in step (6) is poured in vacuum drying container tank, sets and adjust dry temperature
Spend for 70～90 DEG C, vacuum is 0.08-0.095, after 24～48 h are dried, take out powder, compound extraction fresh-keeping of vegetables is obtained
Agent.
A kind of described lousewort is combined the application in fresh-keeping aspect of vegetable for the extraction agent keeping vegetable fresh, and its using method is：
By agent keeping vegetable fresh 50-85 mg, pour in 1～3 L water, mix, prepared fresh-keeping of vegetables agent solution, it is uniformly sprayed
Can carry out fresh-keeping on different vegetable surface.
Using method is specially:By agent keeping vegetable fresh 50-85 mg, pour in 1～3 L water, at room temperature with 80-120
Rmp stir 2-5 min, prepared fresh-keeping of vegetables agent solution, it is uniformly sprayed at different vegetable surface can carry out fresh-keeping.
Beneficial effects of the present invention are：The lousewort of the present invention is combined extraction agent keeping vegetable fresh and is combined leaching through pure plant
Obtain through refining and take, green, with low cost, safe and reliable, fresh-keeping effect is good, no hazard residue.Existing chemistry on market can be replaced to protect
Antistaling agent is commonly used in fresh dose of conduct, easy to use, adapts to from smallholder to the fresh-keeping of vegetables of the various scale of large-scale trade company.
Brief description：
Fig. 1 show the embodiment of the present invention 1 and reference examples 1, the comparison of reference examples 2 storage period chlorophyll content；
Fig. 2 show the embodiment of the present invention 1 and reference examples 1, the comparison of reference examples 2 storage period Vc content；
Fig. 3 show the embodiment of the present invention 1 and reference examples 1, the comparison of reference examples 2 storage period quality weight-loss ratio；
Fig. 4 show the embodiment of the present invention 2 and reference examples 3, the comparison of reference examples 4 storage period rotting rate；
Fig. 5 show the embodiment of the present invention 2 and reference examples 3, the comparison of reference examples 4 storage period weight-loss ratio.
Specific embodiment：
For a better understanding of the present invention, describe technical scheme in detail with instantiation below, but this
Invention is not limited thereto.
Embodiment 1
A kind of lousewort is combined the preparation method of extraction agent keeping vegetable fresh, comprises the following steps：
(1) louse blade of grass pretreatment
Weigh 50 parts of louse blade of grass, put in pond and clean 13 min with spray thrower, pull out and put into draining 26 in Dropbox
Min, transfers in 60 DEG C of drying box and dries 48 min, dried louse blade of grass is poured in pulverizer and is pulverized,
Screened 200 mesh, standby；
(2) preparation of lousewort leaf extract
The louse blade of grass powder of preparation in step (1) is poured in holding vessel, and presses powder:Corn alcohol solution is 1:200
Add 70% corn alcohol solution mixing, soak 6 h, be transferred in ultrasonic extractor, power-on, adjusting mixing speed is
65 rmp, ultrasonic frequency is 40 KHZ, and temperature is 45 DEG C, and after extracting 1 h, closes power supply, crude extract is poured into centrifuge
In, it is centrifuged 10 min with 3500 rmp, stand 2 h under room temperature, take supernatant that lousewort leaf extract is obtained, standby；
(3) radix semiaquilegiae rhizome pretreatment
Weigh 32 parts of radix semiaquilegiae rhizome mixture, be transferred in pond and clean 7min with running water, pull out and put in Dropbox
Draining 10 min, transfers in 60 DEG C of drying box and dries 40 min, dried radix semiaquilegiae rhizome is poured in pulverizer
Pulverized, screened 200 mesh, standby；
(4) preparation of radix semiaquilegiae rhizome extract
Above-mentioned radix semiaquilegiae rhizome powder is poured in holding vessel, and presses powder:Ethanol is 1.5:150 addition 60 % ethanol, leaching
Carry out microwave treatment, microwave power is 360 W, the microwave time is 10 min, the crude extract after microwave treatment crosses AB-8 after steeping 8 h
Macroporous adsorbent resin column chromatography, collection lower column liquid, prepared radix semiaquilegiae rhizome extract, standby；
(5) preparation of lotus flower persimmon leaf extract
Weigh 75 parts of lotus flower persimmon leaf, be transferred in pond and soak 1 h with running water, then clean up, be transferred to 50 DEG C
Dry 60 min in drying box, dried lotus flower persimmon leaf is poured in pulverizer and is pulverized, screened 200 mesh, then pour into
Supercritical CO2In abstraction pool, adjustment equipment extracting pressure is 20 MPa, CO2Flow velocity is 10 kg/h, and temperature is 40 DEG C, dynamic extraction
The time is taken to be 35 min, prepared lotus flower persimmon leaf extract, standby；
(6) preparation of compound extractant concentrate
By the radix semiaquilegiae rhizome extract being obtained in the lousewort leaf extract being obtained in step (2), step (4), step
(5) the common mixing of lotus flower persimmon leaf extract being obtained in is poured in concentration tank, sets vacuum as -0.05 Mpa, bath temperature
For 70 DEG C, when being concentrated into proportion for 1.2, stop concentrating, prepared compound extractant concentrate, standby；
(7) it is combined the preparation of extraction agent keeping vegetable fresh
The compound extractant concentrate being obtained in step (6) is poured in vacuum drying container tank, sets and adjust dry temperature
Spend for 75 DEG C, vacuum is 0.08 Mpa, after 36 h are dried, take out powder, compound extraction agent keeping vegetable fresh is obtained.
The using method of agent keeping vegetable fresh：
, weigh composite powder 50 mg being obtained in step (7), pour in 1 L clear water, in room taking Fresh-cut apple as a example
Temperature under with 80 rmp stir 2 min, prepared fresh-keeping of vegetables agent solution, be uniformly sprayed at broccoli surface, temperature be 4 DEG C, wet
Spend for preservation and freshness under 85% environment.
Reference examples 1
Difference from Example 1 is, this reference examples adopts in the patent application of Publication No. CN 102726511 B
Methods described preparation fruit and vegetable fresh-keeping agent according to optimum proportioning, film is carried out to broccoli, and temperature be 4 DEG C, humidity be 85%
Preservation and freshness under environment；
Reference examples 2
Difference from Example 1 is, this reference examples adopts in the patent application of Publication No. CN 103548994 A
The fruit and vegetable fresh-keeping agent of methods described preparation carries out film or sprinkling according to optimum proportioning to broccoli, and is 4 DEG C, humidity in temperature
For preservation and freshness under 85% environment.
The embodiment of the present invention 1 and reference examples 1, reference examples 2 storage period chlorophyll content relatively ask for an interview Figure of description
Shown in Fig. 1, in storage period, the chlorophyll content of each group all reduces with the prolongation of storage time, and wherein embodiment 1 middle period is green
Cellulose content is consistently higher than control group.Control group 1 is close with control group 2 downward trend.Fig. 1 shows that the broccoli in embodiment 1 exists
Storage Determination of Chlorophyll content reduces speed slowly, effectively maintains the color and luster of broccoli, and control group effect is substantially poor
In embodiment 1.
The embodiment of the present invention 1 and reference examples 1, reference examples 2 storage period Vc content relatively ask for an interview Figure of description Fig. 2 institute
Show, in storage period, the Vc content of each group all reduces with the prolongation of storage time, and wherein the Vc content in embodiment 1 is obvious
Higher than control group.Control group 1 is close with control group 2 fall off rate and trend, and two groups of Vc contents are significantly lower than embodiment 1.Fig. 2 table
Broccoli in bright embodiment 1 Vc content in storage reduces speed slowly, effectively slow down broccoli and rots.
The embodiment of the present invention 1 and reference examples 1, reference examples 2 storage period quality weight-loss ratio relatively ask for an interview Figure of description
Shown in Fig. 3, in storage period, the quality weight-loss ratio of each group all increases with the prolongation of storage time, wherein the matter in embodiment 1
Amount weight-loss ratio is significantly lower than control group.Control group 1 is close with control group 2 mass weight-loss ratio climbing speed and trend, two groups of quality
Weight-loss ratio is apparently higher than embodiment 1.Fig. 3 shows broccoli in embodiment 1 quality weight-loss ratio rate of rise in storage
Slowly, its mass change is minimum, effectively maintains broccoli moisture and other nutrient content.
Embodiment 2
A kind of lousewort is combined the preparation method of extraction agent keeping vegetable fresh, comprises the following steps：
(1) louse blade of grass pretreatment
Weigh 56 parts of louse blade of grass, put in pond and clean 13.5 min with spray thrower, pull out and put into draining 30 in Dropbox
Min, transfers in 60 DEG C of drying box and dries 48 min, dried louse blade of grass is poured in pulverizer and is pulverized,
Screened 300 mesh, standby；
(2) preparation of lousewort leaf extract
The louse blade of grass powder of preparation in step (1) is poured in holding vessel, and presses powder:Corn alcohol solution is 2.5:
280 add 65% corn alcohol solution mixing, soak 8 h, are transferred in ultrasonic extractor, power-on, adjust mixing speed
For 80 rmp, ultrasonic frequency is 40 KHZ, and temperature is 50 DEG C, and after extracting 1.5 h, closes power supply, by crude extract pour into from
In scheming, it is centrifuged 10 min with 3800 rmp, stands 3.5 h under room temperature, take supernatant that lousewort leaf extract is obtained, standby；
(3) radix semiaquilegiae rhizome pretreatment
Weigh 40 parts of radix semiaquilegiae rhizome mixture, be transferred in pond and clean 10 min with running water, pull out and put into Dropbox
Middle draining 17 min, transfers in 70 DEG C of drying box and dries 36 min, dried radix semiaquilegiae rhizome is poured into pulverizer
Inside pulverized, screened 300 mesh, standby；
(4) preparation of radix semiaquilegiae rhizome extract
Above-mentioned radix semiaquilegiae rhizome powder is poured in holding vessel, and presses powder:Ethanol is 1.5:180 addition 70% ethanol, soak
Carry out microwave treatment, microwave power is 540 W, the microwave time is 13 min, and it is big that the crude extract after microwave treatment crosses AB-8 after 8 h
Macroporous adsorbent resin column chromatography, collection lower column liquid, prepared radix semiaquilegiae rhizome extract, standby；
(5) preparation of lotus flower persimmon leaf extract
Weigh 80 parts of lotus flower persimmon leaf, be transferred in pond and soak 1.5 h with running water, then clean up, be transferred to 60 DEG C
Drying box in dry 55 min, dried lotus flower persimmon leaf is poured in pulverizer and is pulverized, screened 200 mesh, then fall
Enter supercritical CO2In abstraction pool, adjustment equipment extracting pressure is 25 MPa, CO2Flow velocity is 16 kg/h, and temperature is 60 DEG C, dynamically
Extraction time is 40 min, and prepared lotus flower persimmon leaf extract is standby；
(6) preparation of compound extractant concentrate
By the radix semiaquilegiae rhizome extract being obtained in the lousewort leaf extract being obtained in step (2), step (4), step
(5) the common mixing of lotus flower persimmon leaf extract being obtained in is poured in concentration tank, sets vacuum as -0.065 Mpa, bath temperature
For 80 DEG C, when being concentrated into proportion for 1.4, stop concentrating, prepared compound extractant concentrate, standby；
(7) it is combined the preparation of extraction agent keeping vegetable fresh
The compound extractant concentrate being obtained in step (6) is poured in vacuum drying container tank, sets and adjust dry temperature
Spend for 75 DEG C, vacuum is 0.095, after 42 h are dried, take out powder, compound extraction agent keeping vegetable fresh is obtained.
The using method of agent keeping vegetable fresh：
Taking " Lujiao 3 " capsicum as a example, weigh composite powder 65 mg being obtained in step (7), pour in 1.6 L clear water,
Stir 2.5 min, prepared fresh-keeping of vegetables agent solution with 100 rmp at room temperature, be uniformly sprayed at capsicum surface, be 4 in temperature
DEG C, humidity be 90% environment under preservation and freshness.
Reference examples 3
Difference from Example 2 is, this reference examples adopts in the patent application of Publication No. CN 102726511 B
Methods described preparation fruit and vegetable fresh-keeping agent according to optimum proportioning, film is carried out to " Lujiao 3 ", and temperature be 4 DEG C, humidity be
Preservation and freshness under 90% environment；
Reference examples 4
Difference from Example 2 is, this reference examples adopts in the patent application of Publication No. CN 103548994 A
Methods described preparation fruit and vegetable fresh-keeping agent according to optimum proportioning, film or sprinkling are carried out to " Lujiao 3 ", and temperature be 4 DEG C,
Humidity is preservation and freshness under 90% environment.
The embodiment of the present invention 2 and reference examples 3, reference examples 4 storage period rotting rate relatively ask for an interview Figure of description Fig. 4 institute
Show, in storage period, the rotting rate of each group is all incremented by with the prolongation of storage time, the wherein capsicum rotting rate in embodiment 2
Significantly lower than control group, almost there is not putrefactive phenomenon.Control group 3 and control group 4 are in obvious ascendant trend and close, with
The prolongation putrefactive phenomenon of time is increasingly severe.Fig. 4 shows that capsicum in embodiment 2 rotting rate in storage is extremely low, bright
Show and be better than control group.
The embodiment of the present invention 2 and reference examples 3, reference examples 4 storage period weight-loss ratio relatively ask for an interview Figure of description Fig. 5 institute
Show, in storage period, the weight-loss ratio of each group is all gradually increased with the prolongation of storage time, wherein the weight-loss ratio in embodiment 2
Significantly lower than control group.Prolongation in time raises rapidly control group 3 with control group 4 weight-loss ratio, thus leading to fresh keeping time big
Big reduction, capsicum withers.Fig. 5 shows that the capsicum in embodiment 2 maintains higher moisture in storage, and various nutrition are damaged
Lose relatively low, play superior fresh-keeping effect.
Claims (7)
1. a kind of lousewort is combined extraction agent keeping vegetable fresh it is characterised in that respectively by louse blade of grass, radix semiaquilegiae rhizome, lotus flower
Three kinds of raw material of persimmon leaf carry out extract preparation after being pre-processed, and then carry out concentrate drying by after the extract mixing of three
Obtain compound extraction agent keeping vegetable fresh；Raw material are included by weight：45～60 parts of louse blade of grass, radix semiaquilegiae rhizome 30～40
Part, 70～90 parts of lotus flower persimmon leaf；
A kind of described lousewort is combined the preparation method of extraction agent keeping vegetable fresh, comprises the following steps：
(1) louse blade of grass pretreatment
Weigh 45～60 parts of louse blade of grass, cleaning, draining post-drying, dried louse blade of grass is pulverized, screened 200～
300 mesh, standby；
(2) preparation of lousewort leaf extract
The louse blade of grass powder of preparation in step (1) is used corn alcohol solution to soak, then adopt ultrasonic wave extraction, from
The heart, standing, take supernatant that lousewort leaf extract is obtained, standby；
(3) radix semiaquilegiae rhizome pretreatment
Weigh 30～40 parts of radix semiaquilegiae rhizome mixture, cleaning, draining post-drying, dried radix semiaquilegiae rhizome is pulverized, sieve
Selected 200～300 mesh, standby；
(4) preparation of radix semiaquilegiae rhizome extract
Above-mentioned radix semiaquilegiae rhizome powder is carried out microwave treatment using after ethanol immersion, it is big that the crude extract after microwave treatment crosses AB-8
Macroporous adsorbent resin column chromatography, collection lower column liquid, prepared radix semiaquilegiae rhizome extract, standby；
(5) preparation of lotus flower persimmon leaf extract
Weigh 70～90 parts of lotus flower persimmon leaf, be soaked in water, clean post-drying, dried lotus flower persimmon leaf is poured in pulverizer
Row is pulverized, and screens 200～300 mesh, enters supercritical CO2Extract in abstraction pool, prepared lotus flower persimmon leaf extract, standby；
(6) preparation of compound extractant concentrate
By in the radix semiaquilegiae rhizome extract being obtained in the lousewort leaf extract being obtained in step (2), step (4), step (5)
Concentrated after the common mixing of prepared lotus flower persimmon leaf extract, prepared compound extractant concentrate, standby；
(7) it is combined the preparation of extraction agent keeping vegetable fresh
By in step (6) be obtained compound extractant concentrate pour in vacuum drying container tank, sets adjust baking temperature as
70～90 DEG C, vacuum is 0.08-0.095, takes out powder after 24～48 h are dried, and compound extraction agent keeping vegetable fresh is obtained.
2. a kind of lousewort according to claim 1 is combined extraction agent keeping vegetable fresh it is characterised in that step（2）Specifically
For：The louse blade of grass powder of preparation in step (1) is pressed louse blade of grass powder:Corn alcohol solution weight is than for 1～3:200～
300 add spirituosity mass concentration to be 60～70% corn alcohol solution mixing, soak 5～10 h, are transferred to ultrasonic extractor
In, regulation mixing speed is 60～80 rmp, and ultrasonic frequency is 40 KHZ, and temperature is 45～50 DEG C, and extracts 1～1.5 h
Obtain coarse body fluid, crude extract is poured in centrifuge, be centrifuged 8～12 min with 3500～4000 rmp, under room temperature, stand 2～4
H, takes supernatant that lousewort leaf extract is obtained.
3. a kind of lousewort according to claim 1 is combined extraction agent keeping vegetable fresh it is characterised in that step（4）Specifically
For：By step（3）The radix semiaquilegiae rhizome powder obtaining is by radix semiaquilegiae rhizome powder:Ethanol weight is than for 1～3:100～300 add
Entering the ethanol solution that weight/mass percentage composition is 60～70 %, after soaking 6～12 h, carry out microwave treatment, microwave power is 360～
540 W, the microwave time is 8～15 min, and the crude extract after microwave treatment crosses AB-8 macroporous adsorbent resin column chromatography, collects lower prop
Liquid, prepared radix semiaquilegiae rhizome extract.
4. a kind of lousewort according to claim 1 is combined extraction agent keeping vegetable fresh it is characterised in that step（5）Specifically
For：Weigh 70～90 parts of lotus flower persimmon leaf, be soaked in water 1～2 h, then cleans up, be transferred in 50～60 DEG C of drying box and dry
Dry 50～60 min, dried lotus flower persimmon leaf are pulverized, screened 200～300 mesh, then pour supercritical CO into2Extraction
Chi Zhong, adjustment equipment extracting pressure is 20～30 MPa, CO2Flow velocity is 10～20 kg/h, and temperature is 40～60 DEG C, dynamic extraction
The time is taken to be 30～50 min, prepared lotus flower persimmon leaf extract.
5. a kind of lousewort according to claim 1 is combined extraction agent keeping vegetable fresh it is characterised in that its preparation method has
Body is：
(1) louse blade of grass pretreatment
Weigh 45～60 parts of louse blade of grass, put in pond and clean 10～15 min with Water spray, pull out and put into draining in Dropbox
20～30 min, transfer in 50～65 DEG C of drying box and dry 40～50 min, dried louse blade of grass is poured into powder
Pulverized in broken machine, screened 200～300 mesh, standby；
(2) preparation of lousewort leaf extract
The louse blade of grass powder of preparation in step (1) is pressed louse blade of grass powder:Corn alcohol solution weight is than for 1～3:200
～300 addition spirituosity mass concentrations are 60～70% corn alcohol solution mixing, soak 5～10 h, are transferred to ultrasonic wave and carry
Take in machine, power-on, regulation mixing speed is 60～80 rmp, and ultrasonic frequency is 40 KHZ, and temperature is 45～50 DEG C,
And after extracting 1～1.5 h, close power supply, crude extract is poured in centrifuge, it is centrifuged 8～12 min with 3500～4000 rmp,
Stand 2～4 h under room temperature, take supernatant that lousewort leaf extract is obtained, standby；
(3) radix semiaquilegiae rhizome pretreatment
Weigh 30～40 parts of radix semiaquilegiae rhizome mixture, be transferred in pond and clean 6～10 min with running water, pull out and put into net
Draining 10～20 min in disk, transfers in 60～70 DEG C of drying box and dries 30～40 min, by dried radix semiaquilegiae
Rhizome is poured in pulverizer and is pulverized, and screens 200～300 mesh, standby；
(4) preparation of radix semiaquilegiae rhizome extract
Above-mentioned radix semiaquilegiae rhizome powder is poured in holding vessel, and presses radix semiaquilegiae rhizome powder:Ethanol weight is than for 1～3:100
～300 addition weight/mass percentage composition are the ethanol solution of 60～70 %, carry out microwave treatment, microwave power after soaking 6～12 h
For 360～540 W, the microwave time is 8～15 min, and the crude extract after microwave treatment crosses AB-8 macroporous adsorbent resin column chromatography,
Collection lower column liquid, prepared radix semiaquilegiae rhizome extract, standby；
(5) preparation of lotus flower persimmon leaf extract
Weigh 70～90 parts of lotus flower persimmon leaf, be transferred in pond and soak 1～2 h with running water, then clean up, it is transferred to 50～
Dry 50～60 min in 60 DEG C of drying box, dried lotus flower persimmon leaf is poured in pulverizer and is pulverized, screened
200～300 mesh, then pour supercritical CO into2In abstraction pool, adjustment equipment extracting pressure is 20～30 MPa, CO2Flow velocity be 10～
20 kg/h, temperature is 40～60 DEG C, and dynamic extraction time is 30～50 min, and prepared lotus flower persimmon leaf extract is standby；
(6) preparation of compound extractant concentrate
By in the radix semiaquilegiae rhizome extract being obtained in the lousewort leaf extract being obtained in step (2), step (4), step (5)
The common mixing of prepared lotus flower persimmon leaf extract is poured in concentration tank, sets vacuum as -0.045～-0.075 Mpa, water-bath
Temperature is 70～80 DEG C, when being concentrated into proportion for 1.2～1.4, stops concentrating, prepared compound extractant concentrate, standby；
(7) it is combined the preparation of extraction agent keeping vegetable fresh
By in step (6) be obtained compound extractant concentrate pour in vacuum drying container tank, sets adjust baking temperature as
70～90 DEG C, vacuum is 0.08-0.095, takes out powder after 24～48 h are dried, and compound extraction agent keeping vegetable fresh is obtained.
6. a kind of lousewort is combined the application in fresh-keeping aspect of vegetable for the extraction agent keeping vegetable fresh as claimed in claim 1, and it is special
Levy and be, its using method is：By agent keeping vegetable fresh 50-85 mg, pour in 1～3 L water, mix, prepared fresh-keeping of vegetables
Agent solution, it is uniformly sprayed at different vegetable surface can carry out fresh-keeping.
7. application according to claim 6 is it is characterised in that using method is specially:By agent keeping vegetable fresh 50-85
Mg, pours in 1～3 L water, at room temperature with 80-120 rmp stirring 2-5 min, prepared fresh-keeping of vegetables agent solution, and it is uniform
Be sprayed at different vegetable surface can carry out fresh-keeping.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410638904.7A CN104351329B (en) | 2014-11-13 | 2014-11-13 | Composite leaching vegetable fresh-keeping agent for tragus berteronianus and preparation method of fresh-keeping agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410638904.7A CN104351329B (en) | 2014-11-13 | 2014-11-13 | Composite leaching vegetable fresh-keeping agent for tragus berteronianus and preparation method of fresh-keeping agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104351329A CN104351329A (en) | 2015-02-18 |
| CN104351329B true CN104351329B (en) | 2017-02-08 |
Family
ID=52518722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410638904.7A Expired - Fee Related CN104351329B (en) | 2014-11-13 | 2014-11-13 | Composite leaching vegetable fresh-keeping agent for tragus berteronianus and preparation method of fresh-keeping agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104351329B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106212663A (en) * | 2016-08-08 | 2016-12-14 | 山东商业职业技术学院 | A kind of natural plant antistaling agent and preparation method thereof |
| CN107343531A (en) * | 2017-07-05 | 2017-11-14 | 天津大学 | Broccoli ethanol solution handles fresh-keeping method |
| CN107197941A (en) * | 2017-07-25 | 2017-09-26 | 合肥仙之峰农业科技有限公司 | A kind of agent keeping vegetable fresh and preparation method thereof |
| CN107307075A (en) * | 2017-07-31 | 2017-11-03 | 贵州盛达生植物发展有限公司 | A kind of dendrobium candidum antistaling agent and preparation method thereof |
| CN109090229A (en) * | 2018-08-28 | 2018-12-28 | 靖西海越农业有限公司 | A kind of citrus composite preservative and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030045719A (en) * | 2003-04-29 | 2003-06-11 | 주식회사 케이티앤지 | Natural preparation preserving freshness of green ginseng and a method for improving the preservation of green ginseng by using the preparation |
| CN1672536A (en) * | 2004-03-24 | 2005-09-28 | 上海市农业科学院作物育种栽培研究所 | Extraction process of plant mildew-proof preservative |
| CN101422191A (en) * | 2008-08-18 | 2009-05-06 | 张岱银 | Plant food preservative and production method thereof |
| CN102726511A (en) * | 2012-06-22 | 2012-10-17 | 杨凌农科大无公害农药研究服务中心 | Botanical fresh keeping agent for fruits and vegetables, and preparation method thereof |
| CN103520030A (en) * | 2013-09-29 | 2014-01-22 | 上海莱博生物科技有限公司 | Preparation method of Chinese herbal compound with natural anti-corrosion effect |
| CN103548994A (en) * | 2013-11-08 | 2014-02-05 | 成都新朝阳作物科学有限公司 | Fruit and vegetable fresh-keeping agent as well as application thereof |
-
2014
- 2014-11-13 CN CN201410638904.7A patent/CN104351329B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030045719A (en) * | 2003-04-29 | 2003-06-11 | 주식회사 케이티앤지 | Natural preparation preserving freshness of green ginseng and a method for improving the preservation of green ginseng by using the preparation |
| CN1672536A (en) * | 2004-03-24 | 2005-09-28 | 上海市农业科学院作物育种栽培研究所 | Extraction process of plant mildew-proof preservative |
| CN101422191A (en) * | 2008-08-18 | 2009-05-06 | 张岱银 | Plant food preservative and production method thereof |
| CN102726511A (en) * | 2012-06-22 | 2012-10-17 | 杨凌农科大无公害农药研究服务中心 | Botanical fresh keeping agent for fruits and vegetables, and preparation method thereof |
| CN103520030A (en) * | 2013-09-29 | 2014-01-22 | 上海莱博生物科技有限公司 | Preparation method of Chinese herbal compound with natural anti-corrosion effect |
| CN103548994A (en) * | 2013-11-08 | 2014-02-05 | 成都新朝阳作物科学有限公司 | Fruit and vegetable fresh-keeping agent as well as application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 柿子叶提取物对思茅松毛虫肠道细菌抑制作用的研究;朱丽丽 等;《食品工业科技》;20131231;第34卷(第7期);第123页的摘要及第124页的1.1 * |
| 紫背天葵提取物的抑菌活性研究;罗开梅 等;《漳州师范学院学报（自然科学版）》;20111231(第4期);第83页的摘要、第84页的2.2.1及第86页的"4结论" * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104351329A (en) | 2015-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104351329B (en) | Composite leaching vegetable fresh-keeping agent for tragus berteronianus and preparation method of fresh-keeping agent | |
| CN109757683B (en) | Preparation method of whole-fruit citrus peel | |
| CN109170841A (en) | A kind of preparation method of edible fungi instant crisp chip | |
| CN105010681B (en) | A kind of black tartary buckwheat embryo tea and preparation method thereof | |
| CN107474944A (en) | A kind of processing method for promoting camellia seed oil generation | |
| CN106172730A (en) | A kind of Corm Eleocharitis peel extract bacteriostasis, preservation agent and preparation and application | |
| Đurić et al. | Use of different types of extracts as biostimulators in organic agriculture | |
| KR101375427B1 (en) | Enzymatic method for preparing gold salt | |
| CN110477173A (en) | A kind of processing method of Coffee pulp tea | |
| CN105906421A (en) | A blooming period prolonging nutrient solution for arranged flowers | |
| CN103238639B (en) | Plant fungicide for preventing and treating chill continuous cropping withering disease and preparation method thereof | |
| CN107836683A (en) | A kind of sweet potato leaves jelly | |
| CN109134082A (en) | A kind of application for pressing leaf anthocyanidin in lily nutrient solution | |
| CN104256890A (en) | Method for preventing excessive browning reaction due to improper tobacco leaf curing | |
| CN103169049A (en) | Pickled cabbage with brassica napobrassica as main raw material and making method thereof | |
| KR20170025300A (en) | Manufacturing method of soybean paste using ginseng extract and soybean paste thereof | |
| CN105105103A (en) | Helvella leucopus pers fruiting body crude polysaccharides and method for producing highly processed products thereof | |
| CN101708039B (en) | Method for preparing date powder | |
| CN110423659A (en) | It is a kind of using Chinese yew as the preparation method of the health liquor of major ingredient and health liquor | |
| CN110229730A (en) | A kind of nutrition blueberry wine and preparation method thereof | |
| CN110317109A (en) | A kind of preparation method of cherry tree special fertilizer that preventing and treating subterranean pest-insect | |
| CN108464465B (en) | Preparation method of fermented grape whole fruit powder | |
| CN111642255B (en) | Low-temperature drying method for oil tea fruits | |
| Naqash et al. | Peach Wastes and By-Products: Chemistry, Processing, and Utilization | |
| CN107446743A (en) | A kind of liquor made from sorghum and its brewing method of Chinese toon flavor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170208 Termination date: 20201113 |