CN100463604C - Method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice - Google Patents
Method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice Download PDFInfo
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- CN100463604C CN100463604C CNB2006100486119A CN200610048611A CN100463604C CN 100463604 C CN100463604 C CN 100463604C CN B2006100486119 A CNB2006100486119 A CN B2006100486119A CN 200610048611 A CN200610048611 A CN 200610048611A CN 100463604 C CN100463604 C CN 100463604C
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Abstract
The invention relates to a method for using nothosmyrnol eupat juice to restrain the plant pathogenic bacteria. The invention uses water to break the fresh nothosmyrnol eupat and takes off the juice to be sprayed on the plant, or else, the invention immerges the nothosmyrnol eupat with the water at the volume ratio as 5:1, takes off the upper clean solution to be diluted by water while the volume ratio is 1:1, to be sprayed on the plant. Said pathogenic bacteria can be notoginseng ring spot, root rot, or the like. The invention has strong restrain and disinfection functions.
Description
Affiliated technical field
The chemical composition that the invention belongs to plant is the method for the prevention and elimination of disease and pests of feature.
Background technology
Eupatorium adenophorum (Eupatorium adenophorum) is the perennial poisonous weeds of a kind of composite family Eupatorium, and plant is high by 1
-2m, the life-span can reach 12
-15 years, in Yunnan, Sichuan, Guizhou, Guangxi and Tibet has widely and distribute.As invasive species, Eupatorium adenophorum breeds rapidly and spreads, and sets up single dominant population, and agricultural, forestry, animal husbandry, bio-diversity and natural ecological environment have been constituted grave danger.
Yunnan Province is subjected to Eupatorium adenophorum harm serious area, and in recent years, the scientific worker has also carried out the research of comprehensive utilization Eupatorium adenophorum when actively seeking series control Eupatorium adenophorum harm measure.Bibliographical information, eupatorium adenophorum extracting solution is to rice weevil, corn weevil, adzuki bean weevil, four kinds of grain storage pests such as broad bean weevil have strong smoked kill activity (Li Yunshou, Zou Huaying, Wang Luxiang, Deng. purple stem boneset extract is to the insecticidal activity of four kinds of grain storage pests. insect knowledge, 2001,38 (3): 214～216.), studies show that purple stem boneset extract has stronger toxic action (Wang Yiding, Gao Ping to cotten aphid (A phis gossypii), Zhang Qihong, Deng the go out separation of aphid active substance of. Eupatorium adenophorum, purifying and to the influence of cotten aphid acetylcholinesterase. high-tech communication, 2002, (9): 21
-23.), measurement result shows that purple stem boneset extract has certain antifeedant activity (Zhou Tianxiong to the pieris rapae larva, Yang Meilin, Gu Fang, etc. purple stem boneset extract is measured the antifeedant activity of pieris rapae larva. Yunnan Prov Agriculture University's journal, 2003,18 (3): 259～263.).But utilize the bigger phytopathogen of harm in the Eupatorium adenophorum control agricultural production, study less.
Plant by virus infections after, form occurs unusual: mosaic disease or yellow illness appear in leaf chlorosis or variable color; Plant is short and small, clump branch or deformity; Cell and lesion tissue or necrosis, plant is withered or rotten.The present invention relates to 15 plant species germs, comprising:
Its pathogen of pseudo-ginseng circle pinta (round spot of Panax notoginseng) is maple bacterium thorn spore Mycocentrospora acerina (Hartig) eighton of imperfect fungus each position of harmful pseudo-ginseng Panaxnoto inseng (Burk.) F.H.Chen of should being critically ill, blade, petiole and the branch handle browning look of being injured, rot, come off, folding hangs down, and is the main disease of pseudo-ginseng acrial part.
This bacterium of notoginseng root rot (Fusarium scirpi lamb.) is false monospore bacillus (Pseudomonas sp.), belongs to Deuteromycotina, Hyphomycetes, knurl seat spore order, Tuberculariaceae, fusarium fungus.Germ is invaded from root, causes the pseudo-ginseng root-rot, blade chlorisis flavescence or blue or green withered, and germ is propagated with invalid body, soil and seedling.
Pseudo-ginseng sickle-like bacteria (Fusarium solani) cause of disease is Fusari μ m solani (Mart.) Sacc.f.sp.radicicola (Wr.) Snyd.﹠amp; Hans claims eggplant sickle spore root disease specialized form, belongs to the Deuteromycotina fungi.Make the flavescence of leaf look, growing way is poor.Root is caught an illness and is yellowish-brown or rots, and commonly putresces with the Yang Changchu of rhizome portion, and the later stage old complaint becomes pitchy or canescence, the sposh pulpous state.Annual high-temperature high humidity season morbidity in 5-August is heavy.
Pseudo-ginseng rhizoctonia (Verticillium cinnabarium) cause of disease is Rhizoctonia solani Kuhn, belongs to Deuteromycotina, Hyphomycetes, agonomycetales, Rhizoctonia fungi.Mainly the cause harm basal part of stem of pseudo-ginseng seedling of this disease is being fallen ill apart from native table 4～6cm place usually.Scab yellowish-brown recess on children's stem, strip.When serious, scab is deep into young stem interior tissue, and sick portion fractures, and the seedling lodging is dead.
Dalmatian chrysanthemum sclerotium germ (Scletrotinia sclerotiorum) pathogen is Sclerotinia (Scletrotinia), sclerotinite (Scletrotinia sclerotiorum (Lib.) De Bary) the sclerotium germ is the stronger fungi of saprophytic property, sclerotium environment to external world has stronger resistivity, can cause the stem rot (stemrot) or the white mould (whitemold) of multiple broad leaf crops such as rape, soybean, sunflower, be to influence the main disease of rape production, the middle and lower reach of Yangtze River and the production loss of southwestern producing region are reached more than 50%.
Deinsectization pine root fungus (Pestalotiopsis photiniae) cause of disease is Fusarium solani (Mart.) Sacc, be a kind of sickle spore root rot, belong to the Deuteromycotina fungi.Be the main disease of solanaceous vegetables root, this disease becomes the strain phase all to take place in the seedbed with the land for growing field crops, causes the withered of a large amount of dead seedlings and plant, and serious with winter and morbidity in early spring, the general incidence of disease reaches more than 50% when serious below 10%.
Grape anthracnose (Colletotrichum gloeosporioides) cause of disease is Glomerella singulata, i.e. colletotrichum gloeosporioides Penz (Colletotrichum gloeosporioides (Penz.) Sacc.).This bacterium optimum temperature is 28～30 ℃, and the pH value is 6.Under the condition of alternation of light and darkness, the germ growth is the fastest.The host range of this bacterium is extensive, once causes serious harm interiorly, and is bigger to grape production influence.
Tobacco brown spot pathogen (Alternaria alternata) cause of disease is interlinkage spore (Alternaria alternata (Fries) Keissler), belongs to the imperfect fungus fungi.This disease produces a large amount of dark browns or black splotch at cigarette base, Ye Zhongmai, bennet and capsule, and scab closes and makes full leaf become fragment.If environment is suitable, germ just by the plant bottom to vertical spread, can cause heavy losses.
The asexual stage of Pyricularia oryzae (Pyricularia oryzae) Pyricularia oryzae is Pyricularia oryzaeCav., belongs to the piricularia oryzae of Deuteromycotina, and the perfect stage belongs to Ascomycotina, but few the appearance.Pyricularia oryzae (Magnaporthe grisea, asexual generation is Pyricularia grisea) rice blast that causes is the widest popular, worldwide rice disease that harm is the heaviest, this disease all has serious harm in China southern and northern rice district, the popular area of disease, the general underproduction 10%
-20%, heavy reaches 40%
-50%, even No kernels or seeds are gathered, as in a year of scarcity, and grain-production is caused serious threat.
Sweet potato black rot bacterium (Ceratocystis fimbriata) pathogen is Ceratocystis fimbriata (Ceratocystis fimbriataEll.﹠amp; Halsted), the long beak shell of Ascomycotina belongs to.Sweet potato in the seedbed, land for growing field crops and shelf time all can be injured, and causes that dead seedling and potato piece rot.The scab pilosity of potato piece of being injured is born in insect bite, damaged by rats, breach place, pitchy, and circular or irregular shape, central authorities cave in slightly, and profile is clear, and diameter 1～5cm often produces the mould layer of grey (mycelium and conidium) and black thorn (perithecium) on the scab.Yellow-white wax shape granule (ascospore) often appears in black thorn top.The sick potato of stripping and slicing, visible scab lower-hierarchy is black or blackish green, and potato meat has bitter taste.
Carnation sickle-like bacteria (Fusarinm oxysporum) carnation Fusarium oxysporum [Fusarinm oxysporum Schlecht.F.sp.Dianthi (Prill.et Del.) Snyder ﹠amp; Hansen], belong to Deuteromycotina.Germ can survive in soil with host's plant residue, invades plant root and transplants by wound.Harm is rampant under 26-28 ℃ of condition.This bacterium can only be infected China pink and carnation.Stem deliquescing after being injured, easily lodging, the thin out yellow of leaf, the brown striped appears in vascular bundle inside usually, causes that plant is withered.
Konjaku bacterium (Erwinia carotovora) pathogenetic bacteria erwinia carotovora (jones) holland and erwinia arodieae (towns.) holl, its form is very similar, belongs to Erwinia.The germ unit cell is rod-short, Zhousheng 2-8 root flagellum, and host range is wide, except that konjaku, also infects Solanaceae, Cruciferae, crop such as melon.The unsetting water soaking mode crineous of susceptible bulb scab is inwardly expanded, and makes the white tissue become greyish white even yellowish-brown, and bulb is softening, rots into thick-pasty, has a large amount of bacterium hydrorrheas to go out, and gives out vinosity.Cause crop to rot at kind of a taro seeding stage, stem tuber vegetative period and kind bud or stem tuber shelf time.
Lily sickle-like bacteria (Fusarium bulbigenum) pathogen: bulbous sickle-like bacteria (Fusarium bulbigenum CookeetMass.) belongs to Deuteromycotina.This bacterium is distributed widely in rotten bulb, stem tuber, root, stem, fruit and the soil, main harm Liliaceae, green onion section, Asparagus, Crocus sativus, Freesia, gladiolus, Hyacinthus, Narcissus plant cause scale to rot the blade flavescence, do not bloom, plant is dead in early days.
Mould (Pythium aphanidermatum) the cause of disease Pythium aphanidermatum of melon and fruit corruption (Eds.) Fitzp claims that the melon and fruit corruption is mould, belongs to the Mastigomycotina fungi.The mycelia of saprogenesis also can produce sporangium in soil, infects seedling with zoospore and causes and damping off.Infecting again of field is main by output sporangium and zoospore on the sick seedling, borrows irrigation water or rainwater to spatter on the rhizome that is attached to ground proximity and causes heavy losses.
Pomegranate germ (Punica granatumL.) cause of disease Zythia versoniana Sacc. claims pomegranate bright shell spore, belongs to the Deuteromycotina fungi.The harm of pomegranate disease is to cause bud, flower, fruit early fallout, and the fruit quality of catching an illness reduces, and loses economic worth.
Summary of the invention
The objective of the invention is to utilize Eupatorium adenophorum juice to suppress the control phytopathogen, and provide the Eupatorium adenophorum treatment fluid to suppress the phytopathogen method.
The present invention is realized by following approach: convert water fresh Eupatorium adenophorum is smashed to pieces, taking juice sprays band pathogen plant.
Broken liquid of described Eupatorium adenophorum and water, 5:1 soaked 5-12 hour by volume, got supernatant, with after supernatant and the water volume ratio 1:1 dilution, sprayed band pathogen plant again.
The described pathogen plant that sprays is pseudo-ginseng circle pinta, notoginseng root rot, Dalmatian chrysanthemum sclerotium germ, deinsectization pine root fungus grape anthracnose, tobacco brown spot pathogen, Pyricularia oryzae, sweet potato black rot bacterium pseudo-ginseng sickle-like bacteria, pseudo-ginseng rhizoctonia, carnation sickle-like bacteria, konjaku bacterium, lily sickle-like bacteria, mould, the pomegranate germ disease of melon and fruit corruption.
Utilize the Eupatorium adenophorum treatment fluid that above 15 kinds of pathogens are cooked and suppress experiment, the result shows that the Eupatorium adenophorum treatment fluid all has in various degree stronger inhibition and sterilization effect to 15 kinds of pathogens.This result comes prevention and elimination of disease and pests to establish certain basis for utilizing abundant Eupatorium adenophorum resource.
Description of drawings
Fig. 1 is that Eupatorium adenophorum suppresses liquid to 15 kinds of pathogens inhibition experimental result curve synoptic diagrams.
Specific implementation method
(one) Eupatorium adenophorum suppresses the liquid preparation
(1) get fresh Eupatorium adenophorum, water is converted on the limit, and fresh Eupatorium adenophorum is smashed on the limit to pieces, and taking juice is standby.
(2) get fresh Eupatorium adenophorum, 5 parts of broken liquid of Eupatorium adenophorum are converted 1 part of water by volume, soaked overnight, and it is standby to extract supernatant liquor.
(2) Eupatorium adenophorum suppresses liquid bacteriostasis contrast experiment
Cultivate for the examination pathogen: the PDA plate that will inoculate pathogen is put into 28 ℃ insulating box and is cultivated 2～3d, when bacterium colony covers with whole plate by the time, get the bacterium piece of identical size with φ 9mm card punch, be inoculated in respectively on the culture medium flat plate that evenly has been coated with the 1ml Eupatorium adenophorum immersion liquid that above preparation method (2) provides in advance, have the contrast of 1ml sterile water in addition, each is handled and repeats 2 times, measures the diameter of pathogen bacterium colony on the variable concentrations marsh liquid culture medium flat board.
The bacteriostasis rate computing formula:
Result such as table 1:
The Eupatorium adenophorum immersion liquid is to the inhibiting rate (%) of 15 kinds of pathogens in the table 16 days
| First day | Second day | Younger brother: big | The 4th day | The 5th day | The 6th day | ||
| 1 | Pseudo-ginseng circle pinta bacterium round spot of Panax notginseng | 0 | 7.7 | 7.1 | 6.7 | 17.6 | 40 |
| 2 | The lame germ Fusaium of pseudo-ginseng |
0 | 9.1 | 37.5 | 45.4 | 45.7 | 41.2 |
| 3 | Dalmatian chrysanthemum stalk break Scletrotinia sclerotiorum | 0 | 20 | 46.7 | 60 | 70 | 80 |
| 4 | The lame germ Verticillium of |
0 | 13.3 | 67.5 | 66.0 | 66.2 | 70.6 |
| 5 | Grape anthracnose Colletotrichumgloeosporioides | 0 | 9.1 | 37.5 | 45.4 | 45.7 | 41.2 |
| 6 | Tobacco brown spot pathogen Alternaria alternata | 0 | 60 | 74.3 | 62.2 | 50.9 | 52.9 |
| 7 | Pyricularia oryzae Pyricularia oryzae | 0 | 23.1 | 50 | 56 | 66.3 | 83.1 |
| 8 | Sweet potato black rot bacterium Ceratocystis fimbriata | 0 | 33.3 | 74.2 | 82.2 | 81.7 | 71.4 |
| 9 | Pseudo-ginseng sickle-like bacteria Fusar |
0 | 47 | 63.3 | 65.9 | 64.3 | 63 |
| 10 | Pseudo-ginseng silk nuclear flower bud Verticillim cinnabarium | 0 | 46.7 | 57.9 | 66.7 | 72.4 | 63 |
| 11 | Carnation sickle-like bacteria Fusarinm oxysporum | 0 | 31.2 | 53.3 | 50 | 50 | 52.9 |
| Konjaku bacterium Erminia carotovora | 0 | 7.7 | 7.1 | 6.7 | 17.6 | 40 | |
| 13 | Lily sickle-like bacteria Fusarium bulbigenum | 0 | 38.5 | 52.2 | 65 | 70.9 | 77.1 |
| 14 | The rotten mould Pythium aphanidermatum of melon and |
0 | 7.7 | 7.1 | 6.7 | 17.6 | 40 |
| 15 | The |
0 | 9.1 | 37.5 | 45.4 | 45.7 | 41.2 |
| 16 | Punica |
0 | 0 | 0 | 0 | 0 | 0 |
(three) Eupatorium adenophorum suppresses the liquid use
The Eupatorium adenophorum that preparation is standby suppresses liquid and sprays the plant that has pathogen, promptly gets fresh Eupatorium adenophorum juice, or with after supernatant and the water 1:1 dilution by volume, sprays the plant that has pathogen.
Claims (3)
1. the method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice is characterized in that converting water fresh Eupatorium adenophorum is smashed to pieces, and taking juice sprays the plant of being with pathogen.
2. the method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice according to claim 1 is characterized in that 5 parts of broken liquid of Eupatorium adenophorum are converted 1 part of water by volume, soaks 5
-12 hours, get supernatant, with after supernatant and the water volume ratio 1:1 dilution, spray the plant of band pathogen again.
3. the method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice according to claim 1 is characterized in that the pathogen that sprays is pseudo-ginseng circle pinta, notoginseng root rot, Dalmatian chrysanthemum sclerotium germ, deinsectization pine root fungus, grape anthracnose, tobacco brown spot pathogen, Pyricularia oryzae, sweet potato black rot bacterium, pseudo-ginseng sickle-like bacteria, pseudo-ginseng rhizoctonia, carnation sickle-like bacteria, konjaku bacterium, lily sickle-like bacteria, mould, the pomegranate germ disease of melon and fruit corruption.
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