CN101280320A - Method for preparing antibiotic substance from chaetomium globosum of plant endophytic fungi - Google Patents
Method for preparing antibiotic substance from chaetomium globosum of plant endophytic fungi Download PDFInfo
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- CN101280320A CN101280320A CNA2008100153887A CN200810015388A CN101280320A CN 101280320 A CN101280320 A CN 101280320A CN A2008100153887 A CNA2008100153887 A CN A2008100153887A CN 200810015388 A CN200810015388 A CN 200810015388A CN 101280320 A CN101280320 A CN 101280320A
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Abstract
The invention relates to a preparation method for producing antibiotic substance by endophytic fungi Chaetomium globosum ND35. The endophytic fungi Chaetomium globosum ND35 is shaking cultured to produce the antibiotic substance by mainly adopting corn meal or potato sucrose liquid medium. After fermentation liquor is filtrated by gauze, centrifugal supernatant is extracted 3 times using ethyl acetate of identical volume. After being dried by using anhydrous sodium sulfate, extract liquid is performed filtration and rotary evaporation, to obtain tawny solid antibiotic substance. The antibiotic substance can effectively control various plant fungal diseases, and simultaneously guarantee the safety of human and livestock and protect the environment. The invention has the advantages that the performance is stable, the toxicity is low, and the invention is toxicological harmless to human and livestock, and has remarkable effect in guarding against and curing plant fungal diseases. The invention lays foundations for the further development and the application of antifungal antibiotic produced by endophytic fungi Chaetomium globosum ND35.
Description
(1) invention field
The present invention relates to the preparation method that plant endogenesis epiphyte chaetomium globosum ND35 produces antibiotics.
(2) background technology
In recent years, because the simplification of Cultivar and the raising of multiple crop index, the Plant diseases that is caused by pathogen is on the rise, and all cause considerable damage to agriculture production every year.Usually, chemical pesticide is protective plant effectively, but excessively uses chemical pesticide not only to improve the resistance of pathogen, and causes environmental pollution and influence human health.The chemical pesticide that the public is concerned about has promoted a kind of relatively research of the method for preventing and treating of safety the resistance that the harmful effect and the pathogen of environment and human health developed.Biological control is a kind of eco-friendly and chemical pesticide effectively replenished or the alternate control measures.Therefore biological control has been subjected to special concern as the rising method of preventing and treating.Seeking method efficient, safe, persistent controlling plant diseases is the task of top priority.
Utilizing microorganism live body microbial inoculum controlling plant diseases is one of traditional biological and ecological methods to prevent plant disease, pests, and erosion technology, though obtained certain effect, use often owing to various Effect of Environmental cause the protection effect instability in the field.Put into practice verifiedly, from the fermentation culture of biocontrol microorganisms, extract the preparation antibiotic formulations, have biocontrol effect and good stability preferably.As the jinggangmeisu of having succeeded in developing is the kind of output and consumption maximum in China's microbiotic.Be used to prevent and treat rice sheath blight disease, false smut more.Be used for vegetable sprout term damping-off and white thin,tough silk etc. on the vegetables more.Also can be used for preventing and treating wheat hypochnus.Preparation has jingganmycin water solube powder, aqua, pulvis.In addition, to people and animals' low toxicity, to fish, honeybee safety.
Isolating plant endogenesis epiphyte sociales ball hair shell Chaetomium globosumND35=spiral hair shell C.spirale ND35 from the healthy Cortex Populi Tomentosae, in the bacteriostatic test of face-off cultivation and secondary metabolite that exsomatizes, show and plant soil to more than 20 and pass, plant the plant pathogenic fungis that pass gentle biography in various degree restraining effect is arranged, its mechanism of action is except the hyperparasitism to some phytopathogens, and main is antibiosis.Plant endogenesis epiphyte ball hair shell has wide spectrum antagonism and strong resistance, demonstrate the inducing anti-disease effect to tomato, Kidney bean gray mold in the greenhouse, also showing in field biological and ecological methods to prevent plant disease, pests, and erosion test has good prevention effect to the rotten mould root rot of cucumber, the curved mould leaf spot of spore of corn and apple tree canker.Test verified, the antibiotics crude extract that plant endogenesis epiphyte chaetomium globosum ND35 produces is than the secondary metabolite of single component, can more effectively prevent and treat the various plants fungal disease, and more stable under high temperature and sour environment condition, shown its biological and ecological methods to prevent plant disease, pests, and erosion potential.Therefore, development and use hair shell antibiotics controlling plant diseases is to improve the reliable method of preventive effect and stability.
(3) summary of the invention
What the objective of the invention is that method by fermentation culture and chemical extraction obtains that plant endogenesis epiphyte chaetomium globosum ND35 produces has an active antibiotics of broad-spectrum antibacterial, is used for the biological control of Plant diseases.
Gordian technique of the present invention and process are that the pure growth-mycelia cake with fresh plant endogenesis epiphyte chaetomium globosum ND35 is inoculated in Semen Maydis powder or the potato sucrose liquid nutrient medium, under 28 ℃ of conditions, and with 160 rev/mins, shaking culture 15 days.Fermented liquid filters out mycelia with double gauze, under 20 ℃ of conditions, 8000 rev/mins, centrifugal 15 minutes, gets supernatant liquor and obtains cultivating filtered liquid then.Cultivate filtered liquid equal volume of ethyl acetate 3 times.Extraction liquid is used filter paper filtering again behind anhydrous sodium sulfate drying.Under 30 ℃, 140 rev/mins of rotary evaporations return molten collection with small amount of acetone again, treat acetone volatilization after, make tawny solid antibiotics.
The present invention develops and has prepared the antibiotics that plant endogenesis epiphyte ball hair shell ND35 produces, this antibiotics has efficient and broad-spectrum antifungal activity after tested, kind of plant pathogenic fungi surplus 20 there is restraining effect, can be used for the biological control of silborne fungal diseases, the dried fungal disease of woods fruit branch, vegetable gardening disease and field crop disease.
(4) description of drawings
Fig. 1: plant endogenesis epiphyte chaetomium globosum ND35 produces the preparation method of antibiotics
Among the figure 1 be the plant endogenesis epiphyte chaetomium globosum ND35 bacterial classification inoculation that will preserve to the potato dextrose agar flat board, cultivated 5 days for 28 ℃; The 2nd, the mycelia cake of the chaetomium globosum ND35 that newly cultivates is inoculated in Semen Maydis powder or the potato sucrose liquid nutrient medium, under 28 ℃ of conditions, with 160 rev/mins, shaking culture 15 days; The 3rd, fermented liquid filters out mycelia and substratum residue with double gauze, under 20 ℃, 8000 rev/mins, centrifugal 15 minutes, gets supernatant liquor and obtains cultivating filtered liquid; The 4th, cultivate filtered liquid equal volume of ethyl acetate 3 times, extraction liquid is used filter paper filtering again behind anhydrous sodium sulfate drying.The 5th, under 30 ℃, 140 rev/mins, the decompression rotary evaporation of bleeding returns molten collection with small amount of acetone, treat acetone volatilization after, make the tawny solid antibiotics that plant endogenesis epiphyte chaetomium globosum ND35 produces.
(5) preferred forms
Bacterial classification prepare (1) aseptic technique with the plant endogenesis epiphyte chaetomium globosum ND35 bacterial classification inoculation preserved to the potato dextrose agar flat board, cultivated 5 days for 28 ℃, visible dense chaetomium aerial hyphae is covered with flat board.
Liquid fermentation and culture (2) is that 5 millimeters punch tool is beaten and got the bacterium cake with diameter under aseptic condition, is inoculated in Semen Maydis powder or the potato sucrose liquid nutrient medium.Under 28 ℃ of conditions, with 160 rev/mins, shaking culture 15 days.
After filtration and centrifugal (3) fermented liquid filter out mycelia and substratum residue with double gauze, under 20 ℃ of conditions, 8000 rev/mins, centrifugal 15 minutes, get supernatant liquor and obtain cultivating filtered liquid.
Chemical extraction (4) is cultivated filtered liquid equal volume of ethyl acetate 3 times, and extraction liquid is used filter paper filtering again behind anhydrous sodium sulfate drying.
Sample concentration and collection (5) acetic acid ethyl acetate extract is at 30 ℃, under 140 rev/mins of conditions, and the decompression rotary evaporation of bleeding, dry-matter returns molten collection bottling with small amount of acetone.After treating the acetone volatilization, make the tawny solid antibiotics that plant endogenesis epiphyte chaetomium globosum ND35 produces.
Claims (1)
1, a kind of plant endogenesis epiphyte chaetomium globosum ND35 produces the preparation method of antibiotics, it is characterized in that at first chaetomium globosum ND35 at first being inoculated in Semen Maydis powder or the potato sucrose liquid nutrient medium, under 28 ℃ of conditions, with 160 rev/mins, shaking culture 15 days, again fermented liquid is filtered out mycelia and substratum residue with double gauze, then under 20 ℃ of conditions, 8000 rev/mins, centrifugal 15 minutes, get supernatant liquor and obtain cultivating filtered liquid, cultivate filtered liquid equal volume of ethyl acetate 3 times, extraction liquid is used filter paper filtering again behind anhydrous sodium sulfate drying, through the decompression of bleeding, under 30 ℃, 140 rev/mins of rotary evaporations return molten collection with small amount of acetone again, after treating the acetone volatilization, make tawny solid antibiotics.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008100153887A CN101280320A (en) | 2008-04-30 | 2008-04-30 | Method for preparing antibiotic substance from chaetomium globosum of plant endophytic fungi |
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| CNA2008100153887A CN101280320A (en) | 2008-04-30 | 2008-04-30 | Method for preparing antibiotic substance from chaetomium globosum of plant endophytic fungi |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102311925A (en) * | 2011-08-03 | 2012-01-11 | 山东农业大学 | Endophytic fungi chaetomium globosum strain, microbial agent and application thereof |
| CN102676398A (en) * | 2012-03-14 | 2012-09-19 | 安徽农业大学 | Separation and purification method of endophytic fungi from ginkgo biloba |
| CN102690759A (en) * | 2012-06-05 | 2012-09-26 | 安徽农业大学 | Separation and purification method of endophytic fungi of solidago canadensis |
| CN102911879A (en) * | 2012-10-25 | 2013-02-06 | 青岛农业大学 | Chaetomium globosum and application thereof |
| CN103184161A (en) * | 2013-03-12 | 2013-07-03 | 山东农业大学 | Biocontrol strain HTC for preventing pepper phytophthora blight and application thereof |
| CN105524841A (en) * | 2015-11-09 | 2016-04-27 | 潍坊学院 | Broad-spectrum chaetomium strain applied to biological control of plant diseases and applications of broad-spectrum chaetomium strain |
| CN106754396A (en) * | 2016-11-16 | 2017-05-31 | 河南省农业科学院 | A kind of Chinese toon endogenetic fungus TS8 and its secondary metabolite, preparation method and application |
| CN107136123A (en) * | 2017-04-19 | 2017-09-08 | 明光市农源农作物专业合作社 | A kind of sodium alginate for preventing and treating clubroot is coated bacteria agent and preparation method thereof |
| CN113862162A (en) * | 2021-11-11 | 2021-12-31 | 河南省农业科学院植物保护研究所 | Chaetomium globosum and application thereof in preparation of medicament for preventing and treating wheat sharp eyespot |
-
2008
- 2008-04-30 CN CNA2008100153887A patent/CN101280320A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102311925A (en) * | 2011-08-03 | 2012-01-11 | 山东农业大学 | Endophytic fungi chaetomium globosum strain, microbial agent and application thereof |
| CN102676398B (en) * | 2012-03-14 | 2014-01-15 | 安徽农业大学 | Separation and purification method of endophytic fungi from ginkgo biloba |
| CN102676398A (en) * | 2012-03-14 | 2012-09-19 | 安徽农业大学 | Separation and purification method of endophytic fungi from ginkgo biloba |
| CN102690759A (en) * | 2012-06-05 | 2012-09-26 | 安徽农业大学 | Separation and purification method of endophytic fungi of solidago canadensis |
| CN102911879A (en) * | 2012-10-25 | 2013-02-06 | 青岛农业大学 | Chaetomium globosum and application thereof |
| CN102911879B (en) * | 2012-10-25 | 2013-08-21 | 青岛农业大学 | Chaetomium globosum and application thereof |
| CN103184161A (en) * | 2013-03-12 | 2013-07-03 | 山东农业大学 | Biocontrol strain HTC for preventing pepper phytophthora blight and application thereof |
| CN103184161B (en) * | 2013-03-12 | 2015-04-22 | 山东农业大学 | Biocontrol strain HTC for preventing pepper phytophthora blight and application thereof |
| CN105524841A (en) * | 2015-11-09 | 2016-04-27 | 潍坊学院 | Broad-spectrum chaetomium strain applied to biological control of plant diseases and applications of broad-spectrum chaetomium strain |
| CN106754396A (en) * | 2016-11-16 | 2017-05-31 | 河南省农业科学院 | A kind of Chinese toon endogenetic fungus TS8 and its secondary metabolite, preparation method and application |
| CN106754396B (en) * | 2016-11-16 | 2019-08-16 | 河南省农业科学院 | A kind of Chinese toon endogenetic fungus TS8 and its secondary metabolite, preparation method and application |
| CN107136123A (en) * | 2017-04-19 | 2017-09-08 | 明光市农源农作物专业合作社 | A kind of sodium alginate for preventing and treating clubroot is coated bacteria agent and preparation method thereof |
| CN113862162A (en) * | 2021-11-11 | 2021-12-31 | 河南省农业科学院植物保护研究所 | Chaetomium globosum and application thereof in preparation of medicament for preventing and treating wheat sharp eyespot |
| CN113862162B (en) * | 2021-11-11 | 2023-03-28 | 河南省农业科学院植物保护研究所 | Chaetomium globosum and application thereof in preparation of medicament for preventing and treating wheat sharp eyespot |
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Open date: 20081008 |