CN101664046B - Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof - Google Patents
Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof Download PDFInfo
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- CN101664046B CN101664046B CN 200910095000 CN200910095000A CN101664046B CN 101664046 B CN101664046 B CN 101664046B CN 200910095000 CN200910095000 CN 200910095000 CN 200910095000 A CN200910095000 A CN 200910095000A CN 101664046 B CN101664046 B CN 101664046B
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Abstract
The invention discloses a stewartia sinensis herba lycopi vinegar liquid and a preparation method thereof, belonging to the technical field of development and research of plant source pesticide. The preparation method of the stewartia sinensis herba lycopi vinegar liquid comprises the following steps: cutting stewartia sinensis herba lycopi into short sections, adding the sections into a dry distillation kettle, heating the dry distillation kettle, condensing, collecting all distillates at the temperature of 90-300 DEG C and collecting liquid of the stewartia sinensis herba lycopi vinegar liquid of the invention at 12h+/-20min. The stewartia sinensis herba lycopi vinegar liquid has an effective nematode-killing activity against plant nematodes, and the plant nematode killing effect of liquid with 25-150 times of the vinegar liquid can reach 70-100%. The preparation method has simple technique; and compared with methods of extracting by organic solvents, the preparation method has the advantages of lower cost and environment protection.
Description
Technical field
The present invention relates to a kind of Stewartia sinensis herba lycopi vinegar liquid of producing through pyrolysis take Eupatorium adenophorum as raw material and the preparation method, platymiscium source pesticide developmental research technical field.
Background technology
Eupatorium adenophorum (Eupatorium adenophorum Spreng) grows because of the other plant of invading farmland, forest land etc. and just suppressing around it, the single advantage of very fast formation group, thereby a kind of pest that becomes in China, caused huge economic loss.Because this destructiveness of Eupatorium adenophorum, thoroughly eradicating is the best precautionary measures, but in the area that serious harm occurs, its potential value is researched and developed, and also is the important measures that reduce its negative effect.
Along with the continuous research to Eupatorium adenophorum, people also increase gradually to the aspect that utilizes of Eupatorium adenophorum, and have brought certain economic benefit and value.Up to the present, the scientific worker mainly is to use solvent to extract active substance by apparatus,Soxhlet's, Rotary Evaporators etc. to the activity research of Eupatorium adenophorum, then research and utilization in addition, its extraction process is loaded down with trivial details, and cost of investment is large, and organic reagent also can work the mischief to human body and environment.
By literature search, have no the open report identical with the present invention.
Summary of the invention
The technical problem to be solved in the present invention is to overcome also not have the Stewartia sinensis herba lycopi vinegar liquid product in the prior art, it is loaded down with trivial details to extract Eupatorium adenophorum active substance technique, cost of investment is large, the defective that extraction process works the mischief to human body and environment with organic reagent, a kind of Stewartia sinensis herba lycopi vinegar liquid and easy preparation method thereof are provided, and this Stewartia sinensis herba lycopi vinegar liquid has the activity of efficient plant nematocide.
For solving the problems of the technologies described above, technical scheme of the present invention is as follows:
One, Stewartia sinensis herba lycopi vinegar liquid, described Stewartia sinensis herba lycopi vinegar liquid prepares with following method:
A. Eupatorium adenophorum (Eupatorium adenophorum Spreng) is cut into segment, adds in the retort 1;
B. add the sealing ring on the retort 1, installed the lid of retort 1, tightened its bolt;
C. open the drain tap 3 on the retort 1;
D. open heater 2, retort 1 is heated;
E. when thermometer 17 reaches 90 ℃-100 ℃, close drain tap 3;
F. open chilled water valve 12, open the wireway valve 4 on the retort 1;
G. the temperature of control heater 2 under the normal pressure, utilizes thermometer 16 to measure the temperature of retort 1, and 90 ℃～300 ℃ all cuts are collected in condensation;
H. when 12h ± 20min, close successively the wireway valve 4 on the retort 1, open drain tap 3, close heater 2;
I. close chilled water valve 12, take off flask with three necks,round bottom 9, the liquid that flask with three necks,round bottom 9 is collected is described Stewartia sinensis herba lycopi vinegar liquid, is placed in the brown bottle to keep.
Two, the preparation method of above-mentioned Stewartia sinensis herba lycopi vinegar liquid provided by the invention, carry out according to the following steps:
A. Eupatorium adenophorum (Eupatorium adenophorum Spreng) is cut into segment, adds in the retort 1;
B. add the sealing ring on the retort 1, installed the lid of retort 1, tightened its bolt;
C. open the drain tap 3 on the retort 1;
D. open heater 2, retort 1 is heated;
E. when thermometer 17 reaches 90 ℃-100 ℃, close drain tap 3;
F. open chilled water valve 12, open the wireway valve 4 on the retort 1;
G. the temperature of control heater 2 under the normal pressure, utilizes thermometer 16 to measure the temperature of retort 1, and 90 ℃～300 ℃ all cuts are collected in condensation;
H. when 12h ± 20min, close successively the wireway valve 4 on the retort 1, open drain tap 3, close heater 2;
I. close chilled water valve 12, take off flask with three necks,round bottom 9, the liquid that flask with three necks,round bottom 9 is collected is described Stewartia sinensis herba lycopi vinegar liquid, is placed in the brown bottle to keep.
The invention has the beneficial effects as follows:
1, use Stewartia sinensis herba lycopi vinegar liquid of the present invention, Plant nematode is had efficient eelworm-killing activity, the plant nematocide effect of 25 times of liquid-150 times liquid of its vinegar liquid can reach 70%-100%.
2, the preparation method of Stewartia sinensis herba lycopi vinegar liquid of the present invention, technique is simple, compares with an organic solvent extraction, and cost is lower, the problem of environmental pollution that does not with an organic solvent bring.
Description of drawings:
Fig. 1 is the equipment schematic diagram of preparation Stewartia sinensis herba lycopi vinegar liquid of the present invention, and each mark represents successively among its figure: the 1-retort; The 2-heater; The 3-drain tap; 4-wireway valve; 5-condenser pipe air induction conduit; The 6-condenser pipe; 7-condenser pipe delivery port; The exhaust manifolds of 8-flask with three necks,round bottom; The 9-flask with three necks,round bottom; The 10-round-bottomed flask; 11-condensate draining delivery port; 12-chilled water valve; 13-condenser pipe water inlet pipe; The 14-condensate draining; The liquid inlet of 15-flask with three necks,round bottom; The 16-thermometer.
Embodiment:
The invention will be further described below in conjunction with embodiment, but be not construed as limiting the present invention.
Example 1 Stewartia sinensis herba lycopi vinegar liquid of the present invention and preparation method thereof
Stewartia sinensis herba lycopi vinegar liquid of the present invention makes by the equipment of Fig. 1, equipment shown in Figure 1 is the vinegar liquid easy preparation device that utilizes the routine of destructive distillation condensation principle design, each parts by the routine of Laboratory Instruments connect, installation method connects and install, wherein fill distilled water in the round-bottomed flask 10, uncooled tail gas is carried out purification filtering.
Below the Latin name of used Eupatorium adenophorum plant be Eupatorium adenophorum Spreng.
(1) Stewartia sinensis herba lycopi vinegar liquid of the present invention, described Stewartia sinensis herba lycopi vinegar liquid prepares with following method:
A. Eupatorium adenophorum is cut into segment, adds in the retort 1;
B. add the sealing ring on the retort 1, installed the lid of retort 1, tightened its bolt;
C. open the drain tap 3 on the retort 1;
D. open heater 2, retort 1 is heated;
E. when thermometer 17 reaches 90 ℃-100 ℃, close drain tap 3;
F. open chilled water valve 12, open the wireway valve 4 on the retort 1;
G. the temperature of control heater 2 under the normal pressure, utilizes thermometer 16 to measure the temperature of retort 1, and 90 ℃～300 ℃ all cuts are collected in condensation;
H. when 12h ± 20min, close successively the wireway valve 4 on the retort 1, open drain tap 3, close heater 2;
I. close chilled water valve 12, take off flask with three necks,round bottom 9, the liquid that flask with three necks,round bottom 9 is collected is described Stewartia sinensis herba lycopi vinegar liquid, is placed in the brown bottle to keep.
(2) preparation method of Stewartia sinensis herba lycopi vinegar liquid of the present invention, carry out according to the following steps:
A. Eupatorium adenophorum is cut into segment, adds in the retort 1;
B. add the sealing ring on the retort 1, installed the lid of retort 1, tightened its bolt;
C. open the drain tap 3 on the retort 1;
D. open heater 2, retort 1 is heated;
E. when thermometer 17 reaches 90 ℃-100 ℃, close drain tap 3;
F. open chilled water valve 12, open the wireway valve 4 on the retort 1;
G. the temperature of control heater 2 under the normal pressure, utilizes thermometer 16 to measure the temperature of retort 1, and 90 ℃～300 ℃ all cuts are collected in condensation;
H. when 12h ± 20min, close successively the wireway valve 4 on the retort 1, open drain tap 3, close heater 2;
I. close chilled water valve 12, take off flask with three necks,round bottom 9, the liquid that flask with three necks,round bottom 9 is collected is described Stewartia sinensis herba lycopi vinegar liquid, is placed in the brown bottle to keep.
Embodiment 2 Stewartia sinensis herba lycopi vinegar liquids of the present invention are to the eelworm-killing activity test of pine wood nematode
Test reagent: with the Stewartia sinensis herba lycopi vinegar liquid 150ml of above-described embodiment 1, by 6 dilution gradients that arrange, be mixed with the obtain solution of 25 times of liquid, 50 times of liquid, 75 times of liquid, 100 times of liquid, 150 times of liquid, 200 times of liquid.
Test method: select high-quality transparent surface ware (diameter 60mm), add pine wood nematode (Bursaphelenchus xylophilus) suspension 100 μ l (300/ml) with liquid-transfering gun in each surface plate, in surface plate, add 2ml Stewartia sinensis herba lycopi vinegar liquid obtain solution again, carry out soaking solution in the correction surface ware with stoste, surface plate is placed culture dish, be put in 26 ℃ of constant incubators and cultivate.At 12h, 24h, 48h, 72h surface plate is placed respectively and observe the pine wood nematode survival condition under the stereomicroscope.In clear water, bring back to life 2h and survival condition is counted.Calculate the death toll of pine wood nematode under the different dilution factors of Stewartia sinensis herba lycopi vinegar liquid, irresuscitablely be considered as death.By following Abbott formula calculation correction lethality.
Nematode death is stiff motionless as dead worm take pine wood nematode, and pine wood nematode is crooked wriggling state and is the worm that lives, and the pine wood nematode that bending is lost mobility carries out the life or death that needle point method is judged nematode.
Result of the test: the Stewartia sinensis herba lycopi vinegar liquid of different diluted concentrations all has inhibition to pine wood nematode, but along with the increase of extension rate, inhibition reduces.Under same extension rate, along with the inhibition of the prolongation Stewartia sinensis herba lycopi vinegar liquid of time is being strengthened (table 1) gradually.
Table 1 Stewartia sinensis herba lycopi vinegar liquid is to the eelworm-killing activity of pine wood nematode
Embodiment 3 Stewartia sinensis herba lycopi vinegar liquids of the present invention are to the eelworm-killing activity test of Meloidogyne incognita
Except in embodiment 2 described each surface plate, adding Meloidogyne incognita (Meloidogyne incognita) 2 instar larvae suspensions 100 μ l (300/ml) with liquid-transfering gun, all the other test methods and test reagent, 6 dilution gradients that reagent arranges etc. are all identical with embodiment 2, do not giving unnecessary details.
Result of the test: the Stewartia sinensis herba lycopi vinegar liquid of different diluted concentrations all has inhibition to Meloidogyne incognita 2 instar larvaes, but along with the increase of extension rate, inhibition reduces.Under same extension rate, along with the inhibition of the prolongation Stewartia sinensis herba lycopi vinegar liquid of time is being strengthened (table 2) gradually.
Table 2 Stewartia sinensis herba lycopi vinegar liquid is to the eelworm-killing activity of Meloidogyne incognita
Above-mentioned test shows that Stewartia sinensis herba lycopi vinegar liquid of the present invention has efficient eelworm-killing activity to plant pathogeny line insect.
Claims (2)
1. Stewartia sinensis herba lycopi vinegar liquid, described Stewartia sinensis herba lycopi vinegar liquid prepares with following method:
A. the Eupatorium adenophorum that with Latin name is Eupatorium adenophorum Spreng is cut into segment, adds in the retort (1);
B. add the sealing ring on the retort (1), installed the lid of retort (1), tightened its bolt;
C. open the drain tap (3) on the retort (1);
D. open heater (2), retort (1) is heated;
E. when thermometer (16) reaches 90 ℃-100 ℃, close drain tap (3);
F. open chilled water valve (12), open the wireway valve (4) on the retort (1);
G. the temperature of control heater (2) under the normal pressure, utilizes thermometer (16) to measure the temperature of retort (1), and 90 ℃～300 ℃ all cuts are collected in condensation;
H. when 12h ± 20min, close successively the wireway valve (4) on the retort (1), open drain tap (3), close heater (2);
I. close chilled water valve (12), take off flask with three necks,round bottom (9), the liquid that flask with three necks,round bottom (9) is collected is described Stewartia sinensis herba lycopi vinegar liquid, is placed in the brown bottle to keep.
2. the method for preparing Stewartia sinensis herba lycopi vinegar liquid claimed in claim 1, carry out according to the following steps:
A. the Eupatorium adenophorum that with Latin name is Eupatorium adenophorum Spreng is cut into segment, adds in the retort (1);
B. add the sealing ring on the retort (1), installed the lid of retort (1), tightened its bolt;
C. open the drain tap (3) on the retort (1);
D. open heater (2), retort (1) is heated;
E. when thermometer (16) reaches 90 ℃-100 ℃, close drain tap (3);
F. open chilled water valve (12), open the wireway valve (4) on the retort (1);
G. the temperature of control heater (2) under the normal pressure, utilizes thermometer (16) to measure the temperature of retort (1), and 90 ℃～300 ℃ all cuts are collected in condensation;
H. when 12h ± 20min, close successively the wireway valve (4) on the retort (1), open drain tap (3), close heater (2);
I. close chilled water valve (12), take off flask with three necks,round bottom (9), the liquid that flask with three necks,round bottom (9) is collected is described Stewartia sinensis herba lycopi vinegar liquid, is placed in the brown bottle to keep.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910095000 CN101664046B (en) | 2009-09-23 | 2009-09-23 | Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910095000 CN101664046B (en) | 2009-09-23 | 2009-09-23 | Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof |
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| Publication Number | Publication Date |
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| CN101664046A CN101664046A (en) | 2010-03-10 |
| CN101664046B true CN101664046B (en) | 2013-01-30 |
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| CN 200910095000 Expired - Fee Related CN101664046B (en) | 2009-09-23 | 2009-09-23 | Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107372633B (en) * | 2017-08-11 | 2020-04-21 | 云南中医学院 | Application of artemisia annua tobacco vinegar liquid in inhibition of root-knot nematodes |
| CN107372634B (en) * | 2017-08-11 | 2020-04-21 | 云南中医学院 | Artemisia annua tobacco vinegar liquid |
| CN111838195A (en) * | 2020-07-23 | 2020-10-30 | 王婉君 | Novel environment-friendly plant source artemisia annua tobacco vinegar liquid for agriculture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1788570A (en) * | 2005-12-27 | 2006-06-21 | 云南农业大学 | Purple stem boneset extract for prevention and treatment of plant disease |
| CN101011070A (en) * | 2006-08-09 | 2007-08-08 | 云南师范大学 | Method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice |
| CN101317588A (en) * | 2007-06-08 | 2008-12-10 | 中国农业科学院植物保护研究所 | Application of eupatorium adenophorum extracting solution as plant source pesticide |
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2009
- 2009-09-23 CN CN 200910095000 patent/CN101664046B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1788570A (en) * | 2005-12-27 | 2006-06-21 | 云南农业大学 | Purple stem boneset extract for prevention and treatment of plant disease |
| CN101011070A (en) * | 2006-08-09 | 2007-08-08 | 云南师范大学 | Method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice |
| CN101317588A (en) * | 2007-06-08 | 2008-12-10 | 中国农业科学院植物保护研究所 | Application of eupatorium adenophorum extracting solution as plant source pesticide |
Non-Patent Citations (2)
| Title |
|---|
| 竹炭的加工与利用;谭宏超等;《云南林业》;20040715;第25卷(第4期);21-22 * |
| 谭宏超等.竹炭的加工与利用.《云南林业》.2004,第25卷(第4期), |
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