CN101230393A - 检棒分析中改良的结合相互作用 - Google Patents
检棒分析中改良的结合相互作用 Download PDFInfo
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- CN101230393A CN101230393A CNA2007101849913A CN200710184991A CN101230393A CN 101230393 A CN101230393 A CN 101230393A CN A2007101849913 A CNA2007101849913 A CN A2007101849913A CN 200710184991 A CN200710184991 A CN 200710184991A CN 101230393 A CN101230393 A CN 101230393A
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- nucleic acid
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measurement Of Length, Angles, Or The Like Using Electric Or Magnetic Means (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0016836.9 | 2000-07-07 | ||
| GBGB0016836.9A GB0016836D0 (en) | 2000-07-07 | 2000-07-07 | Improved dipstick assays (1) |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB018153097A Division CN100355905C (zh) | 2000-07-07 | 2001-07-06 | 检棒分析中改良的结合相互作用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101230393A true CN101230393A (zh) | 2008-07-30 |
Family
ID=9895306
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007101849913A Pending CN101230393A (zh) | 2000-07-07 | 2001-07-06 | 检棒分析中改良的结合相互作用 |
| CNB018153097A Expired - Lifetime CN100355905C (zh) | 2000-07-07 | 2001-07-06 | 检棒分析中改良的结合相互作用 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB018153097A Expired - Lifetime CN100355905C (zh) | 2000-07-07 | 2001-07-06 | 检棒分析中改良的结合相互作用 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US8431336B2 (enExample) |
| EP (1) | EP1325151B1 (enExample) |
| JP (2) | JP5341291B2 (enExample) |
| CN (2) | CN101230393A (enExample) |
| AT (1) | ATE521714T1 (enExample) |
| AU (2) | AU6928501A (enExample) |
| ES (1) | ES2372477T3 (enExample) |
| GB (1) | GB0016836D0 (enExample) |
| WO (1) | WO2002004671A2 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0016813D0 (en) * | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (4) |
| GB0016836D0 (en) | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (1) |
| GB0025245D0 (en) | 2000-10-14 | 2000-11-29 | Lee Helen | Multiple target detection |
| DE10154291B4 (de) * | 2001-11-05 | 2005-05-19 | Hain Lifescience Gmbh | Verfahren zum Nachweis von Nukleinsäuren in Form eines Trockenschnelltestes |
| US8669052B2 (en) | 2008-06-10 | 2014-03-11 | Rapid Pathogen Screening, Inc. | Lateral flow nucleic acid detector |
| GB0701253D0 (en) | 2007-01-23 | 2007-02-28 | Diagnostics For The Real World | Nucleic acid amplification and testing |
| US20110086359A1 (en) * | 2008-06-10 | 2011-04-14 | Rapid Pathogen Screening, Inc. | Lateral flow assays |
| JP2012503170A (ja) * | 2008-07-15 | 2012-02-02 | ラピッド パトゲン スクリーニング，インク． | 側方流動イムノアッセイにおけるインサイツでの細胞の溶解 |
| EP2438184B1 (en) * | 2009-06-02 | 2014-06-04 | Monoquant PTY LTD | A method of nucleic acid amplification |
| WO2012070618A1 (ja) | 2010-11-24 | 2012-05-31 | 株式会社カネカ | 増幅核酸検出方法及び検出デバイス |
| JP2013198482A (ja) * | 2012-02-22 | 2013-10-03 | Nippon Meat Packers Inc | 核酸の検出方法 |
| WO2014007289A1 (ja) * | 2012-07-05 | 2014-01-09 | 日本碍子株式会社 | 核酸クロマトグラフィー法、核酸クロマトグラフィー用組成物及びこれを含むキット |
| WO2015138590A1 (en) | 2014-03-11 | 2015-09-17 | Bio-Rad Laboratories, Inc. | Dip-stick western blot |
| JP6734011B2 (ja) * | 2014-06-06 | 2020-08-05 | 古河電気工業株式会社 | 生体分子検出用試験キット、及びこれを用いた生体分子の検出方法、並びにこれらに用いられる生体分子検出用試験片及び生体分子検出用標識試薬 |
| CN108026581A (zh) | 2015-06-19 | 2018-05-11 | 剑桥企业有限公司 | 感染性疾病的诊断与治疗 |
| WO2018180987A1 (ja) * | 2017-03-29 | 2018-10-04 | 東洋紡株式会社 | 核酸の検出方法 |
| CN114040983A (zh) * | 2019-06-26 | 2022-02-11 | 南京金斯瑞生物科技有限公司 | 一种含有阻断物的寡核苷酸 |
| MX2020006374A (es) * | 2020-07-13 | 2022-01-14 | Univ Mexico Nac Autonoma | Metodo de hibridacion para la deteccion de acidos nucleicos empleando particulas magneticas funcionalizadas. |
Family Cites Families (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1223222A (en) * | 1984-02-22 | 1987-06-23 | Nanibhushan Dattagupta | Immobilized nucleic acid-containing probes |
| CA1253777A (en) | 1984-06-01 | 1989-05-09 | Robert J. Carrico | Nucleic acid hybridization assay employing immobilized rna probes |
| EP0187332B1 (en) | 1985-01-10 | 1989-02-01 | Molecular Diagnostics, Inc. | Photochemical method of labelling nucleic acids for detection in hybridization assays |
| GB8509367D0 (en) | 1985-04-12 | 1985-05-15 | Amersham Int Plc | Nucleic acid hybridisation |
| US4751177A (en) * | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
| US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| US4822731A (en) | 1986-01-09 | 1989-04-18 | Cetus Corporation | Process for labeling single-stranded nucleic acids and hybridizaiton probes |
| US5317098A (en) | 1986-03-17 | 1994-05-31 | Hiroaki Shizuya | Non-radioisotope tagging of fragments |
| US5310650A (en) * | 1986-09-29 | 1994-05-10 | Abbott Laboratoires | Method and device for improved reaction kinetics in nucleic acid hybridizations |
| US5283174A (en) * | 1987-09-21 | 1994-02-01 | Gen-Probe, Incorporated | Homogenous protection assay |
| AU2714988A (en) | 1987-10-23 | 1989-06-01 | Siska Diagnostics, Inc. | Lanthanide chelate-tagged nucleic acid probes |
| WO1990001564A1 (en) * | 1988-08-09 | 1990-02-22 | Microprobe Corporation | Methods for multiple target analyses through nucleic acid hybridization |
| GB8822228D0 (en) * | 1988-09-21 | 1988-10-26 | Southern E M | Support-bound oligonucleotides |
| DE3842700A1 (de) * | 1988-12-19 | 1990-06-21 | Boehringer Mannheim Gmbh | Verfahren zur proteinimmobilisierung an einer festphase, so hergestellte protein tragende festphase sowie deren verwendung |
| ATE157404T1 (de) | 1989-03-17 | 1997-09-15 | Abbott Lab | Verfahren und vorrichtung zur hybridisierung von nukleinsäure mit verbesserter reaktionskinetik |
| ATE174926T1 (de) * | 1989-06-30 | 1999-01-15 | Chiron Corp | Hydrophobe nukleinsäure-sonde |
| US5232829A (en) | 1989-09-29 | 1993-08-03 | Hoffmann-La Roche Inc. | Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes |
| ATE152777T1 (de) | 1989-12-04 | 1997-05-15 | Microprobe Corp | Verstärkter einfang von ziel-nukleinsäuren mittels verwendung von oligonukleotiden, die kovalent an polymere gebunden sind |
| CA2031659A1 (en) * | 1990-01-26 | 1991-07-27 | John B. Findlay | Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods |
| FR2663040B1 (fr) * | 1990-06-11 | 1995-09-15 | Bio Merieux | Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich. |
| US5695926A (en) | 1990-06-11 | 1997-12-09 | Bio Merieux | Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5663318A (en) * | 1991-03-01 | 1997-09-02 | Pegg; Randall Kevin | Assay preparation containing capture and detection polynucleotides covalently bound to substrates with a heterobifunctional crosslinking agent |
| FR2679255B1 (fr) | 1991-07-17 | 1993-10-22 | Bio Merieux | Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation. |
| US5976789A (en) | 1991-07-17 | 1999-11-02 | Bio Merieux | System of probes enabling HLA-DR typing to be performed, and typing method using said probes |
| FR2679252B1 (fr) | 1991-07-17 | 1993-12-10 | Bio Merieux | Systeme de sondes permettant d'effectuer le typage hla dr, et procede de typage utilisant lesdites sondes. |
| US5747244A (en) * | 1991-12-23 | 1998-05-05 | Chiron Corporation | Nucleic acid probes immobilized on polystyrene surfaces |
| AU4544193A (en) | 1992-06-25 | 1994-01-24 | Microprobe Corporation | Solid supports for nucleic acid hybridization assays |
| DE69327326T2 (de) * | 1992-07-24 | 2001-08-16 | Diatech Pty. Ltd., Brisbane | Vervielfältigungs- und detektionsprozess |
| US5438131A (en) | 1992-09-16 | 1995-08-01 | Bergstrom; Donald E. | 3-nitropyrrole nucleoside |
| AU4925993A (en) | 1992-09-18 | 1994-04-12 | Abbott Laboratories | Multiple assay test strip devices |
| WO1994012670A2 (en) | 1992-11-27 | 1994-06-09 | N.V. Innogenetics S.A. | Process for typing of hcv isolates |
| WO1994023299A1 (en) | 1993-03-31 | 1994-10-13 | Quidel Corporation | Multiple assay device |
| US5496934A (en) * | 1993-04-14 | 1996-03-05 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Nucleic acids encoding a cellulose binding domain |
| EP0705426A4 (en) | 1993-06-09 | 1998-07-08 | Quidel Corp | SPECIFIC ONE-STEP TITRATIONS OF AN ANTIGEN |
| US5635602A (en) * | 1993-08-13 | 1997-06-03 | The Regents Of The University Of California | Design and synthesis of bispecific DNA-antibody conjugates |
| WO1995005391A1 (en) | 1993-08-18 | 1995-02-23 | Chromagen, Inc. | Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides |
| CA2170264A1 (en) * | 1993-09-10 | 1995-03-16 | Michael W. Konrad | Optical detection of position of oligonucleotides on large dna molecules |
| US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| DE4406524A1 (de) * | 1994-02-28 | 1995-08-31 | Boehringer Mannheim Gmbh | 3'-RNA-Markierung mit Terminaler Transferase |
| WO1995027081A1 (en) * | 1994-03-31 | 1995-10-12 | E.I. Du Pont De Nemours And Company | A method for detection of nucleic acid fragments |
| US5747248A (en) | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
| ES2205030T3 (es) | 1995-05-09 | 2004-05-01 | Kreatech Biotechnology B.V. | Procedimientos para la produccion de segmentos de conexion a base de platino entre marcadores y moleculas bio-organicas, para el marcado de moleculas bio-organicas, la deteccion de sustancias biologicas a estudiar. |
| DE19537952A1 (de) | 1995-10-12 | 1997-04-17 | Boehringer Mannheim Gmbh | Verfahren zum Nachweis eines Analyten |
| US5874216A (en) * | 1996-02-23 | 1999-02-23 | Ensys Environmental Products, Inc. | Indirect label assay device for detecting small molecules and method of use thereof |
| WO1998015564A1 (en) | 1996-10-08 | 1998-04-16 | Kreatech Biotechnology B.V. | Methods for labeling nucleotides, labeled nucleotides and useful intermediates |
| CA2323638A1 (en) * | 1998-04-03 | 1999-10-14 | Phylos, Inc. | Addressable protein arrays |
| US20020012913A1 (en) | 1998-09-15 | 2002-01-31 | Kevin L. Gunderson | Nucleic acid analysis using complete n-mer arrays |
| AU1681000A (en) | 1998-12-10 | 2000-06-26 | Takara Shuzo Co., Ltd. | Method for immobilizing oligonucleotide on a carrier |
| DE19901761A1 (de) | 1999-01-18 | 1999-07-01 | Gerhard Dr Hartwich | Verfahren zur elektrochemischen Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen |
| DE19910761A1 (de) * | 1999-03-11 | 2000-09-14 | Tellmann Wilfried | Magnetmotor |
| WO2000061806A2 (en) | 1999-04-09 | 2000-10-19 | Arcturus Engineering, Inc. | GENERIC cDNA OR PROTEIN ARRAY FOR CUSTOMIZED ASSAYS |
| GB0016814D0 (en) | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (3) |
| GB0016833D0 (en) | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (2) |
| GB0016836D0 (en) | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (1) |
| GB0025245D0 (en) | 2000-10-14 | 2000-11-29 | Lee Helen | Multiple target detection |
| GB0029154D0 (en) | 2000-11-30 | 2001-01-17 | Lee Helen | Signal enhancement with multiple labelled-antibodies |
| GB0130947D0 (en) | 2001-12-24 | 2002-02-13 | Lee Helen | Improved sample preparation for the detection of infectious agents |
| GB0217390D0 (en) | 2002-07-26 | 2002-09-04 | Lee Helen | Liquid sampler and method |
-
2000
- 2000-07-07 GB GBGB0016836.9A patent/GB0016836D0/en not_active Ceased
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2001
- 2001-07-06 US US10/332,142 patent/US8431336B2/en not_active Expired - Fee Related
- 2001-07-06 AU AU6928501A patent/AU6928501A/xx active Pending
- 2001-07-06 WO PCT/GB2001/003039 patent/WO2002004671A2/en not_active Ceased
- 2001-07-06 CN CNA2007101849913A patent/CN101230393A/zh active Pending
- 2001-07-06 CN CNB018153097A patent/CN100355905C/zh not_active Expired - Lifetime
- 2001-07-06 AU AU2001269285A patent/AU2001269285B2/en not_active Expired
- 2001-07-06 EP EP01947639A patent/EP1325151B1/en not_active Expired - Lifetime
- 2001-07-06 ES ES01947639T patent/ES2372477T3/es not_active Expired - Lifetime
- 2001-07-06 JP JP2002509524A patent/JP5341291B2/ja not_active Expired - Lifetime
- 2001-07-06 AT AT01947639T patent/ATE521714T1/de not_active IP Right Cessation
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2011
- 2011-09-29 JP JP2011215672A patent/JP2012019797A/ja not_active Withdrawn
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2013
- 2013-04-19 US US13/866,891 patent/US20140106347A1/en not_active Abandoned
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| Publication number | Publication date |
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| CN100355905C (zh) | 2007-12-19 |
| EP1325151B1 (en) | 2011-08-24 |
| ATE521714T1 (de) | 2011-09-15 |
| AU2001269285B2 (en) | 2007-08-16 |
| JP2012019797A (ja) | 2012-02-02 |
| JP5341291B2 (ja) | 2013-11-13 |
| US20040072176A1 (en) | 2004-04-15 |
| US8431336B2 (en) | 2013-04-30 |
| WO2002004671A3 (en) | 2003-05-01 |
| JP2004512499A (ja) | 2004-04-22 |
| CN1452664A (zh) | 2003-10-29 |
| GB0016836D0 (en) | 2000-08-30 |
| EP1325151A2 (en) | 2003-07-09 |
| US20140106347A1 (en) | 2014-04-17 |
| AU6928501A (en) | 2002-01-21 |
| WO2002004671A2 (en) | 2002-01-17 |
| ES2372477T3 (es) | 2012-01-20 |
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