MX2020006374A - Metodo de hibridacion para la deteccion de acidos nucleicos empleando particulas magneticas funcionalizadas. - Google Patents
Metodo de hibridacion para la deteccion de acidos nucleicos empleando particulas magneticas funcionalizadas.Info
- Publication number
- MX2020006374A MX2020006374A MX2020006374A MX2020006374A MX2020006374A MX 2020006374 A MX2020006374 A MX 2020006374A MX 2020006374 A MX2020006374 A MX 2020006374A MX 2020006374 A MX2020006374 A MX 2020006374A MX 2020006374 A MX2020006374 A MX 2020006374A
- Authority
- MX
- Mexico
- Prior art keywords
- magnetic particles
- nucleic acids
- functionalized magnetic
- hybridization method
- detecting nucleic
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención está relacionada con un método de hibridación para la detección de ácidos nucleicos, el cual comprende las etapas de: a) extraer una muestra que contiene el material genético que se desea detectar, ya sea, DNA o RNA; b) unir partículas magnéticas a una cadena de oligonucleótidos sintéticos, en donde la superficie de dichas partículas magnéticas se encuentra recubierta con una proteína y dicha cadena de oligonucleótidos sintéticos se encuentran con un marcaje de unión, de tal manera que a la unión de la proteína con el oligonucleótido se le denomina reacción de funcionalización, obteniéndose en esta etapa partículas magnéticas funcionalizadas; c) incubar la muestra que contiene el material a detectar (ácidos nucleicos, ya sea, DNA o RNA) con una sonda de oligonucleótidos de cadena complementaria con un marcaje fluorescente, a esta reacción se le denomina reacción de competencia; y, d) hacer reaccionar las partículas magnéticas funcionalizadas obtenidas en la etapa b) con un par sonda-blanco obtenido en la etapa c), esta etapa se denomina como reacción de detección.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2020006374A MX2020006374A (es) | 2020-07-13 | 2020-07-13 | Metodo de hibridacion para la deteccion de acidos nucleicos empleando particulas magneticas funcionalizadas. |
| PCT/MX2021/050028 WO2022015146A1 (es) | 2020-07-13 | 2021-06-15 | Método de hibridación para la detección de ácidos nucleicos empleando partículas magnéticas funcionalizadas |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2020006374A MX2020006374A (es) | 2020-07-13 | 2020-07-13 | Metodo de hibridacion para la deteccion de acidos nucleicos empleando particulas magneticas funcionalizadas. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2020006374A true MX2020006374A (es) | 2022-01-14 |
Family
ID=79554896
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2020006374A MX2020006374A (es) | 2020-07-13 | 2020-07-13 | Metodo de hibridacion para la deteccion de acidos nucleicos empleando particulas magneticas funcionalizadas. |
Country Status (2)
| Country | Link |
|---|---|
| MX (1) | MX2020006374A (es) |
| WO (1) | WO2022015146A1 (es) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0016836D0 (en) * | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (1) |
-
2020
- 2020-07-13 MX MX2020006374A patent/MX2020006374A/es unknown
-
2021
- 2021-06-15 WO PCT/MX2021/050028 patent/WO2022015146A1/es active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022015146A1 (es) | 2022-01-20 |
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