CN101142884B - A kind of cultivating method of hickory nut rootstock grafted seedlings - Google Patents
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Abstract
本发明公开了一种山核桃本砧嫁接苗的培育方法,其步骤如下:山核桃本砧苗圃的建立;以化香、山核桃、美国山核桃或湖南山核桃为砧木,以山核桃成年树生长枝为接穗培育采穗母本树;选取留床或在嫁接前一年11月初移植的地径≥0.7cm、健壮的1~2年生山核桃播种苗为砧木,株行距不小于12×25cm;于上年的11月10日至当年的1月31日之间,从采穗圃接穗母本树上采集健壮、芽饱满、节间短的1~2年生生长枝为接穗,采集后在阴暗处放置12~36小时,再用塑料薄膜密封包裹,置于4℃冷库储藏;当苗圃或移植地内30%砧木苗开始萌芽展叶时,于2~3周内完成嫁接。本技术方案可实现山核桃树体矮化、缩短童期、提早结果。The invention discloses a method for cultivating grafted seedlings of hickory root stock. The steps are as follows: establishment of a hickory root stock nursery; taking Huaxiang, hickory, American hickory or Hunan hickory as rootstocks, and taking hickory adult trees The growing branch is the scion to cultivate the female parent tree; select the seedlings with a ground diameter of ≥0.7cm and transplanted at the beginning of November of the year before grafting as the rootstock, and the seedlings of hickory nuts that are 1 to 2 years old are used as rootstocks, and the row spacing between plants is not less than 12×25cm ; Between November 10th of last year and January 31st of the current year, the 1-2 year-old growth branches that are strong, full of buds, and short in internodes are collected from the scion female parent tree in the picking garden as scions. Place it in a dark place for 12-36 hours, then seal and wrap it with plastic film, and store it in a cold storage at 4°C; when 30% of the rootstock seedlings in the nursery or transplantation field begin to germinate and spread leaves, the grafting will be completed within 2-3 weeks. The technical scheme can realize the dwarfing of the hickory tree body, shortening the childhood period, and early fruiting.
Description
技术领域technical field
本发明属于植物的嫁接方法领域,特别是涉及一种山核桃本砧嫁接苗的培育方法。The invention belongs to the field of plant grafting methods, and in particular relates to a method for cultivating hickory tree grafted seedlings.
背景技术Background technique
山核桃(Carya cathayensis)属胡桃科,山核桃属,主产地在浙皖交界的天目山区,因其果实有较高的营养和经济价值,近年来种植面积大大增加。但是,现有山核桃林均是采用野生或播种实生苗就地或移植抚育而成,实生苗造林一般要8年以上才开始结果(野生山核桃12年开始结果),至进入盛果期要20年以后;同时山核桃树干高耸,高的达21米,而产量低下,采收也很不方便;山核桃优良品种类型和单株虽有选择,但由于无性繁殖不过关,难以推广,至今还没有形成品种化。所以,为了提早结果、矮化树体和批量繁殖良种,都必须尽快解决山核桃的无性繁殖技术。Hickory nut (Carya cathayensis) belongs to Juglandaceae, Carya genus, and its main production area is in the Tianmu Mountain area at the junction of Zhejiang and Anhui. Because of its high nutritional and economic value, the planting area has greatly increased in recent years. But existing hickory tree forest all is to adopt wild or sowing seedling on the spot or transplanting and tending to form, and seedling afforestation generally needs more than 8 years just to begin result (wild hickory nut 12 years beginning result), to entering full fruit period and need 20 years later; at the same time, the hickory tree trunk is towering, up to 21 meters high, and the yield is low, and it is very inconvenient to harvest; although there are options for the fine variety types and individual plants of hickory nuts, it is difficult to popularize because of poor asexual reproduction. There is no variety yet. Therefore, in order to bear fruit early, dwarf the tree body and breed fine seeds in batches, the asexual reproduction technology of hickory must be solved as soon as possible.
早在20世纪80年代初开始,浙江林学院、临安县林业局、昌北林业局、临安县林科所和安徽省宣城地区林业局等单位先后进行了山核桃嫁接试验和尝试。但是,利用一般的山核桃成年树枝为接穗,进行本砧嫁接试验成活率均停留在40%以下,而且,虽然可以提早结果却存在新树生长势缓易形成“老头树”的问题,而山核桃成年树以结果枝为主而营养生长枝极少,如作为接穗取走,就会存在老树生长势弱,产量锐减等问题;而利用山核桃苗木枝条为接穗进行本砧嫁接试验成活率可达90%~93%,但是,因为没有达到提早结果、矮化树体和繁殖良种的目的而缺少实用价值;以往也有利用化香(属胡桃科,化香树属)为砧木和山核桃成年树生长枝为接穗嫁接试验成活率为84%~88%,但是由于树种间的不亲和性,到了5-10年后,接穗与砧木之间会自然开裂而死亡,保存率仅为10%以内。As early as the early 1980s, units such as Zhejiang Forestry College, Lin'an County Forestry Bureau, Changbei Forestry Bureau, Lin'an County Forestry Research Institute and Xuancheng District Forestry Bureau of Anhui Province successively carried out hickory grafting experiments and attempts. But, utilize general hickory nut adult tree branch to be scion, carry out this stock grafting test survival rate and all stay below 40%, and, although can bear fruit early, there is the problem that new tree growth vigor is slow and easily forms " old man tree ", and mountain Adult walnut trees are dominated by fruiting branches and very few vegetative growth branches. If they are taken away as scions, there will be problems such as weak growth of old trees and a sharp drop in yield; The rate can reach 90% to 93%, but it lacks practical value because it does not reach the purpose of early fruiting, dwarfing the tree body and breeding good seeds; The growth branch of walnut adult tree is scion grafting test and the survival rate is 84%~88%. However, due to the incompatibility between tree species, after 5-10 years, the scion and rootstock will naturally crack and die, and the preservation rate is only Within 10%.
因此,在本发明方法之前,山核桃的无性繁殖技术仍停留在试验和尝试阶段,还没有形成一种可以大量推广和重复验证的成熟的方法,因此,山核桃树体高大、童期长和适宜作接穗的成年山核桃树生长枝缺少的问题仍旧是严重制约山核桃产业发展的主要难题。因此,如何实现山核桃本砧嫁接育苗是一个发展山核桃产业的关键技术,如果能够成功突破就不但能够解决树体高大、童期长的问题,而且可以为以后山核桃的品种化奠定基础。Therefore, before the method of the present invention, the asexual reproduction technology of hickory nut still rests on test and trial stage, does not form a kind of mature method that can promote in a large number and repeated verification, therefore, hickory nut tree body is tall, and childhood period is long and The lack of growing branches of adult hickory trees suitable for scion is still a major problem that seriously restricts the development of hickory nut industry. Therefore, how to realize hickory root stock grafting and seedling cultivation is a key technology for developing the hickory industry. If a successful breakthrough can not only solve the problems of tall trees and long childhood, but also lay the foundation for future hickory varieties.
发明内容Contents of the invention
本发明目的是,针对上述山核桃树体高大、童期长和适宜作接穗的成年山核桃树生长枝缺少的技术问题,针对嫁接过程中,大批量接穗的培养、采集时间、保存条件以及嫁接时间的掌握等关键,提供一套完整的山核桃本砧嫁接苗的培育方法,以实现山核桃大批量嫁接苗的繁育,并达到树体矮化、提早结果的目的。The object of the present invention is, aiming at the technical problem that the above-mentioned hickory tree body is tall, the childhood period is long and the growing branch of the adult hickory tree suitable for scion is lacked, in the grafting process, the cultivation of large quantities of scion, collection time, storage conditions and grafting Time control and other key points are provided to provide a complete set of methods for cultivating hickory seedlings grafted on the stock, so as to realize the breeding of hickory grafted seedlings in large quantities, and to achieve the purpose of tree dwarfing and early fruiting.
为了达到上述的目的,本发明采用了以下的技术方案:In order to achieve the above-mentioned purpose, the present invention has adopted following technical scheme:
一种山核桃本砧嫁接苗的培育方法,其步骤如下:A method for cultivating grafted seedlings of hickory root stock, its steps are as follows:
(1)山核桃本砧苗圃的建立、播种和育苗;(1) establishment, sowing and seedling raising of hickory nut stock nursery;
(2)采穗圃的建立,以化香、山核桃、美国山核桃或湖南山核桃为砧木并以山核桃成年树生长枝为接穗培育采穗母本树;(2) The establishment of the fringe-picking nursery is to use Huaxiang, hickory, American hickory or Hunan hickory as rootstock and to cultivate the female parent tree of fringe with the growth branch of hickory adult tree as the scion;
(3)选取留床或在嫁接前一年11月初移植的地径≥0.7cm、健壮的1~2年生山核桃播种苗为砧木,株行距不小于12×25cm;(3) Select the healthy 1-2-year-old hickory seedlings with a ground diameter of ≥0.7cm and transplanted at the beginning of November of the year before grafting as rootstocks, and the row spacing between plants is not less than 12×25cm;
(4)接穗的采集与储藏,于上年的11月10日至当年的1月31日之间,从采穗圃采穗母本树上采集健壮、芽饱满、节间短的1~2年生生长枝为接穗,采集后在阴暗处放置12~36小时,再用塑料薄膜密封包裹,置于4℃冷库储藏;(4) Collection and storage of scions. Between November 10 of the previous year and January 31 of the current year, 1 to 2 scions with strong, full buds and short internodes were collected from the female parent tree in the scion picking garden. The annual growth branches are scions, which are placed in a dark place for 12 to 36 hours after collection, then sealed and wrapped with plastic film, and stored in a cold storage at 4°C;
(5)嫁接,当苗圃或移植地内30%砧木苗开始萌芽展叶时,于2~3周内完成嫁接;(5) grafting, when 30% rootstock seedlings in the nursery or the transplanting ground begin to germinate and spread leaves, complete the grafting within 2 to 3 weeks;
(6)嫁接苗护理,直至出圃。(6) Nursing of grafted seedlings until they emerge from the nursery.
不同的海拔的气候环境,砧穗状态都有所不同,现行的大部分山核桃育苗基地建立在海拔100米左右处,其适宜的嫁接时间在4月15日到4月28日;此时山核桃的韧皮部和木质部已开始容易分离。Depending on the climate environment at different altitudes, the state of the rootstock and ear is different. Most of the current pecan seedling bases are established at an altitude of about 100 meters, and the suitable grafting time is from April 15th to April 28th; The phloem and xylem of the walnut have begun to separate easily.
本发明的有益效果是:The beneficial effects of the present invention are:
一、利用根系发达、萌枝能力强的化香作为砧木,以成年山核桃树的生长枝为接穗,嫁接育成的幼树所建立的采穗圃,通过2年培育后,即可每年从这些母本树上大量采集到健壮、芽饱满、节间短的一年生枝优质接穗;同时,利用山核桃、美国山核桃或湖南山核桃为砧木,以山核桃成年树生长枝为接穗,嫁接育成的幼树建立采穗圃,这样在化香砧采穗母本树因为不亲和而开始死亡时,这些母本树经过几年的培育就可以开始大量采集一年生枝优质接穗了。One. Utilize Huaxiang with well-developed root system and strong budding ability as rootstock, and use the growth branch of adult hickory tree as scion, and the scion nursery established by the saplings that are grafted and bred, after 2 years of cultivation, can grow from these trees every year. A large number of high-quality scions of annual branches with strong, full buds and short internodes were collected from the female parent tree; at the same time, using hickory, American hickory or Hunan hickory as rootstocks, and using hickory adult tree growth branches as scions, grafted bred The saplings set up the ear-picking nursery, so that when the female parent trees start to die because of incompatibility, these female parent trees can start to collect a large number of high-quality scions of annual branches after several years of cultivation.
二、影响山核桃本砧嫁接成活率的因素,除山核桃形成层较薄、髓心大和单宁含量高外,经试验明确其关键还在于如何保障接穗的生活力,以促成愈伤组织形成及树体的后续生长。本发明选择了合适的接穗采集时间、保存条件以及嫁接时间,使接穗可以保有最佳的生活力,从而取得好的嫁接效果。2. Factors affecting the survival rate of hickory root stock grafting, in addition to the thin hickory nut cambium, large pith and high tannin content, the key is how to ensure the viability of the scion to promote callus formation and subsequent growth of the tree. The present invention selects the appropriate scion collection time, storage conditions and grafting time, so that the scion can maintain the best vitality, thereby achieving a good grafting effect.
三、采用了以上的技术方案,可将每年大量获得的,生活力旺盛的,包含有成年树枝生理成熟特点的接穗,适时嫁接到亲和力强的本砧苗木上,既提高了山核桃本砧嫁接成活率,大批量嫁接成活率可达90%左右,保存率80%以上;又使山核桃树体实现矮化、缩短童期、提早结果,一般高度可自行控制在5米以内,并在嫁接后的第4年结果,提早结果6-8年;同时也为山核桃树的批量化、品种化育苗,提供了可操作性强的山核桃本砧嫁接苗的培育方法,从而大大提高了山核桃的经济价值。Three, adopted the above technical scheme, can obtain in large quantities every year, the scion that vigor is exuberant, comprises the physiological maturity characteristic of adult tree branch, timely grafts on the native stock seedling with strong affinity, has not only improved hickory native stock grafting Survival rate, the survival rate of large-scale grafting can reach about 90%, and the preservation rate is more than 80%. It also makes the hickory tree dwarf, shortens the childhood period, and bears early. The general height can be controlled within 5 meters by itself, and the grafting The results in the last 4 years will result in 6-8 years earlier; meanwhile, it also provides a highly operable method for cultivating grafted seedlings of hickory root stocks for the batch and variety seedling cultivation of hickory trees, thereby greatly improving the quality of hickory trees. Economic value of walnuts.
具体实施方式Detailed ways
下面结合附图对本发明的具体实施方式做一个详细的说明。The specific implementation manner of the present invention will be described in detail below in conjunction with the accompanying drawings.
实施例1:Example 1:
一种山核桃本砧嫁接苗的培育方法,步骤如下:A method for cultivating grafted seedlings of hickory root stock, the steps are as follows:
(1)山核桃本砧苗圃的建立、播种和育苗;(1) establishment, sowing and seedling raising of hickory nut stock nursery;
(2)采穗圃的建立,以化香、山核桃、美国山核桃或湖南山核桃为砧木并以山核桃成年树生长枝为接穗嫁接培育采穗母本树;(2) The establishment of the fringe-picking nursery, take Huaxiang, hickory, American hickory or Hunan hickory as rootstock and take the growth branch of hickory adult tree as the scion grafting to cultivate the female parent tree of fringing;
(3)选取留床或在嫁接前一年11月初移植的地径≥0.7cm、健壮的1~2年生山核桃播种苗为砧木,株行距不小于12×25cm;(3) Select the healthy 1-2-year-old hickory seedlings with a ground diameter of ≥0.7cm and transplanted at the beginning of November of the year before grafting as rootstocks, and the row spacing between plants is not less than 12×25cm;
(4)接穗的采集与储藏,于上年的11月10日至当年的1月31日之间,从采穗圃采穗母本树上采集健壮、芽饱满、节间短的1~2年生生长枝为接穗,采集后在阴暗处放置12~36小时,再用塑料薄膜密封包裹,置于4℃冷库储藏;(4) Collection and storage of scions. Between November 10 of the previous year and January 31 of the current year, 1 to 2 scions with strong, full buds and short internodes were collected from the female parent tree in the scion picking garden. The annual growth branches are scions, which are placed in a dark place for 12 to 36 hours after collection, then sealed and wrapped with plastic film, and stored in a cold storage at 4°C;
(5)嫁接,当苗圃或移植地内30%砧木苗开始萌芽展叶时,于2~3周内完成嫁接;(5) grafting, when 30% rootstock seedlings in the nursery or the transplanting ground begin to germinate and spread leaves, complete the grafting within 2 to 3 weeks;
(6)嫁接苗护理,直至出圃。(6) Nursing of grafted seedlings until they emerge from the nursery.
我们在浙江临安市藻溪镇有100亩山核桃苗木繁育基地(海拔100米左右),建立有过渡时期的采穗圃,利用根系发达、萌枝能力强的化香作为砧木嫁接山核桃大树上的生长枝形成母本树,经两年培育后即可大量采集到健壮、芽饱满、节间短的一年生枝优质接穗;同时,利用山核桃(也可以为美国山核桃或湖南山核桃)为砧木,以山核桃成年树生长枝为接穗,嫁接育成的幼树建立采穗圃,经过几年的培育,这些母本树就可以在化香砧采穗母本树因为不亲和而开始死亡时,开始大量采集一年生枝优质接穗,从而可以每年提供大量优质接穗以保证大量培育山核桃本砧嫁接苗。We have a 100-acre hickory seedling breeding base (about 100 meters above sea level) in Zaoxi Town, Lin'an City, Zhejiang Province. We have established a transitional ear picking nursery, and used Huaxiang, which has a well-developed root system and strong sprouting ability, as a rootstock to graft large hickory trees. The growth branches on the top form the female parent tree, and after two years of cultivation, a large number of high-quality scions of annual branches with strong, full buds and short internodes can be collected; at the same time, hickory (also can be American hickory or Hunan hickory) As the rootstock, the growing branch of the adult hickory tree is used as the scion, and the saplings bred by grafting are used to establish the ear picking nursery. After several years of cultivation, these female trees can be picked on the Huaxiang stock When dying, start to collect the high-quality scion of annual branch in a large number, thereby can provide a large amount of high-quality scion every year to guarantee to cultivate the grafted seedling of hickory nut main stock in a large amount.
在2001年3月20日左右,选取地径≥0.7cm、健壮的一年生山核桃苗为砧木,集中移植,株行距为12×25cm。到了4月中下旬,选择叶子刚刚开始舒展的苗木植株,距地面约10cm处截干,从横截面约1/4~1/5处纵切,深约3cm,在小切面木质部约2.5cm处,以约35度斜角向内斜切一刀,剔除上部木质部,保留韧皮部;取单芽接穗,长约4.5cm:芽上1.5cm,芽下约3.0cm,在其芽背面切成长切面约3cm,芽下约0.5cm处下刀切成浅切面(切去韧皮部或略带木质部),至2.5cm处转约35度角向内斜切至底部,呈锲形;接穗长切面形成层对准砧木大切面的两边形成层,至少对准一边,浅切面与砧木小切面韧皮部和末端形成层贴合,然后,用尼龙薄膜密封全部切面,再绑紧,绑扎时避免砧穗形成层移位,露出接穗芽头。Around March 20, 2001, the robust annual hickory seedlings with a ground diameter of ≥0.7 cm were selected as rootstocks, and transplanted intensively, with a row spacing of 12×25 cm. In mid-to-late April, select seedling plants whose leaves have just begun to stretch, cut off at about 10cm from the ground, cut longitudinally from about 1/4 to 1/5 of the cross section, and cut about 3cm deep, at about 2.5cm in the xylem of the small cut surface , cut inward at an oblique angle of about 35 degrees, remove the upper xylem, and retain the phloem; take a single-bud scion, about 4.5cm long: 1.5cm above the bud, about 3.0cm below the bud, and cut the long section on the back of the bud about 3cm , cutting at about 0.5cm below the bud and cutting into a shallow section (cutting off the phloem or a little xylem), turning an angle of about 35 degrees at 2.5cm and cutting obliquely inward to the bottom, forming a wedge shape; the long section of the scion is aligned with the cambium The cambium on both sides of the large cut surface of the rootstock should be aligned with at least one side, and the shallow cut surface should be attached to the phloem and terminal cambium of the small cut surface of the rootstock. Then, seal the entire cut surface with a nylon film, and then tie it tightly. Expose the scion bud head.
嫁接完成之后的第20天开始抹去山核桃砧木上面长出来的萌芽条,每隔25天查看一次,到了7月20日基本不需要大量的抹芽。到当年的11月份统计成活率达到91%。2002年上山造林,成活率达90%,2005年调查60%苗木已经开花结果。苗木接口表现正常,树体在4米以内,在提早结果的同时实现了山核桃的矮化的安全管理模式。On the 20th day after the grafting is completed, start to wipe off the bud strips growing on the hickory rootstock, and check it every 25 days. By July 20, there is basically no need for a lot of bud wiping. By November of that year, the statistical survival rate reached 91%. In 2002, afforestation was carried out on the mountain, and the survival rate reached 90%. In 2005, 60% of the seedlings had blossomed and bore fruit. The interface of the seedlings is normal, and the tree body is within 4 meters, and the dwarfing safety management mode of hickory has been realized while the fruit is early.
因为山核桃的播种和其他防病虫害等后续管理均为现有技术,恕不赘述。Because the follow-up management such as sowing of hickory nuts and other diseases and insect pests are prior art, will not repeat them.
上述的嫁接的具体操作方法(即嫁接方法)为现有技术,可以是上面的切接方法也可以采用同样适用的贴接方法。即,在砧木距地面约20cm处截干,在其下约10cm处斜切,末端深入木质部约0.3cm,切面长约3cm;在小片木质部约2.5cm处贴接,它可避免砧木切面枯顶,增大愈合面;以约35度角向内斜切至底部,剔除小片木质部;其余操作同上。The above-mentioned specific operation method of grafting (that is, the grafting method) is the prior art, and it can be the above cutting method or the same applicable sticking method. That is, the rootstock is cut off at about 20cm from the ground, cut obliquely at about 10cm below it, the end penetrates into the xylem about 0.3cm, and the length of the cut surface is about 3cm; sticking at a small piece of xylem about 2.5cm can prevent the cut surface of the rootstock from withering , to increase the healing surface; obliquely cut inward at an angle of about 35 degrees to the bottom, and remove small pieces of xylem; the rest of the operation is the same as above.
本发明不局限于以上具体实施方式,只要是采用了本发明方法提供的接穗的培养方法采集时间、保存条件以及嫁接时间,无论是采用何种嫁接方法,进行何种等同变换和修饰,都将落入本发明的保护范围之内。The present invention is not limited to above-mentioned specific embodiment, as long as adopt the culture method collection time, preservation condition and grafting time of the scion provided by the method of the present invention, no matter be to adopt which kind of grafting method, carry out what kind of equivalent conversion and modification, all will Fall within the protection scope of the present invention.
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