CN100497642C - 修饰真核细胞的方法 - Google Patents

修饰真核细胞的方法 Download PDF

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Publication number
CN100497642C
CN100497642C CNB018216609A CN01821660A CN100497642C CN 100497642 C CN100497642 C CN 100497642C CN B018216609 A CNB018216609 A CN B018216609A CN 01821660 A CN01821660 A CN 01821660A CN 100497642 C CN100497642 C CN 100497642C
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gene
cells
embryonic stem
chromosomal locus
ltvec
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CN1484707A (zh
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A·N·埃科诺米德斯
A·J·默菲
D·M·瓦伦泽拉
D·弗伦德维
G·D·彦科普洛斯
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Regeneron Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB018216609A 2000-10-31 2001-10-31 修饰真核细胞的方法 Expired - Lifetime CN100497642C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US24466500P 2000-10-31 2000-10-31
US60/244,665 2000-10-31
US09/732,234 US6586251B2 (en) 2000-10-31 2000-12-07 Methods of modifying eukaryotic cells
US09/732,234 2000-12-07

Publications (2)

Publication Number Publication Date
CN1484707A CN1484707A (zh) 2004-03-24
CN100497642C true CN100497642C (zh) 2009-06-10

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US (1) US6586251B2 (https=)
EP (1) EP1399575B1 (https=)
JP (7) JP5339566B2 (https=)
KR (1) KR100833136B1 (https=)
CN (1) CN100497642C (https=)
AT (1) ATE332388T1 (https=)
AU (2) AU2004802A (https=)
BR (1) BRPI0115096B1 (https=)
CA (1) CA2426398C (https=)
CZ (1) CZ304856B6 (https=)
DE (1) DE60121372T2 (https=)
HU (1) HU227639B1 (https=)
MX (1) MXPA03003797A (https=)
NO (1) NO332300B1 (https=)
NZ (1) NZ525511A (https=)
PL (1) PL204759B1 (https=)
RU (1) RU2290441C2 (https=)
WO (1) WO2002036789A2 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105010238A (zh) * 2010-02-08 2015-11-04 瑞泽恩制药公司 共有轻链小鼠
CN107002093A (zh) * 2014-06-26 2017-08-01 瑞泽恩制药公司 用于靶向遗传修饰的方法和组合物，以及这些组合物的使用方法

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