CN100343388C - 热稳定的核糖核酸酶h - Google Patents

热稳定的核糖核酸酶h Download PDF

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Publication number
CN100343388C
CN100343388C CNB038205440A CN03820544A CN100343388C CN 100343388 C CN100343388 C CN 100343388C CN B038205440 A CNB038205440 A CN B038205440A CN 03820544 A CN03820544 A CN 03820544A CN 100343388 C CN100343388 C CN 100343388C
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CN
China
Prior art keywords
rnase
polypeptide
nucleic acid
activity
seq
Prior art date
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Expired - Fee Related
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CNB038205440A
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English (en)
Chinese (zh)
Other versions
CN1678736A (zh
Inventor
外园成和
上森隆司
田中哲基
加藤郁之进
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Takara Bio Inc
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Takara Bio Inc
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Publication of CN1678736A publication Critical patent/CN1678736A/zh
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Publication of CN100343388C publication Critical patent/CN100343388C/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Soft Magnetic Materials (AREA)
CNB038205440A 2002-08-30 2003-08-26 热稳定的核糖核酸酶h Expired - Fee Related CN100343388C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP254153/2002 2002-08-30
JP2002254153 2002-08-30

Publications (2)

Publication Number Publication Date
CN1678736A CN1678736A (zh) 2005-10-05
CN100343388C true CN100343388C (zh) 2007-10-17

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ID=31972825

Family Applications (1)

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CNB038205440A Expired - Fee Related CN100343388C (zh) 2002-08-30 2003-08-26 热稳定的核糖核酸酶h

Country Status (10)

Country Link
US (2) US7572617B2 (https=)
EP (1) EP1553172B1 (https=)
JP (1) JP4105693B2 (https=)
KR (1) KR20050058485A (https=)
CN (1) CN100343388C (https=)
AT (1) ATE432980T1 (https=)
AU (1) AU2003257699A1 (https=)
DE (1) DE60327875D1 (https=)
TW (1) TW200418984A (https=)
WO (1) WO2004020621A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7888107B2 (en) 2006-07-24 2011-02-15 Nanosphere, Inc. System using self-contained processing module for detecting nucleic acids
US7625746B2 (en) 2006-07-24 2009-12-01 Nanosphere, Inc. Method of denaturing and fragmenting DNA or RNA using ultrasound
EP2644708B1 (en) * 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. RNASE-H-based assays utilizing modified RNA monomers
EP2333109B1 (en) * 2008-09-03 2013-08-07 Takara Bio, Inc. Composition for detection of rna
EP4324919A3 (en) 2015-10-14 2024-05-29 Novozymes A/S Polypeptide variants

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002022831A1 (en) * 2000-09-14 2002-03-21 Takara Bio Inc. Thermotolerant ribonuclease h

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2533671B2 (ja) 1990-04-26 1996-09-11 株式会社蛋白工学研究所 組換えｄｎａ技術による好熱菌リボヌクレア―ゼｈの製造法
US5268289A (en) 1991-12-27 1993-12-07 Epicentre Technologies Corp. Thermostable ribonuclease H
US5610066A (en) 1993-12-10 1997-03-11 Amersham Life Science, Inc. Nucleic acid modifying proteins from Pyrococcus furiosus
JPH1132772A (ja) 1997-07-24 1999-02-09 Mitsubishi Chem Corp 耐熱性リボヌクレアーゼｈ及びそれをコードするｄｎａ
ATE362532T1 (de) 1999-03-19 2007-06-15 Takara Bio Inc Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers
EA005739B1 (ru) 2000-08-23 2005-06-30 Такара Био Инк. Способ амплификации нуклеиновых кислот

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002022831A1 (en) * 2000-09-14 2002-03-21 Takara Bio Inc. Thermotolerant ribonuclease h

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PLASMID PGS5 FROM THE HYPERTHERMOPHILICARCHAEON ARCHAEOGLOBUS PROFUNDUS ISNEGATIVELY SUPERCOILED LOPEZ GARCIA P.等,J.BACTERIOL,Vol.182 No.17 2000 *

Also Published As

Publication number Publication date
WO2004020621A1 (ja) 2004-03-11
DE60327875D1 (de) 2009-07-16
US7572617B2 (en) 2009-08-11
TW200418984A (en) 2004-10-01
CN1678736A (zh) 2005-10-05
JP4105693B2 (ja) 2008-06-25
WO2004020621A8 (ja) 2005-03-10
EP1553172A4 (en) 2006-03-15
KR20050058485A (ko) 2005-06-16
EP1553172A1 (en) 2005-07-13
US20090269809A1 (en) 2009-10-29
JPWO2004020621A1 (ja) 2005-12-15
EP1553172B1 (en) 2009-06-03
ATE432980T1 (de) 2009-06-15
US20070037247A1 (en) 2007-02-15
TWI311153B (https=) 2009-06-21
AU2003257699A8 (en) 2004-03-19
AU2003257699A1 (en) 2004-03-19
US7932071B2 (en) 2011-04-26

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Granted publication date: 20071017

Termination date: 20110826