CL2021000629A1 - Microorganismo para la producción de l-aminoácido con actividad mejorada de a-glucosidasa y procedimiento para la producción de l-aminoácido usando el mismo - Google Patents

Microorganismo para la producción de l-aminoácido con actividad mejorada de a-glucosidasa y procedimiento para la producción de l-aminoácido usando el mismo

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Publication number
CL2021000629A1
CL2021000629A1 CL2021000629A CL2021000629A CL2021000629A1 CL 2021000629 A1 CL2021000629 A1 CL 2021000629A1 CL 2021000629 A CL2021000629 A CL 2021000629A CL 2021000629 A CL2021000629 A CL 2021000629A CL 2021000629 A1 CL2021000629 A1 CL 2021000629A1
Authority
CL
Chile
Prior art keywords
amino acid
acid production
microorganism
glucosidase activity
improved
Prior art date
Application number
CL2021000629A
Other languages
English (en)
Inventor
Hyung Joon Kim
Kwang Woo Lee
Hyo Jeong Byun
Yong Uk Shin
Kyungrim Kim
Jung Kee Lee
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of CL2021000629A1 publication Critical patent/CL2021000629A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención describe un microorganismo para la producción de un L-aminoácido con actividad mejorada de a-glucosidasa y a un procedimiento para la producción de un L-aminoácido usando el mismo. El microorganismo del género Corynebacterium que produce un L-aminoácido tiene una actividad mejorada de a-glucosidasa, mejorando así el rendimiento de producción de L-aminoácido. Por lo tanto, el microorganismo puede usarse de manera muy útil para la producción de L-aminoácidos.
CL2021000629A 2018-09-28 2021-03-16 Microorganismo para la producción de l-aminoácido con actividad mejorada de a-glucosidasa y procedimiento para la producción de l-aminoácido usando el mismo CL2021000629A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020180116540A KR102112240B1 (ko) 2018-09-28 2018-09-28 알파-글루코시다제의 활성이 강화된 l-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산 생산 방법

Publications (1)

Publication Number Publication Date
CL2021000629A1 true CL2021000629A1 (es) 2021-09-24

Family

ID=69952917

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2021000629A CL2021000629A1 (es) 2018-09-28 2021-03-16 Microorganismo para la producción de l-aminoácido con actividad mejorada de a-glucosidasa y procedimiento para la producción de l-aminoácido usando el mismo

Country Status (15)

Country Link
US (1) US11293014B2 (es)
EP (1) EP3656863A4 (es)
JP (1) JP7286758B2 (es)
KR (1) KR102112240B1 (es)
CN (1) CN111247250B (es)
AU (1) AU2019349396B2 (es)
BR (1) BR112019019867B1 (es)
CL (1) CL2021000629A1 (es)
IL (1) IL281567A (es)
MX (1) MX2021002814A (es)
PE (1) PE20210686A1 (es)
PH (1) PH12021550347A1 (es)
RU (1) RU2732338C1 (es)
WO (1) WO2020067618A1 (es)
ZA (1) ZA202101456B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111944781A (zh) * 2020-09-03 2020-11-17 廊坊梅花生物技术开发有限公司 一种突变的高丝氨酸激酶及其应用
KR20220156323A (ko) * 2021-05-18 2022-11-25 씨제이제일제당 (주) Agl 단백질의 활성이 강화된, 아미노산을 생산하는 미생물 및 이를 이용한 아미노산 생산 방법

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0159812B1 (ko) 1995-12-20 1998-11-16 손경식 코리네박테리움 글루타미컴 씨에이치 77 및 이 균주를 이용한 l-라이신의 제조 방법
ES2218009T3 (es) * 1999-02-20 2004-11-16 Degussa Ag Procedimiento para la preparacion fermentativa de l-aminoacidos utilizando bacterias corineformes.
DE10261579A1 (de) 2002-12-23 2004-07-15 Basf Ag Verfahren zur Herstellung von Trehalose-freien Aminosäuren
JP2004261150A (ja) * 2003-03-04 2004-09-24 Ajinomoto Co Inc 目的物質の製造法
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
BRPI0716980A2 (pt) 2006-09-15 2013-10-22 Cj Cheiljedang Corp Corynebacteria com produtividade de l-lisina aumentada e método de produção da l-lisina usando a mesma
KR100838038B1 (ko) 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법
JP2011067095A (ja) 2008-01-10 2011-04-07 Ajinomoto Co Inc 発酵法による目的物質の製造法
WO2011100571A1 (en) * 2010-02-12 2011-08-18 Bp Corporation North America Inc. Bacteria capable of using cellobiose and methods of use thereof
DE102011007313A1 (de) * 2011-04-13 2012-10-18 Henkel Ag & Co. Kgaa Expressionsverfahren
US9200300B2 (en) * 2012-01-10 2015-12-01 Cj Cheiljedang Corporation Microorganisms of Corynebacterium which can utilize xylose and method for producing L-lysine using same
KR101484108B1 (ko) 2012-08-22 2015-01-19 국립대학법인 울산과학기술대학교 산학협력단 ａｓｃＢ 또는 ｃｈｂＦ 유전자의 과발현을 통해 셀로바이오스의 이용률이 증가되고 셀로바이오스 및 기타 당을 동시에 이용할 수 있는 변이미생물을 제조하는 방법
WO2015060391A1 (ja) 2013-10-23 2015-04-30 味の素株式会社 目的物質の製造法
JP6302073B2 (ja) 2014-08-21 2018-03-28 公益財団法人地球環境産業技術研究機構 コリネ型細菌形質転換体、及びそれを用いる有機化合物の製造方法
PL3508580T3 (pl) 2016-08-31 2022-04-25 Cj Cheiljedang Corporation Nowy promotor i jego zastosowanie
EP3415622A1 (en) 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases

Also Published As

Publication number Publication date
CN111247250B (zh) 2022-07-05
JP7286758B2 (ja) 2023-06-05
IL281567A (en) 2021-05-31
BR112019019867B1 (pt) 2022-05-10
KR102112240B1 (ko) 2020-05-18
CN111247250A (zh) 2020-06-05
EP3656863A1 (en) 2020-05-27
EP3656863A4 (en) 2021-04-14
BR112019019867A2 (pt) 2021-04-13
KR20200036647A (ko) 2020-04-07
US20210017510A1 (en) 2021-01-21
JP2021534765A (ja) 2021-12-16
WO2020067618A1 (ko) 2020-04-02
AU2019349396A1 (en) 2021-04-15
WO2020067618A8 (ko) 2020-09-24
MX2021002814A (es) 2021-05-12
RU2732338C1 (ru) 2020-09-15
US11293014B2 (en) 2022-04-05
PE20210686A1 (es) 2021-04-08
EP3656863A9 (en) 2020-11-11
ZA202101456B (en) 2022-06-29
PH12021550347A1 (en) 2021-10-04
AU2019349396B2 (en) 2023-04-13

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