CA3138752C - Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing - Google Patents

Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing Download PDF

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Publication number
CA3138752C
CA3138752C CA3138752A CA3138752A CA3138752C CA 3138752 C CA3138752 C CA 3138752C CA 3138752 A CA3138752 A CA 3138752A CA 3138752 A CA3138752 A CA 3138752A CA 3138752 C CA3138752 C CA 3138752C
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Prior art keywords
flow cell
reservoirs
reservoir
valve
fluidic
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CA3138752A
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English (en)
French (fr)
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CA3138752A1 (en
Inventor
Dale Buermann
John A. Moon
Bryan Crane
Mark Wang
Stanley S. Hong
Jason Harris
Matthew Hage
Mark J. Nibbe
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Illumina Inc
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Illumina Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G01N2021/0307—Insert part in cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
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    • G01N2021/058—Flat flow cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
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    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
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  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
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  • Optics & Photonics (AREA)
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  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Saccharide Compounds (AREA)
  • Catalysts (AREA)
CA3138752A 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing Active CA3138752C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261619784P 2012-04-03 2012-04-03
US61/619,784 2012-04-03
CA2867665A CA2867665C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2867665A Division CA2867665C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Publications (2)

Publication Number Publication Date
CA3138752A1 CA3138752A1 (en) 2013-10-10
CA3138752C true CA3138752C (en) 2024-02-06

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CA3138752A Active CA3138752C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
CA2867665A Active CA2867665C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Family Applications After (1)

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CA2867665A Active CA2867665C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Country Status (13)

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US (5) US9193996B2 (enExample)
EP (2) EP4219012A1 (enExample)
JP (1) JP6159391B2 (enExample)
KR (3) KR102118211B1 (enExample)
CN (1) CN204832037U (enExample)
AU (4) AU2013243998B2 (enExample)
BR (2) BR112014024789B1 (enExample)
CA (2) CA3138752C (enExample)
ES (1) ES2949570T3 (enExample)
IL (3) IL234864B (enExample)
IN (1) IN2014DN07992A (enExample)
MX (1) MX337140B (enExample)
WO (1) WO2013151622A1 (enExample)

Families Citing this family (333)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
HRP20151294T1 (hr) 2010-04-05 2016-03-11 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
IN2014DN07992A (enExample) 2012-04-03 2015-05-01 Illumina Inc
US8932815B2 (en) 2012-04-16 2015-01-13 Biological Dynamics, Inc. Nucleic acid sample preparation
US8603791B2 (en) 2012-04-16 2013-12-10 Biological Dynamics, Inc. Nucleic acid sample preparation
EP3511423B2 (en) 2012-10-17 2024-05-29 Spatial Transcriptomics AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
LT3013984T (lt) 2013-06-25 2023-06-12 Prognosys Biosciences, Inc. Biologinių taikinių erdvinių modelių mėginyje nustatymo būdai
SI3030645T1 (sl) * 2013-08-08 2023-03-31 Illumina, Inc. Fluidni sistem za dovajanje reagenta v pretočno celico
EP3039409B1 (en) 2013-08-28 2017-11-08 Illumina, Inc. Optical alignment tool
US10540783B2 (en) * 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
PL3077943T3 (pl) 2013-12-03 2020-11-30 Illumina, Inc. Sposoby i układy do analizy danych obrazowych
JP7011392B2 (ja) 2014-02-18 2022-01-26 イラミーナ インコーポレーテッド Ｄｎａプロファイリングのための方法および組成物
US10767219B2 (en) * 2014-03-11 2020-09-08 Illumina, Inc. Disposable, integrated microfluidic cartridge and methods of making and using same
CA2951118C (en) 2014-05-16 2024-01-09 Illumina, Inc. Nucleic acid synthesis techniques
EP3148697A1 (en) * 2014-05-27 2017-04-05 Illumina, Inc. Systems and methods for biochemical analysis including a base instrument and a removable cartridge
DK3669985T3 (da) 2014-06-05 2022-05-09 Illumina Inc Systemer inklusive en rotationsventil til mindst en af prøveforberedelse eller prøveanalyse
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
GB201416422D0 (en) * 2014-09-17 2014-10-29 Illumina Cambridge Ltd Flexible tape-based chemistry apparatus
SG11201701974XA (en) 2014-10-09 2017-04-27 Illumina Inc Method and device for separating immiscible liquids to effectively isolate at least one of the liquids
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
EP3215616B1 (en) 2014-11-05 2019-12-18 Illumina Cambridge Limited Reducing dna damage during sample preparation and sequencing using siderophore chelators
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
SG11201703693UA (en) 2014-11-11 2017-06-29 Illumina Cambridge Ltd Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
WO2016130962A1 (en) 2015-02-13 2016-08-18 Abbott Laboratories Automated storage modules for diagnostic analyzer liquids and related systems and methods
EP3271073B1 (en) 2015-03-20 2019-06-12 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
USD766761S1 (en) * 2015-03-20 2016-09-20 Life Technologies Corporation Analytical equipment
JP6567685B2 (ja) 2015-03-24 2019-08-28 イラミーナ インコーポレーテッド 生物学的又は化学的な解析用のサンプルを撮像する方法、キャリヤ組立体、及びシステム
US20180100192A1 (en) 2015-03-31 2018-04-12 lllumina Cambridge Limited Surface concatemerization of templates
US11971354B2 (en) 2015-04-08 2024-04-30 Molecular Devices, Llc Methods and systems for fluorescence detection using infrared dyes
US10379046B2 (en) * 2015-04-08 2019-08-13 Molecular Devices, Llc Method and system for multiplexed time-resolved fluorescence detection
CN120425026A (zh) 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
US9222935B1 (en) * 2015-05-28 2015-12-29 Pixcell Medical Technologies Ltd Fluid sample analysis system
US10640809B2 (en) 2015-05-29 2020-05-05 Epicentre Technologies Corporation Methods of analyzing nucleic acids
US10590464B2 (en) 2015-05-29 2020-03-17 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
EP3320111B1 (en) 2015-07-06 2021-05-05 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
CA3242290A1 (en) 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
EP3334839B1 (en) 2015-08-14 2021-03-03 Illumina, Inc. Systems and methods using magnetically-responsive sensors for determining a genetic characteristic
EP3338096B1 (en) 2015-08-24 2024-03-06 Illumina, Inc. In-line pressure accumulator and flow-control system for biological or chemical assays
JP6720526B2 (ja) * 2015-12-25 2020-07-08 東洋紡株式会社 検査装置
DE202017100081U1 (de) * 2016-01-11 2017-03-19 Illumina, Inc. Detektionsvorrichtung mit einem Mikrofluorometer, einem fluidischen System und einem Durchflusszellen-Rastklemmenmodul
USD819829S1 (en) * 2016-02-12 2018-06-05 Illumina, Inc. Sequencing cartridge
US10508305B2 (en) * 2016-02-28 2019-12-17 Damoun Nashtaali DNA sequencing and processing
CA3018900A1 (en) * 2016-03-24 2017-09-28 Biological Dynamics, Inc. Disposable fluidic cartridge and components
WO2017172798A1 (en) 2016-03-28 2017-10-05 Illumina, Inc. Multi-plane microarrays
ITUA20162851A1 (it) * 2016-04-04 2017-10-04 Euritmi S A S Di Dalla Sega Daniela E C Sistema per microscopia
US11535883B2 (en) 2016-07-22 2022-12-27 Illumina, Inc. Single cell whole genome libraries and combinatorial indexing methods of making thereof
WO2018025041A1 (en) 2016-08-05 2018-02-08 Vital Signs Solutions Limited Device and method for liquid analysis to detect biomarkers
AU2017331281A1 (en) * 2016-09-23 2019-05-23 ArcherDX, Inc. Operation of a library preparation system to perform a protocol on a biological sample
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
USD825078S1 (en) * 2016-10-14 2018-08-07 Illumina, Inc. Sequencing cartridge
TWI781669B (zh) 2016-10-14 2022-10-21 美商伊路米納有限公司 卡匣總成
CN108265112B (zh) 2016-12-30 2023-04-25 深圳市真迈生物科技有限公司 对序列测定反应进行控制的方法、序列测定系统和控制装置
GB201704761D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Common line selector valve for a system
GB201704768D0 (en) 2017-01-05 2017-05-10 Illumina Inc Flow cell liquid degassing systema and method
GB201704760D0 (en) 2017-01-05 2017-05-10 Illumina Inc Reagent nozzle sipper mixing system and method
GB201704763D0 (enExample) 2017-01-05 2017-05-10 Illumina Inc
GB201704766D0 (en) 2017-01-05 2017-05-10 Illumia Inc System and methods for selective effluent collection
GB201704772D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Automated volumetric reagent delivery testing
GB201704747D0 (en) 2017-01-05 2017-05-10 Illumina Inc Reagent mixing system and methods
KR102629171B1 (ko) 2017-01-06 2024-01-25 일루미나, 인코포레이티드 페이징 보정
TWI689720B (zh) * 2017-01-07 2020-04-01 美商伊路米納有限公司 固態檢驗設備及使用方法
AU2018210316A1 (en) 2017-01-17 2019-06-27 Illumina, Inc. Oncogenic splice variant determination
SG11201906442TA (en) 2017-02-01 2019-08-27 Illumina Inc System and method with fiducials responding to multiple excitation frequencies
GB201701691D0 (en) 2017-02-01 2017-03-15 Illumina Inc System and method with reflective fiducials
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
GB201701688D0 (en) * 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
KR102246285B1 (ko) * 2017-03-07 2021-04-29 일루미나, 인코포레이티드 단일 광원, 2-광학 채널 서열분석
WO2018175424A1 (en) * 2017-03-22 2018-09-27 The Board Of Trustees Of The University Of Illinois System for rapid, portable, and multiplexed detection and identification of pathogen specific nucleic acid sequences
CN117116360A (zh) 2017-03-30 2023-11-24 Illumina公司 基因组数据分析系统和方法
EP3607087A4 (en) 2017-04-04 2020-12-30 Omniome, Inc. FLUIDIC APPARATUS AND USEFUL METHODS FOR CHEMICAL AND BIOLOGICAL REACTIONS
CN110785492B (zh) 2017-04-23 2024-04-30 伊鲁米纳剑桥有限公司 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
CA3062585A1 (en) 2017-05-08 2018-11-15 Biological Dynamics, Inc. Methods and systems for analyte information processing
FI3981884T3 (fi) 2017-06-07 2023-09-21 Univ Oregon Health & Science Yksittäissolujen kokogenomikirjastoja metylaatiosekvensointia varten
WO2018236631A1 (en) 2017-06-20 2018-12-27 Illumina, Inc. METHODS AND COMPOSITIONS FOR RESOLVING INEFFICIENCY IN AMPLIFICATION REACTIONS
US20200202977A1 (en) 2017-07-31 2020-06-25 Illumina, Inc. Sequencing system with multiplexed biological sample aggregation
EP3662083B1 (en) 2017-08-01 2024-08-28 Illumina, Inc. Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
AU2018317826B2 (en) 2017-08-15 2022-11-24 Pacific Biosciences Of California, Inc. Scanning apparatus and methods useful for detection of chemical and biological analytes
CN111566261B (zh) 2017-08-18 2025-03-25 诺迪勒思附属公司 选择结合试剂的方法
CA3086538A1 (en) 2017-12-15 2019-06-20 Evanostics Llc Optical reader for analyte testing
US11691143B2 (en) 2017-12-15 2023-07-04 Creoptix Ag Assemblies and methods for screening sample fluids
WO2019126388A1 (en) 2017-12-19 2019-06-27 Biological Dynamics, Inc. Methods and devices for detection of multiple analytes from a biological sample
KR20250152680A (ko) 2017-12-29 2025-10-23 노틸러스 서브시디어리, 인크. 단백질 식별을 위한 디코딩 접근법
US11883833B2 (en) 2018-04-02 2024-01-30 Biological Dynamics, Inc. Dielectric materials
WO2019195225A1 (en) 2018-04-02 2019-10-10 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
US11441978B1 (en) * 2018-04-12 2022-09-13 Elemental Scientific, Inc. Automatic evaporative sample preparation
WO2019203986A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
WO2019221913A1 (en) 2018-05-15 2019-11-21 Illumina, Inc. Flow cell with flexible connection
NL2021147B1 (en) * 2018-05-15 2019-11-21 Illumina Inc Flow cell with flexible connection
IL271454B2 (en) 2018-05-17 2025-04-01 Illumina Inc Rapid single-cell genetic sequencing with low Aggregation bias
WO2019236599A2 (en) 2018-06-04 2019-12-12 Illumina, Inc. High-throughput single-cell transcriptome libraries and methods of making and of using
JP6688342B2 (ja) 2018-07-06 2020-04-28 日本板硝子株式会社 反応処理装置
DE102018212489A1 (de) * 2018-07-26 2020-01-30 Implen GmbH Vorrichtung für eine lichtspektroskopische Analyse
EP3844308A1 (en) 2018-08-28 2021-07-07 10X Genomics, Inc. Resolving spatial arrays
CN120775968A (zh) 2018-08-28 2025-10-14 10X基因组学股份有限公司 用于生物样品中转座酶介导的空间标记和分析基因组dna的方法
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
NL2021970B1 (en) * 2018-10-05 2020-05-12 Illumina Inc System and method for sequestered wash buffered reuse
US11426723B2 (en) * 2018-10-05 2022-08-30 Illumina, Inc. System and method for sequestered wash buffered reuse
CN113167783A (zh) * 2018-10-18 2021-07-23 加利福尼亚大学董事会 用于流体样本中运动物体的基于运动的无标记检测的设备和方法
US10768173B1 (en) * 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
EP3884048A4 (en) 2018-11-20 2022-08-17 Nautilus Biotechnology, Inc. DESIGN AND SELECTION OF AFFINITY REAGENTS
CN111277744B (zh) * 2018-12-04 2022-04-15 武汉华大智造科技有限公司 对焦方法及系统、基因测序仪、计算机可读存储介质
EP3891304A1 (en) 2018-12-04 2021-10-13 Omniome, Inc. Mixed-phase fluids for nucleic acid sequencing and other analytical assays
CN113767176A (zh) 2018-12-10 2021-12-07 10X基因组学有限公司 成像系统硬件
US11879840B2 (en) * 2018-12-11 2024-01-23 Carrier Corporation Calibration of an optical detector using a micro-flow chamber
EP3894598B1 (en) * 2018-12-14 2024-01-03 Illumina Cambridge Limited Decreasing phasing with unlabeled nucleotides during sequencing
US12066383B2 (en) 2018-12-18 2024-08-20 Aspida Dx Inc. Optical analyte detection
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11493441B2 (en) 2019-01-15 2022-11-08 Wyatt Technology Corporation Flow cell, read head, and skid attachment
WO2020167574A1 (en) 2019-02-14 2020-08-20 Omniome, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN113613787B (zh) 2019-02-20 2023-06-13 加利福尼亚太平洋生物科学股份有限公司 用于检测化学和生物分析物的扫描装置和方法
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
CA3113841A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
EP3938538A1 (en) 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
WO2020191387A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Artificial intelligence-based base calling
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
EP3887542A1 (en) 2019-03-22 2021-10-06 10X Genomics, Inc. Three-dimensional spatial analysis
CA3135206A1 (en) 2019-04-29 2020-11-05 Pierre Indermuhle Methods and systems for integrated on-chip single-molecule detection
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
US10656368B1 (en) * 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
EP4265628B1 (en) 2019-09-10 2025-11-05 Pacific Biosciences of California, Inc. Reversible modification of nucleotides
KR20220063116A (ko) * 2019-09-18 2022-05-17 일루미나, 인코포레이티드 시스템 및 관련 샘플 로딩 매니폴드 조립체
MX2021006292A (es) * 2019-09-18 2021-08-24 Illumina Inc Sistemas y unidades múltiples de bomba relacionadas.
EP4036554B1 (en) * 2019-09-24 2025-09-17 MGI Tech Co., Ltd. Biochemical substance analysis system and device
CN117036248A (zh) 2019-10-01 2023-11-10 10X基因组学有限公司 用于识别组织样品中的形态学模式的系统和方法
CN114901946A (zh) * 2019-10-29 2022-08-12 宽腾矽公司 用于生物分析应用的流体的蠕动泵送以及相关的方法、系统和装置
US12011716B2 (en) 2019-10-29 2024-06-18 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
CN115004260A (zh) 2019-11-18 2022-09-02 10X基因组学有限公司 用于组织分类的系统和方法
CA3158888A1 (en) 2019-11-21 2021-05-27 Yifeng YIN Spatial analysis of analytes
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
AU2020407641A1 (en) 2019-12-19 2021-09-23 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
CN114981011B (zh) * 2019-12-30 2024-03-29 伊鲁米那有限公司 流通池组件和相关试剂选择器阀
MX2021016073A (es) * 2019-12-31 2022-03-25 Illumina Inc Funcionalidad de enfoque automático en el análisis óptico de muestras.
US11060138B1 (en) * 2020-01-17 2021-07-13 Element Biosciences, Inc. Nucleic acid sequencing systems
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
JP7233385B2 (ja) * 2020-01-20 2023-03-06 日本板硝子株式会社 反応処理装置
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
CN115243792A (zh) 2020-02-04 2022-10-25 加利福尼亚太平洋生物科学股份有限公司 流通池及其制造和使用方法
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
US12215379B2 (en) 2020-02-17 2025-02-04 10X Genomics, Inc. In situ analysis of chromatin interaction
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
AU2021225020A1 (en) 2020-02-21 2022-08-18 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
AU2021271637A1 (en) 2020-05-12 2022-12-08 Illumina Singapore Pte. Ltd. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
WO2021236625A1 (en) 2020-05-19 2021-11-25 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
AU2021288090A1 (en) 2020-06-10 2023-01-19 10X Genomics, Inc. Fluid delivery methods
WO2021252800A1 (en) 2020-06-11 2021-12-16 Nautilus Biotechnology, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
US20210403993A1 (en) 2020-06-30 2021-12-30 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
CN116018412A (zh) 2020-07-08 2023-04-25 Illumina公司 作为转座体载体的小珠
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
CN114688301A (zh) * 2020-12-31 2022-07-01 深圳市真迈生物科技有限公司 流动路径选择阀、流动池组件、液路系统及测序系统
WO2022068681A1 (zh) * 2020-09-29 2022-04-07 深圳市真迈生物科技有限公司 流动路径选择阀、系统、方法、存储介质及应用
CN114682310B (zh) * 2020-12-31 2023-12-05 深圳市真迈生物科技有限公司 液路系统、测序系统和方法
CN114321036A (zh) * 2020-09-29 2022-04-12 深圳市真迈生物科技有限公司 液路系统、检测系统和应用
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
JP2023549350A (ja) 2020-11-11 2023-11-24 ノーティラス・サブシディアリー・インコーポレイテッド 向上した結合及び検出特徴を有する親和性試薬
GB2603451B (en) * 2020-11-25 2024-10-02 Oxford Nanoimaging Ltd Reagent cartridge and measurement devices incorporating such cartridges
US12509717B2 (en) 2020-12-11 2025-12-30 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
CA3207097A1 (en) 2020-12-31 2022-07-07 Q-State Biosciences, Inc. Optical plate controller and reader
EP4271994A4 (en) 2020-12-31 2024-11-27 Q-State Biosciences, Inc. Calibrating and normalizing imager for optogenetics
JP2024501850A (ja) 2020-12-31 2024-01-16 キュー－ステート バイオサイエンシーズ， インコーポレイテッド 空間撮像およびビームホモジナイザを伴う顕微鏡
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4281775A1 (en) 2021-01-21 2023-11-29 Nautilus Subsidiary, Inc. Systems and methods for biomolecule preparation
EP4284945B1 (en) 2021-01-26 2024-10-23 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
EP4301873A1 (en) 2021-03-03 2024-01-10 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
EP4301516A4 (en) * 2021-03-03 2025-01-29 Illumina Inc REAGENT CARTRIDGES AND RELATED SYSTEMS AND METHODS FOR CONTROLLING THE TEMPERATURE OF A REAGENT
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
MX2023011659A (es) 2021-04-02 2023-10-11 Illumina Inc Modelo de aprendizaje automatico para detectar una burbuja dentro de un portaobjetos de muestra de nucleotidos para la secuenciacion.
WO2022256324A1 (en) 2021-06-01 2022-12-08 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
US20230084407A1 (en) 2021-06-02 2023-03-16 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
US20220403450A1 (en) 2021-06-03 2022-12-22 Illumina Software, Inc. Systems and methods for sequencing nucleotides using two optical channels
US20220411864A1 (en) 2021-06-23 2022-12-29 Illumina, Inc. Compositions, methods, kits, cartridges, and systems for sequencing reagents
KR20240025515A (ko) 2021-06-25 2024-02-27 일루미나, 인코포레이티드 선형 푸리에 기점
US20220414853A1 (en) 2021-06-25 2022-12-29 Illumina, Inc. Fiducials for use in registration of a patterned surface
CA3224402A1 (en) 2021-06-29 2023-01-05 Eric Jon Ojard Signal-to-noise-ratio metric for determining nucleotide-base calls and base-call quality
CA3224393A1 (en) 2021-06-29 2023-01-05 Mitchell A BEKRITSKY Machine-learning model for generating confidence classifications for genomic coordinates
US12105023B2 (en) 2021-06-30 2024-10-01 Quelltx, Inc. Multi-well plate readers and methods of their use
WO2023288225A1 (en) 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
EP4679300A2 (en) 2021-07-19 2026-01-14 Illumina, Inc. Intensity extraction with interpolation and adaptation for base calling
GB2626865A (en) 2021-07-21 2024-08-07 Element Biosciences Inc Optical systems for nucleic acid sequencing and methods thereof
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
ES2988400T3 (es) 2021-07-30 2024-11-20 10X Genomics Inc Métodos y composiciones para sincronizar reacciones in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
EP4381095A1 (en) 2021-08-03 2024-06-12 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US20230051226A1 (en) 2021-08-10 2023-02-16 Mgi Tech Co., Ltd. Flow cell imaging systems and methods, and flow cells and other substrates for use in the same
EP4446426A3 (en) 2021-08-16 2024-11-13 10x Genomics, Inc. Probes comprising a split barcode region and methods of use
WO2023034076A1 (en) 2021-08-31 2023-03-09 Illumina, Inc. Flow cell with enhanced well imaging resolution
WO2023034079A1 (en) 2021-09-01 2023-03-09 Illumina Software, Inc. Amplitude modulation for accelerated base calling
CA3227872A1 (en) 2021-09-09 2023-03-16 James Henry JOLY Characterization and localization of protein modifications
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
CN117561573A (zh) 2021-09-17 2024-02-13 因美纳有限公司 从碱基判读错误模式自动鉴定核苷酸测序中的故障来源
WO2023049558A1 (en) 2021-09-21 2023-03-30 Illumina, Inc. A graph reference genome and base-calling approach using imputed haplotypes
WO2023049212A2 (en) 2021-09-22 2023-03-30 Illumina, Inc. State-based base calling
WO2023049073A1 (en) 2021-09-22 2023-03-30 Nautilus Biotechnology, Inc. Methods and systems for determining polypeptide interactions
AU2022367166A1 (en) 2021-10-11 2024-04-04 Nautilus Subsidiary, Inc. Highly multiplexable analysis of proteins and proteomes
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
CN117581303A (zh) 2021-12-02 2024-02-20 因美纳有限公司 产生用于确定核苷酸碱基检出的簇特异性信号校正
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
WO2023122589A2 (en) * 2021-12-22 2023-06-29 Nautilus Subsidiary, Inc. Systems and methods for carrying out highly multiplexed bioanalyses
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
EP4457814A1 (en) 2021-12-29 2024-11-06 Illumina, Inc. Automatically switching variant analysis model versions for genomic analysis applications
WO2023129486A1 (en) * 2021-12-30 2023-07-06 Illumina, Inc. Imaging systems and related systems and methods
US12497653B2 (en) 2022-01-21 2025-12-16 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023158809A1 (en) 2022-02-17 2023-08-24 Illumina, Inc. Ai-driven enhancement of motion blurred sequencing images
US20230296516A1 (en) 2022-02-17 2023-09-21 Illumina, Inc. Ai-driven signal enhancement of sequencing images
AU2023223467A1 (en) 2022-02-23 2024-08-08 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
WO2023164492A1 (en) 2022-02-25 2023-08-31 Illumina, Inc. Machine-learning models for detecting and adjusting values for nucleotide methylation levels
EP4483372A1 (en) 2022-02-25 2025-01-01 Illumina, Inc. Calibration sequences for nucelotide sequencing
EP4500157A1 (en) 2022-03-29 2025-02-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
EP4505177A1 (en) 2022-04-01 2025-02-12 10x Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
CN119234045A (zh) 2022-04-06 2024-12-31 10X基因组学有限公司 用于多重细胞分析的方法
JP2025512975A (ja) 2022-04-07 2025-04-22 イルミナ インコーポレイテッド 改変シチジンデアミナーゼ及び使用方法
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4515547A1 (en) 2022-04-26 2025-03-05 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
US20230348967A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
WO2023220627A1 (en) 2022-05-10 2023-11-16 Illumina Software, Inc. Adaptive neural network for nucelotide sequencing
CN119317964A (zh) 2022-06-09 2025-01-14 因美纳有限公司 碱基检出对流通池倾斜的依赖
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
EP4544077A1 (en) 2022-06-21 2025-04-30 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
EP4544558A1 (en) 2022-06-24 2025-04-30 Illumina, Inc. Improving split-read alignment by intelligently identifying and scoring candidate split groups
EP4544555A1 (en) 2022-06-27 2025-04-30 Illumina, Inc. Generating and implementing a structural variation graph genome
IL317961A (en) 2022-06-27 2025-02-01 Illumina Inc Accelerators for genotype imputation mode
EP4544554A1 (en) 2022-06-27 2025-04-30 Illumina, Inc. Improved human leukocyte antigen (hla) genotyping
EP4562638A1 (en) 2022-07-26 2025-06-04 Illumina, Inc. Rapid single-cell multiomics processing using an executable file
EP4569119A1 (en) 2022-08-12 2025-06-18 10X Genomics, Inc. Puma1 polymerases and uses thereof
EP4511510B1 (en) 2022-08-16 2025-12-10 10X Genomics, Inc. Ap50 polymerases and uses thereof
EP4328569A1 (en) * 2022-08-23 2024-02-28 NanoTemper Technologies GmbH Measuring high protein concentrations
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024072799A1 (en) 2022-09-26 2024-04-04 Illumina, Inc. Flow cell based motion system calibration and control methods
WO2024073516A1 (en) 2022-09-29 2024-04-04 Illumina, Inc. A target-variant-reference panel for imputing target variants
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
EP4594482A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
EP4595059A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Machine-learning model for refining structural variant calls
US20250388894A1 (en) 2022-09-30 2025-12-25 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
EP4594481A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Helicase-cytidine deaminase complexes and methods of use
JP2025534929A (ja) 2022-10-05 2025-10-22 イルミナ インコーポレイテッド 機械学習アーキテクチャを利用した複数の配列決定パイプラインからの変異コールの統合
WO2024081649A1 (en) 2022-10-11 2024-04-18 Illumina, Inc. Detecting and correcting methylation values from methylation sequencing assays
EP4602344A1 (en) 2022-10-14 2025-08-20 10X Genomics, Inc. Methods for analysis of biological samples
US20240167081A1 (en) 2022-11-08 2024-05-23 10X Genomics,Inc. Immobilization methods and compositions for in situ detection
EP4573210A1 (en) 2022-11-16 2025-06-25 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
EP4619736A1 (en) 2022-11-18 2025-09-24 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
US20240177802A1 (en) 2022-11-30 2024-05-30 Illumina, Inc. Accurately predicting variants from methylation sequencing data
AU2022279500B1 (en) * 2022-12-01 2023-11-23 Provectus IP Pty Ltd Automated cell analyser and method
WO2024124073A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
EP4634285A1 (en) 2022-12-15 2025-10-22 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
WO2024130203A1 (en) 2022-12-16 2024-06-20 10X Genomics, Inc. Methods and compositions for assessing performance
EP4638789A1 (en) 2022-12-21 2025-10-29 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
US20240263219A1 (en) 2023-01-06 2024-08-08 10X Genomics, Inc. Methods and compositions for in situ analysis of variant sequences
US20240241110A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
AU2024212507A1 (en) 2023-01-27 2024-12-19 Illumina, Inc. Jitter correction image analysis
WO2024167954A1 (en) 2023-02-06 2024-08-15 Illumina, Inc. Determining and removing inter-cluster light interference
US20240344116A1 (en) 2023-03-24 2024-10-17 Nautilus Subsidiary, Inc. Transfer of nanoparticles to array surfaces
WO2024206848A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. Tandem repeat genotyping
AU2024266370A1 (en) 2023-05-03 2025-01-16 Illumina, Inc. Machine learning model for recalibrating genotype calls from existing sequencing data files
US20240368678A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
US20240404624A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Structural variant alignment and variant calling by utilizing a structural-variant reference genome
WO2024249973A2 (en) 2023-06-02 2024-12-05 Illumina, Inc. Linking human genes to clinical phenotypes using graph neural networks
WO2025006565A1 (en) 2023-06-27 2025-01-02 Illumina, Inc. Variant calling with methylation-level estimation
WO2025006464A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with alternative scatterplots
WO2025006460A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with modified bases
WO2025006874A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Machine-learning model for recalibrating genotype calls corresponding to germline variants and somatic mosaic variants
WO2025006466A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with four labeled nucleotides
CN120936702A (zh) * 2023-07-05 2025-11-11 武汉华大智造科技有限公司 用于基因测序的流路系统及其控制方法和基因测序仪
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
US20250052565A1 (en) 2023-08-11 2025-02-13 Illumina, Inc. Optical alignment targets
WO2025043159A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
WO2025054389A1 (en) 2023-09-07 2025-03-13 Illumina, Inc. Identification of methylated cytosine using landmarks
EP4520821A1 (en) 2023-09-08 2025-03-12 The Regents Of The University Of Michigan Microrna-derived rnas and polypeptides and uses thereof
WO2025072793A1 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2025072870A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Tracking and modifying cluster location on nucleotide-sample slides in real time
WO2025072833A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Predicting insert lengths using primary analysis metrics
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025090883A1 (en) 2023-10-27 2025-05-01 Illumina, Inc. Detecting variants in nucleotide sequences based on haplotype diversity
WO2025111463A1 (en) 2023-11-22 2025-05-30 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
WO2025129133A1 (en) 2023-12-15 2025-06-19 Illumina, Inc. Minimal residual disease (mrd) models for determining likelihoods or probabilities of a subject comprising cancer
WO2025136998A1 (en) 2023-12-18 2025-06-26 Illumina, Inc. Using machine learning models for detecting minimum residual disease (mrd) in a subject
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
US20250210137A1 (en) 2023-12-20 2025-06-26 Illumina, Inc. Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality
EP4578960A1 (en) 2023-12-20 2025-07-02 10x Genomics, Inc. In situ detection of copy number variations in biological samples
WO2025137166A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
US20250210141A1 (en) 2023-12-21 2025-06-26 Illumina, Inc. Enhanced mapping and alignment of nucleotide reads utilizing an improved haplotype data structure with allele-variant differences
WO2025160089A1 (en) 2024-01-26 2025-07-31 Illumina, Inc. Custom multigenome reference construction for improved sequencing analysis of genomic samples
WO2025174774A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Determining offline corrections for sequence specific errors caused by low complexity nucleotide sequences
WO2025174708A1 (en) 2024-02-13 2025-08-21 Illumina, Inc. Design and method for cross-sequencing platform compatibility of flow cells
WO2025184234A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. A personalized haplotype database for improved mapping and alignment of nucleotide reads and improved genotype calling
WO2025193747A1 (en) 2024-03-12 2025-09-18 Illumina, Inc. Machine-learning models for ordering and expediting sequencing tasks or corresponding nucleotide-sample slides
WO2025194033A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
US20250305034A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
WO2025240241A1 (en) 2024-05-13 2025-11-20 Illumina, Inc. Modifying sequencing cycles during a sequencing run to meet customized coverage estimations for a target genomic region
WO2025240924A1 (en) 2024-05-17 2025-11-20 Illumina, Inc. Blind equalization systems for base calling applications
WO2025240918A1 (en) 2024-05-17 2025-11-20 10X Genomics, Inc. Systems and methods for generating codebooks
WO2025250996A2 (en) 2024-05-31 2025-12-04 Illumina, Inc. Call generation and recalibration models for implementing personalized diploid reference haplotypes in genotype calling
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling
WO2025264702A1 (en) 2024-06-18 2025-12-26 10X Genomics, Inc. 3d hydrogels for nucleic acid sequencing
WO2026006774A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2026006771A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Cluster-filtering scores

Family Cites Families (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4160263A (en) 1978-05-15 1979-07-03 George R. Cogar Dual or multiple objective video microscope for superimposing spaced images
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US6953676B1 (en) * 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
DE19536903C2 (de) * 1995-10-04 1998-09-10 Boehringer Ingelheim Int Vorrichtung zum Haltern eines fluidischen Bauteils
US5803117A (en) 1996-06-10 1998-09-08 Rheodyne, L.P. Multi-route full sweep selection valve
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
EP1498494A3 (en) 1997-04-01 2007-06-20 Solexa Ltd. Method of nucleic acid sequencing
US6399936B1 (en) 1997-12-01 2002-06-04 New Dimension Research Instrument, Inc. Optical confocal device having a common light directing means
US20030036855A1 (en) * 1998-03-16 2003-02-20 Praelux Incorporated, A Corporation Of New Jersey Method and apparatus for screening chemical compounds
US6185030B1 (en) * 1998-03-20 2001-02-06 James W. Overbeck Wide field of view and high speed scanning microscopy
US6012488A (en) 1998-09-17 2000-01-11 Rheodyne, L.P. Segmenting valve
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6326612B1 (en) * 1998-10-13 2001-12-04 Texas Instruments Incorporated System and method for optical sensing utilizing a portable, detachable sensor cartridge
US6424404B1 (en) 1999-01-11 2002-07-23 Kenneth C. Johnson Multi-stage microlens array
ATE553219T1 (de) 1999-04-20 2012-04-15 Illumina Inc Erkennung von nukleinsäurereaktionen auf bead- arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US20030215821A1 (en) 1999-04-20 2003-11-20 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US6320174B1 (en) 1999-11-16 2001-11-20 Ikonisys Inc. Composing microscope
US20030091477A1 (en) * 1999-12-22 2003-05-15 Paul Eric A. Flow-thru chip cartridge, chip holder, system & method thereof
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US6526658B1 (en) * 2000-05-23 2003-03-04 Silverbrook Research Pty Ltd Method of manufacture of an ink jet printhead having a moving nozzle with an externally arranged actuator
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
US6603546B1 (en) 2000-07-21 2003-08-05 I.S.S. (Usa) Inc. Rapid high throughput spectrometer and method
US6374684B1 (en) 2000-08-25 2002-04-23 Cepheid Fluid control and processing system
US6875973B2 (en) * 2000-08-25 2005-04-05 Amnis Corporation Auto focus for a flow imaging system
US7514046B2 (en) * 2000-10-31 2009-04-07 Caliper Life Sciences, Inc. Methods and systems for processing microscale devices for reuse
EP1343973B2 (en) * 2000-11-16 2020-09-16 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
JP2002219849A (ja) 2000-11-27 2002-08-06 Riso Kagaku Corp 孔版印刷装置
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US7864369B2 (en) 2001-03-19 2011-01-04 Dmetrix, Inc. Large-area imaging by concatenation with array microscope
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US7116437B2 (en) 2001-09-14 2006-10-03 Dmetrix Inc. Inter-objective baffle system
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
US6982166B2 (en) 2002-05-16 2006-01-03 Applera Corporation Lens assembly for biological testing
US7193775B2 (en) 2002-05-30 2007-03-20 Dmetrix, Inc. EPI-illumination system for an array microscope
US6987259B2 (en) 2002-05-30 2006-01-17 Dmetrix, Inc. Imaging system with an integrated source and detector array
US7312432B2 (en) 2002-07-08 2007-12-25 Dmetrix, Inc. Single axis illumination for multi-axis imaging system
US7582470B2 (en) * 2002-07-31 2009-09-01 Gen-Probe Incorporated Device for amplifying and detecting a target nucleic acid
ES2550513T3 (es) 2002-08-23 2015-11-10 Illumina Cambridge Limited Nucleótidos modificados para secuenciación de polinucleótidos
US6950241B1 (en) 2002-09-18 2005-09-27 Dmetrix, Inc. Miniature microscope objective for an array microscope
US7388714B2 (en) 2002-09-18 2008-06-17 Dmetrix, Inc. Independent focus compensation for a multi-axis imaging system
US7130115B2 (en) 2002-09-23 2006-10-31 Dhetrix, Inc. Multi-mode scanning imaging system
US7034317B2 (en) 2002-12-17 2006-04-25 Dmetrix, Inc. Method and apparatus for limiting scanning imaging array data to characteristics of interest
US20050221281A1 (en) * 2003-01-08 2005-10-06 Ho Winston Z Self-contained microfluidic biochip and apparatus
EP1594981B1 (en) 2003-01-29 2010-03-17 454 Corporation Double ended sequencing
US7330574B2 (en) 2003-05-08 2008-02-12 Ometrix, Inc. Best-focus estimation by lateral scanning
US7148043B2 (en) * 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
WO2005065814A1 (en) 2004-01-07 2005-07-21 Solexa Limited Modified molecular arrays
ATE353313T1 (de) 2004-05-22 2007-02-15 Agilent Technologies Inc Bauteil eines mikrofluidischen ventils
WO2006085948A2 (en) * 2004-07-01 2006-08-17 Cornell Research Foundation, Inc. Real-time pcr detection of microorganisms using an integrated microfluidics platform
WO2006018044A1 (en) 2004-08-18 2006-02-23 Agilent Technologies, Inc. Microfluidic assembly with coupled microfluidic devices
US7302146B2 (en) 2004-09-17 2007-11-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
CA2587085C (en) * 2004-10-27 2017-08-08 Cepheid Closed-system multi-stage nucleic acid amplification reactions
FI20055043A0 (fi) * 2005-01-31 2005-01-31 Wallac Oy Sovitelma mikrotiterkaivojen kuvausjärjestelmässä
US7507575B2 (en) * 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
DK1907571T3 (en) 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
WO2007021820A2 (en) 2005-08-11 2007-02-22 Eksigent Technologies, Llc Methods for measuring biochemical reactions
JP5058483B2 (ja) * 2005-09-14 2012-10-24 オリンパス株式会社 生体試料の長期間ないし連続的検出方法
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
JP4742242B2 (ja) * 2005-09-30 2011-08-10 カシオ計算機株式会社 撮像装置及びそのプログラム
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US7329860B2 (en) 2005-11-23 2008-02-12 Illumina, Inc. Confocal imaging methods and apparatus
WO2008030631A2 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
US20080009420A1 (en) 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
CA2648149A1 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US8900828B2 (en) * 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
US8137626B2 (en) * 2006-05-19 2012-03-20 California Institute Of Technology Fluorescence detector, filter device and related methods
US7641860B2 (en) 2006-06-01 2010-01-05 Nanotek, Llc Modular and reconfigurable multi-stage microreactor cartridge apparatus
US7586674B2 (en) * 2006-10-17 2009-09-08 Hnu-Photonics Compuscope
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
BRPI0717385A2 (pt) * 2006-10-24 2013-10-15 Koninkl Philips Electronics Nv Dispositivo, aparelho e método para formar imagem de um objeto
US7813013B2 (en) 2006-11-21 2010-10-12 Illumina, Inc. Hexagonal site line scanning method and system
EP2126765B1 (en) * 2007-01-26 2011-08-24 Illumina Inc. Nucleic acid sequencing system and method
US9322063B2 (en) 2007-01-26 2016-04-26 Illumina, Inc. Efficient biomolecule recycling method and system
US8298763B2 (en) 2007-03-02 2012-10-30 Lawrence Livermore National Security, Llc Automated high-throughput flow-through real-time diagnostic system
US8039817B2 (en) * 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
DE102008002509A1 (de) * 2008-06-18 2009-12-31 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Stopped-Flow-Chip
US20110224574A1 (en) * 2008-11-13 2011-09-15 Sadler John W Methods and systems for tissue processing and imaging
WO2010062913A2 (en) 2008-11-26 2010-06-03 Illumina, Inc. Methods and systems for analysis of sequencing data
US20100157086A1 (en) * 2008-12-15 2010-06-24 Illumina, Inc Dynamic autofocus method and system for assay imager
US8673627B2 (en) * 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8304704B2 (en) * 2009-07-27 2012-11-06 Sensovation Ag Method and apparatus for autofocus using a light source pattern and means for masking the light source pattern
WO2011026141A2 (en) * 2009-08-31 2011-03-03 Life Technologies Corporation Fast-indexing filter wheel and method of use
KR101814056B1 (ko) 2009-12-01 2018-01-02 옥스포드 나노포어 테크놀로지즈 리미티드 생화학적 분석 기구
CA3028780C (en) * 2009-12-07 2022-05-31 Meso Scale Technologies, Llc Assay cartridges and methods of using the same
AU2011293269B2 (en) * 2010-08-27 2015-10-01 The Board Of Trustees Of The Leland Stanford Junior University Microscopy imaging device with advanced imaging properties
US9593370B2 (en) 2010-10-01 2017-03-14 Oxford Nanopore Technologies Ltd. Biochemical analysis apparatus and rotary valve
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
IN2014DN07992A (enExample) * 2012-04-03 2015-05-01 Illumina Inc

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KR20210080618A (ko) 2021-06-30
AU2013243998B2 (en) 2016-07-28
US20170246635A1 (en) 2017-08-31
CN204832037U (zh) 2015-12-02
MX337140B (es) 2016-02-12
AU2021200408B2 (en) 2023-04-06
ES2949570T3 (es) 2023-09-29
IL268205A (en) 2019-08-29
IN2014DN07992A (enExample) 2015-05-01
US20130260372A1 (en) 2013-10-03
AU2019200400A1 (en) 2019-02-07
US9650669B2 (en) 2017-05-16
AU2013243998A1 (en) 2014-10-09
US9193996B2 (en) 2015-11-24
BR112014024789B1 (pt) 2021-05-25

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