CA2996888A1 - Engineered crispr-cas9 nucleases - Google Patents

Engineered crispr-cas9 nucleases Download PDF

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Publication number
CA2996888A1
CA2996888A1 CA2996888A CA2996888A CA2996888A1 CA 2996888 A1 CA2996888 A1 CA 2996888A1 CA 2996888 A CA2996888 A CA 2996888A CA 2996888 A CA2996888 A CA 2996888A CA 2996888 A1 CA2996888 A1 CA 2996888A1
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CA
Canada
Prior art keywords
spcas9
protein
mutations
cell
target
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Pending
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CA2996888A
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English (en)
French (fr)
Inventor
J. Keith Joung
Benjamin KLEINSTIVER
Vikram PATTANAYAK
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General Hospital Corp
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General Hospital Corp
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Priority claimed from US15/015,947 external-priority patent/US9512446B1/en
Application filed by General Hospital Corp filed Critical General Hospital Corp
Publication of CA2996888A1 publication Critical patent/CA2996888A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2996888A 2015-08-28 2016-08-26 Engineered crispr-cas9 nucleases Pending CA2996888A1 (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US201562211553P 2015-08-28 2015-08-28
US62/211,553 2015-08-28
US201562216033P 2015-09-09 2015-09-09
US62/216,033 2015-09-09
US201562258280P 2015-11-20 2015-11-20
US62/258,280 2015-11-20
US201562271938P 2015-12-28 2015-12-28
US62/271,938 2015-12-28
US15/015,947 US9512446B1 (en) 2015-08-28 2016-02-04 Engineered CRISPR-Cas9 nucleases
US15/015,947 2016-02-04
PCT/US2016/049147 WO2017040348A1 (en) 2015-08-28 2016-08-26 Engineered crispr-cas9 nucleases

Publications (1)

Publication Number Publication Date
CA2996888A1 true CA2996888A1 (en) 2017-03-09

Family

ID=58188120

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2996888A Pending CA2996888A1 (en) 2015-08-28 2016-08-26 Engineered crispr-cas9 nucleases

Country Status (8)

Country Link
EP (2) EP4036236A1 (enExample)
JP (6) JP6799586B2 (enExample)
KR (2) KR20240090567A (enExample)
CN (2) CN108350449B (enExample)
AU (2) AU2016316845B2 (enExample)
CA (1) CA2996888A1 (enExample)
HK (1) HK1257676A1 (enExample)
WO (1) WO2017040348A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4253549A3 (en) * 2018-09-19 2023-12-06 The University of Hong Kong Improved high-throughput combinatorial genetic modification system and optimized cas9 enzyme variants

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