CA2996219C - Emulsion chemistry and assays for encapsulated droplets - Google Patents

Emulsion chemistry and assays for encapsulated droplets Download PDF

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Publication number
CA2996219C
CA2996219C CA2996219A CA2996219A CA2996219C CA 2996219 C CA2996219 C CA 2996219C CA 2996219 A CA2996219 A CA 2996219A CA 2996219 A CA2996219 A CA 2996219A CA 2996219 C CA2996219 C CA 2996219C
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Prior art keywords
capsules
surfactant
phase
droplets
emulsion
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CA2996219A
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English (en)
French (fr)
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CA2996219A1 (en
Inventor
Amy L. Hiddessen
Benjamin J. Hindson
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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Priority to CA3202170A priority Critical patent/CA3202170A1/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6846Common amplification features
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
CA2996219A 2010-03-02 2011-03-02 Emulsion chemistry and assays for encapsulated droplets Active CA2996219C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3202170A CA3202170A1 (en) 2010-03-02 2011-03-02 Emulsion chemistry and assays for encapsulated droplets

Applications Claiming Priority (21)

Application Number Priority Date Filing Date Title
US30984510P 2010-03-02 2010-03-02
US61/309,845 2010-03-02
US34121810P 2010-03-25 2010-03-25
US31763510P 2010-03-25 2010-03-25
US61/317,635 2010-03-25
US61/341,218 2010-03-25
US38098110P 2010-09-08 2010-09-08
US61/380,981 2010-09-08
US40910610P 2010-11-01 2010-11-01
US61/409,106 2010-11-01
US40947310P 2010-11-02 2010-11-02
US61/409,473 2010-11-02
US41076910P 2010-11-05 2010-11-05
US61/410,769 2010-11-05
US41724110P 2010-11-25 2010-11-25
US61/417,241 2010-11-25
US12/976,816 2010-12-22
US12/976,827 US9598725B2 (en) 2010-03-02 2010-12-22 Emulsion chemistry for encapsulated droplets
US12/976,816 US8399198B2 (en) 2010-03-02 2010-12-22 Assays with droplets transformed into capsules
US12/976,827 2010-12-22
CA2824444A CA2824444C (en) 2010-03-02 2011-03-02 Emulsion chemistry and assays for encapsulated droplets

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2824444A Division CA2824444C (en) 2010-03-02 2011-03-02 Emulsion chemistry and assays for encapsulated droplets

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3202170A Division CA3202170A1 (en) 2010-03-02 2011-03-02 Emulsion chemistry and assays for encapsulated droplets

Publications (2)

Publication Number Publication Date
CA2996219A1 CA2996219A1 (en) 2011-09-09
CA2996219C true CA2996219C (en) 2023-08-08

Family

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Family Applications (3)

Application Number Title Priority Date Filing Date
CA2996219A Active CA2996219C (en) 2010-03-02 2011-03-02 Emulsion chemistry and assays for encapsulated droplets
CA3202170A Pending CA3202170A1 (en) 2010-03-02 2011-03-02 Emulsion chemistry and assays for encapsulated droplets
CA2824444A Active CA2824444C (en) 2010-03-02 2011-03-02 Emulsion chemistry and assays for encapsulated droplets

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CA3202170A Pending CA3202170A1 (en) 2010-03-02 2011-03-02 Emulsion chemistry and assays for encapsulated droplets
CA2824444A Active CA2824444C (en) 2010-03-02 2011-03-02 Emulsion chemistry and assays for encapsulated droplets

Country Status (5)

Country Link
US (6) US8399198B2 (cg-RX-API-DMAC7.html)
EP (4) EP3636741B1 (cg-RX-API-DMAC7.html)
JP (2) JP5963683B2 (cg-RX-API-DMAC7.html)
CA (3) CA2996219C (cg-RX-API-DMAC7.html)
WO (1) WO2011109546A1 (cg-RX-API-DMAC7.html)

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