CA2945661C - Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides - Google Patents
Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides Download PDFInfo
- Publication number
- CA2945661C CA2945661C CA2945661A CA2945661A CA2945661C CA 2945661 C CA2945661 C CA 2945661C CA 2945661 A CA2945661 A CA 2945661A CA 2945661 A CA2945661 A CA 2945661A CA 2945661 C CA2945661 C CA 2945661C
- Authority
- CA
- Canada
- Prior art keywords
- bacterium
- gene
- lactose
- protein
- fucosyltransferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
- C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
- C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01069—Galactoside 2-alpha-L-fucosyltransferase (2.4.1.69)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01086—Glucosaminylgalactosylglucosylceramide beta-galactosyltransferase (2.4.1.86)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01149—N-Acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase (2.4.1.149)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461993742P | 2014-05-15 | 2014-05-15 | |
| US61/993,742 | 2014-05-15 | ||
| PCT/US2015/030823 WO2015175801A1 (en) | 2014-05-15 | 2015-05-14 | Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2945661A1 CA2945661A1 (en) | 2015-11-19 |
| CA2945661C true CA2945661C (en) | 2023-10-17 |
Family
ID=54480689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2945661A Active CA2945661C (en) | 2014-05-15 | 2015-05-14 | Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US11046984B2 (enExample) |
| EP (2) | EP3083938B1 (enExample) |
| JP (3) | JP2017515455A (enExample) |
| KR (2) | KR20230113650A (enExample) |
| CN (1) | CN106795484B (enExample) |
| AU (1) | AU2015259088B2 (enExample) |
| CA (1) | CA2945661C (enExample) |
| ES (1) | ES2962258T3 (enExample) |
| HU (1) | HUE064860T2 (enExample) |
| MX (1) | MX2016014807A (enExample) |
| SG (2) | SG11201609366TA (enExample) |
| WO (1) | WO2015175801A1 (enExample) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6129821B2 (ja) | 2011-05-13 | 2017-05-17 | グリコシン リミテッド ライアビリティー カンパニー | プレバイオティクスとしての、精製された２’−フコシルラクトース、３−フコシルラクトース、およびラクトジフコテトラオースの使用 |
| US9029136B2 (en) * | 2012-07-25 | 2015-05-12 | Glycosyn LLC | Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides |
| DE14769797T1 (de) | 2013-03-14 | 2016-06-23 | Glycosyn LLC | Mikroorganismen und Verfahren zur Herstellung sialylierter und N-acetylglucosamin-haltiger Oligosaccharide |
| SG11201609366TA (en) | 2014-05-15 | 2016-12-29 | Glycosyn LLC | Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides |
| EP3390652B1 (en) * | 2015-12-18 | 2024-12-04 | Glycom A/S | Fermentative production of oligosaccharides |
| WO2017106864A1 (en) * | 2015-12-18 | 2017-06-22 | The Regents Of The University Of California | Te2ft enzyme for enzymatic synthesis of alpha1-2-fucosides |
| CN106190937B9 (zh) * | 2016-07-18 | 2020-12-01 | 南开大学 | 一种构建重组大肠杆菌生物合成2’-岩藻乳糖的方法 |
| DK3375291T3 (da) * | 2017-03-17 | 2022-10-31 | Chr Hansen Hmo Gmbh | Fremgangsmåde til hæmning af isomerisering af et reducerende saccharid efter termisk behandling |
| EP3425052A1 (en) * | 2017-07-07 | 2019-01-09 | Jennewein Biotechnologie GmbH | Fucosyltransferases and their use in producing fucosylated oligosaccharides |
| EP3438122A1 (en) * | 2017-08-01 | 2019-02-06 | OligoScience Biotechnology GmbH | Microorganism for producing human milk oligosaccharide |
| KR101953375B1 (ko) * | 2017-11-20 | 2019-02-28 | 고려대학교 산학협력단 | 다양하고 신규한 퓨코실 올리고당의 제조법 및 이의 용도 |
| CN109402020B (zh) * | 2018-11-30 | 2020-08-04 | 江南大学 | 一株高产精氨酸氨肽酶的瑞士乳杆菌及其应用 |
| CN109735479B (zh) * | 2019-01-30 | 2022-04-01 | 光明乳业股份有限公司 | 一种合成2’-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法与应用 |
| RS65200B1 (sr) * | 2019-03-04 | 2024-03-29 | Aurealis Therapeutics AG | Prokariotski ekspresioni sistem inducibilan hloridom |
| WO2020246585A1 (ja) | 2019-06-05 | 2020-12-10 | 森永乳業株式会社 | 栄養組成物 |
| JP2023506287A (ja) * | 2019-12-17 | 2023-02-15 | インバイオス エン．フェー． | ラクトースを変換するアルファ－１，２－フコシルトランスフェラーゼ酵素 |
| CN111218488B (zh) * | 2020-02-10 | 2022-11-15 | 中国科学院上海高等研究院 | 一种利用大肠杆菌生产2’-岩藻糖基乳糖的方法 |
| CN111471606B (zh) * | 2020-03-17 | 2022-03-08 | 山东大学 | 一株优化的高产岩藻糖基乳糖的酿酒酵母菌株及其应用 |
| CN111471605B (zh) * | 2020-03-17 | 2022-03-08 | 山东大学 | 一株高产岩藻糖基乳糖的酿酒酵母工程菌株及其应用 |
| KR102719035B1 (ko) * | 2020-04-20 | 2024-10-18 | 주식회사 리비옴 | 혈관 작동성 장 펩티드를 발현하는 미생물, 및 그의 용도 |
| EP3954769A1 (en) * | 2020-08-10 | 2022-02-16 | Inbiose N.V. | Production of oligosaccharide mixtures by a cell |
| US11331329B2 (en) | 2020-05-13 | 2022-05-17 | Glycosyn LLC | Fucosylated oligosaccharides for prevention of coronavirus infection |
| CA3178736A1 (en) | 2020-05-13 | 2021-11-18 | Ardythe L. Morrow | 2'-fucosyllactose for the prevention and treatment of coronavirus-induced inflammation |
| CN113969256A (zh) * | 2020-07-24 | 2022-01-25 | 上海交通大学 | 一种生产n-乙酰氨基葡萄糖的菌株及其构建方法和应用 |
| EP4192944A1 (en) | 2020-08-10 | 2023-06-14 | Inbiose N.V. | Production of oligosaccharide mixtures by a cell |
| EP4200318A4 (en) * | 2020-08-19 | 2024-06-12 | Conagen Inc. | BIOSYNTHETIC PRODUCTION OF 2-FUCOSYLLACTOSE |
| KR102793833B1 (ko) * | 2020-12-31 | 2025-04-11 | 주식회사 삼양사 | 푸코오스 전이효소를 발현하는 재조합 미생물 및 이를 이용한 2’-푸코실락토오스 제조방법 |
| KR102633804B1 (ko) * | 2020-12-31 | 2024-02-05 | 주식회사 삼양사 | 재조합 바실러스 속 미생물 및 이를 이용한 모유올리고당 제조방법 |
| WO2023182970A1 (en) * | 2021-02-19 | 2023-09-28 | The Regents Of The University Of California | Methods for production of fucosylated oligosaccharides in recombinant cell culture |
| WO2022176994A1 (ja) * | 2021-02-19 | 2022-08-25 | 協和発酵バイオ株式会社 | 改変されたα１，２－フコシルトランスフェラーゼ活性を有する蛋白質及びフコース含有糖質の製造法 |
| DK181242B1 (en) | 2021-05-17 | 2023-05-30 | Dsm Ip Assets Bv | GENETICALLY ENGINEERED CELLS COMPRISING A RECOMBINANT NUCLEIC ACID SEQUNCE ENCODING AN α-1,2-FUCOSYLTRANSFERASE CAPABLE OF PRODUCING LNFP-I, NUCLEIC ACID SEQUENCES ENCODING SAME AND METHODS FOR USE OF SAME |
| EP4370664A1 (en) * | 2021-07-16 | 2024-05-22 | Inbiose N.V. | Lacto-n-biose converting fucosyltransferases |
| CN113817701B (zh) * | 2021-09-30 | 2023-08-18 | 中南大学 | α-1,2-岩藻糖基转移酶突变体及其应用 |
| CN113832092B (zh) * | 2021-10-20 | 2023-10-03 | 江南大学 | 一种提高乳酰-n-岩藻五糖产量的基因工程菌及其生产方法 |
| CN116286919A (zh) * | 2021-12-03 | 2023-06-23 | 虹摹生物科技(上海)有限公司 | 基因工程菌及利用其制备岩藻糖基化寡糖的方法 |
| KR20240017427A (ko) * | 2022-07-29 | 2024-02-08 | 주식회사 진켐 | 3-푸코실락토스 생산용 유전자 조작 미생물 및 이를 이용한 방법 |
| CN116042671B (zh) * | 2023-02-14 | 2024-11-22 | 华熙生物科技股份有限公司 | 一种用于制备母乳寡糖的核酸分子、表达载体和微生物 |
| CN116769808B (zh) * | 2023-02-27 | 2025-06-10 | 江南大学 | 一种专一生产2′-岩藻糖基乳糖的菌株及应用 |
| WO2025087087A1 (zh) * | 2023-10-23 | 2025-05-01 | 山东恒鲁生物科技有限公司 | 一种岩藻糖基转移酶多肽及其应用 |
| CN119614406B (zh) * | 2023-10-23 | 2025-09-19 | 恒睿（青岛）生物科技有限公司 | 一种克鲁维酵母基因修饰细胞 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2796082B1 (fr) * | 1999-07-07 | 2003-06-27 | Centre Nat Rech Scient | Procede de production d'oligosaccharides |
| JP2012503491A (ja) * | 2008-09-25 | 2012-02-09 | グリコシン インコーポレイテッド | プロバイオティック酵母を操作するための組成物および方法 |
| ES2439507T3 (es) * | 2011-01-20 | 2014-01-23 | Jennewein Biotechnologie Gmbh | Fucosiltransferasas novedosas y sus aplicaciones |
| DE12746649T1 (de) * | 2011-02-16 | 2016-09-29 | Glycosyn LLC | Biosynthese menschlicher milcholigosaccaride in manipulierten Bakterien |
| US9029136B2 (en) * | 2012-07-25 | 2015-05-12 | Glycosyn LLC | Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides |
| ES2715010T3 (es) * | 2014-03-31 | 2019-05-31 | Jennewein Biotechnologie Gmbh | Fermentación total de oligosacáridos |
| SG11201609366TA (en) | 2014-05-15 | 2016-12-29 | Glycosyn LLC | Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides |
-
2015
- 2015-05-14 SG SG11201609366TA patent/SG11201609366TA/en unknown
- 2015-05-14 KR KR1020237024435A patent/KR20230113650A/ko not_active Ceased
- 2015-05-14 EP EP15792578.5A patent/EP3083938B1/en active Active
- 2015-05-14 HU HUE15792578A patent/HUE064860T2/hu unknown
- 2015-05-14 CN CN201580028020.2A patent/CN106795484B/zh active Active
- 2015-05-14 SG SG10202010599TA patent/SG10202010599TA/en unknown
- 2015-05-14 CA CA2945661A patent/CA2945661C/en active Active
- 2015-05-14 EP EP23203630.1A patent/EP4293033A3/en not_active Withdrawn
- 2015-05-14 ES ES15792578T patent/ES2962258T3/es active Active
- 2015-05-14 AU AU2015259088A patent/AU2015259088B2/en not_active Ceased
- 2015-05-14 JP JP2016549476A patent/JP2017515455A/ja not_active Withdrawn
- 2015-05-14 WO PCT/US2015/030823 patent/WO2015175801A1/en not_active Ceased
- 2015-05-14 US US15/307,914 patent/US11046984B2/en not_active Expired - Fee Related
- 2015-05-14 KR KR1020167033842A patent/KR20170035835A/ko not_active Ceased
- 2015-05-14 MX MX2016014807A patent/MX2016014807A/es unknown
-
2020
- 2020-05-18 JP JP2020086439A patent/JP2020188761A/ja active Pending
-
2021
- 2021-06-22 US US17/354,819 patent/US11643675B2/en active Active
-
2022
- 2022-09-29 JP JP2022156260A patent/JP2022173457A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20220056497A1 (en) | 2022-02-24 |
| JP2022173457A (ja) | 2022-11-18 |
| EP4293033A2 (en) | 2023-12-20 |
| EP4293033A3 (en) | 2024-04-10 |
| ES2962258T3 (es) | 2024-03-18 |
| SG11201609366TA (en) | 2016-12-29 |
| MX2016014807A (es) | 2017-05-10 |
| EP3083938C0 (en) | 2023-11-08 |
| CN106795484A (zh) | 2017-05-31 |
| EP3083938A1 (en) | 2016-10-26 |
| KR20230113650A (ko) | 2023-07-31 |
| SG10202010599TA (en) | 2020-11-27 |
| CA2945661A1 (en) | 2015-11-19 |
| KR20170035835A (ko) | 2017-03-31 |
| EP3083938A4 (en) | 2017-05-31 |
| JP2017515455A (ja) | 2017-06-15 |
| WO2015175801A9 (en) | 2016-10-20 |
| EP3083938B1 (en) | 2023-11-08 |
| AU2015259088A2 (en) | 2018-06-28 |
| JP2020188761A (ja) | 2020-11-26 |
| US11643675B2 (en) | 2023-05-09 |
| NZ722185A (en) | 2023-12-22 |
| AU2015259088A1 (en) | 2016-08-04 |
| US11046984B2 (en) | 2021-06-29 |
| AU2015259088B2 (en) | 2021-11-11 |
| US20170081353A1 (en) | 2017-03-23 |
| WO2015175801A1 (en) | 2015-11-19 |
| HUE064860T2 (hu) | 2024-04-28 |
| CN106795484B (zh) | 2022-09-09 |
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