AU2015259088B2 - Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides - Google Patents

Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides Download PDF

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Publication number
AU2015259088B2
AU2015259088B2 AU2015259088A AU2015259088A AU2015259088B2 AU 2015259088 B2 AU2015259088 B2 AU 2015259088B2 AU 2015259088 A AU2015259088 A AU 2015259088A AU 2015259088 A AU2015259088 A AU 2015259088A AU 2015259088 B2 AU2015259088 B2 AU 2015259088B2
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AU
Australia
Prior art keywords
seq
bacterium
gene
lactose
fucosyltransferase
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Ceased
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AU2015259088A
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AU2015259088A2 (en
AU2015259088A1 (en
Inventor
Matthew Ian Heidtman
John M. Mccoy
Massimo MERIGHI
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Glycosyn LLC
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Glycosyn LLC
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
    • C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
    • C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01069—Galactoside 2-alpha-L-fucosyltransferase (2.4.1.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01086—Glucosaminylgalactosylglucosylceramide beta-galactosyltransferase (2.4.1.86)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01149—N-Acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase (2.4.1.149)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AU2015259088A 2014-05-15 2015-05-14 Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides Ceased AU2015259088B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461993742P 2014-05-15 2014-05-15
US61/993,742 2014-05-15
PCT/US2015/030823 WO2015175801A1 (en) 2014-05-15 2015-05-14 Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides

Publications (3)

Publication Number Publication Date
AU2015259088A1 AU2015259088A1 (en) 2016-08-04
AU2015259088A2 AU2015259088A2 (en) 2018-06-28
AU2015259088B2 true AU2015259088B2 (en) 2021-11-11

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AU2015259088A Ceased AU2015259088B2 (en) 2014-05-15 2015-05-14 Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides

Country Status (12)

Country Link
US (2) US11046984B2 (enExample)
EP (2) EP3083938B1 (enExample)
JP (3) JP2017515455A (enExample)
KR (2) KR20230113650A (enExample)
CN (1) CN106795484B (enExample)
AU (1) AU2015259088B2 (enExample)
CA (1) CA2945661C (enExample)
ES (1) ES2962258T3 (enExample)
HU (1) HUE064860T2 (enExample)
MX (1) MX2016014807A (enExample)
SG (2) SG11201609366TA (enExample)
WO (1) WO2015175801A1 (enExample)

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US9029136B2 (en) * 2012-07-25 2015-05-12 Glycosyn LLC Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides
DE14769797T1 (de) 2013-03-14 2016-06-23 Glycosyn LLC Mikroorganismen und Verfahren zur Herstellung sialylierter und N-acetylglucosamin-haltiger Oligosaccharide
SG11201609366TA (en) 2014-05-15 2016-12-29 Glycosyn LLC Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides
EP3390652B1 (en) * 2015-12-18 2024-12-04 Glycom A/S Fermentative production of oligosaccharides
WO2017106864A1 (en) * 2015-12-18 2017-06-22 The Regents Of The University Of California Te2ft enzyme for enzymatic synthesis of alpha1-2-fucosides
CN106190937B9 (zh) * 2016-07-18 2020-12-01 南开大学 一种构建重组大肠杆菌生物合成2’-岩藻乳糖的方法
DK3375291T3 (da) * 2017-03-17 2022-10-31 Chr Hansen Hmo Gmbh Fremgangsmåde til hæmning af isomerisering af et reducerende saccharid efter termisk behandling
EP3425052A1 (en) * 2017-07-07 2019-01-09 Jennewein Biotechnologie GmbH Fucosyltransferases and their use in producing fucosylated oligosaccharides
EP3438122A1 (en) * 2017-08-01 2019-02-06 OligoScience Biotechnology GmbH Microorganism for producing human milk oligosaccharide
KR101953375B1 (ko) * 2017-11-20 2019-02-28 고려대학교 산학협력단 다양하고 신규한 퓨코실 올리고당의 제조법 및 이의 용도
CN109402020B (zh) * 2018-11-30 2020-08-04 江南大学 一株高产精氨酸氨肽酶的瑞士乳杆菌及其应用
CN109735479B (zh) * 2019-01-30 2022-04-01 光明乳业股份有限公司 一种合成2’-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法与应用
RS65200B1 (sr) * 2019-03-04 2024-03-29 Aurealis Therapeutics AG Prokariotski ekspresioni sistem inducibilan hloridom
WO2020246585A1 (ja) 2019-06-05 2020-12-10 森永乳業株式会社 栄養組成物
JP2023506287A (ja) * 2019-12-17 2023-02-15 インバイオス エン．フェー． ラクトースを変換するアルファ－１，２－フコシルトランスフェラーゼ酵素
CN111218488B (zh) * 2020-02-10 2022-11-15 中国科学院上海高等研究院 一种利用大肠杆菌生产2’-岩藻糖基乳糖的方法
CN111471606B (zh) * 2020-03-17 2022-03-08 山东大学 一株优化的高产岩藻糖基乳糖的酿酒酵母菌株及其应用
CN111471605B (zh) * 2020-03-17 2022-03-08 山东大学 一株高产岩藻糖基乳糖的酿酒酵母工程菌株及其应用
KR102719035B1 (ko) * 2020-04-20 2024-10-18 주식회사 리비옴 혈관 작동성 장 펩티드를 발현하는 미생물, 및 그의 용도
EP3954769A1 (en) * 2020-08-10 2022-02-16 Inbiose N.V. Production of oligosaccharide mixtures by a cell
US11331329B2 (en) 2020-05-13 2022-05-17 Glycosyn LLC Fucosylated oligosaccharides for prevention of coronavirus infection
CA3178736A1 (en) 2020-05-13 2021-11-18 Ardythe L. Morrow 2'-fucosyllactose for the prevention and treatment of coronavirus-induced inflammation
CN113969256A (zh) * 2020-07-24 2022-01-25 上海交通大学 一种生产n-乙酰氨基葡萄糖的菌株及其构建方法和应用
EP4192944A1 (en) 2020-08-10 2023-06-14 Inbiose N.V. Production of oligosaccharide mixtures by a cell
EP4200318A4 (en) * 2020-08-19 2024-06-12 Conagen Inc. BIOSYNTHETIC PRODUCTION OF 2-FUCOSYLLACTOSE
KR102793833B1 (ko) * 2020-12-31 2025-04-11 주식회사 삼양사 푸코오스 전이효소를 발현하는 재조합 미생물 및 이를 이용한 2’-푸코실락토오스 제조방법
KR102633804B1 (ko) * 2020-12-31 2024-02-05 주식회사 삼양사 재조합 바실러스 속 미생물 및 이를 이용한 모유올리고당 제조방법
WO2023182970A1 (en) * 2021-02-19 2023-09-28 The Regents Of The University Of California Methods for production of fucosylated oligosaccharides in recombinant cell culture
WO2022176994A1 (ja) * 2021-02-19 2022-08-25 協和発酵バイオ株式会社 改変されたα１，２－フコシルトランスフェラーゼ活性を有する蛋白質及びフコース含有糖質の製造法
DK181242B1 (en) 2021-05-17 2023-05-30 Dsm Ip Assets Bv GENETICALLY ENGINEERED CELLS COMPRISING A RECOMBINANT NUCLEIC ACID SEQUNCE ENCODING AN α-1,2-FUCOSYLTRANSFERASE CAPABLE OF PRODUCING LNFP-I, NUCLEIC ACID SEQUENCES ENCODING SAME AND METHODS FOR USE OF SAME
EP4370664A1 (en) * 2021-07-16 2024-05-22 Inbiose N.V. Lacto-n-biose converting fucosyltransferases
CN113817701B (zh) * 2021-09-30 2023-08-18 中南大学 α-1,2-岩藻糖基转移酶突变体及其应用
CN113832092B (zh) * 2021-10-20 2023-10-03 江南大学 一种提高乳酰-n-岩藻五糖产量的基因工程菌及其生产方法
CN116286919A (zh) * 2021-12-03 2023-06-23 虹摹生物科技(上海)有限公司 基因工程菌及利用其制备岩藻糖基化寡糖的方法
KR20240017427A (ko) * 2022-07-29 2024-02-08 주식회사 진켐 3-푸코실락토스 생산용 유전자 조작 미생물 및 이를 이용한 방법
CN116042671B (zh) * 2023-02-14 2024-11-22 华熙生物科技股份有限公司 一种用于制备母乳寡糖的核酸分子、表达载体和微生物
CN116769808B (zh) * 2023-02-27 2025-06-10 江南大学 一种专一生产2′-岩藻糖基乳糖的菌株及应用
WO2025087087A1 (zh) * 2023-10-23 2025-05-01 山东恒鲁生物科技有限公司 一种岩藻糖基转移酶多肽及其应用
CN119614406B (zh) * 2023-10-23 2025-09-19 恒睿（青岛）生物科技有限公司 一种克鲁维酵母基因修饰细胞

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US20140031541A1 (en) * 2012-07-25 2014-01-30 Glycosyn LLC Alpha (1,2) Fucosyltransferases Suitable for Use in the Production of Fucosylated Oligosaccharides
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US20140031541A1 (en) * 2012-07-25 2014-01-30 Glycosyn LLC Alpha (1,2) Fucosyltransferases Suitable for Use in the Production of Fucosylated Oligosaccharides
WO2015150328A1 (en) * 2014-03-31 2015-10-08 Jennewein Biotechnologie Gmbh Total fermentation of oligosaccharides

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US20220056497A1 (en) 2022-02-24
JP2022173457A (ja) 2022-11-18
EP4293033A2 (en) 2023-12-20
EP4293033A3 (en) 2024-04-10
ES2962258T3 (es) 2024-03-18
SG11201609366TA (en) 2016-12-29
MX2016014807A (es) 2017-05-10
EP3083938C0 (en) 2023-11-08
CN106795484A (zh) 2017-05-31
EP3083938A1 (en) 2016-10-26
KR20230113650A (ko) 2023-07-31
SG10202010599TA (en) 2020-11-27
CA2945661A1 (en) 2015-11-19
KR20170035835A (ko) 2017-03-31
EP3083938A4 (en) 2017-05-31
JP2017515455A (ja) 2017-06-15
WO2015175801A9 (en) 2016-10-20
EP3083938B1 (en) 2023-11-08
AU2015259088A2 (en) 2018-06-28
JP2020188761A (ja) 2020-11-26
US11643675B2 (en) 2023-05-09
NZ722185A (en) 2023-12-22
AU2015259088A1 (en) 2016-08-04
US11046984B2 (en) 2021-06-29
US20170081353A1 (en) 2017-03-23
WO2015175801A1 (en) 2015-11-19
HUE064860T2 (hu) 2024-04-28
CN106795484B (zh) 2022-09-09
CA2945661C (en) 2023-10-17

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