CA2912553C - Method for production of botulinum toxin - Google Patents

Method for production of botulinum toxin Download PDF

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Publication number
CA2912553C
CA2912553C CA2912553A CA2912553A CA2912553C CA 2912553 C CA2912553 C CA 2912553C CA 2912553 A CA2912553 A CA 2912553A CA 2912553 A CA2912553 A CA 2912553A CA 2912553 C CA2912553 C CA 2912553C
Authority
CA
Canada
Prior art keywords
botulinum toxin
botulinum
toxin
precipitate
anion exchange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2912553A
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English (en)
French (fr)
Other versions
CA2912553A1 (en
Inventor
Chung Sei Kim
Kwan Young Song
Kyoung Min Min
Yeong Duk An
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daewoong Co Ltd
Original Assignee
Daewoong Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49987856&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2912553(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Daewoong Co Ltd filed Critical Daewoong Co Ltd
Publication of CA2912553A1 publication Critical patent/CA2912553A1/en
Application granted granted Critical
Publication of CA2912553C publication Critical patent/CA2912553C/en
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2912553A 2013-08-02 2014-05-07 Method for production of botulinum toxin Active CA2912553C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020130092024A KR101339349B1 (ko) 2013-08-02 2013-08-02 보툴리눔 독소의 제조방법
KR10-2013-0092024 2013-08-02
PCT/KR2014/004003 WO2015016462A1 (en) 2013-08-02 2014-05-07 Method for production of botulinum toxin

Publications (2)

Publication Number Publication Date
CA2912553A1 CA2912553A1 (en) 2015-02-05
CA2912553C true CA2912553C (en) 2018-01-02

Family

ID=49987856

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2912553A Active CA2912553C (en) 2013-08-02 2014-05-07 Method for production of botulinum toxin

Country Status (25)

Country Link
US (1) US9512418B2 (en:Method)
EP (1) EP2920199B1 (en:Method)
JP (2) JP6307150B2 (en:Method)
KR (1) KR101339349B1 (en:Method)
CN (1) CN105392795B (en:Method)
AR (1) AR096541A1 (en:Method)
AU (1) AU2014297167B2 (en:Method)
BR (1) BR112015029973B1 (en:Method)
CA (1) CA2912553C (en:Method)
CY (1) CY1120410T1 (en:Method)
DK (1) DK2920199T3 (en:Method)
ES (1) ES2665288T3 (en:Method)
HR (1) HRP20180679T1 (en:Method)
HU (1) HUE036840T2 (en:Method)
LT (1) LT2920199T (en:Method)
MX (1) MX362821B (en:Method)
NO (1) NO2920199T3 (en:Method)
PH (1) PH12015501855A1 (en:Method)
PL (1) PL2920199T3 (en:Method)
PT (1) PT2920199T (en:Method)
RU (1) RU2627159C2 (en:Method)
SA (1) SA515370208B1 (en:Method)
SG (1) SG11201509486VA (en:Method)
SI (1) SI2920199T1 (en:Method)
WO (1) WO2015016462A1 (en:Method)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8129139B2 (en) 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
KR101339349B1 (ko) * 2013-08-02 2013-12-09 주식회사 대웅 보툴리눔 독소의 제조방법
KR101723167B1 (ko) 2015-04-28 2017-04-05 주식회사 대웅 보툴리눔 독소의 제조를 위한 배지 조성물
KR101729251B1 (ko) 2015-04-28 2017-04-21 주식회사 대웅 보툴리눔 독소의 제조를 위한 배지 조성물
KR101723168B1 (ko) 2015-04-28 2017-04-05 주식회사 대웅 보툴리눔 독소의 제조를 위한 배지 조성물
KR101853463B1 (ko) * 2015-09-09 2018-06-20 휴젤(주) 보툴리눔 독소의 정제 방법
KR101775682B1 (ko) 2015-11-30 2017-09-06 주식회사 대웅 보툴리눔 독소의 제조방법
CN109641941B (zh) * 2016-05-16 2023-06-16 哈佛大学校长及研究员协会 用于纯化和激活肉毒杆菌神经毒素的方法
KR102463881B1 (ko) 2016-10-04 2022-11-07 (주)메디톡스 보툴리눔 독소 함유 용액으로부터 보툴리눔 독소를 분리하는 방법
KR101744900B1 (ko) 2017-01-20 2017-06-08 주식회사 대웅 보툴리눔 독소를 포함하는 안정한 액상 조성물
CN109646673A (zh) * 2017-10-12 2019-04-19 秀杰股份公司 肉毒杆菌毒素的微结构制剂技术
CA3124535A1 (en) 2018-12-21 2020-06-25 The Research Foundation For Microbial Diseases Of Osaka University Botulinum toxin producing method
KR102516204B1 (ko) * 2019-04-15 2023-03-30 (주)제테마 보툴리눔 독소의 정제방법
KR102516203B1 (ko) * 2019-04-15 2023-03-30 (주)제테마 보툴리눔 독소의 제조방법
TWI854077B (zh) * 2019-12-20 2024-09-01 瑞士商葛德瑪控股公司 生產肉毒桿菌毒素的方法
TW202136286A (zh) * 2019-12-20 2021-10-01 瑞士商葛德瑪控股公司 純化肉毒桿菌毒素的方法
KR102724651B1 (ko) * 2021-07-08 2024-11-01 주식회사 파마리서치바이오 비-독소 단백질이 제거된 클로스트리디움 보툴리눔 신경독소 단백질의 정제방법
TWI852454B (zh) * 2022-04-01 2024-08-11 大陸商重慶譽顏製藥有限公司 一種肉毒毒素蛋白組合物、製備方法及其應用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030060150A (ko) * 2002-01-07 2003-07-16 (주)메디톡스 클로스트리디움 보툴리눔 ａ형 독소를 정제하는 방법
RU2206337C1 (ru) * 2002-10-29 2003-06-20 Общество с ограниченной ответственностью "Токсины и сопутствующие продукты" Лекарственный препарат для лечения мышечных дистоний и способ его получения
US7452697B2 (en) * 2003-09-25 2008-11-18 Allergan, Inc. Chromatographic method and system for purifying a botulinum toxin
US7879341B2 (en) * 2004-07-26 2011-02-01 Merz Pharma Gmbh & Co. Kgaa Therapeutic composition with a botulinum neurotoxin
AU2005327458B2 (en) * 2005-03-03 2011-12-15 Allergan, Inc. Animal product free system and process for purifying a botulinum toxin
KR101025617B1 (ko) * 2008-02-25 2011-03-30 메덱스젠 주식회사 클로스트리디움 보툴리눔으로부터 보툴리눔 독소를정제하는 방법
US8129139B2 (en) * 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
HRP20181869T1 (hr) * 2009-10-21 2019-01-11 Revance Therapeutics, Inc. Metode i sustavi za pročišćavanje nekompleksiranog botulinskog neurotoksina
KR101434558B1 (ko) * 2011-07-21 2014-08-27 (주)메디톡스 식물 유래 성분 함유 배지 및 가요성 폐쇄 용기를 이용하여 클로스트리디움 보툴리눔 독소를 생산하는 방법
KR101339349B1 (ko) * 2013-08-02 2013-12-09 주식회사 대웅 보툴리눔 독소의 제조방법

Also Published As

Publication number Publication date
US20150337281A1 (en) 2015-11-26
ES2665288T3 (es) 2018-04-25
CN105392795A (zh) 2016-03-09
PL2920199T3 (pl) 2018-08-31
EP2920199B1 (en) 2018-03-07
KR101339349B1 (ko) 2013-12-09
JP2017141237A (ja) 2017-08-17
MX362821B (es) 2019-02-18
RU2015151186A (ru) 2017-06-01
PT2920199T (pt) 2018-05-30
HUE036840T2 (hu) 2018-08-28
WO2015016462A1 (en) 2015-02-05
HRP20180679T1 (hr) 2018-06-01
SI2920199T1 (en) 2018-06-29
CN105392795B (zh) 2019-11-22
CA2912553A1 (en) 2015-02-05
AU2014297167A1 (en) 2015-12-10
BR112015029973A2 (pt) 2017-07-25
BR112015029973B1 (pt) 2023-01-17
CY1120410T1 (el) 2019-07-10
AU2014297167B2 (en) 2016-11-17
RU2627159C2 (ru) 2017-08-03
JP6307150B2 (ja) 2018-04-04
SA515370208B1 (ar) 2017-07-08
LT2920199T (lt) 2018-04-10
US9512418B2 (en) 2016-12-06
HK1215715A1 (zh) 2016-09-09
EP2920199A1 (en) 2015-09-23
PH12015501855B1 (en) 2015-12-07
PH12015501855A1 (en) 2015-12-07
JP2016521968A (ja) 2016-07-28
DK2920199T3 (en) 2018-05-28
AR096541A1 (es) 2016-01-13
MX2015016481A (es) 2016-06-10
NO2920199T3 (en:Method) 2018-08-04
SG11201509486VA (en) 2015-12-30
EP2920199A4 (en) 2016-10-05

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