CA2867665C - Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing - Google Patents

Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing Download PDF

Info

Publication number
CA2867665C
CA2867665C CA2867665A CA2867665A CA2867665C CA 2867665 C CA2867665 C CA 2867665C CA 2867665 A CA2867665 A CA 2867665A CA 2867665 A CA2867665 A CA 2867665A CA 2867665 C CA2867665 C CA 2867665C
Authority
CA
Canada
Prior art keywords
flow cell
read head
nucleic acid
cartridge
acid sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2867665A
Other languages
English (en)
French (fr)
Other versions
CA2867665A1 (en
Inventor
Dale Buermann
John A. Moon
Bryan Crane
Mark Wang
Stanley S. Hong
Jason Harris
Matthew Hage
Mark J. Nibbe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Priority to CA3138752A priority Critical patent/CA3138752C/en
Publication of CA2867665A1 publication Critical patent/CA2867665A1/en
Application granted granted Critical
Publication of CA2867665C publication Critical patent/CA2867665C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G01N2021/0307—Insert part in cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0325—Cells for testing reactions, e.g. containing reagents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G01N2021/058—Flat flow cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Optics & Photonics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Saccharide Compounds (AREA)
  • Catalysts (AREA)
CA2867665A 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing Active CA2867665C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3138752A CA3138752C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261619784P 2012-04-03 2012-04-03
US61/619,784 2012-04-03
PCT/US2013/025963 WO2013151622A1 (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3138752A Division CA3138752C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Publications (2)

Publication Number Publication Date
CA2867665A1 CA2867665A1 (en) 2013-10-10
CA2867665C true CA2867665C (en) 2022-01-04

Family

ID=47891920

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2867665A Active CA2867665C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
CA3138752A Active CA3138752C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3138752A Active CA3138752C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Country Status (13)

Country Link
US (5) US9193996B2 (https=)
EP (2) EP2834622B1 (https=)
JP (1) JP6159391B2 (https=)
KR (3) KR20210080618A (https=)
CN (1) CN204832037U (https=)
AU (4) AU2013243998B2 (https=)
BR (2) BR122021002358B8 (https=)
CA (2) CA2867665C (https=)
ES (1) ES2949570T3 (https=)
IL (3) IL234864B (https=)
IN (1) IN2014DN07992A (https=)
MX (1) MX337140B (https=)
WO (1) WO2013151622A1 (https=)

Families Citing this family (347)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HRP20151294T1 (hr) 2010-04-05 2016-03-11 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2834622B1 (en) * 2012-04-03 2023-04-12 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US8932815B2 (en) 2012-04-16 2015-01-13 Biological Dynamics, Inc. Nucleic acid sample preparation
BR112014025695B1 (pt) 2012-04-16 2021-08-03 Biological Dynamics, Inc Preparação de amostra de ácido nucleico
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
WO2015021228A1 (en) * 2013-08-08 2015-02-12 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
CN206281782U (zh) 2013-08-28 2017-06-27 伊鲁米那股份有限公司 检查设备及光学检测装置
US10540783B2 (en) * 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
RS60736B1 (sr) 2013-12-03 2020-09-30 Illumina Inc Postupci i sistemi za analizu podataka sa slika
ES2866044T3 (es) 2014-02-18 2021-10-19 Illumina Inc Métodos y composiciones para la elaboración de perfiles de ADN
CN106459869B (zh) * 2014-03-11 2020-01-21 伊鲁米那股份有限公司 一次性的集成微流体盒及其制备和使用的方法
US10570447B2 (en) 2014-05-16 2020-02-25 Illumina, Inc. Nucleic acid synthesis techniques
CN110038653A (zh) * 2014-05-27 2019-07-23 伊鲁米那股份有限公司 包括基本仪器和可拆卸盒的用于生物化学分析的系统和方法
RU2688746C2 (ru) * 2014-06-05 2019-05-22 Иллумина, Инк. Системы и способы с использованием поворотного клапана для по меньшей мере одного из приготовления образца или анализа образца
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
GB201416422D0 (en) * 2014-09-17 2014-10-29 Illumina Cambridge Ltd Flexible tape-based chemistry apparatus
US10118173B2 (en) 2014-10-09 2018-11-06 Illumina, Inc. Method and device for separating immiscible liquids to effectively isolate at least one of the liquids
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
EP3215616B1 (en) 2014-11-05 2019-12-18 Illumina Cambridge Limited Reducing dna damage during sample preparation and sequencing using siderophore chelators
DK3218511T3 (da) 2014-11-11 2020-07-20 Illumina Cambridge Ltd Fremgangsmåder og arrays til frembringelse og sekventering af monoklonale klynger af nukleinsyre
WO2016130964A1 (en) 2015-02-13 2016-08-18 Abbott Laboratories Decapping and capping apparatus, systems and methods for use in diagnostic analyzers
USD766761S1 (en) * 2015-03-20 2016-09-20 Life Technologies Corporation Analytical equipment
WO2016154038A1 (en) 2015-03-20 2016-09-29 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
EP4089398B1 (en) 2015-03-24 2024-09-04 Illumina, Inc. Carrier assemblies and systems for imaging samples for biological or chemical analysis
CN107532205B (zh) 2015-03-31 2021-06-08 伊卢米纳剑桥有限公司 模板的表面连环化
US10379046B2 (en) * 2015-04-08 2019-08-13 Molecular Devices, Llc Method and system for multiplexed time-resolved fluorescence detection
US11971354B2 (en) 2015-04-08 2024-04-30 Molecular Devices, Llc Methods and systems for fluorescence detection using infrared dyes
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US9222935B1 (en) * 2015-05-28 2015-12-29 Pixcell Medical Technologies Ltd Fluid sample analysis system
WO2016196358A1 (en) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Methods of analyzing nucleic acids
ES2789349T3 (es) 2015-05-29 2020-10-26 Illumina Cambridge Ltd Utilización mejorada de cebadores de superficie en grupos
CN107922966B (zh) 2015-07-06 2022-06-28 伊卢米纳剑桥有限公司 用于核酸扩增的样品制备
ES2888626T3 (es) 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
SG10202106413SA (en) 2015-08-14 2021-07-29 Illumina Inc Systems and methods using magnetically-responsive sensors for determining a genetic characteristic
EP3338096B1 (en) 2015-08-24 2024-03-06 Illumina, Inc. In-line pressure accumulator and flow-control system for biological or chemical assays
JP6720526B2 (ja) * 2015-12-25 2020-07-08 東洋紡株式会社 検査装置
KR20180108578A (ko) * 2016-01-11 2018-10-04 일루미나, 인코포레이티드 마이크로 형광 측정기, 유체 시스템, 및 플로우 셀 래치 클램프 모듈을 구비하는 검출 장치
USD819829S1 (en) * 2016-02-12 2018-06-05 Illumina, Inc. Sequencing cartridge
US10508305B2 (en) * 2016-02-28 2019-12-17 Damoun Nashtaali DNA sequencing and processing
US10232369B2 (en) 2016-03-24 2019-03-19 Biological Dynamics, Inc. Disposable fluidic cartridge and components
US20170274374A1 (en) 2016-03-28 2017-09-28 Ilumina, Inc. Multi-plane microarrays
ITUA20162851A1 (it) * 2016-04-04 2017-10-04 Euritmi S A S Di Dalla Sega Daniela E C Sistema per microscopia
CN117822128A (zh) 2016-07-22 2024-04-05 俄勒冈健康与科学大学 单细胞全基因组文库及制备其的组合索引方法
EP3493908B1 (en) 2016-08-05 2023-11-29 Vital Signs Solutions Limited Device and method for liquid analysis to detect biomarkers
AU2017330438A1 (en) * 2016-09-23 2019-05-16 ArcherDX, Inc. Fluidic system and related methods
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
MY194951A (en) 2016-10-14 2022-12-28 Illumina Inc Cartridge assembly
USD825078S1 (en) * 2016-10-14 2018-08-07 Illumina, Inc. Sequencing cartridge
CN108265112B (zh) 2016-12-30 2023-04-25 深圳市真迈生物科技有限公司 对序列测定反应进行控制的方法、序列测定系统和控制装置
GB201704763D0 (https=) * 2017-01-05 2017-05-10 Illumina Inc
GB201704772D0 (en) 2017-01-05 2017-05-10 Illumina Inc Automated volumetric reagent delivery testing
GB201704768D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Flow cell liquid degassing systema and method
GB201704747D0 (en) 2017-01-05 2017-05-10 Illumina Inc Reagent mixing system and methods
GB201704761D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Common line selector valve for a system
GB201704766D0 (en) * 2017-01-05 2017-05-10 Illumia Inc System and methods for selective effluent collection
GB201704760D0 (en) 2017-01-05 2017-05-10 Illumina Inc Reagent nozzle sipper mixing system and method
CN116612818A (zh) 2017-01-06 2023-08-18 伊鲁米那股份有限公司 移相校正
TWI772752B (zh) 2017-01-07 2022-08-01 美商伊路米納有限公司 光學偵測裝置以及方法
WO2018136416A1 (en) 2017-01-17 2018-07-26 Illumina, Inc. Oncogenic splice variant determination
GB201701688D0 (en) * 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
GB201701691D0 (en) 2017-02-01 2017-03-15 Illumina Inc System and method with reflective fiducials
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
US11896944B2 (en) 2017-02-01 2024-02-13 Illumina, Inc. System and method with fiducials responding to multiple excitation frequencies
BR112019014683A2 (pt) * 2017-03-07 2020-02-18 Illumina, Inc. Sequenciamento com dois canais óticos, com fonte de luz única
CA3057326A1 (en) * 2017-03-22 2018-09-27 The Board Of Trustees Of The University Of Illinois System for rapid, portable, and multiplexed detection and identification of pathogen specific nucleic acid sequences
WO2018183745A1 (en) 2017-03-30 2018-10-04 Illumina, Inc. Genomic data analysis system and method
US10737267B2 (en) 2017-04-04 2020-08-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
EP3615671B1 (en) 2017-04-23 2021-07-21 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3622084A4 (en) 2017-05-08 2021-02-17 Biological Dynamics, Inc. METHODS AND SYSTEMS FOR PROCESSING ANALYTICAL INFORMATION
FI3981884T3 (fi) 2017-06-07 2023-09-21 Univ Oregon Health & Science Yksittäissolujen kokogenomikirjastoja metylaatiosekvensointia varten
EP3642362B1 (en) 2017-06-20 2025-10-15 Illumina, Inc. Methods for addressing inefficiencies in amplification reactions
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. SEQUENCING SYSTEM COMPRISING AGGREGATION OF MULTIPLEXED BIOLOGICAL SAMPLES
FI3662083T3 (fi) 2017-08-01 2024-11-20 Illumina Inc Geneettisen materiaalin ja kirjastovalmisteen spatiaalinen indeksointi hydrogeelihelmiä ja virtauskennoja käyttämällä
WO2019035897A1 (en) 2017-08-15 2019-02-21 Omniome, Inc. SCANNING APPARATUS AND METHODS USEFUL FOR DETECTION OF CHEMICAL OR BIOLOGICAL ANALYTES
CN111566261B (zh) 2017-08-18 2025-03-25 诺迪勒思附属公司 选择结合试剂的方法
WO2019116296A1 (en) * 2017-12-15 2019-06-20 Creoptix Ltd. Methods and assemblies for high throughput screening
WO2019118989A1 (en) 2017-12-15 2019-06-20 Evanostics Llc Optical reader for analyte testing
WO2019126388A1 (en) 2017-12-19 2019-06-27 Biological Dynamics, Inc. Methods and devices for detection of multiple analytes from a biological sample
KR20250152680A (ko) 2017-12-29 2025-10-23 노틸러스 서브시디어리, 인크. 단백질 식별을 위한 디코딩 접근법
US12188012B2 (en) 2018-04-02 2025-01-07 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
WO2019195196A1 (en) 2018-04-02 2019-10-10 Biological Dynamics, Inc. Dielectric materials
US11441978B1 (en) * 2018-04-12 2022-09-13 Elemental Scientific, Inc. Automatic evaporative sample preparation
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
WO2019221913A1 (en) 2018-05-15 2019-11-21 Illumina, Inc. Flow cell with flexible connection
NL2021147B1 (en) * 2018-05-15 2019-11-21 Illumina Inc Flow cell with flexible connection
IL271454B2 (en) 2018-05-17 2025-04-01 Illumina Inc Rapid single-cell genetic sequencing with low Aggregation bias
CN111247248A (zh) 2018-06-04 2020-06-05 伊鲁米纳公司 高通量单细胞转录组文库及制备和使用方法
JP6688342B2 (ja) * 2018-07-06 2020-04-28 日本板硝子株式会社 反応処理装置
DE102018212489A1 (de) 2018-07-26 2020-01-30 Implen GmbH Vorrichtung für eine lichtspektroskopische Analyse
EP3844304B1 (en) 2018-08-28 2024-10-02 10X Genomics, Inc. Methods for generating spatially barcoded arrays
EP3844308A1 (en) 2018-08-28 2021-07-07 10X Genomics, Inc. Resolving spatial arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
NL2021970B1 (en) * 2018-10-05 2020-05-12 Illumina Inc System and method for sequestered wash buffered reuse
US11426723B2 (en) * 2018-10-05 2022-08-30 Illumina, Inc. System and method for sequestered wash buffered reuse
JP2022512732A (ja) 2018-10-18 2022-02-07 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 流体サンプル中の運動性オブジェクトの運動性ベースのラベルフリー検出のための装置および方法
US10768173B1 (en) * 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
EP3884048A4 (en) 2018-11-20 2022-08-17 Nautilus Biotechnology, Inc. DESIGN AND SELECTION OF AFFINITY REAGENTS
CN111277744B (zh) * 2018-12-04 2022-04-15 武汉华大智造科技有限公司 对焦方法及系统、基因测序仪、计算机可读存储介质
CN211311477U (zh) 2018-12-04 2020-08-21 欧姆尼欧美公司 测序系统
WO2020118255A1 (en) * 2018-12-07 2020-06-11 Element Biosciences, Inc. Flow cell device and use thereof
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894590B1 (en) 2018-12-10 2025-10-22 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
EP3894835A1 (en) * 2018-12-11 2021-10-20 Carrier Corporation Calibration of an optical detector using a micro-flow chamber
JP7542444B2 (ja) * 2018-12-14 2024-08-30 イルミナ ケンブリッジ リミテッド 配列決定の間のアンラベル化ヌクレオチドによるフェージング減少
WO2020132146A1 (en) 2018-12-18 2020-06-25 Evanostics Llc Optical analyte detection
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11493441B2 (en) 2019-01-15 2022-11-08 Wyatt Technology Corporation Flow cell, read head, and skid attachment
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
WO2020172444A1 (en) 2019-02-20 2020-08-27 Omniome, Inc. Scanning apparatus and methods for detecting chemical and biological analytes
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
CA3113841A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
WO2020190509A1 (en) 2019-03-15 2020-09-24 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
WO2020191390A2 (en) 2019-03-21 2020-09-24 Illumina, Inc. Artificial intelligence-based quality scoring
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
CA3135206A1 (en) 2019-04-29 2020-11-05 Pierre Indermuhle Methods and systems for integrated on-chip single-molecule detection
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2020252186A1 (en) 2019-06-11 2020-12-17 Omniome, Inc. Calibrated focus sensing
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
EP4028407B1 (en) 2019-09-10 2023-08-16 Pacific Biosciences of California, Inc. Reversible modification of nucleotides
CA3123094A1 (en) 2019-09-18 2021-03-25 Illumina, Inc. Systems and related sample loading manifold assemblies
JP7618558B2 (ja) * 2019-09-18 2025-01-21 イルミナ インコーポレイテッド システム及び関連するポンプマニホールドアセンブリ
US12117396B2 (en) 2019-09-24 2024-10-15 Mgi Tech Co., Ltd. Biochemical substance analysis system, method, and device
EP4038546B1 (en) 2019-10-01 2024-08-21 10X Genomics, Inc. Systems and methods for identifying morphological patterns in tissue samples
AU2020372998A1 (en) * 2019-10-29 2022-06-02 Quantum-Si Incorporated Peristaltic pumping of fluids for bioanalytical applications and associated methods, systems, and devices
AU2020372908A1 (en) 2019-10-29 2022-06-02 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
AU2020388047A1 (en) 2019-11-18 2022-06-23 10X Genomics, Inc. Systems and methods for tissue classification
WO2021102039A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc, Spatial analysis of analytes
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
EP3927824A2 (en) 2019-12-19 2021-12-29 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
EP4084904A4 (en) * 2019-12-30 2024-05-29 Illumina, Inc. FLOW CELL ARRANGEMENTS AND RELATED REAGENT SELECTION VALVES
MX2021016073A (es) * 2019-12-31 2022-03-25 Illumina Inc Funcionalidad de enfoque automático en el análisis óptico de muestras.
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11408032B2 (en) * 2020-01-17 2022-08-09 Element Biosciences, Inc. Tube lens design for improved depth-of-field
JP7233385B2 (ja) * 2020-01-20 2023-03-06 日本板硝子株式会社 反応処理装置
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
WO2021158511A1 (en) 2020-02-04 2021-08-12 Omniome, Inc. Flow cells and methods for their manufacture and use
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
EP4107284A1 (en) 2020-02-17 2022-12-28 10X Genomics, Inc. In situ analysis of chromatin interaction
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
EP4484571A3 (en) 2020-02-21 2025-03-19 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
KR20230035237A (ko) 2020-05-12 2023-03-13 일루미나, 인코포레이티드 재조합 말단 데옥시뉴클레오티딜 트랜스퍼라제를 사용하여 변형된 염기를 갖는 핵산의 생성
CN116134308B (zh) 2020-05-19 2025-05-27 10X基因组学有限公司 电泳盒和仪器
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
AU2021288692A1 (en) 2020-06-11 2023-02-02 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
AU2021299216A1 (en) 2020-06-30 2022-12-08 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless DNA
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
KR20230038145A (ko) 2020-07-08 2023-03-17 일루미나, 인코포레이티드 트랜스포좀 담체로서의 비드
US12580044B1 (en) 2020-09-02 2026-03-17 10X Genomics, Inc. Systems and methods for identifying cells that are antigen-specific for an immunogenic feature
EP4491742B1 (en) 2020-09-18 2026-03-04 10X Genomics, Inc. Sample handling apparatus and image registration methods
CN114682310B (zh) * 2020-12-31 2023-12-05 深圳市真迈生物科技有限公司 液路系统、测序系统和方法
EP4224020A4 (en) * 2020-09-29 2024-11-13 GeneMind Biosciences Company Limited FLOW PATH SELECTION VALUE, SYSTEM AND METHOD, STORAGE MEDIUM AND APPLICATION
CN114688301A (zh) * 2020-12-31 2022-07-01 深圳市真迈生物科技有限公司 流动路径选择阀、流动池组件、液路系统及测序系统
CN114321036A (zh) * 2020-09-29 2022-04-12 深圳市真迈生物科技有限公司 液路系统、检测系统和应用
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
CA3196729A1 (en) 2020-11-11 2022-05-19 Tural AKSEL Affinity reagents having enhanced binding and detection characteristics
GB2603451B (en) * 2020-11-25 2024-10-02 Oxford Nanoimaging Ltd Reagent cartridge and measurement devices incorporating such cartridges
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
JP2024501848A (ja) 2020-12-31 2024-01-16 キュー－ステート バイオサイエンシーズ， インコーポレイテッド 光遺伝学のためのイメージャの較正および正規化
JP2024501850A (ja) 2020-12-31 2024-01-16 キュー－ステート バイオサイエンシーズ， インコーポレイテッド 空間撮像およびビームホモジナイザを伴う顕微鏡
US12478971B2 (en) 2020-12-31 2025-11-25 Quiver Holdings Inc. Optical plate controller and reader
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4281775B1 (en) 2021-01-21 2026-04-29 Nautilus Subsidiary, Inc. Systems and methods for biomolecule preparation
EP4284945B1 (en) 2021-01-26 2024-10-23 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
WO2022187366A1 (en) 2021-03-03 2022-09-09 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
US20230356230A1 (en) * 2021-03-03 2023-11-09 Illumina, Inc. Reagent cartridges and related systems and methods for controlling reagent temperature
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
CN117043867B (zh) 2021-04-02 2025-11-28 因美纳有限公司 用于检测用于测序的核苷酸样品玻片内的气泡的机器学习模型
US20220380838A1 (en) 2021-06-01 2022-12-01 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
WO2022256422A1 (en) 2021-06-02 2022-12-08 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
US20220403450A1 (en) 2021-06-03 2022-12-22 Illumina Software, Inc. Systems and methods for sequencing nucleotides using two optical channels
EP4359555A1 (en) 2021-06-23 2024-05-01 Illumina, Inc. Compositions, methods, kits, cartridges, and systems for sequencing reagents
WO2022269033A1 (en) 2021-06-25 2022-12-29 Illumina Cambridge Limited Linear fourier fiducial
WO2022271689A1 (en) 2021-06-25 2022-12-29 Illumina, Inc. Fiducials for use in registration of a patterned surface
WO2023278966A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Machine-learning model for generating confidence classifications for genomic coordinates
IL309308A (en) 2021-06-29 2024-02-01 Illumina Inc A signal-to-noise ratio measure for determining nucleotide base reads and base call quality
EP4363832A4 (en) 2021-06-30 2025-05-07 Q-State Biosciences, Inc. MULTIPLEX OPTICAL STIMULUS AND READOUT PROCESS
CN117651855A (zh) 2021-07-13 2024-03-05 10X基因组学有限公司 用于制备具有可控厚度的聚合基质的方法
KR20240031968A (ko) 2021-07-19 2024-03-08 일루미나, 인코포레이티드 염기 호출에 대한 보간 및 적응을 갖는 강도 추출
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
KR20240063107A (ko) 2021-07-21 2024-05-10 엘리먼트 바이오사이언스, 인크. 핵산 시퀀싱을 위한 광학 시스템 및 이의 방법
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
CA3224093A1 (en) 2021-07-30 2023-02-02 Jorge Ivan Hernandez Neuta Methods and compositions for synchronizing reactions in situ
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
EP4381095A1 (en) 2021-08-03 2024-06-12 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US20230051226A1 (en) 2021-08-10 2023-02-16 Mgi Tech Co., Ltd. Flow cell imaging systems and methods, and flow cells and other substrates for use in the same
US12435364B2 (en) 2021-08-16 2025-10-07 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
EP4344443A1 (en) 2021-08-31 2024-04-03 Illumina, Inc. Flow cell with enhanced well imaging resolution
JP2024534741A (ja) 2021-09-01 2024-09-26 イルミナ インコーポレイテッド 加速した塩基呼び出しのための振幅変調
CA3227872A1 (en) 2021-09-09 2023-03-16 James Henry JOLY Characterization and localization of protein modifications
JP2024535663A (ja) 2021-09-17 2024-10-02 イルミナ インコーポレイテッド ヌクレオチド配列決定における塩基コールエラーパターンからの障害ソースの自動的特定
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
US20230095961A1 (en) 2021-09-21 2023-03-30 Illumina, Inc. Graph reference genome and base-calling approach using imputed haplotypes
WO2023049215A1 (en) 2021-09-22 2023-03-30 Illumina, Inc. Compressed state-based base calling
CA3227592A1 (en) 2021-09-22 2023-03-30 Gregory KAPP Methods and systems for determining polypeptide interactions
AU2022367166A1 (en) 2021-10-11 2024-04-04 Nautilus Subsidiary, Inc. Highly multiplexable analysis of proteins and proteomes
US20230149883A1 (en) 2021-11-03 2023-05-18 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
US20230343415A1 (en) 2021-12-02 2023-10-26 Illumina Software, Inc. Generating cluster-specific-signal corrections for determining nucleotide-base calls
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
US20250067765A1 (en) * 2021-12-22 2025-02-27 Nautilus Subsidiary, Inc. Systems and methods for carrying out highly multiplexed bioanalyses
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
WO2023129898A2 (en) 2021-12-27 2023-07-06 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
CA3242624A1 (en) 2021-12-29 2023-07-06 Shannon Whitmore Automatically switching variant analysis model versions for genomic analysis applications
CN118475822A (zh) * 2021-12-30 2024-08-09 伊鲁米那有限公司 成像系统以及相关系统和方法
EP4733410A2 (en) 2022-01-21 2026-04-29 10X Genomics, Inc. Multiple readout signals for analyzing a sample
EP4479928B1 (en) 2022-02-17 2026-04-01 Illumina, Inc. Ai-driven enhancement of motion blurred sequencing images
US20230296516A1 (en) 2022-02-17 2023-09-21 Illumina, Inc. Ai-driven signal enhancement of sequencing images
WO2023164570A1 (en) 2022-02-23 2023-08-31 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
EP4483372B1 (en) 2022-02-25 2026-04-01 Illumina, Inc. Calibration sequences for nucelotide sequencing
CA3224595A1 (en) 2022-02-25 2023-08-31 Steven Norberg Machine-learning models for detecting and adjusting values for nucleotide methylation levels
US12092578B2 (en) 2022-03-29 2024-09-17 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
CN119234045A (zh) 2022-04-06 2024-12-31 10X基因组学有限公司 用于多重细胞分析的方法
CN119053701A (zh) 2022-04-07 2024-11-29 因美纳有限公司 经改变的胞苷脱氨酶和使用方法
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4515547A1 (en) 2022-04-26 2025-03-05 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
US20230348967A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
WO2023215612A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
US20230368866A1 (en) 2022-05-10 2023-11-16 Illumina Software, Inc. Adaptive neural network for nucelotide sequencing
US20230407386A1 (en) 2022-06-09 2023-12-21 Illumina, Inc. Dependence of base calling on flow cell tilt
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
EP4544077A1 (en) 2022-06-21 2025-04-30 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
EP4544558A1 (en) 2022-06-24 2025-04-30 Illumina, Inc. Improving split-read alignment by intelligently identifying and scoring candidate split groups
WO2024006705A1 (en) 2022-06-27 2024-01-04 Illumina Software, Inc. Improved human leukocyte antigen (hla) genotyping
CN119422199A (zh) 2022-06-27 2025-02-11 因美纳有限公司 用于基因型推算模型的加速器
WO2024006769A1 (en) 2022-06-27 2024-01-04 Illumina Software, Inc. Generating and implementing a structural variation graph genome
US20240038327A1 (en) 2022-07-26 2024-02-01 Illumina Software, Inc. Rapid single-cell multiomics processing using an executable file
CN119677856A (zh) 2022-08-12 2025-03-21 10X基因组学有限公司 Puma1聚合酶及其用途
WO2024040060A1 (en) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
EP4328569A1 (en) * 2022-08-23 2024-02-28 NanoTemper Technologies GmbH Measuring high protein concentrations
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
EP4594012A1 (en) 2022-09-26 2025-08-06 Illumina, Inc. Flow cell based motion system calibration and control methods
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
CN118974830A (zh) 2022-09-29 2024-11-15 因美纳有限公司 用于推算靶变体的靶变体参考组
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
EP4594481A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Helicase-cytidine deaminase complexes and methods of use
JP2025534192A (ja) 2022-09-30 2025-10-15 イルミナ インコーポレイテッド 構造バリアントコールを精緻化するための機械学習モデル
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
EP4599449A1 (en) 2022-10-05 2025-08-13 Illumina, Inc. Integrating variant calls from multiple sequencing pipelines utilizing a machine learning architecture
WO2024081649A1 (en) 2022-10-11 2024-04-18 Illumina, Inc. Detecting and correcting methylation values from methylation sequencing assays
EP4602344A1 (en) 2022-10-14 2025-08-20 10X Genomics, Inc. Methods for analysis of biological samples
WO2024102736A1 (en) 2022-11-08 2024-05-16 10X Genomics, Inc. Immobilization methods and compositions for in situ detection
US20240158852A1 (en) 2022-11-16 2024-05-16 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
US12372771B2 (en) 2022-11-18 2025-07-29 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
US12581050B2 (en) 2022-11-23 2026-03-17 10X Genomics, Inc. Optical assemblies for machine vision calibration
WO2024118791A1 (en) 2022-11-30 2024-06-06 Illumina, Inc. Accurately predicting variants from methylation sequencing data
AU2022279500B1 (en) * 2022-12-01 2023-11-23 Provectus IP Pty Ltd Automated cell analyser and method
WO2024124073A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
US20240201182A1 (en) 2022-12-15 2024-06-20 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
WO2024130203A1 (en) 2022-12-16 2024-06-20 10X Genomics, Inc. Methods and compositions for assessing performance
WO2024137860A1 (en) 2022-12-21 2024-06-27 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
EP4646492A1 (en) 2023-01-06 2025-11-12 10X Genomics, Inc. Methods and compositions for in situ analysis of variant sequences
US20240241110A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
IL318086A (en) 2023-01-27 2025-02-01 Illumina Inc Image analysis for flicker correction
WO2024167954A1 (en) 2023-02-06 2024-08-15 Illumina, Inc. Determining and removing inter-cluster light interference
EP4689173A1 (en) 2023-03-24 2026-02-11 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
WO2024206848A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. Tandem repeat genotyping
US20240368678A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
CN119744419A (zh) 2023-05-03 2025-04-01 因美纳有限公司 用于重新校准来自现有测序数据文件的基因型检出的机器学习模型
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
WO2024249940A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Improving structural variant alignment and variant calling by utilizing a structural-variant reference genome
WO2024249973A2 (en) 2023-06-02 2024-12-05 Illumina, Inc. Linking human genes to clinical phenotypes using graph neural networks
CN121359206A (zh) 2023-06-27 2026-01-16 因美纳有限公司 使用甲基化水平估计的变体检出
WO2025006460A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with modified bases
WO2025006874A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Machine-learning model for recalibrating genotype calls corresponding to germline variants and somatic mosaic variants
WO2025006464A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with alternative scatterplots
WO2025006466A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with four labeled nucleotides
WO2025007300A1 (zh) * 2023-07-05 2025-01-09 武汉华大智造科技有限公司 用于基因测序的流路系统及其控制方法和基因测序仪
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
US20250052565A1 (en) 2023-08-11 2025-02-13 Illumina, Inc. Optical alignment targets
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
WO2025054389A1 (en) 2023-09-07 2025-03-13 Illumina, Inc. Identification of methylated cytosine using landmarks
AU2023464289A1 (en) 2023-09-08 2026-03-12 The Regents Of The University Of Michigan MICRORNA-DERIVED RNAs AND POLYPEPTIDES AND USES THEREOF
WO2025072793A1 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
US20250111899A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Predicting insert lengths using primary analysis metrics
WO2025072870A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Tracking and modifying cluster location on nucleotide-sample slides in real time
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025090883A1 (en) 2023-10-27 2025-05-01 Illumina, Inc. Detecting variants in nucleotide sequences based on haplotype diversity
US20250163502A1 (en) 2023-11-22 2025-05-22 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
US20250201342A1 (en) 2023-12-15 2025-06-19 Illumina, Inc. Minimal residual disease (mrd) models for determining likelihoods or probabilities of a subject comprising cancer
US20250201346A1 (en) 2023-12-18 2025-06-19 Illumina, Inc. Using machine learning models for detecting minimum residual disease (mrd) in a subject
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
WO2025137341A1 (en) 2023-12-20 2025-06-26 Illumina, Inc. Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality
WO2025137166A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
US20250210141A1 (en) 2023-12-21 2025-06-26 Illumina, Inc. Enhanced mapping and alignment of nucleotide reads utilizing an improved haplotype data structure with allele-variant differences
WO2025160089A1 (en) 2024-01-26 2025-07-31 Illumina, Inc. Custom multigenome reference construction for improved sequencing analysis of genomic samples
WO2025174774A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Determining offline corrections for sequence specific errors caused by low complexity nucleotide sequences
WO2025174708A1 (en) 2024-02-13 2025-08-21 Illumina, Inc. Design and method for cross-sequencing platform compatibility of flow cells
CN121359209A (zh) 2024-02-28 2026-01-16 因美纳有限公司 用于改进核苷酸读段的映射和比对以及改进基因型检出的个性化单倍型数据库
WO2025193747A1 (en) 2024-03-12 2025-09-18 Illumina, Inc. Machine-learning models for ordering and expediting sequencing tasks or corresponding nucleotide-sample slides
US20250288988A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
WO2025240241A1 (en) 2024-05-13 2025-11-20 Illumina, Inc. Modifying sequencing cycles during a sequencing run to meet customized coverage estimations for a target genomic region
WO2025240918A1 (en) 2024-05-17 2025-11-20 10X Genomics, Inc. Systems and methods for generating codebooks
WO2025240924A1 (en) 2024-05-17 2025-11-20 Illumina, Inc. Blind equalization systems for base calling applications
WO2025250996A2 (en) 2024-05-31 2025-12-04 Illumina, Inc. Call generation and recalibration models for implementing personalized diploid reference haplotypes in genotype calling
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling
WO2025264702A1 (en) 2024-06-18 2025-12-26 10X Genomics, Inc. 3d hydrogels for nucleic acid sequencing
WO2026006774A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2026006771A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Cluster-filtering scores
WO2026030370A1 (en) 2024-07-30 2026-02-05 Illumina, Inc. Generating adaptive intensity value decision boundaries for nucleotide sequencing
WO2026030369A1 (en) 2024-07-31 2026-02-05 10X Genomics, Inc. Methods and compositions for in situ analyte detection
WO2026039468A1 (en) 2024-08-16 2026-02-19 Illumina, Inc. Sequencing illumination adjustment system
WO2026043885A1 (en) 2024-08-20 2026-02-26 10X Genomics, Inc. Methods and systems for preparing a nucleic acid library
WO2026072725A1 (en) 2024-09-25 2026-04-02 Illumina, Inc. Adjusting oligonucleotide-cluster location in images in real time using band-edge spectral regions
WO2026072986A1 (en) 2024-09-27 2026-04-02 Illumina, Inc. Determining offline corrections for sequence specific errors caused by low complexity sequences using a machine learning model
WO2026072464A1 (en) 2024-09-27 2026-04-02 Illumina, Inc. Read mapping enhanced by spatial information
WO2026072436A1 (en) 2024-09-27 2026-04-02 Illumina, Inc. Systems and methods for quantifying sequence read connectivity

Family Cites Families (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4160263A (en) 1978-05-15 1979-07-03 George R. Cogar Dual or multiple objective video microscope for superimposing spaced images
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US6953676B1 (en) * 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
DE19536903C2 (de) * 1995-10-04 1998-09-10 Boehringer Ingelheim Int Vorrichtung zum Haltern eines fluidischen Bauteils
US5803117A (en) 1996-06-10 1998-09-08 Rheodyne, L.P. Multi-route full sweep selection valve
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US20020055100A1 (en) 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
US6399936B1 (en) 1997-12-01 2002-06-04 New Dimension Research Instrument, Inc. Optical confocal device having a common light directing means
US20030036855A1 (en) * 1998-03-16 2003-02-20 Praelux Incorporated, A Corporation Of New Jersey Method and apparatus for screening chemical compounds
US6185030B1 (en) * 1998-03-20 2001-02-06 James W. Overbeck Wide field of view and high speed scanning microscopy
US6012488A (en) 1998-09-17 2000-01-11 Rheodyne, L.P. Segmenting valve
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6326612B1 (en) * 1998-10-13 2001-12-04 Texas Instruments Incorporated System and method for optical sensing utilizing a portable, detachable sensor cartridge
US6424404B1 (en) 1999-01-11 2002-07-23 Kenneth C. Johnson Multi-stage microlens array
US20030215821A1 (en) 1999-04-20 2003-11-20 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
EP1923471B1 (en) 1999-04-20 2012-12-19 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6320174B1 (en) 1999-11-16 2001-11-20 Ikonisys Inc. Composing microscope
US20030091477A1 (en) * 1999-12-22 2003-05-15 Paul Eric A. Flow-thru chip cartridge, chip holder, system & method thereof
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US6526658B1 (en) * 2000-05-23 2003-03-04 Silverbrook Research Pty Ltd Method of manufacture of an ink jet printhead having a moving nozzle with an externally arranged actuator
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
US6603546B1 (en) 2000-07-21 2003-08-05 I.S.S. (Usa) Inc. Rapid high throughput spectrometer and method
US6875973B2 (en) * 2000-08-25 2005-04-05 Amnis Corporation Auto focus for a flow imaging system
US6374684B1 (en) 2000-08-25 2002-04-23 Cepheid Fluid control and processing system
US7514046B2 (en) * 2000-10-31 2009-04-07 Caliper Life Sciences, Inc. Methods and systems for processing microscale devices for reuse
US7378280B2 (en) * 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
JP2002219849A (ja) 2000-11-27 2002-08-06 Riso Kagaku Corp 孔版印刷装置
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US7864369B2 (en) 2001-03-19 2011-01-04 Dmetrix, Inc. Large-area imaging by concatenation with array microscope
AU2002307152A1 (en) 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US7116437B2 (en) 2001-09-14 2006-10-03 Dmetrix Inc. Inter-objective baffle system
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
US6982166B2 (en) 2002-05-16 2006-01-03 Applera Corporation Lens assembly for biological testing
US7193775B2 (en) 2002-05-30 2007-03-20 Dmetrix, Inc. EPI-illumination system for an array microscope
US6987259B2 (en) 2002-05-30 2006-01-17 Dmetrix, Inc. Imaging system with an integrated source and detector array
US7312432B2 (en) 2002-07-08 2007-12-25 Dmetrix, Inc. Single axis illumination for multi-axis imaging system
US7582470B2 (en) * 2002-07-31 2009-09-01 Gen-Probe Incorporated Device for amplifying and detecting a target nucleic acid
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US6950241B1 (en) 2002-09-18 2005-09-27 Dmetrix, Inc. Miniature microscope objective for an array microscope
US7388714B2 (en) 2002-09-18 2008-06-17 Dmetrix, Inc. Independent focus compensation for a multi-axis imaging system
AU2003294224A1 (en) 2002-09-23 2004-05-04 Dmetrix, Inc. Multi-mode scanning imaging system
US7034317B2 (en) 2002-12-17 2006-04-25 Dmetrix, Inc. Method and apparatus for limiting scanning imaging array data to characteristics of interest
US20050221281A1 (en) * 2003-01-08 2005-10-06 Ho Winston Z Self-contained microfluidic biochip and apparatus
WO2005003375A2 (en) 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
US7330574B2 (en) 2003-05-08 2008-02-12 Ometrix, Inc. Best-focus estimation by lateral scanning
US7148043B2 (en) * 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
ATE353313T1 (de) 2004-05-22 2007-02-15 Agilent Technologies Inc Bauteil eines mikrofluidischen ventils
US20080125330A1 (en) * 2004-07-01 2008-05-29 Cornell Research Foundation, Inc. Real-Time Pcr Detection of Microorganisms Using an Integrated Microfluidics Platform
WO2006018044A1 (en) 2004-08-18 2006-02-23 Agilent Technologies, Inc. Microfluidic assembly with coupled microfluidic devices
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
AU2005299590B2 (en) * 2004-10-27 2010-09-16 Cepheid Closed-system multi-stage nucleic acid amplification reactions
FI20055043A0 (fi) * 2005-01-31 2005-01-31 Wallac Oy Sovitelma mikrotiterkaivojen kuvausjärjestelmässä
US7507575B2 (en) * 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
JP5331476B2 (ja) 2005-06-15 2013-10-30 カリダ・ジェノミックス・インコーポレイテッド 遺伝子解析および化学解析用の単分子アレイ
US20090142846A1 (en) 2005-08-11 2009-06-04 Eksigent Technologies, Llc Methods for measuring biochemical reactions
JP5058483B2 (ja) * 2005-09-14 2012-10-24 オリンパス株式会社 生体試料の長期間ないし連続的検出方法
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
JP4742242B2 (ja) * 2005-09-30 2011-08-10 カシオ計算機株式会社 撮像装置及びそのプログラム
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US7329860B2 (en) 2005-11-23 2008-02-12 Illumina, Inc. Confocal imaging methods and apparatus
JP5063616B2 (ja) 2006-02-03 2012-10-31 インテジェニックス インコーポレイテッド マイクロ流体デバイス
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US8900828B2 (en) * 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
US8137626B2 (en) * 2006-05-19 2012-03-20 California Institute Of Technology Fluorescence detector, filter device and related methods
US7641860B2 (en) 2006-06-01 2010-01-05 Nanotek, Llc Modular and reconfigurable multi-stage microreactor cartridge apparatus
US7586674B2 (en) * 2006-10-17 2009-09-08 Hnu-Photonics Compuscope
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
RU2009119425A (ru) * 2006-10-24 2010-11-27 Конинклейке Филипс Электроникс Н.В. (Nl) Система для формирования изображения объекта
US7813013B2 (en) 2006-11-21 2010-10-12 Illumina, Inc. Hexagonal site line scanning method and system
US9322063B2 (en) 2007-01-26 2016-04-26 Illumina, Inc. Efficient biomolecule recycling method and system
US7835871B2 (en) * 2007-01-26 2010-11-16 Illumina, Inc. Nucleic acid sequencing system and method
US8298763B2 (en) 2007-03-02 2012-10-30 Lawrence Livermore National Security, Llc Automated high-throughput flow-through real-time diagnostic system
US8039817B2 (en) * 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
DE102008002509A1 (de) * 2008-06-18 2009-12-31 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Stopped-Flow-Chip
WO2010056945A2 (en) * 2008-11-13 2010-05-20 Shalon Ventures, Inc. Methods and systems for tissue processing and imaging
WO2010062913A2 (en) 2008-11-26 2010-06-03 Illumina, Inc. Methods and systems for analysis of sequencing data
US20100157086A1 (en) * 2008-12-15 2010-06-24 Illumina, Inc Dynamic autofocus method and system for assay imager
US8673627B2 (en) * 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8304704B2 (en) * 2009-07-27 2012-11-06 Sensovation Ag Method and apparatus for autofocus using a light source pattern and means for masking the light source pattern
US20110124111A1 (en) * 2009-08-31 2011-05-26 Life Technologies Corporation Low-volume sequencing system and method of use
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
EP4459241A3 (en) * 2009-12-07 2025-01-01 Meso Scale Technologies, LLC. Assay cartridge reader
EP3358387A1 (en) * 2010-08-27 2018-08-08 The Board of Trustees of The Leland Stanford Junior University Microscopy imaging device with advanced imaging properties
WO2012042226A2 (en) 2010-10-01 2012-04-05 Oxford Nanopore Technologies Limited Biochemical analysis apparatus and rotary valve
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
EP2834622B1 (en) * 2012-04-03 2023-04-12 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Also Published As

Publication number Publication date
IL234864A0 (en) 2014-12-31
IL268205B (en) 2020-03-31
AU2016222420A1 (en) 2016-09-22
CA2867665A1 (en) 2013-10-10
AU2016222420B2 (en) 2019-01-03
US9193996B2 (en) 2015-11-24
WO2013151622A1 (en) 2013-10-10
US10549281B2 (en) 2020-02-04
IL268205A (en) 2019-08-29
US20170246635A1 (en) 2017-08-31
EP2834622A1 (en) 2015-02-11
US20230137970A1 (en) 2023-05-04
AU2021200408A1 (en) 2021-03-18
US20140329694A1 (en) 2014-11-06
BR112014024789B1 (pt) 2021-05-25
ES2949570T3 (es) 2023-09-29
US9650669B2 (en) 2017-05-16
AU2013243998B2 (en) 2016-07-28
US20130260372A1 (en) 2013-10-03
HK1201582A1 (en) 2015-09-04
AU2019200400B2 (en) 2020-10-22
AU2021200408B2 (en) 2023-04-06
IL234864B (en) 2019-08-29
US20200139375A1 (en) 2020-05-07
IN2014DN07992A (https=) 2015-05-01
IL273034B (en) 2021-12-01
MX337140B (es) 2016-02-12
JP2015514218A (ja) 2015-05-18
KR102118211B1 (ko) 2020-06-02
US11565267B2 (en) 2023-01-31
MX2014011165A (es) 2015-03-19
AU2019200400A1 (en) 2019-02-07
KR20150000885A (ko) 2015-01-05
BR122021002358B1 (pt) 2021-10-26
IL273034A (en) 2020-04-30
CN204832037U (zh) 2015-12-02
BR122021002358B8 (pt) 2022-03-03
CA3138752A1 (en) 2013-10-10
EP2834622B1 (en) 2023-04-12
KR102271225B1 (ko) 2021-06-29
AU2013243998A1 (en) 2014-10-09
KR20210080618A (ko) 2021-06-30
CA3138752C (en) 2024-02-06
EP4219012A1 (en) 2023-08-02
KR20200064162A (ko) 2020-06-05
JP6159391B2 (ja) 2017-07-05

Similar Documents

Publication Publication Date Title
CA2867665C (en) Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
WO2009102688A3 (en) Flow cells and manifolds having an electroosmotic pump
GB2538731A8 (en) Methods
MX2015017286A (es) Sistema de fluidos para distribución de reactivo a una celda de de flujo.
CA2886462C (en) Microfluidic lal-reactive substances testing method and apparatus
CN203899622U (zh) 一种微流控芯片
GB2556703A (en) Systems and methods
CA2931582C (en) Microfluidic cartridge for molecular diagnosis, docking station using such a microfluidic cartridge, and process for analyzing a biological sample
WO2013134742A3 (en) Micro-tube particles for microfluidic assays and methods of manufacture
SA518400535B1 (ar) خرطوشة خلية تدفق ذات قوس إحكام طافي
WO2016128843A3 (en) Disposable cartridge for sample fluid analysis
MX2016000506A (es) Metodos y sistemas de transferencia de fluidos con regulacion de presion.
BR112012008026A2 (pt) célula de fluxo óptico de interrogação, e, sistema de purificação.
EA201500369A1 (ru) Ячеистая трубка
WO2010130762A9 (en) Apparatus for and method of automated processing of biological samples
WO2010075568A3 (en) Biological analysis systems, devices and methods
PH12018500959B1 (en) Apparatus and system for biofluid sample dispensing and/or assay
SG170703A1 (en) Microfluidic system for detecting a biological entity in a sample
JP2013545451A5 (https=)
MX384530B (es) Dispositivos, sistemas, metodos y equipos para recibir un hisopo.
WO2012122458A3 (en) A cartridge based system and method for detecting an analyte in a sample
SG10201801084SA (en) Flow Control And Processing Cartridge
WO2014076316A3 (en) Apparatus
WO2015114316A3 (en) Fluidic device comprising immobilised reagents within a chamber
GB201113992D0 (en) Device

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20180129

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 12TH ANNIV.) - STANDARD

Year of fee payment: 12

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20241223

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20241223

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20241223

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 13TH ANNIV.) - STANDARD

Year of fee payment: 13

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20260120

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20260120