CA2829606C - Methods and reagents for creating monoclonal antibodies - Google Patents

Methods and reagents for creating monoclonal antibodies Download PDF

Info

Publication number
CA2829606C
CA2829606C CA2829606A CA2829606A CA2829606C CA 2829606 C CA2829606 C CA 2829606C CA 2829606 A CA2829606 A CA 2829606A CA 2829606 A CA2829606 A CA 2829606A CA 2829606 C CA2829606 C CA 2829606C
Authority
CA
Canada
Prior art keywords
antigen
immunoglobulin
nucleic acid
acid sequences
animal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2829606A
Other languages
English (en)
French (fr)
Other versions
CA2829606A1 (en
Inventor
Roberto Polakiewicz
Wan Cheung Cheung
John Edward RUSH
Sean Andre BEAUSOLEIL
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cell Signaling Technology Inc
Original Assignee
Cell Signaling Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cell Signaling Technology Inc filed Critical Cell Signaling Technology Inc
Publication of CA2829606A1 publication Critical patent/CA2829606A1/en
Application granted granted Critical
Publication of CA2829606C publication Critical patent/CA2829606C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
    • C07K16/082Hepadnaviridae, e.g. hepatitis B virus
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/20Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565Complementarity determining region [CDR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CA2829606A 2011-03-09 2012-03-09 Methods and reagents for creating monoclonal antibodies Active CA2829606C (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201161450922P 2011-03-09 2011-03-09
US61/450,922 2011-03-09
US201161560006P 2011-11-15 2011-11-15
US61/560,006 2011-11-15
US201161566876P 2011-12-05 2011-12-05
US61/566,876 2011-12-05
US201261594729P 2012-02-03 2012-02-03
US61/594,729 2012-02-03
PCT/US2012/028501 WO2012122484A1 (en) 2011-03-09 2012-03-09 Methods and reagents for creating monoclonal antibodies

Publications (2)

Publication Number Publication Date
CA2829606A1 CA2829606A1 (en) 2012-09-13
CA2829606C true CA2829606C (en) 2020-06-02

Family

ID=45922811

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2829606A Active CA2829606C (en) 2011-03-09 2012-03-09 Methods and reagents for creating monoclonal antibodies

Country Status (12)

Country Link
US (2) US9920110B2 (enExample)
EP (2) EP3345922A1 (enExample)
JP (3) JP6211931B2 (enExample)
KR (3) KR20190006083A (enExample)
CN (2) CN103842379A (enExample)
AU (3) AU2012225310B2 (enExample)
CA (1) CA2829606C (enExample)
DK (1) DK2683736T3 (enExample)
ES (1) ES2666301T3 (enExample)
HK (1) HK1256304A1 (enExample)
HU (1) HUE037095T2 (enExample)
WO (1) WO2012122484A1 (enExample)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8685898B2 (en) 2009-01-15 2014-04-01 Imdaptive, Inc. Adaptive immunity profiling and methods for generation of monoclonal antibodies
EP2783214A2 (en) 2011-11-23 2014-10-01 The Board of Regents of The University of Texas System Proteomic identification of antibodies
US10077478B2 (en) 2012-03-05 2018-09-18 Adaptive Biotechnologies Corp. Determining paired immune receptor chains from frequency matched subunits
MX354329B (es) 2012-05-08 2018-02-27 Adaptive Biotechnologies Corp Composiciones y metodos para medir y calibrar el error de amplificacion en reacciones de pcr multiple.
CN104619862B (zh) 2012-06-15 2017-10-31 得克萨斯系统大学董事会 单细胞的多转录物的高通量测序
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
SG10201707394PA (en) * 2013-03-15 2017-10-30 Adaptive Biotechnologies Corp Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
US10208125B2 (en) 2013-07-15 2019-02-19 University of Pittsburgh—of the Commonwealth System of Higher Education Anti-mucin 1 binding agents and uses thereof
JP5924455B2 (ja) * 2013-09-09 2016-05-25 株式会社島津製作所 ペプチド断片の調製方法およびそれに用いられるペプチド断片調製用キット、ならびに分析方法
GB201316644D0 (en) * 2013-09-19 2013-11-06 Kymab Ltd Expression vector production & High-Throughput cell screening
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
WO2015200626A1 (en) * 2014-06-25 2015-12-30 The Rockefeller University Compositions and methods for rapid production of versatile nanobody repertoires
CN105277638A (zh) * 2014-07-22 2016-01-27 沈鹤霄 一种单克隆抗体IgG质谱测序方法
ES2784343T3 (es) 2014-10-29 2020-09-24 Adaptive Biotechnologies Corp Detección simultánea altamente multiplexada de ácidos nucleicos que codifican heterodímeros de receptores inmunes adaptativos emparejados de muchas muestras
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
CN104560980B (zh) * 2015-01-20 2017-04-05 中国人民解放军第三军医大学 基于高通量测序构建鼠BCR轻链Lamda文库的多重PCR引物和方法
CN104560979B (zh) * 2015-01-20 2017-04-05 中国人民解放军第三军医大学 基于高通量测序构建鼠bcr重链文库的多重pcr引物和方法
US11047008B2 (en) 2015-02-24 2021-06-29 Adaptive Biotechnologies Corporation Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
GB2555921B (en) 2015-03-06 2021-09-15 Micromass Ltd Endoscopic tissue identification tool
GB2553918B (en) 2015-03-06 2022-10-12 Micromass Ltd Ambient ionization mass spectrometry imaging platform for direct mapping from bulk tissue
CA2981085A1 (en) 2015-03-06 2016-09-15 Micromass Uk Limited Spectrometric analysis
CN110057901B (zh) * 2015-03-06 2022-07-01 英国质谱公司 拭子和活检样品的快速蒸发电离质谱和解吸电喷雾电离质谱分析
CN108700590B (zh) 2015-03-06 2021-03-02 英国质谱公司 细胞群体分析
US11282688B2 (en) 2015-03-06 2022-03-22 Micromass Uk Limited Spectrometric analysis of microbes
CA2978042A1 (en) 2015-03-06 2016-09-15 Micromass Uk Limited Tissue analysis by mass spectrometry or ion mobility spectrometry
US10513733B2 (en) 2015-03-23 2019-12-24 Board Of Regents, The University Of Texas System High throughout sequencing of paired VH and VL transcripts from B cells secreting antigen-specific antibodies
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
WO2017044885A1 (en) * 2015-09-09 2017-03-16 uBiome, Inc. Method and system for microbiome-derived diagnostics and therapeutics for conditions associated with cerebro-craniofacial health
US11454611B2 (en) 2016-04-14 2022-09-27 Micromass Uk Limited Spectrometric analysis of plants
CN106047857B (zh) * 2016-06-01 2020-04-03 苏州金唯智生物科技有限公司 一种发掘特异性功能抗体的方法
CN107525934A (zh) * 2016-06-21 2017-12-29 张效龙 一种测定血液中抗体氨基酸序列的方法
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
CN110382696B (zh) * 2017-03-07 2024-04-19 豪夫迈·罗氏有限公司 发现替代性抗原特异性抗体变体的方法
AU2018303254B2 (en) * 2017-07-21 2025-05-29 Novimmune Sa Generating multispecific antibody mixtures and methods of uses thereof
CN109425662A (zh) * 2017-08-23 2019-03-05 深圳华大基因研究院 一种鉴定蛋白的方法及系统
WO2019084538A1 (en) 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
CN119881293A (zh) 2018-03-21 2025-04-25 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
JP2020514681A (ja) * 2018-03-29 2020-05-21 深▲セン▼▲達▼▲闥▼科技控股有限公司Cloudminds (Shenzhen) Holdings Co., Ltd. 物質検出方法、装置、電子機器、およびコンピュータ可読記憶媒体
CN108530533B (zh) * 2018-04-13 2020-09-04 吉林大学 猪瘟病毒单克隆抗体hk24及医用用途
EP3894867A4 (en) * 2018-12-11 2022-09-28 Merck Sharp & Dohme Corp. PROCEDURE FOR IDENTIFICATION OF HIGH-AFFINITY MONOCLONAL ANTIBODIES OF HEAVY AND LIGHT CHAIN FROM HIGH-THROUGHPUT SCREENS OF B-CELL AND HYBRIDOME LIBRARIES
JP7516368B2 (ja) * 2019-06-07 2024-07-16 中外製薬株式会社 情報処理システム、情報処理方法、プログラム、及び、抗原結合分子或いはタンパク質を製造する方法
CN110452296A (zh) * 2019-07-16 2019-11-15 福州精锐生物技术有限公司 一种兔单克隆抗体制备方法
MX2022003244A (es) * 2019-09-16 2022-04-26 Regeneron Pharma Metodos de lc-ms para la isotipificacion y cuantificacion de anticuerpos.
CN115175919A (zh) * 2019-12-09 2022-10-11 光行科技株式会社 转换结合剂、其制备方法、和各自使用该转换结合剂的药物组合物、测定试剂盒、以及抗原和抗体测定方法
JP7765090B2 (ja) * 2020-05-01 2025-11-06 ユニバーシティ オブ ピッツバーグ -オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション ナノボディ及びナノボディ親和性を同定するための組成物及び方法
EP4320148A4 (en) * 2021-04-09 2025-05-14 Abterra Biosciences, Inc. METHOD FOR IDENTIFYING ANTIBODIES FROM PROTEIN MIXTURES
IT202100019334A1 (it) * 2021-07-21 2023-01-21 Univ Degli Studi Di Pavia Metodo per l’identificazione dell’intera sequenza della regione variabile delle catene pesanti e leggere di immunoglobuline
CN115894687B (zh) * 2022-03-31 2024-06-07 台州恩泽医疗中心(集团) 一种抗hla-g1,-g2,-g5及hla-g6异构体分子的单克隆抗体及其用途
CN116779037B (zh) * 2022-07-27 2025-08-05 北京理工大学 一种基于从头测序和同源标签图论的抗体序列鉴定方法
CN117849159B (zh) * 2024-01-09 2025-01-07 融智生物科技(青岛)有限公司 M蛋白的检测方法、电子设备及存储介质
CN120214310B (zh) * 2025-05-20 2025-08-19 中国农业大学 一种禽白血病抗原检测试剂盒及其制备方法

Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4485045A (en) 1981-07-06 1984-11-27 Research Corporation Synthetic phosphatidyl cholines useful in forming liposomes
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4544545A (en) 1983-06-20 1985-10-01 Trustees University Of Massachusetts Liposomes containing modified cholesterol for organ targeting
US6548640B1 (en) 1986-03-27 2003-04-15 Btg International Limited Altered antibodies
US5075109A (en) 1986-10-24 1991-12-24 Southern Research Institute Method of potentiating an immune response
US4897268A (en) 1987-08-03 1990-01-30 Southern Research Institute Drug delivery system and method of making the same
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6673776B1 (en) 1989-03-21 2004-01-06 Vical Incorporated Expression of exogenous polynucleotide sequences in a vertebrate, mammal, fish, bird or human
US5013556A (en) 1989-10-20 1991-05-07 Liposome Technology, Inc. Liposomes with enhanced circulation time
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
CA2050918A1 (en) 1990-01-12 1991-07-13 Raju Kucherlapati Generation of xenogeneic antibodies
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
JPH07503132A (ja) 1991-12-17 1995-04-06 ジェンファーム インターナショナル,インコーポレイティド 異種抗体を産生することができるトランスジェニック非ヒト動物
AU688751B2 (en) 1993-01-12 1998-03-19 Biogen Idec Ma Inc. Recombinant anti-VLA4 antibody molecules
US6436908B1 (en) 1995-05-30 2002-08-20 Duke University Use of exogenous β-adrenergic receptor and β-adrenergic receptor kinase gene constructs to enhance myocardial function
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5739277A (en) 1995-04-14 1998-04-14 Genentech Inc. Altered polypeptides with increased half-life
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
WO1999018792A1 (en) 1997-10-10 1999-04-22 Johns Hopkins University Gene delivery compositions and methods
EP1082458A1 (en) 1998-05-01 2001-03-14 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7300753B2 (en) 1998-09-04 2007-11-27 John Rush Immunoaffinity isolation of modified peptides from complex mixtures
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
DE60239962D1 (de) 2001-08-14 2011-06-16 Harvard College Absolute quantifizierung von proteinen und modifizierten formen davon mittels mehrstufiger massenspektrometrie
EP1458736A4 (en) * 2001-12-14 2005-09-14 Lilly Co Eli RECOMBINANT RINDER THROMBIN
GB0130267D0 (en) 2001-12-19 2002-02-06 Neutec Pharma Plc Focussed antibody technology
US7485291B2 (en) 2003-06-03 2009-02-03 Cell Genesys, Inc. Compositions and methods for generating multiple polypeptides from a single vector using a virus derived peptide cleavage site, and uses thereof
CA2526120A1 (en) 2003-06-03 2005-02-24 Cell Genesys, Inc. Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005077106A2 (en) 2004-02-09 2005-08-25 Northeastern University Monoclonal antibody based biomarker discovery and development platform
CA2557841A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20070161001A1 (en) 2004-03-04 2007-07-12 Dena Leshkowitz Quantifying and profiling antibody and t cell receptor gene expression
JO3058B1 (ar) 2005-04-29 2017-03-15 Applied Molecular Evolution Inc الاجسام المضادة لمضادات -اي ال-6,تركيباتها طرقها واستعمالاتها
SI1891088T1 (sl) * 2005-06-10 2012-02-29 Ares Trading Sa Postopek za äśiĺ äśenje il-18 vezavnega proteina
AR059432A1 (es) 2006-02-10 2008-04-09 Genentech Inc Anticuerpos anti-fgf19 y metodos que usan estos
GB0612928D0 (en) 2006-06-29 2006-08-09 Ucb Sa Biological products
CA2657223A1 (en) * 2006-07-07 2008-01-10 Pall Corporation Use of denaturing agents during affinity capture
JP2010515020A (ja) 2006-12-21 2010-05-06 ノバルティス アーゲー 抗体定量法
EP2132229B1 (en) 2007-03-01 2016-05-11 Symphogen A/S Recombinant anti-epidermal growth factor receptor antibody compositions
KR20090127341A (ko) 2007-03-06 2009-12-10 심포젠 에이/에스 호흡기세포 융합 바이러스 감염 치료용 재조합 항체
PL2125894T3 (pl) 2007-03-22 2019-08-30 Biogen Ma Inc. Białka wiążące, w tym przeciwciała, pochodne przeciwciał i fragmenty przeciwciał, które swoiście wiążą się z CD154 i ich zastosowania
EP2336329B8 (en) 2007-06-01 2012-11-21 Omt, Inc. Compositions and methods for inhibiting endogenous immunoglobulin genes and producing transgenis human idiotype antibodies
US7887801B2 (en) 2007-07-13 2011-02-15 Topotarget Germany Ag Optimized DNA and protein sequence of an antibody to improve quality and yield of bacterially expressed antibody fusion proteins
JP4932940B2 (ja) * 2007-11-12 2012-05-16 セラクローン サイエンシーズ, インコーポレイテッド インフルエンザの治療および診断のための組成物および方法
CA2702322A1 (en) 2007-11-22 2009-05-28 Symphogen A/S A method for characterization of a recombinant polyclonal protein
EP2088432A1 (en) 2008-02-11 2009-08-12 MorphoSys AG Methods for identification of an antibody or a target
WO2010002911A2 (en) 2008-06-30 2010-01-07 H. Lee Moffitt Cancer Center And Research Institute, Inc. Methods and materials for monitoring myeloma using quantitative mass spetrometry
LT2346994T (lt) 2008-09-30 2022-03-10 Ablexis, Llc Knock-in pelė, skirta chimerinių antikūnų gamybai
MX2011007660A (es) 2008-12-18 2011-08-17 Kingdon Craig R Animales transgenicos no humanos que expresan anticuerpos humanizados y usos de los mismos.
US8685898B2 (en) 2009-01-15 2014-04-01 Imdaptive, Inc. Adaptive immunity profiling and methods for generation of monoclonal antibodies
TR201818431T4 (tr) 2009-02-25 2019-01-21 Ucb Biopharma Sprl Antikorlar Üretmek İçin Usul
PT2564695E (pt) 2009-07-08 2015-06-03 Kymab Ltd Modelos animais e moléculas terapêuticas
US20110045534A1 (en) 2009-08-20 2011-02-24 Cell Signaling Technology, Inc. Nucleic Acid Cassette For Producing Recombinant Antibodies
US8293483B2 (en) 2009-09-11 2012-10-23 Epitomics, Inc. Method for identifying lineage-related antibodies
ES2984483T3 (es) 2010-03-31 2024-10-29 Ablexis Llc Ingeniería genética de ratones para la producción de anticuerpos quiméricos
US9234240B2 (en) 2010-05-07 2016-01-12 The Board Of Trustees Of The Leland Stanford Junior University Measurement and comparison of immune diversity by high-throughput sequencing
CA2799746C (en) 2010-05-17 2020-11-24 Sai Reddy Rapid isolation of monoclonal antibodies from animals
CN103228130B (zh) 2010-06-17 2016-03-16 科马布有限公司 动物模型及治疗分子

Also Published As

Publication number Publication date
KR20140010436A (ko) 2014-01-24
EP3345922A1 (en) 2018-07-11
EP2683736B1 (en) 2018-01-17
AU2012225310B2 (en) 2017-08-03
DK2683736T3 (en) 2018-04-23
AU2019216660A1 (en) 2019-09-05
AU2012225310A1 (en) 2013-09-26
EP2683736A1 (en) 2014-01-15
JP2014509853A (ja) 2014-04-24
CN103842379A (zh) 2014-06-04
AU2017245395B2 (en) 2019-05-16
AU2017245395A1 (en) 2017-11-02
KR20190006083A (ko) 2019-01-16
AU2017245395A2 (en) 2017-11-23
US20120308555A1 (en) 2012-12-06
JP6557709B2 (ja) 2019-08-07
HUE037095T2 (hu) 2018-08-28
WO2012122484A1 (en) 2012-09-13
KR101938343B1 (ko) 2019-01-14
CN108220297A (zh) 2018-06-29
ES2666301T3 (es) 2018-05-03
KR20190107761A (ko) 2019-09-20
JP6211931B2 (ja) 2017-10-11
JP2019195335A (ja) 2019-11-14
CA2829606A1 (en) 2012-09-13
US20190002532A1 (en) 2019-01-03
JP2018027090A (ja) 2018-02-22
HK1256304A1 (en) 2019-09-20
US9920110B2 (en) 2018-03-20

Similar Documents

Publication Publication Date Title
CA2829606C (en) Methods and reagents for creating monoclonal antibodies
Nguyen et al. Functional heavy-chain antibodies in Camelidae
EP2310413B1 (en) Camelid derived antigen binding polypeptides
CN114126646B (zh) 基于序列的高通量方法产生骆驼抗体以高分辨率覆盖广泛表位
EP3421592A1 (en) Identification of polynucleotides associated with a sample
JP2018148915A (ja) 抗vasa抗体、ならびにその産生法および使用法
Chassagne et al. A high-affinity macaque antibody Fab with human-like framework regions obtained from a small phage display immune library
HK1193825A (en) Methods and reagents for creating monoclonal antibodies
HK1193825B (en) Methods and reagents for creating monoclonal antibodies
TW202229328A (zh) 抗原特異性抗體之鑑定及產生
US20130109586A1 (en) Generation of antibodies to an epitope of interest that contains a phosphomimetic amino acid
WO2021258114A1 (en) Process for direct readout of immunoglobulins
US12331133B2 (en) Therapeutic antibodies for treating lung cancer
CN111201239A (zh) 用于开发特异于表位翻译后修饰状态的抗体的方法和组合物
CN117015302A (zh) 抗原特异性抗体的鉴定和产生
WO2025238133A1 (en) Multispecific antibody with binding specificity for il-11 and il-17
Almagro et al. Novel approaches in discovery and design of antibody-based therapeutics
IL210002A (en) Polypeptides that bind to antigen

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20161212