CA2769361A1 - Cell culture medium for adamts protein expression - Google Patents

Cell culture medium for adamts protein expression Download PDF

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Publication number
CA2769361A1
CA2769361A1 CA2769361A CA2769361A CA2769361A1 CA 2769361 A1 CA2769361 A1 CA 2769361A1 CA 2769361 A CA2769361 A CA 2769361A CA 2769361 A CA2769361 A CA 2769361A CA 2769361 A1 CA2769361 A1 CA 2769361A1
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CA
Canada
Prior art keywords
adamts
protein
culture
culture medium
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2769361A
Other languages
English (en)
French (fr)
Inventor
Leopold Grillberger
Alexandra Spenger
Meinhard Hasslacher
Rana Grillberger
Manfred Reiter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter Healthcare SA
Baxter International Inc
Original Assignee
Baxter Healthcare SA
Baxter International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Healthcare SA, Baxter International Inc filed Critical Baxter Healthcare SA
Publication of CA2769361A1 publication Critical patent/CA2769361A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0681Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0684Cells of the urinary tract or kidneys
    • C12N5/0686Kidney cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489Metalloendopeptidases (3.4.24)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24Metalloendopeptidases (3.4.24)
    • C12Y304/24087ADAMTS13 endopeptidase (3.4.24.87)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/12Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14Calcium; Ca chelators; Calcitonin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/22Zinc; Zn chelators
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95Protein-free medium and culture conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/99Serum-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
CA2769361A 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression Abandoned CA2769361A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31
US61/230,477 2009-07-31
PCT/US2010/044020 WO2011014838A1 (en) 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression

Publications (1)

Publication Number Publication Date
CA2769361A1 true CA2769361A1 (en) 2011-02-03

Family

ID=42668009

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2769361A Abandoned CA2769361A1 (en) 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression

Country Status (21)

Country Link
US (7) US8313926B2 (OSRAM)
EP (1) EP2459702B1 (OSRAM)
JP (5) JP5451884B2 (OSRAM)
KR (5) KR101808496B1 (OSRAM)
CN (2) CN104962539B (OSRAM)
AU (1) AU2010278751B2 (OSRAM)
BR (2) BR122021005965B1 (OSRAM)
CA (1) CA2769361A1 (OSRAM)
CO (1) CO6501191A2 (OSRAM)
EA (1) EA023193B1 (OSRAM)
ES (1) ES2583258T3 (OSRAM)
HR (1) HRP20160783T1 (OSRAM)
HU (1) HUE029110T2 (OSRAM)
IN (1) IN2012DN00801A (OSRAM)
MX (2) MX2012001261A (OSRAM)
NZ (1) NZ597727A (OSRAM)
PL (1) PL2459702T3 (OSRAM)
PT (1) PT2459702T (OSRAM)
SG (1) SG178194A1 (OSRAM)
SI (1) SI2459702T1 (OSRAM)
WO (1) WO2011014838A1 (OSRAM)

Cited By (1)

* Cited by examiner, † Cited by third party
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EP3909977A1 (en) * 2010-07-08 2021-11-17 Takeda Pharmaceutical Company Limited Method of producing recombinant adamts13 in cell culture

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KR102418824B1 (ko) * 2013-10-14 2022-07-11 아레스 트레이딩 에스.아. 포유동물의 고성능 유가식 배양을 위한 신규 배지
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3909977A1 (en) * 2010-07-08 2021-11-17 Takeda Pharmaceutical Company Limited Method of producing recombinant adamts13 in cell culture

Also Published As

Publication number Publication date
KR101808496B1 (ko) 2017-12-12
US20190071660A1 (en) 2019-03-07
US8313926B2 (en) 2012-11-20
SG178194A1 (en) 2012-03-29
US8759026B2 (en) 2014-06-24
JP2013500724A (ja) 2013-01-10
CN104962539A (zh) 2015-10-07
JP6567707B2 (ja) 2019-08-28
JP5936232B2 (ja) 2016-06-22
KR101912552B1 (ko) 2018-10-26
US20110086413A1 (en) 2011-04-14
JP7083059B2 (ja) 2022-06-09
CN102549145A (zh) 2012-07-04
BR112012002151B1 (pt) 2021-07-06
KR20150138425A (ko) 2015-12-09
JP2014061005A (ja) 2014-04-10
ES2583258T3 (es) 2016-09-20
JP6511007B2 (ja) 2019-05-08
JP5451884B2 (ja) 2014-03-26
CN104962539B (zh) 2019-09-17
US20170051270A1 (en) 2017-02-23
US10072254B2 (en) 2018-09-11
CO6501191A2 (es) 2012-08-15
NZ597727A (en) 2013-08-30
JP2019201661A (ja) 2019-11-28
KR101573972B1 (ko) 2015-12-02
HRP20160783T1 (hr) 2016-08-12
EP2459702A1 (en) 2012-06-06
JP2018093881A (ja) 2018-06-21
US20140329294A1 (en) 2014-11-06
KR101574056B1 (ko) 2015-12-02
US9441216B2 (en) 2016-09-13
US20130071906A1 (en) 2013-03-21
US20200308566A1 (en) 2020-10-01
CN102549145B (zh) 2015-05-20
EA201270224A1 (ru) 2012-09-28
JP2016144471A (ja) 2016-08-12
AU2010278751B2 (en) 2014-05-15
HUE029110T2 (en) 2017-02-28
WO2011014838A1 (en) 2011-02-03
MX344970B (es) 2017-01-12
JP6883071B2 (ja) 2021-06-09
MX2012001261A (es) 2012-05-22
US20150329848A1 (en) 2015-11-19
PL2459702T3 (pl) 2016-12-30
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
JP2021112210A (ja) 2021-08-05
PT2459702T (pt) 2016-08-02
BR122021005965B1 (pt) 2022-01-25
KR20170140411A (ko) 2017-12-20
EP2459702B1 (en) 2016-05-04
HK1171790A1 (zh) 2013-04-05
IN2012DN00801A (OSRAM) 2015-06-26
EA023193B1 (ru) 2016-05-31
KR20170081747A (ko) 2017-07-12
KR101756354B1 (ko) 2017-07-26
AU2010278751A1 (en) 2012-02-09
KR20150044981A (ko) 2015-04-27
US11254921B2 (en) 2022-02-22
BR112012002151A2 (pt) 2020-03-03
US9127265B2 (en) 2015-09-08
KR20120046301A (ko) 2012-05-09

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