CA2742593C - Chimeric dna polymerases - Google Patents
Chimeric dna polymerases Download PDFInfo
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- CA2742593C CA2742593C CA2742593A CA2742593A CA2742593C CA 2742593 C CA2742593 C CA 2742593C CA 2742593 A CA2742593 A CA 2742593A CA 2742593 A CA2742593 A CA 2742593A CA 2742593 C CA2742593 C CA 2742593C
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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| PCT/US2009/063166 WO2010062776A2 (en) | 2008-11-03 | 2009-11-03 | Chimeric dna polymerases |
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| US9315787B2 (en) | 2011-01-14 | 2016-04-19 | Kapa Biosystems, Inc. | Modified DNA polymerases for improved amplification |
| CN102392000A (zh) * | 2011-11-07 | 2012-03-28 | 李志茹 | 一种耐高温Pyrolobus聚合酶及其高效表达质粒和应用 |
| US9309501B2 (en) | 2012-12-24 | 2016-04-12 | Industrial Technology Research Institute | Isolated DNA polymerases, kits and applications thereof |
| CN104250641B (zh) * | 2013-06-28 | 2017-09-01 | 北京福安华生物科技有限公司 | 一种高保真dna聚合酶及其制备和应用 |
| JP6428997B2 (ja) * | 2013-08-06 | 2018-11-28 | 東洋紡株式会社 | 核酸増幅法 |
| EP3161154B1 (en) * | 2014-06-27 | 2020-04-15 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| US9963687B2 (en) | 2014-08-27 | 2018-05-08 | New England Biolabs, Inc. | Fusion polymerase and method for using the same |
| JP6594955B2 (ja) * | 2014-08-27 | 2019-10-23 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | シントン形成 |
| CN108064278B (zh) * | 2014-11-14 | 2022-02-08 | 亿明达股份有限公司 | 聚合酶 |
| JP6619152B2 (ja) * | 2015-05-08 | 2019-12-11 | 国立研究開発法人産業技術総合研究所 | フラビンアデニンジヌクレオチド依存型グルコース脱水素酵素活性を有するキメラタンパク質 |
| CN106867978A (zh) * | 2015-12-10 | 2017-06-20 | 江苏众红生物工程创药研究院有限公司 | 一种重组高保真dna聚合酶及其编码基因和表达方法 |
| JP6969089B2 (ja) * | 2015-12-11 | 2021-11-24 | 東洋紡株式会社 | 核酸増幅用組成物及び核酸増幅法 |
| JP6980375B2 (ja) * | 2015-12-11 | 2021-12-15 | 東洋紡株式会社 | 核酸増幅用試薬及び核酸増幅法 |
| LT3402880T (lt) | 2016-01-15 | 2024-03-12 | Thermo Fisher Scientific Baltics Uab | Termofiliniai dnr polimerazės mutantai |
| EP4067491A1 (en) | 2016-01-19 | 2022-10-05 | Board of Regents, The University of Texas System | Thermostable reverse transcriptase |
| EP3458597B1 (en) | 2016-05-18 | 2022-09-07 | Roche Diagnostics GmbH | Quantitative real time pcr amplification using an electrowetting-based device |
| FR3052462A1 (fr) * | 2016-06-14 | 2017-12-15 | Dna Script | Variants d'une adn polymerase de la famille polx |
| CN106011100A (zh) * | 2016-08-08 | 2016-10-12 | 吴江近岸蛋白质科技有限公司 | 一种Pfu DNA聚合酶及其制备方法 |
| EP3555272A2 (en) | 2016-12-19 | 2019-10-23 | Quantum-si Incorporated | Polymerizing enzymes for sequencing reactions |
| CN106754816B (zh) * | 2017-02-24 | 2020-10-27 | 依科赛生物科技（太仓）有限公司 | 一种高保真快速扩增融合酶及其制备方法 |
| US11618891B2 (en) | 2017-06-26 | 2023-04-04 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| AU2018292104B2 (en) | 2017-06-30 | 2025-03-27 | Codexis, Inc. | T7 RNA polymerase variants |
| JP7588391B2 (ja) | 2017-06-30 | 2024-11-22 | コデクシス， インコーポレイテッド | Ｔ７ ｒｎａポリメラーゼバリアント |
| EP4530346A3 (en) * | 2017-07-12 | 2025-06-11 | Genecast Co., Ltd. | Dna polymerase with increased gene mutation specificity and pcr buffer composition for increasing activity thereof |
| WO2019084538A1 (en) | 2017-10-27 | 2019-05-02 | Board Of Regents, The University Of Texas System | TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF |
| WO2019143622A1 (en) | 2018-01-19 | 2019-07-25 | Bio-Rad Laboratories, Inc. | Mutant dna polymerases |
| EP3862427A4 (en) * | 2018-09-03 | 2022-04-27 | BGI Shenzhen | RECOMBINANT KOD POLYMERASE |
| KR20210084349A (ko) * | 2018-10-31 | 2021-07-07 | 일루미나, 인코포레이티드 | 중합효소, 조성물, 및 사용 방법 |
| SG11202012559SA (en) | 2018-12-05 | 2021-01-28 | Illumina Inc | Polymerases, compositions, and methods of use |
| CN109628424B (zh) * | 2018-12-31 | 2022-04-26 | 苏州近岸蛋白质科技股份有限公司 | 一种新型嵌合dna聚合酶及其制备方法 |
| US20220090194A1 (en) * | 2019-01-09 | 2022-03-24 | 3Cr Bioscience Ltd. | Method for detecting a nucleic acid sequence |
| GB2587177A (en) * | 2019-01-09 | 2021-03-24 | 3Cr Bioscience Ltd | Polymerase |
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| CN114829593B (zh) * | 2019-12-23 | 2023-11-14 | 深圳华大生命科学研究院 | 嵌合dna聚合酶及其应用 |
| EP4103747A4 (en) * | 2020-02-15 | 2024-03-13 | Agency for Science, Technology and Research | A method to amplify a nucleic acid |
| CN113604450B (zh) * | 2021-08-18 | 2023-08-18 | 华南理工大学 | 一种kod dna聚合酶突变体及其制备方法与应用 |
| CN113755465A (zh) * | 2021-09-23 | 2021-12-07 | 武汉爱博泰克生物科技有限公司 | 嵌合体dna聚合酶及其制备方法 |
| EP4435098A4 (en) * | 2021-11-15 | 2025-10-01 | Bgi Shenzhen | CHIMERIC DNA POLYMERASE AND ITS USE |
| CN119736271A (zh) * | 2024-12-27 | 2025-04-01 | 安序源生物科技(深圳)有限公司 | 一种phi29 DNA聚合酶突变体及其用途 |
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| JP2017029172A (ja) | 2017-02-09 |
| EP2927317B1 (en) | 2018-12-19 |
| EP2352818A4 (en) | 2011-10-12 |
| HK1216032A1 (en) | 2016-10-07 |
| WO2010062776A2 (en) | 2010-06-03 |
| US9023633B2 (en) | 2015-05-05 |
| ES2716402T3 (es) | 2019-06-12 |
| EP2352818B1 (en) | 2016-02-10 |
| JP6150847B2 (ja) | 2017-06-21 |
| EP2927317A1 (en) | 2015-10-07 |
| US20140363848A1 (en) | 2014-12-11 |
| CN102257136A (zh) | 2011-11-23 |
| US20120115188A1 (en) | 2012-05-10 |
| US9388396B2 (en) | 2016-07-12 |
| ES2571446T3 (es) | 2016-05-25 |
| EP2352818A2 (en) | 2011-08-10 |
| JP2012507986A (ja) | 2012-04-05 |
| WO2010062776A3 (en) | 2010-08-19 |
| CA2742593A1 (en) | 2010-06-03 |
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