CA2647476C - Phytase variants - Google Patents

Phytase variants Download PDF

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Publication number
CA2647476C
CA2647476C CA2647476A CA2647476A CA2647476C CA 2647476 C CA2647476 C CA 2647476C CA 2647476 A CA2647476 A CA 2647476A CA 2647476 A CA2647476 A CA 2647476A CA 2647476 C CA2647476 C CA 2647476C
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CA
Canada
Prior art keywords
phytase
seq
variant
composition
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2647476A
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English (en)
French (fr)
Other versions
CA2647476A1 (en
Inventor
Leonardo De Maria
Carsten Andersen
Lars Kobberoee Skov
Mikael Blom Soerensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
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Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of CA2647476A1 publication Critical patent/CA2647476A1/en
Application granted granted Critical
Publication of CA2647476C publication Critical patent/CA2647476C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
CA2647476A 2006-04-04 2007-03-19 Phytase variants Active CA2647476C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200600484 2006-04-04
DKPA200600484 2006-04-04
DKPA200600581 2006-04-25
DKPA200600581 2006-04-25
PCT/DK2007/000135 WO2007112739A1 (en) 2006-04-04 2007-03-19 Phytase variants

Publications (2)

Publication Number Publication Date
CA2647476A1 CA2647476A1 (en) 2007-10-11
CA2647476C true CA2647476C (en) 2016-08-16

Family

ID=38123737

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2647476A Active CA2647476C (en) 2006-04-04 2007-03-19 Phytase variants

Country Status (11)

Country Link
US (4) US8460656B2 (cg-RX-API-DMAC7.html)
EP (2) EP2365064B1 (cg-RX-API-DMAC7.html)
JP (1) JP5221516B2 (cg-RX-API-DMAC7.html)
AU (1) AU2007234177B2 (cg-RX-API-DMAC7.html)
BR (1) BRPI0709732B1 (cg-RX-API-DMAC7.html)
CA (1) CA2647476C (cg-RX-API-DMAC7.html)
DK (1) DK2365064T3 (cg-RX-API-DMAC7.html)
ES (2) ES2531434T3 (cg-RX-API-DMAC7.html)
MX (1) MX2008012632A (cg-RX-API-DMAC7.html)
PL (1) PL2365064T3 (cg-RX-API-DMAC7.html)
WO (1) WO2007112739A1 (cg-RX-API-DMAC7.html)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
AU2007234177B2 (en) 2006-04-04 2012-11-08 Novozymes A/S Phytase variants
US8540984B2 (en) 2006-08-03 2013-09-24 Cornell Research Foundation, Inc. Phytases with improved thermal stability
DK2617814T3 (da) 2006-09-21 2016-02-22 Basf Enzymes Llc Phytaser, nukleinsyrer, der koder for disse, og fremgangsmåder til frembringelse og anvendelse heraf
MX2011003132A (es) 2008-09-26 2011-04-21 Novozymes As Variantes de fitasa de hafnia.
BRPI1008803A2 (pt) 2009-03-20 2015-08-25 Novozymes As Método para fabricar uma bebida não alcoólica
AU2010249500B2 (en) * 2009-05-21 2016-03-24 Basf Enzymes Llc Phytases, nucleic acids encoding them and methods for making and using them
AU2013204082B2 (en) * 2009-05-21 2016-03-31 Basf Enzymes Llc Phytases, nucleic acids encoding them and methods for making and using them
DK2491120T3 (en) 2009-10-22 2016-03-21 Basf Se Synthetic phytasevarianter
RU2472855C2 (ru) * 2009-12-15 2013-01-20 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) МУТАНТНАЯ РЕКОМБИНАНТНАЯ ТЕРМОСТАБИЛЬНАЯ ФИТАЗА (ВАРИАНТЫ), ФРАГМЕНТ ДНК, КОДИРУЮЩИЙ УКАЗАННУЮ ФИТАЗУ (ВАРИАНТЫ), ШТАММ Pichia pastoris - ПРОДУЦЕНТ УКАЗАННОЙ ФИТАЗЫ (ВАРИАНТЫ)
WO2011117406A1 (en) * 2010-03-26 2011-09-29 Novozymes A/S Thermostable phytase variants
CA2794095C (en) * 2010-03-26 2021-02-16 Novozymes A/S Thermostable phytase variants
CN102917600B (zh) * 2010-03-26 2015-07-08 诺维信公司 热稳定性肌醇六磷酸酶变体
BR112013021017B1 (pt) 2011-02-18 2020-04-07 Dupont Nutrition Biosci Aps composição de aditivo de ração, seu método de preparo, método para preparar uma ração e pré mistura
GB201102857D0 (en) 2011-02-18 2011-04-06 Danisco Feed additive composition
GB201102865D0 (en) 2011-02-18 2011-04-06 Danisco Feed additive composition
BR112013027130B1 (pt) 2011-04-21 2021-02-23 Basf Se fitase, aditivo de alimentação animal, alimentação animal e usos de uma fitase
BR112013026496A2 (pt) 2011-04-21 2016-11-29 Basf Se fitase, sequência de ácidos nucleicos isolados, vetor de expressão recombinante, célula hospedeira recombinante, organismo de produção recombinante, aditivo de ração animal, ração animal, e, uso de uma fitase
DK2729809T3 (en) 2011-07-07 2019-02-04 Dupont Nutrition Biosci Aps ASSAY
GB201213801D0 (en) 2012-08-03 2012-09-12 Dupont Nutrition Biosci Aps Feed additive composition
CA2904367A1 (en) 2013-03-08 2014-09-12 Keck Graduate Institute Of Applied Life Sciences Yeast promoters from pichia pastoris
EP3839055B1 (en) * 2013-03-08 2023-08-23 Biogrammatics, Inc. Yeast promoters for protein expression
CA2923934C (en) 2013-09-11 2023-09-26 Novozymes A/S Processes for producing fermentation products
BR112016029894B1 (pt) 2014-06-27 2022-08-30 Novozymes A/S Método para aumentar o ganho de peso e/ou melhorar a razão de corversão de alimentos de animais de fazenda
EP3970506A1 (en) * 2015-07-02 2022-03-23 Novozymes A/S Animal feed compositions comprising gh25 lysozyme and ec 3.1.3.26 phytase and uses thereof
WO2017112540A1 (en) 2015-12-22 2017-06-29 Novozymes A/S Processes for producing fermentation products
TWI615471B (zh) * 2016-02-18 2018-02-21 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI591180B (zh) 2016-02-18 2017-07-11 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI607088B (zh) * 2016-02-18 2017-12-01 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI616532B (zh) * 2016-02-18 2018-03-01 基酵生物科技股份有限公司 提升耐熱性的植酸酶
BR112020004476A2 (pt) 2017-09-15 2020-09-08 Novozymes A/S processo para produção de um produto de fermentação, e, uso de uma combinação de enzimas
US11326187B2 (en) 2017-10-23 2022-05-10 Novozymes A/S Processes for producing a fermentation product
BR112020016068A2 (pt) 2018-02-08 2020-12-08 Danisco Us Inc. Partículas cera termicamente resistente matriz para encapsulamento de enzima
WO2019209623A1 (en) 2018-04-26 2019-10-31 Danisco Us Inc Method for increasing stability of phytase in a solid composition and a granule composition comprising phosphate and phytase
MX2020012754A (es) 2018-05-31 2021-02-26 Novozymes As Procesos para mejorar el crecimiento y la productividad de la levadura.
RU2706086C1 (ru) * 2018-10-29 2019-11-13 Акционерное Общество "Биоамид" Мутантная рекомбинантная термостабильная фитаза
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
WO2020106796A1 (en) 2018-11-20 2020-05-28 Dupont Nutrition Biosciences Aps ENGINEERED ROBUST HIGH Tm-PHYTASE CLADE POLYPEPTIDES AND FRAGMENTS THEREOF
BR112021014873A2 (pt) 2019-01-31 2021-10-05 Novozymes A/S Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação
EP3996519A1 (en) 2019-07-09 2022-05-18 DuPont Nutrition Biosciences ApS Fat coated particulate enzyme compositions
CA3144423A1 (en) 2019-08-05 2021-02-11 Kurt Creamer Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct
US20220330576A1 (en) 2019-08-16 2022-10-20 Dupont Nutrition Biosciences Aps Compositions for gut health
WO2021046073A1 (en) 2019-09-05 2021-03-11 Dupont Nutrition Biosciences Aps Feed composition
EP4048081A2 (en) 2019-10-21 2022-08-31 DuPont Nutrition Biosciences ApS Compositions for gut health
AR120775A1 (es) 2019-12-16 2022-03-16 Novozymes As Procesos para obtener productos de fermentación
BR112022015553A2 (pt) 2020-02-07 2022-10-04 Dupont Nutrition Biosci Aps Composições de ração para saúde animal
BR112022017034A2 (pt) 2020-02-28 2022-12-13 Dupont Nutrition Biosci Aps Composições de ração
EP4196575A1 (en) 2020-08-13 2023-06-21 Novozymes A/S Phytase variants and polynucleotides encoding same
CN117241678A (zh) 2020-10-16 2023-12-15 杜邦营养生物科学有限公司 用于动物健康的饲料组合物
WO2022169933A2 (en) 2021-02-03 2022-08-11 Dupont Nutrition Biosciences Aps Compositions for gut health
WO2023049488A1 (en) 2021-09-27 2023-03-30 Dupont Nutrition Biosciences Aps Feed additive compositions and methods for using the same
CN120380139A (zh) 2022-12-19 2025-07-25 诺维信公司 具有乙酰木聚糖酯酶活性的碳水化合物酯酶家族3(ce3)多肽和编码其的多核苷酸
EP4638768A2 (en) 2022-12-19 2025-10-29 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
CN120693401A (zh) 2022-12-19 2025-09-23 诺维信公司 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途
CN120380158A (zh) 2022-12-19 2025-07-25 诺维信公司 用于降低发酵产物生产方法后端的浆体粘度的方法
WO2024137246A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
CN121001575A (zh) 2023-04-28 2025-11-21 国际营养与健康丹麦有限公司 反刍动物饲料添加剂组合物
AU2024303961A1 (en) 2023-06-13 2025-12-11 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025059013A1 (en) 2023-09-11 2025-03-20 International N&H Denmark Aps Bacillus-based components for inhibiting or delaying the growth of enterococcus spp. in animals
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization
WO2025217017A1 (en) 2024-04-08 2025-10-16 Novozymes A/S Compositions and methods for increasing phosphorous availability

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
IL97645A (en) 1990-03-23 1997-03-18 Gist Brocades Nv Production of enzymes in seeds and their use
WO1992010755A1 (en) 1990-12-05 1992-06-25 Novo Nordisk A/S Proteins with changed epitopes and methods for the production thereof
DK91192D0 (da) 1992-07-10 1992-07-10 Novo Nordisk As Protein
JPH0795946B2 (ja) 1993-08-19 1995-10-18 イチビキ株式会社 フィターゼ及びその製造法
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5689054A (en) 1994-03-17 1997-11-18 The United States Of America As Represented By The Secretary Of Agriculture Low phytic acid mutants and selection thereof
DE4422198C2 (de) 1994-06-24 1997-08-28 Audi Ag Verfahren zum Steuern der elektrischen Beheizung eines Katalysators
EP1559776A3 (en) 1994-06-30 2006-01-11 Novozymes Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system and promoters and terminators for use therein
US5830732A (en) 1994-07-05 1998-11-03 Mitsui Toatsu Chemicals, Inc. Phytase
JP3570784B2 (ja) * 1994-07-05 2004-09-29 三井化学株式会社 新規なるフィターゼ
EP0793726A1 (en) 1994-11-24 1997-09-10 Novo Nordisk A/S A process for producing polypeptides with reduced allergenicity
FI972443L (fi) 1994-12-07 1997-06-09 Novo Nordisk As Polypeptidi, jonka allergeenisuus on vähentynyt
EP0954572A1 (en) 1997-01-10 1999-11-10 Novo Nordisk A/S Enzyme coupled with polymeric molecules for skin care
AU740207B2 (en) 1997-02-06 2001-11-01 Novozymes A/S Polypeptide-polymer conjugates having added and/or removed attachment groups
DK1002064T3 (da) 1997-06-25 2008-02-04 Novozymes As Modificeret polypeptid
NZ330940A (en) * 1997-07-24 2000-02-28 F Production of consensus phytases from fungal origin using computer programmes
US6720014B1 (en) 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
EP1066373B1 (en) 1998-03-23 2009-01-14 Novozymes A/S Phytase variants
DE69907240T2 (de) 1998-10-02 2003-11-27 Novozymes A/S, Bagsvaerd Feste phytase-zusammensetzungen
JP2002527059A (ja) 1998-10-13 2002-08-27 ノボザイムス アクティーゼルスカブ 低められた免疫応答を有する修飾されたポリペプチド
ATE365210T1 (de) 1998-10-30 2007-07-15 Novozymes As Glykosylierte proteine mit reduzierter allergenität
CA2348938A1 (en) 1998-10-30 2000-05-11 Novozymes A/S Low allergenic protein variants
WO2000064247A1 (en) 1999-04-23 2000-11-02 University Of Guelph Transgenic animals expressing salivary proteins
KR100790918B1 (ko) * 1999-11-18 2008-01-03 코넬 리서치 파운데이션 인코포레이티드 에스케리챠 콜리 피타아제의 부위-지향성 돌연변이유발
WO2001058275A2 (en) 2000-02-08 2001-08-16 F Hoffmann-La Roche Ag Use of acid-stable subtilisin proteases in animal feed
EP2258852A1 (en) 2000-04-28 2010-12-08 Novozymes A/S Lipolytic enzyme variant
WO2002090384A2 (en) 2001-05-04 2002-11-14 Novozymes A/S Antimicrobial polypeptide from aspergillus niger
DE60215534T2 (de) 2001-11-20 2007-09-20 Novozymes A/S Antimikrobielle polypeptide aus pseudoplectania nigrella
JP2005513042A (ja) 2001-12-03 2005-05-12 ノボザイムス アクティーゼルスカブ スタチン様化合物
MXPA04011224A (es) 2002-05-30 2005-01-25 Basf Ag Fitasas modificadas.
CN101792749B (zh) * 2002-08-12 2012-09-05 金克克国际有限公司 大肠杆菌AppA肌醇六磷酸酶突变体
US7442398B2 (en) * 2003-03-25 2008-10-28 Republic Of National Fisheries Research And Development Institute Phytase produced from Citrobacter braakii
JP2005137293A (ja) 2003-11-07 2005-06-02 Mitsui Chemicals Inc コドン改変と細胞外分泌の組合せにより外来フィターゼを植物で生産する方法
EP1809747B1 (en) * 2004-10-04 2016-12-14 Novozymes A/S Polypeptides having phytase activity and polynucleotides encoding same
AU2005290934C1 (en) 2004-10-04 2013-05-09 Dupont Nutrition Biosciences Aps Citrobacter freundii phytase and homologues
AR050895A1 (es) 2004-10-04 2006-11-29 Novozymes As Polipeptidos que tienen actividad de fitasa y polinucleotidos que los codifican
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
JP2006136314A (ja) 2004-10-12 2006-06-01 Mitsui Chemicals Inc 新規改変フィターゼ
KR100663112B1 (ko) * 2004-10-29 2007-01-02 주식회사 기성기전 절전형 전자개폐기
WO2006063588A1 (en) * 2004-12-13 2006-06-22 Novozymes A/S Polypeptides having acid phosphatase activity and polynucleotides encoding same
FR2888250B1 (fr) * 2005-07-08 2007-08-17 Adisseo France Sas Soc Par Act Phytase de debaryomyces castellii
AU2007234177B2 (en) 2006-04-04 2012-11-08 Novozymes A/S Phytase variants

Also Published As

Publication number Publication date
US8877471B2 (en) 2014-11-04
EP2365064B1 (en) 2014-12-24
US20160362666A1 (en) 2016-12-15
EP2001999A1 (en) 2008-12-17
US20130224332A1 (en) 2013-08-29
ES2387203T3 (es) 2012-09-17
US8460656B2 (en) 2013-06-11
JP2009532043A (ja) 2009-09-10
AU2007234177B2 (en) 2012-11-08
BRPI0709732A2 (pt) 2011-07-26
CA2647476A1 (en) 2007-10-11
JP5221516B2 (ja) 2013-06-26
US10041052B2 (en) 2018-08-07
MX2008012632A (es) 2008-10-13
BRPI0709732B1 (pt) 2017-06-06
US20110258714A1 (en) 2011-10-20
EP2001999B1 (en) 2012-05-16
PL2365064T3 (pl) 2015-05-29
AU2007234177A1 (en) 2007-10-11
EP2365064A1 (en) 2011-09-14
US20150024084A1 (en) 2015-01-22
ES2531434T3 (es) 2015-03-16
US9451783B2 (en) 2016-09-27
WO2007112739A1 (en) 2007-10-11
DK2365064T3 (en) 2015-03-30

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